Sgr022642 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr022642
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionnifU-like protein 4, mitochondrial
Locationtig00000289: 1879906 .. 1887250 (+)
RNA-Seq ExpressionSgr022642
SyntenySgr022642
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGGGCTTAGGGAGCTTACTTGGGCGAGCTCGGAGGATACCTAGATTGGATTCTCGCGGAGGCGGTAAGATCCGGCGCCTCTCCGCCGCAACAACTTCTTCTCTGTTCGGCGGCGAAGACGCTGCTTCGTGTTCTGCGTCGTCTAGGTCGTCTTCTTCATTCACTTCTTCTTTGCGCTCTTGGAAATGGAACCACTTCAAAGGTGCGCTGATGAAGTCATCGATGGACAAATTGCATTCCCTTTAATGTATTTTTTTTGCACGGTGGTATATGGAGGGGCATTTGTAACTCCTATGTTGTTCGTTTGCTTCCTTTATTTATCTTTCTATTACATTTAGGACAGAGGAGAACAATGTTTATTCAAACTCAATCAACACCCAATCCTTCGTCTCTCATGTTCCATCCTGGAAAGCCTGTTATGGAAGTTGGGAGTGCAGATTTTCCGAATGCGCGTTCTGCTATGAACTCCCCGCTAGCGAAAGCCCTTTATGGCATTGATGGTACATCTTCTGAACTTATATATCTTTTTGGATTTCTGTTGGACTTTCAATTTGGAAAACCCTTTTTCTTTCACCCGTGTTTTAAGGAATTTAACATGCATACTTGCATTTTCCAACGCGTCAAAGAGATCATAGCTCGAAATATATCAATGATGACGTCGAGGGTTTTCTTTCCCCTTTTCTGCATGTAAATTGCTGCTGTTTTTCTCTTTTCCATCTTTGCAAGGTCTAGGTTGCGGATGGTTTCCTTACCAAAACTAACAATGCAATTGTATCAATTCTAATGATAATTTTATCACTTCTTGTTATCCCATATGTAAGGACATCGTTGTATTGTTAATTTTAATTTTAATTGCTACAAGCAGTTGATTTGGCGATGGTTTACAAGTATACTTAGTGGTTTTTTTATGGTTGGCGTGGGGCTTACTTGATTGCTTGTGCAGGTATTATCCGAGTCTTCTTTGGATCGGATTTTGTTACTGTAACAAAATCAGAAGATGCTTCTTGGGATTTTTTAAAGCCTGAAATATTTGCCGCAATCATGGACTTCTATTCTTCTGGGCAACCACTATTTCTGGACTCTAAAACTGCTGCCGCCATGGACACAGCCATCAATGAAGTATGTCTTCCAACTGTTGCTCATTAAGAAATAAATTAATTTTTTACATCGCATATTGTTCTCTTTTCTCCGGACTTTTTAATATTAGGTTCAACAAGTTGATGCGCTTACATTTTTTTCTCTGGGGATATTCATTATAATTTTTTATTAGGCTGTGTTTTGATTGCAAATTATTCACTTCTATTTTGTTCTCTTTCCTCTCTAGCTGTACCTGTTCATTTAATACAAGGTAAGAATGAGAGTTCCTTAGCCAAACCACGCTAGTCAATATATGTAGATTTTTAACTGTTTCTTAAATACTAATATAAAAATTTGAACATTTAATATCATCATACTTAAGATTAAAGTAATCTGGGCTCATGGTATCATTAGCATAATGTGGTGCCTTGCAGTTCTATTCACGGTGATCTTACTTCTTAGTTCTATTGAGGACTTGGGCACTGCATTCTTTTTGAACTTGCAATATTATAATCTTGACTTACTGACTCTTCTGGATATAACTTTCGCTAAGAACTTGTTTTCTGGTCTCCATATGCCTTCTTCTGATGGACATTCGATTGTTTCTTAATCAAATTGGTAACCTTTTGTCTTACTTGCTTTTTTCTTCCCTCCTTTTCTTTCCTTCCTTCCTGCTGTTAAGACATAAGCTTTTTATTAATACAAAAGGAGGAGGGATAGACAAGTTATTGAGCCAGTTAGGGACCTAGAGAAATGAAAAGGAATTACAGAAAGCTGTTTTTTCTCTTGTTAGTTGTTATTAAAATGAGTATGTTTACTTAATTAATTTGAGTTACTTTTCTTAAAAACGAGCATGTCAATGTCTTCAGTAAATGTTATTTTTTTGTAATATTTAATTATGAGGTTTGACCTCATGGTGGTTGTACCTTATTTCTCTTGAGCCTGTTCTGCAACTTGTGAGGTATTTGAATCCTTTTGGAACGTTGTTCTTTCTTGCTGGTGTGAACGTGAATAGGAAAACATGATATACCTGCTTTGAATAACGAAAGCCTCCATTCACTTAAAAAAAGAACTTATCAAAAAAAAAAAAAACTTATCATTCACTTAAAAAAAGAAGATGATAAGAAATCCACCCAAAGCTTGTTATTTGTGCCAATTGGAGTGTTTTTTGTGAGCTCCTTGGCCTCGTGAGAGTATTTCCTATTCTCTCCTTTGTATTCTTTCATTTTTAATGAAAGTTTTTTTTTATTATTAAAAAAGTCATAGGCTTACCTAGGATAAGTATTCTTGGCAACCAAATGTGGGGTCTGATGGTTGTCCTGTGAAATTAGTCGAGGTACGTGCAAGCTGGTCGGATACTCACGGATATCTAAAAAAAGCCATAGGCCCATATCTACACTTCTTTTCATACCCTCTAGCTTTATCTTGAATACTTCCTTGGTTTCTTCCCCTCCAAATCTTCCAAAGAATAGCTTTGGCTGCATTTTCCACAAAATTCTAAATTTTTCTTTAAACAACTGACCGGACAATAATAGGATGAGGCCACAGCTTCTGTACCATTTTATGGGAAACACCAAACTCCATAGCTTCTTAGAGAAATACTCCAGCATTTTGATTCAGCAACAATTAATGTGAATTTGTTTCTCCGTTTTCCTCAGCCCTATTACATAATAAGCACAGCACCAATTACGTTAAAAAGCCATATATGGTCATCTTTTTTTTTTCTCCCTCCTCTTTGATTTTACTTTATTTTCTTTCTTCCCTTCCCACCTCTCCCATTTCTATCTACATTTAGTGCAATCTTTTTGAATCTACACTTGAAACTTTCCTCCTAAGAGGTATATTTGTCACGTTGGAATTTGAAGCATCATATATTTTATTTTATTATTTTAGTAGTTTGATGCAGCTCAGTTCTTATTAGATCCGTCACTTGTGACTACTTTTTAGGATGATTCTGAAACTGTTGCTATGATCAAAGAGCTGCTTGACACCGTATTCGACCAGCAGTGCAAGATGATGGTGGAGATATTGAATACAGGGGATATAATGAGTAGGTTTCCCTGGATTTAGTTAAAGGTGTTTCTTAAAGCATTAGGGATTGTATGCATGATTGCTTATATCTACTGAAGTTTGCATTTCTGTCTTCTTCAGAGAAACTGGTATAGTTAGATTGAGAATGCAAGGAGCATGCAGTGGCTGTCCAAGCTCATCTGTCACTCTAAAATCAGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAGGATGATATGATCTTGGTATATTTGTTAGCTCATGTTTAGTGACAAGCATGTCCAATTTTTCACTTGCACAACTAGGTAGGTCCTTTTAAGCATTTTTGGCACAGTTGCTCACGCTATTGCTTTTGTTTCCTGATATTTTTTGCTGGCAGTATCTTTTATATGATACCCGAGGTGTCTTACAAGCATAGTTCTTGTATGCATTTAGTTGCATGCTGCAAACAGCTGGAGTGGAAGGACATTGCATTTATATAATTGAGATTGAATCTTATTGTTATGACTAATAACAGTTGGTTTTCCAACTTCTTTATCCCCCCATAATAATCAACTGCTTATAGGCATCTTATTAACAGAGACTTGTTATAGACAAGATAATTGCTACAGTTTATATTTTACTGAAAATAATCATAAGGAATGCAACATGGTCTAGTTGGATAACAAGAATGCATTGACAAAAGGAGCATGTCTGATAAGAAAAAGGGTATTGCATCCTAGAGAGCCAATTTAGAGAATCACTTAAAGGGAGAAATAGAGTGATGTTTTAGAGCTGCTTAGCCCAATTGTAATAAATAGCTTTTTCACTCCTCTTTTGTTCTTATGCTTCTCTGAATTATATATATATTTTTTATTGATTATCTATGATTTGATAATCTATTCTCAATTTATAGGTGAAGGGCGTGGAACAAGAATTAGATGGTGAGGAAGAGGATGTAACATCAACCAGTCAGATGCAATAAAAGACGACATGGTCGATTCATTCTTTATTAGGTTGAGCCTCCACGCTGATCTTGAGGGTGACCATGCTGTACTTGTTTTTATGCAGAGAATATAAAGGTCATTCGGGTTTAATTATTTCATGGTTGATAGAGTTCCTTGTAATTTTTATTCCTAGTTAGGAAAAGAAGTGTGGATATTGAACCATAAGTGGGATTTCAAGGGCTTAGCATATGTTTGTCGCATTAACTGCAAGATTTTCCACATGACGAACATATGTGATAATAATGTAAGATAGGTTTGATTGTTCATCCAATAATGATATTTTTGTTCTATATCCTGGAATGTTGAAACGATTTCAATTATCTTTAACATATTAAACTTCTGCTGTTGCTGGTAATGTTACCATCTTATAGGATGAGAGATGTGGTAGTAGAAGCCATTCTTCTCTTGGCTGAGAGAGAGAAACTGTAGGTTGAGATTTTCATTTTGATTAAATTTAGGTGGAGTGACACTGCATTAATGCAGAACTTTATTATCAAGTTGCTAATCTTTTTGGCAATGTATGCTAAAAAGCATGGATGTTGTTGCTATTTTATATCTGCATATCCTAAAGTTCATTTGTTGATTTGTGATGATGACTTTTGAATATTTAAATTAAATGTCCCCTTGACCTAATTATTCATGCTGAGTGTACTTTGCATGCTGCAACAGGCAACCAGCCTGTTTCTATAGGCTCTGGACGGACGTGGCAATTAATGTGAGAGGCCTAACGCAACTTCTAATGCCGAAAGAATTTTCAATTTGGTATAGTTGAATATCCTCTTTTGGATTAGAGGAAATGAGTTCAAACCATGTTGACCACTTAGATTTAAAATCCTATGAGTTTTCTTGGCAACTAAATGTTATAGGACCAGACAGTTGTCTCGTAAAATTAGTTGAGATGTACGTAAATTAATTCAAATACTCACAAATTTTTTAAAAAATTGAAGAAATTGAATATAGCACTTGCAATTGGCCAATTCTTTGGATGCCTTGTGGGCACTTTAGTCACTATCAAACCTCTCATCTAGGTTGAATTTTTCAAAGATACCTTTAATAACATAAAGTTACTTTTGTCAGCACAAAGCCCATATAGAATTTTTTTTAATCATTTGAAAATTTGAATATCAATCTCATTAATAGCAAAATATTTTTACCTCTTTTGTAGGTCACTTGCGAATGGGGGAATCTCCCTCCTCCTGGATTTGGAAGCTTTCAACTTTCAAGTCACTTGCCAACAATAAATTCTCCACCGTTGCATATCAGGAAAGATCCATTATTGATAATAACAAAGACCTTCACAAGAGGTAAAAACATGAATTAATTGAGTGCTCTTTTATACAAGTCTTACCTTCCTCCAAATCAAATCACTTAAATTAGAGATGTCTTTTGTCTCTAAGTTTTTTAGCAAAGCATAATGAAAATTGTTAAATTTATGTCACTAAAATTAAAATCAAGTCCAACTTTCTATTATTGTTTAAAAAAATTTGGAATGGAAGTGGCCATCAAGGTTACGCATTAGTTGTGGATATGACATATGACATATGAGAGCATGCTTTAGACTTTTATTATCAACATCTTGGTGGTGAAAGAGAAGGCATCGTAATCGAACCTAAAAAAGCGAAGCCACGCGCACTCAATGCAACGCCACACGCCTTCCGCGCGCCAACTCGTGCGCGCGCATGTCCTGCCTAGCCGCCAGATCCCACGCACACACTCACAACTTCCTTCTACATCCTCAAAATTTCATATATTATTTCTTTTGTTCAATTCCAGGTTTTGGTCTTAAACTTTTGGCTAAATATATATTTTAATCTTTAATATCTGAGTACTTTTTTTTTTAATTTAGTCTATAATATTTTAATATTTTTTAATTACACCCTTCTTGTTGATAAATATTAAAATTGGCTAATGATAAACTTACATGACATAATACTCAGTGAGTTGAAAAAAAAATTAGAAGATAAATTAAGTTATCTAAAAGAGTGCAAGTTTCTAATTTTTCCTCCAAATTTTCAAGTTGGCAAAAATTTCGACTTTCTTTTCTTAGGTGCCTAATTATTCCTCTAAATGTCTACAAACTCACTAAGTATCATATCATGTAAGCTTACCATCAGCCAATTTTAACATTCACGATGAAATAATATAATTGAAAAATGTCAAAACATTATGGACTAAATTGAAAAAATATATAAATATTAGGGACTAAATCATCTATTTTACGTAAACTTTTAATAAGGGTGTTTATTTCATTCATTTCTGATAGGTTCGTAAATTTTGAATGTTGTATTTATTTAGTCTCCATACTTTAAAAATAGACTTTAAATTTTTAATTTTGTGTCTAATAAACTTTATCTTTAATTTTATTAGTTTAACGTTTGTCAATAAATGCTTGAAACTACTTGAAATCTGTTTCTCACGTAATTTTAAATAATACAGTTAATACATCACATAGATATTAGACTACAAACTTAACGATGGATACTAATTAAACAAAAATTGAATGTTTAAAAACTTATTAAAAACTTTTTCAAAGTAGAAATACTAAATAGACATGATAAATTAAGAGACCTATTAAAAATATTTAAAATTTAAGAGATTAAACAATACAACTTTAAAAGTTACGTGTACAAACTTGTAATTTAACTTTTTTTTCTCATTATTAGCGACTCCTAAATCTTGCAACGGACATTGTTGTTCATAAATTACAGCGTGGAGACACGCGCCCTTGTTTGCATATAGCATCAGCCATCTTGAATTTAATTGAACCATCAACTTCCACCAACCTCTCTCTCTGTCTTTTCCCCTAAAATTCAGAACGTGTATCAGCTTTGTCTTCACCTCAATCATCAAATCATTACCTATCTCTTTCCCTCATCTATAAACTTGGGTTATCACTTGGTTTCGTTTCCGACAGCCGTTTTATCTGGTTTCAGAACAATACAGACAACAGCGTTTTGTCTGTCTTTGTCTGAAGAAGCACCTGACGTATATTTATATATTTTGACTTGTTTTCATAAATACCAAGACTTGCAAGGTTTTGGCTTTGGAGATGCAGTACGCTGCCTGGAAATGTCGGTTTGGAGAGCTGAGGCCACTGGTTCACTTGTTGCTGCCACTATGTGTCCACTGGATCGCTGAAGAAATGACTGTCTCTGTTCTTGTTGATATCATTACTAATGCTCTCTGTCCTGGGAATCCCACTTGCCCTCAAGCAATTTACTTCAATGGAACTGAGCAAACC

mRNA sequence

ATGAGGGGCTTAGGGAGCTTACTTGGGCGAGCTCGGAGGATACCTAGATTGGATTCTCGCGGAGGCGGTAAGATCCGGCGCCTCTCCGCCGCAACAACTTCTTCTCTGTTCGGCGGCGAAGACGCTGCTTCGTGTTCTGCGTCGTCTAGGTCGTCTTCTTCATTCACTTCTTCTTTGCGCTCTTGGAAATGGAACCACTTCAAAGGACAGAGGAGAACAATGTTTATTCAAACTCAATCAACACCCAATCCTTCGTCTCTCATGTTCCATCCTGGAAAGCCTGTTATGGAAGTTGGGAGTGCAGATTTTCCGAATGCGCGTTCTGCTATGAACTCCCCGCTAGCGAAAGCCCTTTATGGCATTGATGGTATTATCCGAGTCTTCTTTGGATCGGATTTTGTTACTGTAACAAAATCAGAAGATGCTTCTTGGGATTTTTTAAAGCCTGAAATATTTGCCGCAATCATGGACTTCTATTCTTCTGGGCAACCACTATTTCTGGACTCTAAAACTGCTGCCGCCATGGACACAGCCATCAATGAAGATGATTCTGAAACTGTTGCTATGATCAAAGAGCTGCTTGACACCGTATTCGACCAGCAGTGCAAGATGATGGTGGAGATATTGAATACAGGGGATATAATGAGTAGAGAAACTGGTATAGTTAGATTGAGAATGCAAGGAGCATGCAGTGGCTGTCCAAGCTCATCTGTCACTCTAAAATCAGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAAGGGCGTGGAACAAGAATTAGATGGTGAGGAAGAGGATGTAACATCAACCACCGTTTTATCTGGTTTCAGAACAATACAGACAACAGCGTTTTGTCTGTCTTTGTCTGAAGAAGCACCTGACGTTTTGGCTTTGGAGATGCAGTACGCTGCCTGGAAATGTCGGTTTGGAGAGCTGAGGCCACTGGTTCACTTGTTGCTGCCACTATGTGTCCACTGGATCGCTGAAGAAATGACTGTCTCTGTTCTTGTTGATATCATTACTAATGCTCTCTGTCCTGGGAATCCCACTTGCCCTCAAGCAATTTACTTCAATGGAACTGAGCAAACC

Coding sequence (CDS)

ATGAGGGGCTTAGGGAGCTTACTTGGGCGAGCTCGGAGGATACCTAGATTGGATTCTCGCGGAGGCGGTAAGATCCGGCGCCTCTCCGCCGCAACAACTTCTTCTCTGTTCGGCGGCGAAGACGCTGCTTCGTGTTCTGCGTCGTCTAGGTCGTCTTCTTCATTCACTTCTTCTTTGCGCTCTTGGAAATGGAACCACTTCAAAGGACAGAGGAGAACAATGTTTATTCAAACTCAATCAACACCCAATCCTTCGTCTCTCATGTTCCATCCTGGAAAGCCTGTTATGGAAGTTGGGAGTGCAGATTTTCCGAATGCGCGTTCTGCTATGAACTCCCCGCTAGCGAAAGCCCTTTATGGCATTGATGGTATTATCCGAGTCTTCTTTGGATCGGATTTTGTTACTGTAACAAAATCAGAAGATGCTTCTTGGGATTTTTTAAAGCCTGAAATATTTGCCGCAATCATGGACTTCTATTCTTCTGGGCAACCACTATTTCTGGACTCTAAAACTGCTGCCGCCATGGACACAGCCATCAATGAAGATGATTCTGAAACTGTTGCTATGATCAAAGAGCTGCTTGACACCGTATTCGACCAGCAGTGCAAGATGATGGTGGAGATATTGAATACAGGGGATATAATGAGTAGAGAAACTGGTATAGTTAGATTGAGAATGCAAGGAGCATGCAGTGGCTGTCCAAGCTCATCTGTCACTCTAAAATCAGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAAGGGCGTGGAACAAGAATTAGATGGTGAGGAAGAGGATGTAACATCAACCACCGTTTTATCTGGTTTCAGAACAATACAGACAACAGCGTTTTGTCTGTCTTTGTCTGAAGAAGCACCTGACGTTTTGGCTTTGGAGATGCAGTACGCTGCCTGGAAATGTCGGTTTGGAGAGCTGAGGCCACTGGTTCACTTGTTGCTGCCACTATGTGTCCACTGGATCGCTGAAGAAATGACTGTCTCTGTTCTTGTTGATATCATTACTAATGCTCTCTGTCCTGGGAATCCCACTTGCCCTCAAGCAATTTACTTCAATGGAACTGAGCAAACC

Protein sequence

MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLRSWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSRETGIVRLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTTVLSGFRTIQTTAFCLSLSEEAPDVLALEMQYAAWKCRFGELRPLVHLLLPLCVHWIAEEMTVSVLVDIITNALCPGNPTCPQAIYFNGTEQT
Homology
BLAST of Sgr022642 vs. NCBI nr
Match: XP_022133710.1 (nifU-like protein 4, mitochondrial [Momordica charantia])

HSP 1 Score: 424.5 bits (1090), Expect = 9.4e-115
Identity = 230/277 (83.03%), Postives = 244/277 (88.09%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLGSL+ R RRIPR+DSRG GKIRR+S ATTSSLFGGEDAASCS +SRS S+  S LR
Sbjct: 1   MRGLGSLIVRVRRIPRMDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSN--SYLR 60

Query: 61  SWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120
           SWKWN F GQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG
Sbjct: 61  SWKWNQFGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120

Query: 121 IDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180
           IDG++RVFFGSDFVTVTKSEDA+WDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN
Sbjct: 121 IDGVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180

Query: 181 EDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPSS 240
           EDDSE VAMIKELL+T    + +  V+  + GDI  R    ETGIV LRMQGACSGCPSS
Sbjct: 181 EDDSEIVAMIKELLET----RIRPAVQD-DGGDIEYRGYNEETGIVTLRMQGACSGCPSS 240

Query: 241 SVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SVTLKSGIENMLMHYVPEVKGVEQE D EEED  ST+
Sbjct: 241 SVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDAISTS 270

BLAST of Sgr022642 vs. NCBI nr
Match: XP_022960817.1 (nifU-like protein 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 403.3 bits (1035), Expect = 2.2e-108
Identity = 221/277 (79.78%), Postives = 242/277 (87.36%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLGSL+ RARRIP L+SRG GKIR +SAA TSSLF GE+AAS S SSR+ S   S LR
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSD--SYLR 60

Query: 61  SWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120
           SWKWN F GQRRTMFI+TQSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLA+ALYG
Sbjct: 61  SWKWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYG 120

Query: 121 IDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180
           +DG++RVFFGSDFVTVTKS DASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAIN
Sbjct: 121 VDGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAIN 180

Query: 181 EDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPSS 240
           EDDSETVAMIKELL+T    + +  V+  + GDI  R    ETGIV+LRMQGACSGCPSS
Sbjct: 181 EDDSETVAMIKELLET----RIRPAVQD-DGGDIEYRGYDEETGIVKLRMQGACSGCPSS 240

Query: 241 SVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SVTLKSGIENMLMHYVPEVKGVEQE D EE+D+ ST+
Sbjct: 241 SVTLKSGIENMLMHYVPEVKGVEQEFDVEEDDIKSTS 270

BLAST of Sgr022642 vs. NCBI nr
Match: XP_038884119.1 (nifU-like protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 401.4 bits (1030), Expect = 8.5e-108
Identity = 221/277 (79.78%), Postives = 239/277 (86.28%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLG L+ RARRIP +   GGGK R  SAATTSS+FGGE+AASCS SSR+ S+  S LR
Sbjct: 1   MRGLGRLILRARRIPLMAFHGGGKTRFFSAATTSSMFGGEEAASCSISSRNCSN--SYLR 60

Query: 61  SWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120
           SWK N F GQRRTMFIQTQSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLAKALYG
Sbjct: 61  SWKSNQFGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYG 120

Query: 121 IDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180
           +DG++RVFFGSDFVTVTKS DASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAI 
Sbjct: 121 VDGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIK 180

Query: 181 EDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPSS 240
           EDDSETVAMIKELL+T    + +  V+  + GDI  R    ETGIV+LRMQGACSGCPSS
Sbjct: 181 EDDSETVAMIKELLET----RIRPAVQD-DGGDIEYRGYNEETGIVKLRMQGACSGCPSS 240

Query: 241 SVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SVTLKSGIENMLMHYVPEVKGVEQE D +EEDV ST+
Sbjct: 241 SVTLKSGIENMLMHYVPEVKGVEQEFDLDEEDVKSTS 270

BLAST of Sgr022642 vs. NCBI nr
Match: XP_022992592.1 (nifU-like protein 4, mitochondrial [Cucurbita maxima])

HSP 1 Score: 399.4 bits (1025), Expect = 3.2e-107
Identity = 217/277 (78.34%), Postives = 239/277 (86.28%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLGSL+ RARRI  L S GGGKIR  SAATTSS+FGG++AASCS SSR+ S     LR
Sbjct: 1   MRGLGSLIVRARRISTLGSHGGGKIRFFSAATTSSIFGGKEAASCSVSSRTCSD--PFLR 60

Query: 61  SWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120
           SW W+ F GQ+RTMFI+TQSTPNPSSLMF+PGKPVMEVGSADFPNAR+AMNSPLAKALYG
Sbjct: 61  SWSWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARTAMNSPLAKALYG 120

Query: 121 IDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180
           +DG+IRVFFGSDFVTVTKS +ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAIN
Sbjct: 121 VDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAIN 180

Query: 181 EDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPSS 240
           EDDSETVAMIKELL+T    + +  V+  + GDI  R    ETGIV+LRMQGACSGCPSS
Sbjct: 181 EDDSETVAMIKELLET----RIRPTVQD-DGGDIEYRGYNEETGIVKLRMQGACSGCPSS 240

Query: 241 SVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SVTLKSGIENMLMHYVPEVKGVEQE D EEEDV ST+
Sbjct: 241 SVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDVKSTS 270

BLAST of Sgr022642 vs. NCBI nr
Match: XP_023517879.1 (nifU-like protein 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399.1 bits (1024), Expect = 4.2e-107
Identity = 222/278 (79.86%), Postives = 242/278 (87.05%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLGSL+ RARRIP L+SRG GKIR +SAA TSSLF GE+AAS S SSR+ S   S LR
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSD--SYLR 60

Query: 61  SWKWNH-FKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALY 120
           SWKWN  F GQRRTMFI+TQSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLA+ALY
Sbjct: 61  SWKWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALY 120

Query: 121 GIDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAI 180
           G+DG++RVFFGSDFVTVTKS DASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAI
Sbjct: 121 GVDGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAI 180

Query: 181 NEDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPS 240
           NEDDSETVAMIKELL+T    + +  V+  + GDI  R    ETGIV+LRMQGACSGCPS
Sbjct: 181 NEDDSETVAMIKELLET----RIRPAVQD-DGGDIEYRGYDEETGIVKLRMQGACSGCPS 240

Query: 241 SSVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SSVTLKSGIENMLMHYVPEVKGVEQE D EE+DV ST+
Sbjct: 241 SSVTLKSGIENMLMHYVPEVKGVEQEFDVEEDDVKSTS 271

BLAST of Sgr022642 vs. ExPASy Swiss-Prot
Match: Q9LIG6 (NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.6e-85
Identity = 180/278 (64.75%), Postives = 212/278 (76.26%), Query Frame = 0

Query: 9   GRARRIPRLDSRGGGKI----RRLSAATTSSLFGGEDAASCSASSRSSS--------SFT 68
           G AR +  L   GG K+      ++++++SSL     +   SA++  +S        S  
Sbjct: 3   GIARLVTSLSRIGGRKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSALPSLN 62

Query: 69  SSLRSWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAK 128
           SSL + KWN   GQRRTMFIQTQSTPNPSSLMF+PGKPVMEVGSADFPN RSA+ SPLAK
Sbjct: 63  SSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGSPLAK 122

Query: 129 ALYGIDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMD 188
           ++Y IDG++RVFFGSDFVTVTKS+D SWD LKPEIFAA+MDFYSSGQPLFLDS+ AAA D
Sbjct: 123 SIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAAAAKD 182

Query: 189 TAINEDDSETVAMIKELLDTVFDQQCKMMVEILNTGDI----MSRETGIVRLRMQGACSG 248
           TAI+EDDSETVAMIKELL+T    + +  V+  + GDI       E+GIV+LRMQGACSG
Sbjct: 183 TAISEDDSETVAMIKELLET----RIRPAVQD-DGGDIEYCGFDPESGIVKLRMQGACSG 242

Query: 249 CPSSSVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVT 271
           CPSSSVTLKSGIENMLMHYV EVKGVEQE DGE+E+ T
Sbjct: 243 CPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEDEEGT 275

BLAST of Sgr022642 vs. ExPASy Swiss-Prot
Match: Q9C8J2 (NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 3.0e-79
Identity = 166/248 (66.94%), Postives = 196/248 (79.03%), Query Frame = 0

Query: 28  LSAATTSSLFGGEDAASCSASSRSSSSFTSSLRSWKWNHFKGQRRTMFIQTQSTPNPSSL 87
           +SAAT   +   E   + ++ + S SS      S+ +N  + QRRTMFIQTQSTPNPSSL
Sbjct: 36  ISAATHFPVNFSESITTNASRNCSRSS------SFPFNWIE-QRRTMFIQTQSTPNPSSL 95

Query: 88  MFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGIIRVFFGSDFVTVTKSEDASWDFL 147
           MF PGKPVME+GSADFPN+RSAM+SPLAKA++ IDG++RVF+GSDFVTVTKS+D +WD L
Sbjct: 96  MFSPGKPVMEIGSADFPNSRSAMSSPLAKAIFAIDGVVRVFYGSDFVTVTKSDDVTWDIL 155

Query: 148 KPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLDTVFDQQCKMMVE 207
           KP+IFA +MDFYSSGQPLFLDS+  AA DTAI+EDDSETVAMIKELL+T    + +  V+
Sbjct: 156 KPDIFAVVMDFYSSGQPLFLDSQATAAKDTAIHEDDSETVAMIKELLET----RIRPSVQ 215

Query: 208 ILNTGDI----MSRETGIVRLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQELD 267
             + GDI       ETGIV+LRMQGACSGCPSSSVTLKSGIENMLMHYV EVKGVEQE D
Sbjct: 216 D-DGGDIEYCGFDTETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFD 271

Query: 268 GEEEDVTS 272
           GEEE  +S
Sbjct: 276 GEEEGTSS 271

BLAST of Sgr022642 vs. ExPASy Swiss-Prot
Match: Q8SY96 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=CG32500 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.0e-50
Identity = 110/204 (53.92%), Postives = 143/204 (70.10%), Query Frame = 0

Query: 71  RRTMFIQTQSTPNPSSLMFHPGKPVMEVGSA-DFPNARSAMNSPLAKALYGIDGIIRVFF 130
           RR+MFIQTQ TPNP SL F PG  V+  G+  DFPN  +A NSPLAK L+ ++G+  VFF
Sbjct: 64  RRSMFIQTQDTPNPESLKFLPGVDVLGKGNTYDFPNGTTAHNSPLAKLLFRVEGVKGVFF 123

Query: 131 GSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAM 190
           G+DFVT++K E A W  +KPE+FA IMDF++SG P+  D++  A  DT I EDD ETV M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLNDAQPNA--DTEILEDDDETVMM 183

Query: 191 IKELLDTVFDQQCKMMVEILNTGDI--MSRETGIVRLRMQGACSGCPSSSVTLKSGIENM 250
           IKELLDT    + +  V+  + GDI  M  E G+V+L+MQG+CS CPSS VTLK+G++NM
Sbjct: 184 IKELLDT----RIRPTVQ-EDGGDIVFMGYEGGVVKLKMQGSCSSCPSSIVTLKNGVQNM 243

Query: 251 LMHYVPEVKGVEQELDGEEEDVTS 272
           L  Y+PEV+ VEQ  D  +  + S
Sbjct: 244 LQFYIPEVESVEQVFDEADRMIES 260

BLAST of Sgr022642 vs. ExPASy Swiss-Prot
Match: B4PZ52 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila yakuba OX=7245 GN=GE15286 PE=3 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.7e-50
Identity = 110/204 (53.92%), Postives = 143/204 (70.10%), Query Frame = 0

Query: 71  RRTMFIQTQSTPNPSSLMFHPGKPVMEVGSA-DFPNARSAMNSPLAKALYGIDGIIRVFF 130
           RR+MFIQTQ TPNP SL F PG  V+  G+  DFPN  +A +SPLAK L+ ++G+  VFF
Sbjct: 64  RRSMFIQTQDTPNPDSLKFLPGVDVLGKGNTYDFPNGTTAHSSPLAKLLFRVEGVKGVFF 123

Query: 131 GSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAM 190
           G+DFVT++K E A W  +KPE+FA IMDF++SG P+  D++  A  DT I EDD ETV M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLHDAQPNA--DTEILEDDDETVMM 183

Query: 191 IKELLDTVFDQQCKMMVEILNTGDI--MSRETGIVRLRMQGACSGCPSSSVTLKSGIENM 250
           IKELLDT    + +  V+  + GDI  M  E G+V+L+MQG+CS CPSS VTLK+G++NM
Sbjct: 184 IKELLDT----RIRPTVQ-EDGGDIVFMGYEAGVVKLKMQGSCSSCPSSIVTLKNGVQNM 243

Query: 251 LMHYVPEVKGVEQELDGEEEDVTS 272
           L  Y+PEV+ VEQ  D  +  V S
Sbjct: 244 LQFYIPEVESVEQVFDEADRMVDS 260

BLAST of Sgr022642 vs. ExPASy Swiss-Prot
Match: Q9QZ23 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Mus musculus OX=10090 GN=Nfu1 PE=1 SV=2)

HSP 1 Score: 201.4 bits (511), Expect = 1.7e-50
Identity = 108/200 (54.00%), Postives = 144/200 (72.00%), Query Frame = 0

Query: 72  RTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGIIRVFFGS 131
           R MFIQTQ TPNP+SL F PGKPV+E  + DFP   +A  SPLA+ L+ I+G+  VFFG 
Sbjct: 55  RFMFIQTQDTPNPNSLKFIPGKPVLETRTMDFPTPAAAFRSPLARQLFRIEGVKSVFFGP 114

Query: 132 DFVTVTK-SEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAMI 191
           DF+TVTK +E+  W+ LKP+I+A IMDF++SG PL +  +T      A +E+D E VAMI
Sbjct: 115 DFITVTKENEELDWNLLKPDIYATIMDFFASGLPL-VTEETPPPPGEAGSEEDDEVVAMI 174

Query: 192 KELLDTVFDQQCKMMVEILNTGDIMSR--ETGIVRLRMQGACSGCPSSSVTLKSGIENML 251
           KELLDT    + +  V+  + GD++ R  E GIVRL++QG+C+ CPSS +TLKSGI+NML
Sbjct: 175 KELLDT----RIRPTVQ-EDGGDVIYRGFEDGIVRLKLQGSCTSCPSSIITLKSGIQNML 234

Query: 252 MHYVPEVKGVEQELDGEEED 269
             Y+PEV+GVEQ +D +E D
Sbjct: 235 QFYIPEVEGVEQVMDDDESD 248

BLAST of Sgr022642 vs. ExPASy TrEMBL
Match: A0A6J1BW08 (nifU-like protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111006227 PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 4.6e-115
Identity = 230/277 (83.03%), Postives = 244/277 (88.09%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLGSL+ R RRIPR+DSRG GKIRR+S ATTSSLFGGEDAASCS +SRS S+  S LR
Sbjct: 1   MRGLGSLIVRVRRIPRMDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSN--SYLR 60

Query: 61  SWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120
           SWKWN F GQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG
Sbjct: 61  SWKWNQFGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120

Query: 121 IDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180
           IDG++RVFFGSDFVTVTKSEDA+WDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN
Sbjct: 121 IDGVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180

Query: 181 EDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPSS 240
           EDDSE VAMIKELL+T    + +  V+  + GDI  R    ETGIV LRMQGACSGCPSS
Sbjct: 181 EDDSEIVAMIKELLET----RIRPAVQD-DGGDIEYRGYNEETGIVTLRMQGACSGCPSS 240

Query: 241 SVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SVTLKSGIENMLMHYVPEVKGVEQE D EEED  ST+
Sbjct: 241 SVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDAISTS 270

BLAST of Sgr022642 vs. ExPASy TrEMBL
Match: A0A6J1H8H2 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 1.1e-108
Identity = 221/277 (79.78%), Postives = 242/277 (87.36%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLGSL+ RARRIP L+SRG GKIR +SAA TSSLF GE+AAS S SSR+ S   S LR
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSD--SYLR 60

Query: 61  SWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120
           SWKWN F GQRRTMFI+TQSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLA+ALYG
Sbjct: 61  SWKWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYG 120

Query: 121 IDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180
           +DG++RVFFGSDFVTVTKS DASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAIN
Sbjct: 121 VDGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAIN 180

Query: 181 EDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPSS 240
           EDDSETVAMIKELL+T    + +  V+  + GDI  R    ETGIV+LRMQGACSGCPSS
Sbjct: 181 EDDSETVAMIKELLET----RIRPAVQD-DGGDIEYRGYDEETGIVKLRMQGACSGCPSS 240

Query: 241 SVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SVTLKSGIENMLMHYVPEVKGVEQE D EE+D+ ST+
Sbjct: 241 SVTLKSGIENMLMHYVPEVKGVEQEFDVEEDDIKSTS 270

BLAST of Sgr022642 vs. ExPASy TrEMBL
Match: A0A6J1JQB1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.6e-107
Identity = 217/277 (78.34%), Postives = 239/277 (86.28%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLGSL+ RARRI  L S GGGKIR  SAATTSS+FGG++AASCS SSR+ S     LR
Sbjct: 1   MRGLGSLIVRARRISTLGSHGGGKIRFFSAATTSSIFGGKEAASCSVSSRTCSD--PFLR 60

Query: 61  SWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120
           SW W+ F GQ+RTMFI+TQSTPNPSSLMF+PGKPVMEVGSADFPNAR+AMNSPLAKALYG
Sbjct: 61  SWSWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARTAMNSPLAKALYG 120

Query: 121 IDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180
           +DG+IRVFFGSDFVTVTKS +ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAIN
Sbjct: 121 VDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAIN 180

Query: 181 EDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPSS 240
           EDDSETVAMIKELL+T    + +  V+  + GDI  R    ETGIV+LRMQGACSGCPSS
Sbjct: 181 EDDSETVAMIKELLET----RIRPTVQD-DGGDIEYRGYNEETGIVKLRMQGACSGCPSS 240

Query: 241 SVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SVTLKSGIENMLMHYVPEVKGVEQE D EEEDV ST+
Sbjct: 241 SVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDVKSTS 270

BLAST of Sgr022642 vs. ExPASy TrEMBL
Match: A0A6J1FIB0 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 3.5e-107
Identity = 217/277 (78.34%), Postives = 238/277 (85.92%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLGSL+ RARRI  L S G GKIR  SAATTSS+FGG +AASCS SSR  + F   LR
Sbjct: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGNEAASCSVSSR--TCFDPLLR 60

Query: 61  SWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120
           SW+W+ F GQ+RTMFI+TQSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLAKALYG
Sbjct: 61  SWRWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYG 120

Query: 121 IDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180
           +DG+IRVFFGSDFVTVTKS +ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAIN
Sbjct: 121 VDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAIN 180

Query: 181 EDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPSS 240
           EDDSETVAMIKELL+T    + +  V+  + GDI  R    ETGIV+LRMQGACSGCPSS
Sbjct: 181 EDDSETVAMIKELLET----RIRPTVQD-DGGDIEYRGYNEETGIVKLRMQGACSGCPSS 240

Query: 241 SVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SVTLKSGIENMLMHYVPEVKGVEQE D EEEDV ST+
Sbjct: 241 SVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDVKSTS 270

BLAST of Sgr022642 vs. ExPASy TrEMBL
Match: A0A6J1JTA1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 PE=3 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.7e-106
Identity = 219/277 (79.06%), Postives = 238/277 (85.92%), Query Frame = 0

Query: 1   MRGLGSLLGRARRIPRLDSRGGGKIRRLSAATTSSLFGGEDAASCSASSRSSSSFTSSLR 60
           MRGLGSL+ RARRIP L+ RG GKIR +SAA TSSLF GE+AAS S SSR+ S   S LR
Sbjct: 1   MRGLGSLIVRARRIPGLNPRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTCSD--SYLR 60

Query: 61  SWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYG 120
           SWKWN F GQRRTMFI+TQSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLA+ALYG
Sbjct: 61  SWKWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYG 120

Query: 121 IDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIN 180
           +DG++RVFFGSDFVTV KS DASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAIN
Sbjct: 121 VDGVVRVFFGSDFVTVRKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAIN 180

Query: 181 EDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSR----ETGIVRLRMQGACSGCPSS 240
           EDDSETVAMIKELL+T    + +  V+  + GDI  R    ETGIV LRMQGACSGCPSS
Sbjct: 181 EDDSETVAMIKELLET----RIRPAVQD-DGGDIEYRGYDEETGIVNLRMQGACSGCPSS 240

Query: 241 SVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVTSTT 274
           SVTLKSGIENMLMHYVPEVKGVEQ  D EE+DV ST+
Sbjct: 241 SVTLKSGIENMLMHYVPEVKGVEQVFDIEEDDVKSTS 270

BLAST of Sgr022642 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 316.6 bits (810), Expect = 2.6e-86
Identity = 180/278 (64.75%), Postives = 212/278 (76.26%), Query Frame = 0

Query: 9   GRARRIPRLDSRGGGKI----RRLSAATTSSLFGGEDAASCSASSRSSS--------SFT 68
           G AR +  L   GG K+      ++++++SSL     +   SA++  +S        S  
Sbjct: 3   GIARLVTSLSRIGGRKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSALPSLN 62

Query: 69  SSLRSWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAK 128
           SSL + KWN   GQRRTMFIQTQSTPNPSSLMF+PGKPVMEVGSADFPN RSA+ SPLAK
Sbjct: 63  SSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGSPLAK 122

Query: 129 ALYGIDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMD 188
           ++Y IDG++RVFFGSDFVTVTKS+D SWD LKPEIFAA+MDFYSSGQPLFLDS+ AAA D
Sbjct: 123 SIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAAAAKD 182

Query: 189 TAINEDDSETVAMIKELLDTVFDQQCKMMVEILNTGDI----MSRETGIVRLRMQGACSG 248
           TAI+EDDSETVAMIKELL+T    + +  V+  + GDI       E+GIV+LRMQGACSG
Sbjct: 183 TAISEDDSETVAMIKELLET----RIRPAVQD-DGGDIEYCGFDPESGIVKLRMQGACSG 242

Query: 249 CPSSSVTLKSGIENMLMHYVPEVKGVEQELDGEEEDVT 271
           CPSSSVTLKSGIENMLMHYV EVKGVEQE DGE+E+ T
Sbjct: 243 CPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEDEEGT 275

BLAST of Sgr022642 vs. TAIR 10
Match: AT1G51390.1 (NFU domain protein 5 )

HSP 1 Score: 297.0 bits (759), Expect = 2.1e-80
Identity = 166/248 (66.94%), Postives = 196/248 (79.03%), Query Frame = 0

Query: 28  LSAATTSSLFGGEDAASCSASSRSSSSFTSSLRSWKWNHFKGQRRTMFIQTQSTPNPSSL 87
           +SAAT   +   E   + ++ + S SS      S+ +N  + QRRTMFIQTQSTPNPSSL
Sbjct: 36  ISAATHFPVNFSESITTNASRNCSRSS------SFPFNWIE-QRRTMFIQTQSTPNPSSL 95

Query: 88  MFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGIIRVFFGSDFVTVTKSEDASWDFL 147
           MF PGKPVME+GSADFPN+RSAM+SPLAKA++ IDG++RVF+GSDFVTVTKS+D +WD L
Sbjct: 96  MFSPGKPVMEIGSADFPNSRSAMSSPLAKAIFAIDGVVRVFYGSDFVTVTKSDDVTWDIL 155

Query: 148 KPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLDTVFDQQCKMMVE 207
           KP+IFA +MDFYSSGQPLFLDS+  AA DTAI+EDDSETVAMIKELL+T    + +  V+
Sbjct: 156 KPDIFAVVMDFYSSGQPLFLDSQATAAKDTAIHEDDSETVAMIKELLET----RIRPSVQ 215

Query: 208 ILNTGDI----MSRETGIVRLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQELD 267
             + GDI       ETGIV+LRMQGACSGCPSSSVTLKSGIENMLMHYV EVKGVEQE D
Sbjct: 216 D-DGGDIEYCGFDTETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFD 271

Query: 268 GEEEDVTS 272
           GEEE  +S
Sbjct: 276 GEEEGTSS 271

BLAST of Sgr022642 vs. TAIR 10
Match: AT3G20970.2 (NFU domain protein 4 )

HSP 1 Score: 235.3 bits (599), Expect = 7.5e-62
Identity = 130/200 (65.00%), Postives = 154/200 (77.00%), Query Frame = 0

Query: 9   GRARRIPRLDSRGGGKI----RRLSAATTSSLFGGEDAASCSASSRSSS--------SFT 68
           G AR +  L   GG K+      ++++++SSL     +   SA++  +S        S  
Sbjct: 3   GIARLVTSLSRIGGRKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSALPSLN 62

Query: 69  SSLRSWKWNHFKGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAK 128
           SSL + KWN   GQRRTMFIQTQSTPNPSSLMF+PGKPVMEVGSADFPN RSA+ SPLAK
Sbjct: 63  SSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGSPLAK 122

Query: 129 ALYGIDGIIRVFFGSDFVTVTKSEDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMD 188
           ++Y IDG++RVFFGSDFVTVTKS+D SWD LKPEIFAA+MDFYSSGQPLFLDS+ AAA D
Sbjct: 123 SIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAAAAKD 182

Query: 189 TAINEDDSETVAMIKELLDT 197
           TAI+EDDSETVAMIKELL+T
Sbjct: 183 TAISEDDSETVAMIKELLET 202

BLAST of Sgr022642 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 56.2 bits (134), Expect = 6.3e-08
Identity = 36/107 (33.64%), Postives = 57/107 (53.27%), Query Frame = 0

Query: 159 YSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSRE 218
           + S + LF  SK   A+ T    D    V + +E +++V D+    ++       +   +
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVALHEID 117

Query: 219 TGIVRLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQELDGE 266
             IVR+++QGAC  CPSS++T+K GIE  LM  +PE+  VE   D E
Sbjct: 118 GNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEE 161

BLAST of Sgr022642 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 56.2 bits (134), Expect = 6.3e-08
Identity = 36/107 (33.64%), Postives = 57/107 (53.27%), Query Frame = 0

Query: 159 YSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLDTVFDQQCKMMVEILNTGDIMSRE 218
           + S + LF  SK   A+ T    D    V + +E +++V D+    ++       +   +
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVALHEID 117

Query: 219 TGIVRLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQELDGE 266
             IVR+++QGAC  CPSS++T+K GIE  LM  +PE+  VE   D E
Sbjct: 118 GNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEE 161

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133710.19.4e-11583.03nifU-like protein 4, mitochondrial [Momordica charantia][more]
XP_022960817.12.2e-10879.78nifU-like protein 4, mitochondrial [Cucurbita moschata][more]
XP_038884119.18.5e-10879.78nifU-like protein 4, mitochondrial [Benincasa hispida][more]
XP_022992592.13.2e-10778.34nifU-like protein 4, mitochondrial [Cucurbita maxima][more]
XP_023517879.14.2e-10779.86nifU-like protein 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LIG63.6e-8564.75NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2... [more]
Q9C8J23.0e-7966.94NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2... [more]
Q8SY961.0e-5053.92NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogas... [more]
B4PZ521.7e-5053.92NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila yakuba OX... [more]
Q9QZ231.7e-5054.00NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Mus musculus OX=1009... [more]
Match NameE-valueIdentityDescription
A0A6J1BW084.6e-11583.03nifU-like protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC11100622... [more]
A0A6J1H8H21.1e-10879.78nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505... [more]
A0A6J1JQB11.6e-10778.34nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 P... [more]
A0A6J1FIB03.5e-10778.34nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668... [more]
A0A6J1JTA11.7e-10679.06nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 P... [more]
Match NameE-valueIdentityDescription
AT3G20970.12.6e-8664.75NFU domain protein 4 [more]
AT1G51390.12.1e-8066.94NFU domain protein 5 [more]
AT3G20970.27.5e-6265.00NFU domain protein 4 [more]
AT5G49940.16.3e-0833.64NIFU-like protein 2 [more]
AT5G49940.26.3e-0833.64NIFU-like protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014824Scaffold protein Nfu/NifU, N-terminalSMARTSM00932Nfu_N_3acoord: 76..162
e-value: 4.1E-44
score: 162.5
IPR014824Scaffold protein Nfu/NifU, N-terminalPFAMPF08712Nfu_Ncoord: 76..162
e-value: 5.6E-29
score: 100.1
IPR036498Scaffold protein Nfu/NifU, N-terminal domain superfamilyGENE3D3.30.1370.70coord: 64..168
e-value: 8.8E-37
score: 127.0
IPR036498Scaffold protein Nfu/NifU, N-terminal domain superfamilySUPERFAMILY110836Hypothetical protein SAV1430coord: 74..164
IPR001075NIF system FeS cluster assembly, NifU, C-terminalPFAMPF01106NifUcoord: 217..259
e-value: 8.9E-17
score: 61.0
IPR034904Fe-S cluster assembly domain superfamilyGENE3D3.30.300.130coord: 184..260
e-value: 1.3E-20
score: 75.2
IPR034904Fe-S cluster assembly domain superfamilySUPERFAMILY117916Fe-S cluster assembly (FSCA) domain-likecoord: 176..264
NoneNo IPR availablePANTHERPTHR11178IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATEDcoord: 29..272
NoneNo IPR availablePANTHERPTHR11178:SF43NIFU-LIKE PROTEIN 5, MITOCHONDRIALcoord: 29..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr022642.1Sgr022642.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0097428 protein maturation by iron-sulfur cluster transfer
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding