Sgr022307 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr022307
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionzinc protease PQQL-like isoform X1
Locationtig00154107: 412290 .. 417134 (+)
RNA-Seq ExpressionSgr022307
SyntenySgr022307
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCCTCGTGCTTCTGCAACCATTGATGATTTAAAAAATGTAGTCGTGAATATCAATTGTCTTGAGAAAGAGAGAAGAATTCCTCCTTGGGATGAAGAACACATCCCCGAGGAAATTGTCAGTACCATGCCAGATCCAGGGTAATCTATAATTTTTATAACTCCCCTGCAACGTTATGTTGAAACATTGAAATCTCAAGACCCTTGGTTCTCGTGTCCTTACTATTTTGCCATCTAATTAGAATTTGTTTCAGAACTGTTTTTTAAATATTTTTTAATGAAAAAAATCAATAAAACTATGTTTGAATAACTATTTTTAAAAGTTCTTTTTCCTCTTTTATGTGTTCTCACATTTGACTTTTTTTTAGAATAAATAAAATGGTAAATCTTTCCAAAATTATGAGTTTCTAACTAGAGATTTCAACATATTCAATAACATTAAATTTACAAATTCTTCAATGCTAGTTCATAAAAATAATTTTGGAAAGATAATCAGTATACTATTTTAGATGTAGTTTCCCAAACAGGCCTCTGATGTTCCAATATTCAACTTATAGGTAGAAACATATACGAAGTGAGTAAAATGATGTAAAATAAAGAGTTTCACATTTTCTTGGTTATGTTTAATTCCTTATGCTAAACTCTTCACTTCATTAAGTTTTGAATCCAGATGTTCACCAGTGGCTATCATTTTTATAATGGCATGTGGTAACTACTACACTGTTAAAATAATGTTTCAGGAATATTCTGCAGCAGTTGGAATATCCAAATATTCGAGCTACTGAAATATTTCTATCAAATGGCATGCGAGTTTGCTACAAGTGTACAGACTTTCTTGATGACCAGGTACCTTTTTAATTTTGTTAAAACTTTTCCTCAGATGCAATGGTCCGCTATTCGAATTGTTCATCTTCTATTGCTGCACCCGAGTCTAAGTTTCTCCTGTAAGTCTGACATAAAATTTTGTCTTAACAGGTAATATTTACAGGGTTCTCTTATGGGGCCTTATCTGAACTCCCAGAGAGAGAGTATAGTTCATGCTCGATGGGTTCAACCATTGCTGGAGAAATTGGAGTGTTTGGTTATCGACCTTCTGTACTTATGGACATGCTGGCTGGTAAGAGGGCTGAAGTTGGTACAAAGCTTGGAGCATACATGAGAACCTTTTCTGGTGATTGTTCACCGTCTGATCTGGAAACTGCCCTGCAGGTTTCCTTTCTGTCCTCATCAAATTAAGTACCCCCAGCTCTCCATTACTTATTATAGCCTTTAAACGAAAGAACACAATAAAAATATTTGTTCCAACAGAACATGATATGACTGGCATTGCCCCCTTGAAGCTCCTCTAGTAATAATTTCTGGTTCATACCTAAACTTGCTATGTTTTCTTTTAAGATGATTTCCCAATTAAAGGATTTCTGTTGCTATTCCTTTTTTCTTTATCATACGTGGGGTTTTGCTATAACTAACTGACTTAGGTTGATGTTGTGCTTCCTGCTCGTGCATGGAAATACCTTTATCTCCAAGCAAGTCTGTTGCTCAATTTCAGTTCTGGGTCATCTATTTCTTATTTCATTCTCACTGCCCTTTACTTTTACGCTTATTGTTTGTAATGCATATGGTTTTACAGCTAATTGCAGATTATGAGGTAGTATTCCGTTATCAATACCCTATATGAGTGATAAAAAAAAAAAGAACTACCTTATCATGAAAAAAAAATCATAAGCAGCTGACTGTGCCTAGTCAATGAGGGCCTACAAATTTTTAATCTATAAATAAGCATTTGAACTATGTAGGAATTCAGTCATTGTTCTAGCAGCATATATCATGCTTCTAATTTAATGCTAATGTAATTTCAGAAGAATTAAAAATAAAGATTTTTCCCTTTTGACCATTTCTTGACATTTTGTCTCGTTACATGTCTTGAGCGCATTCTGTTTCTCTGCAGCTGGTTTATCAACTATTCACAACAAATGTGACACCAGGAGAGGAGGATGTCAAAATTGTTATGCAAATGGCAGAAGAAGCTGTTCGTGCTCAGGAGAGGGATCCTTATACTGCATTTGCAACCGTGTGAAGGAGCTCAATTATGGAAACTCCTACTTTTTTAGGGTATGCTTGTTACCGTGCTTTTAGATTTTTATTTTTTCCTATAATTTTAACTTTTTTATTTTTATTTCTTACTCTCCAGCCAATTAGGTTAAGTGACCTTCGAAAGGTTGATCCACAAAAGGCTTGTGAATATTTCAACAACTGTTTCAGAGATCCATCCAGTTTTACTGTTGTAATTGTTGGGAATATTAATCCTTCTATAGCACTTCCTTTAATCCAGCAGTATTTGGTACGTGGAGCTTTTTATTCAGTGTGCTTACACGCAGCAACATATAGTGTTCGTGATATTTAAGGTTCTGATTTTCCTAAGCAGGGTGGAATCCAAAGCCTCCTGAACCAGTTATGGACTTCAATCGTGACGATCTGAAAGGCTTGCCATTCACTTTTCCTACAAGCATAGTTCGGTATAGTTTTCTAGACCATGTATATGAATCCATTGATGGTAGACATAAAACATGTTTCCAAATGTGTGATATAATTAATCAGTGGGAGGGAGGGTAGTGAAAATAAAATTAAAATAGTGAACCAAATCATGTTTCACTCTGTTTCTCATCAATGGCTTTCATTTTCCCCTATATTGTTTCTTAAGGTGTTGCTTAGAAGTGGTCTAGTATGAATGTGCATGTTTTCTGGTTCGTACAGTTATTACATTCATTTGGGATTTCCTTTCTCTAAACATGTTTAATGTGCTTGCATTGTAGAGAAGTGGTATATAGCCCCATGGTTGAAGCTCAATGTTCAGTTCAGCTTTGCTTTCCTGTGGAGCTCAAAAATGGAACCATGGTATTTCTTTCCTTTTTATTGTCAATTGACAGTGTACATTGTAATTGAAGTGTGTCCTGTTTATTACTTTATTTTGTTTATCCAATGGTTTAGAACGTGTTTTGTTCAAAACAAAAATGGCAGGTTGAGGAAATTCATTTCGTAGGGTTTTTGAGCAAATTGCTTGAGACAAAAATGATGCAAGTTCTGCGTTTCAAGCATGGACAGGTGTTTATGCATCTCGTGGTATTGTGAGTATTTGTTTTTTTTTCCTATTCTTTTGCTTAATTCTTTGGTTACCTTTTTGTACCAGATCTATTCTGCTGGGGTTTCAGTATTCCTTGGAGGTAACAAGCCTTCAAGAATTGGTCCTGTTCGTGGTGATATTAGCATAAACTTTTCTTGTGATCCAGAAATCTCATCGAAGCTGGTTTATACATTATCACAATCAACCTTCTTTTACTTTAATTCCATTAGCAAATTTAGCTGACCTTCTTTGTGATCCTGAATTTTTTAAAAGGTTGATCTTGCTTTGGATGAAATATTACGTCTTCAAGAAGAAGGGCCTACGGATCAAGATGTTTCCACTGTCCTAGAGATTGAGCAAAGGGCCCATGAAAATGGACTGCAGGTTATTTTTCATTTATGAGAATTTATTATTACTACATGAAGGGTAAAATGAAAATAGCATTCCACTTCAAGATATGTTTAACAAAATCTCCTCGACACTTGTTTTCCAAATAAAAATCATAATCACATCTTTAGCAACAACTGTCATTTTGGTCAACATTTTTGTTAATGATTTATGGGGAGGGGAACCTGTGTTGCAGTGGCACGCGTGGATTTGTGTCCAGCCACATAACTTATGTGGCAATTATGTGACATAATTACCACAAAGCTCTCTCTTCTCACTCTATTTGAAATGATAGATTCCCAAATCTGAATAATATAGCTAATTGTGGATGATCGCTTGGCTCTTAACCCTTGTTTTGTTCTGTGAATCACAATCCTTAAATCACTCTCTTTCCCCCTATCTTTTTCTATCCCCTCCCCATTTGGTATGGATAATAAAACAAAAACAAAAGTACTTAGATGAAAGAGACTTTCGCCTTCAAATGAAAAAGGTCCTCTTGCAGATGTTTCAAGGTTATATTTTGCCCTTATTAACATGACTGCAGACGTGGTTAGAAGAGTTATGATCCATTTTGTTGCCAACTACAAGAATAAAAGGAGTTGGTTTTCAGTTGTTCCTAGTTTATATATGGGCAATATGCCTAGCCATGAGGAATTTTATGGTTCCTGTACAACCAGTTAACTGTTTTGGGTTTTGGCTACCAGGAAAATTATTACTGGCTGGACAGGATTTTACGCAGCTACCAGTCAAGGATATACTCCGGTGATGTTGGAACTTCTTTTGAGGTTGGTGGCATTTAATAAGTTTTTATCTCTCCCCTTTTTATCTGTCAATACTTAATGTTGCTCTCTATGCAAGTGGCTTTCACCACTTATGGTGTGTTTATTTATTAGGTAAGTTCTTGATGAATCGTTTATGTTTTACAGGATGTGATAAAAGTTCCAATCAGCATTTCATTATTTCCGTTTGATTTAGGAGAAATGAATGTAAATATGTTTATCTACCATGAGATAAATTGTTTAGTTTCACTATACAATGCTGTGCCATATTTTGGAATCTCAATATATAGGTAAAGATGGACCCTGACATTCTATTCTCATCATTTTCTGTAAATTTTTCTCGATGCAGATCCAGGATGAGGGACGTTTGAAGGTCAGAAATGCCTTGACACCATCAACAGCACAGTTGGCACTACGAAGGATACTGCCATTTCCTTGCACAAAACAATATACTGCAGTAGTTCTGTTGCCAAGATCATATCAGTTCAGAAAACTGAAATCATTCTCCAACTCGGTCTATCAAATCATGGCAGAGATGCAAAGGTTAGTTTCATAG

mRNA sequence

ATGGAGCCTCGTGCTTCTGCAACCATTGATGATTTAAAAAATGTAGTCGTGAATATCAATTGTCTTGAGAAAGAGAGAAGAATTCCTCCTTGGGATGAAGAACACATCCCCGAGGAAATTGTCAGTACCATGCCAGATCCAGGGAATATTCTGCAGCAGTTGGAATATCCAAATATTCGAGCTACTGAAATATTTCTATCAAATGGCATGCGAGTTTGCTACAAGTGTACAGACTTTCTTGATGACCAGGTAATATTTACAGGGTTCTCTTATGGGGCCTTATCTGAACTCCCAGAGAGAGAGTATAGTTCATGCTCGATGGGTTCAACCATTGCTGGAGAAATTGGAGTGTTTGGTTATCGACCTTCTGTACTTATGGACATGCTGGCTGGTAAGAGGGCTGAAGTTGGTACAAAGCTTGGAGCATACATGAGAACCTTTTCTGGTGATTGTTCACCGTCTGATCTGGAAACTGCCCTGCAGCTGGTTTATCAACTATTCACAACAAATGTGACACCAGGAGAGGAGGATGTCAAAATTGTTATGCAAATGGCAGAAGAAGCTGTTCGTGCTCAGGAGAGGGATCCTTATACTGCATTTGCAACCCCAATTAGGTTAAGTGACCTTCGAAAGGTTGATCCACAAAAGGCTTGTGAATATTTCAACAACTGTTTCAGAGATCCATCCAGTTTTACTGTTGTAATTGTTGGGAATATTAATCCTTCTATAGCACTTCCTTTAATCCAGCAGTATTTGCCTCCTGAACCAGTTATGGACTTCAATCGTGACGATCTGAAAGGCTTGCCATTCACTTTTCCTACAAGCATAGTTCGAGAAGTGGTATATAGCCCCATGGTTGAAGCTCAATGTTCAGTTCAGCTTTGCTTTCCTGTGGAGCTCAAAAATGGAACCATGGTTGAGGAAATTCATTTCGTAGGGTTTTTGAGCAAATTGCTTGAGACAAAAATGATGCAAGTTCTGCGTTTCAAGCATGGACAGATCTATTCTGCTGGGGTTTCAGTATTCCTTGGAGGTAACAAGCCTTCAAGAATTGGTCCTGTTCGTGGTGATATTAGCATAAACTTTTCTTGTGATCCAGAAATCTCATCGAAGCTGGTTGATCTTGCTTTGGATGAAATATTACGTCTTCAAGAAGAAGGGCCTACGGATCAAGATGTTTCCACTGTCCTAGAGATTGAGCAAAGGGCCCATGAAAATGGACTGCAGGAAAATTATTACTGGCTGGACAGGATTTTACGCAGCTACCAGTCAAGGATATACTCCGGTGATGTTGGAACTTCTTTTGAGATCCAGGATGAGGGACGTTTGAAGGTCAGAAATGCCTTGACACCATCAACAGCACAGTTGGCACTACGAAGGATACTGCCATTTCCTTGCACAAAACAATATACTGCAGTAGTTCTGTTGCCAAGATCATATCAGTTCAGAAAACTGAAATCATTCTCCAACTCGGTCTATCAAATCATGGCAGAGATGCAAAGGTTAGTTTCATAG

Coding sequence (CDS)

ATGGAGCCTCGTGCTTCTGCAACCATTGATGATTTAAAAAATGTAGTCGTGAATATCAATTGTCTTGAGAAAGAGAGAAGAATTCCTCCTTGGGATGAAGAACACATCCCCGAGGAAATTGTCAGTACCATGCCAGATCCAGGGAATATTCTGCAGCAGTTGGAATATCCAAATATTCGAGCTACTGAAATATTTCTATCAAATGGCATGCGAGTTTGCTACAAGTGTACAGACTTTCTTGATGACCAGGTAATATTTACAGGGTTCTCTTATGGGGCCTTATCTGAACTCCCAGAGAGAGAGTATAGTTCATGCTCGATGGGTTCAACCATTGCTGGAGAAATTGGAGTGTTTGGTTATCGACCTTCTGTACTTATGGACATGCTGGCTGGTAAGAGGGCTGAAGTTGGTACAAAGCTTGGAGCATACATGAGAACCTTTTCTGGTGATTGTTCACCGTCTGATCTGGAAACTGCCCTGCAGCTGGTTTATCAACTATTCACAACAAATGTGACACCAGGAGAGGAGGATGTCAAAATTGTTATGCAAATGGCAGAAGAAGCTGTTCGTGCTCAGGAGAGGGATCCTTATACTGCATTTGCAACCCCAATTAGGTTAAGTGACCTTCGAAAGGTTGATCCACAAAAGGCTTGTGAATATTTCAACAACTGTTTCAGAGATCCATCCAGTTTTACTGTTGTAATTGTTGGGAATATTAATCCTTCTATAGCACTTCCTTTAATCCAGCAGTATTTGCCTCCTGAACCAGTTATGGACTTCAATCGTGACGATCTGAAAGGCTTGCCATTCACTTTTCCTACAAGCATAGTTCGAGAAGTGGTATATAGCCCCATGGTTGAAGCTCAATGTTCAGTTCAGCTTTGCTTTCCTGTGGAGCTCAAAAATGGAACCATGGTTGAGGAAATTCATTTCGTAGGGTTTTTGAGCAAATTGCTTGAGACAAAAATGATGCAAGTTCTGCGTTTCAAGCATGGACAGATCTATTCTGCTGGGGTTTCAGTATTCCTTGGAGGTAACAAGCCTTCAAGAATTGGTCCTGTTCGTGGTGATATTAGCATAAACTTTTCTTGTGATCCAGAAATCTCATCGAAGCTGGTTGATCTTGCTTTGGATGAAATATTACGTCTTCAAGAAGAAGGGCCTACGGATCAAGATGTTTCCACTGTCCTAGAGATTGAGCAAAGGGCCCATGAAAATGGACTGCAGGAAAATTATTACTGGCTGGACAGGATTTTACGCAGCTACCAGTCAAGGATATACTCCGGTGATGTTGGAACTTCTTTTGAGATCCAGGATGAGGGACGTTTGAAGGTCAGAAATGCCTTGACACCATCAACAGCACAGTTGGCACTACGAAGGATACTGCCATTTCCTTGCACAAAACAATATACTGCAGTAGTTCTGTTGCCAAGATCATATCAGTTCAGAAAACTGAAATCATTCTCCAACTCGGTCTATCAAATCATGGCAGAGATGCAAAGGTTAGTTTCATAG

Protein sequence

MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIRATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGYRPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKIVMQMAEEAVRAQERDPYTAFATPIRLSDLRKVDPQKACEYFNNCFRDPSSFTVVIVGNINPSIALPLIQQYLPPEPVMDFNRDDLKGLPFTFPTSIVREVVYSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSVFLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQRAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRILPFPCTKQYTAVVLLPRSYQFRKLKSFSNSVYQIMAEMQRLVS
Homology
BLAST of Sgr022307 vs. NCBI nr
Match: XP_038890062.1 (zinc protease PQQL-like isoform X3 [Benincasa hispida])

HSP 1 Score: 923.7 bits (2386), Expect = 6.8e-265
Identity = 460/507 (90.73%), Postives = 479/507 (94.48%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           +EPRASATIDDLKNVV+NI+CLEKER IPPWDEEHIPEEIVSTMP+PGNILQQ EYPNI 
Sbjct: 298 IEPRASATIDDLKNVVMNISCLEKERSIPPWDEEHIPEEIVSTMPNPGNILQQQEYPNIG 357

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
           ATEIFLSNGMRVCYKCTDFLDDQV+FTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY
Sbjct: 358 ATEIFLSNGMRVCYKCTDFLDDQVVFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 417

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           RPSVLMD+LAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI
Sbjct: 418 RPSVLMDILAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 477

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEEAVRAQERDPYTAF                PIRLSDLRKVDPQ+ACEYFNNCFR
Sbjct: 478 VMQMAEEAVRAQERDPYTAFVNRVKELNYGNSYFFRPIRLSDLRKVDPQRACEYFNNCFR 537

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVVIVGNINPSIALPLIQQYL     PPEP+M FNRDDLKGLPFTFPTSIVREVV
Sbjct: 538 DPSNFTVVIVGNINPSIALPLIQQYLGGIPKPPEPIMKFNRDDLKGLPFTFPTSIVREVV 597

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
           YSPMVEAQCSVQLCFPVEL NGTMVEEIH+VGFLSKLLET+MMQVLRFKHGQIYSAGVSV
Sbjct: 598 YSPMVEAQCSVQLCFPVELTNGTMVEEIHYVGFLSKLLETRMMQVLRFKHGQIYSAGVSV 657

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS++LEIEQ
Sbjct: 658 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSILEIEQ 717

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENGLQENYYWLDRILRSYQSRIYSGDVG+SFEIQDEGRL VRN+LTP TAQLAL+RI
Sbjct: 718 RAHENGLQENYYWLDRILRSYQSRIYSGDVGSSFEIQDEGRLNVRNSLTPLTAQLALQRI 777

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
           LPFPCTKQYTAV+LLPRSY+FRKLKSF
Sbjct: 778 LPFPCTKQYTAVILLPRSYRFRKLKSF 804

BLAST of Sgr022307 vs. NCBI nr
Match: XP_038890061.1 (zinc protease PQQL-like isoform X2 [Benincasa hispida])

HSP 1 Score: 923.7 bits (2386), Expect = 6.8e-265
Identity = 460/507 (90.73%), Postives = 479/507 (94.48%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           +EPRASATIDDLKNVV+NI+CLEKER IPPWDEEHIPEEIVSTMP+PGNILQQ EYPNI 
Sbjct: 417 IEPRASATIDDLKNVVMNISCLEKERSIPPWDEEHIPEEIVSTMPNPGNILQQQEYPNIG 476

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
           ATEIFLSNGMRVCYKCTDFLDDQV+FTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY
Sbjct: 477 ATEIFLSNGMRVCYKCTDFLDDQVVFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 536

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           RPSVLMD+LAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI
Sbjct: 537 RPSVLMDILAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 596

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEEAVRAQERDPYTAF                PIRLSDLRKVDPQ+ACEYFNNCFR
Sbjct: 597 VMQMAEEAVRAQERDPYTAFVNRVKELNYGNSYFFRPIRLSDLRKVDPQRACEYFNNCFR 656

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVVIVGNINPSIALPLIQQYL     PPEP+M FNRDDLKGLPFTFPTSIVREVV
Sbjct: 657 DPSNFTVVIVGNINPSIALPLIQQYLGGIPKPPEPIMKFNRDDLKGLPFTFPTSIVREVV 716

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
           YSPMVEAQCSVQLCFPVEL NGTMVEEIH+VGFLSKLLET+MMQVLRFKHGQIYSAGVSV
Sbjct: 717 YSPMVEAQCSVQLCFPVELTNGTMVEEIHYVGFLSKLLETRMMQVLRFKHGQIYSAGVSV 776

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS++LEIEQ
Sbjct: 777 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSILEIEQ 836

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENGLQENYYWLDRILRSYQSRIYSGDVG+SFEIQDEGRL VRN+LTP TAQLAL+RI
Sbjct: 837 RAHENGLQENYYWLDRILRSYQSRIYSGDVGSSFEIQDEGRLNVRNSLTPLTAQLALQRI 896

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
           LPFPCTKQYTAV+LLPRSY+FRKLKSF
Sbjct: 897 LPFPCTKQYTAVILLPRSYRFRKLKSF 923

BLAST of Sgr022307 vs. NCBI nr
Match: XP_038890060.1 (zinc protease PQQL-like isoform X1 [Benincasa hispida])

HSP 1 Score: 923.7 bits (2386), Expect = 6.8e-265
Identity = 460/507 (90.73%), Postives = 479/507 (94.48%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           +EPRASATIDDLKNVV+NI+CLEKER IPPWDEEHIPEEIVSTMP+PGNILQQ EYPNI 
Sbjct: 465 IEPRASATIDDLKNVVMNISCLEKERSIPPWDEEHIPEEIVSTMPNPGNILQQQEYPNIG 524

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
           ATEIFLSNGMRVCYKCTDFLDDQV+FTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY
Sbjct: 525 ATEIFLSNGMRVCYKCTDFLDDQVVFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 584

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           RPSVLMD+LAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI
Sbjct: 585 RPSVLMDILAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 644

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEEAVRAQERDPYTAF                PIRLSDLRKVDPQ+ACEYFNNCFR
Sbjct: 645 VMQMAEEAVRAQERDPYTAFVNRVKELNYGNSYFFRPIRLSDLRKVDPQRACEYFNNCFR 704

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVVIVGNINPSIALPLIQQYL     PPEP+M FNRDDLKGLPFTFPTSIVREVV
Sbjct: 705 DPSNFTVVIVGNINPSIALPLIQQYLGGIPKPPEPIMKFNRDDLKGLPFTFPTSIVREVV 764

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
           YSPMVEAQCSVQLCFPVEL NGTMVEEIH+VGFLSKLLET+MMQVLRFKHGQIYSAGVSV
Sbjct: 765 YSPMVEAQCSVQLCFPVELTNGTMVEEIHYVGFLSKLLETRMMQVLRFKHGQIYSAGVSV 824

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS++LEIEQ
Sbjct: 825 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSILEIEQ 884

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENGLQENYYWLDRILRSYQSRIYSGDVG+SFEIQDEGRL VRN+LTP TAQLAL+RI
Sbjct: 885 RAHENGLQENYYWLDRILRSYQSRIYSGDVGSSFEIQDEGRLNVRNSLTPLTAQLALQRI 944

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
           LPFPCTKQYTAV+LLPRSY+FRKLKSF
Sbjct: 945 LPFPCTKQYTAVILLPRSYRFRKLKSF 971

BLAST of Sgr022307 vs. NCBI nr
Match: XP_022999427.1 (zinc protease PQQL-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 917.9 bits (2371), Expect = 3.7e-263
Identity = 457/507 (90.14%), Postives = 478/507 (94.28%), Query Frame = 0

Query: 1    MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
            +EPRASAT+D LKNVV+NIN LEKER IPPWDEEHIPEEIV+TMP+PGNILQQ EYPNI 
Sbjct: 503  IEPRASATVDGLKNVVMNINSLEKERSIPPWDEEHIPEEIVTTMPNPGNILQQQEYPNIG 562

Query: 61   ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
            ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY
Sbjct: 563  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 622

Query: 121  RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
            RPSVLMD+LAGKRAEVGTKLGAYMRTFSGDCSPSDLETA+QLVYQLFTTNVTPGEEDVKI
Sbjct: 623  RPSVLMDILAGKRAEVGTKLGAYMRTFSGDCSPSDLETAMQLVYQLFTTNVTPGEEDVKI 682

Query: 181  VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
            VMQMAEEAVRAQERDPYTAFA               PIRLSDL+KVDPQKACEYFNNCFR
Sbjct: 683  VMQMAEEAVRAQERDPYTAFANRVKELNYGNSYFFRPIRLSDLQKVDPQKACEYFNNCFR 742

Query: 241  DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
            DPS+FTVV+VGNINPSIALPLIQQYL     PPEP+M+FNRDDLKGLPFTFPTSIVREVV
Sbjct: 743  DPSNFTVVVVGNINPSIALPLIQQYLGGIPKPPEPIMNFNRDDLKGLPFTFPTSIVREVV 802

Query: 301  YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
            YSPMVEAQCSVQLCFPVEL NGTMVEEIHFVGFLSKLLET+MMQVLRFKHGQIYSAGVSV
Sbjct: 803  YSPMVEAQCSVQLCFPVELTNGTMVEEIHFVGFLSKLLETRMMQVLRFKHGQIYSAGVSV 862

Query: 361  FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
            FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS++LEIEQ
Sbjct: 863  FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSILEIEQ 922

Query: 421  RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
            RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRL VRN+LTP TAQLAL+RI
Sbjct: 923  RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLNVRNSLTPLTAQLALQRI 982

Query: 481  LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
            LPFPCTKQYTAV+LLP SY+F+KLKSF
Sbjct: 983  LPFPCTKQYTAVILLPSSYRFKKLKSF 1009

BLAST of Sgr022307 vs. NCBI nr
Match: XP_022999428.1 (zinc protease PQQL-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 917.9 bits (2371), Expect = 3.7e-263
Identity = 457/507 (90.14%), Postives = 478/507 (94.28%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           +EPRASAT+D LKNVV+NIN LEKER IPPWDEEHIPEEIV+TMP+PGNILQQ EYPNI 
Sbjct: 465 IEPRASATVDGLKNVVMNINSLEKERSIPPWDEEHIPEEIVTTMPNPGNILQQQEYPNIG 524

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
           ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY
Sbjct: 525 ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 584

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           RPSVLMD+LAGKRAEVGTKLGAYMRTFSGDCSPSDLETA+QLVYQLFTTNVTPGEEDVKI
Sbjct: 585 RPSVLMDILAGKRAEVGTKLGAYMRTFSGDCSPSDLETAMQLVYQLFTTNVTPGEEDVKI 644

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEEAVRAQERDPYTAFA               PIRLSDL+KVDPQKACEYFNNCFR
Sbjct: 645 VMQMAEEAVRAQERDPYTAFANRVKELNYGNSYFFRPIRLSDLQKVDPQKACEYFNNCFR 704

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVV+VGNINPSIALPLIQQYL     PPEP+M+FNRDDLKGLPFTFPTSIVREVV
Sbjct: 705 DPSNFTVVVVGNINPSIALPLIQQYLGGIPKPPEPIMNFNRDDLKGLPFTFPTSIVREVV 764

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
           YSPMVEAQCSVQLCFPVEL NGTMVEEIHFVGFLSKLLET+MMQVLRFKHGQIYSAGVSV
Sbjct: 765 YSPMVEAQCSVQLCFPVELTNGTMVEEIHFVGFLSKLLETRMMQVLRFKHGQIYSAGVSV 824

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS++LEIEQ
Sbjct: 825 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSILEIEQ 884

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRL VRN+LTP TAQLAL+RI
Sbjct: 885 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLNVRNSLTPLTAQLALQRI 944

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
           LPFPCTKQYTAV+LLP SY+F+KLKSF
Sbjct: 945 LPFPCTKQYTAVILLPSSYRFKKLKSF 971

BLAST of Sgr022307 vs. ExPASy Swiss-Prot
Match: Q9FJT9 (Zinc protease PQQL-like OS=Arabidopsis thaliana OX=3702 GN=At5g56730 PE=1 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.2e-200
Identity = 348/510 (68.24%), Postives = 410/510 (80.39%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           MEP+++ATID ++NVV  +N LE+E+ I PWDEE+IPEEIVS  P PG+I  QLEYP + 
Sbjct: 439 MEPKSAATIDHMRNVVSKVNSLEEEKMIAPWDEENIPEEIVSEKPTPGDITHQLEYPEVG 498

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
            TE+ LSNGM+VCYK TDFLDDQV+FTGFSYG LSELPE +Y SCSMGSTIAGEIG+FGY
Sbjct: 499 VTELTLSNGMQVCYKSTDFLDDQVLFTGFSYGGLSELPESDYISCSMGSTIAGEIGMFGY 558

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           +PSVLMDMLA                        DLETALQLVYQLFTTNV P EE+V I
Sbjct: 559 KPSVLMDMLA------------------------DLETALQLVYQLFTTNVMPQEEEVGI 618

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEE+VRA+ERDPYT FA               PIR+S+LRKVDP KACEYFN+CFR
Sbjct: 619 VMQMAEESVRARERDPYTVFANRVKELNYGNSYFFRPIRISELRKVDPLKACEYFNSCFR 678

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVVIVGN++P+IALPLI QYL     PP+PV++FNRDDLKGLPFTFPT I +E V
Sbjct: 679 DPSTFTVVIVGNLDPTIALPLILQYLGGIPKPPQPVLNFNRDDLKGLPFTFPTKITKEFV 738

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
            SPMVEAQCSVQLCFPV+L NGTM+EEIH +GFL KLLETK++Q LRF+HGQIYSA VSV
Sbjct: 739 RSPMVEAQCSVQLCFPVQLTNGTMIEEIHCIGFLGKLLETKIIQFLRFEHGQIYSAEVSV 798

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSR   +RGDIS+NFSCDPEISSKLVDLAL+EI+RLQ+EGP+ +D+S +LEIEQ
Sbjct: 799 FLGGNKPSRTADLRGDISVNFSCDPEISSKLVDLALEEIVRLQKEGPSQEDISAILEIEQ 858

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENG+QENYYWLDRI+R YQSR+Y+GD+G S +I +EGRL++R +L P TAQ AL+RI
Sbjct: 859 RAHENGMQENYYWLDRIIRGYQSRVYAGDLGASCKILEEGRLRMRESLAPQTAQAALQRI 918

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSFSNS 492
           LP PC KQYTAV+L+P+  +F  L S  +S
Sbjct: 919 LPHPCKKQYTAVILMPQRSRFGFLSSIFSS 924

BLAST of Sgr022307 vs. ExPASy TrEMBL
Match: A0A6J1KAU8 (zinc protease PQQL-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493804 PE=4 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.8e-263
Identity = 457/507 (90.14%), Postives = 478/507 (94.28%), Query Frame = 0

Query: 1    MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
            +EPRASAT+D LKNVV+NIN LEKER IPPWDEEHIPEEIV+TMP+PGNILQQ EYPNI 
Sbjct: 503  IEPRASATVDGLKNVVMNINSLEKERSIPPWDEEHIPEEIVTTMPNPGNILQQQEYPNIG 562

Query: 61   ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
            ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY
Sbjct: 563  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 622

Query: 121  RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
            RPSVLMD+LAGKRAEVGTKLGAYMRTFSGDCSPSDLETA+QLVYQLFTTNVTPGEEDVKI
Sbjct: 623  RPSVLMDILAGKRAEVGTKLGAYMRTFSGDCSPSDLETAMQLVYQLFTTNVTPGEEDVKI 682

Query: 181  VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
            VMQMAEEAVRAQERDPYTAFA               PIRLSDL+KVDPQKACEYFNNCFR
Sbjct: 683  VMQMAEEAVRAQERDPYTAFANRVKELNYGNSYFFRPIRLSDLQKVDPQKACEYFNNCFR 742

Query: 241  DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
            DPS+FTVV+VGNINPSIALPLIQQYL     PPEP+M+FNRDDLKGLPFTFPTSIVREVV
Sbjct: 743  DPSNFTVVVVGNINPSIALPLIQQYLGGIPKPPEPIMNFNRDDLKGLPFTFPTSIVREVV 802

Query: 301  YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
            YSPMVEAQCSVQLCFPVEL NGTMVEEIHFVGFLSKLLET+MMQVLRFKHGQIYSAGVSV
Sbjct: 803  YSPMVEAQCSVQLCFPVELTNGTMVEEIHFVGFLSKLLETRMMQVLRFKHGQIYSAGVSV 862

Query: 361  FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
            FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS++LEIEQ
Sbjct: 863  FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSILEIEQ 922

Query: 421  RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
            RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRL VRN+LTP TAQLAL+RI
Sbjct: 923  RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLNVRNSLTPLTAQLALQRI 982

Query: 481  LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
            LPFPCTKQYTAV+LLP SY+F+KLKSF
Sbjct: 983  LPFPCTKQYTAVILLPSSYRFKKLKSF 1009

BLAST of Sgr022307 vs. ExPASy TrEMBL
Match: A0A6J1KJP3 (zinc protease PQQL-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493804 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.8e-263
Identity = 457/507 (90.14%), Postives = 478/507 (94.28%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           +EPRASAT+D LKNVV+NIN LEKER IPPWDEEHIPEEIV+TMP+PGNILQQ EYPNI 
Sbjct: 465 IEPRASATVDGLKNVVMNINSLEKERSIPPWDEEHIPEEIVTTMPNPGNILQQQEYPNIG 524

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
           ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY
Sbjct: 525 ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 584

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           RPSVLMD+LAGKRAEVGTKLGAYMRTFSGDCSPSDLETA+QLVYQLFTTNVTPGEEDVKI
Sbjct: 585 RPSVLMDILAGKRAEVGTKLGAYMRTFSGDCSPSDLETAMQLVYQLFTTNVTPGEEDVKI 644

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEEAVRAQERDPYTAFA               PIRLSDL+KVDPQKACEYFNNCFR
Sbjct: 645 VMQMAEEAVRAQERDPYTAFANRVKELNYGNSYFFRPIRLSDLQKVDPQKACEYFNNCFR 704

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVV+VGNINPSIALPLIQQYL     PPEP+M+FNRDDLKGLPFTFPTSIVREVV
Sbjct: 705 DPSNFTVVVVGNINPSIALPLIQQYLGGIPKPPEPIMNFNRDDLKGLPFTFPTSIVREVV 764

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
           YSPMVEAQCSVQLCFPVEL NGTMVEEIHFVGFLSKLLET+MMQVLRFKHGQIYSAGVSV
Sbjct: 765 YSPMVEAQCSVQLCFPVELTNGTMVEEIHFVGFLSKLLETRMMQVLRFKHGQIYSAGVSV 824

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS++LEIEQ
Sbjct: 825 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSILEIEQ 884

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRL VRN+LTP TAQLAL+RI
Sbjct: 885 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLNVRNSLTPLTAQLALQRI 944

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
           LPFPCTKQYTAV+LLP SY+F+KLKSF
Sbjct: 945 LPFPCTKQYTAVILLPSSYRFKKLKSF 971

BLAST of Sgr022307 vs. ExPASy TrEMBL
Match: A0A6J1DPE6 (zinc protease PQQL-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021875 PE=3 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 3.8e-261
Identity = 459/507 (90.53%), Postives = 472/507 (93.10%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           +EPRASATIDDLKNVVVNINCLEKER IPPWDEEHIPEEIV + P  GNILQQLEYPNI 
Sbjct: 465 IEPRASATIDDLKNVVVNINCLEKERSIPPWDEEHIPEEIVISKPGLGNILQQLEYPNIG 524

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
           A+EIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREY+SCSMGSTIAGEIGVFGY
Sbjct: 525 ASEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYTSCSMGSTIAGEIGVFGY 584

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFT NVTPGEEDVKI
Sbjct: 585 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTKNVTPGEEDVKI 644

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEEAVRAQERDPYTAFA               PIRL DL+KVDPQKACEYFN CFR
Sbjct: 645 VMQMAEEAVRAQERDPYTAFANRVKELNYGNSYFFRPIRLRDLQKVDPQKACEYFNKCFR 704

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVVIVGNINPSIALPLIQQYL     PPEPVMDFNRDDLKGLPFTF T IVREVV
Sbjct: 705 DPSTFTVVIVGNINPSIALPLIQQYLGGIPKPPEPVMDFNRDDLKGLPFTFSTGIVREVV 764

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
           YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV
Sbjct: 765 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 824

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSR  PVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS+VLEIEQ
Sbjct: 825 FLGGNKPSRNDPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSVLEIEQ 884

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENGLQENYYWLDRILRSYQSRIYSGDVG SFEIQDEGRLKVRN+LTP TAQLAL+RI
Sbjct: 885 RAHENGLQENYYWLDRILRSYQSRIYSGDVGNSFEIQDEGRLKVRNSLTPLTAQLALQRI 944

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
           LPFPCTKQYTAV+LLPRS++FRKLKSF
Sbjct: 945 LPFPCTKQYTAVILLPRSFRFRKLKSF 971

BLAST of Sgr022307 vs. ExPASy TrEMBL
Match: A0A6J1DM98 (zinc protease PQQL-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021875 PE=4 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 3.8e-261
Identity = 459/507 (90.53%), Postives = 472/507 (93.10%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           +EPRASATIDDLKNVVVNINCLEKER IPPWDEEHIPEEIV + P  GNILQQLEYPNI 
Sbjct: 404 IEPRASATIDDLKNVVVNINCLEKERSIPPWDEEHIPEEIVISKPGLGNILQQLEYPNIG 463

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
           A+EIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREY+SCSMGSTIAGEIGVFGY
Sbjct: 464 ASEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYTSCSMGSTIAGEIGVFGY 523

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFT NVTPGEEDVKI
Sbjct: 524 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTKNVTPGEEDVKI 583

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEEAVRAQERDPYTAFA               PIRL DL+KVDPQKACEYFN CFR
Sbjct: 584 VMQMAEEAVRAQERDPYTAFANRVKELNYGNSYFFRPIRLRDLQKVDPQKACEYFNKCFR 643

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVVIVGNINPSIALPLIQQYL     PPEPVMDFNRDDLKGLPFTF T IVREVV
Sbjct: 644 DPSTFTVVIVGNINPSIALPLIQQYLGGIPKPPEPVMDFNRDDLKGLPFTFSTGIVREVV 703

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
           YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV
Sbjct: 704 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 763

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSR  PVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS+VLEIEQ
Sbjct: 764 FLGGNKPSRNDPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSVLEIEQ 823

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENGLQENYYWLDRILRSYQSRIYSGDVG SFEIQDEGRLKVRN+LTP TAQLAL+RI
Sbjct: 824 RAHENGLQENYYWLDRILRSYQSRIYSGDVGNSFEIQDEGRLKVRNSLTPLTAQLALQRI 883

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
           LPFPCTKQYTAV+LLPRS++FRKLKSF
Sbjct: 884 LPFPCTKQYTAVILLPRSFRFRKLKSF 910

BLAST of Sgr022307 vs. ExPASy TrEMBL
Match: A0A1S3C9Q7 (zinc protease PQQL-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498391 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.5e-260
Identity = 452/507 (89.15%), Postives = 474/507 (93.49%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           +EPRASATIDDLKNVV+NI CLEKER IPPWDEE+IPEEIVSTMP+PGNI+QQ EYPNI 
Sbjct: 464 IEPRASATIDDLKNVVMNITCLEKERSIPPWDEENIPEEIVSTMPNPGNIVQQKEYPNIG 523

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
           ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY
Sbjct: 524 ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 583

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           RPSVLMD+LAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI
Sbjct: 584 RPSVLMDILAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 643

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEEAVRAQERDPYTAFA               PIRL DL+KVDPQ+ACEYFN CFR
Sbjct: 644 VMQMAEEAVRAQERDPYTAFANRVKELNYGNSYFFRPIRLRDLKKVDPQRACEYFNKCFR 703

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVV+VGNINPSIALPLIQQYL     PPEP+M+FNRDDLKGLPF FPT IVREVV
Sbjct: 704 DPSNFTVVVVGNINPSIALPLIQQYLGGIPKPPEPIMNFNRDDLKGLPFKFPTRIVREVV 763

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
           YSPMVEAQCSVQLCFPVEL NGTMVEEIH+VGFLSKLLET+MMQVLRFKHGQIYSAGVSV
Sbjct: 764 YSPMVEAQCSVQLCFPVELTNGTMVEEIHYVGFLSKLLETRMMQVLRFKHGQIYSAGVSV 823

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSR GPVRGDISINFSCDPEISSKLVDLAL+EILRLQEEGPTDQDVS++LEIEQ
Sbjct: 824 FLGGNKPSRSGPVRGDISINFSCDPEISSKLVDLALNEILRLQEEGPTDQDVSSILEIEQ 883

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENGLQENYYWLDRILRSYQSRIYSGDVG+SFEIQDEGRL VRN+LTP TAQLAL+RI
Sbjct: 884 RAHENGLQENYYWLDRILRSYQSRIYSGDVGSSFEIQDEGRLNVRNSLTPLTAQLALQRI 943

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSF 489
           LPFPCTKQYTAV+LLP SY+FRKLKSF
Sbjct: 944 LPFPCTKQYTAVILLPASYRFRKLKSF 970

BLAST of Sgr022307 vs. TAIR 10
Match: AT5G56730.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 700.7 bits (1807), Expect = 8.8e-202
Identity = 348/510 (68.24%), Postives = 410/510 (80.39%), Query Frame = 0

Query: 1   MEPRASATIDDLKNVVVNINCLEKERRIPPWDEEHIPEEIVSTMPDPGNILQQLEYPNIR 60
           MEP+++ATID ++NVV  +N LE+E+ I PWDEE+IPEEIVS  P PG+I  QLEYP + 
Sbjct: 439 MEPKSAATIDHMRNVVSKVNSLEEEKMIAPWDEENIPEEIVSEKPTPGDITHQLEYPEVG 498

Query: 61  ATEIFLSNGMRVCYKCTDFLDDQVIFTGFSYGALSELPEREYSSCSMGSTIAGEIGVFGY 120
            TE+ LSNGM+VCYK TDFLDDQV+FTGFSYG LSELPE +Y SCSMGSTIAGEIG+FGY
Sbjct: 499 VTELTLSNGMQVCYKSTDFLDDQVLFTGFSYGGLSELPESDYISCSMGSTIAGEIGMFGY 558

Query: 121 RPSVLMDMLAGKRAEVGTKLGAYMRTFSGDCSPSDLETALQLVYQLFTTNVTPGEEDVKI 180
           +PSVLMDMLA                        DLETALQLVYQLFTTNV P EE+V I
Sbjct: 559 KPSVLMDMLA------------------------DLETALQLVYQLFTTNVMPQEEEVGI 618

Query: 181 VMQMAEEAVRAQERDPYTAFAT--------------PIRLSDLRKVDPQKACEYFNNCFR 240
           VMQMAEE+VRA+ERDPYT FA               PIR+S+LRKVDP KACEYFN+CFR
Sbjct: 619 VMQMAEESVRARERDPYTVFANRVKELNYGNSYFFRPIRISELRKVDPLKACEYFNSCFR 678

Query: 241 DPSSFTVVIVGNINPSIALPLIQQYL-----PPEPVMDFNRDDLKGLPFTFPTSIVREVV 300
           DPS+FTVVIVGN++P+IALPLI QYL     PP+PV++FNRDDLKGLPFTFPT I +E V
Sbjct: 679 DPSTFTVVIVGNLDPTIALPLILQYLGGIPKPPQPVLNFNRDDLKGLPFTFPTKITKEFV 738

Query: 301 YSPMVEAQCSVQLCFPVELKNGTMVEEIHFVGFLSKLLETKMMQVLRFKHGQIYSAGVSV 360
            SPMVEAQCSVQLCFPV+L NGTM+EEIH +GFL KLLETK++Q LRF+HGQIYSA VSV
Sbjct: 739 RSPMVEAQCSVQLCFPVQLTNGTMIEEIHCIGFLGKLLETKIIQFLRFEHGQIYSAEVSV 798

Query: 361 FLGGNKPSRIGPVRGDISINFSCDPEISSKLVDLALDEILRLQEEGPTDQDVSTVLEIEQ 420
           FLGGNKPSR   +RGDIS+NFSCDPEISSKLVDLAL+EI+RLQ+EGP+ +D+S +LEIEQ
Sbjct: 799 FLGGNKPSRTADLRGDISVNFSCDPEISSKLVDLALEEIVRLQKEGPSQEDISAILEIEQ 858

Query: 421 RAHENGLQENYYWLDRILRSYQSRIYSGDVGTSFEIQDEGRLKVRNALTPSTAQLALRRI 480
           RAHENG+QENYYWLDRI+R YQSR+Y+GD+G S +I +EGRL++R +L P TAQ AL+RI
Sbjct: 859 RAHENGMQENYYWLDRIIRGYQSRVYAGDLGASCKILEEGRLRMRESLAPQTAQAALQRI 918

Query: 481 LPFPCTKQYTAVVLLPRSYQFRKLKSFSNS 492
           LP PC KQYTAV+L+P+  +F  L S  +S
Sbjct: 919 LPHPCKKQYTAVILMPQRSRFGFLSSIFSS 924

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890062.16.8e-26590.73zinc protease PQQL-like isoform X3 [Benincasa hispida][more]
XP_038890061.16.8e-26590.73zinc protease PQQL-like isoform X2 [Benincasa hispida][more]
XP_038890060.16.8e-26590.73zinc protease PQQL-like isoform X1 [Benincasa hispida][more]
XP_022999427.13.7e-26390.14zinc protease PQQL-like isoform X1 [Cucurbita maxima][more]
XP_022999428.13.7e-26390.14zinc protease PQQL-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FJT91.2e-20068.24Zinc protease PQQL-like OS=Arabidopsis thaliana OX=3702 GN=At5g56730 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KAU81.8e-26390.14zinc protease PQQL-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493804 P... [more]
A0A6J1KJP31.8e-26390.14zinc protease PQQL-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493804 P... [more]
A0A6J1DPE63.8e-26190.53zinc protease PQQL-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11102187... [more]
A0A6J1DM983.8e-26190.53zinc protease PQQL-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11102187... [more]
A0A1S3C9Q72.5e-26089.15zinc protease PQQL-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498391 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT5G56730.18.8e-20268.24Insulinase (Peptidase family M16) protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007863Peptidase M16, C-terminalPFAMPF05193Peptidase_M16_Ccoord: 212..395
e-value: 4.6E-14
score: 52.9
NoneNo IPR availableGENE3D3.30.830.10coord: 65..256
e-value: 2.9E-6
score: 29.1
NoneNo IPR availableGENE3D3.30.830.10coord: 309..481
e-value: 3.5E-10
score: 41.8
NoneNo IPR availablePANTHERPTHR43690NARDILYSINcoord: 2..492
NoneNo IPR availablePANTHERPTHR43690:SF20ZINC PROTEASE PQQL-LIKEcoord: 2..492
IPR011249Metalloenzyme, LuxS/M16 peptidase-likeSUPERFAMILY63411LuxS/MPP-like metallohydrolasecoord: 283..424
IPR011249Metalloenzyme, LuxS/M16 peptidase-likeSUPERFAMILY63411LuxS/MPP-like metallohydrolasecoord: 44..253

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr022307.1Sgr022307.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity