Sgr022489 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr022489 vs. NCBI nr
Match: CAB4289961.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 837.0 bits (2161), Expect = 1.9e-238
Identity = 493/894 (55.15%), Postives = 579/894 (64.77%), Query Frame = 0

Query: 259  RFNNEFDSLFL-NQNEEEDIFKSQSAQYKDKVFDILNNGKARLPLSDEELYNIQMEIEDL 318
            RF NEF +  L N N+     KS S QYK+  FDIL   K RLPLS +EL  IQ +IE++
Sbjct: 26   RFWNEFYTYLLQNTNKSGQHGKSHSVQYKETAFDILEEAKTRLPLSVDELSEIQKQIEEI 85

Query: 319  TMTFDEESM--------------NEKC--------KLSEYDTGLLSEFGQYTIEALIVYV 378
            T+ FDE+S+              NE+            + D  LLSEFGQYTIEALIV+V
Sbjct: 86   TIWFDEKSIFRSASDIIKILIRENEESAKDFLRRRPFEKDDLELLSEFGQYTIEALIVHV 145

Query: 379  LCMVFTLPYNENLMIRLASLVDQLDTNVRSQALLLKSRRCKQSFSTVREE--VKKSGSGD 438
            L M F      N +IR+ASLV+QL+++VR QA LLKS RC + FS+   +  VKKSG   
Sbjct: 146  LSMFF-YSVESNSLIRVASLVEQLESSVRHQASLLKSGRCNKPFSSATNDFKVKKSGKDR 205

Query: 439  K-------YTIGSKLVSFMVERELIKLISCDLSGSVRAIKKKKESYFLPSHLYAVCNFDI 498
            K       Y  GS LV FM ER+LI L++ DLSG+VR +KKKK SYFLPSHLYAVCNFDI
Sbjct: 206  KRSKLVMMYPFGSGLVQFMEERKLISLVT-DLSGTVR-VKKKKGSYFLPSHLYAVCNFDI 265

Query: 499  SLLPIKFNLPMVYKPVDWVCARGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLSSGDINH 558
            SLLPIK NLPMV KP DW  A      PR+LSDLSGGYLSG TG +Y+RYRLLSSGDINH
Sbjct: 266  SLLPIKLNLPMVCKPRDWTSACRGDQNPRYLSDLSGGYLSGPTGGLYDRYRLLSSGDINH 325

Query: 559  FYIDI--ENRYEDLCGVMNKLQHQAFQINT------------------------------ 618
            FYIDI  E  YE LC VMNKLQ QAFQIN+                              
Sbjct: 326  FYIDIGREKNYEKLCLVMNKLQGQAFQINSHWLKCLKYNEDSFVESGLLMPRFLSSMNIK 385

Query: 619  ----------------------------------------------LAYDGYQFDLPAFL 678
                                                           AY+GY F LPAFL
Sbjct: 386  DVSNLLREFHMKDEVINKLCNFSELLHTLSKNIQRSRYENLIMKLAQAYEGYHFYLPAFL 445

Query: 679  DFRGRIYRCGILHFHERDLARSLIIFADKKDEDIKLSVGNNFLPKSFFAATAFHDQSFDS 738
            DFRGRIYR G+LHFHERDLARS+I+FAD K          N    ++ AA AFH +SF S
Sbjct: 446  DFRGRIYRSGVLHFHERDLARSMIVFADIKS-------SGNIDMNAYLAAAAFHYKSFVS 505

Query: 739  VDDATSWFCNNMMLPQISDHQILSYTLDAKRPFQFLANMI--TSNR----DQIPITQDAS 798
            VD+A  +F NN  L    D  +L Y  +AKRPFQ  A++I  TS       +IP+TQDAS
Sbjct: 506  VDEAL-YFSNNNFLQLSHDDDLLMYAREAKRPFQLFAHLIGVTSPNLKVITRIPLTQDAS 565

Query: 799  ASAYQIMSDFLLDETLAKRTNLIPSSDKKIQDIYSLRLEELKEFIVAELEDKNRSMLVCE 858
            ASAYQIMS FLLDE+LA RTNLIPS D KIQD+YS  LE+LK F+ AEL++ + S +VC 
Sbjct: 566  ASAYQIMSYFLLDESLASRTNLIPSLDGKIQDVYSFILEDLKVFMKAELDNNHLSTIVCN 625

Query: 859  LFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIASVCFKFWRTKYHHMEC 918
            + TRKLVK IFMP+IYGKTLMS A DLKD LS +IT KE F +ASVCFKFWRT+Y + EC
Sbjct: 626  VLTRKLVKGIFMPMIYGKTLMSTASDLKDTLSRFITHKECFDVASVCFKFWRTQYQNTEC 685

Query: 919  LIRLIRNIGWIASACERPVYESVPDFKTVQETMKMEPNNIWVYDKLHKKKRRVTLRVASS 978
            LIRLIR+IGWIASA + PV+  VP F TVQ+ MKM+P N+W YD LHKK+RRVTLRV+SS
Sbjct: 686  LIRLIRHIGWIASARDSPVFYRVPSFTTVQDYMKMDPINVWFYDGLHKKRRRVTLRVSSS 745

Query: 979  KRDRRKTAISTFVNFIHQRDASIAMKVVEFMQHRGAPIYTVHDNFITTAEYSEFLPDYYS 1028
            KRDRRKT ISTFVNFIHQRDA IAMKVVE M  +GAPIYTVHDNFITTAEYS FLP  Y 
Sbjct: 746  KRDRRKTEISTFVNFIHQRDAHIAMKVVECMLEKGAPIYTVHDNFITTAEYSYFLPIIYI 805

BLAST of Sgr022489 vs. NCBI nr
Match: CAB4263363.1 (unnamed protein product [Prunus armeniaca] >CAB4320324.1 unnamed protein product [Prunus armeniaca])

HSP 1 Score: 837.0 bits (2161), Expect = 1.9e-238
Identity = 493/894 (55.15%), Postives = 579/894 (64.77%), Query Frame = 0

Query: 259  RFNNEFDSLFL-NQNEEEDIFKSQSAQYKDKVFDILNNGKARLPLSDEELYNIQMEIEDL 318
            RF NEF +  L N N+     KS S QYK+  FDIL   K RLPLS +EL  IQ +IE++
Sbjct: 26   RFWNEFYTYLLQNTNKSGQHGKSHSVQYKETAFDILEEAKTRLPLSVDELSEIQKQIEEI 85

Query: 319  TMTFDEESM--------------NEKC--------KLSEYDTGLLSEFGQYTIEALIVYV 378
            T+ FDE+S+              NE+            + D  LLSEFGQYTIEALIV+V
Sbjct: 86   TIWFDEKSIFRSASDIIKILIRENEESAKDFLRRRPFEKDDLELLSEFGQYTIEALIVHV 145

Query: 379  LCMVFTLPYNENLMIRLASLVDQLDTNVRSQALLLKSRRCKQSFSTVREE--VKKSGSGD 438
            L M F      N +IR+ASLV+QL+++VR QA LLKS RC + FS+   +  VKKSG   
Sbjct: 146  LSMFF-YSVESNSLIRVASLVEQLESSVRHQASLLKSGRCNKPFSSATNDFKVKKSGKDR 205

Query: 439  K-------YTIGSKLVSFMVERELIKLISCDLSGSVRAIKKKKESYFLPSHLYAVCNFDI 498
            K       Y  GS LV FM ER+LI L++ DLSG+VR +KKKK SYFLPSHLYAVCNFDI
Sbjct: 206  KRSKLVMMYPFGSGLVQFMEERKLISLVT-DLSGTVR-VKKKKGSYFLPSHLYAVCNFDI 265

Query: 499  SLLPIKFNLPMVYKPVDWVCARGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLSSGDINH 558
            SLLPIK NLPMV KP DW  A      PR+LSDLSGGYLSG TG +Y+RYRLLSSGDINH
Sbjct: 266  SLLPIKLNLPMVCKPRDWTSACRGDQNPRYLSDLSGGYLSGPTGGLYDRYRLLSSGDINH 325

Query: 559  FYIDI--ENRYEDLCGVMNKLQHQAFQINT------------------------------ 618
            FYIDI  E  YE LC VMNKLQ QAFQIN+                              
Sbjct: 326  FYIDIGREKNYEKLCLVMNKLQGQAFQINSHWLKCLKYNEDSFVESGLLMPRFLSSMNIK 385

Query: 619  ----------------------------------------------LAYDGYQFDLPAFL 678
                                                           AY+GY F LPAFL
Sbjct: 386  DVSNLLREFHMKDEVINKLCNFSELLHTLSKNIQRSRYENLIMKLAQAYEGYHFYLPAFL 445

Query: 679  DFRGRIYRCGILHFHERDLARSLIIFADKKDEDIKLSVGNNFLPKSFFAATAFHDQSFDS 738
            DFRGRIYR G+LHFHERDLARS+I+FAD K          N    ++ AA AFH +SF S
Sbjct: 446  DFRGRIYRSGVLHFHERDLARSMIVFADIKS-------SGNIDMNAYLAAAAFHYKSFVS 505

Query: 739  VDDATSWFCNNMMLPQISDHQILSYTLDAKRPFQFLANMI--TSNR----DQIPITQDAS 798
            VD+A  +F NN  L    D  +L Y  +AKRPFQ  A++I  TS       +IP+TQDAS
Sbjct: 506  VDEAL-YFSNNNFLQLSHDDDLLMYAREAKRPFQLFAHLIGVTSPNLKVITRIPLTQDAS 565

Query: 799  ASAYQIMSDFLLDETLAKRTNLIPSSDKKIQDIYSLRLEELKEFIVAELEDKNRSMLVCE 858
            ASAYQIMS FLLDE+LA RTNLIPS D KIQD+YS  LE+LK F+ AEL++ + S +VC 
Sbjct: 566  ASAYQIMSYFLLDESLASRTNLIPSLDGKIQDVYSFILEDLKVFMKAELDNNHLSTIVCN 625

Query: 859  LFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIASVCFKFWRTKYHHMEC 918
            + TRKLVK IFMP+IYGKTLMS A DLKD LS +IT KE F +ASVCFKFWRT+Y + EC
Sbjct: 626  VLTRKLVKGIFMPMIYGKTLMSTASDLKDTLSRFITHKECFDVASVCFKFWRTQYQNTEC 685

Query: 919  LIRLIRNIGWIASACERPVYESVPDFKTVQETMKMEPNNIWVYDKLHKKKRRVTLRVASS 978
            LIRLIR+IGWIASA + PV+  VP F TVQ+ MKM+P N+W YD LHKK+RRVTLRV+SS
Sbjct: 686  LIRLIRHIGWIASARDSPVFYRVPSFTTVQDYMKMDPINVWFYDGLHKKRRRVTLRVSSS 745

Query: 979  KRDRRKTAISTFVNFIHQRDASIAMKVVEFMQHRGAPIYTVHDNFITTAEYSEFLPDYYS 1028
            KRDRRKT ISTFVNFIHQRDA IAMKVVE M  +GAPIYTVHDNFITTAEYS FLP  Y 
Sbjct: 746  KRDRRKTEISTFVNFIHQRDAHIAMKVVECMLEKGAPIYTVHDNFITTAEYSYFLPIIYI 805

BLAST of Sgr022489 vs. NCBI nr
Match: CAB4293876.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 810.8 bits (2093), Expect = 1.5e-230
Identity = 482/894 (53.91%), Postives = 564/894 (63.09%), Query Frame = 0

Query: 259  RFNNEFDSLFL-NQNEEEDIFKSQSAQYKDKVFDILNNGKARLPLSDEELYNIQMEIEDL 318
            RF NEF +  L N N+     KS S QYK+  FDIL   K RLPLS +EL  IQ +IE++
Sbjct: 26   RFWNEFYTYLLQNTNKSGQHGKSHSVQYKETAFDILEEAKTRLPLSVDELSEIQKQIEEI 85

Query: 319  TMTFDEESM--------------NEKC--------KLSEYDTGLLSEFGQYTIEALIVYV 378
            T+ FDE+S+              NE+            + D  LLSEFGQYTIEALIV+V
Sbjct: 86   TIWFDEKSIFRSASDIIKILIRENEESAKDFLRRRPFEKDDLELLSEFGQYTIEALIVHV 145

Query: 379  LCMVFTLPYNENLMIRLASLVDQLDTNVRSQALLLKSRRCKQSFSTVREE--VKKSGSGD 438
                                   L ++VR QA LLKS RC + FS+   +  VKKSG   
Sbjct: 146  -----------------------LKSSVRHQASLLKSGRCNKPFSSATNDFKVKKSGKDR 205

Query: 439  K-------YTIGSKLVSFMVERELIKLISCDLSGSVRAIKKKKESYFLPSHLYAVCNFDI 498
            K       Y  GS LV FM ER+LI L++ DLSG+VR +KKKK SYFLPSHLYAVCNFDI
Sbjct: 206  KRSKLVMMYPFGSGLVQFMEERKLISLVT-DLSGTVR-VKKKKGSYFLPSHLYAVCNFDI 265

Query: 499  SLLPIKFNLPMVYKPVDWVCARGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLSSGDINH 558
            SLLPIK NLPMV KP DW  A      PR+LSDLSGGYLSG TG +Y+RYRLLSSGDINH
Sbjct: 266  SLLPIKLNLPMVCKPRDWTSACRGDQNPRYLSDLSGGYLSGPTGGLYDRYRLLSSGDINH 325

Query: 559  FYIDI--ENRYEDLCGVMNKLQHQAFQINT------------------------------ 618
            FYIDI  E  YE LC VMNKLQ QAFQIN+                              
Sbjct: 326  FYIDIGREKNYEKLCLVMNKLQGQAFQINSHWLKCLKYNEDSFVESGLLMPRFLSSMNIK 385

Query: 619  ----------------------------------------------LAYDGYQFDLPAFL 678
                                                           AY+GY F LPAFL
Sbjct: 386  DVSNLLREFHMKDEVINKLCNFSELLHTLSKNIQRSRYENLIMKLAQAYEGYHFYLPAFL 445

Query: 679  DFRGRIYRCGILHFHERDLARSLIIFADKKDEDIKLSVGNNFLPKSFFAATAFHDQSFDS 738
            DFRGRIYR G+LHFHERDLARS+I+FAD K          N    ++ AA AFH +SF S
Sbjct: 446  DFRGRIYRSGVLHFHERDLARSMIVFADIKS-------SGNIDMNAYLAAAAFHYKSFVS 505

Query: 739  VDDATSWFCNNMMLPQISDHQILSYTLDAKRPFQFLANMI--TSNR----DQIPITQDAS 798
            VD+A  +F NN  L    D  +L Y  +AKRPFQ  A++I  TS       +IP+TQDAS
Sbjct: 506  VDEAL-YFSNNNFLQLSHDDDLLMYAREAKRPFQLFAHLIGVTSPNLKVITRIPLTQDAS 565

Query: 799  ASAYQIMSDFLLDETLAKRTNLIPSSDKKIQDIYSLRLEELKEFIVAELEDKNRSMLVCE 858
            ASAYQIMS FLLDE+LA RTNLIPS D KIQD+YS  LE+LK F+ AEL++ + S +VC 
Sbjct: 566  ASAYQIMSYFLLDESLASRTNLIPSLDGKIQDVYSFILEDLKVFMKAELDNNHLSTIVCN 625

Query: 859  LFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIASVCFKFWRTKYHHMEC 918
            + TRKLVK IFMP+IYGKTLMS A DLKD LS +IT KE F +ASVCFKFWRT+Y + EC
Sbjct: 626  VLTRKLVKGIFMPMIYGKTLMSTASDLKDTLSRFITHKECFDVASVCFKFWRTQYQNTEC 685

Query: 919  LIRLIRNIGWIASACERPVYESVPDFKTVQETMKMEPNNIWVYDKLHKKKRRVTLRVASS 978
            LIRLIR+IGWIASA + PV+  VP F TVQ+ MKM+P N+W YD LHKK+RRVTLRV+SS
Sbjct: 686  LIRLIRHIGWIASARDSPVFYRVPSFTTVQDYMKMDPINVWFYDGLHKKRRRVTLRVSSS 745

Query: 979  KRDRRKTAISTFVNFIHQRDASIAMKVVEFMQHRGAPIYTVHDNFITTAEYSEFLPDYYS 1028
            KRDRRKT ISTFVNFIHQRDA IAMKVVE M  +GAPIYTVHDNFITTAEYS FLP  Y 
Sbjct: 746  KRDRRKTEISTFVNFIHQRDAHIAMKVVECMLEKGAPIYTVHDNFITTAEYSYFLPIIYI 805

BLAST of Sgr022489 vs. NCBI nr
Match: XP_017985382.1 (PREDICTED: probable DNA-directed RNA polymerase [Theobroma cacao])

HSP 1 Score: 801.2 bits (2068), Expect = 1.2e-227
Identity = 469/868 (54.03%), Postives = 570/868 (65.67%), Query Frame = 0

Query: 280  SQSAQYKDKVFDILNNGKARLPLSDEELYNIQMEIEDLTMTFDEESMNE------KCKLS 339
            ++S QYK++VF +L+  K   PLS++ L ++QMEIED+TM FDE+SM +      K  + 
Sbjct: 8    TKSEQYKNEVFILLDKAKELFPLSEDLLRDLQMEIEDMTMLFDEQSMFKSVPNIIKILIR 67

Query: 340  EYDTG----------------LLSEFGQYTIEALIVYVLCMVFTLPYNENLMIRLASLVD 399
            +YD                  LLSEFGQYTIEALIV+VL M+F  P     +IR+ASLV+
Sbjct: 68   DYDVSAKDYLRGMELEKDDIELLSEFGQYTIEALIVHVLGMLFN-PLENYSIIRVASLVE 127

Query: 400  QLDTNVRSQALLLKSRRCKQSFSTVREEVKK---SGSGDK-------YTIGSKLVSFMVE 459
            +L+ +VR QALLLKSRRCK+ FS V++EV+K   SG   K       Y  GS LV FM E
Sbjct: 128  RLEYSVRIQALLLKSRRCKKPFSMVKDEVEKVKFSGKERKRSKLVIMYPFGSGLVQFMEE 187

Query: 460  RELIKLISCDLSGSVRAIKKKKESYFLPSHLYAVCNFDISLLPIKFNLPMVYKPVDWVCA 519
            R LI L S DL G+VR ++KKK +YFLPS+L+A+CNFDISLLPIK NLPMV  P+DW  A
Sbjct: 188  RGLITLTS-DLIGTVR-VQKKKGAYFLPSNLFALCNFDISLLPIKLNLPMVCPPLDWKSA 247

Query: 520  RGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLSSGDINHFYIDI--ENRYEDLCGVMNKL 579
               G +PR LSDLSGGYLSG TGEIY+RYRLLS+GDI +FYIDI  +N Y  LC VMNKL
Sbjct: 248  SQHGQEPRTLSDLSGGYLSGPTGEIYDRYRLLSTGDIYNFYIDIGRDNNYMYLCSVMNKL 307

Query: 580  QHQAFQINT--------------------------------------------------- 639
            Q Q FQIN+                                                   
Sbjct: 308  QRQPFQINSDWLKYIQINEDLFVEYGYLMPKFLSSMNIKNVSFLLREFYMKDEVINKICS 367

Query: 640  -------------------------LAYDGYQFDLPAFLDFRGRIYRCGILHFHERDLAR 699
                                     +AY+GY+F LPAFLDFRGRIYR G+LHFHERDLAR
Sbjct: 368  FNELLQTLNKSIQRARYEQLIIKLAIAYEGYKFYLPAFLDFRGRIYRSGVLHFHERDLAR 427

Query: 700  SLIIFADKKDEDIKLSVGNNFLPKSFFAATAFHDQSFDSVDDATSWFCNNMMLPQISDHQ 759
            SLI+FAD    + +          +  AA AFH + F SV++A  W+  N +  QI  + 
Sbjct: 428  SLIVFADCNSMESRKK-------DNVIAAAAFHYKPFKSVNNALLWYYENKL--QIESNS 487

Query: 760  ILSYTLDAKRPFQFLANMI---TSNRDQIPITQDASASAYQIMSDFLLDETLAKRTNLIP 819
            +L +TL+AKR FQF+AN I   T   + +PI QDASASAYQIMS FLLDE+LAKRTNLIP
Sbjct: 488  LL-FTLEAKRNFQFIANQIGIFTGKWNCLPIIQDASASAYQIMSYFLLDESLAKRTNLIP 547

Query: 820  SSDKKIQDIYSLRLEELKEFIVAELEDKNRSMLVCELFTRKLVKSIFMPIIYGKTLMSIA 879
            S D ++QD+YS  LEELKEF+ AEL + N S +VC++  RK+VK IFMPIIYGKTLMS A
Sbjct: 548  SPDGEMQDVYSFLLEELKEFMKAELAN-NLSTIVCDIIDRKIVKGIFMPIIYGKTLMSTA 607

Query: 880  GDLKDHLSIYITFKESFIIASVCFKFWRTKYHHMECLIRLIRNIGWIASACERPVYESVP 939
             DLK HLS +IT KE + IASVCFKFWRTKY  + CLIRLIR+IGWI SA   PV+  VP
Sbjct: 608  NDLKVHLSHFITHKECYEIASVCFKFWRTKYQGIPCLIRLIRHIGWIVSARNSPVFYRVP 667

Query: 940  DFKTVQETMKMEPNNIWVYDKLHKKKRRVTLRVASSKRDRRKTAISTFVNFIHQRDASIA 999
             F TVQ+ M MEP NIWVYD+LHKK+RRVTLRV+SSKRDRRKT ISTFVNFIHQ+DA IA
Sbjct: 668  FFTTVQDYMIMEPINIWVYDRLHKKRRRVTLRVSSSKRDRRKTEISTFVNFIHQKDAHIA 727

Query: 1000 MKVVEFMQHRGAPIYTVHDNFITTAEYSEFLPDYYSSTICEMGPPLQILNEFIHMNVIQP 1029
            M VVE M   GAPIYTVHDNFI+TA+YS+ +P  YS+ IC M PPL ++NEFI+MNVI+P
Sbjct: 728  MCVVEQMLSIGAPIYTVHDNFISTAQYSDLIPSIYSNVICNMDPPLSVINEFIYMNVIKP 787

BLAST of Sgr022489 vs. NCBI nr
Match: KAF3452229.1 (hypothetical protein FNV43_RR08327 [Rhamnella rubrinervis])

HSP 1 Score: 776.9 bits (2005), Expect = 2.4e-220
Identity = 461/916 (50.33%), Postives = 580/916 (63.32%), Query Frame = 0

Query: 259  RFNNEFDSLFLNQNEEEDIFKSQSAQYKDKVFDILNNGKARLPLSDEELYNIQMEIEDLT 318
            RF N F + F  +       +++S QYK KVFD L   K   PL  EEL  +Q+E+ED  
Sbjct: 30   RFWNNFYTEFDKKQIHSVNNETKSEQYKSKVFDKLEKAKNGQPLGPEELRKLQLEMEDEI 89

Query: 319  MTFDEESM-----------------NEKCKL-----SEYDTGLLSEFGQYTIEALIVYVL 378
              FDEES+                 N K  L      + D  LLSEFGQYTIEALIV+VL
Sbjct: 90   FQFDEESIYKTLPNIIKNLIKINDQNAKDYLRSQIKQKDDNELLSEFGQYTIEALIVHVL 149

Query: 379  CMVF-TLPYNENLMIRLASLVDQLDTNVRSQALLLKSRRCKQSF--STVREEVKK---SG 438
             +VF +L YN   ++R+A+L+++L+++VR+QA LLK RRCK+    ST  ++V K   SG
Sbjct: 150  SLVFNSLEYNS--LVRVATLIERLESSVRTQAALLKHRRCKKDLTSSTGTDDVNKVNLSG 209

Query: 439  S----------GDKYTIGSKLVSFMVERELIKLISCDLSGSVRAIKKKKESYFLPSHLYA 498
                         KY  GS L+ FM++R LI +I+    G    ++ K  SY LP ++ A
Sbjct: 210  KDREKKMITEYDPKYPFGSNLIEFMMQRGLITIIND--QGDKITVRNKVGSYHLPKNVCA 269

Query: 499  VCNFDISLLPIKFNLPMVYKPVDWVCARGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLS 558
             CNFDISLLPIK NLPMVY P+DW  A   G  PR+LSDLSGGYLSG  GEIY+RYRLLS
Sbjct: 270  FCNFDISLLPIKLNLPMVYPPLDWTSACPDGQNPRYLSDLSGGYLSGPAGEIYDRYRLLS 329

Query: 559  SGDINHFYIDI--ENRYEDLCGVMNKLQHQAFQINT------------------------ 618
            SGDINHFYIDI  EN Y+ LC VMNKLQ Q F IN+                        
Sbjct: 330  SGDINHFYIDIGQENNYDRLCQVMNKLQSQPFSINSDWLNYIQANEHLLVEYGYLMPRFL 389

Query: 619  ----------------------------------------------------LAYDGYQF 678
                                                                 AYDGYQF
Sbjct: 390  ASMNIKDVSNSLRESHMKDDVIRQLCSFSELLHTLCKNIQRSRYEQLIIKLAKAYDGYQF 449

Query: 679  DLPAFLDFRGRIYRCGILHFHERDLARSLIIFADKKDEDIKLSVGNNF-------LPKSF 738
             LPAFLDFRGRIYRCGILHFHERDLARS+I+FADK   D  + + +N+          + 
Sbjct: 450  YLPAFLDFRGRIYRCGILHFHERDLARSMIVFADKTSND-NVDMTSNYDDDQIISYNLTA 509

Query: 739  FAATAFHDQSFDSVDDATSWFCNNMMLPQISDHQILSYTLDAKRPFQFLANMITSNRDQI 798
            +AA AFH +SF+S++DA +WF +   +  I ++ I  +  +AKRPFQFL+N+I  +++  
Sbjct: 510  YAAAAFHYKSFNSINDALNWFLSEKWV--IWENPI-GFAREAKRPFQFLSNLIAISKNSF 569

Query: 799  ------PITQDASASAYQIMSDFLLDETLAKRTNLIPSSDKKIQDIYSLRLEELKEFIVA 858
                  PITQDASASAYQIMS FLLDE +AKRTNL PS + +IQD+Y++ L+ELKE+I  
Sbjct: 570  LSHKITPITQDASASAYQIMSYFLLDEIMAKRTNLFPSPEDQIQDVYTMILKELKEYIQE 629

Query: 859  E--LEDKNRSMLVCELFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIAS 918
            E  +E KN S LVC L TRK+VK IFMPIIYGKT+MS A DLKDHLS YIT+KE F +AS
Sbjct: 630  EWCVEKKNLSSLVCNLLTRKIVKGIFMPIIYGKTVMSTASDLKDHLSHYITYKECFEVAS 689

Query: 919  VCFKFWRTKYHHMECLIRLIRNIGWIASACERPVYESVPDFKTVQETMKMEPNNIWVYDK 978
            +CFKFWRTKY  M+CLIRLIR+IGWIASA   PV   VP F+TVQ+ MKME   IW+Y+K
Sbjct: 690  LCFKFWRTKYLGMDCLIRLIRHIGWIASARGSPVIYKVPYFRTVQDYMKMEGIYIWIYNK 749

Query: 979  LHKKKRRVTLRVASSKRDRRKTAISTFVNFIHQRDASIAMKVVEFMQHRGAPIYTVHDNF 1031
            LHKK+ RVTLRV+SSKRDRRKT ISTFVNFIHQRDA IAM  VE+M    APIYTVHDNF
Sbjct: 750  LHKKRHRVTLRVSSSKRDRRKTEISTFVNFIHQRDAYIAMHAVEYMLLHDAPIYTVHDNF 809

BLAST of Sgr022489 vs. ExPASy Swiss-Prot
Match: P10581 (Probable DNA-directed RNA polymerase OS=Zea mays OX=4577 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 6.6e-64
Identity = 245/882 (27.78%), Postives = 376/882 (42.63%), Query Frame = 0

Query: 263 EFDSLFLNQNEEEDIFKS--QSAQYKDKVFD-ILNNGKARLPLSDEELYNIQMEIEDLTM 322
           +F   F+   E E +  S  +    KDKVF+ +L++   +   + EEL  IQ +IE +TM
Sbjct: 84  QFWDEFIETYENEVMMNSGYREPLNKDKVFNTLLDHSNNKTNQTAEELKGIQTKIERMTM 143

Query: 323 TFDEESMNEKC---------------------KLSE-YDTGLLSEFGQYTIEALIVYVLC 382
            F E ++ E                       KL +  D  ++   G YT+E ++V+VL 
Sbjct: 144 YFYEANVYESLGQLKNKFINIDEQTAKDYLLDKLEKPDDLDIVRAMGTYTLECIVVFVLS 203

Query: 383 MVFTLPYNENLMIRLASLVDQLDTNVR-------------------------SQALLLKS 442
             F +   +   +++A+LV +LD+  +                          + +LL+ 
Sbjct: 204 KQFNVFALDKASVQVATLVSELDSAAKIEYNRIIAEDRKRKKAKWGDKQINEDENILLQD 263

Query: 443 RRCKQSFSTVREEV-KKSGSGDKYT--------------IGSKLVSFMVERELIKLISCD 502
           +        V  E+ +K  S  K T              IG  L  ++  R+LI++    
Sbjct: 264 KGVDTFNGDVHSELDRKKKSISKQTNRKRVRISKHKEPGIGEALFGWLASRKLIEVKKPV 323

Query: 503 LSGSVRAIKKKKESYFLPSHLYAVCNFDISLLPIKFNLPMVYKPVD---WVCARGIGHKP 562
                +   KK ++   P   Y  C FDI  LP    LPMVY P D   W  A      P
Sbjct: 324 FLFDKK--NKKPKNVIYPG--YVDCLFDIKDLPFCSTLPMVYPPADWELWPTATAEVSDP 383

Query: 563 -----RHLSDLSGGYLSGLTGEIYNRYRLLSSGDINHFYIDIE-NRYEDLCGVMNKLQHQ 622
                  LS   GGYL+ L  E  +   LLS  D   F I I+  R + +   + KLQ Q
Sbjct: 384 YITNLTPLSSYRGGYLTSLQRESGDSPTLLSEKDYGVFDIHIDRERSQPVLSAVKKLQWQ 443

Query: 623 AFQINTLAYD-------------------------------------------------- 682
            ++IN L YD                                                  
Sbjct: 444 PYRINKLVYDFIQKHWSVLVSVGLLRPKNLALFKRKEALRLLSSLLFKHEELSTIYRYSE 503

Query: 683 --------------------------GYQFDLPAFLDFRGRIYRCGILHFHERDLARSLI 742
                                      Y+   P FLDFRGR YR G  HFHERDL RSLI
Sbjct: 504 LKSVLLKNIHASTFELYTMKIAEAYLDYKIYFPIFLDFRGRNYRHGPFHFHERDLVRSLI 563

Query: 743 IFADKKD---EDIKLSVGNNFLPKSFFAATAFHDQSFDSVDDATSWFCNNM----MLPQI 802
           IF +  D     I   VG+  L  +F  + A+H   F    +A  +  N +      P  
Sbjct: 564 IFDESDDSAAHTINSDVGDRIL-HNFLISAAYHKSKFGVYREALEFIYNKIEDMQSKPTF 623

Query: 803 SDHQILSYTLDAKRPFQFLANMIT----------SNRDQIPITQDASASAYQIMSDFLLD 862
            +  I   TL  + PFQ++++ I+          S     P+ QDASASAYQIMS FLLD
Sbjct: 624 FEKDIFVDTLCCRHPFQYISSCISLKTYADTKDLSVLRYTPVFQDASASAYQIMSYFLLD 683

Query: 863 ETLAKRTNLIPSSD---KKIQDIYSLRLEELKEFIVAE------------LEDKNRSML- 922
                 TNL+  ++   + I+DIY      L ++++AE             E    S+L 
Sbjct: 684 IDYGIHTNLLKKTNTDGRYIRDIYEFMWGCLIKYLIAEEKIELAIKLLTPNEKDQESVLA 743

Query: 923 -VCELFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIASVCFKFWRTKYH 949
            +  +F R +VK +FMP++YGKT  ++  D++D L      +   +I+     +W+  + 
Sbjct: 744 KIVSIFDRNVVKKMFMPMMYGKTDYTLKKDVEDLLKGKSDSEGINLISKHISTYWKVNFG 803

BLAST of Sgr022489 vs. ExPASy Swiss-Prot
Match: P33539 (Probable DNA-directed RNA polymerase OS=Agaricus bitorquis OX=5343 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 5.1e-16
Identity = 100/399 (25.06%), Postives = 170/399 (42.61%), Query Frame = 0

Query: 557 YDGYQFDLPAFLDFRGRIY-RCGILHFHERDLARSLIIFADKKDEDIKLSVGNNFLPKSF 616
           Y   +F LP ++DFRGR+Y     + +   DLARSLI+FAD K     L+         +
Sbjct: 602 YKDNEFYLPVYVDFRGRVYPLSNYISYQGGDLARSLILFADTK---CVLNNSGKECLNVY 661

Query: 617 FAATAFHD-----QSFDSVDDATSWFCNNMMLPQISDHQILSYTLD-AKRPFQFLANM-- 676
            A  A +D     +    VD     +  +    +IS+ + +   +D    PFQF++ M  
Sbjct: 662 LANLAGYDKLPWSERLTKVDGIIKEYLES---NEISNTKYIEDNIDKISEPFQFISIMYA 721

Query: 677 ----ITSNRDQI--PITQDASASAYQIMSDFLLDETLAKRTNLIPSS----DKKIQDIYS 736
               I++ +  I  PI  DAS S  Q ++   L++ LA   NL   S    +   QD Y+
Sbjct: 722 KLLSISNPKANISNPILFDASCSGIQHIAALTLEKELASNVNLYTDSSNPKEDYPQDFYT 781

Query: 737 LRLEELKEFI----VAELEDKNRSMLVCELFTRKLVKSIFMPIIYGKTLMSIAGDLKDHL 796
             LE++++ +    + EL D            RK++K   M I Y  ++  I   L +H 
Sbjct: 782 YALEKIRDKLINSDITELRDIK--------LNRKIIKRSVMTIPYNISMAGIGEHLMEHF 841

Query: 797 SIYITFKESFII----ASVCFKFWRTKYHHMECLIRLIRNI------------GWIASAC 856
           ++    K  +++    A++  K     Y     L ++I  +             +  S  
Sbjct: 842 TVKTVLKYRYVVIPGSATISSKDVYLDYSKYGQLCKIIYFVLTKELPSLRLLSNYFESMI 901

Query: 857 ERPVYESVPDFKTVQETMKMEPNNI-----WVYDKLHKKKRRVTLRVASSKRDRRKTAIS 912
           +  V  ++P        +K++  NI      V   +    +  T+++ +   D   T  S
Sbjct: 902 DIFVKLNIPITWVTPSGLKIKYTNIKFKPQKVKTSVLNTSKITTIKLPTDSLDVLSTKRS 961

BLAST of Sgr022489 vs. ExPASy Swiss-Prot
Match: P33544 (Uncharacterized 33.9 kDa protein in mitochondrial linear 2.3 KB plasmid OS=Zea mays OX=4577 PE=4 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 5.9e-04
Identity = 47/158 (29.75%), Postives = 75/158 (47.47%), Query Frame = 0

Query: 268 FLNQNEEEDIFKSQSAQ--YKDKVFDIL-NNGKARLPLSDEELYNIQMEIEDLTMTFDEE 327
           FL   E+E I + Q+     K KVFD+L +        + EEL  IQ +IE LT+ FDE 
Sbjct: 137 FLGSYEKEGINRKQNTDPVSKGKVFDLLASRSSNNTNQTPEELREIQTQIEHLTIHFDEG 196

Query: 328 SMNEKC---------------------KL-SEYDTGLLSEFGQYTIEALIVYVLCMVFTL 387
           S++E                       KL  + D  ++   G YT+E + ++VL  +F +
Sbjct: 197 SVHESAGQLKGKFFNKDRQSARDYLLDKLKDDKDKDIVRGMGTYTLECVAIHVLSKLFNV 256

Query: 388 PYNENLMIRLASLVDQLDTNVRSQ---ALLLKSRRCKQ 398
              E   +  A+L+ +LD   +++   ALL K +R K+
Sbjct: 257 FSLEKTSVLAAALISELDITAKTEYNNALLAKMQREKE 294

BLAST of Sgr022489 vs. ExPASy TrEMBL
Match: A0A6J5Y9W3 (DNA-directed RNA polymerase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS3511 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 9.4e-239
Identity = 493/894 (55.15%), Postives = 579/894 (64.77%), Query Frame = 0

Query: 259  RFNNEFDSLFL-NQNEEEDIFKSQSAQYKDKVFDILNNGKARLPLSDEELYNIQMEIEDL 318
            RF NEF +  L N N+     KS S QYK+  FDIL   K RLPLS +EL  IQ +IE++
Sbjct: 26   RFWNEFYTYLLQNTNKSGQHGKSHSVQYKETAFDILEEAKTRLPLSVDELSEIQKQIEEI 85

Query: 319  TMTFDEESM--------------NEKC--------KLSEYDTGLLSEFGQYTIEALIVYV 378
            T+ FDE+S+              NE+            + D  LLSEFGQYTIEALIV+V
Sbjct: 86   TIWFDEKSIFRSASDIIKILIRENEESAKDFLRRRPFEKDDLELLSEFGQYTIEALIVHV 145

Query: 379  LCMVFTLPYNENLMIRLASLVDQLDTNVRSQALLLKSRRCKQSFSTVREE--VKKSGSGD 438
            L M F      N +IR+ASLV+QL+++VR QA LLKS RC + FS+   +  VKKSG   
Sbjct: 146  LSMFF-YSVESNSLIRVASLVEQLESSVRHQASLLKSGRCNKPFSSATNDFKVKKSGKDR 205

Query: 439  K-------YTIGSKLVSFMVERELIKLISCDLSGSVRAIKKKKESYFLPSHLYAVCNFDI 498
            K       Y  GS LV FM ER+LI L++ DLSG+VR +KKKK SYFLPSHLYAVCNFDI
Sbjct: 206  KRSKLVMMYPFGSGLVQFMEERKLISLVT-DLSGTVR-VKKKKGSYFLPSHLYAVCNFDI 265

Query: 499  SLLPIKFNLPMVYKPVDWVCARGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLSSGDINH 558
            SLLPIK NLPMV KP DW  A      PR+LSDLSGGYLSG TG +Y+RYRLLSSGDINH
Sbjct: 266  SLLPIKLNLPMVCKPRDWTSACRGDQNPRYLSDLSGGYLSGPTGGLYDRYRLLSSGDINH 325

Query: 559  FYIDI--ENRYEDLCGVMNKLQHQAFQINT------------------------------ 618
            FYIDI  E  YE LC VMNKLQ QAFQIN+                              
Sbjct: 326  FYIDIGREKNYEKLCLVMNKLQGQAFQINSHWLKCLKYNEDSFVESGLLMPRFLSSMNIK 385

Query: 619  ----------------------------------------------LAYDGYQFDLPAFL 678
                                                           AY+GY F LPAFL
Sbjct: 386  DVSNLLREFHMKDEVINKLCNFSELLHTLSKNIQRSRYENLIMKLAQAYEGYHFYLPAFL 445

Query: 679  DFRGRIYRCGILHFHERDLARSLIIFADKKDEDIKLSVGNNFLPKSFFAATAFHDQSFDS 738
            DFRGRIYR G+LHFHERDLARS+I+FAD K          N    ++ AA AFH +SF S
Sbjct: 446  DFRGRIYRSGVLHFHERDLARSMIVFADIKS-------SGNIDMNAYLAAAAFHYKSFVS 505

Query: 739  VDDATSWFCNNMMLPQISDHQILSYTLDAKRPFQFLANMI--TSNR----DQIPITQDAS 798
            VD+A  +F NN  L    D  +L Y  +AKRPFQ  A++I  TS       +IP+TQDAS
Sbjct: 506  VDEAL-YFSNNNFLQLSHDDDLLMYAREAKRPFQLFAHLIGVTSPNLKVITRIPLTQDAS 565

Query: 799  ASAYQIMSDFLLDETLAKRTNLIPSSDKKIQDIYSLRLEELKEFIVAELEDKNRSMLVCE 858
            ASAYQIMS FLLDE+LA RTNLIPS D KIQD+YS  LE+LK F+ AEL++ + S +VC 
Sbjct: 566  ASAYQIMSYFLLDESLASRTNLIPSLDGKIQDVYSFILEDLKVFMKAELDNNHLSTIVCN 625

Query: 859  LFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIASVCFKFWRTKYHHMEC 918
            + TRKLVK IFMP+IYGKTLMS A DLKD LS +IT KE F +ASVCFKFWRT+Y + EC
Sbjct: 626  VLTRKLVKGIFMPMIYGKTLMSTASDLKDTLSRFITHKECFDVASVCFKFWRTQYQNTEC 685

Query: 919  LIRLIRNIGWIASACERPVYESVPDFKTVQETMKMEPNNIWVYDKLHKKKRRVTLRVASS 978
            LIRLIR+IGWIASA + PV+  VP F TVQ+ MKM+P N+W YD LHKK+RRVTLRV+SS
Sbjct: 686  LIRLIRHIGWIASARDSPVFYRVPSFTTVQDYMKMDPINVWFYDGLHKKRRRVTLRVSSS 745

Query: 979  KRDRRKTAISTFVNFIHQRDASIAMKVVEFMQHRGAPIYTVHDNFITTAEYSEFLPDYYS 1028
            KRDRRKT ISTFVNFIHQRDA IAMKVVE M  +GAPIYTVHDNFITTAEYS FLP  Y 
Sbjct: 746  KRDRRKTEISTFVNFIHQRDAHIAMKVVECMLEKGAPIYTVHDNFITTAEYSYFLPIIYI 805

BLAST of Sgr022489 vs. ExPASy TrEMBL
Match: A0A6J5VPQ6 (Multifunctional fusion protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS49732 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 9.4e-239
Identity = 493/894 (55.15%), Postives = 579/894 (64.77%), Query Frame = 0

Query: 259  RFNNEFDSLFL-NQNEEEDIFKSQSAQYKDKVFDILNNGKARLPLSDEELYNIQMEIEDL 318
            RF NEF +  L N N+     KS S QYK+  FDIL   K RLPLS +EL  IQ +IE++
Sbjct: 26   RFWNEFYTYLLQNTNKSGQHGKSHSVQYKETAFDILEEAKTRLPLSVDELSEIQKQIEEI 85

Query: 319  TMTFDEESM--------------NEKC--------KLSEYDTGLLSEFGQYTIEALIVYV 378
            T+ FDE+S+              NE+            + D  LLSEFGQYTIEALIV+V
Sbjct: 86   TIWFDEKSIFRSASDIIKILIRENEESAKDFLRRRPFEKDDLELLSEFGQYTIEALIVHV 145

Query: 379  LCMVFTLPYNENLMIRLASLVDQLDTNVRSQALLLKSRRCKQSFSTVREE--VKKSGSGD 438
            L M F      N +IR+ASLV+QL+++VR QA LLKS RC + FS+   +  VKKSG   
Sbjct: 146  LSMFF-YSVESNSLIRVASLVEQLESSVRHQASLLKSGRCNKPFSSATNDFKVKKSGKDR 205

Query: 439  K-------YTIGSKLVSFMVERELIKLISCDLSGSVRAIKKKKESYFLPSHLYAVCNFDI 498
            K       Y  GS LV FM ER+LI L++ DLSG+VR +KKKK SYFLPSHLYAVCNFDI
Sbjct: 206  KRSKLVMMYPFGSGLVQFMEERKLISLVT-DLSGTVR-VKKKKGSYFLPSHLYAVCNFDI 265

Query: 499  SLLPIKFNLPMVYKPVDWVCARGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLSSGDINH 558
            SLLPIK NLPMV KP DW  A      PR+LSDLSGGYLSG TG +Y+RYRLLSSGDINH
Sbjct: 266  SLLPIKLNLPMVCKPRDWTSACRGDQNPRYLSDLSGGYLSGPTGGLYDRYRLLSSGDINH 325

Query: 559  FYIDI--ENRYEDLCGVMNKLQHQAFQINT------------------------------ 618
            FYIDI  E  YE LC VMNKLQ QAFQIN+                              
Sbjct: 326  FYIDIGREKNYEKLCLVMNKLQGQAFQINSHWLKCLKYNEDSFVESGLLMPRFLSSMNIK 385

Query: 619  ----------------------------------------------LAYDGYQFDLPAFL 678
                                                           AY+GY F LPAFL
Sbjct: 386  DVSNLLREFHMKDEVINKLCNFSELLHTLSKNIQRSRYENLIMKLAQAYEGYHFYLPAFL 445

Query: 679  DFRGRIYRCGILHFHERDLARSLIIFADKKDEDIKLSVGNNFLPKSFFAATAFHDQSFDS 738
            DFRGRIYR G+LHFHERDLARS+I+FAD K          N    ++ AA AFH +SF S
Sbjct: 446  DFRGRIYRSGVLHFHERDLARSMIVFADIKS-------SGNIDMNAYLAAAAFHYKSFVS 505

Query: 739  VDDATSWFCNNMMLPQISDHQILSYTLDAKRPFQFLANMI--TSNR----DQIPITQDAS 798
            VD+A  +F NN  L    D  +L Y  +AKRPFQ  A++I  TS       +IP+TQDAS
Sbjct: 506  VDEAL-YFSNNNFLQLSHDDDLLMYAREAKRPFQLFAHLIGVTSPNLKVITRIPLTQDAS 565

Query: 799  ASAYQIMSDFLLDETLAKRTNLIPSSDKKIQDIYSLRLEELKEFIVAELEDKNRSMLVCE 858
            ASAYQIMS FLLDE+LA RTNLIPS D KIQD+YS  LE+LK F+ AEL++ + S +VC 
Sbjct: 566  ASAYQIMSYFLLDESLASRTNLIPSLDGKIQDVYSFILEDLKVFMKAELDNNHLSTIVCN 625

Query: 859  LFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIASVCFKFWRTKYHHMEC 918
            + TRKLVK IFMP+IYGKTLMS A DLKD LS +IT KE F +ASVCFKFWRT+Y + EC
Sbjct: 626  VLTRKLVKGIFMPMIYGKTLMSTASDLKDTLSRFITHKECFDVASVCFKFWRTQYQNTEC 685

Query: 919  LIRLIRNIGWIASACERPVYESVPDFKTVQETMKMEPNNIWVYDKLHKKKRRVTLRVASS 978
            LIRLIR+IGWIASA + PV+  VP F TVQ+ MKM+P N+W YD LHKK+RRVTLRV+SS
Sbjct: 686  LIRLIRHIGWIASARDSPVFYRVPSFTTVQDYMKMDPINVWFYDGLHKKRRRVTLRVSSS 745

Query: 979  KRDRRKTAISTFVNFIHQRDASIAMKVVEFMQHRGAPIYTVHDNFITTAEYSEFLPDYYS 1028
            KRDRRKT ISTFVNFIHQRDA IAMKVVE M  +GAPIYTVHDNFITTAEYS FLP  Y 
Sbjct: 746  KRDRRKTEISTFVNFIHQRDAHIAMKVVECMLEKGAPIYTVHDNFITTAEYSYFLPIIYI 805

BLAST of Sgr022489 vs. ExPASy TrEMBL
Match: A0A6J5VXK4 (DNA-directed RNA polymerase OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS3296 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 7.2e-231
Identity = 482/894 (53.91%), Postives = 564/894 (63.09%), Query Frame = 0

Query: 259  RFNNEFDSLFL-NQNEEEDIFKSQSAQYKDKVFDILNNGKARLPLSDEELYNIQMEIEDL 318
            RF NEF +  L N N+     KS S QYK+  FDIL   K RLPLS +EL  IQ +IE++
Sbjct: 26   RFWNEFYTYLLQNTNKSGQHGKSHSVQYKETAFDILEEAKTRLPLSVDELSEIQKQIEEI 85

Query: 319  TMTFDEESM--------------NEKC--------KLSEYDTGLLSEFGQYTIEALIVYV 378
            T+ FDE+S+              NE+            + D  LLSEFGQYTIEALIV+V
Sbjct: 86   TIWFDEKSIFRSASDIIKILIRENEESAKDFLRRRPFEKDDLELLSEFGQYTIEALIVHV 145

Query: 379  LCMVFTLPYNENLMIRLASLVDQLDTNVRSQALLLKSRRCKQSFSTVREE--VKKSGSGD 438
                                   L ++VR QA LLKS RC + FS+   +  VKKSG   
Sbjct: 146  -----------------------LKSSVRHQASLLKSGRCNKPFSSATNDFKVKKSGKDR 205

Query: 439  K-------YTIGSKLVSFMVERELIKLISCDLSGSVRAIKKKKESYFLPSHLYAVCNFDI 498
            K       Y  GS LV FM ER+LI L++ DLSG+VR +KKKK SYFLPSHLYAVCNFDI
Sbjct: 206  KRSKLVMMYPFGSGLVQFMEERKLISLVT-DLSGTVR-VKKKKGSYFLPSHLYAVCNFDI 265

Query: 499  SLLPIKFNLPMVYKPVDWVCARGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLSSGDINH 558
            SLLPIK NLPMV KP DW  A      PR+LSDLSGGYLSG TG +Y+RYRLLSSGDINH
Sbjct: 266  SLLPIKLNLPMVCKPRDWTSACRGDQNPRYLSDLSGGYLSGPTGGLYDRYRLLSSGDINH 325

Query: 559  FYIDI--ENRYEDLCGVMNKLQHQAFQINT------------------------------ 618
            FYIDI  E  YE LC VMNKLQ QAFQIN+                              
Sbjct: 326  FYIDIGREKNYEKLCLVMNKLQGQAFQINSHWLKCLKYNEDSFVESGLLMPRFLSSMNIK 385

Query: 619  ----------------------------------------------LAYDGYQFDLPAFL 678
                                                           AY+GY F LPAFL
Sbjct: 386  DVSNLLREFHMKDEVINKLCNFSELLHTLSKNIQRSRYENLIMKLAQAYEGYHFYLPAFL 445

Query: 679  DFRGRIYRCGILHFHERDLARSLIIFADKKDEDIKLSVGNNFLPKSFFAATAFHDQSFDS 738
            DFRGRIYR G+LHFHERDLARS+I+FAD K          N    ++ AA AFH +SF S
Sbjct: 446  DFRGRIYRSGVLHFHERDLARSMIVFADIKS-------SGNIDMNAYLAAAAFHYKSFVS 505

Query: 739  VDDATSWFCNNMMLPQISDHQILSYTLDAKRPFQFLANMI--TSNR----DQIPITQDAS 798
            VD+A  +F NN  L    D  +L Y  +AKRPFQ  A++I  TS       +IP+TQDAS
Sbjct: 506  VDEAL-YFSNNNFLQLSHDDDLLMYAREAKRPFQLFAHLIGVTSPNLKVITRIPLTQDAS 565

Query: 799  ASAYQIMSDFLLDETLAKRTNLIPSSDKKIQDIYSLRLEELKEFIVAELEDKNRSMLVCE 858
            ASAYQIMS FLLDE+LA RTNLIPS D KIQD+YS  LE+LK F+ AEL++ + S +VC 
Sbjct: 566  ASAYQIMSYFLLDESLASRTNLIPSLDGKIQDVYSFILEDLKVFMKAELDNNHLSTIVCN 625

Query: 859  LFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIASVCFKFWRTKYHHMEC 918
            + TRKLVK IFMP+IYGKTLMS A DLKD LS +IT KE F +ASVCFKFWRT+Y + EC
Sbjct: 626  VLTRKLVKGIFMPMIYGKTLMSTASDLKDTLSRFITHKECFDVASVCFKFWRTQYQNTEC 685

Query: 919  LIRLIRNIGWIASACERPVYESVPDFKTVQETMKMEPNNIWVYDKLHKKKRRVTLRVASS 978
            LIRLIR+IGWIASA + PV+  VP F TVQ+ MKM+P N+W YD LHKK+RRVTLRV+SS
Sbjct: 686  LIRLIRHIGWIASARDSPVFYRVPSFTTVQDYMKMDPINVWFYDGLHKKRRRVTLRVSSS 745

Query: 979  KRDRRKTAISTFVNFIHQRDASIAMKVVEFMQHRGAPIYTVHDNFITTAEYSEFLPDYYS 1028
            KRDRRKT ISTFVNFIHQRDA IAMKVVE M  +GAPIYTVHDNFITTAEYS FLP  Y 
Sbjct: 746  KRDRRKTEISTFVNFIHQRDAHIAMKVVECMLEKGAPIYTVHDNFITTAEYSYFLPIIYI 805

BLAST of Sgr022489 vs. ExPASy TrEMBL
Match: A0A6B9XVF7 (DNA-directed RNA polymerase OS=Picea sitchensis OX=3332 GN=ymfrpo PE=3 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 1.7e-216
Identity = 437/792 (55.18%), Postives = 511/792 (64.52%), Query Frame = 0

Query: 359  VFTLPYNENLMIRLASLVDQLDTNVRSQALLLKSRRCKQSFSTVREE-----------VK 418
            +F      N +IR+ASLV+QL+++VR QA L KS RC + FS+   +           VK
Sbjct: 1    MFFYSVESNSLIRVASLVEQLESSVRHQASLFKSGRCNKPFSSATNDFKVKKSGNDFKVK 60

Query: 419  KSGSGDK-------YTIGSKLVSFMVERELIKLISCDLSGSVRAIKKKKESYFLPSHLYA 478
            KSG   K       Y  GS LV FM ER+LI L++ DLSG+VR ++KKK SYFLPSHLYA
Sbjct: 61   KSGKDRKSSKLVMMYPFGSGLVQFMEERKLISLVT-DLSGTVR-VEKKKGSYFLPSHLYA 120

Query: 479  VCNFDISLLPIKFNLPMVYKPVDWVCARGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLS 538
            VCNFDISLLPIK NLPMV KP DW  A      PR+LSDLSGGYLSG TG +Y+RYRLLS
Sbjct: 121  VCNFDISLLPIKLNLPMVCKPRDWTSACRGDQNPRYLSDLSGGYLSGPTGGLYDRYRLLS 180

Query: 539  SGDINHFYIDI--ENRYEDLCGVMNKLQHQAFQINTL----------------------- 598
            SGDINHFYIDI  EN YE LC VMNK Q QAFQIN+                        
Sbjct: 181  SGDINHFYIDIGRENNYEKLCVVMNKQQGQAFQINSYWFQFLQDNEDSFVESGLLMPRFL 240

Query: 599  -----------------------------------------------------AYDGYQF 658
                                                                 AYDGY F
Sbjct: 241  SSMNIKDVSNLLREFHMKDEVIKKLCSFSELLRTLSKNIQRSRYENLIMKLAQAYDGYHF 300

Query: 659  DLPAFLDFRGRIYRCGILHFHERDLARSLIIFADKKDEDIKLSVGNNFLPKSFFAATAFH 718
            DLPAFLDFRGRIYR G+LHFHERDLARSLI+FAD K +            K+ F A AFH
Sbjct: 301  DLPAFLDFRGRIYRSGVLHFHERDLARSLIVFADLKSQGTSDD-------KTNFEAAAFH 360

Query: 719  DQSFDSVDDATSWFCNNMMLPQISDHQILSYTLDAKRPFQFLAN---MITSNR---DQIP 778
             +SFDSVDD   WF N+    Q     +  Y  +AKRPFQF A+   +++ N      IP
Sbjct: 361  YKSFDSVDDGLDWFSND-FFSQRGSPDVFMYAREAKRPFQFHAHLMALVSKNMKLISSIP 420

Query: 779  ITQDASASAYQIMSDFLLDETLAKRTNLIPSSDKKIQDIYSLRLEELKEFIVAELEDKNR 838
             TQDASASA+QI+S FLLD  LA RTNLIPS D KIQD+YS  LE+LK F++AE+E+KN 
Sbjct: 421  FTQDASASAFQILSYFLLDAPLASRTNLIPSLDGKIQDVYSYILEDLKVFMIAEMENKNL 480

Query: 839  SMLVCELFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIASVCFKFWRTK 898
            S +VC   TRKLVK IFMP+IYGKTLMS A DLKDHLS +IT KESF +ASVCFKFWRT+
Sbjct: 481  STIVCNQLTRKLVKGIFMPMIYGKTLMSTARDLKDHLSHFITHKESFEVASVCFKFWRTQ 540

Query: 899  YHHMECLIRLIRNIGWIASACERPVYESVPDFKTVQETMKMEPNNIWVYDKLHKKKRRVT 958
            +  MECLIRLIR+IGWIASA +RPV+  VP F TVQ+ M M P NIW YD LHKK+ RVT
Sbjct: 541  FPGMECLIRLIRHIGWIASARDRPVFYRVPYFTTVQDYMIMVPINIWFYDGLHKKRHRVT 600

Query: 959  LRVASSKRDRRKTAISTFVNFIHQRDASIAMKVVEFMQHRGAPIYTVHDNFITTAEYSEF 1018
            LRV+SSKRDRRKT ISTFVNFIHQRDA IAMKVVE M  +GAPIYTVHDNFITTAEY+  
Sbjct: 601  LRVSSSKRDRRKTDISTFVNFIHQRDAHIAMKVVERMLEKGAPIYTVHDNFITTAEYTHD 660

Query: 1019 LPDYYSSTICEMGPPLQILNEFIHMNVIQPIV----GGRPESHFTCNGFSLNMLHSYLMA 1040
            LP  Y   IC+MGPPL ILNEFI+MN+IQPIV     G  E +F     S  +LH YL A
Sbjct: 661  LPRIYIKVICDMGPPLSILNEFIYMNIIQPIVKVESDGPHEGYFADKVISKEILHYYLKA 720

BLAST of Sgr022489 vs. ExPASy TrEMBL
Match: A0A101M3N5 (DNA-directed RNA polymerase OS=Picea glauca OX=3330 GN=rpo PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 8.6e-216
Identity = 436/792 (55.05%), Postives = 510/792 (64.39%), Query Frame = 0

Query: 359  VFTLPYNENLMIRLASLVDQLDTNVRSQALLLKSRRCKQSFSTVREE-----------VK 418
            +F      N +IR+ASLV+QL+++VR QA L KS RC + FS+   +           VK
Sbjct: 1    MFFYSVESNSLIRVASLVEQLESSVRHQASLFKSGRCNKPFSSATNDFKVKKSGNDFKVK 60

Query: 419  KSGSGDK-------YTIGSKLVSFMVERELIKLISCDLSGSVRAIKKKKESYFLPSHLYA 478
            KSG   K       Y  GS LV FM ER+LI L++ DLSG+VR ++KKK SYFLPSHLYA
Sbjct: 61   KSGKDRKSSKLVMMYPFGSGLVQFMEERKLISLVT-DLSGTVR-VEKKKGSYFLPSHLYA 120

Query: 479  VCNFDISLLPIKFNLPMVYKPVDWVCARGIGHKPRHLSDLSGGYLSGLTGEIYNRYRLLS 538
            VCNFDISLLPIK NLPMV KP DW  A      PR+LSDLSGGYLSG TG +Y+RYRLLS
Sbjct: 121  VCNFDISLLPIKLNLPMVCKPRDWTSACRGDQNPRYLSDLSGGYLSGPTGGLYDRYRLLS 180

Query: 539  SGDINHFYIDI--ENRYEDLCGVMNKLQHQAFQINTL----------------------- 598
            SGDINHFYIDI  E  YE LC VMNK Q QAFQIN+                        
Sbjct: 181  SGDINHFYIDIGREKNYEKLCVVMNKQQGQAFQINSYWFQFLQDNEDSFVESGLLMPRFL 240

Query: 599  -----------------------------------------------------AYDGYQF 658
                                                                 AYDGY F
Sbjct: 241  SSMNIKDVSNLLREFHMKDEVIKKLCSFSELLRTLSKNIQRSRYENLIMKLAQAYDGYHF 300

Query: 659  DLPAFLDFRGRIYRCGILHFHERDLARSLIIFADKKDEDIKLSVGNNFLPKSFFAATAFH 718
            DLPAFLDFRGRIYR G+LHFHERDLARSLI+FAD K +            K+ F A AFH
Sbjct: 301  DLPAFLDFRGRIYRSGVLHFHERDLARSLIVFADLKSQGTSDD-------KTNFEAAAFH 360

Query: 719  DQSFDSVDDATSWFCNNMMLPQISDHQILSYTLDAKRPFQFLAN---MITSNR---DQIP 778
             +SFDSVDD   WF N+    Q     +  Y  +AKRPFQF A+   +++ N      IP
Sbjct: 361  YKSFDSVDDGLDWFSND-FFSQRGSPDVFMYAREAKRPFQFHAHLMALVSKNMKLISSIP 420

Query: 779  ITQDASASAYQIMSDFLLDETLAKRTNLIPSSDKKIQDIYSLRLEELKEFIVAELEDKNR 838
             TQDASASA+QI+S FLLD  LA RTNLIPS D KIQD+YS  LE+LK F++AE+E+KN 
Sbjct: 421  FTQDASASAFQILSYFLLDAPLASRTNLIPSLDGKIQDVYSYILEDLKVFMIAEMENKNL 480

Query: 839  SMLVCELFTRKLVKSIFMPIIYGKTLMSIAGDLKDHLSIYITFKESFIIASVCFKFWRTK 898
            S +VC   TRKLVK IFMP+IYGKTLMS A DLKDHLS +IT KESF +ASVCFKFWRT+
Sbjct: 481  STIVCNQLTRKLVKGIFMPMIYGKTLMSTARDLKDHLSHFITHKESFEVASVCFKFWRTQ 540

Query: 899  YHHMECLIRLIRNIGWIASACERPVYESVPDFKTVQETMKMEPNNIWVYDKLHKKKRRVT 958
            +  MECLIRLIR+IGWIASA +RPV+  VP F TVQ+ M M P NIW YD LHKK+ RVT
Sbjct: 541  FPGMECLIRLIRHIGWIASARDRPVFYRVPYFTTVQDYMIMVPINIWFYDGLHKKRHRVT 600

Query: 959  LRVASSKRDRRKTAISTFVNFIHQRDASIAMKVVEFMQHRGAPIYTVHDNFITTAEYSEF 1018
            LRV+SSKRDRRKT ISTFVNFIHQRDA IAMKVVE M  +GAPIYTVHDNFITTAEY+  
Sbjct: 601  LRVSSSKRDRRKTDISTFVNFIHQRDAHIAMKVVERMLEKGAPIYTVHDNFITTAEYTHD 660

Query: 1019 LPDYYSSTICEMGPPLQILNEFIHMNVIQPIV----GGRPESHFTCNGFSLNMLHSYLMA 1040
            LP  Y   IC+MGPPL ILNEFI+MN+IQPIV     G  E +F     S  +LH YL A
Sbjct: 661  LPRIYIKVICDMGPPLSILNEFIYMNIIQPIVKVESDGPHEGYFADKVISKEILHYYLKA 720