Sgr023233 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr023233
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	RING-type domain-containing protein
Location	tig00000892: 1299299 .. 1302513 (+)
RNA-Seq Expression	Sgr023233
Synteny	Sgr023233

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTCTTCGGAAATCTCTATCGAAGGCGCCGAATTGTCTGATGCCATTGAGGACTTAAAAGTTTGCGATCAGGTTTTATTTTCTCTCCTTATTTCGAAGTTGATCTACTCAGATTTGCGCCCTTTCTTTATCCAGGAAAATTGGGGGTTTGAAACTTGACGTGAGAAATGGTTTCTCCGATTCGTGCTGAGGGTTCTAATAGGAAGTATTGTGTTCTGGAGTCTGAGATTGTTGATTTAGCCTTGTTTCCTTTCATTTTCAAGATTGCCGCACATCAGGAGGCTAATTGAAGTCTGAGATCGTTGATTTAGTCTTGTTTTTTTGTTTTAATTTTCAAGATTCCCGCACATCGGGAGGCTAATTTCTGTTATCAATCTCGTTTGAAGTACATGATTTAAACGAATTGACTTGATGCGCGTGTTTGCTTGTTTGGCTCTGAGGAATCCTAGGAAGCAGAAGCTATCGTTCATCTTCATTGAATTCTTTCTTCTGTATTTTTCTAATCCGTGAGTGTCCGGGCCGATTACCATTAATTGGATGATAGTTGATTCTGACTTGTGAACTTAGTCGCGAGCGCGCATGCCTCTTTACTTTCCAAGAAAAATTTTCTTCCAATTAGAGTTGCATATTTATGATTCTGTGAAAGTGTATCTTTTCGAGTGTTTGTTCTTTGCATACGCAGTTCGAGGATTGCTAACTTGCTGGAGTTTTCGTTTTTTAACTTTGTGTATTCAGGCGAAAATGGAAAAGAAGGTAGAGATTCAAGGAACGGGGCACGGTAGTCATGCTGGGGTTTGTGCAATTTGCTTAAATAAGATAGCTCTTCAAGAAACTGCCTTAGTGAGAGGTTGCGAGCACGCCTATTGGTCTGTTCTAATTTGATTAATCTTTCATCTATCGATTAATATTTTTTATAGCTTGACTTTCATCTGCTTCAATACATTAGGTAATTGTTGATCTGTTCTTTCCTGTATTTGTATGGGATTTTTTTTCGCAGTCAAATGTTGAAGTTCTATAGATGGTGCAATTCTAATGCTGCTTTTATCATATGTGTTTCATTTCGTTGTCGTTCTATGCTTGTATTGGAGATGGAGTTTTAAGTTCTTGCCCCATTGTATTCCTGGGAGTTTGCTAAACTTTATAGGAGAAAATTCACTATACCCATATTGCCTTTGGTGCAATGTTTCTAATTGTACTTCGGGTTGCTGGGATTTAATGGAAGAGATATGATGTTTTAGATTTATAACCTAAGAAAATCTTGGAGTTAGGGAAATATATGGTACAGAATCTGATGGTATCTATAATTTGATTTTCTTTGAAAAATAAAGTACCAGAAGCTAGCAAAACTCACTGATTTTTAGTTTGTTTAGTGTATTAATTATTGAAGTTCACATGCTTGCTTGGTGAAACTTGGAGAGGAACCTGTTATCCTCAGTATTCAGTGACTTATTGTGAACCGGACATGTTAGGTTGGTCAACGTGTCTGTGGTCGGACCAACACTTGTTAAGTATAATAGTTATGTGTTGGACACTTATTAAATTGGACACATATCAAATACTGTGTTGTTTAGTACAATAGACACATATGAAACACATGTTCTACACTTATTGGGCATTGGAACTCAAGTGTTCAAACATATTTTGAACGATAGACACGTGTCGTAGATTTTGAGAAAATGACGTGTCACAATGTTCATGTCCGTGCCTATGCTTCTTTGTTGTAAGCTAGTTACCCTTTCAGCATCTCCAAATCATGTCAGTACACACTTCTTTTATGCATTTCATGGCTGTTGAAAAGTTTGATTTTTATGTTTTTATACATGCATTGGCACTTTCTTAAGGGAACTTTTCCCCTACTGCTGTTGCAGGGAACACTTTATGTACTTTTCACTTTCAGCTGGGATACTTATCAGTAGTAATTAATCTAAAGCCAAAATCGAAGTGGCAAATGGTTAATGGAATTGCCAATTGTTTTCCAACCTCTGAAGTAATAGAAATTTTTTTCCTTGTTCTGTCTGAGGATTGAGAGTGGCTTTTTATAGTTGCACACATGGGAATTATCTTCGTTTATTTATTTATTCTTTTATTTTCGTGAAGTGCTACTTGCATCCTTCGATGGGCGTCATACACTCAGAATCCAACTTGTCCACAATGTAAACATCCATTTGAGTTCCTGATTGTGCATCGTTCTCTTGATGGGAGGTATGTCCATACATCTTTTTCAGTGATTTTCTTGCCATTAGCTGATTTGTTCTCTCAACATTTTTAGATACCCCTTCGTTTATTTAATTTTTATTTTTTTAATGTGATTAATACTACAAATGTGAAATCATTCCTCAAGTATATATCATTTGAGATATGTATGATTCACTTGCTGTATGCATTGGGAAAGGTAATAAAATCAATGTGCAATTTGTATGGCGGAAAACTATGGGAAAGGGCAGTGCCACTGAGAGTTTGACTCATTCATGCTTCTCTGTTCTTTTGAATGAACTTGAGGAAAAAACAAGATGAACTTTTCATATGATAATTATAAATAAAAAAATTATTATTTTGATTATGATAATTGGTACTTATATTCCAGAAAATGTGGAGTATTTAATGATAAAGTGAGAAACCAATACCCATTAATGAAGGCAGGCTTTAGTGTGTAATATGGATAATGAGATTATATTGTGCCAGTTATTTGTTCCTCATATGTTTTACCAGTTAGATTTTGTACTCTGCGTGGCCTATTATCTCTTTAAATTATTGTCCTTTTTGATTCGTATATTGCTTGGCACACTGTTAGCATCCATGACTACATGTTCGAGGAGAGTGTATGCCTGCTTTTGAGAGCTTCGTGGTATAAACCACTGATCGTTGAAGATCGGGAAGAAACTTATGATGATCCTGAGGAGTACTATCCTTATGAGGATGATGACGAAGAGCTTGAGGAAGCTTACCTGGGTGCATCATCAAGTCTCCGTATCGGCAATCGAAGGTGGGGAGACAATGGATATGTGAGGGCAGGTCGACAAGAAGCACGGCCAATTTTTAGGCCAACTGTTCCAGAGTTCGCTGCAGCTAGCTCATCACGCGAGCCTAAAAAGAAAGATGCTTCCACAGATAAAACAGGTCGACGAGCTAAAAGGGCCTTGAAACGGGAAGCTGCTGATAAAGCAGCAGCTGCTAAACATCAAGAACGATTAGCTAGGCTCGGCCGGAAGTGA

mRNA sequence

ATGACTTCTTCGGAAATCTCTATCGAAGGCGCCGAATTGTCTGATGCCATTGAGGACTTAAAAGTTTGCGATCAGGCGAAAATGGAAAAGAAGGTAGAGATTCAAGGAACGGGGCACGGTAGTCATGCTGGGGTTTGTGCAATTTGCTTAAATAAGATAGCTCTTCAAGAAACTGCCTTAGTGAGAGGTTGCGAGCACGCCTATTGTGCTACTTGCATCCTTCGATGGGCGTCATACACTCAGAATCCAACTTGTCCACAATGTAAACATCCATTTGAGTTCCTGATTGTGCATCGTTCTCTTGATGGGAGCATCCATGACTACATGTTCGAGGAGAGTGTATGCCTGCTTTTGAGAGCTTCGTGGTATAAACCACTGATCGTTGAAGATCGGGAAGAAACTTATGATGATCCTGAGGAGTACTATCCTTATGAGGATGATGACGAAGAGCTTGAGGAAGCTTACCTGGGTGCATCATCAAGTCTCCGTATCGGCAATCGAAGGTGGGGAGACAATGGATATGTGAGGGCAGGTCGACAAGAAGCACGGCCAATTTTTAGGCCAACTGTTCCAGAGTTCGCTGCAGCTAGCTCATCACGCGAGCCTAAAAAGAAAGATGCTTCCACAGATAAAACAGGTCGACGAGCTAAAAGGGCCTTGAAACGGGAAGCTGCTGATAAAGCAGCAGCTGCTAAACATCAAGAACGATTAGCTAGGCTCGGCCGGAAGTGA

Coding sequence (CDS)

ATGACTTCTTCGGAAATCTCTATCGAAGGCGCCGAATTGTCTGATGCCATTGAGGACTTAAAAGTTTGCGATCAGGCGAAAATGGAAAAGAAGGTAGAGATTCAAGGAACGGGGCACGGTAGTCATGCTGGGGTTTGTGCAATTTGCTTAAATAAGATAGCTCTTCAAGAAACTGCCTTAGTGAGAGGTTGCGAGCACGCCTATTGTGCTACTTGCATCCTTCGATGGGCGTCATACACTCAGAATCCAACTTGTCCACAATGTAAACATCCATTTGAGTTCCTGATTGTGCATCGTTCTCTTGATGGGAGCATCCATGACTACATGTTCGAGGAGAGTGTATGCCTGCTTTTGAGAGCTTCGTGGTATAAACCACTGATCGTTGAAGATCGGGAAGAAACTTATGATGATCCTGAGGAGTACTATCCTTATGAGGATGATGACGAAGAGCTTGAGGAAGCTTACCTGGGTGCATCATCAAGTCTCCGTATCGGCAATCGAAGGTGGGGAGACAATGGATATGTGAGGGCAGGTCGACAAGAAGCACGGCCAATTTTTAGGCCAACTGTTCCAGAGTTCGCTGCAGCTAGCTCATCACGCGAGCCTAAAAAGAAAGATGCTTCCACAGATAAAACAGGTCGACGAGCTAAAAGGGCCTTGAAACGGGAAGCTGCTGATAAAGCAGCAGCTGCTAAACATCAAGAACGATTAGCTAGGCTCGGCCGGAAGTGA

Protein sequence

MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETALVRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRASWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARLGRK

Homology

[Close]

BLAST of Sgr023233 vs. NCBI nr
Match: XP_022153003.1 (uncharacterized protein LOC111020607 [Momordica charantia])

HSP 1 Score: 444.1 bits (1141), Expect = 7.6e-121
Identity = 220/242 (90.91%), Postives = 229/242 (94.63%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           MTSS ISIEGAE+SDA+++L++CDQ K+ KKVEIQGTGHGSH G CAICLNKIALQETAL
Sbjct: 1   MTSSGISIEGAEMSDAVQELEICDQVKIGKKVEIQGTGHGSHDGACAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYC TCILRWASYTQ PTCPQCKHPFEFLIVHRSLDG IHDYMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCVTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY PL+VEDREETYDDPEEYYPYEDDDEELEE YLGASSSLRIGNRRWGDNGYVRAGRQ
Sbjct: 121 SWYIPLVVEDREETYDDPEEYYPYEDDDEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARPIFRP+VPEFAAASSSREP KKDAS DKTGRRAKRALKREAADKAAAAKHQE LARL
Sbjct: 181 EARPIFRPSVPEFAAASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARL 240

Query: 241 GR 243
           GR
Sbjct: 241 GR 242

[Close]

BLAST of Sgr023233 vs. NCBI nr
Match: XP_022958365.1 (uncharacterized protein LOC111459609 [Cucurbita moschata])

HSP 1 Score: 424.1 bits (1089), Expect = 8.2e-115
Identity = 212/243 (87.24%), Postives = 223/243 (91.77%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           M SS IS E AELSDA++ L+VC+Q +M KKVEIQGTGHGSH GVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASY +NPTCPQCKHPFEFLIVHRSLDGS+HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY PLIVEDREETY+DP+EYYPYEDDDEE EEAYLGASSSLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARP+ R TVP  A ASSSREP KKDAS DKTGRRAKRALKREAADKAAAAKHQERLAR 
Sbjct: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQERLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

[Close]

BLAST of Sgr023233 vs. NCBI nr
Match: KAG6605938.1 (hypothetical protein SDJN03_03255, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035883.1 hypothetical protein SDJN02_02682 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 422.2 bits (1084), Expect = 3.1e-114
Identity = 211/243 (86.83%), Postives = 222/243 (91.36%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           M SS IS E AELSDA++ L+VC+Q +M KKVEIQGTGHGSH GVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASY +NPTCPQCKHPFEFLIVHRSLDGS+HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY PLIVEDREETY+DP+EYYPYEDDDEE EEAYLGASSSLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARP+ R TVP  A ASSSREP KKDAS DKTGRRAKRALKREAADKAAAAKHQE LAR 
Sbjct: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

[Close]

BLAST of Sgr023233 vs. NCBI nr
Match: XP_022995136.1 (uncharacterized protein LOC111490767 [Cucurbita maxima])

HSP 1 Score: 419.9 bits (1078), Expect = 1.5e-113
Identity = 210/243 (86.42%), Postives = 221/243 (90.95%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           M SS IS E AELSDA++ L+VC+Q +M KKVEI GTGHGSH GVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIHGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASY +NPTCPQCKHPFEFLIVHRSLDGS+HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY PLIVEDREETY+DP+EYYPYEDDDEE EEAYLGASSSLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYVPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARP+ R TVP  A ASSSREP KKDAS DKTGRRAKRALKREAADKAAAAKHQE LAR 
Sbjct: 181 EARPVIRSTVPVHAGASSSREPIKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

[Close]

BLAST of Sgr023233 vs. NCBI nr
Match: XP_023533579.1 (uncharacterized protein LOC111795401 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419.1 bits (1076), Expect = 2.6e-113
Identity = 210/243 (86.42%), Postives = 221/243 (90.95%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           M SS IS E AELSDA++ L+VC+Q +M KKVEIQGTGHGSH GVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASY +NPTCPQCKHPFEFLIVHRSLDGS+HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY PLIVEDREETY+DP+EYYPYEDDDEE EEAYLGASSSLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARP+ R TVP  A ASSSREP KKD S DKTGRRAKRALKREAADKAAAAKHQE LAR 
Sbjct: 181 EARPVIRSTVPVHAGASSSREPIKKDDSKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

[Close]

BLAST of Sgr023233 vs. ExPASy Swiss-Prot
Match: Q99590 (Protein SCAF11 OS=Homo sapiens OX=9606 GN=SCAF11 PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 23/65 (35.38%), Postives = 34/65 (52.31%), Query Frame = 0


Query: 41  SHAGVCAICLNKIALQETALVRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRS 100
           S A  C ICLN +  +E      C H +C TCIL+WA      +CP  + PF+ +    +
Sbjct: 36  SEADRCPICLNCLLEKEVGFPESCNHVFCMTCILKWAETL--ASCPIDRKPFQAVFKFSA 95

Query: 101 LDGSI 106
           L+G +
Sbjct: 96  LEGYV 98

[Close]

BLAST of Sgr023233 vs. ExPASy Swiss-Prot
Match: P28990 (E3 ubiquitin-protein ligase ICP0 OS=Equine herpesvirus 1 (strain Ab4p) OX=31520 GN=63 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.6e-04
Identity = 24/51 (47.06%), Postives = 28/51 (54.90%), Query Frame = 0


Query: 46 CAICLNKIALQETALVRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLI 97
          C ICL   +    AL   C HA+C  CI RW    QNPTCP CK P E ++
Sbjct: 8  CPICLEDPSNYSMAL--PCLHAFCYVCITRW--IRQNPTCPLCKVPVESVV 54

[Close]

BLAST of Sgr023233 vs. ExPASy Swiss-Prot
Match: P84445 (E3 ubiquitin-protein ligase ICP0 OS=Equine herpesvirus 1 (strain V592) OX=310273 GN=ICP0 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.6e-04
Identity = 24/51 (47.06%), Postives = 28/51 (54.90%), Query Frame = 0


Query: 46 CAICLNKIALQETALVRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLI 97
          C ICL   +    AL   C HA+C  CI RW    QNPTCP CK P E ++
Sbjct: 8  CPICLEDPSNYSMAL--PCLHAFCYVCITRW--IRQNPTCPLCKVPVESVV 54

[Close]

BLAST of Sgr023233 vs. ExPASy Swiss-Prot
Match: B0F0H3 (Probable E3 ubiquitin-protein ligase makorin-2 OS=Xenopus laevis OX=8355 GN=mkrn2 PE=2 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 6.1e-04
Identity = 26/80 (32.50%), Postives = 40/80 (50.00%), Query Frame = 0


Query: 28  MEKKVEIQGTGHGSHAGVCAICLNKI------ALQETALVRGCEHAYCATCILRW--ASY 87
           ME+   +Q     S   VC+IC+ ++      A +   ++  C H YC TCI +W  A  
Sbjct: 216 MERAFAVQ----ASEGRVCSICMERVYEKQSPAQRRFRILSDCNHTYCLTCIRQWRCARQ 275

Query: 88  TQNP---TCPQCKHPFEFLI 97
             NP   +CP+C+   EF+I
Sbjct: 276 FDNPVIKSCPECRVISEFVI 291

[Close]

BLAST of Sgr023233 vs. ExPASy TrEMBL
Match: A0A6J1DGD3 (uncharacterized protein LOC111020607 OS=Momordica charantia OX=3673 GN=LOC111020607 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 3.7e-121
Identity = 220/242 (90.91%), Postives = 229/242 (94.63%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           MTSS ISIEGAE+SDA+++L++CDQ K+ KKVEIQGTGHGSH G CAICLNKIALQETAL
Sbjct: 1   MTSSGISIEGAEMSDAVQELEICDQVKIGKKVEIQGTGHGSHDGACAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYC TCILRWASYTQ PTCPQCKHPFEFLIVHRSLDG IHDYMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCVTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY PL+VEDREETYDDPEEYYPYEDDDEELEE YLGASSSLRIGNRRWGDNGYVRAGRQ
Sbjct: 121 SWYIPLVVEDREETYDDPEEYYPYEDDDEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARPIFRP+VPEFAAASSSREP KKDAS DKTGRRAKRALKREAADKAAAAKHQE LARL
Sbjct: 181 EARPIFRPSVPEFAAASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARL 240

Query: 241 GR 243
           GR
Sbjct: 241 GR 242

[Close]

BLAST of Sgr023233 vs. ExPASy TrEMBL
Match: A0A6J1H396 (uncharacterized protein LOC111459609 OS=Cucurbita moschata OX=3662 GN=LOC111459609 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 4.0e-115
Identity = 212/243 (87.24%), Postives = 223/243 (91.77%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           M SS IS E AELSDA++ L+VC+Q +M KKVEIQGTGHGSH GVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASY +NPTCPQCKHPFEFLIVHRSLDGS+HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY PLIVEDREETY+DP+EYYPYEDDDEE EEAYLGASSSLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARP+ R TVP  A ASSSREP KKDAS DKTGRRAKRALKREAADKAAAAKHQERLAR 
Sbjct: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQERLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

[Close]

BLAST of Sgr023233 vs. ExPASy TrEMBL
Match: A0A6J1K158 (uncharacterized protein LOC111490767 OS=Cucurbita maxima OX=3661 GN=LOC111490767 PE=4 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 7.5e-114
Identity = 210/243 (86.42%), Postives = 221/243 (90.95%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           M SS IS E AELSDA++ L+VC+Q +M KKVEI GTGHGSH GVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIHGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASY +NPTCPQCKHPFEFLIVHRSLDGS+HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY PLIVEDREETY+DP+EYYPYEDDDEE EEAYLGASSSLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYVPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARP+ R TVP  A ASSSREP KKDAS DKTGRRAKRALKREAADKAAAAKHQE LAR 
Sbjct: 181 EARPVIRSTVPVHAGASSSREPIKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

[Close]

BLAST of Sgr023233 vs. ExPASy TrEMBL
Match: A0A6J1FXD4 (uncharacterized protein LOC111448431 OS=Cucurbita moschata OX=3662 GN=LOC111448431 PE=4 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 2.8e-113
Identity = 209/241 (86.72%), Postives = 220/241 (91.29%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           MTSS IS++ AELSDA++DLKVCDQ +M KK+EIQGTGHGSH G CAICLNKI+L ETAL
Sbjct: 1   MTSSGISLKSAELSDAVQDLKVCDQVEMWKKLEIQGTGHGSHDGACAICLNKISLPETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYC TCILRWASYTQ PTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCTTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY+PLIV+DREETYDDPEEYYPYED DEELEEAYLGAS SLRIGNRRWGDNGYVRAGRQ
Sbjct: 121 SWYEPLIVKDREETYDDPEEYYPYEDQDEELEEAYLGASPSLRIGNRRWGDNGYVRAGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARP+ R   PEF+ ASSSREP  KDAS DKTGRRAKRALKREAADKAAAAKHQE LAR 
Sbjct: 181 EARPVLR---PEFSGASSSREPTTKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 238

Query: 241 G 242
           G
Sbjct: 241 G 238

[Close]

BLAST of Sgr023233 vs. ExPASy TrEMBL
Match: A0A6J1JFP2 (uncharacterized protein LOC111484597 OS=Cucurbita maxima OX=3661 GN=LOC111484597 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 8.6e-110
Identity = 206/241 (85.48%), Postives = 218/241 (90.46%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           MTSS IS++ AELSDA++DLKVCDQ +M KK+E+Q TGHGSH G CAICLNKI+L ETAL
Sbjct: 1   MTSSGISLKSAELSDAVQDLKVCDQEEMWKKLEMQRTGHGSH-GSCAICLNKISLPETAL 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           VRGCEHAYC TCILRWASYTQ PTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCTTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQ 180
           SWY+PLIV+DREETYDDPEEYYPYED DEELEEAYLG S SLRIGNRRWGDNGYVRAGRQ
Sbjct: 121 SWYEPLIVKDREETYDDPEEYYPYEDQDEELEEAYLGDSPSLRIGNRRWGDNGYVRAGRQ 180

Query: 181 EARPIFRPTVPEFAAASSSREPKKKDASTDKTGRRAKRALKREAADKAAAAKHQERLARL 240
           EARP+ R   PEF+ ASSSREP  KDAS DKTGRRAKRALKREAADKAAAAKHQE LAR 
Sbjct: 181 EARPVLR---PEFSGASSSREPTTKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 237

Query: 241 G 242
           G
Sbjct: 241 G 237

[Close]

BLAST of Sgr023233 vs. TAIR 10
Match: AT5G19430.1 (RING/U-box superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 7.2e-69
Identity = 144/256 (56.25%), Postives = 184/256 (71.88%), Query Frame = 0


Query: 1   MTSSEISIEGAELSDAIEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETAL 60
           M+S    +   ++ D I+DL + +Q K + + EI     G+H G CAICL++I LQETA+
Sbjct: 1   MSSVTEIVSEQQVIDGIQDLALQNQDKKKIQEEIHEPSFGNHGGCCAICLSEIPLQETAM 60

Query: 61  VRGCEHAYCATCILRWASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120
           V+GCEH YC TCILRWAS  ++PTCPQCKHPF+FL VHR+LDGSI D++FEESVCLLLRA
Sbjct: 61  VKGCEHTYCVTCILRWASCKESPTCPQCKHPFDFLSVHRALDGSIEDFLFEESVCLLLRA 120

