Sgr022320 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr022320
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionMitogen-activated protein kinase kinase
Locationtig00154107: 562390 .. 565054 (+)
RNA-Seq ExpressionSgr022320
SyntenySgr022320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGACCAAGACGCCCTTGAAGCAGCTCAAGCTCTCTGTTCCTGCTCAAGAAGCTACAATTCATTCTTTCCTGTTAGTTCAAATCATTTTTCTCTTATTTTTACTTAATTTTGAAGCTTCTTCGGTTTTCAGTGTCCTTCATGACGTTCATTTCGTTTCATTTCGTATTTCGTAATAGCTACTCTTTGTTTGATCCTGTGAAAATAATCTGTCCTGTGTTTTTCAATGGAGCTTCGATTGGTTTGTATTCGTACTTTTTTGGAAATTAACGCTGCGTAGAAGAAAAATATATTTGGAAGTTTGATGAACTTATCTGTAATTGACCGAATGAATTCAAATGACTGGCTTCATTGATGTGCTTTCCATTTCAAGCATTTCAAATTTCAGGAAGGATTAAGGGCATGATCTGTTCAATCTATTTTTTTACGTTTTAGCTATCGTTTTTCCCTATTTCCGTTTCCTGAGCTATAGTCACAAAAAAACAAATCTCCTCTATATGCGTTTTTTGATTTTGTTCAACTTCAGGACTGCAAGTGGAACATTTCACGACGGCAATCTGCTATTGAACCAGAAAGGGTTGCGGCTTATATCCGAAGAGAAAGAAGCGCAGGTAATTCGTTATTGTTCACAAACAGATGATGACATTATTTCAAACATCCTAATTTTAAGTTGCTGAAGTTAACCAATTTGCCATCTGAATTTGTGAAAACTCGCCTAACCTAGATGCTAACCGTGTGGTTTACATTCACAGTAGATGCATGATACTACCGTTCTTCCTGCCTAGGTTTCCTTGTCGCATCAAAATCACTTTTCCCTTTTTCATTTTGTTTGAACAGACGTCAGACTGTAAGGAGCTTGATGTCGATTTCTCATTGGAGGATCTAGAGACTGTGAAAGTGATCGGGAAAGGAAGTGGGGGTGTAGTACAACTTGTTCGCCACAAATGGGTTGGAAAACTTTTTGCCTTAAAGGTTGTTATATGGGTGGAACAATTCTACACTCTCGCATTTGTTTCTGGTCATTGTATCTTTGCTTTAAGTTATTCACACGCTTTGTTCCATTTTTTGTAGCTTTATGTCACTTGAAGAAGCTGCTGCTATAAGCTAGCAGCTTCTAATCATTACATTCAAATGTGGAGCTAGGTAGGTAGTAAACTGCTATTACAATCATTTAGCCATGAGAATAAATTCAAATTCCCTTTCCAAAATTAGTAGAGGTTGGTCTTGGTTCACAAGTTGATCTTGTAGAAGTCAAGGTTTCTTACAAGAATGTAGGGCGGTGAGGTAATCTCAACTCTTTGACCTAAAACGATACAATGAGCTTTGCTAAGGAAGTGGTAAAAGCGTGGCAATGCCTCAACTGGTGAGACCGTGAGAGAGTATTGTGGTAAACTATTTTTTCATATGATTATTTTAATAATGTGATAGGATTATGATTGATATTGATTGATGAAATCTATTTGTTACATGTCTTATTTTAGCTCAAACTACACTACCAAACTGTCTAAATACTGGCGCAAACAATTTGTTATCCTGTTTGTAAGTTTTTCAAATTCTGAAGCCATGGTAATCTTTTGCTTTCGGTTATCTTAGATCTATACGGTTTATTTATCCAAACTCCAAAGTACTAAAGATATCATTCTGCTTAGGTCTTATTGAATACTTATTTATGTTTGCTTTTGGTTACCATCATGTAATCTTCATTAAAAGCTACTATTACTAGTGCTATGCTGGCTTTTGCACATACTCAAATAATCTCGAGTCCAAGTCATATTAAGCGCTGATTTCCTGCCTTATGCTTCAGGTATGCAGATGAACATACAAGAGGATATCCGTAAACAAATAGTGCAGGAGCTGAAAATAAATCAAGCAGCACAATGTCCACATGTTGTGGTCTGCTATCATTCGTTCTACCACAATGGTGCCATTTCTCTTGTGCTAGAATACATGGATCGTGGTTCTTTAGCCGATGTTGTCAGACAAGTCAAAACAATTCTTGAACCATATCTTGCAGTTGTTTGTAAACAGGTCTCAGGATTCAGTCATTAAAATCAAAAGCTGAATGAAAGGTTAAGTTTTTACTCATGCTCACTTACTCCAATTATGTTCCCAGGTTTTACAAGGTCTTGTTTACTTGCATCATGAAAGACATGTTATCCACAGAGACATCAAACCATCCAATCTACTTGTGAACCATCAAGGGGAGGTCAAAATCACTGATTTTGGAGTAAGTGCAATGTTAGCAAGTTCTATTGGTCAGAGAGATACATTTGTAGGGACTTACAATTACATGTCGGTATGTGAAATTCCCCCGAGATTTATAACATTGAGCAGATTGTTTTTATTTGTTGATACAATTTTTTTAGTCATCTGCTTATGCACAGTGTTTATAACTGATATGAAGCATGACACTACTGGATTTTATTTCCCTTTCAGCCCGAGCGAATTAGCGGGGGAACATATGACTACAGCAGTGATATATGGAGTTTGGGCCTGGTAGTACTTGAGTGTGCCATTGGAAGGTTTCCTTATTTGCAGTCCGAAGAGCAGCAAAGTTGGCCAAGCTTTTATGAACTTTTAGAGGCAATTGTGGCGAAGCCATCGCCTTCTGCTCCACCGGATCAATTTTCCCCCGAGTTCTGTTCCTTCGTATCTGCTTGGTAA

