Sgr022935 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr022935
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptiontRNA (guanine-N(7)-)-methyltransferase
Locationtig00000729: 1134002 .. 1136197 (+)
RNA-Seq ExpressionSgr022935
SyntenySgr022935
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCATTACTCCGGCGGTTGTCTTCAGCATTTGGCGATACCTATTCGGTAAACTCTTCACGTATTCTGGTTTTGTTCTTCGTTTTAAGTTTTTTCCCGCCTTCTGAATTTTGTTCGCTTGATATCTTTGATGCGTAGCTAGTAGCTAGATTCGCGCAGTCCTTCGAGAGAGTCGTGGTTGATGGGCTGAGCTCGTTAATGTTTGTCTAATTAGTTTCTTTTTTGTCAATTGGGAATGTTTTTATGTTTTCTGATTCAAGTGTTATTGTTTTCAATTCGTTCCTGCTCTTGTGTTTGTTTGCGAAATACTTTACTTACTAATATGCAGACTAGGTGTTTGAAATAATTCCTTTGAATTTTTTTTACTTCCTGTTTCATATAATAGTTTGTCATTGTCAAGGCTTGCTTTTGACCCCTTTACATGCTTGTGATCAAGCGTTTTACCTCTTCAGATAAGTTTGGGTGTTGGATTTTCTTGGTGTGTTTTTCCAAATCACAGTCCCTTGATCTTCATTTCTCTAAAAGATGATGCAAGACACAGAGCCTTGCATATTCTTCTCCTGCTATTTGTTATTGTATGGAAAAATTAATGTTTGGAATTGGTTGCAATATACTGCCTCATGTGAGACTTGATAATTCTCTGTTGTGAAGTGCCAAAATACTAGCAGGTAGCAACTTAACTGTTTATAGCATTCTTATGTTATTATCTGCCACCTCTAAAGGGAATAAACTTTGAATTTTTGAATTATGTTGTATAAAATAGATTTTCAGCAGTTTATATATAGATCTTTTTGCATCTCAAGCTGGAAAGGCTTAGAAAATAAAGTTTCTTATATGCCTTGATTAAAATTTTCCTTGCATTTGGTCAGAAGAAAGAATTTTGATTGTTTACCTAATGTGGAATGAGGCCACCTTCTAATGAAATGGTTTGACCGATATTCTATATGTGATATTTACATAATTGGCCTTACTTTTTCATGATATACCTTTCGTTTGATCTGATGAAGACCCACTTAAGTTTTCTTTGTGATTATTTGAATTCCACTCATTCATTATATCCATGTTACAGTTAATAAATCCTGCATTATTTTTTACTTGTATTTCTCATGCATCAGGTGTTACCAGGAATCATTTCCTTGGAACTTCATCCTTTTACTTTACTTCCAATAAGAATTGTGATATTTATATCATGTACTCAACGAGTGCTTGTGGGGCTTAATCCTTCCTTGAAATCTTTGTAACTTAAGGCCTCAACTATTATTAGTGGGCCCATTCTGAACTCTTTAGATTTCATTCCAATGATTACATTTGTTTGACTGTGCTCACGGAGATTATCATTACAATTTTTATCTAAACTTTTTTTTTCATTGTAAGTCCCTCTTTGTAAGTACGATTTCTCCATTAACATATCTTTCCTGTGGTCTTGCAGCATTAAACATGTTGGAACATGATGCAAATCCAACTATTAGCAAGTCAACAGGCCTGCCACGGAAGCGTTTCTACCGTGCAAGAGCACACAGCAACCCTCTGAGTGACTCTCACTTCCCGGTACCAATATCCCCCAGTGAAGTTAATTATTCCCTTCATTACCCTCAACTGTTCCTTCAAATGATCAGCGAGATAGATCTAAAAAGATTGAATTTGCAGACATTGGTTGTGGTTTTGGAGGTCTTCTGATTAGTCTTTCAAACCTTTTCCCCGAAACCCTGATGATTGGAATGGAACTCAGGGATAAAGTAACAGAGTATGTCAAGGAACGGATTTTAGCTTTGAGAGTAGCAAATCCTGGCGAGTACCAAAACATCTCGGTGGTTCGAACAAACTCCATGAAATACATTCCAAACTACTTCGAAAAAGGGCAACTTTCGAAAATGTTTTTCTTGTTCCCCGACCCTCATTTCAAAGAGAAGAATCATCGCCGACGCGTGATCAGTCCATATTTGCTTGATGAGTATGCATATGCTCTGAGAGTTGGTGGGATTATATACACAATTACAGATGTGGAAGAGCTTGGAGAGTGGATGAAGACTTGTCTAGAGAATCACCCGTTGTTCGTTGCTCTAACGGCGGAAGAGCTCGAAGCAGATCCGGCTGTAAAGCTTTTAAGTTCTGCAACTGAGGAAGGACAAAAGGTTGCAAGAAATGAGGGACAGACTTTTCAGGCTGTTTATAGACGCATTAGTGCAGCACCATGA

mRNA sequence

ATGCCCATTACTCCGGCGGTTGTCTTCAGCATTTGGCGATACCTATTCGGTAAACTCTTCACGTATTCTGCATTAAACATGTTGGAACATGATGCAAATCCAACTATTAGCAAGTCAACAGGCCTGCCACGGAAGCGTTTCTACCGTGCAAGAGCACACAGCAACCCTCTGAGTGACTCTCACTTCCCGCGAGATAGATCTAAAAAGATTGAATTTGCAGACATTGGTTGTGGTTTTGGAGGTCTTCTGATTAGTCTTTCAAACCTTTTCCCCGAAACCCTGATGATTGGAATGGAACTCAGGGATAAAGTAACAGAGTATGTCAAGGAACGGATTTTAGCTTTGAGAGTAGCAAATCCTGGCGAGTACCAAAACATCTCGGTGGTTCGAACAAACTCCATGAAATACATTCCAAACTACTTCGAAAAAGGGCAACTTTCGAAAATGTTTTTCTTGTTCCCCGACCCTCATTTCAAAGAGAAGAATCATCGCCGACGCGTGATCAGTCCATATTTGCTTGATGAGTATGCATATGCTCTGAGAGTTGGTGGGATTATATACACAATTACAGATGTGGAAGAGCTTGGAGAGTGGATGAAGACTTGTCTAGAGAATCACCCGTTGTTCGTTGCTCTAACGGCGGAAGAGCTCGAAGCAGATCCGGCTGTAAAGCTTTTAAGTTCTGCAACTGAGGAAGGACAAAAGGTTGCAAGAAATGAGGGACAGACTTTTCAGGCTGTTTATAGACGCATTAGTGCAGCACCATGA

Coding sequence (CDS)

ATGCCCATTACTCCGGCGGTTGTCTTCAGCATTTGGCGATACCTATTCGGTAAACTCTTCACGTATTCTGCATTAAACATGTTGGAACATGATGCAAATCCAACTATTAGCAAGTCAACAGGCCTGCCACGGAAGCGTTTCTACCGTGCAAGAGCACACAGCAACCCTCTGAGTGACTCTCACTTCCCGCGAGATAGATCTAAAAAGATTGAATTTGCAGACATTGGTTGTGGTTTTGGAGGTCTTCTGATTAGTCTTTCAAACCTTTTCCCCGAAACCCTGATGATTGGAATGGAACTCAGGGATAAAGTAACAGAGTATGTCAAGGAACGGATTTTAGCTTTGAGAGTAGCAAATCCTGGCGAGTACCAAAACATCTCGGTGGTTCGAACAAACTCCATGAAATACATTCCAAACTACTTCGAAAAAGGGCAACTTTCGAAAATGTTTTTCTTGTTCCCCGACCCTCATTTCAAAGAGAAGAATCATCGCCGACGCGTGATCAGTCCATATTTGCTTGATGAGTATGCATATGCTCTGAGAGTTGGTGGGATTATATACACAATTACAGATGTGGAAGAGCTTGGAGAGTGGATGAAGACTTGTCTAGAGAATCACCCGTTGTTCGTTGCTCTAACGGCGGAAGAGCTCGAAGCAGATCCGGCTGTAAAGCTTTTAAGTTCTGCAACTGAGGAAGGACAAAAGGTTGCAAGAAATGAGGGACAGACTTTTCAGGCTGTTTATAGACGCATTAGTGCAGCACCATGA

Protein sequence

MPITPAVVFSIWRYLFGKLFTYSALNMLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPRDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEYQNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVGGIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRISAAP
Homology
BLAST of Sgr022935 vs. NCBI nr
Match: KAG7020318.1 (tRNA (guanine-N(7)-)-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 431.4 bits (1108), Expect = 5.4e-117
Identity = 220/258 (85.27%), Postives = 225/258 (87.21%), Query Frame = 0

Query: 21  TYSALNMLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP----------------- 80
           T  +LNMLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                 
Sbjct: 16  TSISLNMLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQL 75

Query: 81  ------RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRV 140
                   RSKKIEFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRV
Sbjct: 76  FPSNDQLHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRV 135

Query: 141 ANPGEYQNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYA 200
           ANPG+YQN+SVVRTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISP+LLDEYA
Sbjct: 136 ANPGQYQNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA 195

Query: 201 YALRVGGIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVA 256
           Y LR GGIIYTITDVEELGEWMK+CLENHPLF ALTAEELE DPAVKLLSSATEEGQKVA
Sbjct: 196 YTLRAGGIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVA 255

BLAST of Sgr022935 vs. NCBI nr
Match: XP_022132320.1 (tRNA (guanine-N(7)-)-methyltransferase [Momordica charantia] >XP_022132322.1 tRNA (guanine-N(7)-)-methyltransferase [Momordica charantia])

HSP 1 Score: 431.4 bits (1108), Expect = 5.4e-117
Identity = 219/252 (86.90%), Postives = 225/252 (89.29%), Query Frame = 0

Query: 27  MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP----------------------- 86
           MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                       
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60

Query: 87  RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEY 146
            DRSKK+EFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPG+Y
Sbjct: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 147 QNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG 206
           QNISVVRTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISP+LLDEYA+ALRVG
Sbjct: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180

Query: 207 GIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQT 256
           GIIYTITDVEELGEWMK CLENHPLF ALTAEELEADPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240

BLAST of Sgr022935 vs. NCBI nr
Match: XP_031736486.1 (tRNA (guanine-N(7)-)-methyltransferase isoform X1 [Cucumis sativus] >XP_031736487.1 tRNA (guanine-N(7)-)-methyltransferase isoform X1 [Cucumis sativus])

HSP 1 Score: 430.3 bits (1105), Expect = 1.2e-116
Identity = 219/254 (86.22%), Postives = 226/254 (88.98%), Query Frame = 0

Query: 25  LNMLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP--------------------- 84
           L MLE++ANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                     
Sbjct: 4   LTMLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPISPSEVDYSLHYPQLFPSN 63

Query: 85  --RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPG 144
             RD++KKIEFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPG
Sbjct: 64  DQRDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPG 123

Query: 145 EYQNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALR 204
           +YQNISV RTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALR
Sbjct: 124 QYQNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALR 183

Query: 205 VGGIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEG 256
           VGGIIYTITDVEELGEWMKTCLENHPLF ALT+EELEADPAVKLLSSATEEGQKVARNEG
Sbjct: 184 VGGIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEG 243

BLAST of Sgr022935 vs. NCBI nr
Match: XP_038884381.1 (tRNA (guanine-N(7)-)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 429.1 bits (1102), Expect = 2.7e-116
Identity = 220/254 (86.61%), Postives = 224/254 (88.19%), Query Frame = 0

Query: 25  LNMLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP--------------------- 84
           L MLE+DANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                     
Sbjct: 7   LTMLENDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQLFPSN 66

Query: 85  --RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPG 144
             RDRSKKIEFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPG
Sbjct: 67  DQRDRSKKIEFADIGCGFGGLLISLSALFPETLMIGMELRDKVTEYVKERILALRVANPG 126

Query: 145 EYQNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALR 204
           +YQNISV RTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISP+LLDEYAYALR
Sbjct: 127 QYQNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYALR 186

Query: 205 VGGIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEG 256
           VGGIIYTITDVEELG+WMKTCLENHPLF ALT EELEADPAVKLL SATEEGQKVARNEG
Sbjct: 187 VGGIIYTITDVEELGDWMKTCLENHPLFEALTLEELEADPAVKLLCSATEEGQKVARNEG 246

BLAST of Sgr022935 vs. NCBI nr
Match: XP_004138787.1 (tRNA (guanine-N(7)-)-methyltransferase isoform X2 [Cucumis sativus] >XP_031736488.1 tRNA (guanine-N(7)-)-methyltransferase isoform X2 [Cucumis sativus] >KGN62840.1 hypothetical protein Csa_022503 [Cucumis sativus])

HSP 1 Score: 428.7 bits (1101), Expect = 3.5e-116
Identity = 218/252 (86.51%), Postives = 225/252 (89.29%), Query Frame = 0

Query: 27  MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP----------------------- 86
           MLE++ANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                       
Sbjct: 1   MLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPISPSEVDYSLHYPQLFPSNDQ 60

Query: 87  RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEY 146
           RD++KKIEFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPG+Y
Sbjct: 61  RDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 147 QNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG 206
           QNISV RTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG
Sbjct: 121 QNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG 180

Query: 207 GIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQT 256
           GIIYTITDVEELGEWMKTCLENHPLF ALT+EELEADPAVKLLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEGQT 240

BLAST of Sgr022935 vs. ExPASy Swiss-Prot
Match: Q8GXB7 (tRNA (guanine-N(7)-)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=At5g24840 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.0e-99
Identity = 185/240 (77.08%), Postives = 201/240 (83.75%), Query Frame = 0

Query: 35  TISKSTGLPRKRFYRARAHSNPLSDSHFPRDRS---------------------KKIEFA 94
           T SKSTGLPRKRFYRARAHSNPLSDSHFP   S                     KK+EFA
Sbjct: 9   TFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKFIKKVEFA 68

Query: 95  DIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILAL-RVANPGEYQNISVVRTN 154
           DIGCGFGGLLISL+ LFP+TLMIGMELRDKVTEYVKERILAL R ++ G+Y+NISVVRTN
Sbjct: 69  DIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQYENISVVRTN 128

Query: 155 SMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVGGIIYTITDV 214
           SMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVIS +LLDEYAY LR GGIIYTITDV
Sbjct: 129 SMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTITDV 188

Query: 215 EELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIS 253
           EELGEWMK+CLE HP+F +LT EEL +DP V+LL SATEEGQKVARN GQTF+AV+RRI+
Sbjct: 189 EELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQTFRAVFRRIA 248

BLAST of Sgr022935 vs. ExPASy Swiss-Prot
Match: A2YB34 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN=OsI_22311 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.3e-94
Identity = 175/239 (73.22%), Postives = 189/239 (79.08%), Query Frame = 0

Query: 42  LPRKRFYRARAHSNPLSDSHFPRDRS----------------------------KKIEFA 101
           LPRKRFYRARAHSNPLSDSHFP   S                             +I FA
Sbjct: 18  LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77

Query: 102 DIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEYQNISVVRTNS 161
           D+GCGFGGLL+ LS LFP+TLMIGMELRDKVTEYVKERILALR +NPG+Y NISVVRTNS
Sbjct: 78  DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137

Query: 162 MKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVGGIIYTITDVE 221
           MKYIPNYF K QLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYAY + VGGIIYTITDVE
Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197

Query: 222 ELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIS 253
           ELGEWM++CLE HPLF A+  EE +ADP VKLLS+ATEEGQKVARN GQTFQA++RRIS
Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256

BLAST of Sgr022935 vs. ExPASy Swiss-Prot
Match: Q67V78 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0236900 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.3e-94
Identity = 175/239 (73.22%), Postives = 189/239 (79.08%), Query Frame = 0

Query: 42  LPRKRFYRARAHSNPLSDSHFPRDRS----------------------------KKIEFA 101
           LPRKRFYRARAHSNPLSDSHFP   S                             +I FA
Sbjct: 18  LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77

Query: 102 DIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEYQNISVVRTNS 161
           D+GCGFGGLL+ LS LFP+TLMIGMELRDKVTEYVKERILALR +NPG+Y NISVVRTNS
Sbjct: 78  DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137

Query: 162 MKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVGGIIYTITDVE 221
           MKYIPNYF K QLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYAY + VGGIIYTITDVE
Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197

Query: 222 ELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIS 253
           ELGEWM++CLE HPLF A+  EE +ADP VKLLS+ATEEGQKVARN GQTFQA++RRIS
Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256

BLAST of Sgr022935 vs. ExPASy Swiss-Prot
Match: B6SHG7 (tRNA (guanine-N(7)-)-methyltransferase OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 8.1e-92
Identity = 171/239 (71.55%), Postives = 187/239 (78.24%), Query Frame = 0

Query: 42  LPRKRFYRARAHSNPLSDSHFPRDRS----------------------------KKIEFA 101
           LPRKRFYRARAHSNPLSDSHFP   S                            ++I FA
Sbjct: 13  LPRKRFYRARAHSNPLSDSHFPVPISPEEVDLSQHYPRYFPADKGSDGEEAAAPQQIRFA 72

Query: 102 DIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEYQNISVVRTNS 161
           D+GCGFGGLL+ LS LFP+TLMIGMELRDKVTEYVKERILALR ANPG+Y NISVVRTNS
Sbjct: 73  DVGCGFGGLLVGLSPLFPDTLMIGMELRDKVTEYVKERILALRGANPGQYDNISVVRTNS 132

Query: 162 MKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVGGIIYTITDVE 221
           MKYIPNYF K QL+KMFFLFPDPHFKEKNHRRRVIS  LLDEYAY + VGGIIYTITDVE
Sbjct: 133 MKYIPNYFRKAQLTKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 192

Query: 222 ELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIS 253
           ELGEWM++CLE HPLF  +  EE++ADP  KLLS+ATEE QKVARN GQTF A++RRIS
Sbjct: 193 ELGEWMRSCLEKHPLFETVPEEEIKADPVFKLLSTATEESQKVARNGGQTFYAIFRRIS 251

BLAST of Sgr022935 vs. ExPASy Swiss-Prot
Match: Q23126 (tRNA (guanine-N(7)-)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=metl-1 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-70
Identity = 135/246 (54.88%), Postives = 174/246 (70.73%), Query Frame = 0

Query: 24  ALNMLEHDANPTISKSTGLPRKRFYRARAHSNPLSDS--HFP--------------RDRS 83
           AL  +E D  PT     GLP+K+ YR RAHSNP SD    +P                + 
Sbjct: 6   ALTEMELDHKPTCETVPGLPQKKHYRQRAHSNPHSDHDIEYPLTPNHMDWTKYYGDYTKG 65

Query: 84  KKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRV--ANPGEYQN 143
           ++++FADIGCG+GGLL+ LS  +P+ LMIGME+R KV++YV E+I ALR   A  G Y+N
Sbjct: 66  RQVDFADIGCGYGGLLMRLSPKYPDNLMIGMEIRVKVSDYVNEKIQALRKHHAEAGHYRN 125

Query: 144 ISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVGGI 203
           ++V+R+N+MKY+PNYF KGQLSKMFFLFPDPHFK K H+ R+I+P LL EYAY LR GGI
Sbjct: 126 VAVLRSNAMKYMPNYFHKGQLSKMFFLFPDPHFKNKKHKWRIITPTLLSEYAYVLREGGI 185

Query: 204 IYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQTFQ 252
           IYTITDV++L EWM   L  HPLF  LT EE++ DP V++L  +TEEGQKV RN+G  + 
Sbjct: 186 IYTITDVKDLHEWMVKHLSEHPLFERLTEEEMKKDPIVEMLFESTEEGQKVTRNDGGKWP 245

BLAST of Sgr022935 vs. ExPASy TrEMBL
Match: A0A6J1BTJ1 (tRNA (guanine-N(7)-)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111005201 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 2.6e-117
Identity = 219/252 (86.90%), Postives = 225/252 (89.29%), Query Frame = 0

Query: 27  MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP----------------------- 86
           MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                       
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60

Query: 87  RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEY 146
            DRSKK+EFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPG+Y
Sbjct: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 147 QNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG 206
           QNISVVRTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISP+LLDEYA+ALRVG
Sbjct: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180

Query: 207 GIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQT 256
           GIIYTITDVEELGEWMK CLENHPLF ALTAEELEADPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240

BLAST of Sgr022935 vs. ExPASy TrEMBL
Match: A0A0A0LLM3 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G376290 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.7e-116
Identity = 218/252 (86.51%), Postives = 225/252 (89.29%), Query Frame = 0

Query: 27  MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP----------------------- 86
           MLE++ANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                       
Sbjct: 1   MLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPISPSEVDYSLHYPQLFPSNDQ 60

Query: 87  RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEY 146
           RD++KKIEFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPG+Y
Sbjct: 61  RDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 147 QNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG 206
           QNISV RTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG
Sbjct: 121 QNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG 180

Query: 207 GIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQT 256
           GIIYTITDVEELGEWMKTCLENHPLF ALT+EELEADPAVKLLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEGQT 240

BLAST of Sgr022935 vs. ExPASy TrEMBL
Match: A0A1S4DVC5 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103488206 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.2e-116
Identity = 218/252 (86.51%), Postives = 225/252 (89.29%), Query Frame = 0

Query: 27  MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP----------------------- 86
           MLE++ANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                       
Sbjct: 1   MLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQLFPSNDQ 60

Query: 87  RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEY 146
           RD++KKIEFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPG+Y
Sbjct: 61  RDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 147 QNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG 206
           QNISV RTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISP+LLDEYAYALRVG
Sbjct: 121 QNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYALRVG 180

Query: 207 GIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQT 256
           GIIYTITDVEELGEWMKTCLENHPLF ALT+EELEADPAVKLLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEGQT 240

BLAST of Sgr022935 vs. ExPASy TrEMBL
Match: A0A5A7VCU7 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003450 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.2e-116
Identity = 218/252 (86.51%), Postives = 225/252 (89.29%), Query Frame = 0

Query: 27  MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP----------------------- 86
           MLE++ANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                       
Sbjct: 1   MLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQLFPSNDQ 60

Query: 87  RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEY 146
           RD++KKIEFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPG+Y
Sbjct: 61  RDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 147 QNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG 206
           QNISV RTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISP+LLDEYAYALRVG
Sbjct: 121 QNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYALRVG 180

Query: 207 GIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQT 256
           GIIYTITDVEELGEWMKTCLENHPLF ALT+EELEADPAVKLLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEGQT 240

BLAST of Sgr022935 vs. ExPASy TrEMBL
Match: A0A6J1KTN7 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111496303 PE=3 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.4e-115
Identity = 216/252 (85.71%), Postives = 221/252 (87.70%), Query Frame = 0

Query: 27  MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP----------------------- 86
           MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP                       
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60

Query: 87  RDRSKKIEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGEY 146
             R KKIEFADIGCGFGGLLISLS +FPETLMIGMELRDKVTEYVKERILALRVANPG+Y
Sbjct: 61  LHRPKKIEFADIGCGFGGLLISLSTIFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 147 QNISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVG 206
           QN+SVVRTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVISP+LLDEYAY LRVG
Sbjct: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180

Query: 207 GIIYTITDVEELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQT 256
           GIIYTITDVEELGEWMK+CLENHPLF ALTAEELE DPAVKLLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240

BLAST of Sgr022935 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 362.5 bits (929), Expect = 2.9e-100
Identity = 185/240 (77.08%), Postives = 201/240 (83.75%), Query Frame = 0

Query: 35  TISKSTGLPRKRFYRARAHSNPLSDSHFPRDRS---------------------KKIEFA 94
           T SKSTGLPRKRFYRARAHSNPLSDSHFP   S                     KK+EFA
Sbjct: 9   TFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKFIKKVEFA 68

Query: 95  DIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILAL-RVANPGEYQNISVVRTN 154
           DIGCGFGGLLISL+ LFP+TLMIGMELRDKVTEYVKERILAL R ++ G+Y+NISVVRTN
Sbjct: 69  DIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQYENISVVRTN 128

Query: 155 SMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVGGIIYTITDV 214
           SMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVIS +LLDEYAY LR GGIIYTITDV
Sbjct: 129 SMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTITDV 188

Query: 215 EELGEWMKTCLENHPLFVALTAEELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIS 253
           EELGEWMK+CLE HP+F +LT EEL +DP V+LL SATEEGQKVARN GQTF+AV+RRI+
Sbjct: 189 EELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQTFRAVFRRIA 248

BLAST of Sgr022935 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 59.3 bits (142), Expect = 5.2e-09
Identity = 53/194 (27.32%), Postives = 88/194 (45.36%), Query Frame = 0

Query: 25  LNMLEHDANPTISKSTGLPRKRFYRARAHSNPLSDS-----------HFPRDRSKKIEFA 84
           L   E + N  IS+  G       R R H NPLS S              +D S  +   
Sbjct: 76  LEYAELNLNHKISEEVG-----HVRIRQHVNPLSSSFSKPAPVPVWDEVYKDPSLPL-MV 135

Query: 85  DIGCGFGGLLISLSNLFPETL-MIGMELRDKVTEYVKERILALRVANPGEYQNISVVRTN 144
           DIG G G  L+  +N   E+   +G+E+R K+ +     +  L ++      N+  +  N
Sbjct: 136 DIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGLS------NVHFIFAN 195

Query: 145 SMKYIPNYFEK--GQLSKMFFLFPDPHFKEKNHRRRVISPYLLDEYAYALRVGGIIYTIT 204
           +M    +      G L  +  L PDPHFK+++ +RRV+   L++     L+ GG I+  +
Sbjct: 196 AMVSFEHLISSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQNLKPGGKIFVQS 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7020318.15.4e-11785.27tRNA (guanine-N(7)-)-methyltransferase, partial [Cucurbita argyrosperma subsp. a... [more]
XP_022132320.15.4e-11786.90tRNA (guanine-N(7)-)-methyltransferase [Momordica charantia] >XP_022132322.1 tRN... [more]
XP_031736486.11.2e-11686.22tRNA (guanine-N(7)-)-methyltransferase isoform X1 [Cucumis sativus] >XP_03173648... [more]
XP_038884381.12.7e-11686.61tRNA (guanine-N(7)-)-methyltransferase isoform X1 [Benincasa hispida][more]
XP_004138787.13.5e-11686.51tRNA (guanine-N(7)-)-methyltransferase isoform X2 [Cucumis sativus] >XP_03173648... [more]
Match NameE-valueIdentityDescription
Q8GXB74.0e-9977.08tRNA (guanine-N(7)-)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=At5g24... [more]
A2YB342.3e-9473.22tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN... [more]
Q67V782.3e-9473.22tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 ... [more]
B6SHG78.1e-9271.55tRNA (guanine-N(7)-)-methyltransferase OS=Zea mays OX=4577 PE=2 SV=1[more]
Q231261.3e-7054.88tRNA (guanine-N(7)-)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=metl... [more]
Match NameE-valueIdentityDescription
A0A6J1BTJ12.6e-11786.90tRNA (guanine-N(7)-)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A0A0LLM31.7e-11686.51tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G37629... [more]
A0A1S4DVC52.2e-11686.51tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103488206 P... [more]
A0A5A7VCU72.2e-11686.51tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1KTN71.4e-11585.71tRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114963... [more]
Match NameE-valueIdentityDescription
AT5G24840.12.9e-10077.08tRNA (guanine-N-7) methyltransferase [more]
AT5G17660.15.2e-0927.32tRNA (guanine-N-7) methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePFAMPF02390Methyltransf_4coord: 70..245
e-value: 2.0E-51
score: 173.9
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typeTIGRFAMTIGR00091TIGR00091coord: 69..251
e-value: 6.7E-43
score: 144.3
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePANTHERPTHR234173-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASEcoord: 36..252
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePROSITEPS51625SAM_MT_TRMBcoord: 30..250
score: 50.574047
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 42..250
e-value: 1.1E-79
score: 268.9
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 72..189
e-value: 2.41324E-4
score: 37.7947
IPR025763tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryotePANTHERPTHR23417:SF16TRNA (GUANINE-N(7)-)-METHYLTRANSFERASEcoord: 36..252
IPR025763tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryoteHAMAPMF_03055tRNA_methyltr_TrmB_eukcoord: 42..251
score: 40.913696
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 67..216

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr022935.1Sgr022935.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0106004 tRNA (guanine-N7)-methylation
biological_process GO:0006400 tRNA modification
cellular_component GO:0005634 nucleus
cellular_component GO:0043527 tRNA methyltransferase complex
molecular_function GO:0000049 tRNA binding
molecular_function GO:0008176 tRNA (guanine-N7-)-methyltransferase activity