Query: 121 SWYKPLIVEDREE----TYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVR 180
           SW+ PL V ++       +DD +  Y YED+D++L+E YL   SSLRIGNRRWG NG+VR
Sbjct: 121 SWFVPLDVVEQASYSHGYHDDFDIPYDYEDEDDDLDEFYL-HGSSLRIGNRRWGGNGFVR 180

Query: 181 AGRQEARPIFR----PTVPEFAAASSSREPKKKDA-STDKTGRRAKRALKREAADKA--- 240
           +GRQEARP+ R     +    ++ SSS EPK K   +T+ TGRRAKRA+KREAA+KA   
Sbjct: 181 SGRQEARPVQRYSGSGSSSSSSSGSSSSEPKDKQVKTTNTTGRRAKRAMKREAANKAAEV 240

Query: 241 -AAAKHQERLARLGRK 244
            AAAKH+  L RLGRK
Sbjct: 241 TAAAKHEAHLVRLGRK 255

[Close]

BLAST of Sgr023233 vs. TAIR 10
Match: AT5G19430.2 (RING/U-box superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 2.6e-66
Identity = 138/233 (59.23%), Postives = 173/233 (74.25%), Query Frame = 0


Query: 24  DQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETALVRGCEHAYCATCILRWASYTQNP 83
           D+ K+++  EI     G+H G CAICL++I LQETA+V+GCEH YC TCILRWAS  ++P
Sbjct: 11  DKKKIQE--EIHEPSFGNHGGCCAICLSEIPLQETAMVKGCEHTYCVTCILRWASCKESP 70

Query: 84  TCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRASWYKPLIVEDREE----TYDDPE 143
           TCPQCKHPF+FL VHR+LDGSI D++FEESVCLLLRASW+ PL V ++       +DD +
Sbjct: 71  TCPQCKHPFDFLSVHRALDGSIEDFLFEESVCLLLRASWFVPLDVVEQASYSHGYHDDFD 130

Query: 144 EYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFR----PTVPEFAA 203
             Y YED+D++L+E YL   SSLRIGNRRWG NG+VR+GRQEARP+ R     +    ++
Sbjct: 131 IPYDYEDEDDDLDEFYL-HGSSLRIGNRRWGGNGFVRSGRQEARPVQRYSGSGSSSSSSS 190

Query: 204 ASSSREPKKKDA-STDKTGRRAKRALKREAADKA----AAAKHQERLARLGRK 244
            SSS EPK K   +T+ TGRRAKRA+KREAA+KA    AAAKH+  L RLGRK
Sbjct: 191 GSSSSEPKDKQVKTTNTTGRRAKRAMKREAANKAAEVTAAAKHEAHLVRLGRK 240

[Close]

BLAST of Sgr023233 vs. TAIR 10
Match: AT5G12310.1 (RING/U-box superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 4.9e-65
Identity = 138/243 (56.79%), Postives = 171/243 (70.37%), Query Frame = 0


Query: 17  IEDLKVCDQAKMEKKVEIQGTGHGSHAGVCAICLNKIALQETALVRGCEHAYCATCILRW 76
           ++ L + DQ + +          G+H G CAICL+ I LQETA+V+GCEHAYC TCILRW
Sbjct: 14  VDVLPLQDQDEKKTPQVFNELSFGNHGGCCAICLDTIPLQETAMVKGCEHAYCVTCILRW 73

Query: 77  ASYTQNPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRASWYKPLIVEDR----- 136
           ASY + PTCPQCK PF+FL VHR+LDGS+ D+MFEESVCLLLRASW++PL   +R     
Sbjct: 74  ASYKEKPTCPQCKLPFDFLNVHRALDGSVEDFMFEESVCLLLRASWFQPLEAVERVSDND 133

Query: 137 --EETYDDPEEYYPYEDDDEELEEAYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPT 196
                +D P EY   ++DD++L+E Y+   SSLR+GNRRWGDNG+VRAGRQEARP+    
Sbjct: 134 NFNYDFDIPPEYEEEDEDDDDLDEFYM-QGSSLRLGNRRWGDNGFVRAGRQEARPVQHKN 193

Query: 197 VPEFA-----AASSSREPKKKDASTDKTGRRAKRALKREAADKA----AAAKHQERLARL 244
               A     A+SSS EPK+K +S   TGRRAKRA+KREAA+KA    AAAKH+  L RL
Sbjct: 194 RGGQASGSEPASSSSCEPKEKTSSA-ITGRRAKRAMKREAANKAAEAVAAAKHEALLVRL 253

[Close]

BLAST of Sgr023233 vs. TAIR 10
Match: AT3G30460.1 (RING/U-box superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 8.2e-04
Identity = 20/49 (40.82%), Postives = 23/49 (46.94%), Query Frame = 0


Query: 45  VCAICLNKIALQETALVRGCEHAYCATCILRWASYTQNPTCPQCKHPFE 94
           VCAIC  ++A  E      C H Y   CI  W S     TCP C+H  E
Sbjct: 96  VCAICREELAANERLSELPCRHYYHKECISNWLS--NRNTCPLCRHNVE 142

The following BLAST results are available for this feature:

BLAST of Sgr023233 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153003.1	7.6e-121	90.91	uncharacterized protein LOC111020607 [Momordica charantia]	[more]
XP_022958365.1	8.2e-115	87.24	uncharacterized protein LOC111459609 [Cucurbita moschata]	[more]
KAG6605938.1	3.1e-114	86.83	hypothetical protein SDJN03_03255, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022995136.1	1.5e-113	86.42	uncharacterized protein LOC111490767 [Cucurbita maxima]	[more]
XP_023533579.1	2.6e-113	86.42	uncharacterized protein LOC111795401 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sgr023233 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99590	1.1e-05	35.38	Protein SCAF11 OS=Homo sapiens OX=9606 GN=SCAF11 PE=1 SV=2	[more]
P28990	1.6e-04	47.06	E3 ubiquitin-protein ligase ICP0 OS=Equine herpesvirus 1 (strain Ab4p) OX=31520 ...	[more]
P84445	1.6e-04	47.06	E3 ubiquitin-protein ligase ICP0 OS=Equine herpesvirus 1 (strain V592) OX=310273...	[more]
B0F0H3	6.1e-04	32.50	Probable E3 ubiquitin-protein ligase makorin-2 OS=Xenopus laevis OX=8355 GN=mkrn...	[more]

BLAST of Sgr023233 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGD3	3.7e-121	90.91	uncharacterized protein LOC111020607 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1H396	4.0e-115	87.24	uncharacterized protein LOC111459609 OS=Cucurbita moschata OX=3662 GN=LOC1114596...	[more]
A0A6J1K158	7.5e-114	86.42	uncharacterized protein LOC111490767 OS=Cucurbita maxima OX=3661 GN=LOC111490767...	[more]
A0A6J1FXD4	2.8e-113	86.72	uncharacterized protein LOC111448431 OS=Cucurbita moschata OX=3662 GN=LOC1114484...	[more]
A0A6J1JFP2	8.6e-110	85.48	uncharacterized protein LOC111484597 OS=Cucurbita maxima OX=3661 GN=LOC111484597...	[more]

BLAST of Sgr023233 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19430.1	7.2e-69	56.25	RING/U-box superfamily protein	[more]
AT5G19430.2	2.6e-66	59.23	RING/U-box superfamily protein	[more]
AT5G12310.1	4.9e-65	56.79	RING/U-box superfamily protein	[more]
AT3G30460.1	8.2e-04	40.82	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 46..88 e-value: 9.1E-6 score: 35.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 46..89 e-value: 1.5E-8 score: 34.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 46..89 score: 12.518784
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 33..95 e-value: 2.2E-11 score: 44.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 198..243
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 189..243
None	No IPR available	PANTHER	PTHR47361:SF4	RING/U-BOX SUPERFAMILY PROTEIN	coord: 6..243
None	No IPR available	PANTHER	PTHR47361	RING/U-BOX SUPERFAMILY PROTEIN	coord: 6..243
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 45..96
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 64..73

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr023233.1	Sgr023233.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding