Sgr022730 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr022730 vs. NCBI nr
Match: KAG6596407.1 (E3 ubiquitin-protein ligase PRT6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3642.4 bits (9444), Expect = 0.0e+00
Identity = 1866/2193 (85.09%), Postives = 1972/2193 (89.92%), Query Frame = 0

Query: 164  FCFELVECCRELDEGHRAWAAHKKEAAWRLRRLELQLESEKASRIRQKMEEVEGKVKALR 223
            F  ELVECCRELDEGHRAWA+HKKE AWRLRR ELQLESEKASRIRQKMEEVEGKVKA+R
Sbjct: 89   FASELVECCRELDEGHRAWASHKKETAWRLRRQELQLESEKASRIRQKMEEVEGKVKAVR 148

Query: 224  EEEKIALERIEAEYKEQLAGLRKDAEAKEQKLAEQWAAKHLSLTKFLEHMGCRTRNSESS 283
            EEEKIALERIEAEYKEQLAGLRKDAEAKEQKLAEQWAAKHLSLTKFLEHMGCRT+ SESS
Sbjct: 149  EEEKIALERIEAEYKEQLAGLRKDAEAKEQKLAEQWAAKHLSLTKFLEHMGCRTKISESS 208

Query: 284  GSTTS------RLRRCFCCADWLVPHWRSRRMDDMDIGSPSESVPLKPRDRIVRRLAVLG 343
            G   +           FC  DWLVP+WR  RMDDMD  SPSES PLKPRDRIV RLAVLG
Sbjct: 209  GRVETWGEQPLGYGAVFCFGDWLVPYWRYWRMDDMDTCSPSESAPLKPRDRIVLRLAVLG 268

Query: 344  VPEDLLDQLQPGLVSFVKDNKFLITELVSAILPTDVEVAEVIRDAKAGSKKSVTGPTMKN 403
            VP++LLDQLQPGLVSFVKDNK+LI +LVS ILPTDVEV + IR+AKAGSKKSVTGPTMKN
Sbjct: 269  VPDELLDQLQPGLVSFVKDNKYLIPQLVSVILPTDVEVVKAIREAKAGSKKSVTGPTMKN 328

Query: 404  QFRESMMWLQWLMFGGEPTFALKNLSKMSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAI 463
            QFRESM WLQWLMF GEP  ALKNLSKMSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAI
Sbjct: 329  QFRESMTWLQWLMFEGEPASALKNLSKMSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAI 388

Query: 464  CVPCFQSGNHKDHDYSIIYTGGGCCDCGDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVG 523
            CVPCFQSGNHKDHDYSII+TGGGCCDCGDVTAWKREGFCSKHKGAEQIQPLPEEFVKSV 
Sbjct: 389  CVPCFQSGNHKDHDYSIIHTGGGCCDCGDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVE 448

Query: 524  PILDALFTSWKNKLLSAEDISVEDPKLSDRVTEHKKIANELTFAVVEMLLDFCKYSESLL 583
            PILD LFT WKNKL+SAE ISVED KLSDRVTEHKK+ANELTFAVVEMLLDFCKYSESLL
Sbjct: 449  PILDVLFTCWKNKLVSAEYISVEDCKLSDRVTEHKKVANELTFAVVEMLLDFCKYSESLL 508

Query: 584  SFVSKRVISSAGFLDILVRLERLLTDIVVKKVHELLLKLLGEPIFKYEFAKVFLNYYPTV 643
            SFVSKRVISSAG LD+LV LERLLT+ VVKKVHELLLKLLGEP+FKYEFAKVFLNYYP V
Sbjct: 509  SFVSKRVISSAGLLDVLVGLERLLTESVVKKVHELLLKLLGEPVFKYEFAKVFLNYYPNV 568

Query: 644  ISEAIEEGSDHAFKKYPLLPTFSVQIFTVPTLTPRLVEEMNLLSILLGCLEDIFISCVSE 703
            ISEAIE+ SDH  KKYPLLPTFSVQIFTVPTL PRLVEEMNLLSILLGCLEDIFISC S+
Sbjct: 569  ISEAIEDNSDHVLKKYPLLPTFSVQIFTVPTLAPRLVEEMNLLSILLGCLEDIFISCSSK 628

Query: 704  DGRLQVIKWSNLYETTIRVVEDIRFVMSHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQ 763
            +GRLQV+KWSNL ETTIRV ED+RFVMSHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQ
Sbjct: 629  NGRLQVLKWSNLCETTIRVAEDVRFVMSHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQ 688

Query: 764  KRETGLHIEEENENVHLPFGLDHSVANIHSLLVNEAFSAA---TGSTEETADAMSFQTYK 823
            KRETG+HIEEENEN+HLPFGLDHSVANIHSLLVNEAFSAA   + S+E+TADAMSFQTYK
Sbjct: 689  KRETGIHIEEENENIHLPFGLDHSVANIHSLLVNEAFSAASSSSSSSEDTADAMSFQTYK 748

Query: 824  QNPEDIDSIRHAKVGRLSQESAACNVLGRSSTSTSASRIDEVCSDAISSTIMWLTYECLK 883
             NP+DIDS+R+AKVGRLSQESAAC++LG+S+TSTSAS++DEV  D IS TIMWLTYECLK
Sbjct: 749  PNPDDIDSVRYAKVGRLSQESAACSLLGKSTTSTSASKVDEVRLDTISPTIMWLTYECLK 808

Query: 884  VIDSWLGTENTSGGIPDMFAENTSMTSSCKLYSLRKTSALESKKMSSKVEKGKFVFEKLA 943
             IDSWLGTEN SGGI +MF E   MT SC  YS RK SALE KKMS K+ KGKF+ EKLA
Sbjct: 809  TIDSWLGTENASGGISNMFDEYIRMTPSCIFYSSRKASALEPKKMSPKMGKGKFILEKLA 868

Query: 944  RKSKNYNRQYSSRMYSGLQMSIDHQHGITLGEDNQLMDVTNDTVTVEDYAMENDALHFLS 1003
            R+SK++N QYSSRMYSGL MSID +HGI+ GEDNQLMD+TNDTVTVEDYAME DAL +LS
Sbjct: 869  RRSKDHNSQYSSRMYSGLPMSIDIEHGISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLS 928

Query: 1004 LSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKALRSCFSESVVPSSTGASSSNLSLEYVD 1063
            +S+WPNIV+DVSSQDISIHIPLHRLLSLLLQKALRSCFSESVV ++TGASSSNLS E VD
Sbjct: 929  MSSWPNIVFDVSSQDISIHIPLHRLLSLLLQKALRSCFSESVVTNATGASSSNLSSESVD 988

Query: 1064 FFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNTGMWRRNGDAALLSCELYRSIRWSEQC 1123
            FFKSVLTDCHPYGFS+F+MEHPLRIKVFCAEVN G WRRNGDAALLSCELYRS+RWSEQC
Sbjct: 989  FFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEVNAGAWRRNGDAALLSCELYRSVRWSEQC 1048

Query: 1124 LELDLFLLQCCAAMAPPDLYVSRILERFRLSDYLSLNVERPSEYEPILVQEMLTLIIQVV 1183
            LELDLFLLQCCAAMAPPD YVSRILERF+LSDYLSLNVE PSEYEPILV+EMLTLIIQVV
Sbjct: 1049 LELDLFLLQCCAAMAPPDFYVSRILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVV 1108

Query: 1184 NERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPRDLSKCHQLQEILDTIAVYSNPS 1243
            NERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPRDLSKC QLQEILDTIAVYSNPS
Sbjct: 1109 NERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPS 1168

Query: 1244 GFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFG 1303
            GFNQGMYSL WKYWKELDLYHPRWSLRDLQIAEERYLRSCGVSALTSQLPKWTKIYPPF 
Sbjct: 1169 GFNQGMYSLHWKYWKELDLYHPRWSLRDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFR 1228

Query: 1304 GLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAPDSVLLSALHLLALALDICFQQKELSD 1363
            GLARIATCK VLQIVRAVLFYSVFTEKSTKSRAPDSVLL+ALHLLALALDICF QKE S+
Sbjct: 1229 GLARIATCKTVLQIVRAVLFYSVFTEKSTKSRAPDSVLLTALHLLALALDICFMQKESSN 1288

Query: 1364 QSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLLSLLILLMKMHKKEGRENLLEAGSCNLS 1423
            QS  + DSIPLLLFADEE+DEGLT GFGRQSLLSLLILLMKMHK+EGRENLL+AGSCNLS
Sbjct: 1289 QSLYARDSIPLLLFADEEIDEGLTCGFGRQSLLSLLILLMKMHKEEGRENLLQAGSCNLS 1348

Query: 1424 SLVECLLKKFSEIDSRCMGKVQQLAPEIFGYLSQSVPSSDTNSLSGTSDSEKRKAKARER 1483
            SLV  LLKKFSEIDS CMGK+QQLAPEI GY +QSVPSS+TNS SGTSDSEKRKAKARER
Sbjct: 1349 SLVGSLLKKFSEIDSCCMGKLQQLAPEILGYRTQSVPSSNTNSPSGTSDSEKRKAKARER 1408

Query: 1484 QAAILEKMRAEQSKFLASFDSSVND-DTEFWQESEKPNVSDSAEQSETVCSLCHDSSSSV 1543
            QAAILEKMRAEQSKFLAS D+S+++ DTE  Q+SEKPNVSDSAEQSETVCSLCHD SSSV
Sbjct: 1409 QAAILEKMRAEQSKFLASVDASMDEYDTEIGQQSEKPNVSDSAEQSETVCSLCHDFSSSV 1468

Query: 1544 PISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHASTMSKRDLDQSGLSTSSGGSGVISSP 1603
            PISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHASTMSKRDLDQS L TSS GSGVISS 
Sbjct: 1469 PISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHASTMSKRDLDQSRLCTSSAGSGVISSS 1528

Query: 1604 QLAELIQNAVKEYANHGLPGEVGAFLDFVKSHFPPLRNIQVPGTSNVKGEKIIFSFDTLE 1663
            QLAELIQNAV+EYANHGLPGEVGAFLDFVKSHFPPLRNIQ PGTSNVKGEKIIFS+D LE
Sbjct: 1529 QLAELIQNAVREYANHGLPGEVGAFLDFVKSHFPPLRNIQGPGTSNVKGEKIIFSYDKLE 1588

Query: 1664 EDIYLSVRKEMHDILHSKLNEDEKFSEVLSGLVSGEDCKSVLHVKYIAALSRELAENHPT 1723
            EDIYLSVRK+MHD +HSKLNE+E FSEV    VSGED KSVLHVK++AALSRELAENH T
Sbjct: 1589 EDIYLSVRKDMHDTVHSKLNENEIFSEV----VSGEDTKSVLHVKFLAALSRELAENHST 1648

Query: 1724 SESAHNVSMPIESLQPTTFNEIGPIDCDGIYLSSCGHAVHQGCLDRYLSSLKERFARRIV 1783
            SESAH+V   I SLQPT FNEIGP DCDGIYLSSCGHAVHQGCLD YLSSLKERFARRIV
Sbjct: 1649 SESAHDVPRLIGSLQPTAFNEIGPTDCDGIYLSSCGHAVHQGCLDLYLSSLKERFARRIV 1708

Query: 1784 FEGGHIVDPEQGEFLCPVCRRLANSTLPAIPREFKKIWNPSMSSIGSLSRVSGCLNKSSE 1843
            FEGGHIVDPE+GEFLCPVCRRLANSTLPA PRE +KIWNPSM S+ SLS VSG L+K SE
Sbjct: 1709 FEGGHIVDPEKGEFLCPVCRRLANSTLPAFPRETQKIWNPSMCSVWSLSHVSGHLSKPSE 1768

Query: 1844 EVNPLYVQQAVALLQSAAKAVGKSKILKDISVHRHRKVSRNLEAVSLVLSKLYFSGKQDK 1903
            +VNPLYVQQAVALL+S AKAVGK+K+L DISVHR RKV+ NLEAVSLVLSKLYFSGK DK
Sbjct: 1769 KVNPLYVQQAVALLKSTAKAVGKNKVLTDISVHRLRKVNCNLEAVSLVLSKLYFSGKHDK 1828

Query: 1904 LISFTRVNPSILMWDTLKYSLISMETAA----------------VELKTSGGFILSLLLK 1963
            LIS +RVNPSILMWDTLKYS+ISME +A                 ELK+SGGFILSLLLK
Sbjct: 1829 LISSSRVNPSILMWDTLKYSIISMEISARSKTDMNLNIGHNTLYKELKSSGGFILSLLLK 1888

Query: 1964 VIQSIKREDSLLLLQRFCGIQRYPSHLDK--------SSCG-------------LPQFDI 2023
            VIQS+K EDSLL+LQRFCGIQR    +          +SCG             LPQFDI
Sbjct: 1889 VIQSVKCEDSLLMLQRFCGIQRLADSICSGICNEDAINSCGRGILHILTSLRVELPQFDI 1948

Query: 2024 QFLSRGSDPVLAHDPFASLMWVFFCLPCPFLSCKESLLSLVHIFYLVSVAQAIITSCIKY 2083
            Q LSRGSDPVL HDPFASLMWV FCLP PFLSCKESLLS VH+FYLVSVAQAIITSCIKY
Sbjct: 1949 QLLSRGSDPVLTHDPFASLMWVLFCLPFPFLSCKESLLSFVHMFYLVSVAQAIITSCIKY 2008

Query: 2084 QCKTDGLGFNDCLIADICKIMGESEYARRYFVSNYTEPSCNVKDMIRSLTFPYLRRCALL 2143
            + K DGLG +DCLIADICKIMGESEYAR YFVSNYTEPSCNVKDMIRSLTFPYLRRC LL
Sbjct: 2009 EGKVDGLGSSDCLIADICKIMGESEYARWYFVSNYTEPSCNVKDMIRSLTFPYLRRCTLL 2068

Query: 2144 LKLLNSSARVPIFDGETAFERYLAVDDMIDNIALELNEIEKLEKMFEIPPLDIVLKDRAS 2203
            LK+LNSSA+VPIFDGETA E  LA D+MIDNIALELNEI+KLEKMFEIPPLD V+KD AS
Sbjct: 2069 LKVLNSSAQVPIFDGETALETCLAGDNMIDNIALELNEIDKLEKMFEIPPLDAVIKDGAS 2128

Query: 2204 RSLVWKWFCHFNKEFEFQRSKNLKHCTPAVPFQLIRLPHVYHDLLQRYIKKRCPDCKHVI 2263
            RSLVWKWFCHFNKEFEFQRSKN+ HCTPAVPFQLI LPHVYHDLLQRYIKKRCPDCKHVI
Sbjct: 2129 RSLVWKWFCHFNKEFEFQRSKNIMHCTPAVPFQLIHLPHVYHDLLQRYIKKRCPDCKHVI 2188

Query: 2264 DDPALCLLCGRLCSPSWKSCCRESGCQAHANICAAGT-------EDHNLFQRSARQAPWP 2303
            DDPALCLLC RLCSPSWKSCCRESGCQAHANICAAGT           L QRSARQ+PWP
Sbjct: 2189 DDPALCLLCARLCSPSWKSCCRESGCQAHANICAAGTGVYLLIRRTTILLQRSARQSPWP 2248

BLAST of Sgr022730 vs. NCBI nr
Match: XP_022145578.1 (E3 ubiquitin-protein ligase PRT6 [Momordica charantia] >XP_022145579.1 E3 ubiquitin-protein ligase PRT6 [Momordica charantia] >XP_022145580.1 E3 ubiquitin-protein ligase PRT6 [Momordica charantia])

HSP 1 Score: 3599.7 bits (9333), Expect = 0.0e+00
Identity = 1825/2040 (89.46%), Postives = 1898/2040 (93.04%), Query Frame = 0

Query: 309  MDDMDIGSPSESVPLKPRDRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAI 368
            MDDM+IGSPSES PLKPRDRIVRRLA+LGVPE+LLDQLQPGLVSFVKDNK LI +LVSAI
Sbjct: 1    MDDMNIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60

Query: 369  LPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVG 428
            LPTDVEVAEVIRDAKAGSKKSV GPTMKNQFRESM+WLQWLMF GEPTFALKNLSKMS G
Sbjct: 61   LPTDVEVAEVIRDAKAGSKKSVAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120

Query: 429  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 488
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180

Query: 489  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 548
            AWKREGFCSKHKGAE+IQPLPEEFV+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 549  TEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKK 608
             EHKK+ANELTFAVVEMLLDFCKYSESLLSFVSKRVIS AGFLDILVRLERLLTDIVVKK
Sbjct: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300

Query: 609  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPT 668
            VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHA KKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360

Query: 669  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 728
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420

Query: 729  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 788
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480

Query: 789  LVNEAFSAA--TGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSST 848
            LVNEAFSAA  + S+EETADAMSF  +KQNP+D+DSIRHAKVGRLSQESAACNV GRSST
Sbjct: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDSIRHAKVGRLSQESAACNVFGRSST 540

Query: 849  STSASRIDEVCSDAISSTIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLY 908
             TSASR+DEVCSD ISSTIMWLTYECLKVIDSWLGTEN  GG P+MFAEN SM SSCK+Y
Sbjct: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENALGGFPNMFAENASMASSCKVY 600

Query: 909  SLRKTSALESKKMSSKVEKGKFVFEKLARKSKNYNRQYSSRMYSGLQMSIDHQHGITLGE 968
            SLRK SALES+K+SSKVEKGKF+FEK+A KSK++NRQYSSR+YSGLQMSID++HG++LGE
Sbjct: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660

Query: 969  DNQLMDVTNDTVTVEDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 1028
            DNQLMDVTNDTVTVEDYAME DALHFLS SAWPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DNQLMDVTNDTVTVEDYAMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 1029 ALRSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEV 1088
            ALRS FSESVVPS+TGASSSNLS EYVDFFKSVLTD HPYGFSAF+MEHPLRIKVFCAEV
Sbjct: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780

Query: 1089 NTGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSD 1148
            N GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+SRILERFRL D
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840

Query: 1149 YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 1208
            YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900

Query: 1209 VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIA 1268
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQIA
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960

Query: 1269 EERYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSR 1328
            EERYLRSCG SALTSQLPKWTKIYPPF GLARIATCK VLQI+RAVLFYSVFT+KSTKSR
Sbjct: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020

Query: 1329 APDSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSL 1388
            APDSVLL+ALHLLALALDICFQ+KE SDQSFCS DSIPLLLFADEEVDEGLTYG GRQSL
Sbjct: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080

Query: 1389 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYL 1448
            LSLLILLMKMHKKEGRENLLEAGSCNLSSLVE LLKKFSEIDSRCMGK++Q APEI GYL
Sbjct: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140

Query: 1449 SQSVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSVNDDTEFWQE 1508
            SQS+PSSD NSLS TSDS+K KAKARERQAAILEKMRAEQ KFLAS DSSV DD EF QE
Sbjct: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDDAEFGQE 1200

Query: 1509 SEKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEH 1568
            SEKP+VSDSAEQSETVCSLCHDSSSSVPIS+LILLQKSKLVSLIDRG VSWDQPYWRDEH
Sbjct: 1201 SEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEH 1260

Query: 1569 ASTMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSHF 1628
            AS++ +R+LDQS LSTSSGG   ISSPQLA+LIQNAVKEYA+HGLPGEVGAFLDFVKSHF
Sbjct: 1261 ASSIPRRNLDQSELSTSSGG---ISSPQLAQLIQNAVKEYASHGLPGEVGAFLDFVKSHF 1320

Query: 1629 PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKLNEDEKFSEVLSGLV 1688
            PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMH+ +HSKLNEDE  S+V S LV
Sbjct: 1321 PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENLSKVASSLV 1380

Query: 1689 SGEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPIDCDGIYLS 1748
            + ED KSVLHVKYIAALSRELAENH TSESAHNVSMP+ESLQPTTFNEI P+DCDGIYLS
Sbjct: 1381 T-EDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLS 1440

Query: 1749 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAIPRE 1808
            SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPA PRE
Sbjct: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPRE 1500

Query: 1809 FKKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQAVALLQSAAKAVGKSKILKDISVH 1868
            F+K  NPSMSSIGSLS VSG  NKS+EEVNPLYVQ+AVA LQS AKAVGK+K+LKDISVH
Sbjct: 1501 FQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVH 1560

Query: 1869 RHRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPSILMWDTLKYSLISMETAA----- 1928
            RHRKVSRNLEAVSLVLSKLYFSGKQDK IS TRVNPSILMWDTLKYSL+SME AA     
Sbjct: 1561 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTD 1620

Query: 1929 -----------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQRYPSHL------ 1988
                        ELKTSGGFILSLLLKVIQSIK EDSLLLLQRFCGIQR+   +      
Sbjct: 1621 VNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSN 1680

Query: 1989 --DKSSCG-------------LPQFDIQFLSRGSDPVLAHDPFASLMWVFFCLPCPFLSC 2048
                 SCG             LPQFDIQFLSRGSDPVLAHDPFAS+MWV FCLPCPFLSC
Sbjct: 1681 ENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSC 1740

Query: 2049 KESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGESEYARRYFVS 2108
            KESL+SLVHIFYLVSVAQ II SC+KYQ K DG+GF+DCLIADICKIMGESEYARRYFVS
Sbjct: 1741 KESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVS 1800

Query: 2109 NYTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLAVDDMIDNIA 2168
            +YTEPSCNVKDMIRSLTF YLRRCALLLKLLNSSAR PIFDGETA ERYLAVDDMIDNIA
Sbjct: 1801 DYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIA 1860

Query: 2169 LELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQRSKNLKHCTPAVPFQ 2228
            LELNEI KLEK+F+IPPLD+VLKD ASRSLVWKWFCHFN+EFEFQRSKNLKHCTPAVPFQ
Sbjct: 1861 LELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQ 1920

Query: 2229 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 2288
            LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC
Sbjct: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980

Query: 2289 AAGT-------EDHNLFQRSARQAPWPSPYLDAFGEEDLEMRRGKPLYLNEERYAALSYM 2303
            AAGT           L QRSARQAPWPSPYLDAFGEED+EMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2036

BLAST of Sgr022730 vs. NCBI nr
Match: XP_038903782.1 (E3 ubiquitin-protein ligase PRT6 isoform X1 [Benincasa hispida] >XP_038903783.1 E3 ubiquitin-protein ligase PRT6 isoform X1 [Benincasa hispida])

HSP 1 Score: 3518.8 bits (9123), Expect = 0.0e+00
Identity = 1785/2040 (87.50%), Postives = 1865/2040 (91.42%), Query Frame = 0

Query: 309  MDDMDIGSPSESVPLKPRDRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAI 368
            MDDMDIGSPSES PLKPRDRI+RRLAVLGVP++LLDQL  GLVSFVKDNKFLI +LV+AI
Sbjct: 1    MDDMDIGSPSESAPLKPRDRILRRLAVLGVPDELLDQLFRGLVSFVKDNKFLIPQLVTAI 60

Query: 369  LPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVG 428
            LP DVEV EVIRDAKAGSKKS+TGPTMK QFRESMMWLQWLMF GEP +ALKNLSKMSVG
Sbjct: 61   LPADVEVVEVIRDAKAGSKKSLTGPTMKAQFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 429  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 488
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 489  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 548
            AWKREGFCSKHKGAEQIQPLPEE VKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEELVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 549  TEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKK 608
            TEHKK+ANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 609  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPT 668
            VHELLLKLLGEPIFKYEFAKVFLNYYPTVI+EAIE+ SDH  KKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVINEAIEDSSDHVLKKYPLLPTFSVQIFTVPT 360

Query: 669  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 728
            LTPRLV EMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVVEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 729  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 788
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 789  LVNEAFSAATGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSSTST 848
            LV EAFSAA+ S E+ ADAM FQTYKQN +DIDS+RHAKVGRLSQESAACNVLG++STST
Sbjct: 481  LVKEAFSAASSSCEDAADAMYFQTYKQNVDDIDSVRHAKVGRLSQESAACNVLGKNSTST 540

Query: 849  SASRIDEVCSDAISSTIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLYSL 908
            SASR+DEVCSDAISSTIMWLTYECLK+IDSWLGTEN SG IP+M  E+ S+T SCK YS 
Sbjct: 541  SASRVDEVCSDAISSTIMWLTYECLKIIDSWLGTENVSGSIPNMLDESISLTPSCKFYSS 600

Query: 909  RKTSALESKKMSSKVEKGKFVFEKLARKSKNYNRQYSSRMYSGLQMSIDHQHGITLGEDN 968
            RK SALESKK+  K+EKGKFVFEKL R+SK +NRQYSSRMY GLQMSID++HGI LGED+
Sbjct: 601  RKMSALESKKIPYKMEKGKFVFEKLPRRSKYHNRQYSSRMYGGLQMSIDNEHGIGLGEDH 660

Query: 969  QLMDVTNDTVTVEDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 1028
            QLMDVTNDTVT EDYAME DALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL
Sbjct: 661  QLMDVTNDTVTDEDYAMEIDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 720

Query: 1029 RSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNT 1088
             SCF+ESV+PS+TGASSSNLS EYVDFFKS+LTDCHPYGFS+F+MEHPLRIKVFCAEVN 
Sbjct: 721  GSCFNESVLPSATGASSSNLSSEYVDFFKSILTDCHPYGFSSFVMEHPLRIKVFCAEVNA 780

Query: 1089 GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYL 1148
            GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRI+ERFRLSDYL
Sbjct: 781  GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRIIERFRLSDYL 840

Query: 1149 SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVK 1208
            SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQLVK
Sbjct: 841  SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQLVK 900

Query: 1209 ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAEE 1268
            ALPRDL KCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+AEE
Sbjct: 901  ALPRDLCKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVAEE 960

Query: 1269 RYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAP 1328
            RYLRSCGVSALTSQLPKWT IYPPF GLARIATCK VLQ +RAVLFYSVF E STKSRAP
Sbjct: 961  RYLRSCGVSALTSQLPKWTNIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSRAP 1020

Query: 1329 DSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLLS 1388
            DSVLLSALHLLALALDIC QQKE SDQSF + DSIPLLLFA EE+DEGL YGFGRQSLLS
Sbjct: 1021 DSVLLSALHLLALALDICLQQKESSDQSFYAQDSIPLLLFAAEEIDEGLAYGFGRQSLLS 1080

Query: 1389 LLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYLSQ 1448
            LL+LLMKMH+KEGRENLLEAGSCNLSSLVE LLKKFSEIDS CMGKVQQLAPEI GYLSQ
Sbjct: 1081 LLVLLMKMHRKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYLSQ 1140

Query: 1449 SVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSVN--DDTEFWQE 1508
            SVP+S+TN  SGTSDSEKRKAKARERQAAILEKMRAEQSKFLAS D+SVN  DD E  QE
Sbjct: 1141 SVPTSNTNRPSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVNDDDDREIGQE 1200

Query: 1509 SEKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEH 1568
            SE PNVSD AEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEH
Sbjct: 1201 SENPNVSDIAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEH 1260

Query: 1569 ASTMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSHF 1628
            AST+SKRDLDQSGLS SS GSGVISSPQLAELIQNAVKEY+NHGLPGEVGAFL+FVKSHF
Sbjct: 1261 ASTVSKRDLDQSGLSPSSAGSGVISSPQLAELIQNAVKEYSNHGLPGEVGAFLEFVKSHF 1320

Query: 1629 PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKLNEDEKFSEVLSGLV 1688
            PPLR+IQVPGTSNV+GEKIIFSFD LEEDIYLSVRKEMHD LHSK NED+K SEV     
Sbjct: 1321 PPLRDIQVPGTSNVRGEKIIFSFDKLEEDIYLSVRKEMHDTLHSKFNEDDKASEV----A 1380

Query: 1689 SGEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPIDCDGIYLS 1748
            SGED KSVLHVKYIAALSRELAENH TSES+HN+  P+ESLQPTTFNEIGP DCDGIYLS
Sbjct: 1381 SGEDSKSVLHVKYIAALSRELAENHSTSESSHNIHTPVESLQPTTFNEIGPTDCDGIYLS 1440

Query: 1749 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAIPRE 1808
            SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPA PRE
Sbjct: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPRE 1500

Query: 1809 FKKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQAVALLQSAAKAVGKSKILKDISVH 1868
             +KIWNP+ SS+GS S VSG LNKSSE+VNPLY+QQAVALLQ AA AVGK+K+LKDISVH
Sbjct: 1501 LQKIWNPATSSVGSSSHVSGHLNKSSEKVNPLYIQQAVALLQYAANAVGKNKVLKDISVH 1560

Query: 1869 RHRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPSILMWDTLKYSLISMETAA----- 1928
            RHRKVSRNLEAVSLVLSKLYFSGKQDKLIS +RVNPSILMWDTLKYSLISME AA     
Sbjct: 1561 RHRKVSRNLEAVSLVLSKLYFSGKQDKLISSSRVNPSILMWDTLKYSLISMEIAARSKTD 1620

Query: 1929 -----------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQRYPSHL------ 1988
                        ELK SGGF+LSLLLKVIQS+K EDSLLLLQRFCGIQR+   +      
Sbjct: 1621 MNPNIGINTLYKELKISGGFVLSLLLKVIQSVKCEDSLLLLQRFCGIQRFADSICSGTSN 1680

Query: 1989 --DKSSCG-------------LPQFDIQFLSRGSDPVLAHDPFASLMWVFFCLPCPFLSC 2048
                 SCG             LPQFDIQFLSRGSDPVLAHDPFASLMWV FCLP PFLSC
Sbjct: 1681 ENASDSCGRGILHILTSLHVELPQFDIQFLSRGSDPVLAHDPFASLMWVMFCLPFPFLSC 1740

Query: 2049 KESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGESEYARRYFVS 2108
            +ESLLSLVHIFYLVSVAQAIITS IK QCK DGLG +DCLIADICKIMGESE AR YFVS
Sbjct: 1741 RESLLSLVHIFYLVSVAQAIITSFIKSQCKVDGLGSSDCLIADICKIMGESECARLYFVS 1800

Query: 2109 NYTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLAVDDMIDNIA 2168
            NYTEPSCNVKDMIRSLTFPYLRRCALLLKLL+SSARVPIF GETA E YLA +++IDNI 
Sbjct: 1801 NYTEPSCNVKDMIRSLTFPYLRRCALLLKLLSSSARVPIFYGETALETYLAGNNIIDNIT 1860

Query: 2169 LELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQRSKNLKHCTPAVPFQ 2228
            +ELNEIEKLEKMFEIPPLD VLKD  SR  V KWFCHF+KEFEFQRSK +KHCTPAVPFQ
Sbjct: 1861 VELNEIEKLEKMFEIPPLDTVLKDGTSRLFVSKWFCHFDKEFEFQRSKIIKHCTPAVPFQ 1920

Query: 2229 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 2288
            LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLC+PSWKSCCRESGCQAHAN+C
Sbjct: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCTPSWKSCCRESGCQAHANVC 1980

Query: 2289 AAGT-------EDHNLFQRSARQAPWPSPYLDAFGEEDLEMRRGKPLYLNEERYAALSYM 2303
            AAGT           L QRSARQAPWPSPYLDAFGEED EMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDTEMRRGKPLYLNEERYAALSYM 2036

BLAST of Sgr022730 vs. NCBI nr
Match: XP_008442546.1 (PREDICTED: E3 ubiquitin-protein ligase PRT6 isoform X1 [Cucumis melo] >XP_008442547.1 PREDICTED: E3 ubiquitin-protein ligase PRT6 isoform X1 [Cucumis melo])

HSP 1 Score: 3482.2 bits (9028), Expect = 0.0e+00
Identity = 1762/2039 (86.41%), Postives = 1854/2039 (90.93%), Query Frame = 0

Query: 309  MDDMDIGSPSESVPLKPRDRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAI 368
            MDDMDIGSPSES PLKPRDRI+RRLAVLGVP++LLDQL  GLV FVKDNKFLI +LVSAI
Sbjct: 1    MDDMDIGSPSESTPLKPRDRILRRLAVLGVPDELLDQLFRGLVIFVKDNKFLIPQLVSAI 60

Query: 369  LPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVG 428
            LPTDVEV EVIRDA  GSKK++TGPTMK  FRESMMWLQWLMF GEP +ALKNLSKMSVG
Sbjct: 61   LPTDVEVVEVIRDAIPGSKKTLTGPTMKAHFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 429  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 488
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 489  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 548
            AWKREGFCSKHKGAEQIQPLPEE+VKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 549  TEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKK 608
            TEHKK+ANELTFAVVEMLLDFCK+SESLLSFVSKRVISSAG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 609  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPT 668
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHA KKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 669  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 728
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 729  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 788
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 789  LVNEAFSAATGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSSTST 848
            LV EAFSAA+ S E+TAD M FQTYKQN +DIDS+RHAKVGRLSQESAAC VLG+SSTST
Sbjct: 481  LVKEAFSAASSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVLGKSSTST 540

Query: 849  SASRIDEVCSDAISSTIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLYSL 908
            SASR+DEVCSDAISSTIMWLTYECLK+ID+WLGTEN SG IP+M  E+ S   SCK YS 
Sbjct: 541  SASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAPSCKFYSS 600

Query: 909  RKTSALESKKMSSKVEKGKFVFEKLARKSKNYNRQYSSRMYSGLQMSIDHQHGITLGEDN 968
            RKTSAL SKKMS K+EKGKF+F+KL+R+SK +NRQYSSRMYSGLQMSID++HGI+LGEDN
Sbjct: 601  RKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGEDN 660

Query: 969  QLMDVTNDTVTVEDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 1028
             LMDVTNDTVT EDYAME DALHFLSLS+WPNIVYDVSSQDISIHIPLHRLLSLLLQKAL
Sbjct: 661  HLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 720

Query: 1029 RSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNT 1088
             SCFSES VPS+TGASSSNLS EYVDFFKSVLTDCHPYGFS+F+MEHPLRIKVFCAEVN 
Sbjct: 721  SSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEVNA 780

Query: 1089 GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYL 1148
            GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYV RILERFRLSDYL
Sbjct: 781  GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCRILERFRLSDYL 840

Query: 1149 SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVK 1208
            SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQLVK
Sbjct: 841  SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQLVK 900

Query: 1209 ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAEE 1268
            ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+AEE
Sbjct: 901  ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVAEE 960

Query: 1269 RYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAP 1328
            RYLRSCGVSALTSQLPKWTKIYPPF GLARIATCK VLQ +RAVLFYSVF E STKSRAP
Sbjct: 961  RYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSRAP 1020

Query: 1329 DSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLLS 1388
            DSVLLSALHLLALALD+CFQQKE  DQSF + DSIPLLLFA EE++EGL YGFGRQSLLS
Sbjct: 1021 DSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGLAYGFGRQSLLS 1080

Query: 1389 LLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYLSQ 1448
            LLILLMKMHKKEGRENLLEAGSCNLSSLVE LLKKFSEIDS CMGKVQQLAPEI GYLSQ
Sbjct: 1081 LLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYLSQ 1140

Query: 1449 SVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSV-NDDTEFWQES 1508
            SVP+S T+  + TSDSEKRKAKARERQAAILEKMRAEQSKFLAS D+SV +DDTEF QE 
Sbjct: 1141 SVPTSTTSRPAETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQEP 1200

Query: 1509 EKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1568
            EKPNVSD AEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA
Sbjct: 1201 EKPNVSDDAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1260

Query: 1569 STMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSHFP 1628
            ST SKRD+DQSG+STSS GS VISSPQLAELIQNAVKEY NHGLPGEVGAFLDF+KSHFP
Sbjct: 1261 STTSKRDIDQSGVSTSSAGSVVISSPQLAELIQNAVKEYTNHGLPGEVGAFLDFLKSHFP 1320

Query: 1629 PLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKLNEDEKFSEVLSGLVS 1688
            PLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMHD LHSK +E EK S V     S
Sbjct: 1321 PLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVFKEMHDTLHSKFHEGEKISTV----AS 1380

Query: 1689 GEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPIDCDGIYLSS 1748
            GED +SVLHVKYIAALSRELAEN  TSESA NV MP+ES QPT  NEIGPIDCDGIYLSS
Sbjct: 1381 GEDSRSVLHVKYIAALSRELAENRSTSESARNVHMPVESSQPTILNEIGPIDCDGIYLSS 1440

Query: 1749 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAIPREF 1808
            CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPA PREF
Sbjct: 1441 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPREF 1500

Query: 1809 KKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQAVALLQSAAKAVGKSKILKDISVHR 1868
            +KIWNP  SS+G+LS VSG LNKS+E VNPLY+Q+AVALLQSAAKAVGK+ +LKDISVHR
Sbjct: 1501 QKIWNPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISVHR 1560

Query: 1869 HRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPSILMWDTLKYSLISMETAA------ 1928
            H+KVSRNLEAVSLVLSKLYFS KQDKLIS +RVNPSILMWDTLKYSL+SME AA      
Sbjct: 1561 HKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKTDM 1620

Query: 1929 ----------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQRYPSHL------- 1988
                       ELKTSGGF+LSLLLKVI S+K EDSLLLLQR CGIQR+   +       
Sbjct: 1621 NPSIGLNTLYKELKTSGGFVLSLLLKVIHSVKCEDSLLLLQRLCGIQRFADSICSGMSNE 1680

Query: 1989 -DKSSCG-------------LPQFDIQFLSRGSDPVLAHDPFASLMWVFFCLPCPFLSCK 2048
                SCG             LP FD QFLSRGSDPV+AHDPFASLMWV FCLP PFLSC+
Sbjct: 1681 NASDSCGRGILHILTSLRSELPHFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLSCR 1740

Query: 2049 ESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGESEYARRYFVSN 2108
            ESLLSLVHIFYLVSV QAIIT   K QCK DGLG +DCLI DICKIMGESEYAR+YFVSN
Sbjct: 1741 ESLLSLVHIFYLVSVTQAIITYFTKSQCKVDGLGSSDCLITDICKIMGESEYARQYFVSN 1800

Query: 2109 YTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLAVDDMIDNIAL 2168
            YTEPSCN+KD +RSLTFP+LRRCALLL+LL+SSARVPIFDGETA E YL  ++MIDNI +
Sbjct: 1801 YTEPSCNIKDTVRSLTFPFLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNITV 1860

Query: 2169 ELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQRSKNLKHCTPAVPFQL 2228
            ELNE+EKL+KMF+IPPLDIVLKD  SR LV KWFCHFNKEFEFQRSK +KHCTPAVPFQL
Sbjct: 1861 ELNEVEKLQKMFDIPPLDIVLKDGTSRLLVSKWFCHFNKEFEFQRSKIIKHCTPAVPFQL 1920

Query: 2229 IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA 2288
            IRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANICA
Sbjct: 1921 IRLPHVYHDLLQRYIKKRCTDCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANICA 1980

Query: 2289 AGT-------EDHNLFQRSARQAPWPSPYLDAFGEEDLEMRRGKPLYLNEERYAALSYM 2303
            AGT           L QRSARQAPWPSPYLDAFGEED+EMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2035

BLAST of Sgr022730 vs. NCBI nr
Match: KAA0044092.1 (E3 ubiquitin-protein ligase PRT6 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 3482.2 bits (9028), Expect = 0.0e+00
Identity = 1762/2039 (86.41%), Postives = 1854/2039 (90.93%), Query Frame = 0

Query: 309  MDDMDIGSPSESVPLKPRDRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAI 368
            MDDMDIGSPSES PLKPRDRI+RRLAVLGVP++LLDQL  GLV FVKDNKFLI +LVSAI
Sbjct: 1    MDDMDIGSPSESTPLKPRDRILRRLAVLGVPDELLDQLFRGLVIFVKDNKFLIPQLVSAI 60

Query: 369  LPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVG 428
            LPTDVEV EVIRDA  GSKK++TGPTMK  FRESMMWLQWLMF GEP +ALKNLSKMSVG
Sbjct: 61   LPTDVEVVEVIRDAIPGSKKTLTGPTMKAHFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 429  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 488
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 489  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 548
            AWKREGFCSKHKGAEQIQPLPEE+VKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 549  TEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKK 608
            TEHKK+ANELTFAVVEMLLDFCK+SESLLSFVSKRVISSAG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 609  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPT 668
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHA KKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 669  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 728
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 729  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 788
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 789  LVNEAFSAATGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSSTST 848
            LV EAFSAA+ S E+TAD M FQTYKQN +DIDS+RHAKVGRLSQESAAC VLG+SSTST
Sbjct: 481  LVKEAFSAASSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVLGKSSTST 540

Query: 849  SASRIDEVCSDAISSTIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLYSL 908
            SASR+DEVCSDAISSTIMWLTYECLK+ID+WLGTEN SG IP+M  E+ S   SCK YS 
Sbjct: 541  SASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAPSCKFYSS 600

Query: 909  RKTSALESKKMSSKVEKGKFVFEKLARKSKNYNRQYSSRMYSGLQMSIDHQHGITLGEDN 968
            RKTSAL SKKMS K+EKGKF+F+KL+R+SK +NRQYSSRMYSGLQMSID++HGI+LGEDN
Sbjct: 601  RKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGEDN 660

Query: 969  QLMDVTNDTVTVEDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 1028
             LMDVTNDTVT EDYAME DALHFLSLS+WPNIVYDVSSQDISIHIPLHRLLSLLLQKAL
Sbjct: 661  HLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 720

Query: 1029 RSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNT 1088
             SCFSES VPS+TGASSSNLS EYVDFFKSVLTDCHPYGFS+F+MEHPLRIKVFCAEVN 
Sbjct: 721  SSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEVNA 780

Query: 1089 GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYL 1148
            GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYV RILERFRLSDYL
Sbjct: 781  GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCRILERFRLSDYL 840

Query: 1149 SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVK 1208
            SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQLVK
Sbjct: 841  SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQLVK 900

Query: 1209 ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAEE 1268
            ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+AEE
Sbjct: 901  ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVAEE 960

Query: 1269 RYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAP 1328
            RYLRSCGVSALTSQLPKWTKIYPPF GLARIATCK VLQ +RAVLFYSVF E STKSRAP
Sbjct: 961  RYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSRAP 1020

Query: 1329 DSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLLS 1388
            DSVLLSALHLLALALD+CFQQKE  DQSF + DSIPLLLFA EE++EGL YGFGRQSLLS
Sbjct: 1021 DSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGLAYGFGRQSLLS 1080

Query: 1389 LLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYLSQ 1448
            LLILLMKMHKKEGRENLLEAGSCNLSSLVE LLKKFSEIDS CMGKVQQLAPEI GYLSQ
Sbjct: 1081 LLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYLSQ 1140

Query: 1449 SVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSV-NDDTEFWQES 1508
            SVP+S T+  + TSDSEKRKAKARERQAAILEKMRAEQSKFLAS D+SV +DDTEF QE 
Sbjct: 1141 SVPTSTTSRPAETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQEP 1200

Query: 1509 EKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1568
            EKPNVSD AEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA
Sbjct: 1201 EKPNVSDDAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1260

Query: 1569 STMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSHFP 1628
            ST SKRD+DQSG+STSS GS VISSPQLAELIQNAVKEY NHGLPGEVGAFLDF+KSHFP
Sbjct: 1261 STTSKRDIDQSGVSTSSAGSVVISSPQLAELIQNAVKEYTNHGLPGEVGAFLDFLKSHFP 1320

Query: 1629 PLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKLNEDEKFSEVLSGLVS 1688
            PLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMHD LHSK +E EK S V     S
Sbjct: 1321 PLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVFKEMHDTLHSKFHEGEKISTV----AS 1380

Query: 1689 GEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPIDCDGIYLSS 1748
            GED +SVLHVKYIAALSRELAEN  TSESA NV MP+ES QPT  NEIGPIDCDGIYLSS
Sbjct: 1381 GEDSRSVLHVKYIAALSRELAENRSTSESARNVHMPVESSQPTILNEIGPIDCDGIYLSS 1440

Query: 1749 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAIPREF 1808
            CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPA PREF
Sbjct: 1441 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPREF 1500

Query: 1809 KKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQAVALLQSAAKAVGKSKILKDISVHR 1868
            +KIWNP  SS+G+LS VSG LNKS+E VNPLY+Q+AVALLQSAAKAVGK+ +LKDISVHR
Sbjct: 1501 QKIWNPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISVHR 1560

Query: 1869 HRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPSILMWDTLKYSLISMETAA------ 1928
            H+KVSRNLEAVSLVLSKLYFS KQDKLIS +RVNPSILMWDTLKYSL+SME AA      
Sbjct: 1561 HKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKTDM 1620

Query: 1929 ----------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQRYPSHL------- 1988
                       ELKTSGGF+LSLLLKVI S+K EDSLLLLQR CGIQR+   +       
Sbjct: 1621 NPSIGLNTLYKELKTSGGFVLSLLLKVIHSVKCEDSLLLLQRLCGIQRFADSICSGMSNE 1680

Query: 1989 -DKSSCG-------------LPQFDIQFLSRGSDPVLAHDPFASLMWVFFCLPCPFLSCK 2048
                SCG             LP FD QFLSRGSDPV+AHDPFASLMWV FCLP PFLSC+
Sbjct: 1681 NASDSCGRGILHILTSLRSELPHFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLSCR 1740

Query: 2049 ESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGESEYARRYFVSN 2108
            ESLLSLVHIFYLVSV QAIIT   K QCK DGLG +DCLI DICKIMGESEYAR+YFVSN
Sbjct: 1741 ESLLSLVHIFYLVSVTQAIITYFTKSQCKVDGLGSSDCLITDICKIMGESEYARQYFVSN 1800

Query: 2109 YTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLAVDDMIDNIAL 2168
            YTEPSCN+KD +RSLTFP+LRRCALLL+LL+SSARVPIFDGETA E YL  ++MIDNI +
Sbjct: 1801 YTEPSCNIKDTVRSLTFPFLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNITV 1860

Query: 2169 ELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQRSKNLKHCTPAVPFQL 2228
            ELNE+EKL+KMF+IPPLDIVLKD  SR LV KWFCHFNKEFEFQRSK +KHCTPAVPFQL
Sbjct: 1861 ELNEVEKLQKMFDIPPLDIVLKDGTSRLLVSKWFCHFNKEFEFQRSKIIKHCTPAVPFQL 1920

Query: 2229 IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA 2288
            IRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANICA
Sbjct: 1921 IRLPHVYHDLLQRYIKKRCTDCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANICA 1980

Query: 2289 AGT-------EDHNLFQRSARQAPWPSPYLDAFGEEDLEMRRGKPLYLNEERYAALSYM 2303
            AGT           L QRSARQAPWPSPYLDAFGEED+EMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2035

BLAST of Sgr022730 vs. ExPASy Swiss-Prot
Match: F4KCC2 (E3 ubiquitin-protein ligase PRT6 OS=Arabidopsis thaliana OX=3702 GN=PRT6 PE=1 SV=1)

HSP 1 Score: 1757.7 bits (4551), Expect = 0.0e+00
Identity = 1014/2055 (49.34%), Postives = 1318/2055 (64.14%), Query Frame = 0

Query: 327  DRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAILPTDVEVAEVIRDAKAGS 386
            D ++ RLA +GVP+    + + GLV FV+ N   I+ELVSA+LPTD +V   +++A+   
Sbjct: 17   DLVIERLASVGVPKKY--RSKRGLVEFVRANPAKISELVSALLPTDDDVKLGLKEARERP 76

Query: 387  KKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVGQRGVCGAVWGHNDIAYRC 446
            +KS   PTMK +FRESM  LQWLMF  EP  +L+NL+K+++ QRGVCG+VWG NDIAYRC
Sbjct: 77   RKSAVSPTMKKRFRESMNMLQWLMFQDEPDVSLRNLAKLNLDQRGVCGSVWGQNDIAYRC 136

Query: 447  RTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVTAWKREGFCSKHKGAEQIQ 506
            RTCE+DPTCAICVPCFQ+G+H  HDYSIIYTGGGCCDCGD TAWK +GFCS HKG+EQI+
Sbjct: 137  RTCENDPTCAICVPCFQNGDHNSHDYSIIYTGGGCCDCGDETAWKPDGFCSNHKGSEQIR 196

Query: 507  PLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRVTEHKKIANELTFAVVEML 566
            PL E    SVGPILDALFT W NKLLSAE    +  + +D +   +K++NELTF VVEML
Sbjct: 197  PLSENLANSVGPILDALFTCWNNKLLSAESSGQKGARSNDTLVILQKMSNELTFIVVEML 256

Query: 567  LDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKKVHELLLKLLGEPIFKYEF 626
            L+F   SESLLSFVS+R+ISS+G L IL++ ER L   V+KK+H+L LKL+G+P+FK EF
Sbjct: 257  LEFSMSSESLLSFVSRRIISSSGLLSILLKAERFLDQDVMKKLHDLFLKLIGDPVFKCEF 316

Query: 627  AKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPTLTPRLVEEMNLLSILLGC 686
            AK F++YYP VISE +++G+D+AFKKYPLL TFSVQI TVPTLTP LV+EMNLL++LLGC
Sbjct: 317  AKAFVSYYPVVISEVVKQGTDNAFKKYPLLSTFSVQILTVPTLTPFLVKEMNLLAMLLGC 376

Query: 687  LEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAVVPRYVIYEQQDILRTWLR 746
            L DIF+SC  EDG LQ  K   L ET+ RV+ D++FVMSHA+V +Y  +E +++ R+WL 
Sbjct: 377  LSDIFVSCSGEDGLLQATKLERLCETSERVIGDLKFVMSHAIVSKYATHEHRELSRSWLT 436

Query: 747  LLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSLLVNEAFSAATGSTEETAD 806
            LLTF QGM+P KRETG+ I+EEN+ +HL F L HS+A IHSLLVN  +SAA+    E  D
Sbjct: 437  LLTFAQGMNPLKRETGIPIDEENDYMHLFFVLGHSIAVIHSLLVNGTYSAASDEEIEN-D 496

Query: 807  AMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSST--STSASRIDEV--CSDAIS 866
              + + + +   D D  R+AKVGRLS E + C  +  SS+  S+ AS + ++      + 
Sbjct: 497  RNAKEEFDKC--DGDGERYAKVGRLSHEDSVCTAIVSSSSFDSSMASEVHKIDPFHALLP 556

Query: 867  STIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLYSLRKTSALESKKMSSK 926
            S+ ++L  ECLKV+++ LG +    GI             CKL S    +  ESK    +
Sbjct: 557  SSAIYLIRECLKVLETCLGNDE---GISKFL---------CKLSSSSGRNIPESKMSWPR 616

Query: 927  -----VEKGKFVFEKLARKSKNYNRQYSSRMYSGLQ--MSIDH---QHGITLGEDNQLMD 986
                 VE G  V   LA  S++ +   S  +   +Q  +S+D+    +G+         D
Sbjct: 617  RDLLNVETGGSVSSNLASSSRDPSTGLSP-LCGDIQTNLSLDNVCGPYGVV------QTD 676

Query: 987  VTNDTVTVE----DYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 1046
            VT D+  V     D       L  L L  WP+I YDVSSQ IS+H+PLHRLLSLL+QKAL
Sbjct: 677  VTADSKRVSCNSADLTKNASGLRILGLCDWPDIHYDVSSQAISVHLPLHRLLSLLIQKAL 736

Query: 1047 RSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNT 1106
            R C+ ES   S  G S S+  + + DFF SV+ D HP GFSA +MEH L+I+VFCA+V  
Sbjct: 737  RICYGESA--SYNGVSISH-EIPHADFFSSVIGDFHPCGFSALVMEHVLQIRVFCAQVIA 796

Query: 1107 GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYL 1166
            GMW++NGD+AL+SCE YRS+RWSEQ LELDLFLLQCCAA+AP D YV ++L RF LS YL
Sbjct: 797  GMWKKNGDSALVSCEWYRSVRWSEQGLELDLFLLQCCAALAPADSYVDKLLSRFGLSSYL 856

Query: 1167 SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVK 1226
            SLN +  +EYEP+LVQEML L+IQ++ ERRFCGL+ AESL+RE+I+KLA GD THSQLVK
Sbjct: 857  SLNPDITNEYEPVLVQEMLGLLIQILQERRFCGLSTAESLRREIIFKLATGDFTHSQLVK 916

Query: 1227 ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAEE 1286
            +LPRDLSK  +LQE+LD ++VY NPSG NQG YSL    WKELDLYHPRW  RDLQ AEE
Sbjct: 917  SLPRDLSKSDELQEVLDDVSVYCNPSGMNQGKYSLQSSCWKELDLYHPRWQSRDLQSAEE 976

Query: 1287 RYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAP 1346
            R+ R CGVSALT+QLP+W  IYPP  GLARI TCK   QI+ + L+Y++ +  S KSRAP
Sbjct: 977  RFSRYCGVSALTTQLPRWRMIYPPLKGLARIGTCKATFQIISSALYYALQSGTSVKSRAP 1036

Query: 1347 DSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLLS 1406
            D VL++AL LL+L+LDIC QQ++ + Q  C  +SIP+L  A  E+  G+  G  ++SLLS
Sbjct: 1037 DGVLITALQLLSLSLDICTQQRQSNSQDCCLENSIPILELAGLEI-IGIAQGTEKESLLS 1096

Query: 1407 LLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYLSQ 1466
            LL+ LMK    +GR    E GSCN+SS +  LLKKFS IDS CM  +Q LAPE+ G    
Sbjct: 1097 LLVSLMKTRMGDGRHQFPEPGSCNISSWIGNLLKKFSAIDSVCMNLLQSLAPEVVG---- 1156

Query: 1467 SVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSVNDD---TEFWQ 1526
               S     +SG++  EKRKAKA+ERQAAI+ KM+AEQSKFL++  SS++DD   +EF +
Sbjct: 1157 --QSGFDKVMSGSTSDEKRKAKAKERQAAIMAKMKAEQSKFLSTLSSSMDDDDPRSEF-E 1216

Query: 1527 ESEKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDE 1586
             S+     DS      VCSLCHD  S  P+SFLI LQKSKL+S +DRGP SWDQ    ++
Sbjct: 1217 TSDSVMEHDSEIAVREVCSLCHDPDSKDPVSFLIFLQKSKLLSFVDRGPPSWDQCPQSEK 1276

Query: 1587 HASTMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSH 1646
              S     DL +   S+ S     ISSP + +L  + + E AN          ++ +K+ 
Sbjct: 1277 KISVDGAPDLLRMNASSDSLR---ISSPLMLQLSDDTISESAN---------MIESIKAR 1336

Query: 1647 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKL-------NEDEKF 1706
               + N Q    S+    K   + ++LE  +Y +VR ++ ++++  L       +E E  
Sbjct: 1337 L--IGNGQTEKRSSDGRGKDESNMESLEIAMYQTVRNKIENMINQSLTRVDHQPHEAENC 1396

Query: 1707 SEVLSGLVSGEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPI 1766
            SE  S  V G    S L  ++    SR+ +          +                 PI
Sbjct: 1397 SEKNS--VGG---PSTLQGRFPDIRSRQTSRRPDAGSDGFH-----------------PI 1456

Query: 1767 DCDGIYLSSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANS 1826
            DCDG+YLSSCGHAVHQ CL+RYL SLKER  RR VFEG HIVD ++ EFLCPVCRRLANS
Sbjct: 1457 DCDGVYLSSCGHAVHQSCLERYLKSLKERSGRRTVFEGAHIVDLKKKEFLCPVCRRLANS 1516

Query: 1827 TLPAIPREFKKIWNPSMSSIGSLSRVSGCLNKSSEEVNP-LYVQQAVALLQSAAKAVGKS 1886
             LP  P +        + S+  L        +  + + P L++ +A+ LL+SAA+ +   
Sbjct: 1517 VLPECPGD--------LCSVSKLQDSPRTKLRRKDALQPSLWLSEALCLLRSAAEVIEDG 1576

Query: 1887 KILKDISVHRHRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNP-SILMWDTLKYSLIS 1946
               K ++        ++L++VS +L   YF   +DK +    + P SI+MWDTLKYSLIS
Sbjct: 1577 DRGKTVTPQGDGPRRKDLKSVSKMLWDFYFPKPEDKTLKRLWLPPQSIVMWDTLKYSLIS 1636

Query: 1947 METAA-----------------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQR 2006
            ME                     ELKTS G ILS+LL+V+QS + ++++ + QRF G++ 
Sbjct: 1637 MEIGTRFAKNSMLPVYCIDSLYEELKTSKGTILSVLLRVVQSSRTKNTIHVRQRFVGMK- 1696

Query: 2007 YPSHLDKSSC-------------------GLPQFDIQFLSRGSDPVLAHDPFASLMWVFF 2066
               HL +S C                    L   D+ + +R SDPVLAHDPF+SLMW  F
Sbjct: 1697 ---HLAESICYGVSSSSSSSIFGSEGTTGSLKNIDLLW-NRASDPVLAHDPFSSLMWALF 1756

Query: 2067 CLPCPFLSCKESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGES 2126
            CLP PFL+C+ESLLSLVHIF+ VS+ Q +I  C     +   L F + L+ DI   + ES
Sbjct: 1757 CLPFPFLTCEESLLSLVHIFHSVSLVQTVIAYCACRPSELSELNFGENLLNDISNALRES 1816

Query: 2127 EYARRYFVSNYTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLA 2186
                 YF SN  + SC++KD IR  + P+LRRCALL KLL S+ R  + +    F+  L 
Sbjct: 1817 G-GWEYFRSNNMDLSCDIKDTIRKYSLPFLRRCALLWKLLKSTPR-KLHEESDMFD--LP 1876

Query: 2187 VDDMIDNIAL------ELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQ 2246
             D   DN+        ELN +++LEKMF IPP+DI+L D   RS    W  HF +E+   
Sbjct: 1877 SDPTTDNMDFIYSPQSELNHVQELEKMFNIPPIDIILNDELLRSSTQIWLQHFQREYRVN 1936

Query: 2247 RSKNLKHCTPAVPFQLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWK 2303
            R K     TP VPFQL++LP++Y DLLQR IKKRC +C  VI++P LCLLCG LCSP W 
Sbjct: 1937 RVKRSLCITPVVPFQLMKLPNLYQDLLQRCIKKRCVNCTKVIEEPVLCLLCGSLCSPIWS 1983

BLAST of Sgr022730 vs. ExPASy Swiss-Prot
Match: F1QJX5 (E3 ubiquitin-protein ligase ubr3 OS=Danio rerio OX=7955 GN=ubr3 PE=2 SV=2)

HSP 1 Score: 205.7 bits (522), Expect = 5.8e-51
Identity = 438/2021 (21.67%), Postives = 775/2021 (38.35%), Query Frame = 0

Query: 401  ESMMWLQWLMFGGEPTFALKNLSKMSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVP 460
            E++ W +WL+ GG+  F     +  S     +CG VW  N +AYRCRTC   P  ++C  
Sbjct: 51   EALDWCKWLLAGGD-GFDEFCRTVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAE 110

Query: 461  CFQSGNHKDHDYSIIYT-GGGCCDCGDVTAWKREGFCSKH--KGAEQIQPLPEEFVKSVG 520
            CF +G+H  HD+++  +  GG CDCGD    +  GFC++H  K  E +  +P + +    
Sbjct: 111  CFNNGDHTGHDFNMFRSQAGGACDCGDGNVMRESGFCNRHRLKTGENVPSVPRDLLLMSE 170

Query: 521  PILDALFTSWKNKLLSAEDISVEDPKLSDRVTEHKKIANELTFAVVEMLLDFCKYSESLL 580
             +L     +    L    D   E    +DR  + +K+  +L    +  L +  K   ++ 
Sbjct: 171  MVLPRFIITIIQYL---RDGYTEPESAADR--DLQKVLQQLD-PHISFLEELTKMGGAMR 230

Query: 581  SFVSKRVISSAGFLDILVRLERLLTDIVVKKVHELLLKLLGEPIFKYEFAKVFLNYYPTV 640
            + ++K +     F ++ +  E    ++  KK +E  L  L         +   ++     
Sbjct: 231  TVLTKILTDQQTFKELSMGQE---DNVYAKKNYEKYLSALK--------SSGLVSVEEKG 290

Query: 641  ISEAIEEGSDHAFKKYPLLPTFSVQIFTVPTLTPRLVEEMNLLSILLGCLEDIFISCVSE 700
             +    +G+  A      L             + +  ++  L  +  G  + + +S  ++
Sbjct: 291  AAGGAGDGTSDAAAGAGALSLLGATATASLDDSSKEEDQDGLQGV--GQRKRVKLSSSTK 350

Query: 701  DGRLQVIKWSNLYETTIRVVEDIRFVMSHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQ 760
            D  +        +      +E++ F       P+              +++TF+  M P 
Sbjct: 351  DPSIMDTLKHKCF------LEELLFWTIKYEFPQ--------------KMVTFLLNMLPD 410

Query: 761  KRETGLHIEEENENVHLPFGLDHSVANIHSLLVNEAFSAATGSTEETADAMSFQTYKQNP 820
            +                    D+ +    + + + AF   T      +D MS        
Sbjct: 411  Q--------------------DYKITFTKTFVQHYAFIMKTLMKSHESDTMS-------- 470

Query: 821  EDIDSIRHAKVGRLSQESAACNVLGRSSTSTSASRIDEVCS--DAISSTIMWLTYECLKV 880
               + I H  V   S E               A  + E C   D + + ++++   CL  
Sbjct: 471  ---NRIVHISVQLFSNEEL-------------ARHVTEECQLLDIMVTVLLYMMESCL-- 530

Query: 881  IDSWLGTENTSGGI-----PDMFAENT---SMTSSCKLYSLRKTSALESKKMSSKVEKGK 940
            I S L  E  +  +       +   NT    ++    + S +  +    +  S  +    
Sbjct: 531  IKSELQDEENNRHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHSLLLLWMS 590

Query: 941  FV--FE--KLARKSKNYNRQYSSRMY---SGLQMSIDHQHGITLGEDNQLMDVTNDTVTV 1000
            FV  F+   L ++  N + ++ S+ Y      ++    Q    L    ++ +  + T TV
Sbjct: 591  FVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLTHCKVKETQDYTKTV 650

Query: 1001 EDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKALRSCFSESVVPSS 1060
              Y +E   + F ++      V + S   ++ H+PLHR  ++ L K              
Sbjct: 651  VRYCLETLQMWFDAI----GFVDEPSLNQLTFHLPLHRYYAMFLSK-------------- 710

Query: 1061 TGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNTGMWRRNGDAALL 1120
             G     L L+      S+L D         +M HPL+I+   +E+++ MW RNG     
Sbjct: 711  -GVKCQGLDLD------SLLPD---QEMLMKIMVHPLQIQASLSEIHSNMWVRNGLQIKG 770

Query: 1121 SCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYLSL-----NVERP 1180
                Y    +    ++ D++LLQ CA+   PD ++S + ERF++ D L++     N    
Sbjct: 771  QAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNAVLD 830

Query: 1181 SEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPRD-- 1240
            SE E  +++  LT ++ + + R   G++  E L+ E++ +L + D THS L+  +P +  
Sbjct: 831  SEQERPMLEGALTFLVILCSLRIHLGMSDDEILRAEMVSQLCMNDRTHSSLLDLIPENPN 890

Query: 1241 -----LSKCHQLQEILDTIAVYSNP-----SGFNQGMYSLSWKYW-KELD---LYHPRWS 1300
                 +      +E+L  +A +  P         QGMY+   + W KE D   +      
Sbjct: 891  PKSGVVPGSCSFEEMLSGVADFKAPVFEPGGSMQQGMYTPKAEVWEKEFDPIMVILRTVY 950

Query: 1301 LRDLQIAEERY---LRSCGVSALTSQLP--KWTKIYPPFGGLARIATCKVVLQIVRAVLF 1360
             RD+Q A +RY   L+  GV       P  + T ++P + GL R+  CK  L IV   L 
Sbjct: 951  RRDVQSAMDRYSAFLKQSGVHTGNPWPPYKERTPLHPCYKGLVRLLHCK-TLHIVIFTLL 1010

Query: 1361 YSVFTEKSTKSRAPDSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVD 1420
            Y ++ +    S   + VL   L+L+ L LD   Q  ++ ++        P +   +E   
Sbjct: 1011 YKIWMDHQNMS---EHVLCMVLYLIELGLDNQVQDDKVEEE--------PCI---EEHCH 1070

Query: 1421 EGLTYGFGRQSLLSLLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGK 1480
            +     F   SLLS                       NL  ++      F  +      +
Sbjct: 1071 DS---WFPGTSLLS-----------------------NLHHVI-----NFVRV------R 1130

Query: 1481 VQQLAPEI----FGYLSQSVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMR--AEQSK 1540
            V + APE+         +  P S T+S S T     R+A+     A    K +    +S 
Sbjct: 1131 VPETAPEVERKRERERERETPPS-TSSESATFGQNLREAQVFSLVAERRRKFQEIINRSN 1190

Query: 1541 FLASFDSSVNDDTEFWQESEKPN--VSDSAEQSETVCSL---CHD--SSSSVPISFLILL 1600
              AS           W     P   V++  E  E++ SL    H   S+    +S   L 
Sbjct: 1191 HEASQAVRPKSSASRWLPPGTPPQLVTEILEIRESMLSLLVKLHQKLSAKQNSLSLSWLA 1250

Query: 1601 QKSKLVSLIDRGPVSWDQPYWRDEHASTMSKRDLDQ-SGLSTSSGGSGVISSPQLAELIQ 1660
            +          G  + ++   +    S  SKR L +  G          IS      + +
Sbjct: 1251 EVDPAHHAHGDGLTAIERILAKASARSRHSKRCLQEICGKVCPPIPPKKISPGDKKSMDK 1310

Query: 1661 NAVKEYANHGLPGEVGAFLDFVKSHFPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSV 1720
               ++ A       +  F    KS      +++ P    V     + S ++L+ ++    
Sbjct: 1311 EERRQRARERQQKLLAEFASRQKSFMETAMDVESPEADAVMD---VSSEESLDSEV---- 1370

Query: 1721 RKEMHDIL----HSKLNEDEKFSEVL---SGLVSGEDCKSVLHVKYIAALSRELAENHPT 1780
               ++D +         ED     V+   +  V G  C+S +  K +     E      T
Sbjct: 1371 ---LYDCVICGQSGPSTEDRPTGLVVLLQASSVLGHRCRSDM-PKRLPTTDEEHIYPEDT 1430

Query: 1781 SESAHNVSMPIESLQPTTFNEIGPIDC------DGIYLSSCGHAVHQGCLDRYLSSLKER 1840
              + H+V +   SL    F +   +         G+Y+ +CGH +H  C   Y+ SL+  
Sbjct: 1431 CGATHDVRL---SLMQRYFKDSSCLQSVSIGWDGGVYVQTCGHTLHIDCHKSYMESLR-- 1490

Query: 1841 FARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAIPREFKK--IWN-PSMSSIGSLSRV 1900
                 V +G   +  ++GEF CP+CR+ ANS LP  P    +   W+ PS  S+ +L + 
Sbjct: 1491 --NDQVLQG---ISVDKGEFTCPLCRQFANSVLPCRPGRGMETGAWHAPSTKSMSTLVK- 1550

Query: 1901 SGCLNKSSEEVNPLYVQQAVALLQSAAKAVGKSKILKDISVHRHRKVSRNLEAVSLVLSK 1960
                     EV  L  Q  +  ++++ K   +  IL +        +S+ +E+V     K
Sbjct: 1551 ---------EVEDLQEQLGIFPVRASIKQ--RDPILIE------SNLSKEMESV----IK 1610

Query: 1961 LYFSGKQDKLISFTRVNPSILMWDTLKYSLISMETAAVELKTSGGFILSLLLKVIQSIKR 2020
               +  Q K + + + NP     D L    ++     +EL   GG   +L      +  +
Sbjct: 1611 DIKNTTQKKYMDYGK-NPGSPDNDFLFMYSVARTNLELELVHRGG---NLCSGGASAAAK 1670

Query: 2021 EDSLLLLQRFCGIQRYPSHLDKSSCGLPQFDIQFLSRGSD---------PVLAHDPFASL 2080
               L  L     +      +D +     +  +   SR ++         P+L  D  + L
Sbjct: 1671 RSCLNQLFHVLAMHMRLYSIDSAYNPWTRLTLSTQSRENEYCDEERPEVPMLFRDVPSLL 1730

Query: 2081 MWVFFCLPCPFLSCKESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIAD--- 2140
            +     +P P    KE    +V + Y +   QA+    I++  + + L +++   A    
Sbjct: 1731 IIFILTMPQPLR--KEHFTCVVKVLYSLQFTQALAALSIRFS-REERLAWSNTGAAKKNL 1790

Query: 2141 ----------ICKIMGESEYARRYFVSNYTEPSC---------NVKDMIRSLTFPYLR-R 2200
                      +  ++ E   A+  + +N  E S          +++  ++    P+LR  
Sbjct: 1791 PNSDKSWESLLGHMISELTKAKDVYDTNSEETSALSSSVWSPQSIEFRLQQFCLPFLRLS 1840

Query: 2201 CALLLKLLNSSARVPIFDGE-TAFERYLAVDDMIDNIALELNEIEKLEKMFEIPPLDIVL 2260
            C L   L   S    + + E +   R L +   +   +  ++    LE  + I   D++ 
Sbjct: 1851 CLLQHHLYGDSLPGCLVEEEFSLLTRCLGLAASV-QCSGSMSSAACLE--WNISAFDLIS 1840

Query: 2261 KDRASRSLVWKWFCHFNKEFEFQRSKNLKHCTP--AVPFQLIRLPHVYHDLLQRYIKKRC 2300
            +        W        +   Q+S +L    P  A P +L++LP  Y+ + Q Y +K C
Sbjct: 1911 Q--------WCSEVVALSDTPSQQSASLLGQDPQWAAP-RLLQLPDNYNTIFQYYHRKSC 1840

BLAST of Sgr022730 vs. ExPASy Swiss-Prot
Match: Q6ZT12 (E3 ubiquitin-protein ligase UBR3 OS=Homo sapiens OX=9606 GN=UBR3 PE=2 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-50
Identity = 443/2054 (21.57%), Postives = 756/2054 (36.81%), Query Frame = 0

Query: 364  LVSAILPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGG----EPTFAL 423
            L+  +L  +  +A       A +     GP    +  E++ W + L+ GG    E   A+
Sbjct: 54   LLERVLSAERPLAAAAGGEDAAAAGGGGGPGAAEE--EALEWCKCLLAGGGGYDEFCAAV 113

Query: 424  KNLSKMSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYT-G 483
            +     +     +CG VW  N +AYRCRTC   P  ++C  CF  G+H  HD+++  +  
Sbjct: 114  R-----AYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQA 173

Query: 484  GGCCDCGDVTAWKREGFCSKH--KGAEQIQPLPEEFVKSVGPILDA-LFTSWKNKLLSAE 543
            GG CDCGD    +  GFC +H  K +  I  +P++ +     +L   +F   +       
Sbjct: 174  GGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYN 233

Query: 544  DISVEDPKLSDRVTEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILV 603
            + + + P   D     + +  +++F     L D  K   ++ S +++ + +   + D+  
Sbjct: 234  EPAADGPSEKDLNKVLQLLEPQISF-----LEDLTKMGGAMRSVLTQVLTNQQNYKDLTS 293

Query: 604  RLERLLTDIVVKKVHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPL 663
             L     +  VKK HE  L  L      Y   K+            ++E S         
Sbjct: 294  GLGE---NACVKKSHEKYLIALKSSGLTYPEDKLVY---------GVQEPSAGT------ 353

Query: 664  LPTFSVQIFTVPTLTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIR 723
              + +VQ F   T T   V+  +         ED       +DG   + K   +  ++  
Sbjct: 354  -SSLAVQGFIGATGTLGQVDSSD---------ED------DQDGSQGLGKRKRVKLSSGT 413

Query: 724  VVEDIRFVMSHAVVPRYVIYEQQDILRTWLRLLTF--VQGMDPQKRETGLHIEEENENVH 783
              + I  V+ H               +++L  L F  ++   PQK  T L        ++
Sbjct: 414  KDQSIMDVLKH---------------KSFLEELLFWTIKYEFPQKMVTFL--------LN 473

Query: 784  LPFGLDHSVANIHSLLVNEAFSAATGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQ 843
            +    ++ VA   + + + AF   T      +D MS           + I H  V   S 
Sbjct: 474  MLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMS-----------NRIVHISVQLFSN 533

Query: 844  ESAACNVLGRSSTSTSASRIDEVCS--DAISSTIMWLTYECLKVIDSWLGTENTSGGI-- 903
            E               A ++ E C   D + + ++++   CL  I S L  E  S  +  
Sbjct: 534  EEL-------------ARQVTEECQLLDIMVTVLLYMMESCL--IKSELQDEENSLHVVV 593

Query: 904  ---PDMFAENTSMTSSCKLYSLRKTSALESKKMSSK---VEKGKFV--FE--KLARKSKN 963
                 +   NT         ++    ++  + +      V    FV  F+   L ++  N
Sbjct: 594  NCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELN 653

Query: 964  YNRQYSSRMY---SGLQMSIDHQHGITLGEDNQLMDVTNDTVTVEDYAMENDALHFLSLS 1023
             + ++ S+ Y      ++    Q    L    ++ +    T  V  Y +E       +L 
Sbjct: 654  EHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLE-------ALQ 713

Query: 1024 AW---PNIVYDVSSQDISIHIPLHRLLSLLLQKALRSCFSESVVPSSTGASSSNLSLEYV 1083
             W    N V + +   ++ H+PLHR  ++ L KA++                  L L+  
Sbjct: 714  DWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVK---------------CQELDLD-- 773

Query: 1084 DFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNTGMWRRNGDAALLSCELYRSIRWSEQ 1143
                SVL D         LM HPL+I+   AE+++ MW RNG         Y    +   
Sbjct: 774  ----SVLPD---QEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNS 833

Query: 1144 CLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYLSL-----NVERPSEYEPILVQEMLT 1203
             ++ D++LLQ CA+   PD ++S + ERF++ D L++     N    +E+E  +++  LT
Sbjct: 834  MIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALT 893

Query: 1204 LIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPRD-------LSKCHQLQ 1263
             ++ +++ R   G++  E L+ E++ +L + D THS L+  +P +       +   +  +
Sbjct: 894  FLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFE 953

Query: 1264 EILDTIAVYSNP-----SGFNQGMYSLSWKYW-KELD---LYHPRWSLRDLQIAEERY-- 1323
             +L  +A +  P         QGMY+   + W +E D   +       RD+Q A +RY  
Sbjct: 954  SVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDQEFDPVMVILRTVYRRDVQSAMDRYTA 1013

Query: 1324 -LRSCGVSALTSQLP--KWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRA 1383
             L+  G        P  K T ++P + GL R+  CK  L IV   L Y +  +    S  
Sbjct: 1014 FLKQSGKFPGNPWPPYKKRTSLHPSYKGLMRLLHCK-TLHIVLFTLLYKILMDHQNLS-- 1073

Query: 1384 PDSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLL 1443
             + VL   L+L+ L L+     +E SD+    G          E   +    G    S +
Sbjct: 1074 -EHVLCMVLYLIELGLE--NSAEEESDEEASVG--------GPERCHDSWFPGSNLVSNM 1133

Query: 1444 SLLILLMKMH----------------KKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRC 1503
               I  +++                   +   +L  +G+  + SLV    KKF EI +R 
Sbjct: 1134 RHFINYVRVRVPETAPEVKRDSPASTSSDNLGSLQNSGTAQVFSLVAERRKKFQEIINRS 1193

Query: 1504 MGKVQQLAPEIFGYLSQSVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLA 1563
              +  Q+         +   SS  ++            + +E   ++L K+  + S    
Sbjct: 1194 SSEANQVV--------RPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQN 1253

Query: 1564 SFDSSVNDDTEFWQESEKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLID 1623
            S+     DD E   + E P  S              D  ++V    L    +S++   I 
Sbjct: 1254 SYYPPWLDDIEILIQPEIPKYSHG------------DGITAVERILLKAASQSRMNKRII 1313

Query: 1624 RGPVSWDQPYWRDEHASTMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGL 1683
                    P    +  +   K+ LD+                +  E  Q  + E+A+   
Sbjct: 1314 EEICRKVTPPVPPKKVTAAEKKTLDKEE-----------RRQKARERQQKLLAEFASR-- 1373

Query: 1684 PGEVGAFLDFVKSHFPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSK 1743
                       KS      ++  P         I     T E  +  +V   +       
Sbjct: 1374 ----------QKSFMETAMDVDSPEND------IPMEITTAEPQVSEAVYDCVICGQSGP 1433

Query: 1744 LNEDEKFSEVL---SGLVSGEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQ 1803
             +ED     V+   +  V G+ C+  +  K +     E      T  + H+V +   SL 
Sbjct: 1434 SSEDRPTGLVVLLQASSVLGQ-CRDNVEPKKLPISEEEQIYPWDTCAAVHDVRL---SLL 1493

Query: 1804 PTTFNEIGPIDC------DGIYLSSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDP 1863
               F +   +         G+Y+ +CGH +H  C   Y+ SL+       V +G  +   
Sbjct: 1494 QRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLR----NDQVLQGFSV--- 1553

Query: 1864 EQGEFLCPVCRRLANSTLPAIPR---EFKKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLY 1923
            ++GEF CP+CR+ ANS LP  P    E      PS  SI  L +          EV  L 
Sbjct: 1554 DKGEFTCPLCRQFANSVLPCYPGSNVENNPWQRPSNKSIQDLIK----------EVEELQ 1613

Query: 1924 VQQAVALLQSAAKAVGKSKILKDISVHRHRKVSRNLEAVSLVLSKLYFSGKQDKLISFTR 1983
             +   A       +     ++KDI     +K            SK   S   D L  ++ 
Sbjct: 1614 GRPG-AFPSETNLSKEMESVMKDIKNTTQKKYRD--------YSKTPGSPDNDFLFMYSV 1673

Query: 1984 VNPSILMWDTLKYSLISMETAAVELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQR 2043
               +      L+  LI           S     S L ++   +     L  +       R
Sbjct: 1674 ARTN------LELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWR 1733

Query: 2044 YPSHLDKSSCGLPQFDIQFLSRGSDPVLAHDPFASLMWVFFCLPCPFLSCKESLLSLVHI 2103
              + L++ +   PQ   +   +   P+L HD  + L+     +P P    K+    +V +
Sbjct: 1734 KLTQLEEMN---PQLGYE-EQQPEVPILYHDVTSLLLIQILMMPQPLR--KDHFTCIVKV 1793

Query: 2104 FYLVSVAQAIITSCIKYQCKTDGLG---FNDCLIADICKIMGESEYARRYFVSN------ 2163
             + +   QA+    +K  C  +          L    C      E    + +S       
Sbjct: 1794 LFTLLYTQALAALSVK--CSEEDRSAWKHAGALKKSTCDAEKSYEVLLSFVISELFKGKL 1853

Query: 2164 YTEPSC--------------NVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFE 2223
            Y E                 +++  ++    P+LR  +LL   L     +P    E  F 
Sbjct: 1854 YHEEGTQECAMVNPIAWSPESMEKCLQDFCLPFLRITSLLQHHL-FGEDLPSCQEEEEFS 1865

Query: 2224 RYLAVDDMIDNI-ALELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQR 2283
               +   ++      E   I      + +P  DI+ +         +      K    Q 
Sbjct: 1914 VLASCLGLLPTFYQTEHPFISASCLDWPVPAFDIITQWCFEIKSFTERHAEQGKALLIQE 1865

Query: 2284 SK-NLKHCTPAVPFQLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWK 2300
            SK  L H        L++LP  Y+ + Q Y +K C  C  V  DPA+CL+CG        
Sbjct: 1974 SKWKLPH--------LLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGL 1865

BLAST of Sgr022730 vs. ExPASy Swiss-Prot
Match: Q5U430 (E3 ubiquitin-protein ligase UBR3 OS=Mus musculus OX=10090 GN=Ubr3 PE=1 SV=3)

HSP 1 Score: 193.4 bits (490), Expect = 3.0e-47
Identity = 415/1995 (20.80%), Postives = 730/1995 (36.59%), Query Frame = 0

Query: 432  VCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYT-GGGCCDCGDVTAW 491
            +CG VW  N +AYRCRTC   P  ++C  CF  G+H  HD+++  +  GG CDCGD    
Sbjct: 119  LCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVM 178

Query: 492  KREGFCSKH--KGAEQIQPLPEEFVKSVGPILDA-LFTSWKNKLLSAEDISVEDPKLSDR 551
            +  GFC +H  K +  I  +P++ +     +L   +F   +       + + + P   D 
Sbjct: 179  RESGFCRRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADAPSEKDL 238

Query: 552  VTEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVK 611
                + +  +++F     L D  K   ++ S +++ + +   + D+   L     +   K
Sbjct: 239  NKVLQLLEPQISF-----LEDLTKMGGAMRSVLTQVLTNQQNYKDLTAGLGE---NACAK 298

Query: 612  KVHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVP 671
            K HE  L  L      Y   K+           +                T + Q F   
Sbjct: 299  KSHEKYLIALKSSGLTYPEDKLVYGVQEPAAGTS----------------TLAAQGFAGA 358

Query: 672  TLTPRLV----EEMNLLSILLGCLEDIFISCVSED-GRLQVIKWSNLYETTIRVVEDIRF 731
            T T   +    EE    S  LG  + + +S  ++D   + V+K  +        +E++ F
Sbjct: 359  TGTLGQIDSSDEEDQDGSQGLGKRKRVKLSSGTKDQSIMDVLKHKSF-------LEELLF 418

Query: 732  VMSHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSV 791
                   P+              +++TF+  M P +                    ++ V
Sbjct: 419  WTIKYEFPQ--------------KMVTFLLNMLPDQ--------------------EYKV 478

Query: 792  ANIHSLLVNEAFSAATGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLG 851
            A   + + + AF   T      +D MS           + I H  V   S E        
Sbjct: 479  AFTKTFVQHYAFIMKTLKKSHESDTMS-----------NRIVHISVQLFSNEEL------ 538

Query: 852  RSSTSTSASRIDEVCS--DAISSTIMWLTYECLKVIDSWLGTENTSGGI-----PDMFAE 911
                   A ++ E C   D + + ++++   CL  I S L  E  S  +       +   
Sbjct: 539  -------ARQVTEECQLLDIMVTVLLYMMESCL--IKSELQDEENSLHVVVNCGEALLKN 598

Query: 912  NTSMTSSCKLYSLRKTSALESKKMSSK---VEKGKFV--FE--KLARKSKNYNRQYSSRM 971
            NT         ++    ++  + +      V    FV  F+   L ++  N + ++ S+ 
Sbjct: 599  NTYWPLVSDFINILSHQSVAQRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQT 658

Query: 972  Y---SGLQMSIDHQHGITLGEDNQLMDVTNDTVTVEDYAMENDALHFLSLSAW---PNIV 1031
            Y      ++    Q    L    ++ +    T  V  Y +E       +L  W    N V
Sbjct: 659  YYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLE-------ALQDWFDAINFV 718

Query: 1032 YDVSSQDISIHIPLHRLLSLLLQKALRSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTD 1091
             + +   ++ H+PLHR  ++ L KA++                  L L+      S+L D
Sbjct: 719  DEPAPNQVTFHLPLHRYYAMFLSKAVK---------------CQELDLD------SLLPD 778

Query: 1092 CHPYGFSAFLMEHPLRIKVFCAEVNTGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLL 1151
                     LM HPL+I+   AE+++ MW RNG         Y    +    ++ D++LL
Sbjct: 779  ---QEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLL 838

Query: 1152 QCCAAMAPPDLYVSRILERFRLSDYLSL-----NVERPSEYEPILVQEMLTLIIQVVNER 1211
            Q CA+   PD ++S + ERF++ D L++     N+    E+E  +++  LT ++ +++ R
Sbjct: 839  QVCASRLDPDYFISSVFERFKVVDLLTMASQHHNMVLDVEHERSMLEGALTFLVILLSLR 898

Query: 1212 RFCGLTVAESLKRELIYKLAIGDATHSQLVKALPRD-------LSKCHQLQEILDTIAVY 1271
               G++  + L+ E++ +L + D THS L+  +P +       +   +  + +L  +A +
Sbjct: 899  LHLGMSDDDILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADF 958

Query: 1272 SNP-----SGFNQGMYSLSWKYW-KELD---LYHPRWSLRDLQIAEERY---LRSCGVSA 1331
              P         QGMY+   + W +E D   +       RD+Q A +RY   L+  G   
Sbjct: 959  RAPIFEPGGSMQQGMYTPKAEVWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFP 1018

Query: 1332 LTSQLP--KWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAPDSVLLSAL 1391
                 P  K T ++P + GL R+  CK  L IV   L Y +  +    S   + VL   L
Sbjct: 1019 GNPWPPYKKRTSLHPSYKGLMRLLHCK-TLHIVLFTLLYKILMDHQNLS---EHVLCMVL 1078

Query: 1392 HLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFG------RQSLLSLL 1451
            +L+ L L+         + S    +      F    +   + +          ++   L 
Sbjct: 1079 YLIELGLENSADDDSEEEVSMGGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPELK 1138

Query: 1452 ILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLA-PEIFGYLSQS 1511
               +     +  ++L  +G+  + SLV    KKF EI +R   +  Q+  P+I      S
Sbjct: 1139 RDPLASTSSDALDSLQNSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKIPS--KWS 1198

Query: 1512 VPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSVNDDTEFWQESEK 1571
             P S     +          + +E   ++L K+  + S    S+     DD E   + E 
Sbjct: 1199 APGSSPQLTTAI-------LEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEVLIQPEI 1258

Query: 1572 PNVS-----------------DSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDR 1631
            P  +                  S      +  +C   +  VP   +   +K  L     R
Sbjct: 1259 PKYNHGDGITAVERILLKAAVQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERR 1318

Query: 1632 GPVSWDQPYWRDEHASTMSKRDLDQSGLSTSSGGSGV-----ISSPQLAELIQNAV---- 1691
                  Q     E AS   ++   ++ +   S  + +      + PQ++E + + V    
Sbjct: 1319 QKARERQQKLLAEFAS--RQKSFMETAMDVDSPENDIPMEITTAEPQVSEAVYDCVICGQ 1378

Query: 1692 KEYANHGLPGEVGAFLDFVKSHFPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKE 1751
               ++   P  +   L    S     R+   P    +  E+ I+ +DT            
Sbjct: 1379 SGPSSEDRPTGLVVLLQ-ASSVLGQCRDNAEPKKLPIAEEEQIYPWDTC---------AA 1438

Query: 1752 MHDILHSKLNEDEKFSEVLSGLVSGEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMP 1811
            +HD+  S L    K S  L  +  G +                                 
Sbjct: 1439 VHDVRLSLLQRYFKDSSCLLAVSIGWE--------------------------------- 1498

Query: 1812 IESLQPTTFNEIGPIDCDGIYLSSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPE 1871
                              G+Y+ +CGH +H  C   Y+ SL+       V +G  +   +
Sbjct: 1499 -----------------GGVYVQTCGHTLHIDCHKSYMESLR----NDQVLQGFSV---D 1558

Query: 1872 QGEFLCPVCRRLANSTLPAIPREFKKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQA 1931
            +GEF CP+CR+ ANS LP  P           S++ +      C NKS+++         
Sbjct: 1559 KGEFTCPLCRQFANSVLPCYPG----------SNVENNLWQRPC-NKSTQD--------- 1618

Query: 1932 VALLQSAAKAVGKSKILKDISVHRHRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPS 1991
              L++   +  G+              +S+ +E+V     K   +  Q K   +++   S
Sbjct: 1619 --LIKEVEELQGRPGAFPS-----ETNLSKEMESV----MKDIKNTTQKKYRDYSKTPGS 1678

Query: 1992 ILMWDTLKYSLISMETAAVELKTSGGFIL----SLLLKVIQSIKREDSLLLLQRFCGIQR 2051
                    YS ++     +EL   GG +     S   K     +    L L  R   I  
Sbjct: 1679 PDNEFLFMYS-VARTNLELELIHRGGSLCSGGPSTAGKRSCLNQLFHVLALHMRLYTIDS 1738

Query: 2052 YPSHLDKSSCGLPQFDIQFLSRGSD---PVLAHDPFASLMWVFFCLPCPFLSCKESLLSL 2111
              +   K +  +   + Q  +       P+L HD  + L+     +P P    KE    +
Sbjct: 1739 EYNPWKKLTQLVEDMNSQVGNEDQQPEVPILYHDVTSLLLIQILMMPQPLR--KEHFTCI 1798

Query: 2112 VHIFYLVSVAQAIITSCIK------------YQCKTDGLGFNDCLIADICKIMGESEYAR 2171
            V + + +   QA+    +K               + D      C    +  ++ E    +
Sbjct: 1799 VKVLFTLLYTQALAALSVKGTEEDRSAWKHAGALRKDTCDAEKCYEVLLSFVISELSKGK 1858

Query: 2172 RYFVSNYTEPSC---------NVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAF 2231
             Y+     E +          +++  I+    P+LR  +LL   L     +P    E  F
Sbjct: 1859 LYYEEGAQECAMVSPIAWSPESMERYIQDFCLPFLRVSSLLQHHL-FGEDLPSCQEEEEF 1866

Query: 2232 ERYLAVDDMIDNIALELNEIEKLEKM-FEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQ 2291
                +   ++       +       + + +   DI+ +     +   +      K    Q
Sbjct: 1919 SVLASCLGLLPTFYQTDHPFISASCLDWPVAAFDIITQWCFEITSFTERHAEQGKALLIQ 1866

Query: 2292 RSK-NLKHCTPAVPFQLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSW 2300
             S+  L H        L++LP  Y+ + Q Y +K C  C  V  DPA+CL+CG       
Sbjct: 1979 ESRWKLPH--------LLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKG 1866

BLAST of Sgr022730 vs. ExPASy Swiss-Prot
Match: O80931 (Transcription factor AS1 OS=Arabidopsis thaliana OX=3702 GN=AS1 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.8e-39
Identity = 86/114 (75.44%), Postives = 100/114 (87.72%), Query Frame = 0

Query: 164 FCFELVECCRELDEGHRAWAAHKKEAAWRLRRLELQLESEKASRIRQKMEEVEGKVKALR 223
           F  ELVECCREL+EGHRAWA HKKEAAWRLRRLELQLESEK  R R+KMEE+E K+KALR
Sbjct: 250 FLSELVECCRELEEGHRAWADHKKEAAWRLRRLELQLESEKTCRQREKMEEIEAKMKALR 309

Query: 224 EEEKIALERIEAEYKEQLAGLRKDAEAKEQKLAEQWAAKHLSLTKFLE-HMGCR 277
           EE+K A+E+IE EY+EQL GLR+DAEAK+QKLA+QW ++H+ LTKFLE  MGCR
Sbjct: 310 EEQKNAMEKIEGEYREQLVGLRRDAEAKDQKLADQWTSRHIRLTKFLEQQMGCR 363

BLAST of Sgr022730 vs. ExPASy TrEMBL
Match: A0A6J1CUV9 (E3 ubiquitin-protein ligase OS=Momordica charantia OX=3673 GN=LOC111014998 PE=3 SV=1)

HSP 1 Score: 3599.7 bits (9333), Expect = 0.0e+00
Identity = 1825/2040 (89.46%), Postives = 1898/2040 (93.04%), Query Frame = 0

Query: 309  MDDMDIGSPSESVPLKPRDRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAI 368
            MDDM+IGSPSES PLKPRDRIVRRLA+LGVPE+LLDQLQPGLVSFVKDNK LI +LVSAI
Sbjct: 1    MDDMNIGSPSESAPLKPRDRIVRRLALLGVPEELLDQLQPGLVSFVKDNKILIPQLVSAI 60

Query: 369  LPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVG 428
            LPTDVEVAEVIRDAKAGSKKSV GPTMKNQFRESM+WLQWLMF GEPTFALKNLSKMS G
Sbjct: 61   LPTDVEVAEVIRDAKAGSKKSVAGPTMKNQFRESMVWLQWLMFEGEPTFALKNLSKMSFG 120

Query: 429  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 488
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 180

Query: 489  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 548
            AWKREGFCSKHKGAE+IQPLPEEFV+SVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAERIQPLPEEFVESVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 549  TEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKK 608
             EHKK+ANELTFAVVEMLLDFCKYSESLLSFVSKRVIS AGFLDILVRLERLLTDIVVKK
Sbjct: 241  MEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISLAGFLDILVRLERLLTDIVVKK 300

Query: 609  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPT 668
            VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHA KKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHALKKYPLLPTFSVQIFTVPT 360

Query: 669  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 728
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 420

Query: 729  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 788
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIH+L
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHTL 480

Query: 789  LVNEAFSAA--TGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSST 848
            LVNEAFSAA  + S+EETADAMSF  +KQNP+D+DSIRHAKVGRLSQESAACNV GRSST
Sbjct: 481  LVNEAFSAASSSSSSEETADAMSFHAFKQNPDDMDSIRHAKVGRLSQESAACNVFGRSST 540

Query: 849  STSASRIDEVCSDAISSTIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLY 908
             TSASR+DEVCSD ISSTIMWLTYECLKVIDSWLGTEN  GG P+MFAEN SM SSCK+Y
Sbjct: 541  VTSASRVDEVCSDTISSTIMWLTYECLKVIDSWLGTENALGGFPNMFAENASMASSCKVY 600

Query: 909  SLRKTSALESKKMSSKVEKGKFVFEKLARKSKNYNRQYSSRMYSGLQMSIDHQHGITLGE 968
            SLRK SALES+K+SSKVEKGKF+FEK+A KSK++NRQYSSR+YSGLQMSID++HG++LGE
Sbjct: 601  SLRKPSALESRKISSKVEKGKFIFEKVAGKSKDHNRQYSSRIYSGLQMSIDNEHGVSLGE 660

Query: 969  DNQLMDVTNDTVTVEDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 1028
            DNQLMDVTNDTVTVEDYAME DALHFLS SAWPNIVYDVSSQDISIHIPLHRLLSLLLQK
Sbjct: 661  DNQLMDVTNDTVTVEDYAMETDALHFLSFSAWPNIVYDVSSQDISIHIPLHRLLSLLLQK 720

Query: 1029 ALRSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEV 1088
            ALRS FSESVVPS+TGASSSNLS EYVDFFKSVLTD HPYGFSAF+MEHPLRIKVFCAEV
Sbjct: 721  ALRSSFSESVVPSATGASSSNLSFEYVDFFKSVLTDYHPYGFSAFVMEHPLRIKVFCAEV 780

Query: 1089 NTGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSD 1148
            N GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPP+LY+SRILERFRL D
Sbjct: 781  NAGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPELYISRILERFRLLD 840

Query: 1149 YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 1208
            YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL
Sbjct: 841  YLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQL 900

Query: 1209 VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIA 1268
            VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQIA
Sbjct: 901  VKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLRWKYWKELDLYHPRWSLRDLQIA 960

Query: 1269 EERYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSR 1328
            EERYLRSCG SALTSQLPKWTKIYPPF GLARIATCK VLQI+RAVLFYSVFT+KSTKSR
Sbjct: 961  EERYLRSCGASALTSQLPKWTKIYPPFRGLARIATCKAVLQIIRAVLFYSVFTDKSTKSR 1020

Query: 1329 APDSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSL 1388
            APDSVLL+ALHLLALALDICFQ+KE SDQSFCS DSIPLLLFADEEVDEGLTYG GRQSL
Sbjct: 1021 APDSVLLTALHLLALALDICFQRKESSDQSFCSEDSIPLLLFADEEVDEGLTYGLGRQSL 1080

Query: 1389 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYL 1448
            LSLLILLMKMHKKEGRENLLEAGSCNLSSLVE LLKKFSEIDSRCMGK++Q APEI GYL
Sbjct: 1081 LSLLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSRCMGKIRQHAPEILGYL 1140

Query: 1449 SQSVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSVNDDTEFWQE 1508
            SQS+PSSD NSLS TSDS+K KAKARERQAAILEKMRAEQ KFLAS DSSV DD EF QE
Sbjct: 1141 SQSLPSSDPNSLSETSDSDKLKAKARERQAAILEKMRAEQYKFLASVDSSVEDDAEFGQE 1200

Query: 1509 SEKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEH 1568
            SEKP+VSDSAEQSETVCSLCHDSSSSVPIS+LILLQKSKLVSLIDRG VSWDQPYWRDEH
Sbjct: 1201 SEKPSVSDSAEQSETVCSLCHDSSSSVPISYLILLQKSKLVSLIDRGTVSWDQPYWRDEH 1260

Query: 1569 ASTMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSHF 1628
            AS++ +R+LDQS LSTSSGG   ISSPQLA+LIQNAVKEYA+HGLPGEVGAFLDFVKSHF
Sbjct: 1261 ASSIPRRNLDQSELSTSSGG---ISSPQLAQLIQNAVKEYASHGLPGEVGAFLDFVKSHF 1320

Query: 1629 PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKLNEDEKFSEVLSGLV 1688
            PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMH+ +HSKLNEDE  S+V S LV
Sbjct: 1321 PPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHENMHSKLNEDENLSKVASSLV 1380

Query: 1689 SGEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPIDCDGIYLS 1748
            + ED KSVLHVKYIAALSRELAENH TSESAHNVSMP+ESLQPTTFNEI P+DCDGIYLS
Sbjct: 1381 T-EDSKSVLHVKYIAALSRELAENHSTSESAHNVSMPVESLQPTTFNEIDPVDCDGIYLS 1440

Query: 1749 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAIPRE 1808
            SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPA PRE
Sbjct: 1441 SCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAFPRE 1500

Query: 1809 FKKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQAVALLQSAAKAVGKSKILKDISVH 1868
            F+K  NPSMSSIGSLS VSG  NKS+EEVNPLYVQ+AVA LQS AKAVGK+K+LKDISVH
Sbjct: 1501 FQKFGNPSMSSIGSLSHVSGQSNKSTEEVNPLYVQKAVAFLQSTAKAVGKNKVLKDISVH 1560

Query: 1869 RHRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPSILMWDTLKYSLISMETAA----- 1928
            RHRKVSRNLEAVSLVLSKLYFSGKQDK IS TRVNPSILMWDTLKYSL+SME AA     
Sbjct: 1561 RHRKVSRNLEAVSLVLSKLYFSGKQDKSISCTRVNPSILMWDTLKYSLVSMEIAARSKTD 1620

Query: 1929 -----------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQRYPSHL------ 1988
                        ELKTSGGFILSLLLKVIQSIK EDSLLLLQRFCGIQR+   +      
Sbjct: 1621 VNPNIGLNTLYKELKTSGGFILSLLLKVIQSIKCEDSLLLLQRFCGIQRFADSICSGVSN 1680

Query: 1989 --DKSSCG-------------LPQFDIQFLSRGSDPVLAHDPFASLMWVFFCLPCPFLSC 2048
                 SCG             LPQFDIQFLSRGSDPVLAHDPFAS+MWV FCLPCPFLSC
Sbjct: 1681 ENANDSCGQGILHILTSLRVELPQFDIQFLSRGSDPVLAHDPFASVMWVLFCLPCPFLSC 1740

Query: 2049 KESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGESEYARRYFVS 2108
            KESL+SLVHIFYLVSVAQ II SC+KYQ K DG+GF+DCLIADICKIMGESEYARRYFVS
Sbjct: 1741 KESLISLVHIFYLVSVAQGIIASCMKYQSKIDGMGFSDCLIADICKIMGESEYARRYFVS 1800

Query: 2109 NYTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLAVDDMIDNIA 2168
            +YTEPSCNVKDMIRSLTF YLRRCALLLKLLNSSAR PIFDGETA ERYLAVDDMIDNIA
Sbjct: 1801 DYTEPSCNVKDMIRSLTFTYLRRCALLLKLLNSSARAPIFDGETALERYLAVDDMIDNIA 1860

Query: 2169 LELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQRSKNLKHCTPAVPFQ 2228
            LELNEI KLEK+F+IPPLD+VLKD ASRSLVWKWFCHFN+EFEFQRSKNLKHCTPAVPFQ
Sbjct: 1861 LELNEIGKLEKIFKIPPLDVVLKDEASRSLVWKWFCHFNREFEFQRSKNLKHCTPAVPFQ 1920

Query: 2229 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 2288
            LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC
Sbjct: 1921 LIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANIC 1980

Query: 2289 AAGT-------EDHNLFQRSARQAPWPSPYLDAFGEEDLEMRRGKPLYLNEERYAALSYM 2303
            AAGT           L QRSARQAPWPSPYLDAFGEED+EMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2036

BLAST of Sgr022730 vs. ExPASy TrEMBL
Match: A0A1S3B5X9 (E3 ubiquitin-protein ligase OS=Cucumis melo OX=3656 GN=LOC103486385 PE=3 SV=1)

HSP 1 Score: 3482.2 bits (9028), Expect = 0.0e+00
Identity = 1762/2039 (86.41%), Postives = 1854/2039 (90.93%), Query Frame = 0

Query: 309  MDDMDIGSPSESVPLKPRDRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAI 368
            MDDMDIGSPSES PLKPRDRI+RRLAVLGVP++LLDQL  GLV FVKDNKFLI +LVSAI
Sbjct: 1    MDDMDIGSPSESTPLKPRDRILRRLAVLGVPDELLDQLFRGLVIFVKDNKFLIPQLVSAI 60

Query: 369  LPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVG 428
            LPTDVEV EVIRDA  GSKK++TGPTMK  FRESMMWLQWLMF GEP +ALKNLSKMSVG
Sbjct: 61   LPTDVEVVEVIRDAIPGSKKTLTGPTMKAHFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 429  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 488
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 489  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 548
            AWKREGFCSKHKGAEQIQPLPEE+VKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 549  TEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKK 608
            TEHKK+ANELTFAVVEMLLDFCK+SESLLSFVSKRVISSAG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 609  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPT 668
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHA KKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 669  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 728
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 729  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 788
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 789  LVNEAFSAATGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSSTST 848
            LV EAFSAA+ S E+TAD M FQTYKQN +DIDS+RHAKVGRLSQESAAC VLG+SSTST
Sbjct: 481  LVKEAFSAASSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVLGKSSTST 540

Query: 849  SASRIDEVCSDAISSTIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLYSL 908
            SASR+DEVCSDAISSTIMWLTYECLK+ID+WLGTEN SG IP+M  E+ S   SCK YS 
Sbjct: 541  SASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAPSCKFYSS 600

Query: 909  RKTSALESKKMSSKVEKGKFVFEKLARKSKNYNRQYSSRMYSGLQMSIDHQHGITLGEDN 968
            RKTSAL SKKMS K+EKGKF+F+KL+R+SK +NRQYSSRMYSGLQMSID++HGI+LGEDN
Sbjct: 601  RKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGEDN 660

Query: 969  QLMDVTNDTVTVEDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 1028
             LMDVTNDTVT EDYAME DALHFLSLS+WPNIVYDVSSQDISIHIPLHRLLSLLLQKAL
Sbjct: 661  HLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 720

Query: 1029 RSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNT 1088
             SCFSES VPS+TGASSSNLS EYVDFFKSVLTDCHPYGFS+F+MEHPLRIKVFCAEVN 
Sbjct: 721  SSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEVNA 780

Query: 1089 GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYL 1148
            GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYV RILERFRLSDYL
Sbjct: 781  GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCRILERFRLSDYL 840

Query: 1149 SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVK 1208
            SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQLVK
Sbjct: 841  SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQLVK 900

Query: 1209 ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAEE 1268
            ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+AEE
Sbjct: 901  ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVAEE 960

Query: 1269 RYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAP 1328
            RYLRSCGVSALTSQLPKWTKIYPPF GLARIATCK VLQ +RAVLFYSVF E STKSRAP
Sbjct: 961  RYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSRAP 1020

Query: 1329 DSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLLS 1388
            DSVLLSALHLLALALD+CFQQKE  DQSF + DSIPLLLFA EE++EGL YGFGRQSLLS
Sbjct: 1021 DSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGLAYGFGRQSLLS 1080

Query: 1389 LLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYLSQ 1448
            LLILLMKMHKKEGRENLLEAGSCNLSSLVE LLKKFSEIDS CMGKVQQLAPEI GYLSQ
Sbjct: 1081 LLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYLSQ 1140

Query: 1449 SVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSV-NDDTEFWQES 1508
            SVP+S T+  + TSDSEKRKAKARERQAAILEKMRAEQSKFLAS D+SV +DDTEF QE 
Sbjct: 1141 SVPTSTTSRPAETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQEP 1200

Query: 1509 EKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1568
            EKPNVSD AEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA
Sbjct: 1201 EKPNVSDDAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1260

Query: 1569 STMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSHFP 1628
            ST SKRD+DQSG+STSS GS VISSPQLAELIQNAVKEY NHGLPGEVGAFLDF+KSHFP
Sbjct: 1261 STTSKRDIDQSGVSTSSAGSVVISSPQLAELIQNAVKEYTNHGLPGEVGAFLDFLKSHFP 1320

Query: 1629 PLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKLNEDEKFSEVLSGLVS 1688
            PLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMHD LHSK +E EK S V     S
Sbjct: 1321 PLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVFKEMHDTLHSKFHEGEKISTV----AS 1380

Query: 1689 GEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPIDCDGIYLSS 1748
            GED +SVLHVKYIAALSRELAEN  TSESA NV MP+ES QPT  NEIGPIDCDGIYLSS
Sbjct: 1381 GEDSRSVLHVKYIAALSRELAENRSTSESARNVHMPVESSQPTILNEIGPIDCDGIYLSS 1440

Query: 1749 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAIPREF 1808
            CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPA PREF
Sbjct: 1441 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPREF 1500

Query: 1809 KKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQAVALLQSAAKAVGKSKILKDISVHR 1868
            +KIWNP  SS+G+LS VSG LNKS+E VNPLY+Q+AVALLQSAAKAVGK+ +LKDISVHR
Sbjct: 1501 QKIWNPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISVHR 1560

Query: 1869 HRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPSILMWDTLKYSLISMETAA------ 1928
            H+KVSRNLEAVSLVLSKLYFS KQDKLIS +RVNPSILMWDTLKYSL+SME AA      
Sbjct: 1561 HKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKTDM 1620

Query: 1929 ----------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQRYPSHL------- 1988
                       ELKTSGGF+LSLLLKVI S+K EDSLLLLQR CGIQR+   +       
Sbjct: 1621 NPSIGLNTLYKELKTSGGFVLSLLLKVIHSVKCEDSLLLLQRLCGIQRFADSICSGMSNE 1680

Query: 1989 -DKSSCG-------------LPQFDIQFLSRGSDPVLAHDPFASLMWVFFCLPCPFLSCK 2048
                SCG             LP FD QFLSRGSDPV+AHDPFASLMWV FCLP PFLSC+
Sbjct: 1681 NASDSCGRGILHILTSLRSELPHFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLSCR 1740

Query: 2049 ESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGESEYARRYFVSN 2108
            ESLLSLVHIFYLVSV QAIIT   K QCK DGLG +DCLI DICKIMGESEYAR+YFVSN
Sbjct: 1741 ESLLSLVHIFYLVSVTQAIITYFTKSQCKVDGLGSSDCLITDICKIMGESEYARQYFVSN 1800

Query: 2109 YTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLAVDDMIDNIAL 2168
            YTEPSCN+KD +RSLTFP+LRRCALLL+LL+SSARVPIFDGETA E YL  ++MIDNI +
Sbjct: 1801 YTEPSCNIKDTVRSLTFPFLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNITV 1860

Query: 2169 ELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQRSKNLKHCTPAVPFQL 2228
            ELNE+EKL+KMF+IPPLDIVLKD  SR LV KWFCHFNKEFEFQRSK +KHCTPAVPFQL
Sbjct: 1861 ELNEVEKLQKMFDIPPLDIVLKDGTSRLLVSKWFCHFNKEFEFQRSKIIKHCTPAVPFQL 1920

Query: 2229 IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA 2288
            IRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANICA
Sbjct: 1921 IRLPHVYHDLLQRYIKKRCTDCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANICA 1980

Query: 2289 AGT-------EDHNLFQRSARQAPWPSPYLDAFGEEDLEMRRGKPLYLNEERYAALSYM 2303
            AGT           L QRSARQAPWPSPYLDAFGEED+EMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2035

BLAST of Sgr022730 vs. ExPASy TrEMBL
Match: A0A5A7TL84 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004420 PE=3 SV=1)

HSP 1 Score: 3482.2 bits (9028), Expect = 0.0e+00
Identity = 1762/2039 (86.41%), Postives = 1854/2039 (90.93%), Query Frame = 0

Query: 309  MDDMDIGSPSESVPLKPRDRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAI 368
            MDDMDIGSPSES PLKPRDRI+RRLAVLGVP++LLDQL  GLV FVKDNKFLI +LVSAI
Sbjct: 1    MDDMDIGSPSESTPLKPRDRILRRLAVLGVPDELLDQLFRGLVIFVKDNKFLIPQLVSAI 60

Query: 369  LPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVG 428
            LPTDVEV EVIRDA  GSKK++TGPTMK  FRESMMWLQWLMF GEP +ALKNLSKMSVG
Sbjct: 61   LPTDVEVVEVIRDAIPGSKKTLTGPTMKAHFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 429  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 488
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 489  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 548
            AWKREGFCSKHKGAEQIQPLPEE+VKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 549  TEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKK 608
            TEHKK+ANELTFAVVEMLLDFCK+SESLLSFVSKRVISSAG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 609  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPT 668
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHA KKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 669  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 728
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 729  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 788
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 789  LVNEAFSAATGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSSTST 848
            LV EAFSAA+ S E+TAD M FQTYKQN +DIDS+RHAKVGRLSQESAAC VLG+SSTST
Sbjct: 481  LVKEAFSAASSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVLGKSSTST 540

Query: 849  SASRIDEVCSDAISSTIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLYSL 908
            SASR+DEVCSDAISSTIMWLTYECLK+ID+WLGTEN SG IP+M  E+ S   SCK YS 
Sbjct: 541  SASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAPSCKFYSS 600

Query: 909  RKTSALESKKMSSKVEKGKFVFEKLARKSKNYNRQYSSRMYSGLQMSIDHQHGITLGEDN 968
            RKTSAL SKKMS K+EKGKF+F+KL+R+SK +NRQYSSRMYSGLQMSID++HGI+LGEDN
Sbjct: 601  RKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGEDN 660

Query: 969  QLMDVTNDTVTVEDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 1028
             LMDVTNDTVT EDYAME DALHFLSLS+WPNIVYDVSSQDISIHIPLHRLLSLLLQKAL
Sbjct: 661  HLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 720

Query: 1029 RSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNT 1088
             SCFSES VPS+TGASSSNLS EYVDFFKSVLTDCHPYGFS+F+MEHPLRIKVFCAEVN 
Sbjct: 721  SSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEVNA 780

Query: 1089 GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYL 1148
            GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYV RILERFRLSDYL
Sbjct: 781  GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCRILERFRLSDYL 840

Query: 1149 SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVK 1208
            SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQLVK
Sbjct: 841  SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQLVK 900

Query: 1209 ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAEE 1268
            ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+AEE
Sbjct: 901  ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVAEE 960

Query: 1269 RYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAP 1328
            RYLRSCGVSALTSQLPKWTKIYPPF GLARIATCK VLQ +RAVLFYSVF E STKSRAP
Sbjct: 961  RYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSRAP 1020

Query: 1329 DSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLLS 1388
            DSVLLSALHLLALALD+CFQQKE  DQSF + DSIPLLLFA EE++EGL YGFGRQSLLS
Sbjct: 1021 DSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGLAYGFGRQSLLS 1080

Query: 1389 LLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYLSQ 1448
            LLILLMKMHKKEGRENLLEAGSCNLSSLVE LLKKFSEIDS CMGKVQQLAPEI GYLSQ
Sbjct: 1081 LLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYLSQ 1140

Query: 1449 SVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSV-NDDTEFWQES 1508
            SVP+S T+  + TSDSEKRKAKARERQAAILEKMRAEQSKFLAS D+SV +DDTEF QE 
Sbjct: 1141 SVPTSTTSRPAETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQEP 1200

Query: 1509 EKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1568
            EKPNVSD AEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA
Sbjct: 1201 EKPNVSDDAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1260

Query: 1569 STMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSHFP 1628
            ST SKRD+DQSG+STSS GS VISSPQLAELIQNAVKEY NHGLPGEVGAFLDF+KSHFP
Sbjct: 1261 STTSKRDIDQSGVSTSSAGSVVISSPQLAELIQNAVKEYTNHGLPGEVGAFLDFLKSHFP 1320

Query: 1629 PLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKLNEDEKFSEVLSGLVS 1688
            PLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMHD LHSK +E EK S V     S
Sbjct: 1321 PLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVFKEMHDTLHSKFHEGEKISTV----AS 1380

Query: 1689 GEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPIDCDGIYLSS 1748
            GED +SVLHVKYIAALSRELAEN  TSESA NV MP+ES QPT  NEIGPIDCDGIYLSS
Sbjct: 1381 GEDSRSVLHVKYIAALSRELAENRSTSESARNVHMPVESSQPTILNEIGPIDCDGIYLSS 1440

Query: 1749 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAIPREF 1808
            CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPA PREF
Sbjct: 1441 CGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPREF 1500

Query: 1809 KKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQAVALLQSAAKAVGKSKILKDISVHR 1868
            +KIWNP  SS+G+LS VSG LNKS+E VNPLY+Q+AVALLQSAAKAVGK+ +LKDISVHR
Sbjct: 1501 QKIWNPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISVHR 1560

Query: 1869 HRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPSILMWDTLKYSLISMETAA------ 1928
            H+KVSRNLEAVSLVLSKLYFS KQDKLIS +RVNPSILMWDTLKYSL+SME AA      
Sbjct: 1561 HKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKTDM 1620

Query: 1929 ----------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQRYPSHL------- 1988
                       ELKTSGGF+LSLLLKVI S+K EDSLLLLQR CGIQR+   +       
Sbjct: 1621 NPSIGLNTLYKELKTSGGFVLSLLLKVIHSVKCEDSLLLLQRLCGIQRFADSICSGMSNE 1680

Query: 1989 -DKSSCG-------------LPQFDIQFLSRGSDPVLAHDPFASLMWVFFCLPCPFLSCK 2048
                SCG             LP FD QFLSRGSDPV+AHDPFASLMWV FCLP PFLSC+
Sbjct: 1681 NASDSCGRGILHILTSLRSELPHFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLSCR 1740

Query: 2049 ESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGESEYARRYFVSN 2108
            ESLLSLVHIFYLVSV QAIIT   K QCK DGLG +DCLI DICKIMGESEYAR+YFVSN
Sbjct: 1741 ESLLSLVHIFYLVSVTQAIITYFTKSQCKVDGLGSSDCLITDICKIMGESEYARQYFVSN 1800

Query: 2109 YTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLAVDDMIDNIAL 2168
            YTEPSCN+KD +RSLTFP+LRRCALLL+LL+SSARVPIFDGETA E YL  ++MIDNI +
Sbjct: 1801 YTEPSCNIKDTVRSLTFPFLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNITV 1860

Query: 2169 ELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQRSKNLKHCTPAVPFQL 2228
            ELNE+EKL+KMF+IPPLDIVLKD  SR LV KWFCHFNKEFEFQRSK +KHCTPAVPFQL
Sbjct: 1861 ELNEVEKLQKMFDIPPLDIVLKDGTSRLLVSKWFCHFNKEFEFQRSKIIKHCTPAVPFQL 1920

Query: 2229 IRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANICA 2288
            IRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANICA
Sbjct: 1921 IRLPHVYHDLLQRYIKKRCTDCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANICA 1980

Query: 2289 AGT-------EDHNLFQRSARQAPWPSPYLDAFGEEDLEMRRGKPLYLNEERYAALSYM 2303
            AGT           L QRSARQAPWPSPYLDAFGEED+EMRRGKPLYLNEERYAALSYM
Sbjct: 1981 AGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSYM 2035

BLAST of Sgr022730 vs. ExPASy TrEMBL
Match: A0A5D3DN55 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001660 PE=3 SV=1)

HSP 1 Score: 3466.4 bits (8987), Expect = 0.0e+00
Identity = 1761/2063 (85.36%), Postives = 1853/2063 (89.82%), Query Frame = 0

Query: 309  MDDMDIGSPSESVPLKPRDRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAI 368
            MDDMDIGSPSES PLKPRDRI+RRLAVLGVP++LLDQL  GLV FVKDNKFLI +LVSAI
Sbjct: 1    MDDMDIGSPSESTPLKPRDRILRRLAVLGVPDELLDQLFRGLVIFVKDNKFLIPQLVSAI 60

Query: 369  LPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVG 428
            LPTDVEV EVIRDA  GSKK++TGPTMK  FRESMMWLQWLMF GEP +ALKNLSKMSVG
Sbjct: 61   LPTDVEVVEVIRDAIPGSKKTLTGPTMKAHFRESMMWLQWLMFEGEPAYALKNLSKMSVG 120

Query: 429  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 488
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 489  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 548
            AWKREGFCSKHKGAEQIQPLPEE+VKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 549  TEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKK 608
            TEHKK+ANELTFAVVEMLLDFCK+SESLLSFVSKRVISSAG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDSVVKK 300

Query: 609  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPT 668
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHA KKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 669  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 728
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 729  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 788
            VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL
Sbjct: 421  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 789  LVNEAFSAATGSTEETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSSTST 848
            LV EAFSAA+ S E+TAD M FQTYKQN +DIDS+RHAKVGRLSQESAAC VLG+SSTST
Sbjct: 481  LVKEAFSAASSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVLGKSSTST 540

Query: 849  SASRIDEVCSDAISSTIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLYSL 908
            SASR+DEVCSDAISSTIMWLTYECLK+ID+WLGTEN SG IP+M  E+ S   SCK YS 
Sbjct: 541  SASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAPSCKFYSS 600

Query: 909  RKTSALESKKMSSKVEKGKFVFEKLARKSKNYNRQYSSRMYSGLQMSIDHQHGITLGEDN 968
            RKTSAL SKKMS K+EKGKF+F+KL+R+SK +NRQYSSRMYSGLQMSID++HGI+LGEDN
Sbjct: 601  RKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGEDN 660

Query: 969  QLMDVTNDTVTVEDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 1028
             LMDVTNDTVT EDYAME DALHFLSLS+WPNIVYDVSSQDISIHIPLHRLLSLLLQKAL
Sbjct: 661  HLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 720

Query: 1029 RSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNT 1088
             SCFSES VPS+TGASSSNLS EYVDFFKSVLTDCHPYGFS+F+MEHPLRIKVFCAEVN 
Sbjct: 721  SSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKVFCAEVNA 780

Query: 1089 GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYL 1148
            GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYV RILERFRLSDYL
Sbjct: 781  GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCRILERFRLSDYL 840

Query: 1149 SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVK 1208
            SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLT+AESLKRELIYKLAIGDATHSQLVK
Sbjct: 841  SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLAIGDATHSQLVK 900

Query: 1209 ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAEE 1268
            ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+AEE
Sbjct: 901  ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVAEE 960

Query: 1269 RYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAP 1328
            RYLRSCGVSALTSQLPKWTKIYPPF GLARIATCK VLQ +RAVLFYSVF E STKSRAP
Sbjct: 961  RYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSVFAEISTKSRAP 1020

Query: 1329 DSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLLS 1388
            DSVLLSALHLLALALD+CFQQKE  DQSF + DSIPLLLFA EE++EGL YGFGRQSLLS
Sbjct: 1021 DSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGLAYGFGRQSLLS 1080

Query: 1389 LLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYLSQ 1448
            LLILLMKMHKKEGRENLLEAGSCNLSSLVE LLKKFSEIDS CMGKVQQLAPEI GYLSQ
Sbjct: 1081 LLILLMKMHKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYLSQ 1140

Query: 1449 SVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSV-NDDTEFWQES 1508
            SVP+S T+  + TSDSEKRKAKARERQAAILEKMRAEQSKFLAS D+SV +DDTEF QE 
Sbjct: 1141 SVPTSTTSRPAETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQEP 1200

Query: 1509 EKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1568
            EKPNVSD AEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA
Sbjct: 1201 EKPNVSDDAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDEHA 1260

Query: 1569 STMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSHFP 1628
            ST SKRD+DQSG+STSS GS VISSPQLAELIQNAVKEY NHGLPGEVGAFLDF+KSHFP
Sbjct: 1261 STTSKRDIDQSGVSTSSAGSVVISSPQLAELIQNAVKEYTNHGLPGEVGAFLDFLKSHFP 1320

Query: 1629 PLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKLNEDEKFSEVLSGLVS 1688
            PLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMHD LHSK +E EK S V     S
Sbjct: 1321 PLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVFKEMHDTLHSKFHEGEKISTV----AS 1380

Query: 1689 GEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPIDCDGIYLSS 1748
            GED +SVLHVKYIAALSRELAEN  TSESA NV MP+ES QPT  NEIGPIDCDGIYLSS
Sbjct: 1381 GEDSRSVLHVKYIAALSRELAENRSTSESARNVHMPVESSQPTILNEIGPIDCDGIYLSS 1440

Query: 1749 CGHAVHQGCLDRYLSSLKER------------------------FARRIVFEGGHIVDPE 1808
            CGHAVHQGCLDRYLSSLKE                         FARRIVFEGGHIVDPE
Sbjct: 1441 CGHAVHQGCLDRYLSSLKESSSRAWEGLPALSNFLGGVVLTLKLFARRIVFEGGHIVDPE 1500

Query: 1809 QGEFLCPVCRRLANSTLPAIPREFKKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQA 1868
            QGEFLCPVCRRL+NSTLPA PREF+KIWNP  SS+G+LS VSG LNKS+E VNPLY+Q+A
Sbjct: 1501 QGEFLCPVCRRLSNSTLPAFPREFQKIWNPRTSSVGTLSHVSGHLNKSNERVNPLYIQEA 1560

Query: 1869 VALLQSAAKAVGKSKILKDISVHRHRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPS 1928
            VALLQSAAKAVGK+ +LKDISVHRH+KVSRNLEAVSLVLSKLYFS KQDKLIS +RVNPS
Sbjct: 1561 VALLQSAAKAVGKNNVLKDISVHRHKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPS 1620

Query: 1929 ILMWDTLKYSLISMETAA----------------VELKTSGGFILSLLLKVIQSIKREDS 1988
            ILMWDTLKYSL+SME AA                 ELKTSGGF+LSLLLKVI S+K EDS
Sbjct: 1621 ILMWDTLKYSLVSMEIAARSKTDMNPSIGLNTLYKELKTSGGFVLSLLLKVIHSVKCEDS 1680

Query: 1989 LLLLQRFCGIQRYPSHL--------DKSSCG-------------LPQFDIQFLSRGSDPV 2048
            LLLLQR CGIQR+   +           SCG             LP FD QFLSRGSDPV
Sbjct: 1681 LLLLQRLCGIQRFADSICSGMSNENASDSCGRGILHILTSLRSELPHFDSQFLSRGSDPV 1740

Query: 2049 LAHDPFASLMWVFFCLPCPFLSCKESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFN 2108
            +AHDPFASLMWV FCLP PFLSC+ESLLSLVHIFYLVSV QAIIT   K QCK DGLG +
Sbjct: 1741 IAHDPFASLMWVLFCLPFPFLSCRESLLSLVHIFYLVSVTQAIITYFTKSQCKVDGLGSS 1800

Query: 2109 DCLIADICKIMGESEYARRYFVSNYTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARV 2168
            DCLI DICKIMGESEYAR+YFVSNYTEPSCN+KD +RSLTFP+LRRCALLL+LL+SSARV
Sbjct: 1801 DCLITDICKIMGESEYARQYFVSNYTEPSCNIKDTVRSLTFPFLRRCALLLQLLSSSARV 1860

Query: 2169 PIFDGETAFERYLAVDDMIDNIALELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCH 2228
            PIFDGETA E YL  ++MIDNI +ELNE+EKL+KMF+IPPLDIVLKD  SR LV KWFCH
Sbjct: 1861 PIFDGETALETYLVGNNMIDNITVELNEVEKLQKMFDIPPLDIVLKDGTSRLLVSKWFCH 1920

Query: 2229 FNKEFEFQRSKNLKHCTPAVPFQLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCG 2288
            FNKEFEFQRSK +KHCTPAVPFQLIRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG
Sbjct: 1921 FNKEFEFQRSKIIKHCTPAVPFQLIRLPHVYHDLLQRYIKKRCTDCKHVIDDPALCLICG 1980

Query: 2289 RLCSPSWKSCCRESGCQAHANICAAGT-------EDHNLFQRSARQAPWPSPYLDAFGEE 2303
            +LCSPSWKSCCRESGCQAHANICAAGT           L QRSARQAPWPSPYLDAFGEE
Sbjct: 1981 KLCSPSWKSCCRESGCQAHANICAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEE 2040

BLAST of Sgr022730 vs. ExPASy TrEMBL
Match: A0A0A0LD61 (E3 ubiquitin-protein ligase OS=Cucumis sativus OX=3659 GN=Csa_3G734220 PE=3 SV=1)

HSP 1 Score: 3454.5 bits (8956), Expect = 0.0e+00
Identity = 1758/2041 (86.13%), Postives = 1857/2041 (90.98%), Query Frame = 0

Query: 309  MDDMDIGSPSESVPLKPRDRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAI 368
            MD+MDIGSPSES PLKPRDRI+RRLAVLGVP++LLDQL  GLV+FVKDNKFLI ELVSAI
Sbjct: 1    MDEMDIGSPSESAPLKPRDRILRRLAVLGVPDELLDQLFRGLVTFVKDNKFLIPELVSAI 60

Query: 369  LPTDVEVAEVIRDAKAGSKKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVG 428
            LPTDVEV EVIRDA  G+KKS+ GPTMK  FRESMMWLQWLMF  EP +ALKNLSKMSVG
Sbjct: 61   LPTDVEVVEVIRDAIPGAKKSLAGPTMKANFRESMMWLQWLMFESEPAYALKNLSKMSVG 120

Query: 429  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVT 488
            QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSIIYTGGGCCDCGD T
Sbjct: 121  QRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDCGDAT 180

Query: 489  AWKREGFCSKHKGAEQIQPLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 548
            AWKREGFCSKHKGAEQIQPLPEE+VKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV
Sbjct: 181  AWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRV 240

Query: 549  TEHKKIANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKK 608
            TEHKK+ANELTFAVVEMLLDFCK+SESLLSFVSKRVISSAG LDILVRLERLLTD VVKK
Sbjct: 241  TEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDGVVKK 300

Query: 609  VHELLLKLLGEPIFKYEFAKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPT 668
            VHELLLKLLGEP+FKYEFAKVFLNYYPTVISEAIE+ SDHA KKYPLLPTFSVQIFTVPT
Sbjct: 301  VHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIFTVPT 360

Query: 669  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAV 728
            LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQV+KWSNLYETTIRVVED+RFVMSHAV
Sbjct: 361  LTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVMSHAV 420

Query: 729  VPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 788
            VPRYVIY+QQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL
Sbjct: 421  VPRYVIYQQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSL 480

Query: 789  LVNEAFSAATGST-EETADAMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSSTS 848
            LV EAFSAA+ S+ E++ADAM FQTYKQN +DIDS+RHAKVGRLSQ+SAACNVLG+SS S
Sbjct: 481  LVKEAFSAASSSSCEDSADAMYFQTYKQNVDDIDSVRHAKVGRLSQDSAACNVLGKSSAS 540

Query: 849  TSASRIDEVCSDAISSTIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLYS 908
            TSASR+D+VCSDAISSTIMWLTYECLK+IDSWLGTEN SG IP+M  E+ S+  SCK YS
Sbjct: 541  TSASRVDDVCSDAISSTIMWLTYECLKIIDSWLGTENISGSIPNMLDESISLAPSCKFYS 600

Query: 909  LRKTSALESKKMSSKVEKGKFVFEKLARKSKNYNRQYSSRMYSGLQMSIDHQHGITLGED 968
            LRKTSAL SKK+S K+EKGK  FEKL+R+SK +NRQYSSRMYSGLQMSID++HGI+LGED
Sbjct: 601  LRKTSALASKKLSYKMEKGK--FEKLSRRSKYHNRQYSSRMYSGLQMSIDNEHGISLGED 660

Query: 969  NQLMDVTNDTVTVEDYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKA 1028
            N LMDVTNDTVT EDYAME DALHFLSLS+WPNIVYDVSSQDISIHIPLHRLLSLLLQKA
Sbjct: 661  NHLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLSLLLQKA 720

Query: 1029 LRSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVN 1088
            LRSCFSES VPS+TGASSSNLS EYVDFFKSVLTDCHP+GFS+F+MEHPLRIKVFCAEVN
Sbjct: 721  LRSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPFGFSSFVMEHPLRIKVFCAEVN 780

Query: 1089 TGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDY 1148
             GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLS+Y
Sbjct: 781  AGMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSNY 840

Query: 1149 LSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLV 1208
            LSL+VERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLV
Sbjct: 841  LSLDVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLV 900

Query: 1209 KALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAE 1268
            KALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSL WKYWKELDLYHPRWSLRDLQ+AE
Sbjct: 901  KALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSLRDLQVAE 960

Query: 1269 ERYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRA 1328
            ERYLRSCGVSALTSQLPKWTKIYPPF GLARIATCK  LQ +RAVLFYSVF+E STKSRA
Sbjct: 961  ERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTALQFIRAVLFYSVFSEISTKSRA 1020

Query: 1329 PDSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLL 1388
            PDSVLLSALHLLALALDICFQQKE SDQSF + DSIPLLLFA EE+DEGL YGFGRQSLL
Sbjct: 1021 PDSVLLSALHLLALALDICFQQKESSDQSFDAPDSIPLLLFATEEIDEGLAYGFGRQSLL 1080

Query: 1389 SLLILLMKMH-KKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYL 1448
            SLLILLMKMH KKEGRENLLEAGSCNLSSLVE LLKKFSEIDS CMGKVQQLAPEI GYL
Sbjct: 1081 SLLILLMKMHKKKEGRENLLEAGSCNLSSLVESLLKKFSEIDSHCMGKVQQLAPEILGYL 1140

Query: 1449 SQSVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSV-NDDTEFWQ 1508
            SQSVP+S T+  + TSDSEKRKAKARERQAAILEKMRAEQSKFLAS D+SV +DDTEF Q
Sbjct: 1141 SQSVPTSTTSRPTETSDSEKRKAKARERQAAILEKMRAEQSKFLASVDASVDDDDTEFGQ 1200

Query: 1509 ESEKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDE 1568
            E EKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRG VSWDQPY RDE
Sbjct: 1201 EPEKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGAVSWDQPYCRDE 1260

Query: 1569 HASTMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSH 1628
            H ST SKRDLDQSG+STSS GS VISSPQ +ELIQNAVKEY NHGLPGEVGAFLDFVKSH
Sbjct: 1261 HTSTTSKRDLDQSGVSTSSAGSVVISSPQFSELIQNAVKEYTNHGLPGEVGAFLDFVKSH 1320

Query: 1629 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKLNEDEKFSEVLSGL 1688
            FPPLR+IQVPGTSNVKGEKIIFSFDTLEEDIYLSV KEMHD LHSK N+DEK S+V    
Sbjct: 1321 FPPLRDIQVPGTSNVKGEKIIFSFDTLEEDIYLSVCKEMHDTLHSKFNDDEKISKV---- 1380

Query: 1689 VSGEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPIDCDGIYL 1748
             SG D +SVLHVKYIAALSRELAENH TSESA N+ MP+ESLQPT  NEIGP DCDGIYL
Sbjct: 1381 ASGGDSRSVLHVKYIAALSRELAENHSTSESARNIHMPVESLQPTILNEIGPTDCDGIYL 1440

Query: 1749 SSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANSTLPAIPR 1808
            SSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRL+NSTLPA PR
Sbjct: 1441 SSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLSNSTLPAFPR 1500

Query: 1809 EFKKIWNPSMSSIGSLSRVSGCLNKSSEEVNPLYVQQAVALLQSAAKAVGKSKILKDISV 1868
            EF+KIW+P  SS+G+LS VSG LNKS+E VNPLY+Q+AVALLQSAAKAVGK+ +LKDISV
Sbjct: 1501 EFQKIWSPRTSSVGTLSHVSGHLNKSNERVNPLYIQEAVALLQSAAKAVGKNNVLKDISV 1560

Query: 1869 HRHRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNPSILMWDTLKYSLISMETAA---- 1928
            HRH+KVSRNLEAVSLVLSKLYFS KQDKLIS +RVNPSILMWDTLKYSL+SME AA    
Sbjct: 1561 HRHKKVSRNLEAVSLVLSKLYFSWKQDKLISSSRVNPSILMWDTLKYSLVSMEIAARSKT 1620

Query: 1929 ------------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQRYPSHL----- 1988
                         ELKTSGGF+LSLLLKVIQS+K EDSLLLLQR CGIQR+   +     
Sbjct: 1621 DMNPSIGLNTLYKELKTSGGFVLSLLLKVIQSVKCEDSLLLLQRLCGIQRFADSICSGMS 1680

Query: 1989 ---DKSSCG-------------LPQFDIQFLSRGSDPVLAHDPFASLMWVFFCLPCPFLS 2048
                  SCG             LPQFD QFLSRGSDPV+AHDPFASLMWV FCLP PFLS
Sbjct: 1681 NENASDSCGRGILHILTSLRSELPQFDSQFLSRGSDPVIAHDPFASLMWVLFCLPFPFLS 1740

Query: 2049 CKESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGESEYARRYFV 2108
            C+ESLLSLVHIFYLVSV QAIIT  IK Q + DGLG +DCLI DICKIMGES YAR+YFV
Sbjct: 1741 CRESLLSLVHIFYLVSVTQAIITYFIKSQWEVDGLGSSDCLITDICKIMGESGYARQYFV 1800

Query: 2109 SNYTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLAVDDMIDNI 2168
            SNYTEPSCNVKD +R+LTFPYLRRCALLL+LL+SSARVPIFDGETA E YL  ++MIDNI
Sbjct: 1801 SNYTEPSCNVKDTVRNLTFPYLRRCALLLQLLSSSARVPIFDGETALETYLVGNNMIDNI 1860

Query: 2169 ALELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQRSKNLKHCTPAVPF 2228
             +ELNE+EKL+KMFEIPPLDIVLKDR SR LV KWFCHFNKEFEFQR K +KHCTPAV F
Sbjct: 1861 TVELNEVEKLQKMFEIPPLDIVLKDRTSRLLVSKWFCHFNKEFEFQRFKIIKHCTPAVAF 1920

Query: 2229 QLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWKSCCRESGCQAHANI 2288
            QLIRLPHVYHDLLQRYIKKRC DCKHVIDDPALCL+CG+LCSPSWKSCCRESGCQAHANI
Sbjct: 1921 QLIRLPHVYHDLLQRYIKKRCADCKHVIDDPALCLICGKLCSPSWKSCCRESGCQAHANI 1980

Query: 2289 CAAGT-------EDHNLFQRSARQAPWPSPYLDAFGEEDLEMRRGKPLYLNEERYAALSY 2303
            CAAGT           L QRSARQAPWPSPYLDAFGEED+EMRRGKPLYLNEERYAALSY
Sbjct: 1981 CAAGTGVFLLIRRTTILLQRSARQAPWPSPYLDAFGEEDIEMRRGKPLYLNEERYAALSY 2035

BLAST of Sgr022730 vs. TAIR 10
Match: AT5G02310.1 (proteolysis 6 )

HSP 1 Score: 1757.7 bits (4551), Expect = 0.0e+00
Identity = 1014/2055 (49.34%), Postives = 1318/2055 (64.14%), Query Frame = 0

Query: 327  DRIVRRLAVLGVPEDLLDQLQPGLVSFVKDNKFLITELVSAILPTDVEVAEVIRDAKAGS 386
            D ++ RLA +GVP+    + + GLV FV+ N   I+ELVSA+LPTD +V   +++A+   
Sbjct: 17   DLVIERLASVGVPKKY--RSKRGLVEFVRANPAKISELVSALLPTDDDVKLGLKEARERP 76

Query: 387  KKSVTGPTMKNQFRESMMWLQWLMFGGEPTFALKNLSKMSVGQRGVCGAVWGHNDIAYRC 446
            +KS   PTMK +FRESM  LQWLMF  EP  +L+NL+K+++ QRGVCG+VWG NDIAYRC
Sbjct: 77   RKSAVSPTMKKRFRESMNMLQWLMFQDEPDVSLRNLAKLNLDQRGVCGSVWGQNDIAYRC 136

Query: 447  RTCEHDPTCAICVPCFQSGNHKDHDYSIIYTGGGCCDCGDVTAWKREGFCSKHKGAEQIQ 506
            RTCE+DPTCAICVPCFQ+G+H  HDYSIIYTGGGCCDCGD TAWK +GFCS HKG+EQI+
Sbjct: 137  RTCENDPTCAICVPCFQNGDHNSHDYSIIYTGGGCCDCGDETAWKPDGFCSNHKGSEQIR 196

Query: 507  PLPEEFVKSVGPILDALFTSWKNKLLSAEDISVEDPKLSDRVTEHKKIANELTFAVVEML 566
            PL E    SVGPILDALFT W NKLLSAE    +  + +D +   +K++NELTF VVEML
Sbjct: 197  PLSENLANSVGPILDALFTCWNNKLLSAESSGQKGARSNDTLVILQKMSNELTFIVVEML 256

Query: 567  LDFCKYSESLLSFVSKRVISSAGFLDILVRLERLLTDIVVKKVHELLLKLLGEPIFKYEF 626
            L+F   SESLLSFVS+R+ISS+G L IL++ ER L   V+KK+H+L LKL+G+P+FK EF
Sbjct: 257  LEFSMSSESLLSFVSRRIISSSGLLSILLKAERFLDQDVMKKLHDLFLKLIGDPVFKCEF 316

Query: 627  AKVFLNYYPTVISEAIEEGSDHAFKKYPLLPTFSVQIFTVPTLTPRLVEEMNLLSILLGC 686
            AK F++YYP VISE +++G+D+AFKKYPLL TFSVQI TVPTLTP LV+EMNLL++LLGC
Sbjct: 317  AKAFVSYYPVVISEVVKQGTDNAFKKYPLLSTFSVQILTVPTLTPFLVKEMNLLAMLLGC 376

Query: 687  LEDIFISCVSEDGRLQVIKWSNLYETTIRVVEDIRFVMSHAVVPRYVIYEQQDILRTWLR 746
            L DIF+SC  EDG LQ  K   L ET+ RV+ D++FVMSHA+V +Y  +E +++ R+WL 
Sbjct: 377  LSDIFVSCSGEDGLLQATKLERLCETSERVIGDLKFVMSHAIVSKYATHEHRELSRSWLT 436

Query: 747  LLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVANIHSLLVNEAFSAATGSTEETAD 806
            LLTF QGM+P KRETG+ I+EEN+ +HL F L HS+A IHSLLVN  +SAA+    E  D
Sbjct: 437  LLTFAQGMNPLKRETGIPIDEENDYMHLFFVLGHSIAVIHSLLVNGTYSAASDEEIEN-D 496

Query: 807  AMSFQTYKQNPEDIDSIRHAKVGRLSQESAACNVLGRSST--STSASRIDEV--CSDAIS 866
              + + + +   D D  R+AKVGRLS E + C  +  SS+  S+ AS + ++      + 
Sbjct: 497  RNAKEEFDKC--DGDGERYAKVGRLSHEDSVCTAIVSSSSFDSSMASEVHKIDPFHALLP 556

Query: 867  STIMWLTYECLKVIDSWLGTENTSGGIPDMFAENTSMTSSCKLYSLRKTSALESKKMSSK 926
            S+ ++L  ECLKV+++ LG +    GI             CKL S    +  ESK    +
Sbjct: 557  SSAIYLIRECLKVLETCLGNDE---GISKFL---------CKLSSSSGRNIPESKMSWPR 616

Query: 927  -----VEKGKFVFEKLARKSKNYNRQYSSRMYSGLQ--MSIDH---QHGITLGEDNQLMD 986
                 VE G  V   LA  S++ +   S  +   +Q  +S+D+    +G+         D
Sbjct: 617  RDLLNVETGGSVSSNLASSSRDPSTGLSP-LCGDIQTNLSLDNVCGPYGVV------QTD 676

Query: 987  VTNDTVTVE----DYAMENDALHFLSLSAWPNIVYDVSSQDISIHIPLHRLLSLLLQKAL 1046
            VT D+  V     D       L  L L  WP+I YDVSSQ IS+H+PLHRLLSLL+QKAL
Sbjct: 677  VTADSKRVSCNSADLTKNASGLRILGLCDWPDIHYDVSSQAISVHLPLHRLLSLLIQKAL 736

Query: 1047 RSCFSESVVPSSTGASSSNLSLEYVDFFKSVLTDCHPYGFSAFLMEHPLRIKVFCAEVNT 1106
            R C+ ES   S  G S S+  + + DFF SV+ D HP GFSA +MEH L+I+VFCA+V  
Sbjct: 737  RICYGESA--SYNGVSISH-EIPHADFFSSVIGDFHPCGFSALVMEHVLQIRVFCAQVIA 796

Query: 1107 GMWRRNGDAALLSCELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVSRILERFRLSDYL 1166
            GMW++NGD+AL+SCE YRS+RWSEQ LELDLFLLQCCAA+AP D YV ++L RF LS YL
Sbjct: 797  GMWKKNGDSALVSCEWYRSVRWSEQGLELDLFLLQCCAALAPADSYVDKLLSRFGLSSYL 856

Query: 1167 SLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVK 1226
            SLN +  +EYEP+LVQEML L+IQ++ ERRFCGL+ AESL+RE+I+KLA GD THSQLVK
Sbjct: 857  SLNPDITNEYEPVLVQEMLGLLIQILQERRFCGLSTAESLRREIIFKLATGDFTHSQLVK 916

Query: 1227 ALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLSWKYWKELDLYHPRWSLRDLQIAEE 1286
            +LPRDLSK  +LQE+LD ++VY NPSG NQG YSL    WKELDLYHPRW  RDLQ AEE
Sbjct: 917  SLPRDLSKSDELQEVLDDVSVYCNPSGMNQGKYSLQSSCWKELDLYHPRWQSRDLQSAEE 976

Query: 1287 RYLRSCGVSALTSQLPKWTKIYPPFGGLARIATCKVVLQIVRAVLFYSVFTEKSTKSRAP 1346
            R+ R CGVSALT+QLP+W  IYPP  GLARI TCK   QI+ + L+Y++ +  S KSRAP
Sbjct: 977  RFSRYCGVSALTTQLPRWRMIYPPLKGLARIGTCKATFQIISSALYYALQSGTSVKSRAP 1036

Query: 1347 DSVLLSALHLLALALDICFQQKELSDQSFCSGDSIPLLLFADEEVDEGLTYGFGRQSLLS 1406
            D VL++AL LL+L+LDIC QQ++ + Q  C  +SIP+L  A  E+  G+  G  ++SLLS
Sbjct: 1037 DGVLITALQLLSLSLDICTQQRQSNSQDCCLENSIPILELAGLEI-IGIAQGTEKESLLS 1096

Query: 1407 LLILLMKMHKKEGRENLLEAGSCNLSSLVECLLKKFSEIDSRCMGKVQQLAPEIFGYLSQ 1466
            LL+ LMK    +GR    E GSCN+SS +  LLKKFS IDS CM  +Q LAPE+ G    
Sbjct: 1097 LLVSLMKTRMGDGRHQFPEPGSCNISSWIGNLLKKFSAIDSVCMNLLQSLAPEVVG---- 1156

Query: 1467 SVPSSDTNSLSGTSDSEKRKAKARERQAAILEKMRAEQSKFLASFDSSVNDD---TEFWQ 1526
               S     +SG++  EKRKAKA+ERQAAI+ KM+AEQSKFL++  SS++DD   +EF +
Sbjct: 1157 --QSGFDKVMSGSTSDEKRKAKAKERQAAIMAKMKAEQSKFLSTLSSSMDDDDPRSEF-E 1216

Query: 1527 ESEKPNVSDSAEQSETVCSLCHDSSSSVPISFLILLQKSKLVSLIDRGPVSWDQPYWRDE 1586
             S+     DS      VCSLCHD  S  P+SFLI LQKSKL+S +DRGP SWDQ    ++
Sbjct: 1217 TSDSVMEHDSEIAVREVCSLCHDPDSKDPVSFLIFLQKSKLLSFVDRGPPSWDQCPQSEK 1276

Query: 1587 HASTMSKRDLDQSGLSTSSGGSGVISSPQLAELIQNAVKEYANHGLPGEVGAFLDFVKSH 1646
              S     DL +   S+ S     ISSP + +L  + + E AN          ++ +K+ 
Sbjct: 1277 KISVDGAPDLLRMNASSDSLR---ISSPLMLQLSDDTISESAN---------MIESIKAR 1336

Query: 1647 FPPLRNIQVPGTSNVKGEKIIFSFDTLEEDIYLSVRKEMHDILHSKL-------NEDEKF 1706
               + N Q    S+    K   + ++LE  +Y +VR ++ ++++  L       +E E  
Sbjct: 1337 L--IGNGQTEKRSSDGRGKDESNMESLEIAMYQTVRNKIENMINQSLTRVDHQPHEAENC 1396

Query: 1707 SEVLSGLVSGEDCKSVLHVKYIAALSRELAENHPTSESAHNVSMPIESLQPTTFNEIGPI 1766
            SE  S  V G    S L  ++    SR+ +          +                 PI
Sbjct: 1397 SEKNS--VGG---PSTLQGRFPDIRSRQTSRRPDAGSDGFH-----------------PI 1456

Query: 1767 DCDGIYLSSCGHAVHQGCLDRYLSSLKERFARRIVFEGGHIVDPEQGEFLCPVCRRLANS 1826
            DCDG+YLSSCGHAVHQ CL+RYL SLKER  RR VFEG HIVD ++ EFLCPVCRRLANS
Sbjct: 1457 DCDGVYLSSCGHAVHQSCLERYLKSLKERSGRRTVFEGAHIVDLKKKEFLCPVCRRLANS 1516

Query: 1827 TLPAIPREFKKIWNPSMSSIGSLSRVSGCLNKSSEEVNP-LYVQQAVALLQSAAKAVGKS 1886
             LP  P +        + S+  L        +  + + P L++ +A+ LL+SAA+ +   
Sbjct: 1517 VLPECPGD--------LCSVSKLQDSPRTKLRRKDALQPSLWLSEALCLLRSAAEVIEDG 1576

Query: 1887 KILKDISVHRHRKVSRNLEAVSLVLSKLYFSGKQDKLISFTRVNP-SILMWDTLKYSLIS 1946
               K ++        ++L++VS +L   YF   +DK +    + P SI+MWDTLKYSLIS
Sbjct: 1577 DRGKTVTPQGDGPRRKDLKSVSKMLWDFYFPKPEDKTLKRLWLPPQSIVMWDTLKYSLIS 1636

Query: 1947 METAA-----------------VELKTSGGFILSLLLKVIQSIKREDSLLLLQRFCGIQR 2006
            ME                     ELKTS G ILS+LL+V+QS + ++++ + QRF G++ 
Sbjct: 1637 MEIGTRFAKNSMLPVYCIDSLYEELKTSKGTILSVLLRVVQSSRTKNTIHVRQRFVGMK- 1696

Query: 2007 YPSHLDKSSC-------------------GLPQFDIQFLSRGSDPVLAHDPFASLMWVFF 2066
               HL +S C                    L   D+ + +R SDPVLAHDPF+SLMW  F
Sbjct: 1697 ---HLAESICYGVSSSSSSSIFGSEGTTGSLKNIDLLW-NRASDPVLAHDPFSSLMWALF 1756

Query: 2067 CLPCPFLSCKESLLSLVHIFYLVSVAQAIITSCIKYQCKTDGLGFNDCLIADICKIMGES 2126
            CLP PFL+C+ESLLSLVHIF+ VS+ Q +I  C     +   L F + L+ DI   + ES
Sbjct: 1757 CLPFPFLTCEESLLSLVHIFHSVSLVQTVIAYCACRPSELSELNFGENLLNDISNALRES 1816

Query: 2127 EYARRYFVSNYTEPSCNVKDMIRSLTFPYLRRCALLLKLLNSSARVPIFDGETAFERYLA 2186
                 YF SN  + SC++KD IR  + P+LRRCALL KLL S+ R  + +    F+  L 
Sbjct: 1817 G-GWEYFRSNNMDLSCDIKDTIRKYSLPFLRRCALLWKLLKSTPR-KLHEESDMFD--LP 1876

Query: 2187 VDDMIDNIAL------ELNEIEKLEKMFEIPPLDIVLKDRASRSLVWKWFCHFNKEFEFQ 2246
             D   DN+        ELN +++LEKMF IPP+DI+L D   RS    W  HF +E+   
Sbjct: 1877 SDPTTDNMDFIYSPQSELNHVQELEKMFNIPPIDIILNDELLRSSTQIWLQHFQREYRVN 1936

Query: 2247 RSKNLKHCTPAVPFQLIRLPHVYHDLLQRYIKKRCPDCKHVIDDPALCLLCGRLCSPSWK 2303
            R K     TP VPFQL++LP++Y DLLQR IKKRC +C  VI++P LCLLCG LCSP W 
Sbjct: 1937 RVKRSLCITPVVPFQLMKLPNLYQDLLQRCIKKRCVNCTKVIEEPVLCLLCGSLCSPIWS 1983

BLAST of Sgr022730 vs. TAIR 10
Match: AT2G37630.1 (myb-like HTH transcriptional regulator family protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.3e-40
Identity = 86/114 (75.44%), Postives = 100/114 (87.72%), Query Frame = 0

Query: 164 FCFELVECCRELDEGHRAWAAHKKEAAWRLRRLELQLESEKASRIRQKMEEVEGKVKALR 223
           F  ELVECCREL+EGHRAWA HKKEAAWRLRRLELQLESEK  R R+KMEE+E K+KALR
Sbjct: 250 FLSELVECCRELEEGHRAWADHKKEAAWRLRRLELQLESEKTCRQREKMEEIEAKMKALR 309

Query: 224 EEEKIALERIEAEYKEQLAGLRKDAEAKEQKLAEQWAAKHLSLTKFLE-HMGCR 277
           EE+K A+E+IE EY+EQL GLR+DAEAK+QKLA+QW ++H+ LTKFLE  MGCR
Sbjct: 310 EEQKNAMEKIEGEYREQLVGLRRDAEAKDQKLADQWTSRHIRLTKFLEQQMGCR 363