mRNA sequence

ATGAAGACCAAGACGCCCTTGAAGCAGCTCAAGCTCTCTGTTCCTGCTCAAGAAGCTACAATTCATTCTTTCCTGACTGCAAGTGGAACATTTCACGACGGCAATCTGCTATTGAACCAGAAAGGGTTGCGGCTTATATCCGAAGAGAAAGAAGCGCAGACGTCAGACTGTAAGGAGCTTGATGTCGATTTCTCATTGGAGGATCTAGAGACTGTGAAAGTGATCGGGAAAGGAAGTGGGGGTGTAGTACAACTTGTTCGCCACAAATGGGTTGGAAAACTTTTTGCCTTAAAGGTTGTTATATGGGTGGAACAATTCTACACTCTCGCATTTGTTTCTGGTCATTGTATGCAGATGAACATACAAGAGGATATCCGTAAACAAATAGTGCAGGAGCTGAAAATAAATCAAGCAGCACAATGTCCACATGTTGTGGTCTGCTATCATTCGTTCTACCACAATGGTGCCATTTCTCTTGTGCTAGAATACATGGATCGTGGTTCTTTAGCCGATGTTGTCAGACAAGTCAAAACAATTCTTGAACCATATCTTGCAGTTGTTTGTAAACAGGTTTTACAAGGTCTTGTTTACTTGCATCATGAAAGACATGTTATCCACAGAGACATCAAACCATCCAATCTACTTGTGAACCATCAAGGGGAGGTCAAAATCACTGATTTTGGAGTAAGTGCAATGTTAGCAAGTTCTATTGGTCAGAGAGATACATTTGTAGGGACTTACAATTACATGTCGCCCGAGCGAATTAGCGGGGGAACATATGACTACAGCAGTGATATATGGAGTTTGGGCCTGGTAGTACTTGAGTGTGCCATTGGAAGGTTTCCTTATTTGCAGTCCGAAGAGCAGCAAAGTTGGCCAAGCTTTTATGAACTTTTAGAGGCAATTGTGGCGAAGCCATCGCCTTCTGCTCCACCGGATCAATTTTCCCCCGAGTTCTGTTCCTTCGTATCTGCTTGGTAA

Coding sequence (CDS)

ATGAAGACCAAGACGCCCTTGAAGCAGCTCAAGCTCTCTGTTCCTGCTCAAGAAGCTACAATTCATTCTTTCCTGACTGCAAGTGGAACATTTCACGACGGCAATCTGCTATTGAACCAGAAAGGGTTGCGGCTTATATCCGAAGAGAAAGAAGCGCAGACGTCAGACTGTAAGGAGCTTGATGTCGATTTCTCATTGGAGGATCTAGAGACTGTGAAAGTGATCGGGAAAGGAAGTGGGGGTGTAGTACAACTTGTTCGCCACAAATGGGTTGGAAAACTTTTTGCCTTAAAGGTTGTTATATGGGTGGAACAATTCTACACTCTCGCATTTGTTTCTGGTCATTGTATGCAGATGAACATACAAGAGGATATCCGTAAACAAATAGTGCAGGAGCTGAAAATAAATCAAGCAGCACAATGTCCACATGTTGTGGTCTGCTATCATTCGTTCTACCACAATGGTGCCATTTCTCTTGTGCTAGAATACATGGATCGTGGTTCTTTAGCCGATGTTGTCAGACAAGTCAAAACAATTCTTGAACCATATCTTGCAGTTGTTTGTAAACAGGTTTTACAAGGTCTTGTTTACTTGCATCATGAAAGACATGTTATCCACAGAGACATCAAACCATCCAATCTACTTGTGAACCATCAAGGGGAGGTCAAAATCACTGATTTTGGAGTAAGTGCAATGTTAGCAAGTTCTATTGGTCAGAGAGATACATTTGTAGGGACTTACAATTACATGTCGCCCGAGCGAATTAGCGGGGGAACATATGACTACAGCAGTGATATATGGAGTTTGGGCCTGGTAGTACTTGAGTGTGCCATTGGAAGGTTTCCTTATTTGCAGTCCGAAGAGCAGCAAAGTTGGCCAAGCTTTTATGAACTTTTAGAGGCAATTGTGGCGAAGCCATCGCCTTCTGCTCCACCGGATCAATTTTCCCCCGAGTTCTGTTCCTTCGTATCTGCTTGGTAA

Protein sequence

MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKELDVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMNIQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQRDTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPSPSAPPDQFSPEFCSFVSAW
Homology
BLAST of Sgr022320 vs. NCBI nr
Match: XP_022154636.1 (mitogen-activated protein kinase kinase 6 [Momordica charantia])

HSP 1 Score: 589.7 bits (1519), Expect = 1.5e-164
Identity = 300/325 (92.31%), Postives = 304/325 (93.54%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVPAQEA IHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSD KEL
Sbjct: 1   MKTKTPLKQLKLSVPAQEANIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDSKEL 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           D DFSLEDLETVKVIGKGSGGVVQLV+HKWVGKLFALKV+                 QMN
Sbjct: 61  DADFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVI-----------------QMN 120

Query: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180
           IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL
Sbjct: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180

Query: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQR 240
           EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNH+GEVKITDFGVSAMLASS+GQR
Sbjct: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAMLASSMGQR 240

Query: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300
           DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE
Sbjct: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300

Query: 301 AIVAKPSPSAPPDQFSPEFCSFVSA 326
           AIVAKP PSAPPDQFSPEFCSFVSA
Sbjct: 301 AIVAKPPPSAPPDQFSPEFCSFVSA 308

BLAST of Sgr022320 vs. NCBI nr
Match: XP_038890673.1 (mitogen-activated protein kinase kinase 6 [Benincasa hispida] >XP_038890674.1 mitogen-activated protein kinase kinase 6 [Benincasa hispida])

HSP 1 Score: 589.0 bits (1517), Expect = 2.6e-164
Identity = 297/325 (91.38%), Postives = 303/325 (93.23%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVP QE TI SFLTASGTFHDGNLLLNQKGLRLISEEKEAQT+DCKEL
Sbjct: 1   MKTKTPLKQLKLSVPVQETTIRSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTTDCKEL 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           D DFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKV+                 QMN
Sbjct: 61  DADFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVI-----------------QMN 120

Query: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180
           IQEDIRKQIVQELKINQAAQCPH+VVCYHSFYHNGAISLVLEYMDRGSLADV+RQVKTIL
Sbjct: 121 IQEDIRKQIVQELKINQAAQCPHIVVCYHSFYHNGAISLVLEYMDRGSLADVIRQVKTIL 180

Query: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQR 240
           EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNH+GEVKITDFGVSAMLASS+GQR
Sbjct: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAMLASSMGQR 240

Query: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300
           DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE
Sbjct: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300

Query: 301 AIVAKPSPSAPPDQFSPEFCSFVSA 326
           AIVAKP PSAPPDQFSPEFCSFVSA
Sbjct: 301 AIVAKPPPSAPPDQFSPEFCSFVSA 308

BLAST of Sgr022320 vs. NCBI nr
Match: KAG7021309.1 (Mitogen-activated protein kinase kinase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587.4 bits (1513), Expect = 7.5e-164
Identity = 304/376 (80.85%), Postives = 316/376 (84.04%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVPAQE +I SFLTASGTFHDGNLLLNQKGLRL+SEEKE QTSDCKE+
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGH----- 120
           DVDFSLEDLETVKVIGKGSGGVVQLV+HKWVGKLFALKVVI +EQ YT+AFV GH     
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120

Query: 121 ----------------------------------------------CMQMNIQEDIRKQI 180
                                                          +QMNIQEDIRKQI
Sbjct: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPH+VVC+HSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNH+G+VKITDFGVSAMLASS+GQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPSPS 326
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKP PS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360

BLAST of Sgr022320 vs. NCBI nr
Match: TYK10751.1 (mitogen-activated protein kinase kinase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 585.9 bits (1509), Expect = 2.2e-163
Identity = 297/326 (91.10%), Postives = 303/326 (92.94%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVP QE +I SFLTASGTFHDGNLLLNQKGLRLISEEKE+QT+D KEL
Sbjct: 1   MKTKTPLKQLKLSVPVQETSIRSFLTASGTFHDGNLLLNQKGLRLISEEKESQTTDSKEL 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGK FALKVVI                QMN
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKFFALKVVI----------------QMN 120

Query: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180
           IQEDIRKQIVQELKINQAAQC H+VVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL
Sbjct: 121 IQEDIRKQIVQELKINQAAQCSHIVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180

Query: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQR 240
           EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNH+GEVKITDFGVSAMLASS+GQR
Sbjct: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAMLASSMGQR 240

Query: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300
           DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE
Sbjct: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300

Query: 301 AIVAKPSPSAPPDQFSPEFCSFVSAW 327
           AIVAKP PSAPPDQFSPEFCSFVSAW
Sbjct: 301 AIVAKPPPSAPPDQFSPEFCSFVSAW 310

BLAST of Sgr022320 vs. NCBI nr
Match: KAA0061521.1 (mitogen-activated protein kinase kinase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 585.9 bits (1509), Expect = 2.2e-163
Identity = 297/326 (91.10%), Postives = 303/326 (92.94%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVP QE +I SFLTASGTFHDGNLLLNQKGLRLISEEKE+QT+D KEL
Sbjct: 3   MKTKTPLKQLKLSVPVQETSIRSFLTASGTFHDGNLLLNQKGLRLISEEKESQTTDSKEL 62

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGK FALKVVI                QMN
Sbjct: 63  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKFFALKVVI----------------QMN 122

Query: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180
           IQEDIRKQIVQELKINQAAQC H+VVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL
Sbjct: 123 IQEDIRKQIVQELKINQAAQCSHIVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 182

Query: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQR 240
           EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNH+GEVKITDFGVSAMLASS+GQR
Sbjct: 183 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAMLASSMGQR 242

Query: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300
           DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE
Sbjct: 243 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 302

Query: 301 AIVAKPSPSAPPDQFSPEFCSFVSAW 327
           AIVAKP PSAPPDQFSPEFCSFVSAW
Sbjct: 303 AIVAKPPPSAPPDQFSPEFCSFVSAW 312

BLAST of Sgr022320 vs. ExPASy Swiss-Prot
Match: Q9FJV0 (Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK6 PE=1 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 6.9e-144
Identity = 261/326 (80.06%), Postives = 285/326 (87.42%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           +K K+ LKQLKLSVPAQE+ I SFLTASGTFHDG+ LLNQKGLRL S+EK+++ SD KEL
Sbjct: 2   VKIKSNLKQLKLSVPAQESPISSFLTASGTFHDGDFLLNQKGLRLTSDEKQSRQSDSKEL 61

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           D + + EDLETVKVIGKGSGGVVQLVRHKWVGK FA+KV+                 QMN
Sbjct: 62  DFEITAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMKVI-----------------QMN 121

Query: 121 IQEDIRKQIVQELKINQA-AQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTI 180
           IQE+IRKQIVQELKINQA +QCPHVVVCYHSFYHNGA SLVLEYMDRGSLADV+RQVKTI
Sbjct: 122 IQEEIRKQIVQELKINQASSQCPHVVVCYHSFYHNGAFSLVLEYMDRGSLADVIRQVKTI 181

Query: 181 LEPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQ 240
           LEPYLAVVCKQVL GLVYLH+ERHVIHRDIKPSNLLVNH+GEVKI+DFGVSA LASS+GQ
Sbjct: 182 LEPYLAVVCKQVLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQ 241

Query: 241 RDTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELL 300
           RDTFVGTYNYMSPERISG TYDYSSDIWSLG+ VLECAIGRFPYL+SE+QQ+ PSFYELL
Sbjct: 242 RDTFVGTYNYMSPERISGSTYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELL 301

Query: 301 EAIVAKPSPSAPPDQFSPEFCSFVSA 326
            AIV  P P+AP DQFSPEFCSFVSA
Sbjct: 302 AAIVENPPPTAPSDQFSPEFCSFVSA 310

BLAST of Sgr022320 vs. ExPASy Swiss-Prot
Match: Q5QN75 (Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MKK1 PE=1 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 3.1e-128
Identity = 233/327 (71.25%), Postives = 270/327 (82.57%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEE--KEAQTSDCK 60
           M+ K P K+LKLSVPAQE  +  FLTASGTF DG L LNQ+GL+LISEE   E Q+++ K
Sbjct: 1   MRGKKPHKELKLSVPAQETPVDKFLTASGTFKDGELRLNQRGLQLISEETADEPQSTNLK 60

Query: 61  ELDVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQ 120
             DV  S++DLE ++VIGKGSGG+VQLVRHKWVG L+ALK +                 Q
Sbjct: 61  VEDVQLSMDDLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGI-----------------Q 120

Query: 121 MNIQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKT 180
           MNIQE +RKQIVQELKINQA Q  H+V+C+ SFYHNG I LVLEYMDRGSLAD+++QVKT
Sbjct: 121 MNIQEAVRKQIVQELKINQATQNAHIVLCHQSFYHNGVIYLVLEYMDRGSLADIIKQVKT 180

Query: 181 ILEPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIG 240
           ILEPYLAV+CKQVL+GL+YLHHERHVIHRDIKPSNLLVN +GEVKITDFGVSA+LASS+G
Sbjct: 181 ILEPYLAVLCKQVLEGLLYLHHERHVIHRDIKPSNLLVNRKGEVKITDFGVSAVLASSMG 240

Query: 241 QRDTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYEL 300
           QRDTFVGTYNYM+PERISG +YDY SDIWSLGLV+LECAIGRFPY+ S E + W SFYEL
Sbjct: 241 QRDTFVGTYNYMAPERISGSSYDYKSDIWSLGLVILECAIGRFPYIPS-EGEGWLSFYEL 300

Query: 301 LEAIVAKPSPSAPPDQFSPEFCSFVSA 326
           LEAIV +P PSAP DQFSPEFC+F+S+
Sbjct: 301 LEAIVDQPPPSAPADQFSPEFCAFISS 309

BLAST of Sgr022320 vs. ExPASy Swiss-Prot
Match: A9RWC9 (Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MKK1a PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.2e-105
Identity = 193/318 (60.69%), Postives = 245/318 (77.04%), Query Frame = 0

Query: 10  LKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKELDVDFSLEDL 69
           L+++VP + A I  ++TASGTF DG+++LN++G+R++S   + QTS     + + SL DL
Sbjct: 15  LRVNVPKENA-ISDYVTASGTFQDGDIMLNKEGIRVVS---QTQTS-TPAANGNISLSDL 74

Query: 70  ETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMNIQEDIRKQI 129
           ETVKV+GKGSGGVVQLVRHKW  + +ALKV+                  MNI+E  RKQI
Sbjct: 75  ETVKVLGKGSGGVVQLVRHKWTNETYALKVI-----------------HMNIEETTRKQI 134

Query: 130 VQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQV--KTILEPYLAVV 189
           VQELKIN A+QCP+VV+CYH+FY+NG IS+VLEYMD GSLAD+++++  K I EP L VV
Sbjct: 135 VQELKINHASQCPYVVICYHAFYNNGLISIVLEYMDGGSLADIMKELEPKCIKEPNLGVV 194

Query: 190 CKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQRDTFVGTY 249
           CKQVL GL+YLH  RH+IHRDIKPSNLLVNH+GEVKI+DFGVSA+LA+S+  RDTFVGT 
Sbjct: 195 CKQVLLGLMYLHQTRHIIHRDIKPSNLLVNHKGEVKISDFGVSAVLANSMAVRDTFVGTC 254

Query: 250 NYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPS 309
            YMSPER+ GGTY + SDIWSLGL +LECA+G++PY     ++ W +FYELL+ IV +P 
Sbjct: 255 TYMSPERVLGGTYGFDSDIWSLGLTLLECALGKYPYQPPGSEEGWMNFYELLQTIVDQPP 310

Query: 310 PSAPPDQFSPEFCSFVSA 326
           P AP DQFSPEFCSF+SA
Sbjct: 315 PVAPADQFSPEFCSFISA 310

BLAST of Sgr022320 vs. ExPASy Swiss-Prot
Match: A9SR33 (Mitogen-activated protein kinase kinase 1b OS=Physcomitrium patens OX=3218 GN=MKK1b PE=2 SV=2)

HSP 1 Score: 381.7 bits (979), Expect = 8.2e-105
Identity = 187/318 (58.81%), Postives = 236/318 (74.21%), Query Frame = 0

Query: 10  LKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKELDVDFSLEDL 69
           L+++VP QE +IH FLTA+G FHD ++ L+  GLR++S E    T+     D   SL DL
Sbjct: 10  LRVAVPKQENSIHRFLTANGVFHDDDIQLDHMGLRVVSSE---STAYANPPDAQLSLADL 69

Query: 70  ETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMNIQEDIRKQI 129
           E V+V+GKG+GG VQLVRHKW   ++ALK +                 QMNI E +RKQI
Sbjct: 70  EAVRVLGKGAGGSVQLVRHKWTNDIYALKGI-----------------QMNINETVRKQI 129

Query: 130 VQELKINQAA--QCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVV 189
           VQELKINQ    QCP++V CYHSFYHNG IS+VLEYMDRGSLAD+++Q K I EPYLAV+
Sbjct: 130 VQELKINQLTLHQCPYIVKCYHSFYHNGIISIVLEYMDRGSLADIIKQTKQIPEPYLAVI 189

Query: 190 CKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQRDTFVGTY 249
             QVL+GL YLH  RH+IHRDIKPSNLL+N +GEVKI+DFGVSA+L SS+ QRDTFVGTY
Sbjct: 190 SNQVLKGLNYLHQVRHIIHRDIKPSNLLINQKGEVKISDFGVSAVLISSMAQRDTFVGTY 249

Query: 250 NYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPS 309
            YMSPER+ G +Y Y SDIWSLGL +LECA+G FPY    +++ W +F+ L+E ++ +P 
Sbjct: 250 TYMSPERLGGQSYAYDSDIWSLGLTILECALGYFPYRPPGQEEGWNNFFMLMELVINQPP 307

Query: 310 PSAPPDQFSPEFCSFVSA 326
            +APPD+FSPEFCSF++A
Sbjct: 310 VAAPPDKFSPEFCSFIAA 307

BLAST of Sgr022320 vs. ExPASy Swiss-Prot
Match: A9S5R3 (Mitogen-activated protein kinase kinase 1c OS=Physcomitrium patens OX=3218 GN=MKK1c PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.6e-100
Identity = 180/319 (56.43%), Postives = 229/319 (71.79%), Query Frame = 0

Query: 10  LKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQT-SDCKELDVDFSLED 69
           L+++VP QE  +  FLTASGTF D ++ LN  GLR++S E    T +     D   S+ D
Sbjct: 10  LRVAVPKQETPVSKFLTASGTFQDDDIKLNHTGLRVVSSEPNLPTQTQSSSPDGQLSIAD 69

Query: 70  LETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMNIQEDIRKQ 129
           LE V+ +GKG+GG VQLVRHKW    +ALK +                 QMNI E +RKQ
Sbjct: 70  LELVRFLGKGAGGTVQLVRHKWTNVNYALKAI-----------------QMNINETVRKQ 129

Query: 130 IVQELKINQAA--QCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAV 189
           IVQELKINQ    QCP++V C+HSFYHNG IS++LEYMDRGSL+D+++Q K I EPYLAV
Sbjct: 130 IVQELKINQVTHQQCPYIVECFHSFYHNGVISMILEYMDRGSLSDIIKQQKQIPEPYLAV 189

Query: 190 VCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQRDTFVGT 249
           +  QVL+GL YLH  RH+IHRDIKPSNLL+NH+GEVKI+DFGVSA+L  S+ QRDTFVGT
Sbjct: 190 IASQVLKGLEYLHQVRHIIHRDIKPSNLLINHKGEVKISDFGVSAVLVHSLAQRDTFVGT 249

Query: 250 YNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKP 309
             YMSPER+ G +Y Y SD+WSLGL +LECA+G FPY  +  ++ W +F+ L+E IV +P
Sbjct: 250 CTYMSPERLQGRSYAYDSDLWSLGLTLLECALGTFPYKPAGMEEGWQNFFILMECIVNQP 309

Query: 310 SPSAPPDQFSPEFCSFVSA 326
             +A PD+FSPEFCSF+ +
Sbjct: 310 PAAASPDKFSPEFCSFIES 311

BLAST of Sgr022320 vs. ExPASy TrEMBL
Match: A0A6J1DPB3 (mitogen-activated protein kinase kinase 6 OS=Momordica charantia OX=3673 GN=LOC111021852 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.3e-165
Identity = 300/325 (92.31%), Postives = 304/325 (93.54%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVPAQEA IHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSD KEL
Sbjct: 1   MKTKTPLKQLKLSVPAQEANIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDSKEL 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           D DFSLEDLETVKVIGKGSGGVVQLV+HKWVGKLFALKV+                 QMN
Sbjct: 61  DADFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVI-----------------QMN 120

Query: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180
           IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL
Sbjct: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180

Query: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQR 240
           EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNH+GEVKITDFGVSAMLASS+GQR
Sbjct: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAMLASSMGQR 240

Query: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300
           DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE
Sbjct: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300

Query: 301 AIVAKPSPSAPPDQFSPEFCSFVSA 326
           AIVAKP PSAPPDQFSPEFCSFVSA
Sbjct: 301 AIVAKPPPSAPPDQFSPEFCSFVSA 308

BLAST of Sgr022320 vs. ExPASy TrEMBL
Match: A0A5A7V706 (Mitogen-activated protein kinase kinase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00820 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.1e-163
Identity = 297/326 (91.10%), Postives = 303/326 (92.94%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVP QE +I SFLTASGTFHDGNLLLNQKGLRLISEEKE+QT+D KEL
Sbjct: 3   MKTKTPLKQLKLSVPVQETSIRSFLTASGTFHDGNLLLNQKGLRLISEEKESQTTDSKEL 62

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGK FALKVVI                QMN
Sbjct: 63  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKFFALKVVI----------------QMN 122

Query: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180
           IQEDIRKQIVQELKINQAAQC H+VVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL
Sbjct: 123 IQEDIRKQIVQELKINQAAQCSHIVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 182

Query: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQR 240
           EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNH+GEVKITDFGVSAMLASS+GQR
Sbjct: 183 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAMLASSMGQR 242

Query: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300
           DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE
Sbjct: 243 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 302

Query: 301 AIVAKPSPSAPPDQFSPEFCSFVSAW 327
           AIVAKP PSAPPDQFSPEFCSFVSAW
Sbjct: 303 AIVAKPPPSAPPDQFSPEFCSFVSAW 312

BLAST of Sgr022320 vs. ExPASy TrEMBL
Match: A0A5D3CG31 (Mitogen-activated protein kinase kinase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00640 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.1e-163
Identity = 297/326 (91.10%), Postives = 303/326 (92.94%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVP QE +I SFLTASGTFHDGNLLLNQKGLRLISEEKE+QT+D KEL
Sbjct: 1   MKTKTPLKQLKLSVPVQETSIRSFLTASGTFHDGNLLLNQKGLRLISEEKESQTTDSKEL 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGK FALKVVI                QMN
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKFFALKVVI----------------QMN 120

Query: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180
           IQEDIRKQIVQELKINQAAQC H+VVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL
Sbjct: 121 IQEDIRKQIVQELKINQAAQCSHIVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180

Query: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQR 240
           EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNH+GEVKITDFGVSAMLASS+GQR
Sbjct: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAMLASSMGQR 240

Query: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300
           DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE
Sbjct: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300

Query: 301 AIVAKPSPSAPPDQFSPEFCSFVSAW 327
           AIVAKP PSAPPDQFSPEFCSFVSAW
Sbjct: 301 AIVAKPPPSAPPDQFSPEFCSFVSAW 310

BLAST of Sgr022320 vs. ExPASy TrEMBL
Match: A0A6J1FAH3 (mitogen-activated protein kinase kinase 6-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443886 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.6e-162
Identity = 293/325 (90.15%), Postives = 303/325 (93.23%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVPAQE +I SFLTASGTFHDGNLLLNQKGLRL+SEEKE QTSDCKE+
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           DVDFSLEDLETVKVIGKGSGGVVQLV+HKWVGKLFALKV+                 QMN
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVI-----------------QMN 120

Query: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180
           IQEDIRKQIVQELKINQAAQCPH+VVC+HSFYHNGAISLVLEYMDRGSLADVVRQVKTIL
Sbjct: 121 IQEDIRKQIVQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180

Query: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQR 240
           EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNH+G+VKITDFGVSAMLASS+GQR
Sbjct: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQR 240

Query: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300
           DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE
Sbjct: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300

Query: 301 AIVAKPSPSAPPDQFSPEFCSFVSA 326
           AIVAKP PSAP DQFSPEFCSFVSA
Sbjct: 301 AIVAKPPPSAPSDQFSPEFCSFVSA 308

BLAST of Sgr022320 vs. ExPASy TrEMBL
Match: A0A6J1FGT3 (mitogen-activated protein kinase kinase 6-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443886 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.6e-162
Identity = 293/325 (90.15%), Postives = 303/325 (93.23%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           MKTKTPLKQLKLSVPAQE +I SFLTASGTFHDGNLLLNQKGLRL+SEEKE QTSDCKE+
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           DVDFSLEDLETVKVIGKGSGGVVQLV+HKWVGKLFALKV+                 QMN
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVI-----------------QMN 120

Query: 121 IQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180
           IQEDIRKQIVQELKINQAAQCPH+VVC+HSFYHNGAISLVLEYMDRGSLADVVRQVKTIL
Sbjct: 121 IQEDIRKQIVQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTIL 180

Query: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQR 240
           EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNH+G+VKITDFGVSAMLASS+GQR
Sbjct: 181 EPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQR 240

Query: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300
           DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE
Sbjct: 241 DTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLE 300

Query: 301 AIVAKPSPSAPPDQFSPEFCSFVSA 326
           AIVAKP PSAP DQFSPEFCSFVSA
Sbjct: 301 AIVAKPPPSAPSDQFSPEFCSFVSA 308

BLAST of Sgr022320 vs. TAIR 10
Match: AT5G56580.1 (MAP kinase kinase 6 )

HSP 1 Score: 511.5 bits (1316), Expect = 4.9e-145
Identity = 261/326 (80.06%), Postives = 285/326 (87.42%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKEL 60
           +K K+ LKQLKLSVPAQE+ I SFLTASGTFHDG+ LLNQKGLRL S+EK+++ SD KEL
Sbjct: 2   VKIKSNLKQLKLSVPAQESPISSFLTASGTFHDGDFLLNQKGLRLTSDEKQSRQSDSKEL 61

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMN 120
           D + + EDLETVKVIGKGSGGVVQLVRHKWVGK FA+KV+                 QMN
Sbjct: 62  DFEITAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMKVI-----------------QMN 121

Query: 121 IQEDIRKQIVQELKINQA-AQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTI 180
           IQE+IRKQIVQELKINQA +QCPHVVVCYHSFYHNGA SLVLEYMDRGSLADV+RQVKTI
Sbjct: 122 IQEEIRKQIVQELKINQASSQCPHVVVCYHSFYHNGAFSLVLEYMDRGSLADVIRQVKTI 181

Query: 181 LEPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQ 240
           LEPYLAVVCKQVL GLVYLH+ERHVIHRDIKPSNLLVNH+GEVKI+DFGVSA LASS+GQ
Sbjct: 182 LEPYLAVVCKQVLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQ 241

Query: 241 RDTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELL 300
           RDTFVGTYNYMSPERISG TYDYSSDIWSLG+ VLECAIGRFPYL+SE+QQ+ PSFYELL
Sbjct: 242 RDTFVGTYNYMSPERISGSTYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELL 301

Query: 301 EAIVAKPSPSAPPDQFSPEFCSFVSA 326
            AIV  P P+AP DQFSPEFCSFVSA
Sbjct: 302 AAIVENPPPTAPSDQFSPEFCSFVSA 310

BLAST of Sgr022320 vs. TAIR 10
Match: AT4G29810.1 (MAP kinase kinase 2 )

HSP 1 Score: 366.3 bits (939), Expect = 2.5e-101
Identity = 184/316 (58.23%), Postives = 231/316 (73.10%), Query Frame = 0

Query: 10  LKLSVP-AQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKELDVDFSLED 69
           LKL++P A E +I  FLT SGTF DG+L +N+ G+R+IS+ +    S  K  D   SL D
Sbjct: 10  LKLAIPVAGEQSITKFLTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIKPADDQLSLSD 69

Query: 70  LETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMNIQEDIRKQ 129
           L+ VKVIGKGS GVVQLV+HKW G+ FALKV+                 Q+NI E IRK 
Sbjct: 70  LDMVKVIGKGSSGVVQLVQHKWTGQFFALKVI-----------------QLNIDEAIRKA 129

Query: 130 IVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVC 189
           I QELKINQ++QCP++V  Y SFY NGAISL+LEYMD GSLAD ++ VK I + YL+ + 
Sbjct: 130 IAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIF 189

Query: 190 KQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQRDTFVGTYN 249
           +QVLQGL+YLHH+RH+IHRD+KPSNLL+NH+GEVKITDFGVS ++ ++ G  +TFVGTYN
Sbjct: 190 RQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYN 249

Query: 250 YMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPSP 309
           YMSPERI G  Y   SDIWSLGLVVLECA G+FPY    ++++W S +EL+EAIV +P P
Sbjct: 250 YMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPP 308

Query: 310 SAPPDQFSPEFCSFVS 325
           + P   FSPE  SF+S
Sbjct: 310 ALPSGNFSPELSSFIS 308

BLAST of Sgr022320 vs. TAIR 10
Match: AT4G29810.2 (MAP kinase kinase 2 )

HSP 1 Score: 358.6 bits (919), Expect = 5.3e-99
Identity = 184/325 (56.62%), Postives = 231/325 (71.08%), Query Frame = 0

Query: 10  LKLSVP-AQEATIHSFL---------TASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKE 69
           LKL++P A E +I  FL         T SGTF DG+L +N+ G+R+IS+ +    S  K 
Sbjct: 10  LKLAIPVAGEQSITKFLRKGFGSLCRTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIKP 69

Query: 70  LDVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQM 129
            D   SL DL+ VKVIGKGS GVVQLV+HKW G+ FALKV+                 Q+
Sbjct: 70  ADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVI-----------------QL 129

Query: 130 NIQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTI 189
           NI E IRK I QELKINQ++QCP++V  Y SFY NGAISL+LEYMD GSLAD ++ VK I
Sbjct: 130 NIDEAIRKAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAI 189

Query: 190 LEPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQ 249
            + YL+ + +QVLQGL+YLHH+RH+IHRD+KPSNLL+NH+GEVKITDFGVS ++ ++ G 
Sbjct: 190 PDSYLSAIFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGL 249

Query: 250 RDTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELL 309
            +TFVGTYNYMSPERI G  Y   SDIWSLGLVVLECA G+FPY    ++++W S +EL+
Sbjct: 250 ANTFVGTYNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELM 309

Query: 310 EAIVAKPSPSAPPDQFSPEFCSFVS 325
           EAIV +P P+ P   FSPE  SF+S
Sbjct: 310 EAIVDQPPPALPSGNFSPELSSFIS 317

BLAST of Sgr022320 vs. TAIR 10
Match: AT4G29810.3 (MAP kinase kinase 2 )

HSP 1 Score: 358.6 bits (919), Expect = 5.3e-99
Identity = 184/325 (56.62%), Postives = 231/325 (71.08%), Query Frame = 0

Query: 10  LKLSVP-AQEATIHSFL---------TASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKE 69
           LKL++P A E +I  FL         T SGTF DG+L +N+ G+R+IS+ +    S  K 
Sbjct: 10  LKLAIPVAGEQSITKFLRKGFGSLCRTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIKP 69

Query: 70  LDVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQM 129
            D   SL DL+ VKVIGKGS GVVQLV+HKW G+ FALKV+                 Q+
Sbjct: 70  ADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVI-----------------QL 129

Query: 130 NIQEDIRKQIVQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTI 189
           NI E IRK I QELKINQ++QCP++V  Y SFY NGAISL+LEYMD GSLAD ++ VK I
Sbjct: 130 NIDEAIRKAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAI 189

Query: 190 LEPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQ 249
            + YL+ + +QVLQGL+YLHH+RH+IHRD+KPSNLL+NH+GEVKITDFGVS ++ ++ G 
Sbjct: 190 PDSYLSAIFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGL 249

Query: 250 RDTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELL 309
            +TFVGTYNYMSPERI G  Y   SDIWSLGLVVLECA G+FPY    ++++W S +EL+
Sbjct: 250 ANTFVGTYNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELM 309

Query: 310 EAIVAKPSPSAPPDQFSPEFCSFVS 325
           EAIV +P P+ P   FSPE  SF+S
Sbjct: 310 EAIVDQPPPALPSGNFSPELSSFIS 317

BLAST of Sgr022320 vs. TAIR 10
Match: AT4G26070.1 (MAP kinase/ ERK kinase 1 )

HSP 1 Score: 341.3 bits (874), Expect = 8.7e-94
Identity = 167/315 (53.02%), Postives = 220/315 (69.84%), Query Frame = 0

Query: 12  LSVPAQEATIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDCKELDVDFSLEDLET 71
           + +P  E +I  FLT SGTF DG+L +N+ G++ +S  +       + LD   SL DLE 
Sbjct: 11  ICLPPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEV 70

Query: 72  VKVIGKGSGGVVQLVRHKWVGKLFALKVVIWVEQFYTLAFVSGHCMQMNIQEDIRKQIVQ 131
           +KVIGKGS G VQLV+HK   + FALKV+                 Q+N +E   + I Q
Sbjct: 71  IKVIGKGSSGNVQLVKHKLTQQFFALKVI-----------------QLNTEESTCRAISQ 130

Query: 132 ELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCKQV 191
           EL+IN ++QCP++V CY SFYHNG +S++LE+MD GSLAD++++V  + E  L+ +CK+V
Sbjct: 131 ELRINLSSQCPYLVSCYQSFYHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRV 190

Query: 192 LQGLVYLHHERHVIHRDIKPSNLLVNHQGEVKITDFGVSAMLASSIGQRDTFVGTYNYMS 251
           L+GL Y+HHER +IHRD+KPSNLL+NH+GEVKITDFGVS +L S+    ++FVGTY YMS
Sbjct: 191 LRGLCYIHHERRIIHRDLKPSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMS 250

Query: 252 PERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPSPSAP 311
           PERISG  Y   SDIWSLGLV+LECA G+FPY   E ++ W S YEL++AIV  P P AP
Sbjct: 251 PERISGSLYSNKSDIWSLGLVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAP 308

Query: 312 PDQFSPEFCSFVSAW 327
            + FSPEFCSF+S W
Sbjct: 311 SNLFSPEFCSFISQW 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154636.11.5e-16492.31mitogen-activated protein kinase kinase 6 [Momordica charantia][more]
XP_038890673.12.6e-16491.38mitogen-activated protein kinase kinase 6 [Benincasa hispida] >XP_038890674.1 mi... [more]
KAG7021309.17.5e-16480.85Mitogen-activated protein kinase kinase 6 [Cucurbita argyrosperma subsp. argyros... [more]
TYK10751.12.2e-16391.10mitogen-activated protein kinase kinase 6 [Cucumis melo var. makuwa][more]
KAA0061521.12.2e-16391.10mitogen-activated protein kinase kinase 6 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9FJV06.9e-14480.06Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q5QN753.1e-12871.25Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=399... [more]
A9RWC92.2e-10560.69Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MK... [more]
A9SR338.2e-10558.81Mitogen-activated protein kinase kinase 1b OS=Physcomitrium patens OX=3218 GN=MK... [more]
A9S5R31.6e-10056.43Mitogen-activated protein kinase kinase 1c OS=Physcomitrium patens OX=3218 GN=MK... [more]
Match NameE-valueIdentityDescription
A0A6J1DPB37.3e-16592.31mitogen-activated protein kinase kinase 6 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A5A7V7061.1e-16391.10Mitogen-activated protein kinase kinase 6 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A5D3CG311.1e-16391.10Mitogen-activated protein kinase kinase 6 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1FAH32.6e-16290.15mitogen-activated protein kinase kinase 6-like isoform X2 OS=Cucurbita moschata ... [more]
A0A6J1FGT32.6e-16290.15mitogen-activated protein kinase kinase 6-like isoform X1 OS=Cucurbita moschata ... [more]
Match NameE-valueIdentityDescription
AT5G56580.14.9e-14580.06MAP kinase kinase 6 [more]
AT4G29810.12.5e-10158.23MAP kinase kinase 2 [more]
AT4G29810.25.3e-9956.62MAP kinase kinase 2 [more]
AT4G29810.35.3e-9956.62MAP kinase kinase 2 [more]
AT4G26070.18.7e-9453.02MAP kinase/ ERK kinase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 69..319
e-value: 3.6E-55
score: 199.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 70..324
e-value: 8.3E-54
score: 182.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 69..326
score: 39.028934
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 38..165
e-value: 9.8E-25
score: 88.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 167..326
e-value: 6.7E-47
score: 162.1
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 1..325
NoneNo IPR availablePANTHERPTHR24361:SF816MITOGEN-ACTIVATED PROTEIN KINASE KINASE 6coord: 1..325
NoneNo IPR availableCDDcd06623PKc_MAPKK_plant_likecoord: 67..325
e-value: 1.1417E-132
score: 376.162
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 75..98
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 204..216
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 67..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr022320.1Sgr022320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002229 defense response to oomycetes
biological_process GO:0010311 lateral root formation
biological_process GO:0007112 male meiosis cytokinesis
biological_process GO:0000165 MAPK cascade
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0009524 phragmoplast
molecular_function GO:0005524 ATP binding
molecular_function GO:0004708 MAP kinase kinase activity
molecular_function GO:0051019 mitogen-activated protein kinase binding
molecular_function GO:0031435 mitogen-activated protein kinase kinase kinase binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity