Sgr023005 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr023005
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionArgininosuccinate lyase
Locationtig00000729: 1851667 .. 1855996 (-)
RNA-Seq ExpressionSgr023005
SyntenySgr023005
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAAACTCGATGCTCCGTCCGCTGCTGAGGCCGGCTCGTCGATCTCCAAGAAGCCCGAGGCTAAGGAAATCAAGCTCTGGGGTGGTCGCTTCCAGGAGAGCGTCACCGACGCCGTTGAACGCTTTACTGAGTCGATCTCATACGATAAGGACCTTTACAAGCATGACATCTGCGGCAGTCGTGCTCATGCATCCATGCTCGCCAAGCAGGTCTTTCCACCTCTCATTTTCATTTTTGTGTCTTCTGTTCAGCTCTACTCTGTTTGATTCCCGGCAAGGAATGCTTACAACTTATCAATAGTGTTTGTACTATCATAATGGGACCGAATCACGATGATGAGCTAAATATTTGCTCAAATTTGATCTATTATATCCTGAAAATTTCTCAGTTGCCATACGCATTCATGATAGTAGTTTGTGCTTTTGGTTGCAGGGATTGATGAGTATCAGTGACAGAGACAGTATTCTAGCTGGTCTCGATGAGATCGAGAGGCGTATTCGAAATGGCGAGTTCGTCTGGAGAGCTGATAGGGAAGATGTTCACATGAACATTGAAGCTGCTCTTACTGATTTGATTGGCGAACCTGCCAAGAAACTGCATACTGCTCGGAGCCGCAACGACCAGGTTGCGACTGATTTTCGCTTGTGGTGCCGCGATGCAATTGATTCGATCGTTGAGAGGATTAAAGATCTTCAGGTTGCAATGGTGGACTTGGCAATTAAGAACGCCGGAACTATTGTTCCAGGTTATACGCATTTGCAGAGAGCACAACCCGTCCTACTTCAGCATCTTCTCTTATCATTCGTTGAGCAGGTATTTAGCTACTATTCTAGGTCATCATCTTAAGTCATGGCTGAAATCCTGAAATAAATGGTTGCTTTTCTGCTGGTAGTGTTAGCTGCATGGTTTCTATTTCGCCTGAAACTTGAGAGGAGAGTCATGGGACCTAATAACTGCATCCTCGACGTAGGAGATTGTGTATGCGTGTGTGAAAGCTTGCTATACCTTAACCTTCTTGGAGGTGAAGCATCAGAAATTCTCCCCATACTTTCCCTGCAGAAGCTTTAAACATCTCACTTGAATCTACAATCCTCTTCATAAAACATTTCCCACATATTTTGACTTGATTTTTAAGACATTTCTTCTTCACATTCATAAACTCCCTTTCGAGACTGACGACGTGATGGTTACTTATAAAACTATACAAGATTCTATATTAGAATTTTAAAAGACCTATTGATTTATCTTTTTCCTTAGCTTAAGGAACAAATCCTAAGTTAGGATTAAAGATTATGCTAGTATTCACTTTTGCTAAAACCTACAGGAGACTGATTCTGGAAAATTTCATTACATGTTTTGTAGCTTGATCGGATGCTGGTCGTTTGCTGGATTGCAGAGTTAGACTGAATTTCTGCCCCTTGGGTGCTTGTGCATTGGCTGGTACTGGCCTCCCAATTGATCGATTCATGACTTCACGTGCTTTAGGATTCTCATCTCCTTTGAGAAACAGGTTTGTTGTCTAGTATTTTTATTTGTCAAGGTTGTTAAAAGTGGTGATTCTGATGGCTGGAATATCATGTTTTTCCCTCTGCAGGGTGTAATTTTGTAATTTCCCCCCTCCCGTGATTTCTGGGGGAATCTGTAATAGTTGTTAGGTCCCTTGCATATTATGTGACTTGAATATAAATGGTAGTAATAACATTACGGATTTCCAAATTCATATTTGTGAAAACAAGTTGCTGGAGGAGAATTCAACATAATTGAACTTCATTTACACACTTAGTTTTTCAAAACTTTTGATCACGTGGATTGGATGAACTAACGTAGGTTTTTAAGCATTTAGTTTGCACAAATGGGCTTTGGTGGCAGGGACTAGCTTATATTCTTTCGCATCTACTTGATCAAAACATTACTTTTTCATCTGTAATTCATGTCTACCTTTTCTGTAATTCCTTATATTTTTCAATCCGACTCTGTTGAATAACTTGTAAATTTTGTTGTCTCGAATAGTATTGATGCTGTTTCAGACCGGGATTTTGCAATGGAATTTCTTTCTGCCAATTCTATTACAGCCATCCATCTTTCTCGTCTGGGAGAAGAATGGGTATTGTGGTCCTCCGAGGAGTTTGGATTTATCACACCTAATGACGCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCGGATCCAATGGAACTTGTTCGTGGAAAGTCAGGAAGAGTCATAGGTGATTTAGTAACCCTTCTAACATTATGCAAAGGACTTCCTCTTGCATACAACCGTGATCTGCAGGTTTGAAATTTTTTACAGGTCAAATTTCCTTTTCAGAAAACTTCTGTCATCTTCTTTTTGATTGAATAAGTTGTTTGTGTTGAGCAGGAAGATAAGGAACCTGTATTTGATAGTGTTAAGACAGTTTTAGGGATGCTTGAAGTTTCAGCTGAGTTTGCACGGAATATTAGCTTCAATCAGGACAGAATAAAGAAGGCATTACCTGCCGGTCATCTTGATGCGACCACACTTGCGGACTATCTCGTGCATAAGGTTCGTAGCAGTATATTGCGTTTTATTTCTCCTTGTTAGAAGAGCTTAAGTATTAAAAGTATTTTAAATATTGAATTTGCTTATAAATTTTTACCTGGACGCCCTTGGAAGAATCTTGTTAGTTATGATAAAACTGGAATCGATTTTCATAACTCCTCCAGGTAAAAGAAAAGTCGTTGGCATAATGGTTCTTTGAACACAGATAGGGGTTTACATTTCCCTATATCAATTTCTTTGGTTATTTTCTCTCTGTTTTTATTTATTTATTTATTTTATTATTATTTTTTTCCCGGGGGGGGGGGGGGGGGGTGTTGGCGGGGTTACAAAACTATAGAGAAATGGTTTAACTCAACATGTAACATTTATAGCAAGATCACAATCAGATATGTAGATCATGAGCATGGGTGTTTGAGAAAGAGGTGTAGGTTTGAAAATTGCAGAATGCTTTTGAAACATAAAATTTAAAACACAATTGATAATTGAGAACTCTGAAGAATTGACAGCATAGTTTTTTAACTTAAAAAATTTTAAACAAGAGATTGAAAGTTGAAAATTCCGAAAAATTGCACCCAGAACATAAACGACCAAAGATATTAAAGGCGACTTAAATAAGGGTAAGAAATCTTAACATTTTTCAACCTTAAGAAACAGCTGCTATAAGTTAGTTTTAATGCAATAATCCAGATCTCTCTAGTCTCTCCCCCTCCCTATATTTGATCAATAAATATTTATCTATATAATCTGGAGGAGAAAAAAATTATGATGAAACATAATGTCCGTGGAACCACCATGGGTTGGTCTAGTGATTATTGGAACCAGTATAAAAGTAAAGGGACTTAAAAGAATGGGTTCAAACCATGGCGGCTACCTACCTAAGATTTAAAATCCTATTGGTTTTCTTGACGACTAAATGTTGTAGGGTTCATATTTGTCTTGTGAGATTAGTTAAAGTACACACAAGCTGACTCAGACACTCACGAATATCAAAATAATAATGATGATGATGATACCTAGGGGTCTTTTATGATTTGGTTCATAGCATAGCATGCATTTCCCACAAAGTTTGTACATTGCTTAAGAATCTTTCAAGGACCGCAATGTTGCAACTTGCGGTATGCTTTTATAAAATAGGGGCTTTTAGATGAGCATCGGGTTGATTACCTACGACCATGTGCTGACCCAACGGCCACATGGTCGGCTCTACAATTCTCAAGGGTTTATTTGGCTTATTTGGCAGTTGTTTCCAAACTGTTTGTTAGAACAACTTTAGGTAATTTATAAAAGCAAACACTATTATGTGCTCAAAATCATTATTCGTAATTTTATAATTTGACTAAAAAACAATTTCAATTATTTTAAAATATAGGTCATCCATGAAATGAACTATAAGAAAACAAGATAAAAACTTAAAATAAAAAAAACAGTTATTCACACACGCTTTAACTGAATTTTTATTCTAAAAGCTTGCAGTTATTCAAACAGCTTCTAACTCTCTGATTTCAATTCCCCTTCTTTAACAGGGTATACCTTTCAGAACATCTCATGATATTGTAGGCAAATCTGTTGCCATGTGTCTTGGAAAGAACTGCCAGCTGAAGGGCCTAAGTCTCGATGATCTGCGAACGTTGAGTCCAATCATTGAGAAGGATGTTTACGAATTTCTTGGAGTAGAAAATGCAATAAGCAAATTTTCCTCATATGGCTCCACAGGATCAGAATGTGTTGCCAGTCAGCTTGAGTATTGGATCGCAAAACTCGGAATTAGTCAGTAG

mRNA sequence

ATGTCTAAACTCGATGCTCCGTCCGCTGCTGAGGCCGGCTCGTCGATCTCCAAGAAGCCCGAGGCTAAGGAAATCAAGCTCTGGGGTGGTCGCTTCCAGGAGAGCGTCACCGACGCCGTTGAACGCTTTACTGAGTCGATCTCATACGATAAGGACCTTTACAAGCATGACATCTGCGGCAGTCGTGCTCATGCATCCATGCTCGCCAAGCAGGGATTGATGAGTATCAGTGACAGAGACAGTATTCTAGCTGGTCTCGATGAGATCGAGAGGCGTATTCGAAATGGCGAGTTCGTCTGGAGAGCTGATAGGGAAGATGTTCACATGAACATTGAAGCTGCTCTTACTGATTTGATTGGCGAACCTGCCAAGAAACTGCATACTGCTCGGAGCCGCAACGACCAGGTTGCGACTGATTTTCGCTTGTGGTGCCGCGATGCAATTGATTCGATCGTTGAGAGGATTAAAGATCTTCAGGTTGCAATGGTGGACTTGGCAATTAAGAACGCCGGAACTATTGTTCCAGGTTATACGCATTTGCAGAGAGCACAACCCGTCCTACTTCAGCATCTTCTCTTATCATTCGTTGAGCAGGTATTTAGCTACTATTCTAGAGTTAGACTGAATTTCTGCCCCTTGGGTGCTTGTGCATTGGCTGGTACTGGCCTCCCAATTGATCGATTCATGACTTCACGTGCTTTAGGATTCTCATCTCCTTTGAGAAACAGTATTGATGCTGTTTCAGACCGGGATTTTGCAATGGAATTTCTTTCTGCCAATTCTATTACAGCCATCCATCTTTCTCGTCTGGGAGAAGAATGGGTATTGTGGTCCTCCGAGGAGTTTGGATTTATCACACCTAATGACGCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCGGATCCAATGGAACTTGTTCGTGGAAAGTCAGGAAGAGTCATAGGTGATTTAGTAACCCTTCTAACATTATGCAAAGGACTTCCTCTTGCATACAACCGTGATCTGCAGGAAGATAAGGAACCTGTATTTGATAGTGTTAAGACAGTTTTAGGGATGCTTGAAGTTTCAGCTGAGTTTGCACGGAATATTAGCTTCAATCAGGACAGAATAAAGAAGGCATTACCTGCCGGTCATCTTGATGCGACCACACTTGCGGACTATCTCGTGCATAAGGGTATACCTTTCAGAACATCTCATGATATTGTAGGCAAATCTGTTGCCATGTGTCTTGGAAAGAACTGCCAGCTGAAGGGCCTAAGTCTCGATGATCTGCGAACGTTGAGTCCAATCATTGAGAAGGATGTTTACGAATTTCTTGGAGTAGAAAATGCAATAAGCAAATTTTCCTCATATGGCTCCACAGGATCAGAATGTGTTGCCAGTCAGCTTGAGTATTGGATCGCAAAACTCGGAATTAGTCAGTAG

Coding sequence (CDS)

ATGTCTAAACTCGATGCTCCGTCCGCTGCTGAGGCCGGCTCGTCGATCTCCAAGAAGCCCGAGGCTAAGGAAATCAAGCTCTGGGGTGGTCGCTTCCAGGAGAGCGTCACCGACGCCGTTGAACGCTTTACTGAGTCGATCTCATACGATAAGGACCTTTACAAGCATGACATCTGCGGCAGTCGTGCTCATGCATCCATGCTCGCCAAGCAGGGATTGATGAGTATCAGTGACAGAGACAGTATTCTAGCTGGTCTCGATGAGATCGAGAGGCGTATTCGAAATGGCGAGTTCGTCTGGAGAGCTGATAGGGAAGATGTTCACATGAACATTGAAGCTGCTCTTACTGATTTGATTGGCGAACCTGCCAAGAAACTGCATACTGCTCGGAGCCGCAACGACCAGGTTGCGACTGATTTTCGCTTGTGGTGCCGCGATGCAATTGATTCGATCGTTGAGAGGATTAAAGATCTTCAGGTTGCAATGGTGGACTTGGCAATTAAGAACGCCGGAACTATTGTTCCAGGTTATACGCATTTGCAGAGAGCACAACCCGTCCTACTTCAGCATCTTCTCTTATCATTCGTTGAGCAGGTATTTAGCTACTATTCTAGAGTTAGACTGAATTTCTGCCCCTTGGGTGCTTGTGCATTGGCTGGTACTGGCCTCCCAATTGATCGATTCATGACTTCACGTGCTTTAGGATTCTCATCTCCTTTGAGAAACAGTATTGATGCTGTTTCAGACCGGGATTTTGCAATGGAATTTCTTTCTGCCAATTCTATTACAGCCATCCATCTTTCTCGTCTGGGAGAAGAATGGGTATTGTGGTCCTCCGAGGAGTTTGGATTTATCACACCTAATGACGCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCGGATCCAATGGAACTTGTTCGTGGAAAGTCAGGAAGAGTCATAGGTGATTTAGTAACCCTTCTAACATTATGCAAAGGACTTCCTCTTGCATACAACCGTGATCTGCAGGAAGATAAGGAACCTGTATTTGATAGTGTTAAGACAGTTTTAGGGATGCTTGAAGTTTCAGCTGAGTTTGCACGGAATATTAGCTTCAATCAGGACAGAATAAAGAAGGCATTACCTGCCGGTCATCTTGATGCGACCACACTTGCGGACTATCTCGTGCATAAGGGTATACCTTTCAGAACATCTCATGATATTGTAGGCAAATCTGTTGCCATGTGTCTTGGAAAGAACTGCCAGCTGAAGGGCCTAAGTCTCGATGATCTGCGAACGTTGAGTCCAATCATTGAGAAGGATGTTTACGAATTTCTTGGAGTAGAAAATGCAATAAGCAAATTTTCCTCATATGGCTCCACAGGATCAGAATGTGTTGCCAGTCAGCTTGAGTATTGGATCGCAAAACTCGGAATTAGTCAGTAG

Protein sequence

MSKLDAPSAAEAGSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGYTHLQRAQPVLLQHLLLSFVEQVFSYYSRVRLNFCPLGACALAGTGLPIDRFMTSRALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIAKLGISQ
Homology
BLAST of Sgr023005 vs. NCBI nr
Match: XP_022131833.1 (argininosuccinate lyase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 874.4 bits (2258), Expect = 4.5e-250
Identity = 444/490 (90.61%), Postives = 458/490 (93.47%), Query Frame = 0

Query: 1   MSKLDAPSAAE-------AGSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           M++LD  SA E       AGSS+SKK E KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           YKHDI GSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA
Sbjct: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTI 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI ERIKDLQVA+++LAIKNAG I
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180

Query: 181 VPGYTHLQRAQPVLLQHLLLSFVEQVFS-----YYSRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQHLLLSFVEQ+          RVRLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSRALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPN 300
           MTS ALGFSSPLRNSIDAVSDRDFAMEFLSANSITA+HLSRLGEEWVLWSSEEFGFITPN
Sbjct: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK V GMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLE 479
           SVAMCLG+NCQLK LSLDDLRTLSPI + DVYEFLGVENA++KFSSYGSTGSECVASQLE
Sbjct: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480

BLAST of Sgr023005 vs. NCBI nr
Match: XP_038886091.1 (argininosuccinate lyase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 869.0 bits (2244), Expect = 1.9e-248
Identity = 441/488 (90.37%), Postives = 456/488 (93.44%), Query Frame = 0

Query: 1   MSKLDAPSAAE-------AGSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           M+KLD PSA++       AGS  +KKP AKEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTKLDVPSASQPTGATSAAGSCATKKPGAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           YKHDI GSRAHASMLAKQGLMSISDRD+ILAGLDEIERRIRNGEFVWRADREDVHMNIEA
Sbjct: 61  YKHDISGSRAHASMLAKQGLMSISDRDNILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTI 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKD QVAMVDLAIKNAG I
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAIKNAGII 180

Query: 181 VPGYTHLQRAQPVLLQHLLLSFVEQVFSYYSRV-----RLNFCPLGACALAGTGLPIDRF 240
           VPGYTHLQRAQPVLLQHLLL+FVEQ+     R+     RLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSRALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPN 300
           MTS AL FSSPLRNSIDAVSDRDFAMEFLSANSI AIHLSRLGEEWVLWSSEEFGFITPN
Sbjct: 241 MTSSALEFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPN 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKS RVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLE 477
           SVAMCLGKNCQLK LSLD+LRTLSPI + DVYEFLGVEN+++KFSSYGSTGSECVA+QL+
Sbjct: 421 SVAMCLGKNCQLKDLSLDELRTLSPIFDDDVYEFLGVENSVNKFSSYGSTGSECVANQLQ 480

BLAST of Sgr023005 vs. NCBI nr
Match: KAA0065095.1 (argininosuccinate lyase [Cucumis melo var. makuwa])

HSP 1 Score: 858.6 bits (2217), Expect = 2.6e-245
Identity = 432/484 (89.26%), Postives = 454/484 (93.80%), Query Frame = 0

Query: 1   MSKLDAPSAAE---AGSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           M+KLD  SA++   A S+ SK+PEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGASSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  ICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSISDRDSILAGLDEIERRI+NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIQNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKD QVAMVDLA+KNAG IVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHLLLSFVEQVFSYYSRV-----RLNFCPLGACALAGTGLPIDRFMTSR 240
           THLQRAQPVLLQHLLL+FVEQ+     R+     RLNFCPLGACALAGTGLPIDRFMTS 
Sbjct: 181 THLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           AL FSSPLRNSIDAVSDRDFA+EFLSANS+ AIHLSRLGEEWVLW SEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSVMAIHLSRLGEEWVLWCSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKS RVIGD+VTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVS EFARNISFNQDRIKKALPAGHLDATTLADYLVHKG+PFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSTEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGMPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIA 477
           CLGKNCQLK LS+D+LRTLSPI + DVYEFLGVENA++KFSSYGSTGSECVASQL+YW+ 
Sbjct: 421 CLGKNCQLKDLSIDELRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWME 480

BLAST of Sgr023005 vs. NCBI nr
Match: XP_023546913.1 (argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857.8 bits (2215), Expect = 4.4e-245
Identity = 436/486 (89.71%), Postives = 452/486 (93.00%), Query Frame = 0

Query: 1   MSKLDAPSAAEA---GSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           M+  D  SAAEA    S+ +KKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL+KHD
Sbjct: 1   MTIFDVSSAAEATGETSAAAKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLFKHD 60

Query: 61  ICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSISDRD ILAGLDEIE+RIRNGEFVWRADREDVHMNIEAALTD
Sbjct: 61  ISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRIRNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI+ERIKD QVAMVDLAIKN G IVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIIERIKDFQVAMVDLAIKNKGIIVPGY 180

Query: 181 THLQRAQPVLLQHLLLSFVEQVFSYYSRV-----RLNFCPLGACALAGTGLPIDRFMTSR 240
           THLQRAQPVLLQHLLL+FVEQ+     R+     RLNFCPLGACALAGTGLPIDRFMTS 
Sbjct: 181 THLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           ALGFSSPLRNSIDAVSDRDFAMEFLSANSI AIHLSRLGEEWVLWSSEEFGFI P+DAVS
Sbjct: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPSDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKS RVIGDLVTLL+LCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           V GMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM
Sbjct: 361 VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIA 479
           CLGKNCQLK LSL +LR LSPI ++DVYEFLGVENA++KFSSYGSTGSECVASQLEYWI 
Sbjct: 421 CLGKNCQLKDLSLAELRALSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLEYWIE 480

BLAST of Sgr023005 vs. NCBI nr
Match: XP_004152599.1 (argininosuccinate lyase, chloroplastic [Cucumis sativus] >KGN62752.1 hypothetical protein Csa_022166 [Cucumis sativus])

HSP 1 Score: 857.1 bits (2213), Expect = 7.4e-245
Identity = 433/484 (89.46%), Postives = 455/484 (94.01%), Query Frame = 0

Query: 1   MSKLDAPSAAE---AGSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           M+KLD  SA++   A S+ SK+PEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  ICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSI DRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIV RIKD QVAMVDLA+KNAG IVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHLLLSFVEQVFSYYSRV-----RLNFCPLGACALAGTGLPIDRFMTSR 240
           THLQRAQPVLLQH+LL+FVEQ+     R+     RLNFCPLGACALAGTGLPI+RFMTS 
Sbjct: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240

Query: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           AL FSSPLRNSIDAVSDRDFA+EFLSANSI AIHLSRLGEEWVLWSSEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKS RVIGD+VTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIA 477
           CLGKNCQLK LSL++LR+LSPI ++DVYEFLGVENA++KFSSYGSTGSECVASQL+YWI 
Sbjct: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480

BLAST of Sgr023005 vs. ExPASy Swiss-Prot
Match: Q9LEU8 (Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10920 PE=2 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 8.4e-207
Identity = 345/461 (74.84%), Postives = 407/461 (88.29%), Query Frame = 0

Query: 22  AKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDICGSRAHASMLAKQGLMSISDRDS 81
           +KE+KLWGGRF+ESVT+ VE+FTESIS+DK LYK DI GS+AHASMLA QGL++ SD+DS
Sbjct: 55  SKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSDKDS 114

Query: 82  ILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 141
           IL GLD+IER+I   +F WR DREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR
Sbjct: 115 ILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 174

Query: 142 LWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGYTHLQRAQPVLLQHLLLSFVEQVFS 201
           LWCRDAID+I+ +I++LQ A+V+LA+KN   IVPGYTHLQRAQPVLL H+LL+FVEQ+  
Sbjct: 175 LWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQLER 234

Query: 202 -----YYSRVRLNFCPLGACALAGTGLPIDRFMTSRALGFSSPLRNSIDAVSDRDFAMEF 261
                   R RLNF PLGACALAGTGLPIDRFMT+ ALGF+ P+RNSIDAVSDRDF +EF
Sbjct: 235 DAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFVLEF 294

Query: 262 LSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGR 321
           L  N+ T IHLSRLGEEWVLW+SEEFGF+TP+D+VSTGSSIMPQKKNPDPMELVRGKS R
Sbjct: 295 LYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGKSAR 354

Query: 322 VIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKK 381
           VIGDLVT+LTLCKGLPLAYNRD QEDKEP+FDS KT++GM++VSAEFA+N++FN+DRIKK
Sbjct: 355 VIGDLVTVLTLCKGLPLAYNRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDRIKK 414

Query: 382 ALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKGLSLDDLRTLSPIIE 441
           +LPAGHLDATTLADYLV KG+PFR+SHDIVGK V +C+ K C+L+ LSL++++ LSP+ E
Sbjct: 415 SLPAGHLDATTLADYLVKKGMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSPVFE 474

Query: 442 KDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIAKLGIS 478
           +DV+ FLGVEN+++KFSSYGSTGS CVA QL YW+ KL I+
Sbjct: 475 EDVFGFLGVENSVNKFSSYGSTGSNCVAEQLGYWVNKLNIT 515

BLAST of Sgr023005 vs. ExPASy Swiss-Prot
Match: Q6AR60 (Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) OX=177439 GN=argH PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.2e-125
Identity = 224/444 (50.45%), Postives = 300/444 (67.57%), Query Frame = 0

Query: 21  EAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDICGSRAHASMLAKQGLMSISDRD 80
           E K  KLWGGRF E++  +VE+F+ESISYD  LYK+DI GS+AHA+ML+ QG++S  + +
Sbjct: 3   EQKSKKLWGGRFSEAMAASVEKFSESISYDVRLYKYDIAGSKAHATMLSSQGIISPEELE 62

Query: 81  SILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDF 140
            I+AGL  IE  I  G F ++ + EDVHMNIE AL D IG    +LH ARSRNDQ+A DF
Sbjct: 63  QIIAGLSSIEADIEAGVFEFKTEYEDVHMNIEQALVDRIGAAGSRLHAARSRNDQIALDF 122

Query: 141 RLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGYTHLQRAQPVLLQHLLLSFVEQVF 200
           +++ RD  D +VE +     A   +  K  G I+PGYTH QRAQPVL+ H +L++ E   
Sbjct: 123 KMYLRDQCDHLVELLDGACRAFTVVGRKYLGDIMPGYTHTQRAQPVLITHHMLAYYEMFR 182

Query: 201 SYYSRV-----RLNFCPLGACALAGTGLPIDRFMTSRALGFSSPLRNSIDAVSDRDFAME 260
               R+     RLN  PLG  A+AGTGLPI+R   ++ALGF+    NS+D  +DRD+A+E
Sbjct: 183 RDRDRILDCRKRLNLSPLGCAAMAGTGLPINREQVAKALGFAGVTANSMDTSADRDYAIE 242

Query: 261 FLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSG 320
             S  ++  +HLSRL EE V WS+ E+ F+  +D+  TGSSIMPQKKNPD  EL+RGKSG
Sbjct: 243 LTSCLTMIQLHLSRLAEELVTWSTSEYKFVDISDSFCTGSSIMPQKKNPDIAELIRGKSG 302

Query: 321 RVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIK 380
           RV+G L++L+T+ KGLPL YNRD QEDKEPVFD++ TV   L ++AE   ++ FN  R  
Sbjct: 303 RVVGSLISLITMMKGLPLTYNRDQQEDKEPVFDAIDTVSASLSITAEMMAHMKFNTARCA 362

Query: 381 KALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKGLSLDDLRTLSPII 440
           +A   G + AT LADYLV K +PFR +H IVG +VA C+ K C+L  L+L +++  SP+I
Sbjct: 363 EATETGFITATDLADYLVMKDVPFRQAHHIVGSAVAACIAKGCELPDLTLTEMQEFSPVI 422

Query: 441 EKDVYEFLGVENAISKFSSYGSTG 460
           E DV+  L  E +++   S G TG
Sbjct: 423 ESDVFAVLTAEGSVNSRVSTGGTG 446

BLAST of Sgr023005 vs. ExPASy Swiss-Prot
Match: Q2LT96 (Argininosuccinate lyase OS=Syntrophus aciditrophicus (strain SB) OX=56780 GN=argH PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 5.2e-124
Identity = 232/454 (51.10%), Postives = 306/454 (67.40%), Query Frame = 0

Query: 19  KPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDICGSRAHASMLAKQGLMSISD 78
           K   ++ K WGGRFQE   + VE FT SIS+D+ LY++DI GS AHA ML +Q +++  +
Sbjct: 2   KDSTEKSKPWGGRFQEPTNELVEAFTASISFDRRLYRYDIEGSIAHARMLVRQEIINRKE 61

Query: 79  RDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVAT 138
             +I+ GL EI+R I  G F +    ED+HM IE  L   IG+   KLHT RSRNDQVA 
Sbjct: 62  GFAIVEGLKEIQRDIETGSFEFSPGDEDIHMAIEKDLIRRIGDAGAKLHTGRSRNDQVAL 121

Query: 139 DFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGYTHLQRAQPVLLQHLLLSFVEQ 198
           D RL+ R  I  I+E +K L+ A ++LA K A TI+PGYTHLQ+AQPVLL H LL++ E 
Sbjct: 122 DVRLYLRAEIKEILEFMKVLKGAFLELAKKEAETILPGYTHLQKAQPVLLAHYLLAWREM 181

Query: 199 VFSYYSRV-----RLNFCPLGACALAGTGLPIDRFMTSRALGFSSPLRNSIDAVSDRDFA 258
           +    SR+     R+N  PLGA ALAGT LPIDR   +R L F    RNS+D VSDRDF 
Sbjct: 182 LDRDESRLRDCYRRVNVLPLGAAALAGTSLPIDRAYVARLLKFPEISRNSMDTVSDRDFV 241

Query: 259 MEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGK 318
            EFL A+S+  +HLSR  E+ +LWSS EF F+  +DA +TGSSIMPQKKNPD  EL+RGK
Sbjct: 242 AEFLFASSLIMMHLSRFCEDLILWSSGEFNFVEISDAFTTGSSIMPQKKNPDVAELIRGK 301

Query: 319 SGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDR 378
           +GRV G+L+ LLTL KGLP+ YNRDLQEDKEP+FD+V TV   L++ AE  RN+ FN+++
Sbjct: 302 TGRVYGNLIALLTLLKGLPMTYNRDLQEDKEPLFDTVDTVKACLQILAEMIRNMKFNREK 361

Query: 379 IKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKGLSLDDLRTLSP 438
           +++    G   AT LA+YLV KGIPFR +H IVGK V+ C+    +L  LS+++ +   P
Sbjct: 362 MRQEAEGGFSTATDLAEYLVMKGIPFREAHGIVGKLVSFCIECKKELAQLSMEEFQRFCP 421

Query: 439 IIEKDVYEFLGVENAISKFSSYGSTGSECVASQL 468
           +I +D+++ L V N++    SYG T    V  Q+
Sbjct: 422 VINEDIHDRLSVSNSVRSRKSYGGTACRQVMEQI 455

BLAST of Sgr023005 vs. ExPASy Swiss-Prot
Match: Q3JDS2 (Argininosuccinate lyase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107) OX=323261 GN=argH PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 6.8e-124
Identity = 234/454 (51.54%), Postives = 303/454 (66.74%), Query Frame = 0

Query: 26  KLWGGRFQESVTDAVERFTESISYDKDLYKHDICGSRAHASMLAKQGLMSISDRDSILAG 85
           K W GRF E     VE FT SI +D  LY+ DI GS AHA MLAK G++S  + D+I+ G
Sbjct: 10  KPWSGRFHEPTDTFVEEFTASIGFDHRLYRQDIHGSIAHARMLAKVGVLSREECDAIIQG 69

Query: 86  LDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCR 145
           L+ I + I  G+F W    EDVHMNIEAALT+ IG   KKLHT RSRNDQ+ATD RL+ R
Sbjct: 70  LETIAQDISRGQFSWSVALEDVHMNIEAALTERIGPVGKKLHTGRSRNDQIATDIRLYLR 129

Query: 146 DAIDSIVERIKDLQVAMVDLAIKNAGTIVPGYTHLQRAQPVLLQHLLLSFVEQVFSYYSR 205
           DAID I  +++ LQ  ++ +A + A TI+PG+THLQ AQPV   H L+++ E +     R
Sbjct: 130 DAIDLITAQLQRLQEGLLAIAEREAATIMPGFTHLQTAQPVTFGHHLMAWFEMLLRDTGR 189

Query: 206 V-----RLNFCPLGACALAGTGLPIDRFMTSRALGFSSPLRNSIDAVSDRDFAMEFLSAN 265
           +     R+N  PLGA ALAGT  PIDR  T+  LGF     NS+DAVSDRDFA+EF +A 
Sbjct: 190 LADCRHRVNVLPLGAAALAGTTFPIDRAYTAELLGFDGIAENSLDAVSDRDFAIEFTAAG 249

Query: 266 SITAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGRVIGD 325
           ++   HLSR  EE VLWSS +F FIT  D   TGSSIMPQKKNPD  ELVRGK+GRV G 
Sbjct: 250 ALLMTHLSRFAEELVLWSSAQFDFITLPDRFCTGSSIMPQKKNPDVPELVRGKTGRVNGH 309

Query: 326 LVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKKALPA 385
           LV+LLTL KG PLAYN+D QEDKEP+FD+V T+LG L   A+    I+ N D++++A   
Sbjct: 310 LVSLLTLMKGQPLAYNKDNQEDKEPLFDTVDTLLGCLRAFADMIPAITTNPDKMREAATK 369

Query: 386 GHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKGLSLDDLRTLSPIIEKDVY 445
           G+  AT LADYLV KG+ FR +H+IVGK+VA+ + ++  L  L L  L+  SP+IE+DV+
Sbjct: 370 GYATATDLADYLVRKGVSFRDAHEIVGKAVALAIEQSKDLAALKLATLQDFSPVIEEDVF 429

Query: 446 EFLGVENAISKFSSYGSTGSECVASQLEYWIAKL 475
           + L +E +++  +  G T    V + ++    KL
Sbjct: 430 DVLTLEGSVAARNHLGGTAPAQVRAAIQRGREKL 463

BLAST of Sgr023005 vs. ExPASy Swiss-Prot
Match: Q2SQ67 (Argininosuccinate lyase OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=argH PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.6e-123
Identity = 232/443 (52.37%), Postives = 293/443 (66.14%), Query Frame = 0

Query: 26  KLWGGRFQESVTDAVERFTESISYDKDLYKHDICGSRAHASMLAKQGLMSISDRDSILAG 85
           KLWGGRF E+    VERFT S+ +D+ +Y HDI GS AHA+MLAK G+++  +RD I+ G
Sbjct: 9   KLWGGRFTEATDAFVERFTASVDFDRRMYHHDIRGSIAHATMLAKVGVLTEEERDQIING 68

Query: 86  LDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCR 145
           L EIE  I  G F W    EDVHMNIEA LT  IG   KKLHT RSRNDQVATD RL+ R
Sbjct: 69  LREIEAEIEAGAFEWSVKLEDVHMNIEARLTQKIGITGKKLHTGRSRNDQVATDIRLYLR 128

Query: 146 DAIDSIVERIKDLQVAMVDLAIKNAGTIVPGYTHLQRAQPVLLQHLLLSFVEQVFSYYSR 205
           D +D I   +  L  A+ DLA + A  I+PG+THLQ AQPV   H +L++ E +   +SR
Sbjct: 129 DEVDVIAAELLRLIAALADLAEREADAIMPGFTHLQTAQPVTFGHHMLAWAEMLKRDFSR 188

Query: 206 V-----RLNFCPLGACALAGTGLPIDRFMTSRALGFSSPLRNSIDAVSDRDFAMEFLSAN 265
           +     R N  PLGA ALAGT   IDR  TS  LGF+ P  NS+DAVSDRDFA+EF S  
Sbjct: 189 LADCRERFNVSPLGAAALAGTTYSIDRQYTSELLGFNGPAENSLDAVSDRDFAIEFCSFA 248

Query: 266 SITAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGRVIGD 325
           S+  +HLSR  EE VLW+S +F FI   D   TGSSIMPQKKNPD  EL+RGKSGRV G 
Sbjct: 249 SLLMMHLSRFSEELVLWTSAQFNFINLPDRFCTGSSIMPQKKNPDVPELIRGKSGRVSGH 308

Query: 326 LVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKKALPA 385
           L++LL L K  PLAYN+D QEDKEP+FD++ TV G L+   +    +S N+D + ++   
Sbjct: 309 LISLLMLMKSQPLAYNKDNQEDKEPLFDAIDTVKGCLKAYGDMMPAVSVNRDAMAESARR 368

Query: 386 GHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKGLSLDDLRTLSPIIEKDVY 445
           G   AT LADYLV KG+PFR +H++VGK+VA+ +     L  +SL++LR  S  IE DV+
Sbjct: 369 GFSTATDLADYLVRKGLPFRDAHEVVGKAVALGVSSGRDLSEMSLEELREFSADIEVDVF 428

Query: 446 EFLGVENAISKFSSYGSTGSECV 464
           E L +E +++  +  G T  E V
Sbjct: 429 EVLTLEGSVNARNHIGGTAPEQV 451

BLAST of Sgr023005 vs. ExPASy TrEMBL
Match: A0A6J1BUL3 (argininosuccinate lyase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111004891 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.2e-250
Identity = 444/490 (90.61%), Postives = 458/490 (93.47%), Query Frame = 0

Query: 1   MSKLDAPSAAE-------AGSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           M++LD  SA E       AGSS+SKK E KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           YKHDI GSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA
Sbjct: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTI 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI ERIKDLQVA+++LAIKNAG I
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180

Query: 181 VPGYTHLQRAQPVLLQHLLLSFVEQVFS-----YYSRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQHLLLSFVEQ+          RVRLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSRALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPN 300
           MTS ALGFSSPLRNSIDAVSDRDFAMEFLSANSITA+HLSRLGEEWVLWSSEEFGFITPN
Sbjct: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK V GMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLE 479
           SVAMCLG+NCQLK LSLDDLRTLSPI + DVYEFLGVENA++KFSSYGSTGSECVASQLE
Sbjct: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480

BLAST of Sgr023005 vs. ExPASy TrEMBL
Match: A0A5A7VGX5 (Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004280 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 432/484 (89.26%), Postives = 454/484 (93.80%), Query Frame = 0

Query: 1   MSKLDAPSAAE---AGSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           M+KLD  SA++   A S+ SK+PEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGASSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  ICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSISDRDSILAGLDEIERRI+NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIQNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKD QVAMVDLA+KNAG IVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHLLLSFVEQVFSYYSRV-----RLNFCPLGACALAGTGLPIDRFMTSR 240
           THLQRAQPVLLQHLLL+FVEQ+     R+     RLNFCPLGACALAGTGLPIDRFMTS 
Sbjct: 181 THLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           AL FSSPLRNSIDAVSDRDFA+EFLSANS+ AIHLSRLGEEWVLW SEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSVMAIHLSRLGEEWVLWCSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKS RVIGD+VTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVS EFARNISFNQDRIKKALPAGHLDATTLADYLVHKG+PFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSTEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGMPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIA 477
           CLGKNCQLK LS+D+LRTLSPI + DVYEFLGVENA++KFSSYGSTGSECVASQL+YW+ 
Sbjct: 421 CLGKNCQLKDLSIDELRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWME 480

BLAST of Sgr023005 vs. ExPASy TrEMBL
Match: A0A0A0LPM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370510 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 3.6e-245
Identity = 433/484 (89.46%), Postives = 455/484 (94.01%), Query Frame = 0

Query: 1   MSKLDAPSAAE---AGSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           M+KLD  SA++   A S+ SK+PEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  ICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSI DRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIV RIKD QVAMVDLA+KNAG IVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHLLLSFVEQVFSYYSRV-----RLNFCPLGACALAGTGLPIDRFMTSR 240
           THLQRAQPVLLQH+LL+FVEQ+     R+     RLNFCPLGACALAGTGLPI+RFMTS 
Sbjct: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240

Query: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           AL FSSPLRNSIDAVSDRDFA+EFLSANSI AIHLSRLGEEWVLWSSEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKS RVIGD+VTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIA 477
           CLGKNCQLK LSL++LR+LSPI ++DVYEFLGVENA++KFSSYGSTGSECVASQL+YWI 
Sbjct: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480

BLAST of Sgr023005 vs. ExPASy TrEMBL
Match: A0A1S3BBH8 (argininosuccinate lyase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103488122 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 3.6e-245
Identity = 431/484 (89.05%), Postives = 453/484 (93.60%), Query Frame = 0

Query: 1   MSKLDAPSAAE---AGSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           M+KLD  SA++   A S+ SK+PEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGASSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  ICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSISDRDSILAGLDEIERRI+NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIQNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKD QVAMVDLA+KNAG IVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHLLLSFVEQVFSYYSRV-----RLNFCPLGACALAGTGLPIDRFMTSR 240
           THLQRAQPVLLQHLL +FVEQ+     R+     RLNFCPLGACALAGTGLPIDRFMTS 
Sbjct: 181 THLQRAQPVLLQHLLFAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           AL FSSPLRNSIDAVSDRDFA+EFLSANS+ AIHLSRLGEEWVLW SEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSVMAIHLSRLGEEWVLWCSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKS RVIGD+VTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVS EFARNISFNQDRIKKALPAGHLDATTLADYLVHKG+PFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSTEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGMPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIA 477
           CLGKNCQLK LS+D+LRTLSPI + DVYEFLGVENA++KFSSYGSTGSECVASQL+YW+ 
Sbjct: 421 CLGKNCQLKDLSIDELRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWME 480

BLAST of Sgr023005 vs. ExPASy TrEMBL
Match: A0A6J1HD89 (argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462454 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.8e-244
Identity = 434/486 (89.30%), Postives = 451/486 (92.80%), Query Frame = 0

Query: 1   MSKLDAPSAAEA---GSSISKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           M+  D  SAAEA    S+ +KKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL+KHD
Sbjct: 1   MTIFDVSSAAEATGETSAAAKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLFKHD 60

Query: 61  ICGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSISDRD ILAGLDEIE+RI NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  ISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRILNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI+ERIKD QVAMVDLAIKN G IVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIIERIKDFQVAMVDLAIKNKGIIVPGY 180

Query: 181 THLQRAQPVLLQHLLLSFVEQVFSYYSRV-----RLNFCPLGACALAGTGLPIDRFMTSR 240
           THLQRAQPVLLQHLLL+F+EQ+     R+     RLNFCPLGACALAGTGLPIDRFMTS 
Sbjct: 181 THLQRAQPVLLQHLLLAFIEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           ALGFSSPLRNSIDAVSDRDFAMEFLSANSI AIHLSRLGEEWVLWSSEEFGFI P+DAVS
Sbjct: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPSDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKS RVIGDLVTLL+LCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           V GMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM
Sbjct: 361 VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKGLSLDDLRTLSPIIEKDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIA 479
           CLGKNCQLK LSL +LR LSPI ++DVYEFLGVENA++KFSSYGSTGSECVASQLEYWI 
Sbjct: 421 CLGKNCQLKDLSLAELRALSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLEYWIE 480

BLAST of Sgr023005 vs. TAIR 10
Match: AT5G10920.1 (L-Aspartase-like family protein )

HSP 1 Score: 721.1 bits (1860), Expect = 5.9e-208
Identity = 345/461 (74.84%), Postives = 407/461 (88.29%), Query Frame = 0

Query: 22  AKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDICGSRAHASMLAKQGLMSISDRDS 81
           +KE+KLWGGRF+ESVT+ VE+FTESIS+DK LYK DI GS+AHASMLA QGL++ SD+DS
Sbjct: 55  SKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSDKDS 114

Query: 82  ILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 141
           IL GLD+IER+I   +F WR DREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR
Sbjct: 115 ILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 174

Query: 142 LWCRDAIDSIVERIKDLQVAMVDLAIKNAGTIVPGYTHLQRAQPVLLQHLLLSFVEQVFS 201
           LWCRDAID+I+ +I++LQ A+V+LA+KN   IVPGYTHLQRAQPVLL H+LL+FVEQ+  
Sbjct: 175 LWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQLER 234

Query: 202 -----YYSRVRLNFCPLGACALAGTGLPIDRFMTSRALGFSSPLRNSIDAVSDRDFAMEF 261
                   R RLNF PLGACALAGTGLPIDRFMT+ ALGF+ P+RNSIDAVSDRDF +EF
Sbjct: 235 DAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFVLEF 294

Query: 262 LSANSITAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGR 321
           L  N+ T IHLSRLGEEWVLW+SEEFGF+TP+D+VSTGSSIMPQKKNPDPMELVRGKS R
Sbjct: 295 LYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGKSAR 354

Query: 322 VIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKK 381
           VIGDLVT+LTLCKGLPLAYNRD QEDKEP+FDS KT++GM++VSAEFA+N++FN+DRIKK
Sbjct: 355 VIGDLVTVLTLCKGLPLAYNRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDRIKK 414

Query: 382 ALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKGLSLDDLRTLSPIIE 441
           +LPAGHLDATTLADYLV KG+PFR+SHDIVGK V +C+ K C+L+ LSL++++ LSP+ E
Sbjct: 415 SLPAGHLDATTLADYLVKKGMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSPVFE 474

Query: 442 KDVYEFLGVENAISKFSSYGSTGSECVASQLEYWIAKLGIS 478
           +DV+ FLGVEN+++KFSSYGSTGS CVA QL YW+ KL I+
Sbjct: 475 EDVFGFLGVENSVNKFSSYGSTGSNCVAEQLGYWVNKLNIT 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131833.14.5e-25090.61argininosuccinate lyase, chloroplastic-like [Momordica charantia][more]
XP_038886091.11.9e-24890.37argininosuccinate lyase, chloroplastic-like [Benincasa hispida][more]
KAA0065095.12.6e-24589.26argininosuccinate lyase [Cucumis melo var. makuwa][more]
XP_023546913.14.4e-24589.71argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_004152599.17.4e-24589.46argininosuccinate lyase, chloroplastic [Cucumis sativus] >KGN62752.1 hypothetica... [more]
Match NameE-valueIdentityDescription
Q9LEU88.4e-20774.84Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10... [more]
Q6AR601.2e-12550.45Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) ... [more]
Q2LT965.2e-12451.10Argininosuccinate lyase OS=Syntrophus aciditrophicus (strain SB) OX=56780 GN=arg... [more]
Q3JDS26.8e-12451.54Argininosuccinate lyase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 ... [more]
Q2SQ672.6e-12352.37Argininosuccinate lyase OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=ar... [more]
Match NameE-valueIdentityDescription
A0A6J1BUL32.2e-25090.61argininosuccinate lyase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A5A7VGX51.2e-24589.26Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A0A0LPM33.6e-24589.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370510 PE=3 SV=1[more]
A0A1S3BBH83.6e-24589.05argininosuccinate lyase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103488... [more]
A0A6J1HD891.8e-24489.30argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G10920.15.9e-20874.84L-Aspartase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00145ARGSUCLYASEcoord: 212..228
score: 67.02
coord: 250..274
score: 52.15
coord: 125..147
score: 72.51
coord: 294..310
score: 81.13
coord: 329..348
score: 76.96
coord: 166..186
score: 61.86
NoneNo IPR availableGENE3D1.10.40.30coord: 379..451
e-value: 8.2E-176
score: 587.0
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 143..467
e-value: 8.2E-176
score: 587.0
NoneNo IPR availablePANTHERPTHR43814:SF4ARGININOSUCCINATE LYASEcoord: 37..477
IPR000362Fumarate lyase familyPRINTSPR00149FUMRATELYASEcoord: 250..277
score: 27.18
coord: 294..310
score: 64.09
coord: 171..189
score: 43.36
coord: 126..144
score: 38.14
IPR029419Argininosuccinate lyase, C-terminalPFAMPF14698ASL_C2coord: 384..449
e-value: 3.2E-20
score: 72.7
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 46..248
e-value: 8.2E-176
score: 587.0
IPR022761Fumarate lyase, N-terminalPFAMPF00206Lyase_1coord: 30..319
e-value: 4.2E-62
score: 210.3
IPR009049Argininosuccinate lyaseTIGRFAMTIGR00838TIGR00838coord: 27..469
e-value: 6.1E-165
score: 547.6
IPR009049Argininosuccinate lyasePANTHERPTHR43814ARGININOSUCCINATE LYASEcoord: 37..477
IPR009049Argininosuccinate lyaseHAMAPMF_00006Arg_succ_lyasecoord: 26..472
score: 38.429264
IPR009049Argininosuccinate lyaseCDDcd01359Argininosuccinate_lyasecoord: 46..474
e-value: 0.0
score: 631.889
IPR020557Fumarate lyase, conserved sitePROSITEPS00163FUMARATE_LYASEScoord: 294..303
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 27..472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr023005.1Sgr023005.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042450 arginine biosynthetic process via ornithine
cellular_component GO:0005829 cytosol
molecular_function GO:0004056 argininosuccinate lyase activity
molecular_function GO:0003824 catalytic activity