Sgr022874 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr022874
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionGDSL esterase/lipase
Locationtig00000589: 2708184 .. 2715615 (-)
RNA-Seq ExpressionSgr022874
SyntenySgr022874
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCAAAAAGCTCTTCTTCTACTTCTTCTTCTCTCTCCTCCCAGGTTTCCCTCTTCCCCACTTTCTCTCTCTAAACCCCCCTCCCCCCCTCTCTCTCTCTCTCTATATCATCGGTGTTTTTTTTTTTGTTTTGTTTTGGGTTTTTGTTGCAGAACTATACGGGGTGGTTCAGGTTTCAGCTGCCCACGGCGGCCACCACCGTCTCCGGCGGAGCAGCCTGATCCTCGGAGCAGGCCAGCTCTCGCCCGGTTCGCAGCCCAGAAAGCTGTTTGTGTTTGGGGACTCGTACGTCGACACCGGAAACAACCGGAAACCGGCGGCGAAATCTTGGCAGTACCCCTACGGAATTACCTTCCCCGGAAAACCCACCGGCCGTTTCTCCGATGGCCGTGTTTTGACCGATTACCTAGGTAACCCTGAAAAACCACCACCTCTCTCTCTCTCTCTTGCTCTTTTTCTTTTTGTGTACTTTGAATTATTGTTTCAATTTCCTCACCTTTATGTTTGAATCTTCGTCTAATTTGGGTAATTATGTTGTTTTTAATTATATGTTTTTAAAAAATTATTTAATTATCAATTCAAACATAACTCATTGGATAAGATACTTAAAACTGTCTCAAAATTCGATGCTTCAATCTCCATCTCTTAATTGGTAAACTAAAAAAAAAAAAATTGTTTAATTAGCAACTTTAATTATGTAATATACGTTTGAAATCTTAGACAGAATTGGAAAAATTAGAGGTTAAATTAGCAGAGATGAAGGGCTAGTTTGATAATAAACTAGTTAATTAATTACTTTAGTTGTTTTTATAGCTATTTTATTTAAATTTTATGGTTTAAAAATTAAAATTGTTTACTTACGAACAATTTGATAATAAATAGTTTGTAATCTACGTTTTAACTTATTTTGTTTTTTGATTTCATAATTTTGTATAAAATTGATTTTACTTACTAATAATATCTTCCATGTTCTGTGATCTGTTATATGAAAATATTTTTACAATCTTAGAGAAATTTTAAAATTAAATAAATGGTTTTTATTTTTAAAATTAAATTAAATAAGACTTCATTTCATATCGATTTCATTTTTGTCTTAAAAATAGACCTTCGTTTGCTGCCTTAAATTTTCAAGACCCTTTCGAAATTTTAGTCCAACTTAAAAAATCAATAATTTTTTTTTTTGGTCAATGTTTTAAATTTAGCTCTAAACTCTAAAAATATATATTTTTAAATATGTAAAATATAGAATAAAGAAGTTGTCAATAAAAAAATTTAATTTTTAGATATAGAAAACAAGAAAAAAAAATCATAAGTTAACAAATTAAATTTAAAATAGAAAACGAGAAACAAAATATCATAATCAAATGGATCTAAAAACAAGATTAAAGAAATGAATAAGCATCTTTATATTTTAAAAACAAAAAGAATGAAAATGTTAGAAAAGGACAGCATGGTATGATTAAAGATGGGTCTTAAAACAAAATATAATATTCAAAAGGCTTGGAAAAAAAAAAAAGATAAATGAGCTTTATTTTTAAGGCCACATGTCTTTAGCTTTAGTTTTATGGACACAGAGTGTAGAAGAAAGAACTTGAGAATTGCTTTAAGAAATGAAATATACTTAAAAAATATATATATATCAATTGAGGTGGTGGGGTTCCTCATTGCTCTTATATTAGCTTTTTTTTTTTTGTTTAAATATCATTTTGGTCTCTGAACTTTCAAATTTTTTCTATTTTTGTCTCTGAACTTTCAAAATGTCTATTTTGGTCCCTGAACTTTCAATTTTGTTTTATTTTGGTCCTTGAACTTTCAAAATGTCTATTTTGGTCCCTAAACTTTCCAAAAATAATCATTTTGGTCTTTAATATATTATTTTGACTCCTAATATCTACTTGATTAATACTTACCTCCAACCTATATGACTTGAATACATGCTTACTTTAACATGTTAATACATATACTCAAGGCTATGTCATTAATTTGTTAAAAATAAAATAATAGTAAGGACCAAAATAGTTATTTTTGGAAAGTTCAGGGACTAAAATAGACATTTTGAAAGTTTAGGGACCAAAATAGAACAAACTTGAAGGTTCAGGGACCAAAATGGACATTTTAAAAATTCAGGGACCAAAATGGAAAAAACTTGAAAGTTCAGGGACCAAAATGATATTTAAACCTTTTTTTTTCTCCTTTTTATTTTAAGTTTAAATCTAATTTTTATGTCTAAAATTTTAAAATATCTATTTTTATCTTTAAATTTTAAAAAAATAACTATTTTTATTTTTACGATTATTATATCACATAATATGAACCTACCTAATACCTACTTTAGCCAATTTATGTGATTCTGAGACGTGCCTATATCAGTACGTTAATATACATATTTAAAAGTTATATTATCCATATGTTAAAGCAAAATAACGATGAAGACTAAAATATAATAAATTTAAAAATTTAAAGATTAAAATAAATGATTTAATAGGGTAAAAAAAATAAACTTTCTTTTTTTTAATTTCAATATATGGAAATGACAATTGCCAAGTCAGAGAGAGGTGCTTTTGGCTGTAAAATCAAGAATGGATGCATTATTGAGTCAGCTTTTTTTTTTTTTTTTGGTGGTATGGGGATGGCTGTGTCTTTTTCATATGTCAGTTTAGCATTACCCTTTTTTTTTTTTCCTGACAGACAGTCTTACTTTCTCACATGATCTCAAGGATTTGGACCCATCTTCAATTTCACTACGTTTACCTCATCATCTTCCCCTTTGTTTTTTTCATTCTCATTCTCCCAATTACCTCTCTTTCTTTTTAAAATATTACAAATCAAATCAAACGAGGACTAGTAGTACCCTTCACCTATACGAAAGGTTATTAATATTACGATCAATTGAACTATGTTTAGACTTATCCTATGCTTTTTGGATGTCAATAAAATATTGATATTTTCCATGATTTGATCGATTTCATTCCAATTGATTATAAACTTGTTTCGATTAGTTTGAATTTAAATATAGTGAAATCGTGGGATTGAATTAGGATGTGTTTGAGAGTGTTTTTTGATGAAGTACTTTAATAAAAAATATTTAGTAACAAATCACTTTAAAGTTTGGTTTTACATTCTTAAAAGTATTTTTAAATCACTTTCAATCGGGCACCAGACCAAAATACTCTACAAGTACTTTGGACTTTGTAAAAGTACTTTTGTGTTCCTCTCTCAAATATGATGCCCGTAGTTTATGGGTTTTGTTAAAATATTGGATTGCATCAATTCAAATTTACTAATTAAAAAACATTATTGTGCATGTGTATCTCTTATGATTAAAATAGTATAGTAACAAGAACTGTGGTAAATAGCTATAATTCTTTTTTTAAAAATTTAGAAATTTGACTAAATCAGTATATGAATTTACTATTTAAATTTAGGATTTAATTTAAAAAGTTTTTAAAGTTAAAATTGAGATTATAGAGATAAAAAAAAAAACAATTTTCTCTAAGAAAAAACTAGATTTAAATCTCATTTTGGTCTCTGAACTTTTAATTTTATTCTATTTTGGTCCTTAAACTTTCAAAATGTCTATTTTAGTACCTGAACTTTCAAGTTTGTTATATTTTGGTCCATGAACTTTCAAAATGTTCCTGAATTTTCAAAAAATAACTATTTTGGTCATTGATATATTATTTTGACTCCTAATATCTACTGGATCAATGCTCTCTTCCAACCAATATGACTTGAATATATACTCATTTTAACATGTTAATACATATAATTAAGGCTGTGTCATTAATTTATTAAAAATAAAATAAATGTAAGAACTAAAATAATTATTTTTAAAAAGTTCGAATACAAAAATAAATATTTTAAAAATGGTTTAAATATCATTTTGGTCCTTGAACTTTCAAGTTTTTTTCATTTTGGTCCTTAAACTTTCAAAATGTCCATTTTGGTCCCTGAACTTTCAAGTTTGTTCTATTTTGGTCCCTGAACTTTCAAAATGTCTATTTTGGTCCCTGAACTTTTCAAAAATAATTATTTTGGTCCTTAATATATTATTTTAACTCCTAATATTTACTTGATTGATACTTACCTCCAAGCTCTATGACTTGAATACATGCTTACTTTAACATGCTAATACATATACCCAAGGTTATGTCATTAATTTATTAAAAATAAAATAAATGTAAGGACCAAAATAGTTATTTTTGGAAAGTTCAAGGACTAAAATAGACATTTTGAAAGTTCAGGGACCAAAATAGACATTTTGAAAGTTCAGGGACCAAAATGAAAAGAACTTGAAAGTTCAGGGACCAAAATGATATTTAAATCTTTAAAAATTTAGTAATCAAAATTAAATAAACTTGAAAATTCAGACAACGCTAGAATTTAAACCAAAAAACTAACAGCCCCTTCTAAGTTTTAACTATTACTTCCCCTTTTCTTCATCGGCACTCAAAATTTTCCCAATTCTCTTCTCCAAGCCGAAGATGGAAAAAGTTCAATCAAAATCAGCTCTAATTTGTTGCGCAAATGGCTGATTGAATCTTCTGTGGAAATGCAGCTAAGTTTCTGGGAGTGAAGTCTCCGATTCCGTACAAATGGCGGAAAGTAGGGTTCCGGCAGCTGAAATACGGGATGAACTTTGCGTACGGCGGCACCGGCGTGTTCGATACCTTCATTTTGAGCCCAAACATGAGCACTCAGATCGACTTCCTCCGGCAGCTCATCGACGAGTCCACCTTCACCAGCCAAGACTTGCAGTCCTCGGTGGCGCTCATCTCGCTCGCCGGCAACGACTATGGTGTCTATGAAGCCAGAAATGGCTCCACTGAGGTTTCCCTAAACTTTCTCTTTCATCTATAAACCCTAATTTGAAATGCTCTGTTTTTTAATTTTAAGAATTGTTTGTAAATCTCTTTCTTTTTTTTTTTTTTTTAATGATTGTCTTTTCATTTATTTTTTGGATTTAAAAATAAAACAAAGTTACTTGAAAATTAGACATATTTGAATCAACAAAAATACTAATTCTTTTTCGCCTATAAACTTTTTTAAAACATTATTCCCAATAATTTTTGAATAGATATGCAGTGTTTTTATTATTTTTGGTAGTACATAATAGAGATTACTACAAATTATTGGTTTAATTCCTAGTTTGATCCCTAATTCTTCATACTTATTTCTTTTTAGTTATTAAACTTTTAAATGTTCTATTTTAGCTCTCAACTCTCAAATATTTTAATTTAGCTCTCATTTTTTTATTGAAAAAATTATTTTAGTCGTTGTTAATTTTTTATTAATAATTTTAGAATCTATTTTGAACTTCGACATTCTTCATGCTACCTGTTATCCACTTAACTATCTAATCATCAAATAAATGAGCAAAAGACATATATATGTTAGGCTGGGGTGGAGCGAAAAAGGAAAGAAGGAGTTCCATAGTTCATCTTAGATTTAAAAAATGATATTAAAAAATTAAGATTTTAAAGTTTCATCTCTAATTCGCACCCTTAAATTTTCAAAATTGAAAAATGCTGTAATGTAAAATTTTATCTCTCCCTATAAAAATTTTTGCTCTGCCCATGATGTTAGGCATCTAGTAAACAATTCATCAAAAAATAAAATGTTTTTCTATTCAAAGTTGTAAGAATGGTTAAAAGTTTAGAGACCATTAATGCTTTCGTTTCAAAAAATAATAATAATAATGCCTTTGTATTGTAAAAATGATATCAAACATCTGCCCATTCATAAAATCTGCAAATCATATCAATTCTATTAAAAATAACGACAAGCTTTAACATAAAACATATGAGTTGTAGTTAGAAACATGTTTGAAAGGGGAGGATATGATTTACAAATTTCACGATCTATGCGTACAATGATTACCCGAGAAACGTTTTCAAAAGAGAAGGGTATGATTTGCAAATTTGGCAATCTCACTTGGTGTATTTTGCAAAATGTCCCCAAATATATAAACAGGTTTATGTTGAACTTTGGCATGTCGATGGCAGGGTTGGCAGCCATTTATAACCAAAGTGGTGAACCAGCTGGAGTTGAACCTGAGGCGCATCCATGGGCTGGGGATTCCAAAGATAGTGGTGACGGCTCTGGAGCCACTGGGCTGCCTTCCCAGCAGCACAGTCGCGTCCTCCTTCCAGCAGTGTAATGAAACTCAGAATCTGCTCGTCAACTTCCACAATCTGCTGCTGCAGCAAGCTGTGGCAAAACTCAACAGTGAAGTCAAAGATGACTCTGCTTCTACTTTCATCCTTCTTGATCTCTACTTCTCTTTCATGGCTGCTCTCAACAACAAAGGAGACCATCTTGGTGAGACTCTCTCTCTCTCTCTCAATCTTTTTTAGTTCATGTTTGAAGCCACAAATTCTATGTGCTACGTATATAACTTTGGTTCTATTTTGTTCTGCATGAGGATTGATCTTCCTCAACTAAACACTCCTGATTTACACTGTTGGGTGATGAGGAAATAGGCTGATGTTTAGCATATTAGAGACTATAGCCCTACTAGAGTGTCCGAGTTCTCTTGAAGAGGCGGAAGAAGTACGGGTGTGCATCTGCTTGTTTATGTTGTTGGGCCCACTGCCCTTATCCACTCAAATTCGAAAATGAAAGGAGTCATTCTCATTTGCTATTTGTAACCACCATGGATTGGCCTAGTGGTTTTTGGGGCCAATGAAAGAAATAAAGGACTTAGAGAGAATGGGTTCAAACCATGGTACCTACCTACTTAGAATTTAATATTCTACGACTTTATTTGACAACCAAATGTAATAGGGTTAGGCGGTTATCCTGTGAGATTAGTCAATGTGCACTTAAGCTGGCCCAAACACTCACAAATATTTTTTTTTTAAAAATCTTCATTCCCTATTTGTTCTAGGTGGTGTTCTTGGTCACTATGAAAGCTCCCCACTTCGTAAATTGCACTCGCTACCTCTCGAGTCTCGTCTGCAGTCATGGTATAGGTAGAGTTTCTTGCCTTCCTTCCCAAGTCCAAAATAGGGTAGCTAGTGTTCTTAAACAAACGACTTGAAACACTTCTCCGTAAGAAGCATCCTTGGATTGGTTGCAACCACACTTAGTCTTAGTGTAAGAATCCAATATTCACAGGCTTCCCATAATCTCTTAGAAAGTAAATGATCGTGTACGTATGCAACGGGTCACACCGAACCCAAAAGTAACGATGCAGTTGGGAATTTTTTTGTGCAGGGAATGTGAAGTTTGAGAACCCATTGAAGCCATGTTGCGTGGGAATAAGCAGCGAGTATTCATGTGGGAGTGTGGGCGTGAATGGGGAGAAGAAGTATACAATATGTGAAGATCCAGGAGCTGCATTCTTCTGGGACGCAGTGCACCCTTCTCAGCAGGGCTGGTTCGCTGTCTACTCTGCATTGGAAGCCAATCTAAAGCAGCTTTAA

mRNA sequence

ATGGCCTCCAAAAAGCTCTTCTTCTACTTCTTCTTCTCTCTCCTCCCAGGTTTCCCTCTTCCCCACTTTCTCTCTCTAAACCCCCCTCCCCCCCTCTCTCTCTCTCTCTATATCATCGGTGTTTTTTTTTTTGTTTTGTTTTGGGTTTTTGTTGCAGAACTATACGGGGTGGTTCAGGTTTCAGCTGCCCACGGCGGCCACCACCGTCTCCGGCGGAGCAGCCTGATCCTCGGAGCAGGCCAGCTCTCGCCCGGTTCGCAGCCCAGAAAGCTGTTTGTGTTTGGGGACTCGTACGTCGACACCGGAAACAACCGGAAACCGGCGGCGAAATCTTGGCAGTACCCCTACGGAATTACCTTCCCCGGAAAACCCACCGGCCGTTTCTCCGATGGCCGTGTTTTGACCGATTACCTAGCTAAGTTTCTGGGAGTGAAGTCTCCGATTCCGTACAAATGGCGGAAAGTAGGGTTCCGGCAGCTGAAATACGGGATGAACTTTGCGTACGGCGGCACCGGCGTGTTCGATACCTTCATTTTGAGCCCAAACATGAGCACTCAGATCGACTTCCTCCGGCAGCTCATCGACGAGTCCACCTTCACCAGCCAAGACTTGCAGTCCTCGGTGGCGCTCATCTCGCTCGCCGGCAACGACTATGGTGTCTATGAAGCCAGAAATGGCTCCACTGAGGGTTGGCAGCCATTTATAACCAAAGTGGTGAACCAGCTGGAGTTGAACCTGAGGCGCATCCATGGGCTGGGGATTCCAAAGATAGTGGTGACGGCTCTGGAGCCACTGGGCTGCCTTCCCAGCAGCACAGTCGCGTCCTCCTTCCAGCAGTGTAATGAAACTCAGAATCTGCTCGTCAACTTCCACAATCTGCTGCTGCAGCAAGCTGTGGCAAAACTCAACAGTGAAGTCAAAGATGACTCTGCTTCTACTTTCATCCTTCTTGATCTCTACTTCTCTTTCATGGCTGCTCTCAACAACAAAGGAGACCATCTTGGGAATGTGAAGTTTGAGAACCCATTGAAGCCATGTTGCGTGGGAATAAGCAGCGAGTATTCATGTGGGAGTGTGGGCGTGAATGGGGAGAAGAAGTATACAATATGTGAAGATCCAGGAGCTGCATTCTTCTGGGACGCAGTGCACCCTTCTCAGCAGGGCTGGTTCGCTGTCTACTCTGCATTGGAAGCCAATCTAAAGCAGCTTTAA

Coding sequence (CDS)

ATGGCCTCCAAAAAGCTCTTCTTCTACTTCTTCTTCTCTCTCCTCCCAGGTTTCCCTCTTCCCCACTTTCTCTCTCTAAACCCCCCTCCCCCCCTCTCTCTCTCTCTCTATATCATCGGTGTTTTTTTTTTTGTTTTGTTTTGGGTTTTTGTTGCAGAACTATACGGGGTGGTTCAGGTTTCAGCTGCCCACGGCGGCCACCACCGTCTCCGGCGGAGCAGCCTGATCCTCGGAGCAGGCCAGCTCTCGCCCGGTTCGCAGCCCAGAAAGCTGTTTGTGTTTGGGGACTCGTACGTCGACACCGGAAACAACCGGAAACCGGCGGCGAAATCTTGGCAGTACCCCTACGGAATTACCTTCCCCGGAAAACCCACCGGCCGTTTCTCCGATGGCCGTGTTTTGACCGATTACCTAGCTAAGTTTCTGGGAGTGAAGTCTCCGATTCCGTACAAATGGCGGAAAGTAGGGTTCCGGCAGCTGAAATACGGGATGAACTTTGCGTACGGCGGCACCGGCGTGTTCGATACCTTCATTTTGAGCCCAAACATGAGCACTCAGATCGACTTCCTCCGGCAGCTCATCGACGAGTCCACCTTCACCAGCCAAGACTTGCAGTCCTCGGTGGCGCTCATCTCGCTCGCCGGCAACGACTATGGTGTCTATGAAGCCAGAAATGGCTCCACTGAGGGTTGGCAGCCATTTATAACCAAAGTGGTGAACCAGCTGGAGTTGAACCTGAGGCGCATCCATGGGCTGGGGATTCCAAAGATAGTGGTGACGGCTCTGGAGCCACTGGGCTGCCTTCCCAGCAGCACAGTCGCGTCCTCCTTCCAGCAGTGTAATGAAACTCAGAATCTGCTCGTCAACTTCCACAATCTGCTGCTGCAGCAAGCTGTGGCAAAACTCAACAGTGAAGTCAAAGATGACTCTGCTTCTACTTTCATCCTTCTTGATCTCTACTTCTCTTTCATGGCTGCTCTCAACAACAAAGGAGACCATCTTGGGAATGTGAAGTTTGAGAACCCATTGAAGCCATGTTGCGTGGGAATAAGCAGCGAGTATTCATGTGGGAGTGTGGGCGTGAATGGGGAGAAGAAGTATACAATATGTGAAGATCCAGGAGCTGCATTCTTCTGGGACGCAGTGCACCCTTCTCAGCAGGGCTGGTTCGCTGTCTACTCTGCATTGGAAGCCAATCTAAAGCAGCTTTAA

Protein sequence

MASKKLFFYFFFSLLPGFPLPHFLSLNPPPPLSLSLYIIGVFFFVLFWVFVAELYGVVQVSAAHGGHHRLRRSSLILGAGQLSPGSQPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQLKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYGVYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQCNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKFENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEANLKQL
Homology
BLAST of Sgr022874 vs. NCBI nr
Match: XP_022144802.1 (GDSL esterase/lipase At5g03610-like [Momordica charantia])

HSP 1 Score: 629.0 bits (1621), Expect = 2.8e-176
Identity = 309/363 (85.12%), Postives = 335/363 (92.29%), Query Frame = 0

Query: 41  VFFFVLFWVFVAELYGVVQVSAAHGGHHRLRRSSLILGAGQLSPGSQPRKLFVFGDSYVD 100
           +FF +L +  + ELYGV++VSAA G  HR+RRSS  LG  +LS GSQP+KLFVFGDSYVD
Sbjct: 7   LFFSLLSFSLLPELYGVIRVSAAAG--HRVRRSSQSLGGVELSAGSQPKKLFVFGDSYVD 66

Query: 101 TGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQL 160
           TGN+RKPAAKSW+YPYGITFPGKPTGRFSDGRVLTDYLAK+LGVKSPIPYKWRKVGFRQL
Sbjct: 67  TGNHRKPAAKSWKYPYGITFPGKPTGRFSDGRVLTDYLAKYLGVKSPIPYKWRKVGFRQL 126

Query: 161 KYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYGV 220
           K GMNFAYGGTGVFDTFILSPNMSTQIDFL+QLIDEST+TSQDLQSSVALISLAGNDY V
Sbjct: 127 KNGMNFAYGGTGVFDTFILSPNMSTQIDFLQQLIDESTYTSQDLQSSVALISLAGNDYAV 186

Query: 221 YEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQC 280
           Y+A NGS +G+Q FITKVVNQLE+NLRRIHGLG+PKIVVTALEPLGCLPSSTVASSFQQC
Sbjct: 187 YQATNGSAQGFQAFITKVVNQLEVNLRRIHGLGVPKIVVTALEPLGCLPSSTVASSFQQC 246

Query: 281 NETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKFE 340
           N T+NLLVNFHNLLLQQ+VAKLNSE+K+ ++STFILLDLY SFMAALNNKGDHLGNVKFE
Sbjct: 247 NATENLLVNFHNLLLQQSVAKLNSELKNGTSSTFILLDLYSSFMAALNNKGDHLGNVKFE 306

Query: 341 NPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEANL 400
           NPLKPCCVGIS+ YSCGSV  NGE KY ICEDP AAFFWD VHPSQQGWFAVYSALEANL
Sbjct: 307 NPLKPCCVGISNAYSCGSVDANGE-KYAICEDPKAAFFWDEVHPSQQGWFAVYSALEANL 366

Query: 401 KQL 404
           KQL
Sbjct: 367 KQL 366

BLAST of Sgr022874 vs. NCBI nr
Match: XP_023539454.1 (GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 628.2 bits (1619), Expect = 4.7e-176
Identity = 308/364 (84.62%), Postives = 330/364 (90.66%), Query Frame = 0

Query: 43  FFVLFWVFVAELYGVVQVSAAHGGH---HRLRRSSLILGAGQLSPGSQPRKLFVFGDSYV 102
           F +L +  + ELYGVV VSA  GGH   HR+RRS   LG G+LS  S PRKLFVFGDSYV
Sbjct: 9   FSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGELS-DSNPRKLFVFGDSYV 68

Query: 103 DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQ 162
           DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAK+L VKSPI YKWRKVG  Q
Sbjct: 69  DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKWRKVGLGQ 128

Query: 163 LKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYG 222
           LKYGMNFAYGGTGVF+TFI+SPNMSTQIDFL+QLI EST+T+QDL  SVALISLAGNDYG
Sbjct: 129 LKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTTQDLHYSVALISLAGNDYG 188

Query: 223 VYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQ 282
           VY+A +GS +GWQPFITKVVNQLE+NLRRIHGLG+PKIVVT+LEPLGCLPSSTVASSFQQ
Sbjct: 189 VYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQ 248

Query: 283 CNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKF 342
           CN T+NLLVNFHNLLLQQ+VAKLNSEVKD SASTFILLDLY SFMAALNNKGD LGNVKF
Sbjct: 249 CNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGDPLGNVKF 308

Query: 343 ENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEAN 402
           ENPLKPCCVGISSEY+CG+VG NGEKKYTICEDPGAAFFWD VHPSQ GWFAVYSAL+AN
Sbjct: 309 ENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSALQAN 368

Query: 403 LKQL 404
           LKQL
Sbjct: 369 LKQL 371

BLAST of Sgr022874 vs. NCBI nr
Match: KAG6596562.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 624.8 bits (1610), Expect = 5.2e-175
Identity = 306/364 (84.07%), Postives = 329/364 (90.38%), Query Frame = 0

Query: 43  FFVLFWVFVAELYGVVQVSAAHGGH---HRLRRSSLILGAGQLSPGSQPRKLFVFGDSYV 102
           F +L +  + ELYGV+ VSA  GGH   HR+RRS   LG G+LS  S PRKLFVFGDSYV
Sbjct: 9   FSLLCFSLLPELYGVIWVSAGGGGHRHRHRVRRSGQSLGGGELS-DSNPRKLFVFGDSYV 68

Query: 103 DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQ 162
           DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAK+L VKSPI YKWRKVG  Q
Sbjct: 69  DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKWRKVGLGQ 128

Query: 163 LKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYG 222
           LKYGMNFAYGGTGVF+TFI+SPNMSTQIDFL+QLI EST+T+QDL  SVALISLAGNDYG
Sbjct: 129 LKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALISLAGNDYG 188

Query: 223 VYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQ 282
           VY+A +GS +GWQPFITKVVNQLE+NLRRIHGLG+PKIVVT+LEPLGCLPSSTVASSFQQ
Sbjct: 189 VYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQ 248

Query: 283 CNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKF 342
           CN T+NLLVNFHNLLLQQ+VAKLNSEVKD SASTFILLDLY SFMAALNNK D LGNVKF
Sbjct: 249 CNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKEDPLGNVKF 308

Query: 343 ENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEAN 402
           ENPLKPCCVGISSEY+CG+VG NGEKKYTICEDPGAAFFWD VHPSQ GWFAVYSAL+AN
Sbjct: 309 ENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSALQAN 368

Query: 403 LKQL 404
           LKQL
Sbjct: 369 LKQL 371

BLAST of Sgr022874 vs. NCBI nr
Match: XP_022926249.1 (GDSL esterase/lipase At5g03610-like [Cucurbita moschata])

HSP 1 Score: 624.8 bits (1610), Expect = 5.2e-175
Identity = 306/364 (84.07%), Postives = 328/364 (90.11%), Query Frame = 0

Query: 43  FFVLFWVFVAELYGVVQVSAAHGGH---HRLRRSSLILGAGQLSPGSQPRKLFVFGDSYV 102
           F +L +  + ELYGVV VS   GGH   HR+RRS   LG G+LS  S PRKLFVFGDSYV
Sbjct: 9   FSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELS-DSNPRKLFVFGDSYV 68

Query: 103 DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQ 162
           DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAK+L VKSPI YKWRKVG  Q
Sbjct: 69  DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKWRKVGLGQ 128

Query: 163 LKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYG 222
           LKYGMNFAYGGTGVF+TFI+SPNMSTQIDFL+QLI EST+T+QDL  SVALISLAGNDYG
Sbjct: 129 LKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALISLAGNDYG 188

Query: 223 VYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQ 282
           VY+A +GS +GWQPFITKVVNQLE+NLRRIHGLG+PKIVVT+LEPLGCLPSSTVASSF Q
Sbjct: 189 VYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFHQ 248

Query: 283 CNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKF 342
           CN T+NLLVNFHNLLLQQ+VAKLNSEVKD SASTFILLDLY SFMAALNNKGD LGNVKF
Sbjct: 249 CNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGDPLGNVKF 308

Query: 343 ENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEAN 402
           ENPLKPCCVGISSEY+CG+VG NGEKKYTICEDPGAAFFWD VHPSQ GWFAVYSAL+AN
Sbjct: 309 ENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSALQAN 368

Query: 403 LKQL 404
           LKQL
Sbjct: 369 LKQL 371

BLAST of Sgr022874 vs. NCBI nr
Match: XP_038905125.1 (GDSL esterase/lipase At5g03610 [Benincasa hispida])

HSP 1 Score: 624.4 bits (1609), Expect = 6.8e-175
Identity = 301/359 (83.84%), Postives = 324/359 (90.25%), Query Frame = 0

Query: 45  VLFWVFVAELYGVVQVSAAHGGHHRLRRSSLILGAGQLSPGSQPRKLFVFGDSYVDTGNN 104
           +L +  + ELYGVV     H   HR RR S  LG G+LS  S PRK+FVFGDSYVDTGNN
Sbjct: 11  LLCFSLLPELYGVVGGGGGHHHRHRERRRSQSLGGGELS-DSNPRKMFVFGDSYVDTGNN 70

Query: 105 RKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQLKYGM 164
           RKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAK+L VKSPIPYKWRKVGF QLKYGM
Sbjct: 71  RKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLKVKSPIPYKWRKVGFGQLKYGM 130

Query: 165 NFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYGVYEAR 224
           NFAYGGTGVFDTF++SPNMSTQIDFL QLI ESTFTS+DLQSSVAL+SLAGNDY VY+A 
Sbjct: 131 NFAYGGTGVFDTFVMSPNMSTQIDFLHQLIGESTFTSRDLQSSVALVSLAGNDYNVYQAT 190

Query: 225 NGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQCNETQ 284
           NGS +GWQPFI KVVNQLE+NLRRIHGLG+PKIVVTALEPLGCLPS+TVASSFQQCN TQ
Sbjct: 191 NGSAQGWQPFIAKVVNQLEVNLRRIHGLGVPKIVVTALEPLGCLPSTTVASSFQQCNATQ 250

Query: 285 NLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKFENPLK 344
           N LVNFHNLLLQQ+VAKLN+EVKD SASTFILLDLY SFMAALNN+G HLGNVKFENP+K
Sbjct: 251 NQLVNFHNLLLQQSVAKLNNEVKDPSASTFILLDLYSSFMAALNNEGAHLGNVKFENPMK 310

Query: 345 PCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEANLKQL 404
           PCCVGIS+EY+CGSVGVNGEKKYTICEDPGAAFFWD VHP+Q GWFAVYSAL+ANLKQL
Sbjct: 311 PCCVGISNEYACGSVGVNGEKKYTICEDPGAAFFWDEVHPTQYGWFAVYSALQANLKQL 368

BLAST of Sgr022874 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 8.9e-109
Identity = 196/366 (53.55%), Postives = 256/366 (69.95%), Query Frame = 0

Query: 38  IIGVFFFVLFWVFVAELYGVVQ-VSAAHGGHHRLRRSSLILGAGQLSPGSQPRKLFVFGD 97
           +I +FF +  ++  + L+G +  V  ++  HH             L P  +P KLFVFGD
Sbjct: 4   LIKLFFCLFIFLCTSLLFGEINGVEGSNQNHH-------------LYP-FRPTKLFVFGD 63

Query: 98  SYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRK-V 157
           SY DTGN +K  + SW++PYGITFPGKP GRFSDGRV TD+LAKF+G+KSPIPY W+   
Sbjct: 64  SYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYA 123

Query: 158 GFRQLKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDE-STFTSQDLQSSVALISLA 217
           G ++L+YGMNFAYGGTGVF+T    PNM+TQID  + ++     +   +L SSVAL+S+A
Sbjct: 124 GKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVA 183

Query: 218 GNDYGVYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVA 277
           GNDY  + A N     +  FI +VV+Q E+NLRRIH LG+ KI V +L+PLGCLP  T  
Sbjct: 184 GNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFV 243

Query: 278 SSFQQCNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHL 337
           +SFQ+CNETQN LVN HN LLQQ VAKLN+E K    STFI+LDLY +F+    NKG + 
Sbjct: 244 TSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQ---STFIILDLYNAFLTVFKNKGSNP 303

Query: 338 GNVKFENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYS 397
           G+ +FE+PLKPCCVG+S EY+CGSV   G KKY +C++P  AFFWD +HP+++GW +VYS
Sbjct: 304 GSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYS 352

Query: 398 ALEANL 401
            L  +L
Sbjct: 364 VLRESL 352

BLAST of Sgr022874 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 5.8e-100
Identity = 183/340 (53.82%), Postives = 232/340 (68.24%), Query Frame = 0

Query: 73  SSLILGAGQLSPGS------QPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTG 132
           SS  LGA    P        +P +LFVFGDSY DTGN RK  + SW+ PYGITFP KP+G
Sbjct: 16  SSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSG 75

Query: 133 RFSDGRVLTDYLAKFLGVKSPIPYKWRK-VGFRQLKYGMNFAYGGTGVFDTFILS-PNMS 192
           RFSDGRV TD+LA++LG+KSPIPY W+   G  +L YGMN+AYGGTGVF T     PNM+
Sbjct: 76  RFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMT 135

Query: 193 TQID-FLRQLIDESTFTSQDLQSSVALISLAGNDYGVYEARNGSTEGWQPFITKVVNQLE 252
           TQID F R L   + ++  DL SS+AL+S+AGNDY  + A          F+ +VV+Q+ 
Sbjct: 136 TQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIA 195

Query: 253 LNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQCNETQNLLVNFHNLLLQQAVAKLN 312
           +N  RIH LG+ KIV+ +++PLGCLPS TV +SFQ+CN T N   N HN LL +A+A+LN
Sbjct: 196 VNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLN 255

Query: 313 SEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKFENPLKPCCVGISSEYSCGSVGVNG 372
           +E K    STF++LD Y +F+    NKG   G  +F NPLKPCCVG++S Y C +V   G
Sbjct: 256 NETK---PSTFVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKG 315

Query: 373 EKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEANLKQL 404
           EKKY ICEDP AAFFWD  HPS++GW +VYS L  +LK +
Sbjct: 316 EKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLKAI 352

BLAST of Sgr022874 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 291.2 bits (744), Expect = 1.8e-77
Identity = 156/332 (46.99%), Postives = 216/332 (65.06%), Query Frame = 0

Query: 77  LGAGQLSPGSQPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTD 136
           +G+ Q S  ++  KLFVFG+SY DTG N KP A SW+ PYGITFPGKP+GR+SDG   TD
Sbjct: 21  VGSNQPSQANKNPKLFVFGNSYADTG-NMKPTALSWKLPYGITFPGKPSGRYSDGLTATD 80

Query: 137 YLAKFLGVKSPIPYKWRKVGFRQLKY--GMNFAYGGTGVFDTFI-LSPNMSTQIDFLRQL 196
           +LAK LG K  +PY WR  G +++K   GMNFA+GG+ VFD+ +  SPN+STQ+ FL  L
Sbjct: 81  FLAKQLGAK--LPYLWRTHGKKKVKLNRGMNFAFGGSEVFDSPVDRSPNISTQVGFLVNL 140

Query: 197 -IDESTFT-SQDLQSSVALISLAGNDYGVYEARNGSTEGWQPFITKVVNQLELNLRRIHG 256
            +    +T   DL SS AL+S +G DY  +  +N +   +  F+  +V  ++ +L  ++G
Sbjct: 141 ALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAAYPAFVEFIVEDIQYSLGIMNG 200

Query: 257 LGIPKIVVTALEPLGCLPSSTVASSFQQCNETQNLLVNFHNLLLQQAVAKLNSEVK-DDS 316
           L    I VT+L PLGCLP  TVASSF+ CNE+ + LV  HN  L++AVAKLN E K    
Sbjct: 201 LKFKNIAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRLHNESLKKAVAKLNKEDKFRTK 260

Query: 317 ASTFILLDLYFSFMAALNNKGDHLGNVKFENPLKPCCVGISSEYSCGSVGVNGEKKYTIC 376
              F+++DL+ +FM  L  K    GN +F++PLKPCC G      C  + + G KKYT+C
Sbjct: 261 GDRFVIVDLHKAFMTILEKK----GNKRFKSPLKPCCEG-----DCARMDMKGAKKYTLC 320

Query: 377 EDPGAAFFWDAVHPSQQGWFAVYSALEANLKQ 403
            DP +AFFWD ++P+Q+GW ++YS L  +L +
Sbjct: 321 NDPKSAFFWDEINPTQEGWRSIYSLLGKSLTE 340

BLAST of Sgr022874 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 287.3 bits (734), Expect = 2.6e-76
Identity = 152/315 (48.25%), Postives = 196/315 (62.22%), Query Frame = 0

Query: 85  GSQPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGV 144
           G +P KLFVFGDSY DTGN       SW++P GITFPG PTGRFSDGRV TDYLAK++GV
Sbjct: 42  GFKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGV 101

Query: 145 KSPIPYKWRKVGFRQL--KYGMNFAYGGTGVFDT-FILSPNMSTQIDFLRQLIDESTFTS 204
           ++PI YKW K G  +L  K GMNFAYGG G F+T F L P  S QID   QL+  + ++ 
Sbjct: 102 RTPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSP 161

Query: 205 QDLQSSVALISLAGNDYGVYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTA 264
            DL SSVA  S+ GNDY  Y+ RNGS EG      KVV Q+ L+++RI  LG+ K++V  
Sbjct: 162 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 221

Query: 265 LEPLGCLPSSTVASSFQQCNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYF 324
             P  CLP   V       N+T   L   HN LL++ + KLN +  +++  +F+ LDLY 
Sbjct: 222 SPPQKCLP-KLVTPKGCDTNDTSTYL---HNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 281

Query: 325 SFMAALNNKGDHLGNVKFENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDA 384
           +F+    NKG   G   F +P K CC      + CG   ++G+K YT+C+DP + FFWD 
Sbjct: 282 AFVTIFKNKGVS-GVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFWDN 341

Query: 385 VHPSQQGWFAVYSAL 397
           VH S QGW +V+S L
Sbjct: 342 VHISDQGWRSVFSLL 350

BLAST of Sgr022874 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 6.6e-72
Identity = 146/324 (45.06%), Postives = 205/324 (63.27%), Query Frame = 0

Query: 77  LGAGQLSPGSQPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTD 136
           +G+ QL    +  KLFVFGDSY DTGN ++   ++W  PYGITFPGKP+GR+ DG + TD
Sbjct: 1   MGSNQLLRPRKVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATD 60

Query: 137 YLAKFLGVKSPIPYKWRKVG-FRQLKYGMNFAYGGTGVFDTFILS--PNMSTQIDFLRQL 196
           +L K LG +S  PY +R  G  + LK GMNFA+GG+ + D+   S  PN++ Q++FL  L
Sbjct: 61  FLEKVLGAES--PYLYRTHGRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL 120

Query: 197 IDESTFTSQDLQSSVALISLAGNDYGVYEARNGSTEGWQPFITKVVNQLELNLRRIHGLG 256
           +           S V+LIS AG DY  Y  +N    G +  + KVV+ L +N+  + GL 
Sbjct: 121 VLAGRVYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLL 180

Query: 257 IPKIVVTALEPLGCLPSSTVASSFQQCNETQNLLVNFHNLLLQQAVAKLNSEVK-DDSAS 316
             KI VT+L+P+GCLPS T ASSF+ CNE+Q+ LV  HN LL++ VAKLN + +      
Sbjct: 181 FKKIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQ 240

Query: 317 TFILLDLYFSFMAALNNKGDHLGNVKFENPLKPCCVGISSEYSCGSVGVNGEKKYTICED 376
            F ++D++ +FM  + NK    G+ +F+NP+K CC G      CG    +G K YT+C+D
Sbjct: 241 HFFIIDIHNAFMTVMKNK----GSKRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDD 300

Query: 377 PGAAFFWDAVHPSQQGWFAVYSAL 397
           P + FFWDAVHP+Q+GW ++YS L
Sbjct: 301 PKSFFFWDAVHPTQEGWRSIYSVL 311

BLAST of Sgr022874 vs. ExPASy TrEMBL
Match: A0A6J1CUR0 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111014394 PE=3 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 1.3e-176
Identity = 309/363 (85.12%), Postives = 335/363 (92.29%), Query Frame = 0

Query: 41  VFFFVLFWVFVAELYGVVQVSAAHGGHHRLRRSSLILGAGQLSPGSQPRKLFVFGDSYVD 100
           +FF +L +  + ELYGV++VSAA G  HR+RRSS  LG  +LS GSQP+KLFVFGDSYVD
Sbjct: 7   LFFSLLSFSLLPELYGVIRVSAAAG--HRVRRSSQSLGGVELSAGSQPKKLFVFGDSYVD 66

Query: 101 TGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQL 160
           TGN+RKPAAKSW+YPYGITFPGKPTGRFSDGRVLTDYLAK+LGVKSPIPYKWRKVGFRQL
Sbjct: 67  TGNHRKPAAKSWKYPYGITFPGKPTGRFSDGRVLTDYLAKYLGVKSPIPYKWRKVGFRQL 126

Query: 161 KYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYGV 220
           K GMNFAYGGTGVFDTFILSPNMSTQIDFL+QLIDEST+TSQDLQSSVALISLAGNDY V
Sbjct: 127 KNGMNFAYGGTGVFDTFILSPNMSTQIDFLQQLIDESTYTSQDLQSSVALISLAGNDYAV 186

Query: 221 YEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQC 280
           Y+A NGS +G+Q FITKVVNQLE+NLRRIHGLG+PKIVVTALEPLGCLPSSTVASSFQQC
Sbjct: 187 YQATNGSAQGFQAFITKVVNQLEVNLRRIHGLGVPKIVVTALEPLGCLPSSTVASSFQQC 246

Query: 281 NETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKFE 340
           N T+NLLVNFHNLLLQQ+VAKLNSE+K+ ++STFILLDLY SFMAALNNKGDHLGNVKFE
Sbjct: 247 NATENLLVNFHNLLLQQSVAKLNSELKNGTSSTFILLDLYSSFMAALNNKGDHLGNVKFE 306

Query: 341 NPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEANL 400
           NPLKPCCVGIS+ YSCGSV  NGE KY ICEDP AAFFWD VHPSQQGWFAVYSALEANL
Sbjct: 307 NPLKPCCVGISNAYSCGSVDANGE-KYAICEDPKAAFFWDEVHPSQQGWFAVYSALEANL 366

Query: 401 KQL 404
           KQL
Sbjct: 367 KQL 366

BLAST of Sgr022874 vs. ExPASy TrEMBL
Match: A0A6J1EEL4 (GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC111433403 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.5e-175
Identity = 306/364 (84.07%), Postives = 328/364 (90.11%), Query Frame = 0

Query: 43  FFVLFWVFVAELYGVVQVSAAHGGH---HRLRRSSLILGAGQLSPGSQPRKLFVFGDSYV 102
           F +L +  + ELYGVV VS   GGH   HR+RRS   LG G+LS  S PRKLFVFGDSYV
Sbjct: 9   FSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELS-DSNPRKLFVFGDSYV 68

Query: 103 DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQ 162
           DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAK+L VKSPI YKWRKVG  Q
Sbjct: 69  DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKWRKVGLGQ 128

Query: 163 LKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYG 222
           LKYGMNFAYGGTGVF+TFI+SPNMSTQIDFL+QLI EST+T+QDL  SVALISLAGNDYG
Sbjct: 129 LKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALISLAGNDYG 188

Query: 223 VYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQ 282
           VY+A +GS +GWQPFITKVVNQLE+NLRRIHGLG+PKIVVT+LEPLGCLPSSTVASSF Q
Sbjct: 189 VYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFHQ 248

Query: 283 CNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKF 342
           CN T+NLLVNFHNLLLQQ+VAKLNSEVKD SASTFILLDLY SFMAALNNKGD LGNVKF
Sbjct: 249 CNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGDPLGNVKF 308

Query: 343 ENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEAN 402
           ENPLKPCCVGISSEY+CG+VG NGEKKYTICEDPGAAFFWD VHPSQ GWFAVYSAL+AN
Sbjct: 309 ENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSALQAN 368

Query: 403 LKQL 404
           LKQL
Sbjct: 369 LKQL 371

BLAST of Sgr022874 vs. ExPASy TrEMBL
Match: A0A6J1J6I3 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111481670 PE=3 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.3e-175
Identity = 302/363 (83.20%), Postives = 328/363 (90.36%), Query Frame = 0

Query: 43  FFVLFWVFVAELYGVVQVSAAHGGH---HRLRRSSLILGAGQLSPGSQPRKLFVFGDSYV 102
           F +L +  + EL GVV+VSA  G H   HR RRSS  LG G+L   S PRKLFVFGDSYV
Sbjct: 9   FSILCFSLLPELSGVVRVSAGRGSHRHRHRERRSSQSLGGGELW-NSNPRKLFVFGDSYV 68

Query: 103 DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQ 162
           DTGNNRKPAAKSWQYPYG+TFPGKPTGRFSDGRVLTDYLAK+L VKSPIPYKWRKVG+ Q
Sbjct: 69  DTGNNRKPAAKSWQYPYGVTFPGKPTGRFSDGRVLTDYLAKYLKVKSPIPYKWRKVGYGQ 128

Query: 163 LKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYG 222
           LKYGMNFAYGGTGVF+TF++SPNMSTQIDF +QLI E TFTSQDLQSSVAL+SLAGNDY 
Sbjct: 129 LKYGMNFAYGGTGVFNTFVMSPNMSTQIDFFQQLIGELTFTSQDLQSSVALVSLAGNDYS 188

Query: 223 VYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQ 282
           VY+A +GS +GWQPFITKVVNQLE+NLRRIHG+G+PKIVVTALEPLGCLPSSTVASSFQQ
Sbjct: 189 VYQATDGSAQGWQPFITKVVNQLEVNLRRIHGVGVPKIVVTALEPLGCLPSSTVASSFQQ 248

Query: 283 CNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKF 342
           CN T+N LVNFHN+LLQQ+VAKLNSEVKD SASTFILLDLY SFMAALNNKGDHLGNVKF
Sbjct: 249 CNATENQLVNFHNMLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGDHLGNVKF 308

Query: 343 ENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEAN 402
           ENPLKPCCVG+SSEYSCGS+G NGEKKYTICEDPGAAFFWD VHP+Q GWFAVYSAL+AN
Sbjct: 309 ENPLKPCCVGVSSEYSCGSMGSNGEKKYTICEDPGAAFFWDEVHPTQHGWFAVYSALQAN 368

BLAST of Sgr022874 vs. ExPASy TrEMBL
Match: A0A6J1L3E4 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111498754 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.3e-174
Identity = 306/364 (84.07%), Postives = 329/364 (90.38%), Query Frame = 0

Query: 43  FFVLFWVFVAELYGVVQVSAAHGGH---HRLRRSSLILGAGQLSPGSQPRKLFVFGDSYV 102
           F +L +  + ELYGVV VSA   GH   HR+RRS   LG G+LS  S PRKLFVFGDSYV
Sbjct: 9   FSLLCFSLLPELYGVVWVSAGGVGHRHRHRVRRSGQRLGGGELS-DSNPRKLFVFGDSYV 68

Query: 103 DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQ 162
           DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAK+L VKSPI YKWRKVG  Q
Sbjct: 69  DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKWRKVGLGQ 128

Query: 163 LKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYG 222
           LKYGMNFAYGGTGVF+TFI+SPNMSTQIDFL+QLI EST+T+QDL  SVALISLAGNDYG
Sbjct: 129 LKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALISLAGNDYG 188

Query: 223 VYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQ 282
           VY+A +GS +GWQPFITKVVNQLE+NLRRIHGLG+PKIVVT+LEPLGCLPSSTVASSFQQ
Sbjct: 189 VYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQ 248

Query: 283 CNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKF 342
           CN T+NLLVNFHNLLLQQ+VAKLNSEVKD SASTFILLDLY SFMAALNNKGD LGNVKF
Sbjct: 249 CNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGDPLGNVKF 308

Query: 343 ENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEAN 402
           ENPLKPCCVGISSEY+CG+VG NGEKKYTICE+PGAAFFWD VHPSQ GWFAVYSAL+AN
Sbjct: 309 ENPLKPCCVGISSEYACGNVGANGEKKYTICENPGAAFFWDEVHPSQNGWFAVYSALQAN 368

Query: 403 LKQL 404
           LKQL
Sbjct: 369 LKQL 371

BLAST of Sgr022874 vs. ExPASy TrEMBL
Match: A0A6J1FAT3 (GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC111442352 PE=3 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 1.6e-174
Identity = 301/363 (82.92%), Postives = 329/363 (90.63%), Query Frame = 0

Query: 43  FFVLFWVFVAELYGVVQVSA---AHGGHHRLRRSSLILGAGQLSPGSQPRKLFVFGDSYV 102
           F +L +  + EL GVV+VSA   +H   HR RRSS  LG G+L   S PRKLFVFGDSYV
Sbjct: 9   FSILCFSLLPELSGVVRVSAGRVSHRHRHRERRSSQSLGGGELW-NSNPRKLFVFGDSYV 68

Query: 103 DTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQ 162
           DTGNNRKPAAKSWQYPYG+TFPGKPTGRFSDGRVLTDYLAK+L VKSPIPYKWRKVG+ Q
Sbjct: 69  DTGNNRKPAAKSWQYPYGVTFPGKPTGRFSDGRVLTDYLAKYLKVKSPIPYKWRKVGYGQ 128

Query: 163 LKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDESTFTSQDLQSSVALISLAGNDYG 222
           LKYGMNFAYGGTGVF+TF++SPNMSTQIDF +QLI ESTFTSQDLQSSVAL+SLAGNDY 
Sbjct: 129 LKYGMNFAYGGTGVFNTFVMSPNMSTQIDFFQQLIGESTFTSQDLQSSVALVSLAGNDYS 188

Query: 223 VYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQ 282
           VY+A +GS +GWQPFITKVVNQLE+NLRRIHG+G+PKIVVTALEPLGCLPSSTVASSFQQ
Sbjct: 189 VYQATDGSAQGWQPFITKVVNQLEVNLRRIHGVGVPKIVVTALEPLGCLPSSTVASSFQQ 248

Query: 283 CNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKF 342
           CN T+N LVNFHN+LLQQ+VAKLNSEVKD SASTFILLDLY SFMAALNNKGDHLGNVKF
Sbjct: 249 CNATENQLVNFHNMLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGDHLGNVKF 308

Query: 343 ENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEAN 402
           ENPLKPCCVG+SSEYSCGS+G NGEKKYTIC+DPGAAFFWD VHP+Q GWFAVYSAL+AN
Sbjct: 309 ENPLKPCCVGVSSEYSCGSMGSNGEKKYTICDDPGAAFFWDEVHPTQHGWFAVYSALQAN 368

BLAST of Sgr022874 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 395.2 bits (1014), Expect = 6.3e-110
Identity = 196/366 (53.55%), Postives = 256/366 (69.95%), Query Frame = 0

Query: 38  IIGVFFFVLFWVFVAELYGVVQ-VSAAHGGHHRLRRSSLILGAGQLSPGSQPRKLFVFGD 97
           +I +FF +  ++  + L+G +  V  ++  HH             L P  +P KLFVFGD
Sbjct: 4   LIKLFFCLFIFLCTSLLFGEINGVEGSNQNHH-------------LYP-FRPTKLFVFGD 63

Query: 98  SYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRK-V 157
           SY DTGN +K  + SW++PYGITFPGKP GRFSDGRV TD+LAKF+G+KSPIPY W+   
Sbjct: 64  SYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYA 123

Query: 158 GFRQLKYGMNFAYGGTGVFDTFILSPNMSTQIDFLRQLIDE-STFTSQDLQSSVALISLA 217
           G ++L+YGMNFAYGGTGVF+T    PNM+TQID  + ++     +   +L SSVAL+S+A
Sbjct: 124 GKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVA 183

Query: 218 GNDYGVYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVA 277
           GNDY  + A N     +  FI +VV+Q E+NLRRIH LG+ KI V +L+PLGCLP  T  
Sbjct: 184 GNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFV 243

Query: 278 SSFQQCNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYFSFMAALNNKGDHL 337
           +SFQ+CNETQN LVN HN LLQQ VAKLN+E K    STFI+LDLY +F+    NKG + 
Sbjct: 244 TSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQ---STFIILDLYNAFLTVFKNKGSNP 303

Query: 338 GNVKFENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYS 397
           G+ +FE+PLKPCCVG+S EY+CGSV   G KKY +C++P  AFFWD +HP+++GW +VYS
Sbjct: 304 GSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYS 352

Query: 398 ALEANL 401
            L  +L
Sbjct: 364 VLRESL 352

BLAST of Sgr022874 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 365.9 bits (938), Expect = 4.1e-101
Identity = 183/340 (53.82%), Postives = 232/340 (68.24%), Query Frame = 0

Query: 73  SSLILGAGQLSPGS------QPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTG 132
           SS  LGA    P        +P +LFVFGDSY DTGN RK  + SW+ PYGITFP KP+G
Sbjct: 16  SSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSG 75

Query: 133 RFSDGRVLTDYLAKFLGVKSPIPYKWRK-VGFRQLKYGMNFAYGGTGVFDTFILS-PNMS 192
           RFSDGRV TD+LA++LG+KSPIPY W+   G  +L YGMN+AYGGTGVF T     PNM+
Sbjct: 76  RFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMT 135

Query: 193 TQID-FLRQLIDESTFTSQDLQSSVALISLAGNDYGVYEARNGSTEGWQPFITKVVNQLE 252
           TQID F R L   + ++  DL SS+AL+S+AGNDY  + A          F+ +VV+Q+ 
Sbjct: 136 TQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIA 195

Query: 253 LNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQCNETQNLLVNFHNLLLQQAVAKLN 312
           +N  RIH LG+ KIV+ +++PLGCLPS TV +SFQ+CN T N   N HN LL +A+A+LN
Sbjct: 196 VNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLN 255

Query: 313 SEVKDDSASTFILLDLYFSFMAALNNKGDHLGNVKFENPLKPCCVGISSEYSCGSVGVNG 372
           +E K    STF++LD Y +F+    NKG   G  +F NPLKPCCVG++S Y C +V   G
Sbjct: 256 NETK---PSTFVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKG 315

Query: 373 EKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEANLKQL 404
           EKKY ICEDP AAFFWD  HPS++GW +VYS L  +LK +
Sbjct: 316 EKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLKAI 352

BLAST of Sgr022874 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.8e-77
Identity = 152/315 (48.25%), Postives = 196/315 (62.22%), Query Frame = 0

Query: 85  GSQPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGV 144
           G +P KLFVFGDSY DTGN       SW++P GITFPG PTGRFSDGRV TDYLAK++GV
Sbjct: 42  GFKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGV 101

Query: 145 KSPIPYKWRKVGFRQL--KYGMNFAYGGTGVFDT-FILSPNMSTQIDFLRQLIDESTFTS 204
           ++PI YKW K G  +L  K GMNFAYGG G F+T F L P  S QID   QL+  + ++ 
Sbjct: 102 RTPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSP 161

Query: 205 QDLQSSVALISLAGNDYGVYEARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTA 264
            DL SSVA  S+ GNDY  Y+ RNGS EG      KVV Q+ L+++RI  LG+ K++V  
Sbjct: 162 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 221

Query: 265 LEPLGCLPSSTVASSFQQCNETQNLLVNFHNLLLQQAVAKLNSEVKDDSASTFILLDLYF 324
             P  CLP   V       N+T   L   HN LL++ + KLN +  +++  +F+ LDLY 
Sbjct: 222 SPPQKCLP-KLVTPKGCDTNDTSTYL---HNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 281

Query: 325 SFMAALNNKGDHLGNVKFENPLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDA 384
           +F+    NKG   G   F +P K CC      + CG   ++G+K YT+C+DP + FFWD 
Sbjct: 282 AFVTIFKNKGVS-GVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFWDN 341

Query: 385 VHPSQQGWFAVYSAL 397
           VH S QGW +V+S L
Sbjct: 342 VHISDQGWRSVFSLL 350

BLAST of Sgr022874 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 272.7 bits (696), Expect = 4.7e-73
Identity = 146/324 (45.06%), Postives = 205/324 (63.27%), Query Frame = 0

Query: 77  LGAGQLSPGSQPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTD 136
           +G+ QL    +  KLFVFGDSY DTGN ++   ++W  PYGITFPGKP+GR+ DG + TD
Sbjct: 1   MGSNQLLRPRKVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATD 60

Query: 137 YLAKFLGVKSPIPYKWRKVG-FRQLKYGMNFAYGGTGVFDTFILS--PNMSTQIDFLRQL 196
           +L K LG +S  PY +R  G  + LK GMNFA+GG+ + D+   S  PN++ Q++FL  L
Sbjct: 61  FLEKVLGAES--PYLYRTHGRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL 120

Query: 197 IDESTFTSQDLQSSVALISLAGNDYGVYEARNGSTEGWQPFITKVVNQLELNLRRIHGLG 256
           +           S V+LIS AG DY  Y  +N    G +  + KVV+ L +N+  + GL 
Sbjct: 121 VLAGRVYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLL 180

Query: 257 IPKIVVTALEPLGCLPSSTVASSFQQCNETQNLLVNFHNLLLQQAVAKLNSEVK-DDSAS 316
             KI VT+L+P+GCLPS T ASSF+ CNE+Q+ LV  HN LL++ VAKLN + +      
Sbjct: 181 FKKIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQ 240

Query: 317 TFILLDLYFSFMAALNNKGDHLGNVKFENPLKPCCVGISSEYSCGSVGVNGEKKYTICED 376
            F ++D++ +FM  + NK    G+ +F+NP+K CC G      CG    +G K YT+C+D
Sbjct: 241 HFFIIDIHNAFMTVMKNK----GSKRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDD 300

Query: 377 PGAAFFWDAVHPSQQGWFAVYSAL 397
           P + FFWDAVHP+Q+GW ++YS L
Sbjct: 301 PKSFFFWDAVHPTQEGWRSIYSVL 311

BLAST of Sgr022874 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 169.9 bits (429), Expect = 4.3e-42
Identity = 105/301 (34.88%), Postives = 149/301 (49.50%), Query Frame = 0

Query: 106 KPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKFLGVKSPIPYKWRKVGFRQLKYGMN 165
           KP A SW+ PYGITFPGKP+GR+SDG   TD+L                           
Sbjct: 2   KPTALSWKLPYGITFPGKPSGRYSDGLTATDFL--------------------------- 61

Query: 166 FAYGGTGVFDTFI-LSPNMSTQIDFLRQL-IDESTFT-SQDLQSSVALISLAGNDYGVYE 225
               G+ VFD+ +  SPN+STQ+ FL  L +    +T   DL SS AL+S +G DY  + 
Sbjct: 62  ----GSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFI 121

Query: 226 ARNGSTEGWQPFITKVVNQLELNLRRIHGLGIPKIVVTALEPLGCLPSSTVASSFQQCNE 285
            +N +   +  F+  +V  +                                     CNE
Sbjct: 122 DQNPNMAAYPAFVEFIVEDI------------------------------------HCNE 181

Query: 286 TQNLLVNFHNLLLQQAVAKLNSEVK-DDSASTFILLDLYFSFMAALNNKGDHLGNVKFEN 345
           + + LV  HN  L++AVAKLN E K       F+++DL+ +FM  L  K    GN +F++
Sbjct: 182 SYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILEKK----GNKRFKS 226

Query: 346 PLKPCCVGISSEYSCGSVGVNGEKKYTICEDPGAAFFWDAVHPSQQGWFAVYSALEANLK 403
           PLKPCC G      C  + + G KKYT+C DP +AFFWD ++P+Q+GW ++YS L  +L 
Sbjct: 242 PLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLLGKSLT 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144802.12.8e-17685.12GDSL esterase/lipase At5g03610-like [Momordica charantia][more]
XP_023539454.14.7e-17684.62GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo][more]
KAG6596562.15.2e-17584.07GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022926249.15.2e-17584.07GDSL esterase/lipase At5g03610-like [Cucurbita moschata][more]
XP_038905125.16.8e-17583.84GDSL esterase/lipase At5g03610 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LZS78.9e-10953.55GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2... [more]
Q9SF945.8e-10053.82GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2... [more]
Q9LZS91.8e-7746.99GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3... [more]
Q8RWJ42.6e-7648.25GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2... [more]
Q9LZS86.6e-7245.06GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1CUR01.3e-17685.12GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC1110143... [more]
A0A6J1EEL42.5e-17584.07GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC11143340... [more]
A0A6J1J6I33.3e-17583.20GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111481670 ... [more]
A0A6J1L3E41.3e-17484.07GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111498754 ... [more]
A0A6J1FAT31.6e-17482.92GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC11144235... [more]
Match NameE-valueIdentityDescription
AT5G03610.16.3e-11053.55GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G09930.14.1e-10153.82GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT2G36325.11.8e-7748.25GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G03600.14.7e-7345.06SGNH hydrolase-type esterase superfamily protein [more]
AT5G03590.14.3e-4234.88GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 91..388
e-value: 1.6E-24
score: 87.6
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 83..402
e-value: 3.3E-56
score: 193.0
NoneNo IPR availablePANTHERPTHR46020OSJNBB0059K02.9 PROTEINcoord: 83..397
NoneNo IPR availablePANTHERPTHR46020:SF17GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 83..397
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 90..401
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 91..396
e-value: 7.97102E-91
score: 274.878

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr022874.1Sgr022874.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds