Sgr023230 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr023230
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	subtilisin-like protease SBT4.3
Location	tig00000892: 1241702 .. 1243742 (-)
RNA-Seq Expression	Sgr023230
Synteny	Sgr023230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAAGAATCGGCCCATTTTGTTTCAGTTCATGGCTGCTCTTTTAGCTGCCGTTTGCTCGTCGACGTTGTGCAGTGCTGCCATTAATGGCGATAGAAAGGTTCATATTGTGTATATGGGGTCGCTCAGAAACATGGCGATGGCACAATCATCTCACCATCTGAATCTGCTTCAATCGGTTGTTGGAACCTCTTCTGAAACGGAAGGTTTATACGTTAGGAGCTATGGAAGGAGCTTCAATGGCTTCGCTGCTGAACTCACCGACAGTGAGAGAGAGAAGCTTGCCGCCATGGAAGGAGTGGTCTCAGTTTTCCCAAGCAAGATACTAAAAACACAAACGACAAGGTCATGGGATTATGTGGGCTTCCGTCGTAAAATCAAGAGAGATGTCGCCGGTGAAACTGATGTCATAATAGGGTCCATTGACACTGGGATTTGGTCGGAATCAGAGAGCTTCAACGATGATGGTTAGGCCCGCCACCGCAACGGTGGAAAGGCGCCTGTGTCGGCGGAGAGAACTTCACCTGCAACAACAAGATCATTGGAGCACGTTATTACTCGTTTTCCTCAGCGAGGGACAGCATCGGCCATGGATCTCACACTGCCTCCACAGCCGCCAGAAGAGCAGTAAAACATGCCAGTTTTTATGGAATAGCGGCCGGAGTTGCGAGAGGAGCCGTCCCTTCTGCAAGAATCGCCGCTTACAAAGTCTGTGATTCCGGTTGCCACGAAACAGATGTCTTGGCAGCCTTCGACGACGCCATCGCCGACGGAGTTGATCTCATTACAATATCAATCAGCGGCGTCTGTGCAACTCCATTTAAGTACGATGGCATTGCAATCGGCTCCTTTCACGCCATGGCCAGAGGAATACTGACAGTCCACTCTGCCGGCAGCGACGGTCCGGCGGAAGGCTCAGTGGGAAGCGTAGCTCCATGGCTGTTTCTGTCGACGCCATTAGGCATTGTCCTCCTGGGACTTCAGCACAGTGAACTCCTTCCCTCAAAACAGGATGAAACTTCCATTGATTTACGGAACAAATGCTTCAAGAACTTGTTCCCGACATCTTTCTGAAACATGTTCTGAAGCGTGTCTGGATCATGAGTTGGTGAAGGGGAAGATTGTTCAGTGCATGTCTTTTTGATGGAGCTTCTGAAAAGTGAAGCCATTAAAGACGCTTCTGCTCCAAAAGTTGCTCAGTTCTCCGGCCGTGGCCCCAACTCCTTGATGCCGGAGATCATGAAGCCGGACATATCCGCCCCCGGCGTCGAAATTCTCTCCGCCTTTTCACCTGTAGTTTCTCCTTCCGATGTTCTTGGAGACCGAAGATCTGTCAAGTACTCTATAATGTCTGGGACTTCCATGTCTTGCCCCATGTCGCCGGCGTGGCTGCTTATGTGAAGAACTTCCATCAGAACTGGTCTCCGTCGGCGATCAAATCAGCCATTATGACGACGGCCAAGCAAATCATCAGCACCGACGGCATGCACATCGGAGAGTGTCTTTATGGGTCTGGTCAGATCAATCCGAAACAAGCTATTAAACCTGGCCTTGTCTATGAAACTTCCGAAGAAGATTACGTAAACTTGCTCCGCGGTAATGGCTATGATTCAAAGGCAATCGAACTCATTTCAGGAGACAACAGTACTTGTTCAACAAATGCGACGACGTTTCCACCCAAAGATCTCAATTATCCTGCAATGGTGGCTCAAGTTTCGCCGACGCAGCCGTTTGTAGTTAAGTTTCAGAGAACAGTTACGAACGTCGGGGTTGCAAACTCCACCTACAGCCGAATTCCTGCCGTTTCTGAAGTTTATCTTCTCAAGTCACCGGAAAAGCTCAACACGAGCGTTGAGCCTCAAGAAGTGTCGTTTGAGGCGTTGAATGAGAAGAAGAGTTTCACGGTGACGGTGGCCGGAGGGATAATTCCGGCGGGAACAGTGTTTTCTACGTCGTTGGTTTGGAGTGATGGAATCCACAAGGTGAGAAGTCCGATTGTGGTGAAAGTCATATCACAATCCAGTCCTCCCTCGAACTGA

mRNA sequence

ATGGCTAAGAATCGGCCCATTTTGTTTCAGTTCATGGCTGCTCTTTTAGCTGCCGTTTGCTCGTCGACGTTGTGCAGTGCTGCCATTAATGGCGATAGAAAGGTTCATATTGTGTATATGGGGTCGCTCAGAAACATGGCGATGGCACAATCATCTCACCATCTGAATCTGCTTCAATCGGTTGTTGGAACCTCTTCTGAAACGGAAGGTTTATACGTTAGGAGCTATGGAAGGAGCTTCAATGGCTTCGCTGCTGAACTCACCGACAGTGAGAGAGAGAAGCTTGCCGCCATGGAAGGAGTGGTCTCAGTTTTCCCAAGCAAGATACTAAAAACACAAACGACAAGGTCATGGGATTATGTGGGCTTCCGTCGTAAAATCAAGAGAGATGTCGCCGGTGAAACTGATGTCATAATAGGGTCCATTGACACTGGGATTTGGTCGGAATCAGAGAGCTTCAACGATGATGCGAGGGACAGCATCGGCCATGGATCTCACACTGCCTCCACAGCCGCCAGAAGAGCAGTAAAACATGCCAGTTTTTATGGAATAGCGGCCGGAGTTGCGAGAGGAGCCGTCCCTTCTGCAAGAATCGCCGCTTACAAAGTCTGTGATTCCGGTTGCCACGAAACAGATGTCTTGGCAGCCTTCGACGACGCCATCGCCGACGGAGTTGATCTCATTACAATATCAATCAGCGGCGTCTGTGCAACTCCATTTAAGTACGATGGCATTGCAATCGGCTCCTTTCACGCCATGGCCAGAGGAATACTGACAGTCCACTCTGCCGGCAGCGACGGTCCGGCGGAAGGCTCAGTGGGAAGCGTAGCTCCATGGCTGTTTCTGTCGACGCCATTAGGCATTGTCCTCCTGGGACTTCAGCACAGTGAACTCCTTCCCTCAAAACAGGATGAAACTTCCATTGATTTACGGAACAAATGCTTCAAGAACTTACGCTTCTGCTCCAAAAGTTGCTCAGTTCTCCGGCCGTGGCCCCAACTCCTTGATGCCGGAGATCATGAAGCCGGACATATCCGCCCCCGGCGTCGAAATTCTCTCCGCCTTTTCACCTGTAGTTTCTCCTTCCGATGTTCTTGGAGACCGAAGATCTGTCAAGTACTCTATAATGTCTGGGACTTCCATGTCTTGCCCCATGTCGCCGGCGTGGCTGCTTATGTGAAGAACTTCCATCAGAACTGGTCTCCGTCGGCGATCAAATCAGCCATTATGACGACGGCCAAGCAAATCATCAGCACCGACGGCATGCACATCGGAGAGTGTCTTTATGGGTCTGGTCAGATCAATCCGAAACAAGCTATTAAACCTGGCCTTGTCTATGAAACTTCCGAAGAAGATTACGTAAACTTGCTCCGCGGTAATGGCTATGATTCAAAGGCAATCGAACTCATTTCAGGAGACAACAGTACTTGTTCAACAAATGCGACGACGTTTCCACCCAAAGATCTCAATTATCCTGCAATGGTGGCTCAAGTTTCGCCGACGCAGCCGTTTGTAGTTAAGTTTCAGAGAACAGTTACGAACGTCGGGGTTGCAAACTCCACCTACAGCCGAATTCCTGCCGTTTCTGAAGTTTATCTTCTCAAGTCACCGGAAAAGCTCAACACGAGCGTTGAGCCTCAAGAAGTGTCGTTTGAGGCGTTGAATGAGAAGAAGAGTTTCACGGTGACGGTGGCCGGAGGGATAATTCCGGCGGGAACAGTGTTTTCTACGTCGTTGGTTTGGAGTGATGGAATCCACAAGGTGAGAAGTCCGATTGTGGTGAAAGTCATATCACAATCCAGTCCTCCCTCGAACTGA

Coding sequence (CDS)

ATGGCTAAGAATCGGCCCATTTTGTTTCAGTTCATGGCTGCTCTTTTAGCTGCCGTTTGCTCGTCGACGTTGTGCAGTGCTGCCATTAATGGCGATAGAAAGGTTCATATTGTGTATATGGGGTCGCTCAGAAACATGGCGATGGCACAATCATCTCACCATCTGAATCTGCTTCAATCGGTTGTTGGAACCTCTTCTGAAACGGAAGGTTTATACGTTAGGAGCTATGGAAGGAGCTTCAATGGCTTCGCTGCTGAACTCACCGACAGTGAGAGAGAGAAGCTTGCCGCCATGGAAGGAGTGGTCTCAGTTTTCCCAAGCAAGATACTAAAAACACAAACGACAAGGTCATGGGATTATGTGGGCTTCCGTCGTAAAATCAAGAGAGATGTCGCCGGTGAAACTGATGTCATAATAGGGTCCATTGACACTGGGATTTGGTCGGAATCAGAGAGCTTCAACGATGATGCGAGGGACAGCATCGGCCATGGATCTCACACTGCCTCCACAGCCGCCAGAAGAGCAGTAAAACATGCCAGTTTTTATGGAATAGCGGCCGGAGTTGCGAGAGGAGCCGTCCCTTCTGCAAGAATCGCCGCTTACAAAGTCTGTGATTCCGGTTGCCACGAAACAGATGTCTTGGCAGCCTTCGACGACGCCATCGCCGACGGAGTTGATCTCATTACAATATCAATCAGCGGCGTCTGTGCAACTCCATTTAAGTACGATGGCATTGCAATCGGCTCCTTTCACGCCATGGCCAGAGGAATACTGACAGTCCACTCTGCCGGCAGCGACGGTCCGGCGGAAGGCTCAGTGGGAAGCGTAGCTCCATGGCTGTTTCTGTCGACGCCATTAGGCATTGTCCTCCTGGGACTTCAGCACAGTGAACTCCTTCCCTCAAAACAGGATGAAACTTCCATTGATTTACGGAACAAATGCTTCAAGAACTTACGCTTCTGCTCCAAAAGTTGCTCAGTTCTCCGGCCGTGGCCCCAACTCCTTGATGCCGGAGATCATGAAGCCGGACATATCCGCCCCCGGCGTCGAAATTCTCTCCGCCTTTTCACCTGTAGTTTCTCCTTCCGATGTTCTTGGAGACCGAAGATCTGTCAAGTACTCTATAATGTCTGGGACTTCCATGTCTTGCCCCATGTCGCCGGCGTGGCTGCTTATGTGAAGAACTTCCATCAGAACTGGTCTCCGTCGGCGATCAAATCAGCCATTATGACGACGGCCAAGCAAATCATCAGCACCGACGGCATGCACATCGGAGAGTGTCTTTATGGGTCTGGTCAGATCAATCCGAAACAAGCTATTAAACCTGGCCTTGTCTATGAAACTTCCGAAGAAGATTACGTAAACTTGCTCCGCGGTAATGGCTATGATTCAAAGGCAATCGAACTCATTTCAGGAGACAACAGTACTTGTTCAACAAATGCGACGACGTTTCCACCCAAAGATCTCAATTATCCTGCAATGGTGGCTCAAGTTTCGCCGACGCAGCCGTTTGTAGTTAAGTTTCAGAGAACAGTTACGAACGTCGGGGTTGCAAACTCCACCTACAGCCGAATTCCTGCCGTTTCTGAAGTTTATCTTCTCAAGTCACCGGAAAAGCTCAACACGAGCGTTGAGCCTCAAGAAGTGTCGTTTGAGGCGTTGAATGAGAAGAAGAGTTTCACGGTGACGGTGGCCGGAGGGATAATTCCGGCGGGAACAGTGTTTTCTACGTCGTTGGTTTGGAGTGATGGAATCCACAAGGTGAGAAGTCCGATTGTGGTGAAAGTCATATCACAATCCAGTCCTCCCTCGAACTGA

Protein sequence

MAKNRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSVVGTSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFRRKIKRDVAGETDVIIGSIDTGIWSESESFNDDARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDSGCHETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGPAEGSVGSVAPWLFLSTPLGIVLLGLQHSELLPSKQDETSIDLRNKCFKNLRFCSKSCSVLRPWPQLLDAGDHEAGHIRPRRRNSLRLFTCSFSFRCSWRPKICQVLYNVWDFHVLPHVAGVAAYVKNFHQNWSPSAIKSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYDSKAIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPTQPFVVKFQRTVTNVGVANSTYSRIPAVSEVYLLKSPEKLNTSVEPQEVSFEALNEKKSFTVTVAGGIIPAGTVFSTSLVWSDGIHKVRSPIVVKVISQSSPPSN

Homology

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BLAST of Sgr023230 vs. NCBI nr
Match: XP_022995031.1 (subtilisin-like protease SBT4.3 [Cucurbita maxima])

HSP 1 Score: 585.9 bits (1509), Expect = 4.1e-163
Identity = 359/744 (48.25%), Postives = 444/744 (59.68%), Query Frame = 0


Query: 4   NRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSVVG 63
           N PILF F+AALLAA+ SS     A   +RK HIVYMG+++N AMA+S+HHLNLL+SV+G
Sbjct: 5   NFPILFSFIAALLAAILSS-----ANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIG 64

Query: 64  TSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGF 123
           TSS TEG Y+RSYGRSFNGF A+LT +E E+LAAM+GVVSVF SK+ KTQTTRSWDY+GF
Sbjct: 65  TSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGF 124

Query: 124 RRKIKRDVAGETDVIIGSIDTGIWSESESFNDD--------------------------- 183
             K  R++AGETDVIIGSIDTGIW E ESFND+                           
Sbjct: 125 PAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIG 184

Query: 184 --------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDSGC 243
                   ARD++GHGSHTASTAA +  K   FYG+A G ARGAVPS+R+A YKVC+  C
Sbjct: 185 ARFYTSMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPDC 244

Query: 244 HETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGP 303
            E ++LAAFDDAIADGVDLITISI GV    F++D +AIGS+H+MA+GILTV SAG+ GP
Sbjct: 245 LEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGP 304

Query: 304 AEGSVGSVAPWLFLSTPL--------------GIVLLGLQHSELLPSKQDE---TSIDLR 363
            +G+VGSV PW+F                   G  + G   +   P++       +   R
Sbjct: 305 VDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASR 364

Query: 364 NKCFKNLRFCSKSC----------------------------------------SVLRPW 423
           N   KN   CS  C                                        S + P+
Sbjct: 365 NCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPF 424

Query: 424 PQL-LDAGDHE--AGHIRPRRRNSLRLFTCSFSFRCSWRPKICQVLY---NVWDFHVL-- 483
           P + L   D++  A +       ++ +F  S + +  + P I        N+W   +L  
Sbjct: 425 PAIALKMADYKSVANYAISATNPNVTIFR-SVATKDPYAPTIADFSSRGPNMWMLEILKP 484

Query: 484 -----------------------------------------PHVAGVAAYVKNFHQNWSP 543
                                                    PHVAGVAAYVK+FH NWSP
Sbjct: 485 DIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSP 544

Query: 544 SAIKSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGY 603
           +AIKSAIMTTAKQI+ TDG+ I E LYGSG I+P +AI+PGLVYE  E+D++N+L   GY
Sbjct: 545 AAIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGY 604

Query: 604 DSKAIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPTQPFVVKFQRTVTNVGVANSTY 606
           DSK +E  +G++S CS   T F  +DLNYPAMVA V P +PFVVKFQRTVTNVGVANSTY
Sbjct: 605 DSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTY 664

[Close]

BLAST of Sgr023230 vs. NCBI nr
Match: XP_022958362.1 (subtilisin-like protease SBT4.3 [Cucurbita moschata])

HSP 1 Score: 584.3 bits (1505), Expect = 1.2e-162
Identity = 365/743 (49.13%), Postives = 446/743 (60.03%), Query Frame = 0


Query: 2   AKNRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSV 61
           A N PILF F+AALLAA+ +S     A   +RK HIVYMG+++N AMA+S+HHLNLL+SV
Sbjct: 3   AINFPILFTFIAALLAAIFTS-----ANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSV 62

Query: 62  VGTSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYV 121
           +GTSS TEG Y+RSYGRSFNGF AELT  E E+LAAM+GVVSVF SK LKT+TTRSWD++
Sbjct: 63  IGTSSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFL 122

Query: 122 GFRRKIKRDVAGETDVIIGSIDTGIWSESESFNDD------------------------- 181
           GF++  KR+++GE DVIIGSID+GIW ESESFNDD                         
Sbjct: 123 GFQQP-KRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCNHKV 182

Query: 182 ----------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDS 241
                     ARD  GHG+HTASTA  ++V    FYGIA GVARGAVPS+R+A Y  C  
Sbjct: 183 IGARYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSP 242

Query: 242 GCHETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSD 301
            C + ++LAAFDDAIADGVD+ITIS+ G   +  + D IAIG++HAM +GILTV SAG+D
Sbjct: 243 SCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGND 302

Query: 302 GPAEGSVGSVAPWLF----LSTPLGIV----------LLGLQHSELLPSKQDET--SIDL 361
           GP  GSV SVAPWLF     +T   IV          + G   +   P+K        + 
Sbjct: 303 GPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNA 362

Query: 362 RNKC-FKNLRFCS-----------KSCSVLRPWPQLLDAGDHEA---------------- 421
              C F+N   CS             C       + +DAG   A                
Sbjct: 363 SKSCSFQNPEVCSCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPL 422

Query: 422 ----------------------GHIRPRR----RNSLRLFTCSFSFRCS-------WRPK 481
                                  H+R  +    ++        FS R          +P 
Sbjct: 423 PATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPEIMKPD 482

Query: 482 IC----QVLYNV---------------WDFHVL-------PHVAGVAAYVKNFHQNWSPS 541
           I     ++L +V                +F ++       PHVAG+AAYVK+FH NWSPS
Sbjct: 483 IAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPS 542

Query: 542 AIKSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYD 601
           AIKSAIMTTA++I +TDG   GE L+GSGQINPKQAI+PGLVYE  E+DY+  L GNG+D
Sbjct: 543 AIKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKFLCGNGFD 602

Query: 602 SKAIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPTQPFVVKFQRTVTNVGVANSTY- 606
           SK++  ISG+ S CS  +T F P+DLNYPAMV +V P +PFVVKFQRTVTNVG ANSTY 
Sbjct: 603 SKSVRGISGNKSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNVGTANSTYK 662

[Close]

BLAST of Sgr023230 vs. NCBI nr
Match: KAG6605933.1 (Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 583.6 bits (1503), Expect = 2.0e-162
Identity = 363/743 (48.86%), Postives = 446/743 (60.03%), Query Frame = 0


Query: 2   AKNRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSV 61
           A N PI F F+AALLAA+ +S     A   +RK HIVYMG+++N AMA+S+HHLNLL+SV
Sbjct: 3   ANNFPIFFSFIAALLAAIFTS-----ANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSV 62

Query: 62  VGTSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYV 121
           +GTSS TEG Y+RSYGRSFNGF AELT  E E+LAAM+GVVSVF SK LKT+TTRSWD++
Sbjct: 63  IGTSSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFL 122

Query: 122 GFRRKIKRDVAGETDVIIGSIDTGIWSESESFNDD------------------------- 181
           GF++  KR++AGE DVIIGSID+GIW ESESFND+                         
Sbjct: 123 GFQQP-KRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCNHKV 182

Query: 182 ----------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDS 241
                     ARD  GHG+HTASTA  ++V    FYGIA GVARGAVPS+R+A Y  C  
Sbjct: 183 IGARYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSP 242

Query: 242 GCHETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSD 301
            C + ++LAAFDDAIADGVD+ITIS+ G   +  + D IAIG++HAM +GILTV SAG+D
Sbjct: 243 SCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGND 302

Query: 302 GPAEGSVGSVAPWLF----LSTPLGIV----------LLGLQHSELLPSKQDET--SIDL 361
           GP  GSV SVAPWLF     +T   IV          + G   +   P+K        + 
Sbjct: 303 GPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNA 362

Query: 362 RNKC-FKNLRFCS-----------KSCSVLRPWPQLLDAGDHEA---------------- 421
              C F+N   CS             C       + +DAG   A                
Sbjct: 363 SKSCSFQNPEVCSCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPL 422

Query: 422 ----------------------GHIRPRR----RNSLRLFTCSFSFRCS-------WRPK 481
                                  H+R  +    ++        FS R          +P 
Sbjct: 423 PAIALNFTAYEQVTNYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPEIMKPD 482

Query: 482 IC----QVLYNV---------------WDFHVL-------PHVAGVAAYVKNFHQNWSPS 541
           I     ++L +V                +F ++       PHVAG+AAYVK+FH NWSPS
Sbjct: 483 IAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPS 542

Query: 542 AIKSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYD 601
           AIKSAIMTTA++I +TDG   GE L+GSGQINPKQAI+PGLVYE  E+DY+  L GNG+D
Sbjct: 543 AIKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKFLCGNGFD 602

Query: 602 SKAIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPTQPFVVKFQRTVTNVGVANSTY- 606
           SK++  ISG+ S CS  +T F P+DLNYPAMV +V P +PFVVKFQR VTNVG ANSTY 
Sbjct: 603 SKSVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRIVTNVGTANSTYK 662

[Close]

BLAST of Sgr023230 vs. NCBI nr
Match: XP_022995032.1 (subtilisin-like protease SBT4.3 [Cucurbita maxima])

HSP 1 Score: 580.5 bits (1495), Expect = 1.7e-161
Identity = 362/741 (48.85%), Postives = 444/741 (59.92%), Query Frame = 0


Query: 4   NRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSVVG 63
           N PILF  +AALLAA+ +S     A   +RK HIVYMG+++N AMA+S+HHLNLL+SV+G
Sbjct: 5   NFPILFSLIAALLAAIFTS-----ANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIG 64

Query: 64  TSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGF 123
           TSS TEG Y+RSYGRSFNGF A+LT +E E+LA M+GVVSVF SK LKTQTTRSWD++GF
Sbjct: 65  TSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLATMDGVVSVFESKELKTQTTRSWDFLGF 124

Query: 124 RRKIKRDVAGETDVIIGSIDTGIWSESESFNDD--------------------------- 183
           ++  KR++AGE DVIIGSID+GIW ESESFND+                           
Sbjct: 125 QQP-KRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCNHKVIG 184

Query: 184 --------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDSGC 243
                   ARD  GHG+HTASTA  ++V    FYGIA GVARGAVPS+R+A Y  C   C
Sbjct: 185 ARYYSTSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSC 244

Query: 244 HETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGP 303
            + ++LAAFDDAIADGVD+ITIS+ G   +  + D IAIG++HAM RGILTV SAG+DGP
Sbjct: 245 TDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVRGILTVQSAGNDGP 304

Query: 304 AEGSVGSVAPWLFL------------STPL--GIVLLGLQHSELLPSKQDET--SIDLRN 363
             GSV SVAPWLF              T L  G ++ G   +   P+K        +   
Sbjct: 305 VVGSVESVAPWLFSVAATTTDRSIIDETVLGNGKIVSGYSINPFTPNKNVTLIYGTNASK 364

Query: 364 KC-FKNLRFCS-----------KSCSVLRPWPQLLDAGDHEA------------------ 423
            C F+N   CS             C       + +DAG   A                  
Sbjct: 365 SCSFQNPEVCSCLDPKLVKGKIVQCKSFTGIFKAVDAGAAGAIVLNDKADNVSFIVPLPA 424

Query: 424 --------------------GHIRPRR----RNSLRLFTCSFSFRCS-------WRPKIC 483
                                H+R  +    ++        FS R          +P I 
Sbjct: 425 IALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPDIMKPDIA 484

Query: 484 ----QVLYNV---------------WDFHVL-------PHVAGVAAYVKNFHQNWSPSAI 543
               ++L +V                +F ++       PHVAG+AAYVK+FH NWSPSAI
Sbjct: 485 APGVEILASVNPQPPQSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSAI 544

Query: 544 KSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYDSK 603
           KSAIMTTA+++ +TDG   GE L+GSGQINPKQAI+PGLVYE  E DY+  L GNG+DSK
Sbjct: 545 KSAIMTTAEEMTNTDGFVSGEFLHGSGQINPKQAIEPGLVYEIFEVDYLKFLCGNGFDSK 604

Query: 604 AIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPTQPFVVKFQRTVTNVGVANSTY-SR 606
           ++  ISG+ S CS  +T F P+DLNYPAMV +VSP +PFV+KFQRTVTNVG+ANSTY S 
Sbjct: 605 SVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVSPMKPFVIKFQRTVTNVGIANSTYKSE 664

[Close]

BLAST of Sgr023230 vs. NCBI nr
Match: XP_023533940.1 (subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 577.8 bits (1488), Expect = 1.1e-160
Identity = 359/743 (48.32%), Postives = 446/743 (60.03%), Query Frame = 0


Query: 2   AKNRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSV 61
           A N PI F F+AALLAA+ +S     A   +RK HIVYMG+++N AMA+S+HHLNLLQSV
Sbjct: 3   ANNFPISFSFIAALLAAIFTS-----ANGSERKDHIVYMGAIQNRAMAESTHHLNLLQSV 62

Query: 62  VGTSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYV 121
           +GTSS TE  Y+RSYGRSFNGF AELT  E E+LAAM+GVVSVF SK LKT+TTRSWD++
Sbjct: 63  IGTSSVTEDSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFL 122

Query: 122 GFRRKIKRDVAGETDVIIGSIDTGIWSESESFNDD------------------------- 181
           GF++  KR++AGE DVIIGSID+GIW ESESFND+                         
Sbjct: 123 GFQQP-KRNLAGELDVIIGSIDSGIWPESESFNDEGMGPPPQKWRGECAGGENFTCNHKV 182

Query: 182 ----------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDS 241
                     ARD  GHG+HTASTA  ++V    FYGIA GVARGAVPS+R+A Y  C  
Sbjct: 183 IGARYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSP 242

Query: 242 GCHETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSD 301
            C + ++LAAFDDAIADGVD+ITIS+ G   +  + D IAIG++HAM +GILTV SAG+D
Sbjct: 243 SCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGND 302

Query: 302 GPAEGSVGSVAPWLF----LSTPLGIV----------LLGLQHSELLPSKQDET--SIDL 361
           GP  GSV SVAPWLF     +T   IV          + G   +   P+K        + 
Sbjct: 303 GPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNA 362

Query: 362 RNKC-FKNLRFCS--------------------------------------KSCSVLRPW 421
              C F+N   CS                                       + S + P 
Sbjct: 363 SKSCSFQNPEVCSCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPL 422

Query: 422 PQL---LDAGDHEAGHIRPRRRNSLRL------------FTCSFSFRCS-------WRPK 481
           P +   L A +  A +        +R+                FS R          +P 
Sbjct: 423 PAIALNLTAYEQVANYAISEPNPHVRILQSVAVKDGYAPMAAQFSSRGPNLLLPEIMKPD 482

Query: 482 IC----QVLYNV---------------WDFHVL-------PHVAGVAAYVKNFHQNWSPS 541
           I     ++L +V                +F ++       PHVAG+AAYVK+FH NWSPS
Sbjct: 483 IAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPS 542

Query: 542 AIKSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYD 601
           AIKSAIMTTA+++ +TDG   GE L+GSGQI+PKQAI+PGLVYE  E+DY+  L GNG+D
Sbjct: 543 AIKSAIMTTAEEMTNTDGFLSGEFLHGSGQIDPKQAIEPGLVYEIFEDDYLKFLCGNGFD 602

Query: 602 SKAIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPTQPFVVKFQRTVTNVGVANSTY- 606
           SK++  ISG+ S CS  +T F P+DLNYPAMV +V P +PF VKFQRTVTNVG+ANSTY 
Sbjct: 603 SKSVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFAVKFQRTVTNVGIANSTYK 662

[Close]

BLAST of Sgr023230 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.5e-112
Identity = 284/704 (40.34%), Postives = 362/704 (51.42%), Query Frame = 0


Query: 35  VHIVYMGSLRNMAMAQSSHHLNLLQSVVGTSSETEGLYVRSYGRSFNGFAAELTDSEREK 94
           V+IVYMG+L  +  +  SHHL++LQ +VGT + +  L VRSY RSFNGFAA L+ +E +K
Sbjct: 32  VYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASH-LLVRSYKRSFNGFAANLSQAESQK 91

Query: 95  LAAMEGVVSVFPSKILKTQTTRSWDYVGFRRKIKRDVAGETDVIIGSIDTGIWSESESFN 154
           L  M+ VVSVFPSK  +  TTRSWD+VGF  K +R+   E+DVI+G ID+GIW ESESF+
Sbjct: 92  LQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFD 151

Query: 155 DD-------------------------------------ARDSIGHGSHTASTAARRAVK 214
           D+                                     ARD  GHG+HTASTAA  AV+
Sbjct: 152 DEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQ 211

Query: 215 HASFYGIAAGVARGAVPSARIAAYKVCDSGCHETDVLAAFDDAIADGVDLITISISGVCA 274
            ASFYG+A G ARG VPSARIAAYKVC + C++ D+LAAFDDAIADGVD+I+ISIS    
Sbjct: 212 AASFYGLAQGTARGGVPSARIAAYKVCFNRCNDVDILAAFDDAIADGVDVISISISADYV 271

Query: 275 TPFKYDGIAIGSFHAMARGILTVHSAGSDGPAEGSVGSVAPWLFLSTPLGI-------VL 334
           +      +AIGSFHAM RGI+T  SAG++GP +GSV +V+PW+      G        V+
Sbjct: 272 SNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVV 331

Query: 335 LG----LQHSELLPSKQDETSIDL-------RNKCFKNLRFCSKSC-------------- 394
           LG    L    +     + T   +       RN       +CS  C              
Sbjct: 332 LGNGKALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCD 391

Query: 395 --------------------------SVLRPWP-QLLDAGDHEA-----GHIRPRRRNSL 454
                                     + + P+P   L   D+++         P +   L
Sbjct: 392 DFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEIL 451

Query: 455 RL---------FTCSFSFR----------------------CSWRPKIC----------- 514
           R          +  SFS R                       ++ P              
Sbjct: 452 RTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKR 511

Query: 515 QVLYNVWD--FHVLPHVAGVAAYVKNFHQNWSPSAIKSAIMTTAKQIISTDGMHIGECLY 574
            V Y+V        PHVAGVAAYVK+FH +WSPSAIKSAIMTTA  +         E  Y
Sbjct: 512 SVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE-QEFAY 571

Query: 575 GSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYDSKAIELISGDNSTCSTNATTFPPKDL 594
           GSGQINP +A  PGLVYE   EDY+ +L   G+DS  +   SG N TCS        KDL
Sbjct: 572 GSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTEV---KDL 631

[Close]

BLAST of Sgr023230 vs. ExPASy Swiss-Prot
Match: Q9FIM6 (Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.0e-108
Identity = 289/718 (40.25%), Postives = 378/718 (52.65%), Query Frame = 0


Query: 9   FQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMA-MAQSSHHLNLLQSVVGTSSE 68
           F  ++ L+    SS         D++V++VYMGSL +       S+H+N+LQ V G SS 
Sbjct: 7   FCLLSCLIILFLSSVSAIIYDPQDKQVYVVYMGSLPSQPNYTPMSNHINILQEVTGESS- 66

Query: 69  TEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFR--R 128
            EG  VRSY RSFNGF+A LT+SERE +A MEGVVSVF SK  K QTT SWD++G +  +
Sbjct: 67  IEGRLVRSYKRSFNGFSALLTESEREGVAEMEGVVSVFRSKNYKLQTTASWDFMGMKEGK 126

Query: 129 KIKRDVAGETDVIIGSIDTGIWSESESFND------------------------------ 188
             KR+ A E+D IIG ID+GIW ESESF+D                              
Sbjct: 127 NTKRNFAVESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCKGGKNFTCNNKLIGAR 186

Query: 189 -----DARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCD-SGCH 248
                  RD  GHG+HT STAA  AV   SF+GI  G ARG VP++R+AAYKVC  +GC 
Sbjct: 187 DYTSEGTRDLQGHGTHTTSTAAGNAVADTSFFGIGNGTARGGVPASRVAAYKVCTITGCS 246

Query: 249 ETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGPA 308
           + +VL+AFDDAIADGVDLI++S+ G   + +  D IAIG+FHAMA+GILTVHSAG+ GP 
Sbjct: 247 DDNVLSAFDDAIADGVDLISVSLGGDYPSLYAEDTIAIGAFHAMAKGILTVHSAGNAGPN 306

Query: 309 EGSVGSVAPWL-----------FLSTPL---GIVLLG--------------LQHSELL-- 368
             +V SVAPW+           FL+  +   G  L+G              L++ + L  
Sbjct: 307 PTTVVSVAPWMLTVAATTTNRRFLTKVVLGNGKTLVGKSVNAFDLKGKKYPLEYGDYLNE 366

Query: 369 --------------PSKQDETSIDLRNKCFKNLRFCSKSCSVLRPWPQLLDAGDHEAGHI 428
                          S+   + I   NK + ++   S+  SVL       D  D    +I
Sbjct: 367 SLVKGKILVSRYLSGSEVAVSFITTDNKDYASI--SSRPLSVLSQ-----DDFDSLVSYI 426

Query: 429 RPRRR------------NSLRLFTCSFSFRCS-------WRPKIC--------------- 488
              R             N L     SFS R          +P I                
Sbjct: 427 NSTRSPQGSVLKTEAIFNQLSPKVASFSSRGPNTIAVDILKPDISAPGVEILAAYSPLSL 486

Query: 489 ---------QVLYNVWD--FHVLPHVAGVAAYVKNFHQNWSPSAIKSAIMTTAKQIIST- 548
                    +V Y+V        PHV GVAAY+K FH +WSPS I+SAIMTTA Q+ +T 
Sbjct: 487 PSEDRRDKRRVKYSVLSGTSMACPHVTGVAAYIKTFHPDWSPSVIQSAIMTTAWQMNATG 546

Query: 549 DGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYDSKAIELISGDNSTCST 597
            G    E  YG+G ++P  AI PGLVYE ++ D+++ L G  Y SK ++LISGD   CS 
Sbjct: 547 TGAESTEFAYGAGHVDPIAAINPGLVYELNKTDHISFLCGMNYTSKTLKLISGDAVICSG 606

[Close]

BLAST of Sgr023230 vs. ExPASy Swiss-Prot
Match: Q9FIG1 (Subtilisin-like protease SBT4.11 OS=Arabidopsis thaliana OX=3702 GN=SBT4.11 PE=2 SV=2)

HSP 1 Score: 395.2 bits (1014), Expect = 1.3e-108
Identity = 281/715 (39.30%), Postives = 368/715 (51.47%), Query Frame = 0


Query: 32  DRKVHIVYMGSLRNMA-MAQSSHHLNLLQSVVGTSSETEGLYVRSYGRSFNGFAAELTDS 91
           D++V+IVYMGSL + A     SHH+N+LQ V   SS  EG  VRSY RSFNGF A LT+S
Sbjct: 32  DKQVYIVYMGSLPSRADYTPMSHHMNILQEVARESS-IEGRLVRSYKRSFNGFVARLTES 91

Query: 92  EREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFR--RKIKRDVAGETDVIIGSIDTGIWS 151
           ERE++A MEGVVSVFP+K LK QT+ SWD++G +  +  KR+ + E+D IIG  D GIW 
Sbjct: 92  ERERVADMEGVVSVFPNKKLKLQTSASWDFMGLKEGKGTKRNPSVESDTIIGVFDGGIWP 151

Query: 152 ESESFND-----------------------------------DARDSIGHGSHTASTAAR 211
           ESESF+D                                   DARDS GHG+HTAS AA 
Sbjct: 152 ESESFSDKGFGPPPKKWKGICAGGKNFTCNNKLIGARHYSPGDARDSTGHGTHTASIAAG 211

Query: 212 RAVKHASFYGIAAGVARGAVPSARIAAYKVCDSGCHETDVLAAFDDAIADGVDLITISIS 271
            AV + SF+GI  G  RGAVP++RIA Y+VC   C +  +L+AFDDAI+DGVD+ITISI 
Sbjct: 212 NAVANTSFFGIGNGTVRGAVPASRIAVYRVCAGECRDDAILSAFDDAISDGVDIITISIG 271

Query: 272 GVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGPAEGSVGSVAPWL-----------FL 331
            +   PF+ D IAIG+FHAM++GILTV++AG+ GP   S+ S+APWL           F+
Sbjct: 272 DINVYPFEKDPIAIGAFHAMSKGILTVNAAGNTGPDTASITSLAPWLLTVAASTANREFV 331

Query: 332 STPL---GIVLLGLQ----------------HSELLPSKQDETSIDLRNKCFK------N 391
           S  +   G  L+G                   S  L   Q + + D   +C         
Sbjct: 332 SKVVLGDGKTLVGKSVNGFDLKGKKFPLVYGKSAALSLSQAKCAEDCTPECLDASLVKGK 391

Query: 392 LRFCSKSCSVL--------------RPWPQL-------LDAGDHE--------------- 451
           +  C++    +                W Q+       L   D E               
Sbjct: 392 ILVCNRFLPYVAYTKRAVAAIFEDGSDWAQINGLPVSGLQKDDFESVLSYFKSEKSPEAA 451

Query: 452 ---------------------------AGHIRPRRRNSLRLFTCSFSFRCSWRPKICQVL 511
                                      A  ++P           + S R S       V 
Sbjct: 452 VLKSESIFYQTAPKILSFSSRGPNIIVADILKPDITAPGLEILAANSLRASPFYDTAYVK 511

Query: 512 YNVWDFHVL--PHVAGVAAYVKNFHQNWSPSAIKSAIMTTAKQI-ISTDGMHIGECLYGS 571
           Y+V     +  PH AGVAAYVK FH  WSPS IKSAIMTTA  +  S  G    E  YG+
Sbjct: 512 YSVESGTSMSCPHAAGVAAYVKTFHPQWSPSMIKSAIMTTAWSMNASQSGYASTEFAYGA 571

Query: 572 GQINPKQAIKPGLVYETSEEDYVNLLRGNGYDSKAIELISGDNSTCSTNATTFPPKDLNY 606
           G ++P  A  PGLVYE ++ DY   L G  Y+   ++LISG+  TCS   +   P++LNY
Sbjct: 572 GHVDPIAATNPGLVYEITKTDYFAFLCGMNYNKTTVKLISGEAVTCSEKIS---PRNLNY 631

[Close]

BLAST of Sgr023230 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.7e-108
Identity = 284/739 (38.43%), Postives = 374/739 (50.61%), Query Frame = 0


Query: 7   ILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSL-RNMAMAQSSHHLNLLQSVVGTS 66
           + +  ++ + A +  S   +   + D++ +IVYMG+L   +     SHH ++LQ V G S
Sbjct: 6   VSYCLLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGES 65

Query: 67  SETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFR- 126
           S  E   VR+Y RSFNGFAA LT SERE LA+M+ VVSVFP+K LK QTT SW+++G + 
Sbjct: 66  S-IEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKE 125

Query: 127 -RKIKRDVAGETDVIIGSIDTGIWSESESFN----------------------------- 186
            ++ KR+   E+D IIG ID+GI+ ES+SF+                             
Sbjct: 126 SKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIG 185

Query: 187 ------------DDARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYK 246
                       + ARD +GHGSHTASTAA  AVKH SFYG+  G ARG VP+ARIA YK
Sbjct: 186 ARYYTPKLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYK 245

Query: 247 VCD---SGCHETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILT 306
           VCD    GC    +LAAFDDAIAD VD+ITISI G  ++PF+ D IAIG+FHAMA+GIL 
Sbjct: 246 VCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILI 305

Query: 307 VHSAGSDGPAEGSVGSVAPWLFLSTPLGIVLLGLQHSELLPSK---QDETSIDLRNKCF- 366
           V+SAG+ GP   +V S+APW+F           +    L   K   +   S DL  K + 
Sbjct: 306 VNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTVGRSVNSFDLNGKKYP 365

Query: 367 -------------KNLRFCSKSC---------SVLRPWPQLLD---AGDHEAGHIRPRRR 426
                         +  FCS  C          VL   PQ  D   A    A  +R  R 
Sbjct: 366 LVYGKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHRT 425

Query: 427 NSLRLFTCSFS-------------FRCSWRPKIC-------------------------- 486
           +   +F+   S                +  PK                            
Sbjct: 426 DVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTI 485

Query: 487 --------------------------------QVLYNV--WDFHVLPHVAGVAAYVKNFH 546
                                           +V Y+V        PHVAGVAAY+K+FH
Sbjct: 486 IPDILKPDITAPGSEIVAAYSPDAPPSISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 545

Query: 547 QNWSPSAIKSAIMTTAKQIISTDG--MHIGECLYGSGQINPKQAIKPGLVYETSEEDYVN 595
             WSPS I+SAIMTTA  + ++      + E  YG+G ++P  AI PGLVYE ++ D++ 
Sbjct: 546 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 605

[Close]

BLAST of Sgr023230 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.9e-107
Identity = 284/728 (39.01%), Postives = 379/728 (52.06%), Query Frame = 0


Query: 15  LLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMA-MAQSSHHLNLLQSVVGTSSETEGLYV 74
           LL  + SS   SA I+ D +V+IVYMGSL + A    +S H+++LQ V G SS  EG  V
Sbjct: 14  LLVLLLSSV--SAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESS-IEGRLV 73

Query: 75  RSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFR--RKIKRDV 134
           RSY RSFNGFAA LT+SER  +A +EGVVSVFP+KIL+  TT SWD++G +  +  KR++
Sbjct: 74  RSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNL 133

Query: 135 AGETDVIIGSIDTGIWSESESFND-----------------------------------D 194
           A E+D IIG IDTGIW ES+SF+D                                    
Sbjct: 134 AIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEG 193

Query: 195 ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVC-DSGCHETDVLA 254
            RD+ GHG+HTASTAA  AVK  SF+GI  G  RG VP++RIAAYKVC DSGC    +L+
Sbjct: 194 TRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLS 253

Query: 255 AFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGPAEGSVGS 314
           +FDDAIADGVDLITISI     + F+ D IAIG+FHAMA+GILTV SAG+ GP   +V  
Sbjct: 254 SFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSH 313

Query: 315 VAPWLFL----STPLGIVLLGLQHSELLPSKQDETSIDLRNKCF---------------K 374
           VAPW+F     +T  G +   +  +    + +   + D++ K +               K
Sbjct: 314 VAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAK 373

Query: 375 NLRFCSKSC--------SVL----------------------RPWPQLLDAGDHEAGHIR 434
               C+ +C         +L                       P P +       A  ++
Sbjct: 374 TAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLK 433

Query: 435 PRRRNSLRLF------------------------TCSFSFRCS-------WRPKIC---- 494
            +   SL  +                          SFS R          +P I     
Sbjct: 434 AKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGV 493

Query: 495 ------------------QVLYNVWD--FHVLPHVAGVAAYVKNFHQNWSPSAIKSAIMT 554
                             +V Y+V+       PHVAGVAAYVK F+  WSPS I+SAIMT
Sbjct: 494 EILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMT 553

Query: 555 TAKQIISTD-GMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYDSKAIELI 598
           TA  + +   G+   E  YG+G ++P  A+ PGLVYE  + D++  L G  Y SK +++I
Sbjct: 554 TAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKII 613

[Close]

BLAST of Sgr023230 vs. ExPASy TrEMBL
Match: A0A6J1K0V8 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 2.0e-163
Identity = 359/744 (48.25%), Postives = 444/744 (59.68%), Query Frame = 0


Query: 4   NRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSVVG 63
           N PILF F+AALLAA+ SS     A   +RK HIVYMG+++N AMA+S+HHLNLL+SV+G
Sbjct: 5   NFPILFSFIAALLAAILSS-----ANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIG 64

Query: 64  TSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGF 123
           TSS TEG Y+RSYGRSFNGF A+LT +E E+LAAM+GVVSVF SK+ KTQTTRSWDY+GF
Sbjct: 65  TSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGF 124

Query: 124 RRKIKRDVAGETDVIIGSIDTGIWSESESFNDD--------------------------- 183
             K  R++AGETDVIIGSIDTGIW E ESFND+                           
Sbjct: 125 PAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIG 184

Query: 184 --------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDSGC 243
                   ARD++GHGSHTASTAA +  K   FYG+A G ARGAVPS+R+A YKVC+  C
Sbjct: 185 ARFYTSMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPDC 244

Query: 244 HETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGP 303
            E ++LAAFDDAIADGVDLITISI GV    F++D +AIGS+H+MA+GILTV SAG+ GP
Sbjct: 245 LEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGP 304

Query: 304 AEGSVGSVAPWLFLSTPL--------------GIVLLGLQHSELLPSKQDE---TSIDLR 363
            +G+VGSV PW+F                   G  + G   +   P++       +   R
Sbjct: 305 VDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASR 364

Query: 364 NKCFKNLRFCSKSC----------------------------------------SVLRPW 423
           N   KN   CS  C                                        S + P+
Sbjct: 365 NCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPF 424

Query: 424 PQL-LDAGDHE--AGHIRPRRRNSLRLFTCSFSFRCSWRPKICQVLY---NVWDFHVL-- 483
           P + L   D++  A +       ++ +F  S + +  + P I        N+W   +L  
Sbjct: 425 PAIALKMADYKSVANYAISATNPNVTIFR-SVATKDPYAPTIADFSSRGPNMWMLEILKP 484

Query: 484 -----------------------------------------PHVAGVAAYVKNFHQNWSP 543
                                                    PHVAGVAAYVK+FH NWSP
Sbjct: 485 DIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSP 544

Query: 544 SAIKSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGY 603
           +AIKSAIMTTAKQI+ TDG+ I E LYGSG I+P +AI+PGLVYE  E+D++N+L   GY
Sbjct: 545 AAIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGY 604

Query: 604 DSKAIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPTQPFVVKFQRTVTNVGVANSTY 606
           DSK +E  +G++S CS   T F  +DLNYPAMVA V P +PFVVKFQRTVTNVGVANSTY
Sbjct: 605 DSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTY 664

[Close]

BLAST of Sgr023230 vs. ExPASy TrEMBL
Match: A0A6J1H1M3 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 5.7e-163
Identity = 365/743 (49.13%), Postives = 446/743 (60.03%), Query Frame = 0


Query: 2   AKNRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSV 61
           A N PILF F+AALLAA+ +S     A   +RK HIVYMG+++N AMA+S+HHLNLL+SV
Sbjct: 3   AINFPILFTFIAALLAAIFTS-----ANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSV 62

Query: 62  VGTSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYV 121
           +GTSS TEG Y+RSYGRSFNGF AELT  E E+LAAM+GVVSVF SK LKT+TTRSWD++
Sbjct: 63  IGTSSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFL 122

Query: 122 GFRRKIKRDVAGETDVIIGSIDTGIWSESESFNDD------------------------- 181
           GF++  KR+++GE DVIIGSID+GIW ESESFNDD                         
Sbjct: 123 GFQQP-KRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCNHKV 182

Query: 182 ----------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDS 241
                     ARD  GHG+HTASTA  ++V    FYGIA GVARGAVPS+R+A Y  C  
Sbjct: 183 IGARYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSP 242

Query: 242 GCHETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSD 301
            C + ++LAAFDDAIADGVD+ITIS+ G   +  + D IAIG++HAM +GILTV SAG+D
Sbjct: 243 SCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGND 302

Query: 302 GPAEGSVGSVAPWLF----LSTPLGIV----------LLGLQHSELLPSKQDET--SIDL 361
           GP  GSV SVAPWLF     +T   IV          + G   +   P+K        + 
Sbjct: 303 GPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNA 362

Query: 362 RNKC-FKNLRFCS-----------KSCSVLRPWPQLLDAGDHEA---------------- 421
              C F+N   CS             C       + +DAG   A                
Sbjct: 363 SKSCSFQNPEVCSCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPL 422

Query: 422 ----------------------GHIRPRR----RNSLRLFTCSFSFRCS-------WRPK 481
                                  H+R  +    ++        FS R          +P 
Sbjct: 423 PATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPEIMKPD 482

Query: 482 IC----QVLYNV---------------WDFHVL-------PHVAGVAAYVKNFHQNWSPS 541
           I     ++L +V                +F ++       PHVAG+AAYVK+FH NWSPS
Sbjct: 483 IAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPS 542

Query: 542 AIKSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYD 601
           AIKSAIMTTA++I +TDG   GE L+GSGQINPKQAI+PGLVYE  E+DY+  L GNG+D
Sbjct: 543 AIKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKFLCGNGFD 602

Query: 602 SKAIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPTQPFVVKFQRTVTNVGVANSTY- 606
           SK++  ISG+ S CS  +T F P+DLNYPAMV +V P +PFVVKFQRTVTNVG ANSTY 
Sbjct: 603 SKSVRGISGNKSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNVGTANSTYK 662

[Close]

BLAST of Sgr023230 vs. ExPASy TrEMBL
Match: A0A6J1JXM0 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 8.2e-162
Identity = 362/741 (48.85%), Postives = 444/741 (59.92%), Query Frame = 0


Query: 4   NRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSVVG 63
           N PILF  +AALLAA+ +S     A   +RK HIVYMG+++N AMA+S+HHLNLL+SV+G
Sbjct: 5   NFPILFSLIAALLAAIFTS-----ANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIG 64

Query: 64  TSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGF 123
           TSS TEG Y+RSYGRSFNGF A+LT +E E+LA M+GVVSVF SK LKTQTTRSWD++GF
Sbjct: 65  TSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLATMDGVVSVFESKELKTQTTRSWDFLGF 124

Query: 124 RRKIKRDVAGETDVIIGSIDTGIWSESESFNDD--------------------------- 183
           ++  KR++AGE DVIIGSID+GIW ESESFND+                           
Sbjct: 125 QQP-KRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCNHKVIG 184

Query: 184 --------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDSGC 243
                   ARD  GHG+HTASTA  ++V    FYGIA GVARGAVPS+R+A Y  C   C
Sbjct: 185 ARYYSTSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSC 244

Query: 244 HETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGP 303
            + ++LAAFDDAIADGVD+ITIS+ G   +  + D IAIG++HAM RGILTV SAG+DGP
Sbjct: 245 TDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVRGILTVQSAGNDGP 304

Query: 304 AEGSVGSVAPWLFL------------STPL--GIVLLGLQHSELLPSKQDET--SIDLRN 363
             GSV SVAPWLF              T L  G ++ G   +   P+K        +   
Sbjct: 305 VVGSVESVAPWLFSVAATTTDRSIIDETVLGNGKIVSGYSINPFTPNKNVTLIYGTNASK 364

Query: 364 KC-FKNLRFCS-----------KSCSVLRPWPQLLDAGDHEA------------------ 423
            C F+N   CS             C       + +DAG   A                  
Sbjct: 365 SCSFQNPEVCSCLDPKLVKGKIVQCKSFTGIFKAVDAGAAGAIVLNDKADNVSFIVPLPA 424

Query: 424 --------------------GHIRPRR----RNSLRLFTCSFSFRCS-------WRPKIC 483
                                H+R  +    ++        FS R          +P I 
Sbjct: 425 IALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPDIMKPDIA 484

Query: 484 ----QVLYNV---------------WDFHVL-------PHVAGVAAYVKNFHQNWSPSAI 543
               ++L +V                +F ++       PHVAG+AAYVK+FH NWSPSAI
Sbjct: 485 APGVEILASVNPQPPQSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSAI 544

Query: 544 KSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYDSK 603
           KSAIMTTA+++ +TDG   GE L+GSGQINPKQAI+PGLVYE  E DY+  L GNG+DSK
Sbjct: 545 KSAIMTTAEEMTNTDGFVSGEFLHGSGQINPKQAIEPGLVYEIFEVDYLKFLCGNGFDSK 604

Query: 604 AIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPTQPFVVKFQRTVTNVGVANSTY-SR 606
           ++  ISG+ S CS  +T F P+DLNYPAMV +VSP +PFV+KFQRTVTNVG+ANSTY S 
Sbjct: 605 SVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVSPMKPFVIKFQRTVTNVGIANSTYKSE 664

[Close]

BLAST of Sgr023230 vs. ExPASy TrEMBL
Match: A0A6J1H1W0 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 4.7e-157
Identity = 351/736 (47.69%), Postives = 438/736 (59.51%), Query Frame = 0


Query: 3   KNRPILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSVV 62
           +N PILF F+AALLAA+ +S     A   +RK HIVYMG+++N AMA+S+HHLNLL+SV+
Sbjct: 4   ENFPILFSFIAALLAAIFTS-----ANGSERKAHIVYMGAVQNRAMAESTHHLNLLRSVI 63

Query: 63  GTSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVG 122
           GTSS  EG Y+RSYGRSFNGF A+LT  E E+LAAM+GVVSVF SK+ KTQTTRSWDY+G
Sbjct: 64  GTSSVMEGSYIRSYGRSFNGFVAKLTGGEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLG 123

Query: 123 FRRKIKRDVAGETDVIIGSIDTGIWSESESFNDD-------------------------- 182
           F  K  R++AGETDVIIGSIDTGIW E ESFND+                          
Sbjct: 124 FPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCTGGGNFTCNNKVI 183

Query: 183 ---------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVCDSG 242
                    ARD++GHGSHTASTAA ++ K   FYG+A GVARGAVPS+R+A YK C+  
Sbjct: 184 GARFYTSMSARDNVGHGSHTASTAAGKSGKTTGFYGLAGGVARGAVPSSRLAIYKACNPD 243

Query: 243 CHETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDG 302
           C E ++LAAFDDAIADGVDLITISI G     F++D +AIGS+H+MA+GILTV SAG+ G
Sbjct: 244 CLEENILAAFDDAIADGVDLITISIVGAGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYG 303

Query: 303 PAEGSVGSVAPWLFLSTPL--------------GIVLLGLQHSELLPSKQDE---TSIDL 362
           P +G+VGSV PW+F                   G  + G   +   P++       +   
Sbjct: 304 PEDGTVGSVVPWVFTVAATNTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNAS 363

Query: 363 RNKCFKNLRFCSKSC----------------------------------------SVLRP 422
           RN   KN   C   C                                        S + P
Sbjct: 364 RNCSSKNAEICRDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLP 423

Query: 423 WPQL-LDAGDHE--AGHIRPRRRNSLRLF------------TCSFSFR------------ 482
           +P + L   D++  A +       ++ +F               FS R            
Sbjct: 424 FPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPHAPMIADFSSRGPNMWMLEILKP 483

Query: 483 ----------CSWRPKICQVLYNVWD-----FHVL-------PHVAGVAAYVKNFHQNWS 542
                      S+ P I     NV D     F +L       PHVAGVAAYVK+FH NWS
Sbjct: 484 DIAAPGVEILASFSP-IAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWS 543

Query: 543 PSAIKSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNG 597
           P+AIKSAIMTTAKQI+ TDG+ I E LYGSG ++P +AI+PGLVYE  E+D++N+L   G
Sbjct: 544 PAAIKSAIMTTAKQIVRTDGILIREFLYGSGLVDPDRAIEPGLVYEIFEKDHLNMLCAKG 603

[Close]

BLAST of Sgr023230 vs. ExPASy TrEMBL
Match: A0A5C7IY30 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_002770 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 2.0e-128
Identity = 313/744 (42.07%), Postives = 396/744 (53.23%), Query Frame = 0


Query: 7   ILFQFMAALLAAVCSSTLCSAA-INGDRKVHIVYMGSLRNMAMAQSSHHLNLLQSVVGTS 66
           +LFQ+ + LL   C + LC A   + DR +HIVYMGSL+    + +S HL +LQ V+G+S
Sbjct: 7   LLFQYCSFLL---CMNILCGATDADTDRNLHIVYMGSLQEGQYSPASQHLTMLQEVIGSS 66

Query: 67  SETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFRR 126
           S  E + VRSY RSFNGFAA+LTD+ER+KL++M+GVVS+FPS  L  QTTRSWD++GF  
Sbjct: 67  S-LENVLVRSYTRSFNGFAAKLTDNERQKLSSMDGVVSIFPSGTLHLQTTRSWDFMGFNE 126

Query: 127 KIKRDVAGETDVIIGSIDTGIWSESESFNDD----------------------------- 186
            + R    E+++I+G ID+GIW ES+SF+D+                             
Sbjct: 127 SVSRKRKTESNIIVGVIDSGIWPESDSFSDEGFGPPPSKWKGACRGGKNFTCNNKIIGAR 186

Query: 187 ----------ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVC-- 246
                     ARD  GHGSHTASTAA   VK ASFYG+A G ARG VPSARIAAY+VC  
Sbjct: 187 YYNSASSEISARDEQGHGSHTASTAAGNQVKDASFYGLAKGTARGGVPSARIAAYRVCYP 246

Query: 247 DSGCHETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAG 306
           D+GC + D+LAAFDDAIADGVDLITISI G  A  F  D IAIG+FHAMA+GILTV +AG
Sbjct: 247 DTGCADADILAAFDDAIADGVDLITISIGGSNAIEFNEDVIAIGAFHAMAKGILTVQAAG 306

Query: 307 SDGPAEGSVGSVAPWLFL-------------------STPLGIVL---------LGLQHS 366
           +DGP   SV SVAPWLF                     T  GI +         L L H 
Sbjct: 307 NDGPKSSSVSSVAPWLFSVAASSTDRMIVDNIILGNGKTVQGISINSFSLDGQKLPLVHG 366

Query: 367 ELLPSKQDETSIDLRNKCFK----------NLRFCSKS---CSVLRPWPQ----LLDAGD 426
           + + S  D+ S      CF            +  C +       LR        L D   
Sbjct: 367 KEVLSLTDDCSTTSAENCFPGCLDSRKVKGKIIICDREMGYVGALRAGAAGSILLTDLSK 426

Query: 427 HEAGHIR-----PRRRNSLRLFTCSFSFRCSWRPKICQVLYN------------------ 486
            +A  +      P    S + +    S++ S +  + ++L +                  
Sbjct: 427 EDAFLVAFIVPLPASAVSSKEYNAILSYKNSTKNPLAEILKSEAIKNTTAPVVTKFSSRG 486

Query: 487 --------------------------------------VWDFHVL-------PHVAGVAA 546
                                                    +H++       PHV GVAA
Sbjct: 487 PNSLVPEILKPDISAPGLQILAAYSPIAPISEYLDDKRSVKYHIISGTSMSCPHVTGVAA 546

Query: 547 YVKNFHQNWSPSAIKSAIMTTAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEE 595
           YVK FH +WSPSAIKSAIMTTA  +  +     GE  YGSG INP +AI PGLVYET + 
Sbjct: 547 YVKTFHPDWSPSAIKSAIMTTAWTMDPSKNGD-GEFAYGSGHINPLEAINPGLVYETLKP 606

[Close]

BLAST of Sgr023230 vs. TAIR 10
Match: AT5G59130.2 (Subtilase family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 7.7e-112
Identity = 284/705 (40.28%), Postives = 372/705 (52.77%), Query Frame = 0


Query: 32  DRKVHIVYMGSLRNMA-MAQSSHHLNLLQSVVGTSSETEGLYVRSYGRSFNGFAAELTDS 91
           D++V+IVYMGSL + A     SHH+N+LQ V   SS  EG  VRSY RSFNGF A LT+S
Sbjct: 32  DKQVYIVYMGSLPSRADYTPMSHHMNILQEVARESS-IEGRLVRSYKRSFNGFVARLTES 91

Query: 92  EREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFR--RKIKRDVAGETDVIIGSIDTGIWS 151
           ERE++A MEGVVSVFP+K LK QT+ SWD++G +  +  KR+ + E+D IIG  D GIW 
Sbjct: 92  ERERVADMEGVVSVFPNKKLKLQTSASWDFMGLKEGKGTKRNPSVESDTIIGVFDGGIWP 151

Query: 152 ESESFND-----------------------------------DARDSIGHGSHTASTAAR 211
           ESESF+D                                   DARDS GHG+HTAS AA 
Sbjct: 152 ESESFSDKGFGPPPKKWKGICAGGKNFTCNNKLIGARHYSPGDARDSTGHGTHTASIAAG 211

Query: 212 RAVKHASFYGIAAGVARGAVPSARIAAYKVCDSGCHETDVLAAFDDAIADGVDLITISIS 271
            AV + SF+GI  G  RGAVP++RIA Y+VC   C +  +L+AFDDAI+DGVD+ITISI 
Sbjct: 212 NAVANTSFFGIGNGTVRGAVPASRIAVYRVCAGECRDDAILSAFDDAISDGVDIITISIG 271

Query: 272 GVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGPAEGSVGSVAPWL-----------FL 331
            +   PF+ D IAIG+FHAM++GILTV++AG+ GP   S+ S+APWL           F+
Sbjct: 272 DINVYPFEKDPIAIGAFHAMSKGILTVNAAGNTGPDTASITSLAPWLLTVAASTANREFV 331

Query: 332 STPL---GIVLLGLQ----------------HSELLPSKQDETSIDLRNKCFK------N 391
           S  +   G  L+G                   S  L   Q + + D   +C         
Sbjct: 332 SKVVLGDGKTLVGKSVNGFDLKGKKFPLVYGKSAALSLSQAKCAEDCTPECLDASLVKGK 391

Query: 392 LRFCSKSCSVL--------------RPWPQL-------LDAGDHEAGHIRPRRRNSLRLF 451
           +  C++    +                W Q+       L   D E+      +  S+   
Sbjct: 392 ILVCNRFLPYVAYTKRAVAAIFEDGSDWAQINGLPVSGLQKDDFESPEAAVLKSESIFYQ 451

Query: 452 TC----SFSFRCS-------WRPKI---------------------CQVLYNVWDFHVL- 511
           T     SFS R          +P I                       V Y+V     + 
Sbjct: 452 TAPKILSFSSRGPNIIVADILKPDITAPGLEILAANSLRASPFYDTAYVKYSVESGTSMS 511

Query: 512 -PHVAGVAAYVKNFHQNWSPSAIKSAIMTTAKQI-ISTDGMHIGECLYGSGQINPKQAIK 571
            PH AGVAAYVK FH  WSPS IKSAIMTTA  +  S  G    E  YG+G ++P  A  
Sbjct: 512 CPHAAGVAAYVKTFHPQWSPSMIKSAIMTTAWSMNASQSGYASTEFAYGAGHVDPIAATN 571

Query: 572 PGLVYETSEEDYVNLLRGNGYDSKAIELISGDNSTCSTNATTFPPKDLNYPAMVAQVSPT 606
           PGLVYE ++ DY   L G  Y+   ++LISG+  TCS   +   P++LNYP+M A++S +
Sbjct: 572 PGLVYEITKTDYFAFLCGMNYNKTTVKLISGEAVTCSEKIS---PRNLNYPSMSAKLSGS 631

[Close]

BLAST of Sgr023230 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.9e-111
Identity = 280/699 (40.06%), Postives = 357/699 (51.07%), Query Frame = 0


Query: 40  MGSLRNMAMAQSSHHLNLLQSVVGTSSETEGLYVRSYGRSFNGFAAELTDSEREKLAAME 99
           MG+L  +  +  SHHL++LQ +VGT + +  L VRSY RSFNGFAA L+ +E +KL  M+
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASH-LLVRSYKRSFNGFAANLSQAESQKLQNMK 60

Query: 100 GVVSVFPSKILKTQTTRSWDYVGFRRKIKRDVAGETDVIIGSIDTGIWSESESFNDD--- 159
            VVSVFPSK  +  TTRSWD+VGF  K +R+   E+DVI+G ID+GIW ESESF+D+   
Sbjct: 61  EVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFG 120

Query: 160 ----------------------------------ARDSIGHGSHTASTAARRAVKHASFY 219
                                             ARD  GHG+HTASTAA  AV+ ASFY
Sbjct: 121 PPPKKWKGSCKGGLKFACNNKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFY 180

Query: 220 GIAAGVARGAVPSARIAAYKVCDSGCHETDVLAAFDDAIADGVDLITISISGVCATPFKY 279
           G+A G ARG VPSARIAAYKVC + C++ D+LAAFDDAIADGVD+I+ISIS    +    
Sbjct: 181 GLAQGTARGGVPSARIAAYKVCFNRCNDVDILAAFDDAIADGVDVISISISADYVSNLLN 240

Query: 280 DGIAIGSFHAMARGILTVHSAGSDGPAEGSVGSVAPWLFLSTPLGI-------VLLG--- 339
             +AIGSFHAM RGI+T  SAG++GP +GSV +V+PW+      G        V+LG   
Sbjct: 241 ASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGK 300

Query: 340 -LQHSELLPSKQDETSIDL-------RNKCFKNLRFCSKSC------------------- 399
            L    +     + T   +       RN       +CS  C                   
Sbjct: 301 ALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGY 360

Query: 400 ---------------------SVLRPWP-QLLDAGDHEA-----GHIRPRRRNSLRL--- 459
                                + + P+P   L   D+++         P +   LR    
Sbjct: 361 REAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEI 420

Query: 460 ------FTCSFSFR----------------------CSWRPKIC-----------QVLYN 519
                 +  SFS R                       ++ P               V Y+
Sbjct: 421 VDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYS 480

Query: 520 VWD--FHVLPHVAGVAAYVKNFHQNWSPSAIKSAIMTTAKQIISTDGMHIGECLYGSGQI 579
           V        PHVAGVAAYVK+FH +WSPSAIKSAIMTTA  +         E  YGSGQI
Sbjct: 481 VMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE-QEFAYGSGQI 540

Query: 580 NPKQAIKPGLVYETSEEDYVNLLRGNGYDSKAIELISGDNSTCSTNATTFPPKDLNYPAM 594
           NP +A  PGLVYE   EDY+ +L   G+DS  +   SG N TCS        KDLNYP M
Sbjct: 541 NPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTEV---KDLNYPTM 600

[Close]

BLAST of Sgr023230 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 394.8 bits (1013), Expect = 1.2e-109
Identity = 284/739 (38.43%), Postives = 374/739 (50.61%), Query Frame = 0


Query: 7   ILFQFMAALLAAVCSSTLCSAAINGDRKVHIVYMGSL-RNMAMAQSSHHLNLLQSVVGTS 66
           + +  ++ + A +  S   +   + D++ +IVYMG+L   +     SHH ++LQ V G S
Sbjct: 6   VSYCLLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGES 65

Query: 67  SETEGLYVRSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFR- 126
           S  E   VR+Y RSFNGFAA LT SERE LA+M+ VVSVFP+K LK QTT SW+++G + 
Sbjct: 66  S-IEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKE 125

Query: 127 -RKIKRDVAGETDVIIGSIDTGIWSESESFN----------------------------- 186
            ++ KR+   E+D IIG ID+GI+ ES+SF+                             
Sbjct: 126 SKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIG 185

Query: 187 ------------DDARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYK 246
                       + ARD +GHGSHTASTAA  AVKH SFYG+  G ARG VP+ARIA YK
Sbjct: 186 ARYYTPKLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYK 245

Query: 247 VCD---SGCHETDVLAAFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILT 306
           VCD    GC    +LAAFDDAIAD VD+ITISI G  ++PF+ D IAIG+FHAMA+GIL 
Sbjct: 246 VCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILI 305

Query: 307 VHSAGSDGPAEGSVGSVAPWLFLSTPLGIVLLGLQHSELLPSK---QDETSIDLRNKCF- 366
           V+SAG+ GP   +V S+APW+F           +    L   K   +   S DL  K + 
Sbjct: 306 VNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTVGRSVNSFDLNGKKYP 365

Query: 367 -------------KNLRFCSKSC---------SVLRPWPQLLD---AGDHEAGHIRPRRR 426
                         +  FCS  C          VL   PQ  D   A    A  +R  R 
Sbjct: 366 LVYGKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHRT 425

Query: 427 NSLRLFTCSFS-------------FRCSWRPKIC-------------------------- 486
           +   +F+   S                +  PK                            
Sbjct: 426 DVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTI 485

Query: 487 --------------------------------QVLYNV--WDFHVLPHVAGVAAYVKNFH 546
                                           +V Y+V        PHVAGVAAY+K+FH
Sbjct: 486 IPDILKPDITAPGSEIVAAYSPDAPPSISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 545

Query: 547 QNWSPSAIKSAIMTTAKQIISTDG--MHIGECLYGSGQINPKQAIKPGLVYETSEEDYVN 595
             WSPS I+SAIMTTA  + ++      + E  YG+G ++P  AI PGLVYE ++ D++ 
Sbjct: 546 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 605

[Close]

BLAST of Sgr023230 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 392.5 bits (1007), Expect = 6.1e-109
Identity = 285/727 (39.20%), Postives = 378/727 (51.99%), Query Frame = 0


Query: 15  LLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMA-MAQSSHHLNLLQSVVGTSSETEGLYV 74
           LL  + SS   SA I+ D +V+IVYMGSL + A    +S H+++LQ V G SS  EG  V
Sbjct: 14  LLVLLLSSV--SAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESS-IEGRLV 73

Query: 75  RSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFR--RKIKRDV 134
           RSY RSFNGFAA LT+SER  +A +EGVVSVFP+KIL+  TT SWD++G +  +  KR++
Sbjct: 74  RSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNL 133

Query: 135 AGETDVIIGSIDTGIWSESESFND-----------------------------------D 194
           A E+D IIG IDTGIW ES+SF+D                                    
Sbjct: 134 AIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEG 193

Query: 195 ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVC-DSGCHETDVLA 254
            RD+ GHG+HTASTAA  AVK  SF+GI  G  RG VP++RIAAYKVC DSGC    +L+
Sbjct: 194 TRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLS 253

Query: 255 AFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGPAEGSVGS 314
           +FDDAIADGVDLITISI     + F+ D IAIG+FHAMA+GILTV SAG+ GP   +V  
Sbjct: 254 SFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSH 313

Query: 315 VAPWLFL----STPLGIVLLGLQHSELLPSKQDETSIDLRNKCF---------------K 374
           VAPW+F     +T  G +   +  +    + +   + D++ K +               K
Sbjct: 314 VAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAK 373

Query: 375 NLRFCSKSC--------SVL----------------------RPWPQLLDAGDHEAGHIR 434
               C+ +C         +L                       P P +       A  ++
Sbjct: 374 TAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLK 433

Query: 435 PRRRNSLRLF------------------------TCSFSFRCS-------WRPKIC---- 494
            +   SL  +                          SFS R          +P I     
Sbjct: 434 AKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGV 493

Query: 495 ------------------QVLYNVWD--FHVLPHVAGVAAYVKNFHQNWSPSAIKSAIMT 554
                             +V Y+V+       PHVAGVAAYVK F+  WSPS I+SAIMT
Sbjct: 494 EILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMT 553

Query: 555 TAKQIISTDGMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYDSKAIELIS 598
           TAK      G+   E  YG+G ++P  A+ PGLVYE  + D++  L G  Y SK +++IS
Sbjct: 554 TAK----GRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIIS 613

[Close]

BLAST of Sgr023230 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 391.3 bits (1004), Expect = 1.4e-108
Identity = 284/728 (39.01%), Postives = 379/728 (52.06%), Query Frame = 0


Query: 15  LLAAVCSSTLCSAAINGDRKVHIVYMGSLRNMA-MAQSSHHLNLLQSVVGTSSETEGLYV 74
           LL  + SS   SA I+ D +V+IVYMGSL + A    +S H+++LQ V G SS  EG  V
Sbjct: 14  LLVLLLSSV--SAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESS-IEGRLV 73

Query: 75  RSYGRSFNGFAAELTDSEREKLAAMEGVVSVFPSKILKTQTTRSWDYVGFR--RKIKRDV 134
           RSY RSFNGFAA LT+SER  +A +EGVVSVFP+KIL+  TT SWD++G +  +  KR++
Sbjct: 74  RSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNL 133

Query: 135 AGETDVIIGSIDTGIWSESESFND-----------------------------------D 194
           A E+D IIG IDTGIW ES+SF+D                                    
Sbjct: 134 AIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEG 193

Query: 195 ARDSIGHGSHTASTAARRAVKHASFYGIAAGVARGAVPSARIAAYKVC-DSGCHETDVLA 254
            RD+ GHG+HTASTAA  AVK  SF+GI  G  RG VP++RIAAYKVC DSGC    +L+
Sbjct: 194 TRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLS 253

Query: 255 AFDDAIADGVDLITISISGVCATPFKYDGIAIGSFHAMARGILTVHSAGSDGPAEGSVGS 314
           +FDDAIADGVDLITISI     + F+ D IAIG+FHAMA+GILTV SAG+ GP   +V  
Sbjct: 254 SFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSH 313

Query: 315 VAPWLFL----STPLGIVLLGLQHSELLPSKQDETSIDLRNKCF---------------K 374
           VAPW+F     +T  G +   +  +    + +   + D++ K +               K
Sbjct: 314 VAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAK 373

Query: 375 NLRFCSKSC--------SVL----------------------RPWPQLLDAGDHEAGHIR 434
               C+ +C         +L                       P P +       A  ++
Sbjct: 374 TAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLK 433

Query: 435 PRRRNSLRLF------------------------TCSFSFRCS-------WRPKIC---- 494
            +   SL  +                          SFS R          +P I     
Sbjct: 434 AKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGV 493

Query: 495 ------------------QVLYNVWD--FHVLPHVAGVAAYVKNFHQNWSPSAIKSAIMT 554
                             +V Y+V+       PHVAGVAAYVK F+  WSPS I+SAIMT
Sbjct: 494 EILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMT 553

Query: 555 TAKQIISTD-GMHIGECLYGSGQINPKQAIKPGLVYETSEEDYVNLLRGNGYDSKAIELI 598
           TA  + +   G+   E  YG+G ++P  A+ PGLVYE  + D++  L G  Y SK +++I
Sbjct: 554 TAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKII 613

The following BLAST results are available for this feature:

BLAST of Sgr023230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022995031.1	4.1e-163	48.25	subtilisin-like protease SBT4.3 [Cucurbita maxima]	[more]
XP_022958362.1	1.2e-162	49.13	subtilisin-like protease SBT4.3 [Cucurbita moschata]	[more]
KAG6605933.1	2.0e-162	48.86	Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022995032.1	1.7e-161	48.85	subtilisin-like protease SBT4.3 [Cucurbita maxima]	[more]
XP_023533940.1	1.1e-160	48.32	subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sgr023230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FIF8	1.5e-112	40.34	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S...	[more]
Q9FIM6	1.0e-108	40.25	Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 S...	[more]
Q9FIG1	1.3e-108	39.30	Subtilisin-like protease SBT4.11 OS=Arabidopsis thaliana OX=3702 GN=SBT4.11 PE=2...	[more]
F4JA91	1.7e-108	38.43	Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S...	[more]
Q8L7D2	1.9e-107	39.01	Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2...	[more]

BLAST of Sgr023230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K0V8	2.0e-163	48.25	subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3...	[more]
A0A6J1H1M3	5.7e-163	49.13	subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE...	[more]
A0A6J1JXM0	8.2e-162	48.85	subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3...	[more]
A0A6J1H1W0	4.7e-157	47.69	subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE...	[more]
A0A5C7IY30	2.0e-128	42.07	Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_002770 PE=3 SV=1	[more]

BLAST of Sgr023230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59130.2	7.7e-112	40.28	Subtilase family protein	[more]
AT5G59190.1	2.9e-111	40.06	subtilase family protein	[more]
AT3G46840.1	1.2e-109	38.43	Subtilase family protein	[more]
AT5G59090.2	6.1e-109	39.20	subtilase 4.12	[more]
AT5G59090.1	1.4e-108	39.01	subtilase 4.12	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 149..275 e-value: 3.1E-10 score: 39.8
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 35..112 e-value: 1.0E-11 score: 45.4
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 384..453 e-value: 9.7E-24 score: 85.9 coord: 156..281 e-value: 5.9E-44 score: 152.6
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 76..498
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 488..594 e-value: 1.2E-21 score: 76.7
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 18..112 e-value: 1.3E-21 score: 78.9
None	No IPR available	GENE3D	2.60.40.2310		coord: 457..597 e-value: 2.1E-33 score: 116.8
None	No IPR available	PANTHER	PTHR10795:SF685	SUBTILISIN-LIKE PROTEASE SBT4.3	coord: 156..281
None	No IPR available	PANTHER	PTHR10795:SF685	SUBTILISIN-LIKE PROTEASE SBT4.3	coord: 35..156 coord: 384..597
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 117..440 score: 16.187164
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 156..281
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 35..156 coord: 384..597

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr023230.1	Sgr023230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity