Sgr022361 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr022361
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionARF guanine-nucleotide exchange factor GNL2
Locationtig00154107: 905425 .. 907422 (+)
RNA-Seq ExpressionSgr022361
SyntenySgr022361
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAACGATCAGACGAGGAAGATGACAAGGAGTCCTCCAAAACCAAGCGGAGGGAGCTAGGGCTCTCCTGCATGTTGAATACCGAAGTCGGCGCCGTACTCGCCGTCATCCGCCGTCCATCATCGGAATTGAATTCGCAACACATTTCTCCCATCGACGACACTTTCGATTCCTCGATCCAACAATCCTTGAAATCCCTTCGAGCTCTCATATTTCATCCTCAGCAGAAATGGCGAACCATCGACCCGTCGATCTACATCTCTCCATTCCTCGACGTGATACAGAGCGACGACATCCCGGCGGCAGCCACCGGCGTGGCTCTCTCGGCCTTACTCAAGATAATAAGGTCGAGATCTTCGACGCGAAACTCCTGGAGCCAAAGACGCAATCAATTAATAGTGTTGGGAATTACGAACTGTAAATTGGAAAAAACTGACCTCGTTACCGAAGACGCCGTGATGATGAAGATTCTGCAGGTTCTTGCCGAACTCATGAACCACCGGGCATCATACCTGCTAAACGATCAATCAGTCTGCACCATTGTAAACACCTGCTTCAATGTGGTTCAACAATCAGCGAGCAGAGGCGATTTGCTCCAACGAACCGCGAGGTACACGATGCATGAGCTGATTCAGATCATCTTCTCCCGATTGCCTGAAATAGAGGTTAGAGATGGAGAAGATTCAGAATCAGATACAGAAGATGCAGATTTAGGCGGCAGTTTGGATTCAGGATACGGAATTAGGTGTGTGATCGATGTGTTTCACTTCCTCTGCTCGCTGCTCAACGTCGTGGAAATGGTGGAGGTGGATGGATCGACTTCGCGCACTGCGGACGAAGACGTTCAGCTGTTTGCACTCGTTCTGATAAACTCGGCGGTGGAGTTGAGCGGAGACGCGATCGGGAAGCATCCGAAGTTGTTGAGGATGGTTCAGGATGATCTGTTTCACCATCTCATTCACTACGGCGCCTGTTCGAATCCGCTGGTTCTGTCCATGATCTGCAGTACAGTTTTGAATGTCTATCACTTCCTTCGCAGGTACGAGGCTTCCCTCGCTTCGCTATATATATAATTTTTAAAAATTAAGACTATTGTTTTTTCTTTAAATTAAAAAAAAAAAAAAAATCAAGGTGAGAGGATTTAAAACTTCCATGAACTCTCTACACTTTTTTTTCCTCTAGAATATGAACTCTCTACTTTTGATAGTTGAATTGCTACAAAATTTGATAATAATAATATTGTAAATTATTATTTGTAATAGAAATTAAAAGAATAAAAATCGAGTTTTAAAGAGGCATTTGGCTCACAAGTTTAAAATTGTAAATTGAAGTTACGAGATATAAAATTTATTTATTTTAAATTATGAATTTGTCAATCACATGAACTACATGTACTTAAGCTACAAACACCGTACAGATCACAAATTAAATTAAAGTGACATAAATCTAAATTTTTGCTTCATGATTCAAGACACAGGCATCTCAACACAGAATGAAGGCTAATTAAAACCATGCCCCAAATATGAGACGAAAATACCATAATAGAAGTGATTAACCAGAATGAAAAATTGAAAAAAGGGAAAAAAAAACTCCTAGACAATAACAATATGCAACCTTTATTTCATTAAGGAAATAAGAAAAATCATTTCTACGCTATTTTTTTTGCTACAAAATATCCTTACAATTTTCCAGGATTTGAGAGGATTGGTCCATCTAAAAGAAATGATATATTTTTTTTCCCTCATTTTTTGGGCAGGTTTGTTCGACTTCAATTAGAAGCCTTCTTTGTTTACGTGGCATTGAGATTAGCATCTTTTGGAAACTCAACCCAAATCCAAGAAGTTGCACTAGAAGGCATTATCAACTTCTGCAGGCAGTCCTCCTTCATTCTTGAAATATACGTAAACTACGACTGCGATCCCCTTCGTTGGAATCTGTTCGAGGAGATCGGGAAGTTGCTATGTAAGCTCTCGTTTCCGTGGGGAGCCCCTTGA

mRNA sequence

ATGGAGAAACGATCAGACGAGGAAGATGACAAGGAGTCCTCCAAAACCAAGCGGAGGGAGCTAGGGCTCTCCTGCATGTTGAATACCGAAGTCGGCGCCGTACTCGCCGTCATCCGCCGTCCATCATCGGAATTGAATTCGCAACACATTTCTCCCATCGACGACACTTTCGATTCCTCGATCCAACAATCCTTGAAATCCCTTCGAGCTCTCATATTTCATCCTCAGCAGAAATGGCGAACCATCGACCCGTCGATCTACATCTCTCCATTCCTCGACGTGATACAGAGCGACGACATCCCGGCGGCAGCCACCGGCGTGGCTCTCTCGGCCTTACTCAAGATAATAAGGTCGAGATCTTCGACGCGAAACTCCTGGAGCCAAAGACGCAATCAATTAATAGTGTTGGGAATTACGAACTGTAAATTGGAAAAAACTGACCTCGTTACCGAAGACGCCGTGATGATGAAGATTCTGCAGGTTCTTGCCGAACTCATGAACCACCGGGCATCATACCTGCTAAACGATCAATCAGTCTGCACCATTGTAAACACCTGCTTCAATGTGGTTCAACAATCAGCGAGCAGAGGCGATTTGCTCCAACGAACCGCGAGGTACACGATGCATGAGCTGATTCAGATCATCTTCTCCCGATTGCCTGAAATAGAGGTTAGAGATGGAGAAGATTCAGAATCAGATACAGAAGATGCAGATTTAGGCGGCAGTTTGGATTCAGGATACGGAATTAGGTGTGTGATCGATGTGTTTCACTTCCTCTGCTCGCTGCTCAACGTCGTGGAAATGGTGGAGGTGGATGGATCGACTTCGCGCACTGCGGACGAAGACGTTCAGCTGTTTGCACTCGTTCTGATAAACTCGGCGGTGGAGTTGAGCGGAGACGCGATCGGGAAGCATCCGAAGTTGTTGAGGATGGTTCAGGATGATCTGTTTCACCATCTCATTCACTACGGCGCCTGTTCGAATCCGCTGGTTCTGTCCATGATCTGCAGTACAGTTTTGAATGTCTATCACTTCCTTCGCAGGTTTGTTCGACTTCAATTAGAAGCCTTCTTTGTTTACGTGGCATTGAGATTAGCATCTTTTGGAAACTCAACCCAAATCCAAGAAGTTGCACTAGAAGGCATTATCAACTTCTGCAGGCAGTCCTCCTTCATTCTTGAAATATACGTAAACTACGACTGCGATCCCCTTCGTTGGAATCTGTTCGAGGAGATCGGGAAGTTGCTATGTAAGCTCTCGTTTCCGTGGGGAGCCCCTTGA

Coding sequence (CDS)

ATGGAGAAACGATCAGACGAGGAAGATGACAAGGAGTCCTCCAAAACCAAGCGGAGGGAGCTAGGGCTCTCCTGCATGTTGAATACCGAAGTCGGCGCCGTACTCGCCGTCATCCGCCGTCCATCATCGGAATTGAATTCGCAACACATTTCTCCCATCGACGACACTTTCGATTCCTCGATCCAACAATCCTTGAAATCCCTTCGAGCTCTCATATTTCATCCTCAGCAGAAATGGCGAACCATCGACCCGTCGATCTACATCTCTCCATTCCTCGACGTGATACAGAGCGACGACATCCCGGCGGCAGCCACCGGCGTGGCTCTCTCGGCCTTACTCAAGATAATAAGGTCGAGATCTTCGACGCGAAACTCCTGGAGCCAAAGACGCAATCAATTAATAGTGTTGGGAATTACGAACTGTAAATTGGAAAAAACTGACCTCGTTACCGAAGACGCCGTGATGATGAAGATTCTGCAGGTTCTTGCCGAACTCATGAACCACCGGGCATCATACCTGCTAAACGATCAATCAGTCTGCACCATTGTAAACACCTGCTTCAATGTGGTTCAACAATCAGCGAGCAGAGGCGATTTGCTCCAACGAACCGCGAGGTACACGATGCATGAGCTGATTCAGATCATCTTCTCCCGATTGCCTGAAATAGAGGTTAGAGATGGAGAAGATTCAGAATCAGATACAGAAGATGCAGATTTAGGCGGCAGTTTGGATTCAGGATACGGAATTAGGTGTGTGATCGATGTGTTTCACTTCCTCTGCTCGCTGCTCAACGTCGTGGAAATGGTGGAGGTGGATGGATCGACTTCGCGCACTGCGGACGAAGACGTTCAGCTGTTTGCACTCGTTCTGATAAACTCGGCGGTGGAGTTGAGCGGAGACGCGATCGGGAAGCATCCGAAGTTGTTGAGGATGGTTCAGGATGATCTGTTTCACCATCTCATTCACTACGGCGCCTGTTCGAATCCGCTGGTTCTGTCCATGATCTGCAGTACAGTTTTGAATGTCTATCACTTCCTTCGCAGGTTTGTTCGACTTCAATTAGAAGCCTTCTTTGTTTACGTGGCATTGAGATTAGCATCTTTTGGAAACTCAACCCAAATCCAAGAAGTTGCACTAGAAGGCATTATCAACTTCTGCAGGCAGTCCTCCTTCATTCTTGAAATATACGTAAACTACGACTGCGATCCCCTTCGTTGGAATCTGTTCGAGGAGATCGGGAAGTTGCTATGTAAGCTCTCGTTTCCGTGGGGAGCCCCTTGA

Protein sequence

MEKRSDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDSSIQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSRSSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDADLGGSLDSGYGIRCVIDVFHFLCSLLNVVEMVEVDGSTSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFFVYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLLCKLSFPWGAP
Homology
BLAST of Sgr022361 vs. NCBI nr
Match: XP_022157565.1 (ARF guanine-nucleotide exchange factor GNL2 [Momordica charantia])

HSP 1 Score: 737.6 bits (1903), Expect = 5.9e-209
Identity = 382/428 (89.25%), Postives = 402/428 (93.93%), Query Frame = 0

Query: 1   MEKRSDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDSS 60
           M KRSDEEDD++SSK+KRRELGLSCMLNTEVGA+LAVIRRP SELNS +ISPIDDTFDSS
Sbjct: 1   MTKRSDEEDDRDSSKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYISPIDDTFDSS 60

Query: 61  IQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR- 120
           IQQSLKSLRALIFHPQQKWRTIDPSIYISP LDV+QSDDIPAAATGVALSALLKII+   
Sbjct: 61  IQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVVQSDDIPAAATGVALSALLKIIKVEI 120

Query: 121 SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSV 180
            + +   ++    LIVLGITNCKLEKTDL+TEDAVMMKILQVLA LMNHRASYLLNDQSV
Sbjct: 121 FNEKTPGAKDAINLIVLGITNCKLEKTDLITEDAVMMKILQVLAGLMNHRASYLLNDQSV 180

Query: 181 CTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDADL 240
           CTIVNTCFNVVQQSA+RGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDADL
Sbjct: 181 CTIVNTCFNVVQQSANRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDADL 240

Query: 241 GGSLDSGYGIRCVIDVFHFLCSLLNVVEMVE-VDGSTSRTADEDVQLFALVLINSAVELS 300
           GGSLDSGYGIRCVIDVFHFLCSL+NVVEM E +DG  SRTADEDVQLFALVLINSAVELS
Sbjct: 241 GGSLDSGYGIRCVIDVFHFLCSLMNVVEMGEMMDGLASRTADEDVQLFALVLINSAVELS 300

Query: 301 GDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFF 360
           GDAIGKHP+LLRMVQDDLFHHLIHYGA  NPLVLSMICSTVLN+YHFLRRFVRLQLEAFF
Sbjct: 301 GDAIGKHPRLLRMVQDDLFHHLIHYGARCNPLVLSMICSTVLNIYHFLRRFVRLQLEAFF 360

Query: 361 VYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLLCK 420
           VYVALR AS+GNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNLFE+IGKLLCK
Sbjct: 361 VYVALRSASYGNSTQIQEVALEGIINFCRQSSFILETYVNYDCDPLRWNLFEDIGKLLCK 420

Query: 421 LSFPWGAP 427
           LSFP G+P
Sbjct: 421 LSFPAGSP 428

BLAST of Sgr022361 vs. NCBI nr
Match: XP_038889761.1 (ARF guanine-nucleotide exchange factor GNL2 [Benincasa hispida])

HSP 1 Score: 729.6 bits (1882), Expect = 1.6e-206
Identity = 384/434 (88.48%), Postives = 402/434 (92.63%), Query Frame = 0

Query: 1   MEKR-SDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDS 60
           MEKR SD+E++K+S K+KRRELGLSCMLNTEVGAVLAVIRRP S+LNS +IS IDDT+DS
Sbjct: 1   MEKRSSDDEEEKDSPKSKRRELGLSCMLNTEVGAVLAVIRRPPSDLNSPYISTIDDTYDS 60

Query: 61  SIQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR 120
           SIQQSLKSLRALIFHPQQKWRTIDPSIYISP LDVIQSDDIPAAATGVALSALLKII+  
Sbjct: 61  SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121 -SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQS 180
             + +    +    LIVLGITNCKLEKTDLVTEDAVMMKILQVLA LMNHRASYLLNDQS
Sbjct: 121 IFNEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKILQVLAGLMNHRASYLLNDQS 180

Query: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDAD 240
           VCTIVNTCFNVVQQSASRGDLLQRTARYTM+ELIQIIFSRLPEIEVRDGEDSESDTEDAD
Sbjct: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEDSESDTEDAD 240

Query: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMVEVD------GSTSRTADEDVQLFALVLIN 300
           LGGSLDSGYGIRCVIDVFHFLCSLLNVVE+VE+       G  SRTADEDVQLFALVLIN
Sbjct: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIVEIGDGGLGMGGGSRTADEDVQLFALVLIN 300

Query: 301 SAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRL 360
           SAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGA SNPLVLSMICSTVLN+YHFLRRFVRL
Sbjct: 301 SAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGASSNPLVLSMICSTVLNIYHFLRRFVRL 360

Query: 361 QLEAFFVYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEI 420
           QLEAFFVYVAL+LASFGNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNLFEEI
Sbjct: 361 QLEAFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEI 420

Query: 421 GKLLCKLSFPWGAP 427
           GKLLCKLSFP G+P
Sbjct: 421 GKLLCKLSFPTGSP 434

BLAST of Sgr022361 vs. NCBI nr
Match: KAA0061562.1 (ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo var. makuwa] >TYK10710.1 ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo var. makuwa])

HSP 1 Score: 723.4 bits (1866), Expect = 1.1e-204
Identity = 378/430 (87.91%), Postives = 400/430 (93.02%), Query Frame = 0

Query: 1   MEKR-SDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDS 60
           M+KR SD+ED+K+S K+KRRELGLSCMLNTEVGA+LAVIRRP SELNS +I+ ID+T+DS
Sbjct: 1   MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 60

Query: 61  SIQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR 120
           +IQQSLKSLRALIFHPQQKWRTIDPSIYISP LDVIQSDDIPAAATGVALSALLKII+  
Sbjct: 61  TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121 -SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQS 180
               +    +    LIVLGITNCKLEKTDLVTEDAVMMK+LQVLA LMNHRASYLLNDQS
Sbjct: 121 IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDAD 240
           VCTIVNTCFNVVQQSASRGDLLQRTARYTM+ELIQIIFSRLPE+EVRDGE+SESDTEDAD
Sbjct: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 240

Query: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMVEV--DGSTSRTADEDVQLFALVLINSAVE 300
           LGGS+DSGYGIRCVIDVFHFLCSLLNVVEMVEV   G  SRTADEDVQLFALVLINSAVE
Sbjct: 241 LGGSMDSGYGIRCVIDVFHFLCSLLNVVEMVEVGDGGLGSRTADEDVQLFALVLINSAVE 300

Query: 301 LSGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEA 360
           LSGDAIGKHPKLLRMVQDDLFHHLIHYGA SNPLVLSMICSTVLN+YHFLRRFVRLQLEA
Sbjct: 301 LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360

Query: 361 FFVYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLL 420
           F +YVALRLASFGNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNLFEEIGKLL
Sbjct: 361 FSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420

Query: 421 CKLSFPWGAP 427
           CKLSFP G+P
Sbjct: 421 CKLSFPTGSP 430

BLAST of Sgr022361 vs. NCBI nr
Match: XP_008458277.1 (PREDICTED: ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo])

HSP 1 Score: 722.2 bits (1863), Expect = 2.6e-204
Identity = 377/430 (87.67%), Postives = 400/430 (93.02%), Query Frame = 0

Query: 1   MEKR-SDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDS 60
           M+KR SD+ED+K+S K+KRRELGLSCMLNTEVGA+LAVIRRP SELNS +I+ ID+T+DS
Sbjct: 3   MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 62

Query: 61  SIQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR 120
           +IQQSLKSLRALIFHPQQKWRTIDPSIYISP LDVIQSDDIPAAATGVALSALLKII+  
Sbjct: 63  TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 122

Query: 121 -SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQS 180
               +    +    LIVLGITNCKLEKTDLVTEDAVMMK+LQVLA LMNHRASYLLNDQS
Sbjct: 123 IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 182

Query: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDAD 240
           VCTIVNTCFNVVQQSASRGDLLQRTARYTM+ELIQIIFSRLPE+EVRDGE+SESDTEDAD
Sbjct: 183 VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 242

Query: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMVEV--DGSTSRTADEDVQLFALVLINSAVE 300
           LGGS+DSGYGIRCVIDVFHFLCSLLNVVEMVEV   G  SRTADEDVQLFALVLINSAVE
Sbjct: 243 LGGSMDSGYGIRCVIDVFHFLCSLLNVVEMVEVGDGGLGSRTADEDVQLFALVLINSAVE 302

Query: 301 LSGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEA 360
           LSGDAIGKHPKLLRMVQDDLFHHLIHYGA SNPLVLSMICSTVLN+YHFLRRFVRLQLEA
Sbjct: 303 LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 362

Query: 361 FFVYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLL 420
           F +YVALRLASFGNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNLFEE+GKLL
Sbjct: 363 FSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEMGKLL 422

Query: 421 CKLSFPWGAP 427
           CKLSFP G+P
Sbjct: 423 CKLSFPTGSP 432

BLAST of Sgr022361 vs. NCBI nr
Match: XP_004139429.1 (ARF guanine-nucleotide exchange factor GNL2 [Cucumis sativus] >KGN60625.1 hypothetical protein Csa_019390 [Cucumis sativus])

HSP 1 Score: 719.9 bits (1857), Expect = 1.3e-203
Identity = 378/431 (87.70%), Postives = 401/431 (93.04%), Query Frame = 0

Query: 1   MEKR-SDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDS 60
           M+KR SD+ED+K+S K+KRRELGLSCMLNTEVG++LAVIRRP SELNS +IS ID+T+DS
Sbjct: 1   MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60

Query: 61  SIQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR 120
           +IQQSLKSLRALIFHPQQKWRTIDPSIYISP LDVIQSDDIPAAATGVALSALLKII+  
Sbjct: 61  TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121 -SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQS 180
               +   ++    LIV+GITNCKLEKTDLVTEDAVMMK+LQVLA LMNHRASYLLNDQS
Sbjct: 121 IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDAD 240
           VCTIVNTCFNVVQQSASRGDLLQRTARYTM+ELIQIIFSRLPEIEVRDGE+SESDTEDAD
Sbjct: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240

Query: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVE-MVEV--DGSTSRTADEDVQLFALVLINSAV 300
           LGGSLDSGYGIRCVIDVFHFLCSLLNVVE MVEV   G  SRTADEDVQLFALVLINSAV
Sbjct: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300

Query: 301 ELSGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLE 360
           ELSGDAIGKHPKLLRMVQDDLFHHLIHYGA SNPLVLSMICSTVLN+YHFLRRFVRLQLE
Sbjct: 301 ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360

Query: 361 AFFVYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKL 420
           +FFVYVAL+LASFGNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNL EEIGKL
Sbjct: 361 SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420

Query: 421 LCKLSFPWGAP 427
           LCKLSFP G+P
Sbjct: 421 LCKLSFPTGSP 431

BLAST of Sgr022361 vs. ExPASy Swiss-Prot
Match: F4K2K3 (ARF guanine-nucleotide exchange factor GNL2 OS=Arabidopsis thaliana OX=3702 GN=GNL2 PE=2 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 3.7e-158
Identity = 290/423 (68.56%), Postives = 348/423 (82.27%), Query Frame = 0

Query: 10  DKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPID-DTFDSSIQQSLKSL 69
           D+ + + KR+ELG+SCMLNTEVGAVLAVIRRP SE    ++SP + D  DSS+QQSLKSL
Sbjct: 2   DRIAVRAKRKELGISCMLNTEVGAVLAVIRRPLSE---SYLSPQETDHCDSSVQQSLKSL 61

Query: 70  RALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR-SSTRNSWS 129
           RALIF+PQQ WRTIDPS+Y+SPFL+VIQSD+IPA+AT VALS++LKI++      +   +
Sbjct: 62  RALIFNPQQDWRTIDPSVYLSPFLEVIQSDEIPASATAVALSSILKILKIEIFDEKTPGA 121

Query: 130 QRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSVCTIVNTCF 189
           +     IV GIT+C+LEKTDLV+EDAVMM+ILQVL  +M H +S LL DQ+VCTIVNTCF
Sbjct: 122 KDAMNSIVSGITSCRLEKTDLVSEDAVMMRILQVLTGIMKHPSSELLEDQAVCTIVNTCF 181

Query: 190 NVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVR---DGEDSESDTEDADLGGSLD 249
            VVQQS  RGDLLQR  RYTMHELIQIIFSRLP+ EVR    GEDSESDT++ D+ G   
Sbjct: 182 QVVQQSTGRGDLLQRNGRYTMHELIQIIFSRLPDFEVRGDEGGEDSESDTDEIDMSG--- 241

Query: 250 SGYGIRCVIDVFHFLCSLLNVVEMVE-VDGSTSRTADEDVQLFALVLINSAVELSGDAIG 309
            GYGIRC ID+FHFLCSLLNVVE+VE ++G+   TADEDVQ+FALVLINSA+ELSGDAIG
Sbjct: 242 -GYGIRCCIDIFHFLCSLLNVVEVVENLEGTNVHTADEDVQIFALVLINSAIELSGDAIG 301

Query: 310 KHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFFVYVAL 369
           +HPKLLRMVQDDLFHHLIHYGA S+PLVLSMICS +LN+YHFLR+F+RLQLEAFF +V L
Sbjct: 302 QHPKLLRMVQDDLFHHLIHYGASSSPLVLSMICSCILNIYHFLRKFMRLQLEAFFSFVLL 361

Query: 370 RLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLLCKLSFPW 427
           R+ +F     +QEVALEG+INFCRQ +FI+E YVNYDCDP+  N+FEE GK+LC+ +FP 
Sbjct: 362 RVTAFTGFLPLQEVALEGLINFCRQPAFIVEAYVNYDCDPMCRNIFEETGKVLCRHTFPT 417

BLAST of Sgr022361 vs. ExPASy Swiss-Prot
Match: Q42510 (ARF guanine-nucleotide exchange factor GNOM OS=Arabidopsis thaliana OX=3702 GN=GN PE=1 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 8.5e-94
Identity = 211/487 (43.33%), Postives = 293/487 (60.16%), Query Frame = 0

Query: 3   KRSDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDSSIQ 62
           K  +EE +            L+CM++TE+ AVLAV+RR +     +++S  DD  + S+ 
Sbjct: 11  KAIEEEPEDFECTDSSNTTTLACMIDTEIAAVLAVMRR-NVRWGGRYMSG-DDQLEHSLI 70

Query: 63  QSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSRSST 122
           QSLK+LR  +F   Q W TI P +Y+ PFLDVI+SD+  A  T +ALS++ KI+      
Sbjct: 71  QSLKALRKQVFSWNQPWHTISPMLYLQPFLDVIRSDETGAPITSIALSSVYKILNLNVID 130

Query: 123 RNSWS-QRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSVCT 182
           +N+ + +    L+V  +T+C+ E TD  +E+ V+MKILQVL   M ++AS +L++Q VCT
Sbjct: 131 QNTANIEDAMHLVVDSVTSCRFEVTDPASEEVVLMKILQVLLACMKNKASVMLSNQHVCT 190

Query: 183 IVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIE------------------ 242
           +VNTCF VV Q+  +G+LLQR AR+TMHEL++ IFS LP++E                  
Sbjct: 191 VVNTCFRVVHQAGMKGELLQRVARHTMHELVRCIFSHLPDVERTETTLVNRAGSIKQEKA 250

Query: 243 ------------VRDGE-DSESDTE---------------DADLG-GS-----------L 302
                       V DG  +SE D E               D  +G GS           +
Sbjct: 251 GVDSDYAIVSKPVEDGNANSEYDVENSMATFATGAQSLMDDGPVGPGSRKPASPYDLHIM 310

Query: 303 DSGYGIRCVIDVFHFLCSLLNVVEMVEVDGSTSRTA--DEDVQLFALVLINSAVELSGDA 362
              YG+  ++++FHFLCSLLNVVE V + GS S T   DEDV LFAL LINSA+EL G +
Sbjct: 311 TEPYGVPSMVEIFHFLCSLLNVVEHVGM-GSRSNTIAFDEDVPLFALNLINSAIELGGSS 370

Query: 363 IGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFFVYV 422
           I  HP+LL ++QD+LF +L+ +G   +PL+LSM+CS VLN+Y  LR  ++LQLEAFF  V
Sbjct: 371 IRHHPRLLSLIQDELFRNLMQFGLSMSPLILSMVCSIVLNLYQHLRTELKLQLEAFFSCV 430

Query: 423 ALRLA--SFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLLCKL 427
            LRLA   +G S Q QEVA+E ++NFCRQ SF++E+Y N DCD    N+FEE+  LL K 
Sbjct: 431 ILRLAQGKYGPSYQQQEVAMEALVNFCRQKSFMVEMYANLDCDITCSNVFEELSNLLSKS 490

BLAST of Sgr022361 vs. ExPASy Swiss-Prot
Match: Q9FLY5 (ARF guanine-nucleotide exchange factor GNL1 OS=Arabidopsis thaliana OX=3702 GN=GNL1 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 5.9e-87
Identity = 193/469 (41.15%), Postives = 278/469 (59.28%), Query Frame = 0

Query: 23  LSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDSSIQQSLKSLRALIFHPQQKWRTI 82
           ++ M+N+E+GAVLAV+RR +     ++I+  DD  + S+  SLK LR  IF  Q  W+ +
Sbjct: 29  VASMINSEIGAVLAVMRR-NVRWGVRYIAD-DDQLEHSLIHSLKELRKQIFSWQSNWQYV 88

Query: 83  DPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSRSSTRNSWS-QRRNQLIVLGITNC 142
           DP +YI PFLDVI SD+  A  TGVALS++ KI+     T  + +      +IV  + +C
Sbjct: 89  DPRLYIQPFLDVILSDETGAPITGVALSSVYKILTLEVFTLETVNVGEAMHIIVDAVKSC 148

Query: 143 KLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLLQ 202
           + E TD  +E+ V+MKILQVL   +  +AS  L++Q +CTIVNTC  VV QS+S+ +LLQ
Sbjct: 149 RFEVTDPASEEVVLMKILQVLLACVKSKASNGLSNQDICTIVNTCLRVVHQSSSKSELLQ 208

Query: 203 RTARYTMHELIQIIFSRL------------------------------------------ 262
           R AR+TMHELI+ IFS+L                                          
Sbjct: 209 RIARHTMHELIRCIFSQLPFISPLANECELHVDNKVGTVDWDPNSGEKRVENGNIASISD 268

Query: 263 ---------------PEIEVRDGEDSESDTEDADLGGSLDSG----YGIRCVIDVFHFLC 322
                          PE ++R+ E     ++D +   + ++     YGI C++++FHFLC
Sbjct: 269 TLGTDKDDPSSEMVIPETDLRNDEKKTEVSDDLNAAANGENAMMAPYGIPCMVEIFHFLC 328

Query: 323 SLLNVVEMVEVDGSTSRTA-DEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHH 382
           +LLNV E  EV+  ++  A DEDV LFAL LINSA+EL G +  +HPKLL ++QDDLF +
Sbjct: 329 TLLNVGENGEVNSRSNPIAFDEDVPLFALGLINSAIELGGPSFREHPKLLTLIQDDLFCN 388

Query: 383 LIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFFVYVALRLA--SFGNSTQIQEV 427
           L+ +G   +PL+LS +CS VLN+Y  LR  +++QLEAFF YV LR+A    G+S Q QEV
Sbjct: 389 LMQFGMSMSPLILSTVCSIVLNLYLNLRTELKVQLEAFFSYVLLRIAQSKHGSSYQQQEV 448

BLAST of Sgr022361 vs. ExPASy Swiss-Prot
Match: Q9P7R8 (Uncharacterized protein C211.03 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC211.03c PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.4e-18
Identity = 98/399 (24.56%), Postives = 173/399 (43.36%), Query Frame = 0

Query: 88  ISPFLDVIQSDDIPAAATGVALSALLKI----IRSRSSTRNSWSQRRNQLIVLGITNCKL 147
           +SPFL  I+S  +    T + LSA+LK     I S  S   + S R    +   IT C+ 
Sbjct: 106 LSPFLRTIKSPRMTGYITSLCLSAILKFFSFRIISEESPNLALSMRN---LSFAITQCRF 165

Query: 148 EKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLLQRT 207
           E  D   ++AV++++ +++ EL+      +L+D S+C IV T  ++  QS     +L+ +
Sbjct: 166 ESFDASQDEAVLLRVSRLMEELLRGPGKAVLSDDSICEIVETGLSMCCQS-RLSQVLRYS 225

Query: 208 ARYTMHELIQIIFSRLPEIEVR-DGED----SESD------------TEDADLGGSLDSG 267
           A  +M  +++ IF RL  I+V+ D ED    SE              TE  ++   +D  
Sbjct: 226 AELSMTSILEKIFERLKYIDVKTDSEDFWDASEEHSIKGEEFHYKKITEGDEISNEIDP- 285

Query: 268 YGIRCVIDVFHFLCSLLNVVEMVEVDGSTSRTADEDVQLFALVLINSAVELSGDAIGKHP 327
           +GI  + +VF  L S         +D    + +D +++  AL  IN+A+E+SG  I   P
Sbjct: 286 FGIGSIREVFRVLVSF--------IDFGKQKFSD-NIKAMALRFINTALEVSGSHISDFP 345

Query: 328 KLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFFVYVALRL- 387
            L  ++ D L   L+      +  +L+     +  +   +   ++LQ E F  Y+   L 
Sbjct: 346 SLRVLITDTLCKSLLQLIRSDHTALLTNSLLVMTTLLQAMPGSLKLQQELFISYLISCLH 405

Query: 388 -----------------------------------ASF-----------GNSTQIQEVAL 419
                                               SF               +++E+ +
Sbjct: 406 IPSTTPRERNVETSLHKVVSSLDLSEEISPDRATPTSFTERKRFAFDTRNRPPEVRELIV 465

BLAST of Sgr022361 vs. ExPASy Swiss-Prot
Match: G5EGS5 (Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 homolog OS=Caenorhabditis elegans OX=6239 GN=gbf-1 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.1e-15
Identity = 106/530 (20.00%), Postives = 204/530 (38.49%), Query Frame = 0

Query: 30  EVGAVLAVIRRPSSELNSQHISPIDDTFDSSIQQSLKSLRALIFHPQQKWRTIDPSIYIS 89
           E   V+A++ +   +     +  ++DT    + ++   L+  + +       ++P  Y+S
Sbjct: 12  EANCVVALLNKARRQYQLSQVPTLEDT--DPLLRNFTDLKE-VLNEVADLADMNPQTYLS 71

Query: 90  PFLDVIQSDDIPAAATGVALSALLKIIRSRSSTRNS-WSQRRNQLIVLGITNCK-LEKTD 149
           PFLDVI++ +     T  AL+A+ K +       +S  +    + I   + + K +    
Sbjct: 72  PFLDVIKAQNTNGPITEAALAAVAKFLNYGLIDASSIKAANAVESIAYAVVHTKFIGGKS 131

Query: 150 LVTEDAVMMKILQVLAELMNHRASYLLNDQSVCTIVNTCFNVV-QQSASRGDLLQRTARY 209
             +++ V+ KILQVL  L+      LL++++VC ++ +CF +V +Q+ S   LL++ A  
Sbjct: 132 TGSDECVLFKILQVLRSLLLSPPGILLSNEAVCDMMQSCFRIVFEQNLSL--LLRKAAES 191

Query: 210 TMHELIQIIFSRLP---------------------------------------------- 269
           T+ ++ Q+IF+RLP                                              
Sbjct: 192 TLADMTQLIFTRLPTFVEDTRHPYIRQLVNPTEKRQKRKKKRQLSVHIETKAKEPENVPT 251

Query: 270 ---------------------------------------------------EIEVRDGED 329
                                                               I   D  D
Sbjct: 252 EMTKLIGEAAETAETDGAANLGYDVVLTTDPPVDTVTHPDPPIEEIIKLAEPISAGDEAD 311

Query: 330 SE--------------------------SDTEDAD----LGGSLDSGYGIRCVIDVFHFL 389
           SE                          SD E+ D    +GG     YG+ C  ++  FL
Sbjct: 312 SESEGGGGEEHHERPPVRAHAGLQREIVSDEEEIDTEQTVGGEEKMPYGLPCCRELLRFL 371

Query: 390 CSLLNVVEMVEVDGSTSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHH 423
            ++ N V+         R   E + +  L L+  A+E   D +  +  L+ +++++L  +
Sbjct: 372 ITMTNPVD---------RHNTESMVILGLNLLIVALEAIADFLPNYDILMPLIKNELCRN 431

BLAST of Sgr022361 vs. ExPASy TrEMBL
Match: A0A6J1DTF7 (ARF guanine-nucleotide exchange factor GNL2 OS=Momordica charantia OX=3673 GN=LOC111024228 PE=4 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 2.8e-209
Identity = 382/428 (89.25%), Postives = 402/428 (93.93%), Query Frame = 0

Query: 1   MEKRSDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDSS 60
           M KRSDEEDD++SSK+KRRELGLSCMLNTEVGA+LAVIRRP SELNS +ISPIDDTFDSS
Sbjct: 1   MTKRSDEEDDRDSSKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYISPIDDTFDSS 60

Query: 61  IQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR- 120
           IQQSLKSLRALIFHPQQKWRTIDPSIYISP LDV+QSDDIPAAATGVALSALLKII+   
Sbjct: 61  IQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVVQSDDIPAAATGVALSALLKIIKVEI 120

Query: 121 SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSV 180
            + +   ++    LIVLGITNCKLEKTDL+TEDAVMMKILQVLA LMNHRASYLLNDQSV
Sbjct: 121 FNEKTPGAKDAINLIVLGITNCKLEKTDLITEDAVMMKILQVLAGLMNHRASYLLNDQSV 180

Query: 181 CTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDADL 240
           CTIVNTCFNVVQQSA+RGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDADL
Sbjct: 181 CTIVNTCFNVVQQSANRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDADL 240

Query: 241 GGSLDSGYGIRCVIDVFHFLCSLLNVVEMVE-VDGSTSRTADEDVQLFALVLINSAVELS 300
           GGSLDSGYGIRCVIDVFHFLCSL+NVVEM E +DG  SRTADEDVQLFALVLINSAVELS
Sbjct: 241 GGSLDSGYGIRCVIDVFHFLCSLMNVVEMGEMMDGLASRTADEDVQLFALVLINSAVELS 300

Query: 301 GDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFF 360
           GDAIGKHP+LLRMVQDDLFHHLIHYGA  NPLVLSMICSTVLN+YHFLRRFVRLQLEAFF
Sbjct: 301 GDAIGKHPRLLRMVQDDLFHHLIHYGARCNPLVLSMICSTVLNIYHFLRRFVRLQLEAFF 360

Query: 361 VYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLLCK 420
           VYVALR AS+GNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNLFE+IGKLLCK
Sbjct: 361 VYVALRSASYGNSTQIQEVALEGIINFCRQSSFILETYVNYDCDPLRWNLFEDIGKLLCK 420

Query: 421 LSFPWGAP 427
           LSFP G+P
Sbjct: 421 LSFPAGSP 428

BLAST of Sgr022361 vs. ExPASy TrEMBL
Match: A0A5A7V061 (ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00190 PE=4 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 5.5e-205
Identity = 378/430 (87.91%), Postives = 400/430 (93.02%), Query Frame = 0

Query: 1   MEKR-SDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDS 60
           M+KR SD+ED+K+S K+KRRELGLSCMLNTEVGA+LAVIRRP SELNS +I+ ID+T+DS
Sbjct: 1   MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 60

Query: 61  SIQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR 120
           +IQQSLKSLRALIFHPQQKWRTIDPSIYISP LDVIQSDDIPAAATGVALSALLKII+  
Sbjct: 61  TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121 -SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQS 180
               +    +    LIVLGITNCKLEKTDLVTEDAVMMK+LQVLA LMNHRASYLLNDQS
Sbjct: 121 IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDAD 240
           VCTIVNTCFNVVQQSASRGDLLQRTARYTM+ELIQIIFSRLPE+EVRDGE+SESDTEDAD
Sbjct: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 240

Query: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMVEV--DGSTSRTADEDVQLFALVLINSAVE 300
           LGGS+DSGYGIRCVIDVFHFLCSLLNVVEMVEV   G  SRTADEDVQLFALVLINSAVE
Sbjct: 241 LGGSMDSGYGIRCVIDVFHFLCSLLNVVEMVEVGDGGLGSRTADEDVQLFALVLINSAVE 300

Query: 301 LSGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEA 360
           LSGDAIGKHPKLLRMVQDDLFHHLIHYGA SNPLVLSMICSTVLN+YHFLRRFVRLQLEA
Sbjct: 301 LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360

Query: 361 FFVYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLL 420
           F +YVALRLASFGNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNLFEEIGKLL
Sbjct: 361 FSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420

Query: 421 CKLSFPWGAP 427
           CKLSFP G+P
Sbjct: 421 CKLSFPTGSP 430

BLAST of Sgr022361 vs. ExPASy TrEMBL
Match: A0A1S3C8R1 (ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo OX=3656 GN=LOC103497742 PE=4 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.2e-204
Identity = 377/430 (87.67%), Postives = 400/430 (93.02%), Query Frame = 0

Query: 1   MEKR-SDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDS 60
           M+KR SD+ED+K+S K+KRRELGLSCMLNTEVGA+LAVIRRP SELNS +I+ ID+T+DS
Sbjct: 3   MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 62

Query: 61  SIQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR 120
           +IQQSLKSLRALIFHPQQKWRTIDPSIYISP LDVIQSDDIPAAATGVALSALLKII+  
Sbjct: 63  TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 122

Query: 121 -SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQS 180
               +    +    LIVLGITNCKLEKTDLVTEDAVMMK+LQVLA LMNHRASYLLNDQS
Sbjct: 123 IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 182

Query: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDAD 240
           VCTIVNTCFNVVQQSASRGDLLQRTARYTM+ELIQIIFSRLPE+EVRDGE+SESDTEDAD
Sbjct: 183 VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 242

Query: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMVEV--DGSTSRTADEDVQLFALVLINSAVE 300
           LGGS+DSGYGIRCVIDVFHFLCSLLNVVEMVEV   G  SRTADEDVQLFALVLINSAVE
Sbjct: 243 LGGSMDSGYGIRCVIDVFHFLCSLLNVVEMVEVGDGGLGSRTADEDVQLFALVLINSAVE 302

Query: 301 LSGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEA 360
           LSGDAIGKHPKLLRMVQDDLFHHLIHYGA SNPLVLSMICSTVLN+YHFLRRFVRLQLEA
Sbjct: 303 LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 362

Query: 361 FFVYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLL 420
           F +YVALRLASFGNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNLFEE+GKLL
Sbjct: 363 FSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEMGKLL 422

Query: 421 CKLSFPWGAP 427
           CKLSFP G+P
Sbjct: 423 CKLSFPTGSP 432

BLAST of Sgr022361 vs. ExPASy TrEMBL
Match: A0A0A0LF22 (SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G004740 PE=4 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 6.1e-204
Identity = 378/431 (87.70%), Postives = 401/431 (93.04%), Query Frame = 0

Query: 1   MEKR-SDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDS 60
           M+KR SD+ED+K+S K+KRRELGLSCMLNTEVG++LAVIRRP SELNS +IS ID+T+DS
Sbjct: 1   MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60

Query: 61  SIQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR 120
           +IQQSLKSLRALIFHPQQKWRTIDPSIYISP LDVIQSDDIPAAATGVALSALLKII+  
Sbjct: 61  TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121 -SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQS 180
               +   ++    LIV+GITNCKLEKTDLVTEDAVMMK+LQVLA LMNHRASYLLNDQS
Sbjct: 121 IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDAD 240
           VCTIVNTCFNVVQQSASRGDLLQRTARYTM+ELIQIIFSRLPEIEVRDGE+SESDTEDAD
Sbjct: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240

Query: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVE-MVEV--DGSTSRTADEDVQLFALVLINSAV 300
           LGGSLDSGYGIRCVIDVFHFLCSLLNVVE MVEV   G  SRTADEDVQLFALVLINSAV
Sbjct: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300

Query: 301 ELSGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLE 360
           ELSGDAIGKHPKLLRMVQDDLFHHLIHYGA SNPLVLSMICSTVLN+YHFLRRFVRLQLE
Sbjct: 301 ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360

Query: 361 AFFVYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKL 420
           +FFVYVAL+LASFGNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNL EEIGKL
Sbjct: 361 SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420

Query: 421 LCKLSFPWGAP 427
           LCKLSFP G+P
Sbjct: 421 LCKLSFPTGSP 431

BLAST of Sgr022361 vs. ExPASy TrEMBL
Match: A0A6J1FBD6 (ARF guanine-nucleotide exchange factor GNL2 OS=Cucurbita moschata OX=3662 GN=LOC111443895 PE=4 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 3.4e-202
Identity = 376/429 (87.65%), Postives = 392/429 (91.38%), Query Frame = 0

Query: 1   MEKRSDEEDD-KESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDS 60
           MEKRS   DD KESSK+KRRELGLSCMLNTEVGAVLAVIRRP SELN+ +I   DDT+DS
Sbjct: 1   MEKRSSRHDDGKESSKSKRRELGLSCMLNTEVGAVLAVIRRPPSELNAPYICTADDTYDS 60

Query: 61  SIQQSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR 120
           SIQQSLKSLRALIF PQQKWRTIDPSIYISP LDVIQSDDIPAAATGVALSALLKII+  
Sbjct: 61  SIQQSLKSLRALIFQPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121 -SSTRNSWSQRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQS 180
               +   ++    LIVLGITNCKLEKTD+VTEDAVMMKILQVLA LMNHRASYLLNDQS
Sbjct: 121 IFEEKTPGAKDAINLIVLGITNCKLEKTDVVTEDAVMMKILQVLAGLMNHRASYLLNDQS 180

Query: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDAD 240
           VCTIVNTCFNVVQQSASRGDLLQRTARYTM+ELIQIIFSRLPE+E RD EDSESDTEDAD
Sbjct: 181 VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEFRDEEDSESDTEDAD 240

Query: 241 LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMVE-VDGSTSRTADEDVQLFALVLINSAVEL 300
           LGGS+DSGYGIRCVIDVFHFLCSLLNVVE  E VDG  SRTADEDVQLFALVLINSAV L
Sbjct: 241 LGGSMDSGYGIRCVIDVFHFLCSLLNVVETAEMVDGLGSRTADEDVQLFALVLINSAVGL 300

Query: 301 SGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAF 360
           SGDAIGKHPKLLRM+QDDLFHHLIHYGA SNPLVLSMICSTVLN+YHFLRRFVRLQLEAF
Sbjct: 301 SGDAIGKHPKLLRMIQDDLFHHLIHYGARSNPLVLSMICSTVLNIYHFLRRFVRLQLEAF 360

Query: 361 FVYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLLC 420
           F+YV LRLASFGNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNLFEEIGKLLC
Sbjct: 361 FIYVVLRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLLC 420

Query: 421 KLSFPWGAP 427
           KLSFP G+P
Sbjct: 421 KLSFPMGSP 429

BLAST of Sgr022361 vs. TAIR 10
Match: AT5G19610.1 (GNOM-like 2 )

HSP 1 Score: 559.3 bits (1440), Expect = 2.7e-159
Identity = 290/423 (68.56%), Postives = 348/423 (82.27%), Query Frame = 0

Query: 10  DKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPID-DTFDSSIQQSLKSL 69
           D+ + + KR+ELG+SCMLNTEVGAVLAVIRRP SE    ++SP + D  DSS+QQSLKSL
Sbjct: 2   DRIAVRAKRKELGISCMLNTEVGAVLAVIRRPLSE---SYLSPQETDHCDSSVQQSLKSL 61

Query: 70  RALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSR-SSTRNSWS 129
           RALIF+PQQ WRTIDPS+Y+SPFL+VIQSD+IPA+AT VALS++LKI++      +   +
Sbjct: 62  RALIFNPQQDWRTIDPSVYLSPFLEVIQSDEIPASATAVALSSILKILKIEIFDEKTPGA 121

Query: 130 QRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSVCTIVNTCF 189
           +     IV GIT+C+LEKTDLV+EDAVMM+ILQVL  +M H +S LL DQ+VCTIVNTCF
Sbjct: 122 KDAMNSIVSGITSCRLEKTDLVSEDAVMMRILQVLTGIMKHPSSELLEDQAVCTIVNTCF 181

Query: 190 NVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIEVR---DGEDSESDTEDADLGGSLD 249
            VVQQS  RGDLLQR  RYTMHELIQIIFSRLP+ EVR    GEDSESDT++ D+ G   
Sbjct: 182 QVVQQSTGRGDLLQRNGRYTMHELIQIIFSRLPDFEVRGDEGGEDSESDTDEIDMSG--- 241

Query: 250 SGYGIRCVIDVFHFLCSLLNVVEMVE-VDGSTSRTADEDVQLFALVLINSAVELSGDAIG 309
            GYGIRC ID+FHFLCSLLNVVE+VE ++G+   TADEDVQ+FALVLINSA+ELSGDAIG
Sbjct: 242 -GYGIRCCIDIFHFLCSLLNVVEVVENLEGTNVHTADEDVQIFALVLINSAIELSGDAIG 301

Query: 310 KHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFFVYVAL 369
           +HPKLLRMVQDDLFHHLIHYGA S+PLVLSMICS +LN+YHFLR+F+RLQLEAFF +V L
Sbjct: 302 QHPKLLRMVQDDLFHHLIHYGASSSPLVLSMICSCILNIYHFLRKFMRLQLEAFFSFVLL 361

Query: 370 RLASFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLLCKLSFPW 427
           R+ +F     +QEVALEG+INFCRQ +FI+E YVNYDCDP+  N+FEE GK+LC+ +FP 
Sbjct: 362 RVTAFTGFLPLQEVALEGLINFCRQPAFIVEAYVNYDCDPMCRNIFEETGKVLCRHTFPT 417

BLAST of Sgr022361 vs. TAIR 10
Match: AT1G13980.1 (sec7 domain-containing protein )

HSP 1 Score: 345.5 bits (885), Expect = 6.0e-95
Identity = 211/487 (43.33%), Postives = 293/487 (60.16%), Query Frame = 0

Query: 3   KRSDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDSSIQ 62
           K  +EE +            L+CM++TE+ AVLAV+RR +     +++S  DD  + S+ 
Sbjct: 11  KAIEEEPEDFECTDSSNTTTLACMIDTEIAAVLAVMRR-NVRWGGRYMSG-DDQLEHSLI 70

Query: 63  QSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSRSST 122
           QSLK+LR  +F   Q W TI P +Y+ PFLDVI+SD+  A  T +ALS++ KI+      
Sbjct: 71  QSLKALRKQVFSWNQPWHTISPMLYLQPFLDVIRSDETGAPITSIALSSVYKILNLNVID 130

Query: 123 RNSWS-QRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSVCT 182
           +N+ + +    L+V  +T+C+ E TD  +E+ V+MKILQVL   M ++AS +L++Q VCT
Sbjct: 131 QNTANIEDAMHLVVDSVTSCRFEVTDPASEEVVLMKILQVLLACMKNKASVMLSNQHVCT 190

Query: 183 IVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIE------------------ 242
           +VNTCF VV Q+  +G+LLQR AR+TMHEL++ IFS LP++E                  
Sbjct: 191 VVNTCFRVVHQAGMKGELLQRVARHTMHELVRCIFSHLPDVERTETTLVNRAGSIKQEKA 250

Query: 243 ------------VRDGE-DSESDTE---------------DADLG-GS-----------L 302
                       V DG  +SE D E               D  +G GS           +
Sbjct: 251 GVDSDYAIVSKPVEDGNANSEYDVENSMATFATGAQSLMDDGPVGPGSRKPASPYDLHIM 310

Query: 303 DSGYGIRCVIDVFHFLCSLLNVVEMVEVDGSTSRTA--DEDVQLFALVLINSAVELSGDA 362
              YG+  ++++FHFLCSLLNVVE V + GS S T   DEDV LFAL LINSA+EL G +
Sbjct: 311 TEPYGVPSMVEIFHFLCSLLNVVEHVGM-GSRSNTIAFDEDVPLFALNLINSAIELGGSS 370

Query: 363 IGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFFVYV 422
           I  HP+LL ++QD+LF +L+ +G   +PL+LSM+CS VLN+Y  LR  ++LQLEAFF  V
Sbjct: 371 IRHHPRLLSLIQDELFRNLMQFGLSMSPLILSMVCSIVLNLYQHLRTELKLQLEAFFSCV 430

Query: 423 ALRLA--SFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLLCKL 427
            LRLA   +G S Q QEVA+E ++NFCRQ SF++E+Y N DCD    N+FEE+  LL K 
Sbjct: 431 ILRLAQGKYGPSYQQQEVAMEALVNFCRQKSFMVEMYANLDCDITCSNVFEELSNLLSKS 490

BLAST of Sgr022361 vs. TAIR 10
Match: AT1G13980.2 (sec7 domain-containing protein )

HSP 1 Score: 345.5 bits (885), Expect = 6.0e-95
Identity = 211/487 (43.33%), Postives = 293/487 (60.16%), Query Frame = 0

Query: 3   KRSDEEDDKESSKTKRRELGLSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDSSIQ 62
           K  +EE +            L+CM++TE+ AVLAV+RR +     +++S  DD  + S+ 
Sbjct: 11  KAIEEEPEDFECTDSSNTTTLACMIDTEIAAVLAVMRR-NVRWGGRYMSG-DDQLEHSLI 70

Query: 63  QSLKSLRALIFHPQQKWRTIDPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSRSST 122
           QSLK+LR  +F   Q W TI P +Y+ PFLDVI+SD+  A  T +ALS++ KI+      
Sbjct: 71  QSLKALRKQVFSWNQPWHTISPMLYLQPFLDVIRSDETGAPITSIALSSVYKILNLNVID 130

Query: 123 RNSWS-QRRNQLIVLGITNCKLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSVCT 182
           +N+ + +    L+V  +T+C+ E TD  +E+ V+MKILQVL   M ++AS +L++Q VCT
Sbjct: 131 QNTANIEDAMHLVVDSVTSCRFEVTDPASEEVVLMKILQVLLACMKNKASVMLSNQHVCT 190

Query: 183 IVNTCFNVVQQSASRGDLLQRTARYTMHELIQIIFSRLPEIE------------------ 242
           +VNTCF VV Q+  +G+LLQR AR+TMHEL++ IFS LP++E                  
Sbjct: 191 VVNTCFRVVHQAGMKGELLQRVARHTMHELVRCIFSHLPDVERTETTLVNRAGSIKQEKA 250

Query: 243 ------------VRDGE-DSESDTE---------------DADLG-GS-----------L 302
                       V DG  +SE D E               D  +G GS           +
Sbjct: 251 GVDSDYAIVSKPVEDGNANSEYDVENSMATFATGAQSLMDDGPVGPGSRKPASPYDLHIM 310

Query: 303 DSGYGIRCVIDVFHFLCSLLNVVEMVEVDGSTSRTA--DEDVQLFALVLINSAVELSGDA 362
              YG+  ++++FHFLCSLLNVVE V + GS S T   DEDV LFAL LINSA+EL G +
Sbjct: 311 TEPYGVPSMVEIFHFLCSLLNVVEHVGM-GSRSNTIAFDEDVPLFALNLINSAIELGGSS 370

Query: 363 IGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFFVYV 422
           I  HP+LL ++QD+LF +L+ +G   +PL+LSM+CS VLN+Y  LR  ++LQLEAFF  V
Sbjct: 371 IRHHPRLLSLIQDELFRNLMQFGLSMSPLILSMVCSIVLNLYQHLRTELKLQLEAFFSCV 430

Query: 423 ALRLA--SFGNSTQIQEVALEGIINFCRQSSFILEIYVNYDCDPLRWNLFEEIGKLLCKL 427
            LRLA   +G S Q QEVA+E ++NFCRQ SF++E+Y N DCD    N+FEE+  LL K 
Sbjct: 431 ILRLAQGKYGPSYQQQEVAMEALVNFCRQKSFMVEMYANLDCDITCSNVFEELSNLLSKS 490

BLAST of Sgr022361 vs. TAIR 10
Match: AT5G39500.1 (GNOM-like 1 )

HSP 1 Score: 322.8 bits (826), Expect = 4.2e-88
Identity = 193/469 (41.15%), Postives = 278/469 (59.28%), Query Frame = 0

Query: 23  LSCMLNTEVGAVLAVIRRPSSELNSQHISPIDDTFDSSIQQSLKSLRALIFHPQQKWRTI 82
           ++ M+N+E+GAVLAV+RR +     ++I+  DD  + S+  SLK LR  IF  Q  W+ +
Sbjct: 29  VASMINSEIGAVLAVMRR-NVRWGVRYIAD-DDQLEHSLIHSLKELRKQIFSWQSNWQYV 88

Query: 83  DPSIYISPFLDVIQSDDIPAAATGVALSALLKIIRSRSSTRNSWS-QRRNQLIVLGITNC 142
           DP +YI PFLDVI SD+  A  TGVALS++ KI+     T  + +      +IV  + +C
Sbjct: 89  DPRLYIQPFLDVILSDETGAPITGVALSSVYKILTLEVFTLETVNVGEAMHIIVDAVKSC 148

Query: 143 KLEKTDLVTEDAVMMKILQVLAELMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLLQ 202
           + E TD  +E+ V+MKILQVL   +  +AS  L++Q +CTIVNTC  VV QS+S+ +LLQ
Sbjct: 149 RFEVTDPASEEVVLMKILQVLLACVKSKASNGLSNQDICTIVNTCLRVVHQSSSKSELLQ 208

Query: 203 RTARYTMHELIQIIFSRL------------------------------------------ 262
           R AR+TMHELI+ IFS+L                                          
Sbjct: 209 RIARHTMHELIRCIFSQLPFISPLANECELHVDNKVGTVDWDPNSGEKRVENGNIASISD 268

Query: 263 ---------------PEIEVRDGEDSESDTEDADLGGSLDSG----YGIRCVIDVFHFLC 322
                          PE ++R+ E     ++D +   + ++     YGI C++++FHFLC
Sbjct: 269 TLGTDKDDPSSEMVIPETDLRNDEKKTEVSDDLNAAANGENAMMAPYGIPCMVEIFHFLC 328

Query: 323 SLLNVVEMVEVDGSTSRTA-DEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHH 382
           +LLNV E  EV+  ++  A DEDV LFAL LINSA+EL G +  +HPKLL ++QDDLF +
Sbjct: 329 TLLNVGENGEVNSRSNPIAFDEDVPLFALGLINSAIELGGPSFREHPKLLTLIQDDLFCN 388

Query: 383 LIHYGACSNPLVLSMICSTVLNVYHFLRRFVRLQLEAFFVYVALRLA--SFGNSTQIQEV 427
           L+ +G   +PL+LS +CS VLN+Y  LR  +++QLEAFF YV LR+A    G+S Q QEV
Sbjct: 389 LMQFGMSMSPLILSTVCSIVLNLYLNLRTELKVQLEAFFSYVLLRIAQSKHGSSYQQQEV 448

BLAST of Sgr022361 vs. TAIR 10
Match: AT1G01960.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 57.8 bits (138), Expect = 2.5e-08
Identity = 49/203 (24.14%), Postives = 84/203 (41.38%), Query Frame = 0

Query: 223 EVRDGEDSESDTEDADLGGSLDSGYGIRCVIDVFHFLCSLLNVVEMVEVDGSTSRTADED 282
           E+ DGE  + D  +  +G  L      R    VF  LC L           S      ED
Sbjct: 316 ELADGEVEKDDDSEVQIGNKLR-----RDAFLVFRALCKL-----------SMKTPPKED 375

Query: 283 VQLF-----ALVLINSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGACSNPLVLSMICS 342
            +L      AL L+   +E +G       + L  ++  L   L+   A +  ++  + CS
Sbjct: 376 PELMRGKIVALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSASNLMIIFQLSCS 435

Query: 343 TVLNVYHFLRRFVRLQLEAFFVYVALRLASFGNSTQIQE--VALEGIINFCRQSSFILEI 402
            +L++    R  ++ ++  FF  + LR+         Q+  + L  +   C  S  +++I
Sbjct: 436 ILLSLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPDFQQKMIVLRFLDKLCVDSQILVDI 495

Query: 403 YVNYDCDPLRWNLFEEIGKLLCK 419
           ++NYDCD    N+FE +   L K
Sbjct: 496 FINYDCDVNSSNIFERMVNGLLK 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022157565.15.9e-20989.25ARF guanine-nucleotide exchange factor GNL2 [Momordica charantia][more]
XP_038889761.11.6e-20688.48ARF guanine-nucleotide exchange factor GNL2 [Benincasa hispida][more]
KAA0061562.11.1e-20487.91ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo var. makuwa] >TYK10710... [more]
XP_008458277.12.6e-20487.67PREDICTED: ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo][more]
XP_004139429.11.3e-20387.70ARF guanine-nucleotide exchange factor GNL2 [Cucumis sativus] >KGN60625.1 hypoth... [more]
Match NameE-valueIdentityDescription
F4K2K33.7e-15868.56ARF guanine-nucleotide exchange factor GNL2 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q425108.5e-9443.33ARF guanine-nucleotide exchange factor GNOM OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q9FLY55.9e-8741.15ARF guanine-nucleotide exchange factor GNL1 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q9P7R83.4e-1824.56Uncharacterized protein C211.03 OS=Schizosaccharomyces pombe (strain 972 / ATCC ... [more]
G5EGS52.1e-1520.00Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 homol... [more]
Match NameE-valueIdentityDescription
A0A6J1DTF72.8e-20989.25ARF guanine-nucleotide exchange factor GNL2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A5A7V0615.5e-20587.91ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C8R11.2e-20487.67ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo OX=3656 GN=LOC103497... [more]
A0A0A0LF226.1e-20487.70SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G004740 PE=4 S... [more]
A0A6J1FBD63.4e-20287.65ARF guanine-nucleotide exchange factor GNL2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G19610.12.7e-15968.56GNOM-like 2 [more]
AT1G13980.16.0e-9543.33sec7 domain-containing protein [more]
AT1G13980.26.0e-9543.33sec7 domain-containing protein [more]
AT5G39500.14.2e-8841.15GNOM-like 1 [more]
AT1G01960.12.5e-0824.14SEC7-like guanine nucleotide exchange family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032691Guanine nucleotide exchange factor, N-terminalPFAMPF12783Sec7_Ncoord: 249..405
e-value: 1.4E-27
score: 96.5
IPR032629Mon2, dimerisation and cyclophilin-binding domainPFAMPF16213DCBcoord: 85..219
e-value: 9.9E-8
score: 31.9
NoneNo IPR availablePANTHERPTHR10663GUANYL-NUCLEOTIDE EXCHANGE FACTORcoord: 10..424
NoneNo IPR availablePANTHERPTHR10663:SF322ARF GUANINE-NUCLEOTIDE EXCHANGE FACTOR GNL2coord: 10..424
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 113..397

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr022361.1Sgr022361.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006298 mismatch repair
biological_process GO:0009846 pollen germination
biological_process GO:0015031 protein transport
biological_process GO:0032012 regulation of ARF protein signal transduction
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0005829 cytosol
cellular_component GO:0016020 membrane
cellular_component GO:0090406 pollen tube
molecular_function GO:0005524 ATP binding
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity
molecular_function GO:0030983 mismatched DNA binding