Sgr022654 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr022654
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionATP-dependent RNA helicase DDX42
Locationtig00000289: 1960046 .. 1963672 (-)
RNA-Seq ExpressionSgr022654
SyntenySgr022654
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGGGCGAGGATGTGGTGAGGAGGAAAAAGAACAAAGCTAGCCGGAAGAAGCTCCGAAGTAAGAACGGAGACTCTTCCTCGTCGGTTTCTGCGCGCGTTGCTGCTATAATCGCCGCCAAGAAGCGGCGTCAGTCCGGTAAACGCAGAAAATGCCTGGTAACTTCCGACGTATTCATTCTTATTTCGTTTCTTCATATTTTTGGATTTACGAAGCTATTTTATGCTTTTAGAGTAGCGTCCTTTGTTTGAGTTGTGATACAAATAAAAGAGGATTATTCAGTTTAGATTTCTCGTCGTGTAGGCGAAGTTAATCTTACTTCAGGGATGCTATAACTTATGATCATAGGTTATTCATTTTATAATACCTTTCATGGTCTTGTTCAATTCGCAGCACAATATGTAAATGCTTGTTAGCAAAGACGATTTTCCGTGCTTGGTTTGTTCATTGAATTAGGTCGCACTTATACTGTAACTCGTAAATGCAGTTTGCTGCTCAAACTACCTGACTTTTGGGACATTGATTAGCCGAGGCCGCCTAAACGTTTCCTTTATTGTTAGTCGTACCCCGTTGATTATTTTCCACTTCCCGCTATTTATTTACTCTGTCGGTGTACTTGATATTTATATTAATCTTAATGGTTGTAAATATATGGAGTTCAAATTTGTTACATTGCAGGGAATGTGCTTTAGCCTTCCTACTCCTGATGATCCCTTTAATGAGAGGAATGGTAATAAAATTTGAATAAGAGGGAAACTAAGGTCACGCTTTCAAAAGCCTCCAAGAGGGCATTTTCAAAGGAGGAGAGTATTTTAGCCTCTAATGGAACCCTTCGAGAGGTTGGCCGTGATAAAGTCAGGAGTTCGAAAGAAGGATTTGGAGATGCCCTCATAATGGCCACATAGCTAAAAGAAGCAAGACTGATCCTGAAAGAACAAATGTTCATGCAAATGTGAAAGGTGGTGTACAGGGCTTTCAAGGAGAAGGCTCTGAAAGTTCTATTTCCCCCTCAAACTTTCTTATACTTTGCTTAAATGCAATAGAGAATGCACTACTTCGTGATTCCATCAACAGAAATGACAAGCCTCTGTTTGCTGATACTTGGGGAATTGAGTTCTGGAAATGTTATTCTTCTGGGAAGGATATTCTGGACGCCAGTGGGTTGTCTTCTACAATGAGCAAATTGCATGGGTGGTTTCTACAGCTGCTGACAGTATTGCTAGGAAAGAGAAAGAAGGTTTATCTTTTAGTAGCCCATATCTTTTATTTCTTGTTCCTACCCAAGAGAAAGCTATTCAGGTTCGCTCCATGTGCAAGCCATTAAAAGCTCTTGGAGTACATACAGTGAGCATACATCCTGGGGCATCCCTAGATCACCAAATTCAAGGGTAAGCTTGCCTATGTATCATGATATATAGTAATAGATTTTTGTTTGAGCCCGAGCTTGGGAAAGATTTTAGAGTACGAATTATTTATTTGTATTTCATCTTGTACCTGCTGGTGTACATACAATGGATCTGGCAGTGATTCCTTGACTTTGTGCCATGTATATGTATTTAGCACTGTGAACAATTTGGACTCTATCTGCTTTTTCTATTTTATATCTAATTGGAAATCTATCTCTCTTCCCATAGTTTGAAGAGCTGTGAACCTGAATTTCTCGTGTCCACACCTGAGAGACTCTTGGAGCTTGTTGCCATGAAGGCTATTGATATTTCTGGTGTATCATTACTGGTAATTTCATAGTCTCTGTTTATTATTTGAAATATCAGTTTGTGTTTTGTTATCCTCTATTTTTCGTGATGACTTTTAATGCTTATAAAAACAATTTTACTGTTTAAAAGTTCTTATTTGGTTATTCACTCTCAGGCTTTATTGATATCCCTCCTACAATGTACTGTCTCAATTATATTCTTCCAATTCAATATCTAAAAAAAAAATCTTCCGATTCAATGTTCTGAAGACGAGCAAGTGGCTTGTTTTCACTTTAGACTTATACAGTATTTCACTAAATGCCTATTGTCAGTAATTTGGACATTCTATCTTAAGATTTGTAATTTGTTGGTTTTTTCTTTTGACATTTTATTAGGTTGTCGATGGACTGGAATCTCTTTCAAGAGGTGGTTATCTTAATATGACACAATCCTTCAGGAAGTCCATATCGAGCAATCTGCATACAATTGTTTTCAGTGATAGCTTCAGCTGTGCCTATGTCCCAGTTGTACAAGACCTCCTCTGGGGCCCAATTCGTAGACTGTCTCTTAATGCTTCTGTTGCCAGTCAAAGTGCCTGCATTATCCAGTCGATAAACTTCTATACTTCTGAAGAGGAAAAGCTATCAAAGGTATGTATGTACATCAACTTCACATATTTTAGTGCAGACTGTGTAGTTCAGGAAATCTTGTTCAGTCTTCTAATACTTTTTTCAAAAGGAAATAAAATTATGATGAATTGGATATGGTTATTGTGAGCCTGACTTCTTTAACCTTAAGGTAGCAGTTATTTGCCAAGTTATGGTCCAAAATGGATAGATGTCTTATCCAAGCTTCTAGGAAACTTTTGATAGTGGATATGACCCTTACATTTTGATTCTGGTCAATCACTTTTCAGCTTTTTACTTTTTAGTTGGTAATTCTTACTCCTAAAGTCATAGTTTGCAGCAATACGAAGAAAAGAGGCTTCTCCTTTTATCATTAATTTTATTAATAGTCATCATTAATGATCTTTGAAGGTCATCCAAGGTTTGGATCAAGCCAAAGGCAGTCAGCTCCACCCACAGCTGTTGAAGGTGCTCTTTATTATTGGGAAAGAGTGTAATGTTCATGACTTAGCTGCTGCTCTTAAATCCAAGGGCCATGACATCTTAGCTGGAGCATTATGTTGTGTCTCAGAATTTAAGAACAAGTAAGCATGCGCATGCCTGTAGCTTGTTTATGCCATTGTAAAGTTTTGTTTCTGAAGCGAATGCATGAACCTTGTTTGAAAACCAAGAAGTGTGTATTTATCAATTTTTTTTAATTTTACTGCATTTAAGTTATTTACAAACGTATCCTTTCTTGATTAGTAGTTCATTCCTTGTCTTCCTACTGGAATTCTGAGTCTTTGATGGGTTTTATGTAGAAGTTAGAGATTGTAAGACATGCATGTAGAAAGTAGGAATTCTCTCCTCGGGAATTTCATTACAATTGCTGATGCCAGAAAAATATCAACTGTCTTTCAACTTTTAATTATTATTAGCCCTCCATCCTCTTCTAGGAAAAAGGGATTGGCAATTGTTCTTCCTCTAATTATTTTTTGTCGAAAATTTTACTCACTATTATTATTATTATTACACGAGCTTCCTAATTGGACCATATTTGTACTACTCTACTAAATCAAATCTATTTTTTTCATATTTGTTCAGTTTGGAAGTAAACAGCAGATTGAGGCCCGTAGTTGCGAAGATTGACATAGAGCAAATTAATACCATAGATCTCGGAAATTATGAAAGCATATTCATACTCGATGCTTTTCCGCCAATAGACAAATATGTTCAGATTCTAACAGGAATGGCCCGACACTCTGTCAGTGGTGTGTTGCACACTTCATTACCAAGGAAGAAGCATCAGTTGCTGGTTCATTAG

mRNA sequence

ATGGCGAAGGGCGAGGATGTGGTGAGGAGGAAAAAGAACAAAGCTAGCCGGAAGAAGCTCCGAAGTAAGAACGGAGACTCTTCCTCGTCGGTTTCTGCGCGCGTTGCTGCTATAATCGCCGCCAAGAAGCGGCGTCAGTCCGGTAAACGCAGAAAATGCCTGGTAACTTCCGACGAGTTCGAAAGAAGGATTTGGAGATGCCCTCATAATGGCCACATAGCTAAAAGAAGCAAGACTGATCCTGAAAGAACAAATGTTCATGCAAATGTGAAAGGTGGTGTACAGGGCTTTCAAGGAGAAGGCTCTGAAAGTTCTATTTCCCCCTCAAACTTTCTTATACTTTGCTTAAATGCAATAGAGAATGCACTACTTCGTGATTCCATCAACAGAAATGACAAGCCTCTGTTTGCTGATACTTGGGGAATTGAGTTCTGGAAATGTTATTCTTCTGGGAAGGATATTCTGGACGCCAGTGGGTTGTCTTCTACAATGAGCAAATTGCATGGGTGGTTTCTACAGCTGCTGACAGTATTGCTAGGAAAGAGAAAGAAGGTTCGCTCCATGTGCAAGCCATTAAAAGCTCTTGGAGTACATACAGTGAGCATACATCCTGGGGCATCCCTAGATCACCAAATTCAAGGTTTGAAGAGCTGTGAACCTGAATTTCTCGTGTCCACACCTGAGAGACTCTTGGAGCTTGTTGCCATGAAGGCTATTGATATTTCTGGTGTATCATTACTGGTTGTCGATGGACTGGAATCTCTTTCAAGAGGTGGTTATCTTAATATGACACAATCCTTCAGGAAGTCCATATCGAGCAATCTGCATACAATTGTTTTCAGTGATAGCTTCAGCTGTGCCTATGTCCCAGTTGTACAAGACCTCCTCTGGGGCCCAATTCGTAGACTGTCTCTTAATGCTTCTGTTGCCAGTCAAAGTGCCTGCATTATCCAGTCGATAAACTTCTATACTTCTGAAGAGGAAAAGCTATCAAAGGTCATCCAAGGTTTGGATCAAGCCAAAGGCAGTCAGCTCCACCCACAGCTGTTGAAGGTGCTCTTTATTATTGGGAAAGAGTGTAATGTTCATGACTTAGCTGCTGCTCTTAAATCCAAGGGCCATGACATCTTAGCTGGAGCATTATGTTGTGTCTCAGAATTTAAGAACAATTTGGAAGTAAACAGCAGATTGAGGCCCGTAGTTGCGAAGATTGACATAGAGCAAATTAATACCATAGATCTCGGAAATTATGAAAGCATATTCATACTCGATGCTTTTCCGCCAATAGACAAATATGTTCAGATTCTAACAGGAATGGCCCGACACTCTGTCAGTGGTGTGTTGCACACTTCATTACCAAGGAAGAAGCATCAGTTGCTGGTTCATTAG

Coding sequence (CDS)

ATGGCGAAGGGCGAGGATGTGGTGAGGAGGAAAAAGAACAAAGCTAGCCGGAAGAAGCTCCGAAGTAAGAACGGAGACTCTTCCTCGTCGGTTTCTGCGCGCGTTGCTGCTATAATCGCCGCCAAGAAGCGGCGTCAGTCCGGTAAACGCAGAAAATGCCTGGTAACTTCCGACGAGTTCGAAAGAAGGATTTGGAGATGCCCTCATAATGGCCACATAGCTAAAAGAAGCAAGACTGATCCTGAAAGAACAAATGTTCATGCAAATGTGAAAGGTGGTGTACAGGGCTTTCAAGGAGAAGGCTCTGAAAGTTCTATTTCCCCCTCAAACTTTCTTATACTTTGCTTAAATGCAATAGAGAATGCACTACTTCGTGATTCCATCAACAGAAATGACAAGCCTCTGTTTGCTGATACTTGGGGAATTGAGTTCTGGAAATGTTATTCTTCTGGGAAGGATATTCTGGACGCCAGTGGGTTGTCTTCTACAATGAGCAAATTGCATGGGTGGTTTCTACAGCTGCTGACAGTATTGCTAGGAAAGAGAAAGAAGGTTCGCTCCATGTGCAAGCCATTAAAAGCTCTTGGAGTACATACAGTGAGCATACATCCTGGGGCATCCCTAGATCACCAAATTCAAGGTTTGAAGAGCTGTGAACCTGAATTTCTCGTGTCCACACCTGAGAGACTCTTGGAGCTTGTTGCCATGAAGGCTATTGATATTTCTGGTGTATCATTACTGGTTGTCGATGGACTGGAATCTCTTTCAAGAGGTGGTTATCTTAATATGACACAATCCTTCAGGAAGTCCATATCGAGCAATCTGCATACAATTGTTTTCAGTGATAGCTTCAGCTGTGCCTATGTCCCAGTTGTACAAGACCTCCTCTGGGGCCCAATTCGTAGACTGTCTCTTAATGCTTCTGTTGCCAGTCAAAGTGCCTGCATTATCCAGTCGATAAACTTCTATACTTCTGAAGAGGAAAAGCTATCAAAGGTCATCCAAGGTTTGGATCAAGCCAAAGGCAGTCAGCTCCACCCACAGCTGTTGAAGGTGCTCTTTATTATTGGGAAAGAGTGTAATGTTCATGACTTAGCTGCTGCTCTTAAATCCAAGGGCCATGACATCTTAGCTGGAGCATTATGTTGTGTCTCAGAATTTAAGAACAATTTGGAAGTAAACAGCAGATTGAGGCCCGTAGTTGCGAAGATTGACATAGAGCAAATTAATACCATAGATCTCGGAAATTATGAAAGCATATTCATACTCGATGCTTTTCCGCCAATAGACAAATATGTTCAGATTCTAACAGGAATGGCCCGACACTCTGTCAGTGGTGTGTTGCACACTTCATTACCAAGGAAGAAGCATCAGTTGCTGGTTCATTAG

Protein sequence

MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKCLVTSDEFERRIWRCPHNGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLILCLNAIENALLRDSINRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGWFLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEFLVSTPERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSCAYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLHPQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRPVVAKIDIEQINTIDLGNYESIFILDAFPPIDKYVQILTGMARHSVSGVLHTSLPRKKHQLLVH
Homology
BLAST of Sgr022654 vs. NCBI nr
Match: XP_022134505.1 (pre-mRNA-processing ATP-dependent RNA helicase prp5 [Momordica charantia])

HSP 1 Score: 678.7 bits (1750), Expect = 3.5e-191
Identity = 382/537 (71.14%), Postives = 408/537 (75.98%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+DV  RK+NKA+RKKLRSK+G SSSSVSARVAAIIAAKKRR+SGKRR+C      L
Sbjct: 1   MAKGDDVAMRKRNKAARKKLRSKDGASSSSVSARVAAIIAAKKRRKSGKRRQCQGMCFSL 60

Query: 61  VTSDE-----------FERRIWRCP----------------------------------- 120
            T D+            +R     P                                   
Sbjct: 61  PTPDDPYNDRNGKKDLNKRETKTTPSKGSKRAFSKDKSTSSPNGTVREVGREKSKPSKEG 120

Query: 121 -----HNGHIAKRSKTDPERTNVHANVKGGVQGFQG--EGSESSISPSNFLILCLNAIEN 180
                 NGHIAKRSKTDPERT VHANVKGGVQ FQ   E SES ISPS FLILCLNAIEN
Sbjct: 121 SGDTLSNGHIAKRSKTDPERTKVHANVKGGVQSFQNQREDSESCISPSKFLILCLNAIEN 180

Query: 181 ALLRDSINRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW----------- 240
           AL  DSINRN KPLFADTWGIEFWKCYSSGKDILD SGLSST  ++  W           
Sbjct: 181 ALHHDSINRNGKPLFADTWGIEFWKCYSSGKDILDTSGLSSTDEQI-AWVVSSAADSIAR 240

Query: 241 -----------FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCE 300
                      +L  L     K  +VRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCE
Sbjct: 241 KEKEGLSFSSPYLLYLVPTQEKATQVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCE 300

Query: 301 PEFLVSTPERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIV 360
           PEFLVSTPERLLELVA+KAIDISGVSLLVVDGLESLSRGGYLN TQS RKSISSNLHTIV
Sbjct: 301 PEFLVSTPERLLELVAVKAIDISGVSLLVVDGLESLSRGGYLNKTQSIRKSISSNLHTIV 360

Query: 361 FSDSFSCAYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQ 420
           FSDSFSCAYVPV+QDLLWGPIRRLSL+ASVASQSAC IQSINFYTSEEEK SKVI+ LDQ
Sbjct: 361 FSDSFSCAYVPVLQDLLWGPIRRLSLDASVASQSACTIQSINFYTSEEEKRSKVIKALDQ 420

Query: 421 AKGSQLHPQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRP 457
           A GSQ  PQLLKVLFI+GKECNVH+LA ALKSKGHDILAGAL  VSEFKNNLEV+SRLRP
Sbjct: 421 ANGSQHRPQLLKVLFILGKECNVHELATALKSKGHDILAGALFGVSEFKNNLEVDSRLRP 480

BLAST of Sgr022654 vs. NCBI nr
Match: XP_038886801.1 (ATP-dependent RNA helicase DBP3 [Benincasa hispida])

HSP 1 Score: 645.2 bits (1663), Expect = 4.3e-181
Identity = 365/534 (68.35%), Postives = 399/534 (74.72%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+DVV RK+NKA+RKKLRSKNGD SSSVSARVAAIIAAKKRR SGKRR+C      L
Sbjct: 1   MAKGDDVVARKRNKANRKKLRSKNGD-SSSVSARVAAIIAAKKRRMSGKRRQCQGMCFSL 60

Query: 61  VTSD----------EFERRIWRCPH----------------------------------- 120
            T D          +F++     P                                    
Sbjct: 61  PTPDDPYNDRNGKKDFKKDTKVTPSKGSKREFPKEKSTSAPNGTLRDVGREKVQSSKEGS 120

Query: 121 -----NGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLILCLNAIENALL 180
                +GH+AKRSKT  ERT V+ANVKG VQGFQ E SESSI+PS FL+LCLNAIE+AL 
Sbjct: 121 GGSLTSGHLAKRSKTSTERTKVYANVKGSVQGFQEENSESSITPSKFLMLCLNAIEDALY 180

Query: 181 RDSINRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW-------------- 240
            DSI R +KPLFADTWGIEFWKCYSSGKDILD SGLSST  K+  W              
Sbjct: 181 HDSIKRINKPLFADTWGIEFWKCYSSGKDILDTSGLSSTDEKI-AWVVSSAADSIARTEK 240

Query: 241 --------FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEF 300
                   +L  L     K  +VRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEF
Sbjct: 241 EGSSFSSPYLLFLVPTQEKATQVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEF 300

Query: 301 LVSTPERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSD 360
           LVSTPERLLELVAM+AIDISGVSLLVVDGLESLSRGGYL+MTQS RKSISS LHTIVFSD
Sbjct: 301 LVSTPERLLELVAMQAIDISGVSLLVVDGLESLSRGGYLDMTQSIRKSISSKLHTIVFSD 360

Query: 361 SFSCAYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKG 420
           SFSCAYVPVVQ LL GPIRRLSLN SVA QSA IIQSINFYTSEEEKLSKVIQ LD+A G
Sbjct: 361 SFSCAYVPVVQSLLGGPIRRLSLNTSVACQSAYIIQSINFYTSEEEKLSKVIQALDRANG 420

Query: 421 SQLHPQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRPVVA 457
           SQL PQ LK+L I+GKECNVH+LAAALKSKGHDI+AGALC V E KNN +V+ RLRPVVA
Sbjct: 421 SQLRPQPLKMLLILGKECNVHELAAALKSKGHDIVAGALCGVPEIKNNSKVDGRLRPVVA 480

BLAST of Sgr022654 vs. NCBI nr
Match: XP_004138675.1 (pre-mRNA-processing ATP-dependent RNA helicase prp5 [Cucumis sativus])

HSP 1 Score: 631.7 bits (1628), Expect = 4.9e-177
Identity = 354/530 (66.79%), Postives = 394/530 (74.34%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+D + RK+NKASRKKLRSK+GD SS+VSARVA+IIAAKKRR SGKRR+C      L
Sbjct: 1   MAKGDDALTRKRNKASRKKLRSKSGD-SSAVSARVASIIAAKKRRMSGKRRQCQGMCFSL 60

Query: 61  VTSD----------------------EF-ERRIWRCPH---------------------- 120
            T D                      EF + +    P+                      
Sbjct: 61  PTPDDPYNDRNGKKDTKVKPSKSSKREFPKEKSTSAPNGTLRDVCFENARSSKEGSGGTL 120

Query: 121 -NGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLILCLNAIENALLRDSI 180
            +G +AKRSKTDPERT  + N KG VQGFQ E SESS++PS FLILCL+AIENAL  DSI
Sbjct: 121 PSGRVAKRSKTDPERTKEYGNAKGSVQGFQEEDSESSVAPSKFLILCLSAIENALYHDSI 180

Query: 181 NRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW------------------ 240
              +KPLFADTWGIEFWKCYSSGKDILD SGLSST  K+  W                  
Sbjct: 181 KSINKPLFADTWGIEFWKCYSSGKDILDTSGLSSTDEKI-AWVVSSAADSIARKEKEGSS 240

Query: 241 ----FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEFLVST 300
               FL  L     K  ++RSMCKPLKALGVHTVSIHPGASL HQIQGLKSCEPEFLVST
Sbjct: 241 FSSPFLLFLVPNQEKATQIRSMCKPLKALGVHTVSIHPGASLGHQIQGLKSCEPEFLVST 300

Query: 301 PERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSC 360
           PERLLELVAM+AIDISGVSLLVVDGLESLSRGGYL+MTQS RKSISS LHTIVFSDSFSC
Sbjct: 301 PERLLELVAMQAIDISGVSLLVVDGLESLSRGGYLDMTQSIRKSISSKLHTIVFSDSFSC 360

Query: 361 AYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLH 420
           AYVP +Q LL GPIRRLSLN SVA QSACIIQSINFYTSE+EKLSKVIQ LD+A GSQ+ 
Sbjct: 361 AYVPFIQSLLGGPIRRLSLNTSVACQSACIIQSINFYTSEKEKLSKVIQALDRANGSQIR 420

Query: 421 PQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRPVVAKIDI 457
           PQ LK+LFI+GKECNVHDLAAALK KGHDI+AGALC V E KNNL+V+ +LRPVVAK DI
Sbjct: 421 PQPLKMLFILGKECNVHDLAAALKFKGHDIVAGALCGVPEIKNNLKVDGKLRPVVAKTDI 480

BLAST of Sgr022654 vs. NCBI nr
Match: XP_023550872.1 (ATP-dependent RNA helicase DDX42 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.9 bits (1605), Expect = 2.3e-174
Identity = 356/534 (66.67%), Postives = 393/534 (73.60%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+D + RK+NK+SRKKLRSKNGD SSSVSARVAAIIAAKKRR+SGKRR C      L
Sbjct: 1   MAKGDDALARKRNKSSRKKLRSKNGD-SSSVSARVAAIIAAKKRRKSGKRRNCQGMCFSL 60

Query: 61  VTSD---------------------------EFER-RIWRCPH----------------- 120
            T D                           EF + +    P+                 
Sbjct: 61  PTLDDPYNDRNGNKDLDKKESKVRPSKGSKREFPKDKSSSAPNGTRGDACREKVRSSKEG 120

Query: 121 -----NGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLILCLNAIENALL 180
                +GHIAKRSKT PERT V+ NV+GGVQ FQ EG +SSISPS FLILCLNAIE AL 
Sbjct: 121 SGNATSGHIAKRSKTCPERTKVNVNVEGGVQNFQEEGFDSSISPSKFLILCLNAIEKALY 180

Query: 181 RDSINRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW-------------- 240
            DS    +KPL AD WGIEFWKCYSSGKDILD SGLSST  K+  W              
Sbjct: 181 HDSFKGINKPLSADAWGIEFWKCYSSGKDILDTSGLSSTDEKI-AWVVSSAADSIARKEK 240

Query: 241 --------FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEF 300
                   +L  L     K  +VRSMCKPLKALGVHTVSIH GASLDHQIQGLKSCEPEF
Sbjct: 241 EGLSFSSPYLLFLVPTQEKATQVRSMCKPLKALGVHTVSIHSGASLDHQIQGLKSCEPEF 300

Query: 301 LVSTPERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSD 360
           LVSTPERLLELVAM+AIDI+GVSLLVVDGL+S S+GGYL M QS RKSISSNLHTIVFSD
Sbjct: 301 LVSTPERLLELVAMQAIDIAGVSLLVVDGLDSFSKGGYLEMIQSIRKSISSNLHTIVFSD 360

Query: 361 SFSCAYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKG 420
           SFSCAYVPVVQDLL GPI+RLSLN SVAS+SACIIQS+NF TSEEEKLSKVIQ LD+A  
Sbjct: 361 SFSCAYVPVVQDLLSGPIQRLSLNTSVASRSACIIQSLNFCTSEEEKLSKVIQALDRAND 420

Query: 421 SQLHPQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRPVVA 457
           SQL PQ LK+LFI+GKECNV +LAAALKSKGHDI+AGALC V E KNN EV+SR RPVVA
Sbjct: 421 SQLCPQPLKMLFILGKECNVQELAAALKSKGHDIVAGALCGVPEIKNNSEVDSRSRPVVA 480

BLAST of Sgr022654 vs. NCBI nr
Match: XP_022993206.1 (ATP-dependent RNA helicase DDX42 [Cucurbita maxima])

HSP 1 Score: 617.5 bits (1591), Expect = 9.6e-173
Identity = 355/534 (66.48%), Postives = 392/534 (73.41%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+D + RK+NKASRKKLRSKNGD SSSVSARVAAIIAAKKRR+SGKRR C      L
Sbjct: 1   MAKGDDALARKRNKASRKKLRSKNGD-SSSVSARVAAIIAAKKRRKSGKRRNCQGMCFSL 60

Query: 61  VTSD---------------------------EFER----------RIWRCPH-------- 120
            T D                           EF +          R   C          
Sbjct: 61  PTLDDPYNDRNGNKDLDKKETKVRPSKGSKREFPKDNSSSAPNGTRGDACREKVRSSKEG 120

Query: 121 -----NGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLILCLNAIENALL 180
                +GHIAKRSKT PERT V+ANV+G VQ FQ EG +SSISPS FLILCLNAIE AL 
Sbjct: 121 SGDATSGHIAKRSKTCPERTKVNANVEGSVQDFQEEGFDSSISPSKFLILCLNAIEKALY 180

Query: 181 RDSINRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW-------------- 240
            DS    ++PL AD WGIEFWKCYSSGKDILD SGLSST  K+  W              
Sbjct: 181 HDSFKGINEPLPADAWGIEFWKCYSSGKDILDTSGLSSTDEKI-AWVVSSAADSIARKEK 240

Query: 241 --------FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEF 300
                   +L  L     K  ++RSMCKPLKALGVHTVSIH GASLDHQIQGLKSCEPEF
Sbjct: 241 EGLSFSSPYLLFLVPTQEKATQIRSMCKPLKALGVHTVSIHSGASLDHQIQGLKSCEPEF 300

Query: 301 LVSTPERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSD 360
           LVSTPERLLELVAM+AIDI+GVSLLVVDGL+S S+GG L M QS RKSISSNL+T VFSD
Sbjct: 301 LVSTPERLLELVAMQAIDIAGVSLLVVDGLDSFSKGGSLEMIQSIRKSISSNLYTFVFSD 360

Query: 361 SFSCAYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKG 420
           SFSCAYVPVVQDLL GPI+RLSLN SVAS+SACIIQSINF+TSEEEKLSKVIQ LD+A G
Sbjct: 361 SFSCAYVPVVQDLLSGPIQRLSLNTSVASRSACIIQSINFFTSEEEKLSKVIQALDRANG 420

Query: 421 SQLHPQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRPVVA 457
           SQL PQ LK+LFI+GKECNV +LAAALKSKGHDI+AGALC V E KNN EV+SR RPVVA
Sbjct: 421 SQLCPQPLKMLFILGKECNVQELAAALKSKGHDIVAGALCGVPELKNNSEVDSRSRPVVA 480

BLAST of Sgr022654 vs. ExPASy Swiss-Prot
Match: Q810A7 (ATP-dependent RNA helicase DDX42 OS=Mus musculus OX=10090 GN=Ddx42 PE=1 SV=3)

HSP 1 Score: 73.9 bits (180), Expect = 5.2e-12
Identity = 66/276 (23.91%), Postives = 126/276 (45.65%), Query Frame = 0

Query: 183 KKVRSMCKPL-KALGVHTVSIHPGASLDHQIQGLKSCEPEFLVSTPERLLELVAMKAIDI 242
           +++ + CK   KA  + +V+++ G S+  Q + L+    E +V TP RL++ V  KA ++
Sbjct: 340 QQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQE-GAEIVVCTPGRLIDHVKKKATNL 399

Query: 243 SGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSCAYVPVVQDLLWGPIR 302
             VS LV D  + +   G+    +S    +  +  T++FS +F      + +D+L  PIR
Sbjct: 400 QRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIR 459

Query: 303 RLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLHPQLLKVLFIIGKECN 362
            +  +   A++   + Q +    S   K + + + L +   S        VL  + K+ N
Sbjct: 460 VVQGDIGEANED--VTQIVEILHSGPSKWNWLTRRLVEFTSSG------SVLLFVTKKAN 519

Query: 363 VHDLAAALKSKGHD--ILAGALCCVSEFKNNLEVNSRLRPVVAKIDIEQINTIDLGNYES 422
             +LA+ LK +GH+  +L G +      K   +   +  PV+   D+     +D+ + ++
Sbjct: 520 AEELASNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDV-AARGLDIPSIKT 579

Query: 423 IFILDAFPPIDKYVQILTGMARHSVSGVLHTSLPRK 456
           +   D    ID +   +    R    GV +T L  K
Sbjct: 580 VINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPK 605

BLAST of Sgr022654 vs. ExPASy Swiss-Prot
Match: Q5F485 (ATP-dependent RNA helicase DDX42 OS=Gallus gallus OX=9031 GN=DDX42 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.2e-11
Identity = 65/276 (23.55%), Postives = 125/276 (45.29%), Query Frame = 0

Query: 183 KKVRSMCKPL-KALGVHTVSIHPGASLDHQIQGLKSCEPEFLVSTPERLLELVAMKAIDI 242
           +++ S CK   KA  + +V+++ G S+  Q + L+    E +V TP RL++ V  KA ++
Sbjct: 340 QQIHSECKRFGKAYNLRSVAVYGGGSMWEQAKALQE-GAEIVVCTPGRLIDHVKKKATNL 399

Query: 243 SGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSCAYVPVVQDLLWGPIR 302
             V+ LV D  + +   G+    +S    +  +  T++FS +F      + +D+L  PIR
Sbjct: 400 QRVTYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIR 459

Query: 303 RLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLHPQLLKVLFIIGKECN 362
            +  +   A++   + Q +  + S   K + + + L +   S        VL  + K+ N
Sbjct: 460 VVQGDIGEANED--VTQIVEIFPSGPSKWNWLTRRLVEFTSSG------SVLLFVTKKAN 519

Query: 363 VHDLAAALKSKGHD--ILAGALCCVSEFKNNLEVNSRLRPVVAKIDIEQINTIDLGNYES 422
             +LA  LK + H+  +L G +      K   E   +  P++   D+     +D+ + ++
Sbjct: 520 AEELANNLKQEDHNLGLLHGDMDQSERNKVISEFKKKGIPILVATDV-AARGLDIPSIKT 579

Query: 423 IFILDAFPPIDKYVQILTGMARHSVSGVLHTSLPRK 456
           +   D    ID +   +    R    GV +T L  K
Sbjct: 580 VINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPK 605

BLAST of Sgr022654 vs. ExPASy Swiss-Prot
Match: Q86XP3 (ATP-dependent RNA helicase DDX42 OS=Homo sapiens OX=9606 GN=DDX42 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.2e-11
Identity = 66/276 (23.91%), Postives = 125/276 (45.29%), Query Frame = 0

Query: 183 KKVRSMCKPL-KALGVHTVSIHPGASLDHQIQGLKSCEPEFLVSTPERLLELVAMKAIDI 242
           +++ + CK   KA  + +V+++ G S+  Q + L+    E +V TP RL++ V  KA ++
Sbjct: 340 QQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQE-GAEIVVCTPGRLIDHVKKKATNL 399

Query: 243 SGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSCAYVPVVQDLLWGPIR 302
             VS LV D  + +   G+    +S    +  +  T++FS +F      + +D+L  PIR
Sbjct: 400 QRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIR 459

Query: 303 RLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLHPQLLKVLFIIGKECN 362
            +  +   A++   + Q +    S   K + + + L +   S        VL  + K+ N
Sbjct: 460 VVQGDIGEANED--VTQIVEILHSGPSKWNWLTRRLVEFTSSG------SVLLFVTKKAN 519

Query: 363 VHDLAAALKSKGHD--ILAGALCCVSEFKNNLEVNSRLRPVVAKIDIEQINTIDLGNYES 422
             +LA  LK +GH+  +L G +      K   +   +  PV+   D+     +D+ + ++
Sbjct: 520 AEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDV-AARGLDIPSIKT 579

Query: 423 IFILDAFPPIDKYVQILTGMARHSVSGVLHTSLPRK 456
           +   D    ID +   +    R    GV +T L  K
Sbjct: 580 VINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPK 605

BLAST of Sgr022654 vs. ExPASy Swiss-Prot
Match: Q7ZY47 (ATP-dependent RNA helicase DDX42 OS=Xenopus laevis OX=8355 GN=ddx42 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.0e-11
Identity = 66/277 (23.83%), Postives = 125/277 (45.13%), Query Frame = 0

Query: 183 KKVRSMCKPL-KALGVHTVSIHPGASLDHQIQGLKSCEPEFLVSTPERLLELVAMKAIDI 242
           +++ S CK   KA  + +V+++ G S+  Q + L+    E +V TP RL++ V  KA ++
Sbjct: 337 QQIHSECKRFGKAYNLRSVAVYGGGSMWEQAKALQE-GAEIVVCTPGRLIDHVKKKATNL 396

Query: 243 SGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSCAYVPVVQDLLWGPIR 302
             V+ LV D  + +   G+    +S    +  +  T++FS +F      + +D+L  PIR
Sbjct: 397 QRVTYLVFDEADRMFDMGFEYQVRSIANHVRPDRQTLLFSATFRKKIEKLARDILVDPIR 456

Query: 303 RLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLHPQLLKVLFIIGKECN 362
            +  +   A++   I Q +    S  EK + + + L +   +        VL  + K+ N
Sbjct: 457 VVQGDIGEANED--ITQVVEILPSGPEKWTWLTRRLVEFTSTG------SVLVFVTKKAN 516

Query: 363 VHDLAAALKSKGH--DILAGALCCVSEFKNNLEVNSRLRPVVAKIDIEQINTIDLGNYES 422
             +LAA L+   H   +L G +      K   +   +  PV+   D+     +D+ + ++
Sbjct: 517 AEELAANLRQDDHPLGLLHGDMDQSERNKVISDFKKKSIPVLVATDV-AARGLDIPSIKT 576

Query: 423 IFILDAFPPIDKYVQILTGMARHSVSGVLHTSLPRKK 457
           +   D    ID +   +    R    GV +T L  K+
Sbjct: 577 VVNYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTSKE 603

BLAST of Sgr022654 vs. ExPASy Swiss-Prot
Match: Q5R7D1 (ATP-dependent RNA helicase DDX42 OS=Pongo abelii OX=9601 GN=DDX42 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 3.4e-11
Identity = 64/265 (24.15%), Postives = 119/265 (44.91%), Query Frame = 0

Query: 193 KALGVHTVSIHPGASLDHQIQGLKSCEPEFLVSTPERLLELVAMKAIDISGVSLLVVDGL 252
           KA  + +V+++ G S+  Q + L+    E +V TP RL++ V  KA ++  VS LV D  
Sbjct: 351 KAYNLRSVAVYGGGSMWEQAKALQE-GAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEA 410

Query: 253 ESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSCAYVPVVQDLLWGPIRRLSLNASVASQ 312
           + +   G+    +S    +  +  T++FS +F      + +D+L  PIR +  +   A++
Sbjct: 411 DRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANE 470

Query: 313 SACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLHPQLLKVLFIIGKECNVHDLAAALKSK 372
              + Q +    S   K + + + L +   S        VL  + K+ N  +LA  LK +
Sbjct: 471 D--VTQIVEILHSGPSKWNWLTRRLVEFTSSG------SVLLFVTKKANAEELANNLKQE 530

Query: 373 GHD--ILAGALCCVSEFKNNLEVNSRLRPVVAKIDIEQINTIDLGNYESIFILDAFPPID 432
           GH+  +L G +      K   +   +  PV+   D+     +D+ + +++   D    ID
Sbjct: 531 GHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDV-AARGLDIPSIKTVINYDVARDID 590

Query: 433 KYVQILTGMARHSVSGVLHTSLPRK 456
            +   +    R    GV +T L  K
Sbjct: 591 THTHRIGRTGRAGEKGVAYTLLTPK 605

BLAST of Sgr022654 vs. ExPASy TrEMBL
Match: A0A6J1BYG8 (pre-mRNA-processing ATP-dependent RNA helicase prp5 OS=Momordica charantia OX=3673 GN=LOC111006731 PE=4 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.7e-191
Identity = 382/537 (71.14%), Postives = 408/537 (75.98%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+DV  RK+NKA+RKKLRSK+G SSSSVSARVAAIIAAKKRR+SGKRR+C      L
Sbjct: 1   MAKGDDVAMRKRNKAARKKLRSKDGASSSSVSARVAAIIAAKKRRKSGKRRQCQGMCFSL 60

Query: 61  VTSDE-----------FERRIWRCP----------------------------------- 120
            T D+            +R     P                                   
Sbjct: 61  PTPDDPYNDRNGKKDLNKRETKTTPSKGSKRAFSKDKSTSSPNGTVREVGREKSKPSKEG 120

Query: 121 -----HNGHIAKRSKTDPERTNVHANVKGGVQGFQG--EGSESSISPSNFLILCLNAIEN 180
                 NGHIAKRSKTDPERT VHANVKGGVQ FQ   E SES ISPS FLILCLNAIEN
Sbjct: 121 SGDTLSNGHIAKRSKTDPERTKVHANVKGGVQSFQNQREDSESCISPSKFLILCLNAIEN 180

Query: 181 ALLRDSINRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW----------- 240
           AL  DSINRN KPLFADTWGIEFWKCYSSGKDILD SGLSST  ++  W           
Sbjct: 181 ALHHDSINRNGKPLFADTWGIEFWKCYSSGKDILDTSGLSSTDEQI-AWVVSSAADSIAR 240

Query: 241 -----------FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCE 300
                      +L  L     K  +VRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCE
Sbjct: 241 KEKEGLSFSSPYLLYLVPTQEKATQVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCE 300

Query: 301 PEFLVSTPERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIV 360
           PEFLVSTPERLLELVA+KAIDISGVSLLVVDGLESLSRGGYLN TQS RKSISSNLHTIV
Sbjct: 301 PEFLVSTPERLLELVAVKAIDISGVSLLVVDGLESLSRGGYLNKTQSIRKSISSNLHTIV 360

Query: 361 FSDSFSCAYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQ 420
           FSDSFSCAYVPV+QDLLWGPIRRLSL+ASVASQSAC IQSINFYTSEEEK SKVI+ LDQ
Sbjct: 361 FSDSFSCAYVPVLQDLLWGPIRRLSLDASVASQSACTIQSINFYTSEEEKRSKVIKALDQ 420

Query: 421 AKGSQLHPQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRP 457
           A GSQ  PQLLKVLFI+GKECNVH+LA ALKSKGHDILAGAL  VSEFKNNLEV+SRLRP
Sbjct: 421 ANGSQHRPQLLKVLFILGKECNVHELATALKSKGHDILAGALFGVSEFKNNLEVDSRLRP 480

BLAST of Sgr022654 vs. ExPASy TrEMBL
Match: A0A0A0LQJ4 (DEAD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G386150 PE=4 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 2.4e-177
Identity = 354/530 (66.79%), Postives = 394/530 (74.34%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+D + RK+NKASRKKLRSK+GD SS+VSARVA+IIAAKKRR SGKRR+C      L
Sbjct: 1   MAKGDDALTRKRNKASRKKLRSKSGD-SSAVSARVASIIAAKKRRMSGKRRQCQGMCFSL 60

Query: 61  VTSD----------------------EF-ERRIWRCPH---------------------- 120
            T D                      EF + +    P+                      
Sbjct: 61  PTPDDPYNDRNGKKDTKVKPSKSSKREFPKEKSTSAPNGTLRDVCFENARSSKEGSGGTL 120

Query: 121 -NGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLILCLNAIENALLRDSI 180
            +G +AKRSKTDPERT  + N KG VQGFQ E SESS++PS FLILCL+AIENAL  DSI
Sbjct: 121 PSGRVAKRSKTDPERTKEYGNAKGSVQGFQEEDSESSVAPSKFLILCLSAIENALYHDSI 180

Query: 181 NRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW------------------ 240
              +KPLFADTWGIEFWKCYSSGKDILD SGLSST  K+  W                  
Sbjct: 181 KSINKPLFADTWGIEFWKCYSSGKDILDTSGLSSTDEKI-AWVVSSAADSIARKEKEGSS 240

Query: 241 ----FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEFLVST 300
               FL  L     K  ++RSMCKPLKALGVHTVSIHPGASL HQIQGLKSCEPEFLVST
Sbjct: 241 FSSPFLLFLVPNQEKATQIRSMCKPLKALGVHTVSIHPGASLGHQIQGLKSCEPEFLVST 300

Query: 301 PERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSC 360
           PERLLELVAM+AIDISGVSLLVVDGLESLSRGGYL+MTQS RKSISS LHTIVFSDSFSC
Sbjct: 301 PERLLELVAMQAIDISGVSLLVVDGLESLSRGGYLDMTQSIRKSISSKLHTIVFSDSFSC 360

Query: 361 AYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLH 420
           AYVP +Q LL GPIRRLSLN SVA QSACIIQSINFYTSE+EKLSKVIQ LD+A GSQ+ 
Sbjct: 361 AYVPFIQSLLGGPIRRLSLNTSVACQSACIIQSINFYTSEKEKLSKVIQALDRANGSQIR 420

Query: 421 PQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRPVVAKIDI 457
           PQ LK+LFI+GKECNVHDLAAALK KGHDI+AGALC V E KNNL+V+ +LRPVVAK DI
Sbjct: 421 PQPLKMLFILGKECNVHDLAAALKFKGHDIVAGALCGVPEIKNNLKVDGKLRPVVAKTDI 480

BLAST of Sgr022654 vs. ExPASy TrEMBL
Match: A0A6J1JVP3 (ATP-dependent RNA helicase DDX42 OS=Cucurbita maxima OX=3661 GN=LOC111489296 PE=4 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 4.6e-173
Identity = 355/534 (66.48%), Postives = 392/534 (73.41%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+D + RK+NKASRKKLRSKNGD SSSVSARVAAIIAAKKRR+SGKRR C      L
Sbjct: 1   MAKGDDALARKRNKASRKKLRSKNGD-SSSVSARVAAIIAAKKRRKSGKRRNCQGMCFSL 60

Query: 61  VTSD---------------------------EFER----------RIWRCPH-------- 120
            T D                           EF +          R   C          
Sbjct: 61  PTLDDPYNDRNGNKDLDKKETKVRPSKGSKREFPKDNSSSAPNGTRGDACREKVRSSKEG 120

Query: 121 -----NGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLILCLNAIENALL 180
                +GHIAKRSKT PERT V+ANV+G VQ FQ EG +SSISPS FLILCLNAIE AL 
Sbjct: 121 SGDATSGHIAKRSKTCPERTKVNANVEGSVQDFQEEGFDSSISPSKFLILCLNAIEKALY 180

Query: 181 RDSINRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW-------------- 240
            DS    ++PL AD WGIEFWKCYSSGKDILD SGLSST  K+  W              
Sbjct: 181 HDSFKGINEPLPADAWGIEFWKCYSSGKDILDTSGLSSTDEKI-AWVVSSAADSIARKEK 240

Query: 241 --------FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEF 300
                   +L  L     K  ++RSMCKPLKALGVHTVSIH GASLDHQIQGLKSCEPEF
Sbjct: 241 EGLSFSSPYLLFLVPTQEKATQIRSMCKPLKALGVHTVSIHSGASLDHQIQGLKSCEPEF 300

Query: 301 LVSTPERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSD 360
           LVSTPERLLELVAM+AIDI+GVSLLVVDGL+S S+GG L M QS RKSISSNL+T VFSD
Sbjct: 301 LVSTPERLLELVAMQAIDIAGVSLLVVDGLDSFSKGGSLEMIQSIRKSISSNLYTFVFSD 360

Query: 361 SFSCAYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKG 420
           SFSCAYVPVVQDLL GPI+RLSLN SVAS+SACIIQSINF+TSEEEKLSKVIQ LD+A G
Sbjct: 361 SFSCAYVPVVQDLLSGPIQRLSLNTSVASRSACIIQSINFFTSEEEKLSKVIQALDRANG 420

Query: 421 SQLHPQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRPVVA 457
           SQL PQ LK+LFI+GKECNV +LAAALKSKGHDI+AGALC V E KNN EV+SR RPVVA
Sbjct: 421 SQLCPQPLKMLFILGKECNVQELAAALKSKGHDIVAGALCGVPELKNNSEVDSRSRPVVA 480

BLAST of Sgr022654 vs. ExPASy TrEMBL
Match: A0A5D3CIJ6 (Pre-mRNA-processing ATP-dependent RNA helicase prp5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold304G00610 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 7.9e-173
Identity = 348/530 (65.66%), Postives = 392/530 (73.96%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+D + RK+NKA+RKKLRSK+GD SSSVSAR+A+IIAAKKRR SGKRR+C      L
Sbjct: 1   MAKGDDALTRKRNKAARKKLRSKSGD-SSSVSARIASIIAAKKRRMSGKRRQCQGMCFSL 60

Query: 61  VTSD----------------------EF-ERRIWRCPH---------------------- 120
            T D                      EF + +    P+                      
Sbjct: 61  PTPDDPYNDRNGKKDTKVKPSKGSKREFPKEKSTSAPNGTLRDVGRENARSSKEGSGGTL 120

Query: 121 -NGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLILCLNAIENALLRDSI 180
            +G +AKRSKTDPERT    N KG VQGFQ E SESS++PS FLILCLNAIENAL  DSI
Sbjct: 121 PSGRLAKRSKTDPERTKEFGNAKGSVQGFQEEDSESSVAPSKFLILCLNAIENALYHDSI 180

Query: 181 NRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW------------------ 240
            R +KPLFADTWGIEFWKCYSSGKDILD SGLSST  K+  W                  
Sbjct: 181 KRINKPLFADTWGIEFWKCYSSGKDILDTSGLSSTDEKI-AWVVSSAADSIARKDKEGSS 240

Query: 241 ----FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEFLVST 300
               +L  L     K  ++RSMCKPLKALGVHTVSIHPGASL HQIQGLKSCEPEFLVST
Sbjct: 241 FSSPYLLFLVPNQEKATQIRSMCKPLKALGVHTVSIHPGASLGHQIQGLKSCEPEFLVST 300

Query: 301 PERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSC 360
           PERLLELVAM+AIDISGVSLLVVDGLESLS+GGYL+MTQS RKSISS LHTIVFSDSFS 
Sbjct: 301 PERLLELVAMQAIDISGVSLLVVDGLESLSKGGYLDMTQSIRKSISSKLHTIVFSDSFSR 360

Query: 361 AYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLH 420
           AYVPV+Q LL GP+RRLSLN SVA QSACIIQSINFYTSE+EKLSKVIQ LD+A GS L 
Sbjct: 361 AYVPVIQSLLGGPVRRLSLNTSVACQSACIIQSINFYTSEKEKLSKVIQALDRANGSPLC 420

Query: 421 PQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRPVVAKIDI 457
           PQ LK+LFI+GKECNVH+LAAALKSKGHDI+AGALC V E KNN +V+ +LR VVAK  I
Sbjct: 421 PQPLKMLFILGKECNVHELAAALKSKGHDIVAGALCGVPEIKNNSKVDGKLRYVVAKTHI 480

BLAST of Sgr022654 vs. ExPASy TrEMBL
Match: A0A1S3C3J7 (pre-mRNA-processing ATP-dependent RNA helicase prp5 OS=Cucumis melo OX=3656 GN=LOC103496489 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 7.9e-173
Identity = 348/530 (65.66%), Postives = 392/530 (73.96%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKC------L 60
           MAKG+D + RK+NKA+RKKLRSK+GD SSSVSAR+A+IIAAKKRR SGKRR+C      L
Sbjct: 1   MAKGDDALTRKRNKAARKKLRSKSGD-SSSVSARIASIIAAKKRRMSGKRRQCQGMCFSL 60

Query: 61  VTSD----------------------EF-ERRIWRCPH---------------------- 120
            T D                      EF + +    P+                      
Sbjct: 61  PTPDDPYNDRNGKKDTKVKPSKGSKREFPKEKSTSAPNGTLRDVGRENARSSKEGSGGTL 120

Query: 121 -NGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLILCLNAIENALLRDSI 180
            +G +AKRSKTDPERT    N KG VQGFQ E SESS++PS FLILCLNAIENAL  DSI
Sbjct: 121 PSGRLAKRSKTDPERTKEFGNAKGSVQGFQEEDSESSVAPSKFLILCLNAIENALYHDSI 180

Query: 181 NRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW------------------ 240
            R +KPLFADTWGIEFWKCYSSGKDILD SGLSST  K+  W                  
Sbjct: 181 KRINKPLFADTWGIEFWKCYSSGKDILDTSGLSSTDEKI-AWVVSSAADSIARKDKEGSS 240

Query: 241 ----FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIHPGASLDHQIQGLKSCEPEFLVST 300
               +L  L     K  ++RSMCKPLKALGVHTVSIHPGASL HQIQGLKSCEPEFLVST
Sbjct: 241 FSSPYLLFLVPNQEKATQIRSMCKPLKALGVHTVSIHPGASLGHQIQGLKSCEPEFLVST 300

Query: 301 PERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSC 360
           PERLLELVAM+AIDISGVSLLVVDGLESLS+GGYL+MTQS RKSISS LHTIVFSDSFS 
Sbjct: 301 PERLLELVAMQAIDISGVSLLVVDGLESLSKGGYLDMTQSIRKSISSKLHTIVFSDSFSR 360

Query: 361 AYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLH 420
           AYVPV+Q LL GP+RRLSLN SVA QSACIIQSINFYTSE+EKLSKVIQ LD+A GS L 
Sbjct: 361 AYVPVIQSLLGGPVRRLSLNTSVACQSACIIQSINFYTSEKEKLSKVIQALDRANGSPLC 420

Query: 421 PQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCCVSEFKNNLEVNSRLRPVVAKIDI 457
           PQ LK+LFI+GKECNVH+LAAALKSKGHDI+AGALC V E KNN +V+ +LR VVAK  I
Sbjct: 421 PQPLKMLFILGKECNVHELAAALKSKGHDIVAGALCGVPEIKNNSKVDGKLRYVVAKTHI 480

BLAST of Sgr022654 vs. TAIR 10
Match: AT2G28600.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 1.2e-93
Identity = 211/492 (42.89%), Postives = 286/492 (58.13%), Query Frame = 0

Query: 1   MAKGEDVVRRKKNKASRKKLRSKNGDSSSSVSARVAAIIAAKKRRQSGKRRKCL------ 60
           MAKG+D V+RKKNK +RKK+  KN   +++VSAR+AAIIAAKKRR+SGKR  C       
Sbjct: 1   MAKGDDNVQRKKNKVTRKKMSRKN--DTATVSARIAAIIAAKKRRKSGKRSMCQGMCFTL 60

Query: 61  -VTSDEFERRIWRCPHNGHIAKRSKTDPERTNVHANVKGGVQGFQGEGSESSISPSNFLI 120
               D F  R         I K+ K   ++  V +           EG E    P  FL+
Sbjct: 61  PTLEDPFNER----QGKADITKKKK---KKKKVKSREDKKPSPMSIEGVEKMDGPPKFLM 120

Query: 121 LCLNAIENALLRDSI--NRNDKPLFADTWGIEFWKCYSSGKDILDASGLSSTMSKLHGW- 180
           L LN IE++  +D     ++DK LF  +WGIEFWKCYSSG DILD SG+SST+ ++  W 
Sbjct: 121 LNLNEIESSFRKDITYSEQHDKSLFTSSWGIEFWKCYSSGNDILDTSGMSSTVEQI-AWI 180

Query: 181 ---------------------------FLQLLTVLLGKRKKVRSMCKPLKALGVHTVSIH 240
                                      FL  L     K  +VRS+CK LK +G+HTVS+H
Sbjct: 181 VSTAADAIARREKDEVEEEEELLGNSPFLLYLVPSQSKASQVRSVCKALKGIGIHTVSLH 240

Query: 241 PGASLDHQIQGLKSCEPEFLVSTPERLLELVAMKAIDISGVSLLVVDGLESLSRGGYLNM 300
            GA LDHQI GLKS EPEF+V+TPERLLE+V +K +DIS VSLLV+D L SL  GGYLN 
Sbjct: 241 QGAPLDHQISGLKSVEPEFIVATPERLLEIVTLKGVDISNVSLLVIDELGSLCSGGYLNA 300

Query: 301 TQSFRKSISSNLHTIVFSDSFSCAYVPVVQDLLWGPIRRLSLNASVASQSACIIQSINFY 360
            +S +++ISS   TIVF++SFS + +P VQ  L G + R+++N SVASQ +CI Q+++  
Sbjct: 301 VKSIKQAISSKHQTIVFNNSFSASIIPAVQSFLGGSVNRVTVNESVASQGSCITQTVSVC 360

Query: 361 TSEEEKLSKVIQGLDQAKGSQLHPQLLKVLFIIGKECNVHDLAAALKSKGHDILAGALCC 420
            SEE+KL K  + LD +          K+++I+ KE +   + A LK KG  +   +   
Sbjct: 361 ASEEKKLQKFAKHLDSSSS--------KLIYIVTKEESFKKIMAILKLKGISVSTSSDSK 420

Query: 421 VSEFKNNLEVNSRLRPVVAKIDIEQINTIDLGNYESIFILDAFPPIDKYVQILTGMARHS 456
           +SE K +       +PV   ID EQ++T  + + E++ + D FP I+ Y QILT MAR S
Sbjct: 421 LSEVKKS------RKPVAHLIDFEQLDTTVMRDSETVLLPDFFPSIEIYTQILTSMARES 468

BLAST of Sgr022654 vs. TAIR 10
Match: AT2G47330.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-08
Identity = 64/272 (23.53%), Postives = 124/272 (45.59%), Query Frame = 0

Query: 193 KALGVHTVSIHPGASLDHQIQGLKS-CEPEFLVSTPERLLELVAMKAIDISGVSLLVVDG 252
           KA G+   +++ G S   Q + LK+ C  E +V+TP RL++++ MKA+ +   S LV+D 
Sbjct: 326 KAYGLRVSAVYGGMSKHEQFKELKAGC--EIVVATPGRLIDMLKMKALTMMRASYLVLDE 385

Query: 253 LESLSRGGYLNMTQSFRKSISSNLHTIVFSDSFSCAYVPVVQDLLWGPIRRLSLNASVAS 312
            + +   G+    +S    I  +  T++FS +       + +++L  PIR       +A+
Sbjct: 386 ADRMFDLGFEPQVRSIVGQIRPDRQTLLFSATMPWKVEKLAREILSDPIRVTVGEVGMAN 445

Query: 313 QSACIIQSINFYTSEEEKLSKVIQGLDQAKGSQLHPQLL---KVLFIIGKECNVHDLAAA 372
           +   I Q +N   S+ EKL  +++ L         P ++    VL    K+  V ++ A 
Sbjct: 446 ED--ITQVVNVIPSDAEKLPWLLEKL---------PGMIDEGDVLVFASKKATVDEIEAQ 505

Query: 373 LKSKGHDILA--GALCCVSEFKNNLEVNSRLRPVVAKIDIEQINTIDLGNYESIFILDAF 432
           L      + A  G     S  +   +  S +  V+   D+     +D+ + +++   D  
Sbjct: 506 LTLNSFKVAALHGDKDQASRMETLQKFKSGVHHVLIATDV-AARGLDIKSLKTVVNYDIA 565

Query: 433 PPIDKYVQIL--TGMARHSVSGVLHTSLPRKK 457
             +D +V  +  TG A     GV +T + +++
Sbjct: 566 KDMDMHVHRIGRTGRAGDR-DGVAYTLVTQRE 582

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134505.13.5e-19171.14pre-mRNA-processing ATP-dependent RNA helicase prp5 [Momordica charantia][more]
XP_038886801.14.3e-18168.35ATP-dependent RNA helicase DBP3 [Benincasa hispida][more]
XP_004138675.14.9e-17766.79pre-mRNA-processing ATP-dependent RNA helicase prp5 [Cucumis sativus][more]
XP_023550872.12.3e-17466.67ATP-dependent RNA helicase DDX42 [Cucurbita pepo subsp. pepo][more]
XP_022993206.19.6e-17366.48ATP-dependent RNA helicase DDX42 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q810A75.2e-1223.91ATP-dependent RNA helicase DDX42 OS=Mus musculus OX=10090 GN=Ddx42 PE=1 SV=3[more]
Q5F4851.2e-1123.55ATP-dependent RNA helicase DDX42 OS=Gallus gallus OX=9031 GN=DDX42 PE=2 SV=1[more]
Q86XP31.2e-1123.91ATP-dependent RNA helicase DDX42 OS=Homo sapiens OX=9606 GN=DDX42 PE=1 SV=1[more]
Q7ZY472.0e-1123.83ATP-dependent RNA helicase DDX42 OS=Xenopus laevis OX=8355 GN=ddx42 PE=2 SV=1[more]
Q5R7D13.4e-1124.15ATP-dependent RNA helicase DDX42 OS=Pongo abelii OX=9601 GN=DDX42 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BYG81.7e-19171.14pre-mRNA-processing ATP-dependent RNA helicase prp5 OS=Momordica charantia OX=36... [more]
A0A0A0LQJ42.4e-17766.79DEAD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G386150 PE=4 S... [more]
A0A6J1JVP34.6e-17366.48ATP-dependent RNA helicase DDX42 OS=Cucurbita maxima OX=3661 GN=LOC111489296 PE=... [more]
A0A5D3CIJ67.9e-17365.66Pre-mRNA-processing ATP-dependent RNA helicase prp5 OS=Cucumis melo var. makuwa ... [more]
A0A1S3C3J77.9e-17365.66pre-mRNA-processing ATP-dependent RNA helicase prp5 OS=Cucumis melo OX=3656 GN=L... [more]
Match NameE-valueIdentityDescription
AT2G28600.11.2e-9342.89P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G47330.11.2e-0823.53P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 189..281
e-value: 8.1E-7
score: 29.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 177..306
e-value: 1.4E-12
score: 49.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 180..298
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 98..164
coord: 182..452
NoneNo IPR availablePANTHERPTHR47958:SF8ATP-DEPENDENT RNA HELICASE DBP3 ISOFORM X1coord: 98..164
coord: 182..452

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr022654.1Sgr022654.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009987 cellular process
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0015276 ligand-gated ion channel activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0038023 signaling receptor activity