Sgr022756 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr022756
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionE3 ubiquitin-protein ligase SGR9, amyloplastic
Locationtig00000589: 1486656 .. 1487507 (+)
RNA-Seq ExpressionSgr022756
SyntenySgr022756
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAAGAACCACCCACTGTGATCATGGCTGCTCTCTCCACCCTCTCGCCGCCCCATCTCTCCGATCTCTCCCACTCCATCTTCTCCGACATCCACCACCGCCGCCGCCGCCTCACCCTCATCCTCTCCTCCCCCACCCTCTTCTCCCTCACCCTCCGCCACCTCAATTCCCTCTCCCTCTCCCACAAAACTCTCCTCATTGCTCGCTTCCTTCTCTCCGCCCTCCGCCGCCTCTGCCGCCCCTTTCAACCCCCATCCAAGACCCTCCCGCAGCACCCTTCCACCGCCGCCATCACGCCGCGGGACCTCGACGCCGCCGTCCTCCTCCTCCTCCTCTGCGAAGTCCGTCGACATAATCCGACGGCCCTGGAGACGCCGCTCGCCAAATGGCGTGCGACTCTCTGCAGACTCTACTCCGATTCCATCTTGACGATATCCGGCGTCGCCTCCGTCAATGGGGCGGTTTTGATTCCGTACATAGACACTGTGGCGAGGTGCTGGAAGTTCGTGGGTTTTGTTGGAAGCTGCGGCGGGAAGGCGAGGAGGGAGGTGGCGGCGTCTCCGGTGGCGGTGGTTGAGCTGCCGTCGGTGGAGGTGGGAGGCGGTGGGGCGGCGGCGGCTGAATGTGTGATTTGCAAGGAGGAGATGAGAGAAGGGAGAGACGCGTGTCAATTGCCATGTGAACACTTATTCCATTGGCTCTGTATTCTGCCATGGCTGAGGAAGAGGAACACGTGTCCCTGTTGCAGGTTTCAGCTGCCCACCGATGATGTTTTCGGAGAGATCCAACGGCTCTGGGAGATCCTCCTCAAGGTGGGCTCCACGATCAGTCCATCTGATAGATATTAA

mRNA sequence

ATGGAAGAAGAACCACCCACTGTGATCATGGCTGCTCTCTCCACCCTCTCGCCGCCCCATCTCTCCGATCTCTCCCACTCCATCTTCTCCGACATCCACCACCGCCGCCGCCGCCTCACCCTCATCCTCTCCTCCCCCACCCTCTTCTCCCTCACCCTCCGCCACCTCAATTCCCTCTCCCTCTCCCACAAAACTCTCCTCATTGCTCGCTTCCTTCTCTCCGCCCTCCGCCGCCTCTGCCGCCCCTTTCAACCCCCATCCAAGACCCTCCCGCAGCACCCTTCCACCGCCGCCATCACGCCGCGGGACCTCGACGCCGCCGTCCTCCTCCTCCTCCTCTGCGAAGTCCGTCGACATAATCCGACGGCCCTGGAGACGCCGCTCGCCAAATGGCGTGCGACTCTCTGCAGACTCTACTCCGATTCCATCTTGACGATATCCGGCGTCGCCTCCGTCAATGGGGCGGTTTTGATTCCGTACATAGACACTGTGGCGAGGTGCTGGAAGTTCGTGGGTTTTGTTGGAAGCTGCGGCGGGAAGGCGAGGAGGGAGGTGGCGGCGTCTCCGGTGGCGGTGGTTGAGCTGCCGTCGGTGGAGGTGGGAGGCGGTGGGGCGGCGGCGGCTGAATGTGTGATTTGCAAGGAGGAGATGAGAGAAGGGAGAGACGCGTGTCAATTGCCATGTGAACACTTATTCCATTGGCTCTGTATTCTGCCATGGCTGAGGAAGAGGAACACGTGTCCCTGTTGCAGGTTTCAGCTGCCCACCGATGATGTTTTCGGAGAGATCCAACGGCTCTGGGAGATCCTCCTCAAGGTGGGCTCCACGATCAGTCCATCTGATAGATATTAA

Coding sequence (CDS)

ATGGAAGAAGAACCACCCACTGTGATCATGGCTGCTCTCTCCACCCTCTCGCCGCCCCATCTCTCCGATCTCTCCCACTCCATCTTCTCCGACATCCACCACCGCCGCCGCCGCCTCACCCTCATCCTCTCCTCCCCCACCCTCTTCTCCCTCACCCTCCGCCACCTCAATTCCCTCTCCCTCTCCCACAAAACTCTCCTCATTGCTCGCTTCCTTCTCTCCGCCCTCCGCCGCCTCTGCCGCCCCTTTCAACCCCCATCCAAGACCCTCCCGCAGCACCCTTCCACCGCCGCCATCACGCCGCGGGACCTCGACGCCGCCGTCCTCCTCCTCCTCCTCTGCGAAGTCCGTCGACATAATCCGACGGCCCTGGAGACGCCGCTCGCCAAATGGCGTGCGACTCTCTGCAGACTCTACTCCGATTCCATCTTGACGATATCCGGCGTCGCCTCCGTCAATGGGGCGGTTTTGATTCCGTACATAGACACTGTGGCGAGGTGCTGGAAGTTCGTGGGTTTTGTTGGAAGCTGCGGCGGGAAGGCGAGGAGGGAGGTGGCGGCGTCTCCGGTGGCGGTGGTTGAGCTGCCGTCGGTGGAGGTGGGAGGCGGTGGGGCGGCGGCGGCTGAATGTGTGATTTGCAAGGAGGAGATGAGAGAAGGGAGAGACGCGTGTCAATTGCCATGTGAACACTTATTCCATTGGCTCTGTATTCTGCCATGGCTGAGGAAGAGGAACACGTGTCCCTGTTGCAGGTTTCAGCTGCCCACCGATGATGTTTTCGGAGAGATCCAACGGCTCTGGGAGATCCTCCTCAAGGTGGGCTCCACGATCAGTCCATCTGATAGATATTAA

Protein sequence

MEEEPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSLSLSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRHNPTALETPLAKWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGGKARREVAASPVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPSDRY
Homology
BLAST of Sgr022756 vs. NCBI nr
Match: XP_022145706.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Momordica charantia])

HSP 1 Score: 496.5 bits (1277), Expect = 1.5e-136
Identity = 246/282 (87.23%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 1   MEE-EPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSL 60
           MEE EPPTVIMAALS LSP HLSDLSHSIFSDIHHRRRRLT ILSSPTLFSLTLRHLNSL
Sbjct: 30  MEELEPPTVIMAALSALSPLHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSL 89

Query: 61  SLSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRH 120
           SLSHK+LLIARFLLSALRRLC PFQPP+K+LPQHPSTAAITPRDLDAAVLLLLLCEVR+H
Sbjct: 90  SLSHKSLLIARFLLSALRRLCHPFQPPAKSLPQHPSTAAITPRDLDAAVLLLLLCEVRQH 149

Query: 121 NPTALETPLAKWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGG 180
           NP ALETPL KWRA LCR+YSDSIL+ISG+ S  GAV++PY++TVARCWKFVGFVGSCGG
Sbjct: 150 NPAALETPLPKWRAALCRIYSDSILSISGIISGGGAVMVPYVETVARCWKFVGFVGSCGG 209

Query: 181 KARREVAASPVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILP 240
           K +REVAASPVAVVELPSV      AAA ECVICKEEM EGRDACQLPCEHLFHWLCILP
Sbjct: 210 KGKREVAASPVAVVELPSV----AAAAAVECVICKEEMGEGRDACQLPCEHLFHWLCILP 269

Query: 241 WLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           WLRKRNTCPCCRFQLPTDDVF EIQRLWEILLK+GSTI+ SD
Sbjct: 270 WLRKRNTCPCCRFQLPTDDVFAEIQRLWEILLKLGSTITTSD 307

BLAST of Sgr022756 vs. NCBI nr
Match: XP_016899723.1 (PREDICTED: E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo])

HSP 1 Score: 478.8 bits (1231), Expect = 3.3e-131
Identity = 235/282 (83.33%), Postives = 255/282 (90.43%), Query Frame = 0

Query: 1   MEE-EPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSL 60
           MEE EPP+VIMAAL+TLSPP L+DLSHSIFS++H  RRRLT ILSSPTLFSLTLRHLNSL
Sbjct: 1   MEELEPPSVIMAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSL 60

Query: 61  SLSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRH 120
           SLSHK+LL+ARFLLSALRRL RPFQ PSK LP HPSTAAI+P+DLDAAVLLLLLCEVR+H
Sbjct: 61  SLSHKSLLLARFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQH 120

Query: 121 NPTALETPLAKWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGG 180
           NP AL TP+ KWRATLCR+YS+S+LTISG+A+  G  LIPYIDTV RCWKFVGFVGSCGG
Sbjct: 121 NPAALRTPITKWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSCGG 180

Query: 181 KARREVAASPVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILP 240
           K RREVAASP+AVVELPSV VGGGG    ECVICKEEM EGRDAC+LPC+HLFHWLCILP
Sbjct: 181 KVRREVAASPMAVVELPSVAVGGGG-GTVECVICKEEMGEGRDACKLPCDHLFHWLCILP 240

Query: 241 WLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           WLRKRNTCPCCRFQLPTDDVFGEIQRLWEIL KVGST+  SD
Sbjct: 241 WLRKRNTCPCCRFQLPTDDVFGEIQRLWEILFKVGSTMCTSD 281

BLAST of Sgr022756 vs. NCBI nr
Match: XP_004137953.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis sativus] >KGN58913.1 hypothetical protein Csa_002303 [Cucumis sativus])

HSP 1 Score: 477.6 bits (1228), Expect = 7.3e-131
Identity = 235/282 (83.33%), Postives = 255/282 (90.43%), Query Frame = 0

Query: 1   MEE-EPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSL 60
           MEE EPP+VI AAL+TLSPP L+DLSHSIFS+IH  RRRLT ILSSPTLFSLTLRHLNSL
Sbjct: 1   MEELEPPSVITAALATLSPPRLADLSHSIFSEIHQHRRRLTFILSSPTLFSLTLRHLNSL 60

Query: 61  SLSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRH 120
           SLSHK+LL+ARFLLSALRRL RPFQ PSK LP HPSTAAI+P+DLDAAVLLLLLCEVR+H
Sbjct: 61  SLSHKSLLLARFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQH 120

Query: 121 NPTALETPLAKWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGG 180
           NP AL TP+ KWRATLC++YSDS+LT+SG+A+  G  LIPYIDTV RCWKFVGFVGSCGG
Sbjct: 121 NPAALRTPITKWRATLCKIYSDSLLTVSGLATGGGGALIPYIDTVVRCWKFVGFVGSCGG 180

Query: 181 KARREVAASPVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILP 240
           K RREVAASP+AVVELPSV VGGGG AA ECVICKEEM EGRDAC+LPC+HLFHWLCILP
Sbjct: 181 KVRREVAASPMAVVELPSVAVGGGG-AAVECVICKEEMGEGRDACKLPCDHLFHWLCILP 240

Query: 241 WLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           WLRKRNTCPCCRFQLPTDDV GEIQRLWEIL KVGST+  SD
Sbjct: 241 WLRKRNTCPCCRFQLPTDDVLGEIQRLWEILFKVGSTMCTSD 281

BLAST of Sgr022756 vs. NCBI nr
Match: XP_038905798.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Benincasa hispida])

HSP 1 Score: 474.9 bits (1221), Expect = 4.7e-130
Identity = 234/278 (84.17%), Postives = 249/278 (89.57%), Query Frame = 0

Query: 4   EPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSLSLSH 63
           EPPTVIMAALS LSPP LSDLSHSIFSDIH  RRRLT ILSSPTLFSLTLRHLNSLSLSH
Sbjct: 5   EPPTVIMAALSALSPPSLSDLSHSIFSDIHLHRRRLTFILSSPTLFSLTLRHLNSLSLSH 64

Query: 64  KTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRHNPTA 123
           K+LL+ARFLLS LRRL  PFQ  SK  P HPSTA I+P+DLDAAVLLLLLCEVR+HNP A
Sbjct: 65  KSLLLARFLLSTLRRLSCPFQSQSKLFPHHPSTATISPQDLDAAVLLLLLCEVRQHNPAA 124

Query: 124 LETPLAKWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGGKARR 183
           L TP+ KWRATLCR+YSDS+LTISG+A+  G  LIPYI+TV RCWKFVGFVGSCGGKARR
Sbjct: 125 LRTPITKWRATLCRIYSDSLLTISGIATGGGGALIPYIETVVRCWKFVGFVGSCGGKARR 184

Query: 184 EVAASPVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRK 243
           EVAASPVAVVELPSV VGGGG A  ECVICKEEM EGRDAC+LPC+HLFHWLCILPWLRK
Sbjct: 185 EVAASPVAVVELPSVAVGGGG-ALVECVICKEEMVEGRDACKLPCDHLFHWLCILPWLRK 244

Query: 244 RNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           RNTCPCCRFQLPTDD+FGEIQRLWEILLKVGST+  SD
Sbjct: 245 RNTCPCCRFQLPTDDIFGEIQRLWEILLKVGSTMCTSD 281

BLAST of Sgr022756 vs. NCBI nr
Match: KAA0044054.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo var. makuwa] >TYK25081.1 E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo var. makuwa])

HSP 1 Score: 467.6 bits (1202), Expect = 7.5e-128
Identity = 227/272 (83.46%), Postives = 246/272 (90.44%), Query Frame = 0

Query: 10  MAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSLSLSHKTLLIA 69
           MAAL+TLSPP L+DLSHSIFS++H  RRRLT ILSSPTLFSLTLRHLNSLSLSHK+LL+A
Sbjct: 1   MAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60

Query: 70  RFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRHNPTALETPLA 129
           RFLLSALRRL RPFQ PSK LP HPSTAAI+P+DLDAAVLLLLLCEVR+HNP AL TP+ 
Sbjct: 61  RFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120

Query: 130 KWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGGKARREVAASP 189
           KWRATLCR+YS+S+LTISG+A+  G  LIPYIDTV RCWKFVGFVGSCGGK RREVAASP
Sbjct: 121 KWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSCGGKVRREVAASP 180

Query: 190 VAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRKRNTCPC 249
           +AVVELPSV VGGGG    ECVICKEEM EGRDAC+LPC+HLFHWLCILPWLRKRNTCPC
Sbjct: 181 MAVVELPSVAVGGGG-GTVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPC 240

Query: 250 CRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           CRFQLPTDDVFGEIQRLWEIL KVGST+  SD
Sbjct: 241 CRFQLPTDDVFGEIQRLWEILFKVGSTMCTSD 271

BLAST of Sgr022756 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 4.7e-72
Identity = 154/284 (54.23%), Postives = 187/284 (65.85%), Query Frame = 0

Query: 1   MEEEPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSLS 60
           ME+E  T+IMA+LS LSP HL++L+HSI S  HH RRRL  +LSSPTLFSLTLRHL SLS
Sbjct: 1   MEDENTTIIMASLSALSPSHLTNLTHSILSISHHHRRRLGAVLSSPTLFSLTLRHLLSLS 60

Query: 61  LSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITP--RDLDAAVLLLLLCEVRR 120
           L  KT LIA  LLS L         P     +H S+ A+T   RDLDA VLLL LCE  +
Sbjct: 61  LPDKTHLIANHLLSLLH--------PLLIHRKHHSSYAVTMKLRDLDAVVLLLFLCETHQ 120

Query: 121 HNPTALETPLAKWRATLCRLYSDSILT-ISGVASVNGAVLIPYIDTVARCWKFVGFVGSC 180
            +P  LE     WR  L   YS+++L+  SG+ + +  +L+PYI+T+ RC +FV  +G  
Sbjct: 121 LHPDVLEASADNWREILGNTYSNNMLSNNSGLWTCDAGILMPYIETLVRCKRFVDIMGGY 180

Query: 181 GGKARR------EVAASPVAVVELPSVE---VGGGGAAAAECVICKEEMREGRDACQLPC 240
               RR      +V A+  AVV L +VE   V    A   ECVICKEEM EGRD C++PC
Sbjct: 181 NHLRRRDQKEGYQVPAARAAVVALRAVEVFNVAASNAGEVECVICKEEMSEGRDVCEMPC 240

Query: 241 EHLFHWLCILPWLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLK 273
           +H FHW CILPWL K+NTCP CRFQLPTDDVF EIQRLWEIL+K
Sbjct: 241 QHFFHWKCILPWLSKKNTCPFCRFQLPTDDVFSEIQRLWEILVK 276

BLAST of Sgr022756 vs. ExPASy Swiss-Prot
Match: Q6GPV5 (E3 ubiquitin-protein ligase RNF181 OS=Xenopus laevis OX=8355 GN=rnf181 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 7.6e-14
Identity = 40/72 (55.56%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 187 ASPVAVVELPSVEVGGGGA-AAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRKRN 246
           AS   V  LP V V    A AA +C +C  E  EG    QLPCEHLFH  CILPWL K N
Sbjct: 55  ASKKVVESLPKVTVTPEQADAALKCPVCLLEFEEGETVRQLPCEHLFHSACILPWLGKTN 114

Query: 247 TCPCCRFQLPTD 258
           +CP CR +LPTD
Sbjct: 115 SCPLCRHELPTD 126

BLAST of Sgr022756 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.3e-13
Identity = 34/74 (45.95%), Postives = 48/74 (64.86%), Query Frame = 0

Query: 187 ASPVAVVELPSVEVGGG--GAAAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRKR 246
           AS  A+  LP+V+V      +   +C +C +E  +G D  Q+PC+H+FH  C+LPWL   
Sbjct: 191 ASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELH 250

Query: 247 NTCPCCRFQLPTDD 259
           N+CP CRF+LPTDD
Sbjct: 251 NSCPVCRFELPTDD 264

BLAST of Sgr022756 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.2e-13
Identity = 44/107 (41.12%), Postives = 55/107 (51.40%), Query Frame = 0

Query: 175 GSCGGKARREVAASPVAVVELPSVEVGGG-GAAAAECVICKEEMREGRDACQLPCEHLFH 234
           G  GG  R    A+  A+  L + EV    G     C +CK+ M  G    +LPC H +H
Sbjct: 221 GGGGGGRRGAPPAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHCYH 280

Query: 235 WLCILPWLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPS 281
             CI+PWL  RN+CP CRFQL TDD   E +R      K  ST+S S
Sbjct: 281 GDCIVPWLGTRNSCPVCRFQLETDDAEYEEER-----KKRTSTVSDS 322

BLAST of Sgr022756 vs. ExPASy Swiss-Prot
Match: Q5M974 (E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.2e-13
Identity = 39/72 (54.17%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 187 ASPVAVVELPSVEVGGGGA-AAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRKRN 246
           A+   V  LP V V    A AA +C +C  E  EG    QLPCEHLFH  CILPWL K N
Sbjct: 55  AAKKVVESLPKVTVTPEQADAALKCPVCLLEFEEGETVRQLPCEHLFHSSCILPWLGKTN 114

Query: 247 TCPCCRFQLPTD 258
           +CP CR +LPTD
Sbjct: 115 SCPLCRHELPTD 126

BLAST of Sgr022756 vs. ExPASy TrEMBL
Match: A0A6J1CX45 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Momordica charantia OX=3673 GN=LOC111015097 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 7.3e-137
Identity = 246/282 (87.23%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 1   MEE-EPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSL 60
           MEE EPPTVIMAALS LSP HLSDLSHSIFSDIHHRRRRLT ILSSPTLFSLTLRHLNSL
Sbjct: 30  MEELEPPTVIMAALSALSPLHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSL 89

Query: 61  SLSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRH 120
           SLSHK+LLIARFLLSALRRLC PFQPP+K+LPQHPSTAAITPRDLDAAVLLLLLCEVR+H
Sbjct: 90  SLSHKSLLIARFLLSALRRLCHPFQPPAKSLPQHPSTAAITPRDLDAAVLLLLLCEVRQH 149

Query: 121 NPTALETPLAKWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGG 180
           NP ALETPL KWRA LCR+YSDSIL+ISG+ S  GAV++PY++TVARCWKFVGFVGSCGG
Sbjct: 150 NPAALETPLPKWRAALCRIYSDSILSISGIISGGGAVMVPYVETVARCWKFVGFVGSCGG 209

Query: 181 KARREVAASPVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILP 240
           K +REVAASPVAVVELPSV      AAA ECVICKEEM EGRDACQLPCEHLFHWLCILP
Sbjct: 210 KGKREVAASPVAVVELPSV----AAAAAVECVICKEEMGEGRDACQLPCEHLFHWLCILP 269

Query: 241 WLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           WLRKRNTCPCCRFQLPTDDVF EIQRLWEILLK+GSTI+ SD
Sbjct: 270 WLRKRNTCPCCRFQLPTDDVFAEIQRLWEILLKLGSTITTSD 307

BLAST of Sgr022756 vs. ExPASy TrEMBL
Match: A0A1S4DUS9 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo OX=3656 GN=LOC103486430 PE=4 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.6e-131
Identity = 235/282 (83.33%), Postives = 255/282 (90.43%), Query Frame = 0

Query: 1   MEE-EPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSL 60
           MEE EPP+VIMAAL+TLSPP L+DLSHSIFS++H  RRRLT ILSSPTLFSLTLRHLNSL
Sbjct: 1   MEELEPPSVIMAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSL 60

Query: 61  SLSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRH 120
           SLSHK+LL+ARFLLSALRRL RPFQ PSK LP HPSTAAI+P+DLDAAVLLLLLCEVR+H
Sbjct: 61  SLSHKSLLLARFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQH 120

Query: 121 NPTALETPLAKWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGG 180
           NP AL TP+ KWRATLCR+YS+S+LTISG+A+  G  LIPYIDTV RCWKFVGFVGSCGG
Sbjct: 121 NPAALRTPITKWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSCGG 180

Query: 181 KARREVAASPVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILP 240
           K RREVAASP+AVVELPSV VGGGG    ECVICKEEM EGRDAC+LPC+HLFHWLCILP
Sbjct: 181 KVRREVAASPMAVVELPSVAVGGGG-GTVECVICKEEMGEGRDACKLPCDHLFHWLCILP 240

Query: 241 WLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           WLRKRNTCPCCRFQLPTDDVFGEIQRLWEIL KVGST+  SD
Sbjct: 241 WLRKRNTCPCCRFQLPTDDVFGEIQRLWEILFKVGSTMCTSD 281

BLAST of Sgr022756 vs. ExPASy TrEMBL
Match: A0A0A0LAQ3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G736610 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.5e-131
Identity = 235/282 (83.33%), Postives = 255/282 (90.43%), Query Frame = 0

Query: 1   MEE-EPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSL 60
           MEE EPP+VI AAL+TLSPP L+DLSHSIFS+IH  RRRLT ILSSPTLFSLTLRHLNSL
Sbjct: 1   MEELEPPSVITAALATLSPPRLADLSHSIFSEIHQHRRRLTFILSSPTLFSLTLRHLNSL 60

Query: 61  SLSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRH 120
           SLSHK+LL+ARFLLSALRRL RPFQ PSK LP HPSTAAI+P+DLDAAVLLLLLCEVR+H
Sbjct: 61  SLSHKSLLLARFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQH 120

Query: 121 NPTALETPLAKWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGG 180
           NP AL TP+ KWRATLC++YSDS+LT+SG+A+  G  LIPYIDTV RCWKFVGFVGSCGG
Sbjct: 121 NPAALRTPITKWRATLCKIYSDSLLTVSGLATGGGGALIPYIDTVVRCWKFVGFVGSCGG 180

Query: 181 KARREVAASPVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILP 240
           K RREVAASP+AVVELPSV VGGGG AA ECVICKEEM EGRDAC+LPC+HLFHWLCILP
Sbjct: 181 KVRREVAASPMAVVELPSVAVGGGG-AAVECVICKEEMGEGRDACKLPCDHLFHWLCILP 240

Query: 241 WLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           WLRKRNTCPCCRFQLPTDDV GEIQRLWEIL KVGST+  SD
Sbjct: 241 WLRKRNTCPCCRFQLPTDDVLGEIQRLWEILFKVGSTMCTSD 281

BLAST of Sgr022756 vs. ExPASy TrEMBL
Match: A0A5A7TLA8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002040 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.6e-128
Identity = 227/272 (83.46%), Postives = 246/272 (90.44%), Query Frame = 0

Query: 10  MAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSLSLSHKTLLIA 69
           MAAL+TLSPP L+DLSHSIFS++H  RRRLT ILSSPTLFSLTLRHLNSLSLSHK+LL+A
Sbjct: 1   MAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60

Query: 70  RFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRHNPTALETPLA 129
           RFLLSALRRL RPFQ PSK LP HPSTAAI+P+DLDAAVLLLLLCEVR+HNP AL TP+ 
Sbjct: 61  RFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120

Query: 130 KWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGSCGGKARREVAASP 189
           KWRATLCR+YS+S+LTISG+A+  G  LIPYIDTV RCWKFVGFVGSCGGK RREVAASP
Sbjct: 121 KWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSCGGKVRREVAASP 180

Query: 190 VAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRKRNTCPC 249
           +AVVELPSV VGGGG    ECVICKEEM EGRDAC+LPC+HLFHWLCILPWLRKRNTCPC
Sbjct: 181 MAVVELPSVAVGGGG-GTVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPC 240

Query: 250 CRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           CRFQLPTDDVFGEIQRLWEIL KVGST+  SD
Sbjct: 241 CRFQLPTDDVFGEIQRLWEILFKVGSTMCTSD 271

BLAST of Sgr022756 vs. ExPASy TrEMBL
Match: A0A6J1KTZ6 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucurbita maxima OX=3661 GN=LOC111498644 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 9.9e-126
Identity = 231/283 (81.63%), Postives = 250/283 (88.34%), Query Frame = 0

Query: 1   MEE-EPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSL 60
           MEE +P TVIMAALSTLSPPHLSDLSHSIFSD+H  RRRLT ILSSPTLFS TLRHLNSL
Sbjct: 1   MEELKPSTVIMAALSTLSPPHLSDLSHSIFSDVHTSRRRLTFILSSPTLFSFTLRHLNSL 60

Query: 61  SLSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITPRDLDAAVLLLLLCEVRRH 120
           SLSHK+LL+ARFLLSALRRL RPFQ PSK+ P HPSTA +TPRDLDAAVLLLLLC+VR+H
Sbjct: 61  SLSHKSLLVARFLLSALRRLSRPFQTPSKSFPHHPSTAFVTPRDLDAAVLLLLLCDVRQH 120

Query: 121 NPTALETPLAKWRATLCRLYSDSILTISGVASVNGAVLIPYIDTVARCWKFVGFVGS-CG 180
           NP ALETP+  WR+TLCR+YSDS+LTISG+A+     +IPYI+TV RCWKFVGFV S  G
Sbjct: 121 NPAALETPITHWRSTLCRIYSDSLLTISGIATGGSGAIIPYIETVGRCWKFVGFVVSRNG 180

Query: 181 GKARREVAASPVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCIL 240
           GKARREVAASPVAVVELPSV V  GG  A ECVICKEEM EGRDACQLPCEHLFHWLCIL
Sbjct: 181 GKARREVAASPVAVVELPSVAV--GGCMAVECVICKEEMNEGRDACQLPCEHLFHWLCIL 240

Query: 241 PWLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPSD 282
           PWLRKRNTCPCCRFQLPTDD+FGEIQRLW+IL+KVG   SPSD
Sbjct: 241 PWLRKRNTCPCCRFQLPTDDIFGEIQRLWDILVKVG---SPSD 278

BLAST of Sgr022756 vs. TAIR 10
Match: AT5G02750.1 (RING/U-box superfamily protein )

HSP 1 Score: 272.7 bits (696), Expect = 3.3e-73
Identity = 154/284 (54.23%), Postives = 187/284 (65.85%), Query Frame = 0

Query: 1   MEEEPPTVIMAALSTLSPPHLSDLSHSIFSDIHHRRRRLTLILSSPTLFSLTLRHLNSLS 60
           ME+E  T+IMA+LS LSP HL++L+HSI S  HH RRRL  +LSSPTLFSLTLRHL SLS
Sbjct: 1   MEDENTTIIMASLSALSPSHLTNLTHSILSISHHHRRRLGAVLSSPTLFSLTLRHLLSLS 60

Query: 61  LSHKTLLIARFLLSALRRLCRPFQPPSKTLPQHPSTAAITP--RDLDAAVLLLLLCEVRR 120
           L  KT LIA  LLS L         P     +H S+ A+T   RDLDA VLLL LCE  +
Sbjct: 61  LPDKTHLIANHLLSLLH--------PLLIHRKHHSSYAVTMKLRDLDAVVLLLFLCETHQ 120

Query: 121 HNPTALETPLAKWRATLCRLYSDSILT-ISGVASVNGAVLIPYIDTVARCWKFVGFVGSC 180
            +P  LE     WR  L   YS+++L+  SG+ + +  +L+PYI+T+ RC +FV  +G  
Sbjct: 121 LHPDVLEASADNWREILGNTYSNNMLSNNSGLWTCDAGILMPYIETLVRCKRFVDIMGGY 180

Query: 181 GGKARR------EVAASPVAVVELPSVE---VGGGGAAAAECVICKEEMREGRDACQLPC 240
               RR      +V A+  AVV L +VE   V    A   ECVICKEEM EGRD C++PC
Sbjct: 181 NHLRRRDQKEGYQVPAARAAVVALRAVEVFNVAASNAGEVECVICKEEMSEGRDVCEMPC 240

Query: 241 EHLFHWLCILPWLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLK 273
           +H FHW CILPWL K+NTCP CRFQLPTDDVF EIQRLWEIL+K
Sbjct: 241 QHFFHWKCILPWLSKKNTCPFCRFQLPTDDVFSEIQRLWEILVK 276

BLAST of Sgr022756 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.8e-15
Identity = 35/70 (50.00%), Postives = 46/70 (65.71%), Query Frame = 0

Query: 189 PVAVVELPSVEVGGGGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRKRNTCP 248
           PV ++E  + + GG       C +CK+EM  G  A QLPC H +H  CI+PWL+ RNTCP
Sbjct: 293 PVVLLEGENDDDGG-----LVCAVCKDEMNIGNKAVQLPCNHKYHSECIVPWLKVRNTCP 352

Query: 249 CCRFQLPTDD 259
            CR++LPTDD
Sbjct: 353 VCRYELPTDD 357

BLAST of Sgr022756 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 9.2e-15
Identity = 34/74 (45.95%), Postives = 48/74 (64.86%), Query Frame = 0

Query: 187 ASPVAVVELPSVEVGGG--GAAAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRKR 246
           AS  A+  LP+V+V      +   +C +C +E  +G D  Q+PC+H+FH  C+LPWL   
Sbjct: 191 ASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELH 250

Query: 247 NTCPCCRFQLPTDD 259
           N+CP CRF+LPTDD
Sbjct: 251 NSCPVCRFELPTDD 264

BLAST of Sgr022756 vs. TAIR 10
Match: AT5G64920.1 (COP1-interacting protein 8 )

HSP 1 Score: 77.8 bits (190), Expect = 1.6e-14
Identity = 44/107 (41.12%), Postives = 55/107 (51.40%), Query Frame = 0

Query: 175 GSCGGKARREVAASPVAVVELPSVEVGGG-GAAAAECVICKEEMREGRDACQLPCEHLFH 234
           G  GG  R    A+  A+  L + EV    G     C +CK+ M  G    +LPC H +H
Sbjct: 221 GGGGGGRRGAPPAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHCYH 280

Query: 235 WLCILPWLRKRNTCPCCRFQLPTDDVFGEIQRLWEILLKVGSTISPS 281
             CI+PWL  RN+CP CRFQL TDD   E +R      K  ST+S S
Sbjct: 281 GDCIVPWLGTRNSCPVCRFQLETDDAEYEEER-----KKRTSTVSDS 322

BLAST of Sgr022756 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-14
Identity = 41/92 (44.57%), Postives = 53/92 (57.61%), Query Frame = 0

Query: 187 ASPVAVVELPSVEVGG-GGAAAAECVICKEEMREGRDACQLPCEHLFHWLCILPWLRKRN 246
           AS  A+  LP VE+      A A C +C E    G +  ++PC+H+FH  CI+PWL  RN
Sbjct: 191 ASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRN 250

Query: 247 TCPCCRFQLPTDDVFGEIQRLWEILLKVGSTI 278
           +CP CRF+LP+D     IQR  E    VG TI
Sbjct: 251 SCPVCRFELPSD----PIQRSNEEEHAVGMTI 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145706.11.5e-13687.23E3 ubiquitin-protein ligase SGR9, amyloplastic [Momordica charantia][more]
XP_016899723.13.3e-13183.33PREDICTED: E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo][more]
XP_004137953.17.3e-13183.33E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis sativus] >KGN58913.1 hyp... [more]
XP_038905798.14.7e-13084.17E3 ubiquitin-protein ligase SGR9, amyloplastic [Benincasa hispida][more]
KAA0044054.17.5e-12883.46E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo var. makuwa] >TYK25... [more]
Match NameE-valueIdentityDescription
Q8GXF84.7e-7254.23E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G... [more]
Q6GPV57.6e-1455.56E3 ubiquitin-protein ligase RNF181 OS=Xenopus laevis OX=8355 GN=rnf181 PE=2 SV=1[more]
Q8LPN71.3e-1345.95E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q9SPL22.2e-1341.12E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV... [more]
Q5M9742.2e-1354.17E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CX457.3e-13787.23E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Momordica charantia OX=3673 GN... [more]
A0A1S4DUS91.6e-13183.33E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0LAQ33.5e-13183.33RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G736610 P... [more]
A0A5A7TLA83.6e-12883.46E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1KTZ69.9e-12681.63E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G02750.13.3e-7354.23RING/U-box superfamily protein [more]
AT5G08139.11.8e-1550.00RING/U-box superfamily protein [more]
AT3G19950.19.2e-1545.95RING/U-box superfamily protein [more]
AT5G64920.11.6e-1441.12COP1-interacting protein 8 [more]
AT3G46620.12.0e-1444.57zinc finger (C3HC4-type RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 210..250
e-value: 7.8E-6
score: 35.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 209..251
e-value: 7.1E-11
score: 42.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 210..251
score: 12.1057
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 179..257
e-value: 9.0E-18
score: 65.7
NoneNo IPR availablePANTHERPTHR15710:SF67E3 UBIQUITIN-PROTEIN LIGASE SGR9, AMYLOPLASTICcoord: 3..275
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 3..275
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 203..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr022756.1Sgr022756.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009630 gravitropism
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0009501 amyloplast
molecular_function GO:0061630 ubiquitin protein ligase activity