Sgr022088 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr022088 vs. NCBI nr
Match: XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 587/775 (75.74%), Postives = 650/775 (83.87%), Query Frame = 0

Query: 1    MEAFNLSSLLLLFFLFALLLTSTIAILHRLLGITFTWVGSFS-GRSPTRNRISL----QF 60
            MEAFNLSSLLLLFFLF LL TSTIA     +     ++GS S G +P+   + +     +
Sbjct: 815  MEAFNLSSLLLLFFLFPLLQTSTIATQKSYI----VYLGSHSHGSNPSSVDLQIATESHY 874

Query: 61   TRVVARKYSNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTT 120
            + + +   SNEAAKEAIFYSYNRHINGFAAMLD KVAEDLA++PAV SVHEN+ R+LHTT
Sbjct: 875  SLLGSLLGSNEAAKEAIFYSYNRHINGFAAMLDHKVAEDLARNPAVASVHENKGRKLHTT 934

Query: 121  SSWNFLGVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGS 180
            SSW FLGVEHD G P+ SIWNLASFGESTIIANLDTGVWPESKSFSDEGYGP+P+RW+GS
Sbjct: 935  SSWKFLGVEHDDGIPTKSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPVPTRWKGS 994

Query: 181  CEGGSKFHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGAS 240
            CEGGSKFHCNRKLIGARYFNKGYAAYVG L+ +YETARDH+GHGTHTLSTAGGNFISGA+
Sbjct: 995  CEGGSKFHCNRKLIGARYFNKGYAAYVGSLNATYETARDHDGHGTHTLSTAGGNFISGAN 1054

Query: 241  VFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGG 300
            VFGNGNGTAKGGSP+A V AYKVCWP +   G CFDAD LAAIEAAISDGVDVLS+SLGG
Sbjct: 1055 VFGNGNGTAKGGSPKALVAAYKVCWPPVDSGGSCFDADILAAIEAAISDGVDVLSLSLGG 1114

Query: 301  GSKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSY 360
             SKDFSDDV AIGAFHAVQ GI+VVCSAGNSGP+  TVENVAPW+ TVGASTINR FTSY
Sbjct: 1115 DSKDFSDDVAAIGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIFTVGASTINREFTSY 1174

Query: 361  VALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKI 420
            VALGNKKHIKGASLSDKILPAQKFYPLISAA AKAN+VS DDAQ C EGSLDP KVKGKI
Sbjct: 1175 VALGNKKHIKGASLSDKILPAQKFYPLISAAYAKANHVSFDDAQLCEEGSLDPRKVKGKI 1234

Query: 421  IVCLRGDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRTDR 480
            I+CLRG++ RVDKGYVAAQAGAVGMILAN+E+N D LIADAHLLP SH S         +
Sbjct: 1235 IICLRGENARVDKGYVAAQAGAVGMILANTEQNEDELIADAHLLPVSHVSY-TDGQSIYQ 1294

Query: 481  ARNQTSTNYGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSE 540
              N T T   ++   R++   +      +  ++    +    L+PDITAPGVNI+AAYSE
Sbjct: 1295 YINSTKTPMAYMTHARTELGIKPAPVMASFSSRGPNTMEESILKPDITAPGVNILAAYSE 1354

Query: 541  DAPPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRA 600
            DA PSGSS+DNRRIPFN+VSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRA
Sbjct: 1355 DASPSGSSFDNRRIPFNIVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRA 1414

Query: 601  NNFHPILNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKF 660
            N+ HPILNT ++KANP AYGAGHV+PN A+NPGLVYDLT  DYLNFLC  GYN TQI KF
Sbjct: 1415 NDKHPILNTEELKANPFAYGAGHVQPNRAMNPGLVYDLTTKDYLNFLCVLGYNKTQISKF 1474

Query: 661  SNTSFICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVSI 720
            SNTSF+CSKSFKLTDFNYPSISIPN+KPG V IKRRVKNVG P TYVARVKVP G SVS+
Sbjct: 1475 SNTSFVCSKSFKLTDFNYPSISIPNMKPGLVTIKRRVKNVGKPSTYVARVKVPPGASVSV 1534

Query: 721  EPSTLKFTGIDEEKTFKVVLQRIANNKH-RGYVFGSLAWSDGNHHVRSPIAVNLG 765
            +PSTLKFTGIDEEK+FKV++  +A+NKH RGYVFGSL W DG HHVRSPI VNLG
Sbjct: 1535 KPSTLKFTGIDEEKSFKVIVGSVADNKHSRGYVFGSLVWEDGKHHVRSPIVVNLG 1584

BLAST of Sgr022088 vs. NCBI nr
Match: XP_016900594.1 (PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo])

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 547/706 (77.48%), Postives = 607/706 (85.98%), Query Frame = 0

Query: 64  SNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGV 123
           SNEAAKEAIFYSYNRHINGFAA+LD KV EDLA++PAV S+HEN+ R+LHTTSSW FLGV
Sbjct: 43  SNEAAKEAIFYSYNRHINGFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGV 102

Query: 124 EHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSKFH 183
           EHD G P NSIWN ASFGESTII NLDTGVWPESKSF+DEGYGP+P+RW+GSCEGGSKFH
Sbjct: 103 EHDDGIPHNSIWNRASFGESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFH 162

Query: 184 CNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGASVFGNGNGT 243
           CNRKLIGARYFNKGYAAY G L+ SYETARD+EGHGTHTLSTAGGNFISGA+VFGNGNGT
Sbjct: 163 CNRKLIGARYFNKGYAAYAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGT 222

Query: 244 AKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGGGSKDFSDD 303
           AKGGSP+A V AYKVCWPQ+   GGCFDAD LAAIEAAISDGVD+LS+SLGGG+KDFS+D
Sbjct: 223 AKGGSPKALVAAYKVCWPQVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSED 282

Query: 304 VTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVALGNKKH 363
           VTAIGAFHAVQ GIIVVCSAGNSGP+  T+EN APW++TVGASTINR FTSYVALGNKKH
Sbjct: 283 VTAIGAFHAVQQGIIVVCSAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKH 342

Query: 364 IKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKIIVCLRGDS 423
           IKGASLSDKILP QKFYPLI+AADAKANNVS D AQ C  GSLDP+KVKGKII+CLRG++
Sbjct: 343 IKGASLSDKILPEQKFYPLINAADAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGEN 402

Query: 424 GRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRTDRARNQTSTN 483
            RVDKGY AAQAGAVGMILAN+E+NGD LIADAHLLP SH S         +  N T T 
Sbjct: 403 ARVDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSY-TDGQSIYQYINFTKTP 462

Query: 484 YGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSEDAPPSGSS 543
             ++   R++   +      +  ++    +    L+PDITAPGVNI+AAYSEDA PSGS 
Sbjct: 463 MAYMTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSL 522

Query: 544 YDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPILN 603
           +DNRRIPFN+VSGTSMSCPHISGIVGLLKTLYP WSPAAIKSAIMTTAETRAN+ HPILN
Sbjct: 523 FDNRRIPFNIVSGTSMSCPHISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILN 582

Query: 604 TTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKFSNTSFICS 663
           T  +KANP AYGAGHV+PN A+NPGLVYDLT  DY+NFLCA+GYN +QI KFS TSF+CS
Sbjct: 583 TVNLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCS 642

Query: 664 KSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVSIEPSTLKFT 723
           KSFKLTDFNYPSISIP++K G V I RRVKNVG P TYVARVKVP+G+SVS+EP TLKFT
Sbjct: 643 KSFKLTDFNYPSISIPDMKSGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFT 702

Query: 724 GIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNLG 765
           GIDEEK+FKVV+  +ANNKHRGYVFGSL W DG HHVRSPI VNLG
Sbjct: 703 GIDEEKSFKVVIGSVANNKHRGYVFGSLIWEDGKHHVRSPIVVNLG 747

BLAST of Sgr022088 vs. NCBI nr
Match: XP_022143461.1 (subtilisin-like protease SBT5.4 [Momordica charantia])

HSP 1 Score: 1072.8 bits (2773), Expect = 1.2e-309
Identity = 555/778 (71.34%), Postives = 638/778 (82.01%), Query Frame = 0

Query: 1   MEAFNLSSLLLLFFLFALLLTSTIAILHRLLGITFTWVGSFS-GRSPTRNRISL----QF 60
           ME FN SSLLLLFFLFA+L TST A     +     ++GS S G +P+   + L     +
Sbjct: 1   MEGFNHSSLLLLFFLFAVLQTSTFATKKSYI----FYLGSHSHGLNPSAIDLQLATESHY 60

Query: 61  TRVVARKYSNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTT 120
             + +   SN AAK+AIFYSYN++INGFAA+LD KVA++LAKHP+VVSVHEN+ R+LHTT
Sbjct: 61  NLLGSLLGSNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTT 120

Query: 121 SSWNFLGVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGS 180
            SW+FLG+E+DG  PSNSIWNLASFGESTII NLDTGVWPESKSFSD+GYGPIPSRWRGS
Sbjct: 121 GSWSFLGLENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGS 180

Query: 181 CEGGSKFHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGAS 240
           CEGGSKF CNRKLIGARYFNKGY A +GPL+ SYETARD EGHGTHTLSTAGGNF+SGAS
Sbjct: 181 CEGGSKFRCNRKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGAS 240

Query: 241 VFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGG 300
           VFGNGNGTAKGGSPRARV AY+VCWP +L +GGCF AD LA  EAAI DGVDVLS+SLGG
Sbjct: 241 VFGNGNGTAKGGSPRARVAAYRVCWPAVL-TGGCFMADILAGFEAAIHDGVDVLSVSLGG 300

Query: 301 GSKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSY 360
             ++FSDD  AIGAFHAVQHGI VVCSAGNSGP   TV NVAPW++TVGAST +RLF SY
Sbjct: 301 SPEEFSDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASY 360

Query: 361 VALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKI 420
           V LGN+KHIKGASLSDKILPAQKFYPLIS ADAKA+NVS++ AQ C EGSLDP+KV+GKI
Sbjct: 361 VGLGNRKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKI 420

Query: 421 IVCLRGDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNG-------- 480
           +VCLRGD+ RVDKGYVAAQAGAVGMILAN+++NGD L+ADAHLLPASH S          
Sbjct: 421 VVCLRGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQY 480

Query: 481 LHDSRTDRA-RNQTSTNYGFILIQRSQYNRRVNTQAQNLEIVSNFFLQPDITAPGVNIIA 540
           +  ++   A      T  G   ++ + +    +++  N   +    L+PDI APGV+IIA
Sbjct: 481 IKSTKIPMAYMTHVKTELG---VKPAPFMASFSSRGPN--TIEESILKPDIIAPGVSIIA 540

Query: 541 AYSEDAPPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTA 600
           AYSE+A PSGSS+D RR PFN  SGTSMSCPH+SGIVGLLKT YPKWSPAAIKSA+MTTA
Sbjct: 541 AYSEEASPSGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTA 600

Query: 601 ETRANNFHPILNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQ 660
            T+AN+ HPILNTTQ+KANPL+YGAGHVRPN A+NPGLVYDLT+ DYLNFLCARGYN TQ
Sbjct: 601 ATKANDLHPILNTTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQ 660

Query: 661 IKKFSNTSFICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGI 720
           IKKFS+  F CS SFKLTDFNYPSISIPNLK GPV  KRRVKNVGSPGTYVA+VK P G+
Sbjct: 661 IKKFSSAPFACSHSFKLTDFNYPSISIPNLKFGPVKAKRRVKNVGSPGTYVAQVKAPPGV 720

Query: 721 SVSIEPSTLKFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNLG 765
           +VS+EP+ LKFTGI EE++F+VV++R+ N + RGYVFG LAWSDGNH VRSPIAVNLG
Sbjct: 721 AVSVEPNMLKFTGIGEEQSFRVVVRRVENEERRGYVFGWLAWSDGNHRVRSPIAVNLG 768

BLAST of Sgr022088 vs. NCBI nr
Match: XP_022932414.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 1063.1 bits (2748), Expect = 1.1e-306
Identity = 554/775 (71.48%), Postives = 625/775 (80.65%), Query Frame = 0

Query: 1   MEAFNLSSLLLLFFLFALLLTSTIAILHRLLGITFTWVGSFS-GRSPT----RNRISLQF 60
           ME FNLSSL+LLFFLF L  TSTIA     +     ++GS S G +P+    R      +
Sbjct: 1   MEGFNLSSLILLFFLFPLFQTSTIATQKSYI----VYLGSHSHGSNPSSVDLRIATESHY 60

Query: 61  TRVVARKYSNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTT 120
           + + +   SNE AKEAIFYSY+RHINGFAA+LD KVA+DL +HPAVVSVHEN+ R+LHTT
Sbjct: 61  SLLGSLLGSNEIAKEAIFYSYHRHINGFAAVLDHKVAQDLTRHPAVVSVHENKMRKLHTT 120

Query: 121 SSWNFLGVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGS 180
           SSW FL +E+  G+P NSIWN A+FGESTIIANLDTGVWPESKSF+DEGYG IPSRWRGS
Sbjct: 121 SSWEFLDLENGEGTPPNSIWNGANFGESTIIANLDTGVWPESKSFNDEGYGAIPSRWRGS 180

Query: 181 CEGGSKFHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGAS 240
           CEGGS FHCNRKLIGARYFNKGY A  G LD S++TARDH+GHGTHTLSTAGGNF+SGA+
Sbjct: 181 CEGGSNFHCNRKLIGARYFNKGYTALAGSLDDSFDTARDHDGHGTHTLSTAGGNFVSGAN 240

Query: 241 VFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGG 300
           VFGNGNGTAKGGSP+A V AYKVCWP   + G C DAD LAAIEAAI+DGVDVLS+SLG 
Sbjct: 241 VFGNGNGTAKGGSPKAFVAAYKVCWP-TFHGGQCSDADILAAIEAAITDGVDVLSLSLGR 300

Query: 301 GSKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSY 360
           GS +F DDVTAIG+FHAVQ GI+VVCS GNSGP   +VENVAPW+ TV ASTI R FTSY
Sbjct: 301 GSTEFFDDVTAIGSFHAVQQGIVVVCSGGNSGPDPQSVENVAPWLFTVAASTITRQFTSY 360

Query: 361 VALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKI 420
           VALGNKKHI GAS+SDKILPAQ+FYPLI++ DAKA N+SV+ A+ CVEGSLDP KVKGKI
Sbjct: 361 VALGNKKHITGASISDKILPAQQFYPLITSVDAKAINISVETAKLCVEGSLDPRKVKGKI 420

Query: 421 IVCLR-GDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRTD 480
           IVC++ GDS RVDKGYVAAQAGAVGMILANSEE+G+ LIADAHLLP SH S  +      
Sbjct: 421 IVCVKGGDSARVDKGYVAAQAGAVGMILANSEEDGNELIADAHLLPVSHISY-IDGEIVY 480

Query: 481 RARNQTSTNYGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYS 540
              N T T   ++   R++   +      +  ++    +    L+PDITAPGVNIIAAYS
Sbjct: 481 EYINSTKTPVAYMTQVRTETGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYS 540

Query: 541 EDAPPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETR 600
           EDA PSGS +DNRRIPFNVVSGTSMSCPHISGIV LLKT+YPKWSPAAIKSAIMTTAETR
Sbjct: 541 EDASPSGSPFDNRRIPFNVVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETR 600

Query: 601 ANNFHPILNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKK 660
           AN+ HPIL++T++ ANPLAYGAGHV PN A NPGLVYDLT  DYLNFLCARGYN TQ+ K
Sbjct: 601 ANDLHPILDSTKLVANPLAYGAGHVHPNRAANPGLVYDLTTNDYLNFLCARGYNKTQLSK 660

Query: 661 FSNTSFICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVS 720
           FSNTSF+CSKSFKLTDFNYPSISIP +K  PV IKR VKNVGSP TYVARVKVP G+ VS
Sbjct: 661 FSNTSFVCSKSFKLTDFNYPSISIPKMKSEPVTIKRTVKNVGSPSTYVARVKVPPGVLVS 720

Query: 721 IEPSTLKFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNLG 765
           +EPSTLKFT  DEEKTFKVV + + N KHRGYVFGSL W DG HHVRS I VNLG
Sbjct: 721 VEPSTLKFTRTDEEKTFKVVFRSVTNTKHRGYVFGSLKWLDGKHHVRSSIVVNLG 769

BLAST of Sgr022088 vs. NCBI nr
Match: XP_023553316.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1060.1 bits (2740), Expect = 9.3e-306
Identity = 551/775 (71.10%), Postives = 623/775 (80.39%), Query Frame = 0

Query: 1   MEAFNLSSLLLLFFLFALLLTSTIAILHRLLGITFTWVGSFS-GRSPT----RNRISLQF 60
           ME FNLSS +LLFFLF L  TSTIA     +     ++GS S G +P+    R      +
Sbjct: 1   MEGFNLSSFILLFFLFPLFQTSTIATQKSYI----VYLGSHSHGSNPSSVDLRIATESHY 60

Query: 61  TRVVARKYSNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTT 120
           + + +   SNE AKEAIFYSYNRHINGFAA+LD KVA DL +HPAV+SVHEN+ R+LHTT
Sbjct: 61  SLLGSLLGSNEIAKEAIFYSYNRHINGFAAVLDHKVARDLTRHPAVLSVHENKMRKLHTT 120

Query: 121 SSWNFLGVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGS 180
           SSW FL +E+  G+P NSIWN A+FGEST+I NLDTGVWPESKSFSDEGYG IPSRWRGS
Sbjct: 121 SSWEFLELENGEGTPPNSIWNGANFGESTVIGNLDTGVWPESKSFSDEGYGAIPSRWRGS 180

Query: 181 CEGGSKFHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGAS 240
           CEGGS FHCN+KLIGARYFNKGY A  G LD S++TARDH+GHGTHTLSTAGGNF+SGA+
Sbjct: 181 CEGGSNFHCNKKLIGARYFNKGYTALAGSLDGSFDTARDHDGHGTHTLSTAGGNFVSGAN 240

Query: 241 VFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGG 300
           VFGNGNGTAKGGSP+A V AYKVCWP   + G C DAD LAAIEAAI+DGVDVLS+SLG 
Sbjct: 241 VFGNGNGTAKGGSPKAFVAAYKVCWP-TFHGGQCSDADILAAIEAAITDGVDVLSLSLGR 300

Query: 301 GSKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSY 360
           GS +F DDVTAIG+FHAVQ GI+VVCS GNSGP   +VENVAPW+ TV ASTI R FTSY
Sbjct: 301 GSTEFFDDVTAIGSFHAVQQGIVVVCSGGNSGPDPQSVENVAPWLFTVAASTITRQFTSY 360

Query: 361 VALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKI 420
           VALGNKKHI GAS+SDKILPAQ+FYPLI++ DAKA N+SV+ A+ CVEGSLDP KVKGKI
Sbjct: 361 VALGNKKHITGASISDKILPAQQFYPLITSEDAKAINISVETAKLCVEGSLDPRKVKGKI 420

Query: 421 IVCLR-GDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRTD 480
           IVC+R GDS RVDKGYVAAQAGAVGMILAN+EE+G+ LIADAHLLP SH S  +      
Sbjct: 421 IVCVRGGDSARVDKGYVAAQAGAVGMILANNEEDGNELIADAHLLPVSHISY-IDGETVY 480

Query: 481 RARNQTSTNYGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYS 540
              N T T   ++   R++   +      +  ++    +    L+PDITAPGVNIIAAYS
Sbjct: 481 EYINSTKTPVAYMTHVRTETGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYS 540

Query: 541 EDAPPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETR 600
           EDA PSGS +DNRRIPFNVVSGTSMSCPHISGIVGLLKT+YPKWSPAAIKSAIMTTAETR
Sbjct: 541 EDASPSGSPFDNRRIPFNVVSGTSMSCPHISGIVGLLKTIYPKWSPAAIKSAIMTTAETR 600

Query: 601 ANNFHPILNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKK 660
           AN+ HPIL++T++ ANPLAYGAGHV PN A NPGLVYDLT  DYLNFLCARGYN  Q+ K
Sbjct: 601 ANDLHPILDSTKLVANPLAYGAGHVHPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSK 660

Query: 661 FSNTSFICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVS 720
           FSNTSF+C KSFKLTDFNYPSISI N+K GPV IKR VKNVGSP TYV RVKVP G+ VS
Sbjct: 661 FSNTSFVCLKSFKLTDFNYPSISISNMKSGPVTIKRTVKNVGSPSTYVVRVKVPPGVLVS 720

Query: 721 IEPSTLKFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNLG 765
           +EPSTLKF+  DEEKTFKVV + +ANNKHRGYVFGSL W DG HHVRS I VNLG
Sbjct: 721 VEPSTLKFSRTDEEKTFKVVFRSLANNKHRGYVFGSLKWLDGKHHVRSSIVVNLG 769

BLAST of Sgr022088 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 2.6e-226
Identity = 404/709 (56.98%), Postives = 502/709 (70.80%), Query Frame = 0

Query: 64  SNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGV 123
           S E A +AIFYSY +HINGFAA LD  +A +++KHP VVSV  N+  +LHTT SW+FLG+
Sbjct: 67  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126

Query: 124 EHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEG--GSK 183
           EH+   PS+SIW  A FGE TIIANLDTGVWPESKSF DEG GPIPSRW+G C+    + 
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 186

Query: 184 FHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGASVFGNGN 243
           FHCNRKLIGARYFNKGYAA VG L++S+++ RD +GHG+HTLSTA G+F+ G S+FG GN
Sbjct: 187 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGN 246

Query: 244 GTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGGGSKDFS 303
           GTAKGGSPRARV AYKVCWP  +    C+DAD LAA +AAI DG DV+S+SLGG    F 
Sbjct: 247 GTAKGGSPRARVAAYKVCWPP-VKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFF 306

Query: 304 DDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVALGNK 363
           +D  AIG+FHA +  I+VVCSAGNSGP+ +TV NVAPW +TVGAST++R F S + LGN 
Sbjct: 307 NDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNG 366

Query: 364 KHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKIIVCLRG 423
           KH KG SLS   LP  KFYP++++ +AKA N S  DAQ C  GSLDP K KGKI+VCLRG
Sbjct: 367 KHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRG 426

Query: 424 DSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDS-RTDRARNQT 483
            +GRV+KG   A  G +GM+L N+   G+ L+AD H+LPA+  ++   DS    R  +QT
Sbjct: 427 QNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTS--KDSFAVSRYISQT 486

Query: 484 STNYGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSEDAPPS 543
                 I   R+    +      +  ++   IV+   L+PDITAPGV++IAAY+    P+
Sbjct: 487 KKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPT 546

Query: 544 GSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHP 603
              +D RR+ FN +SGTSMSCPHISGI GLLKT YP WSPAAI+SAIMTTA    +   P
Sbjct: 547 NEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGP 606

Query: 604 ILNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKFSNTSF 663
           I N T +KA P ++GAGHV+PN AVNPGLVYDL I DYLNFLC+ GYN +QI  FS  +F
Sbjct: 607 IQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNF 666

Query: 664 ICSK-SFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVSIEPST 723
            CS     L + NYPSI++PNL    V + R VKNVG P  Y  +V  P+G+ V+++P++
Sbjct: 667 TCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTS 726

Query: 724 LKFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNL 764
           L FT + E+KTFKV+L +   N  +GYVFG L WSD  H VRSPI V L
Sbjct: 727 LNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Sgr022088 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 2.1e-223
Identity = 408/708 (57.63%), Postives = 505/708 (71.33%), Query Frame = 0

Query: 64  SNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGV 123
           S+E AKEAIFYSY RHINGFAA+LD   A ++AKHP VVSV  N+ R+LHTT SWNF+ +
Sbjct: 77  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 136

Query: 124 EHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSKFH 183
             +G    +S+WN A +GE TIIANLDTGVWPESKSFSDEGYG +P+RW+G C       
Sbjct: 137 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVP 196

Query: 184 CNRKLIGARYFNKGYAAYVG-PLDTSYETARDHEGHGTHTLSTAGGNFISGASVFGNGNG 243
           CNRKLIGARYFNKGY AY G P + SYET RDH+GHG+HTLSTA GNF+ GA+VFG GNG
Sbjct: 197 CNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNG 256

Query: 244 TAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGGGSKDFSD 303
           TA GGSP+ARV AYKVCWP  +    CFDAD LAAIEAAI DGVDVLS S+GG + D+  
Sbjct: 257 TASGGSPKARVAAYKVCWPP-VDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMS 316

Query: 304 DVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVALGNKK 363
           D  AIG+FHAV++G+ VVCSAGNSGP   TV NVAPWV+TVGAS+++R F ++V L N +
Sbjct: 317 DGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQ 376

Query: 364 HIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKIIVCLRGD 423
             KG SLS K LP +K Y LISAADA   N +V DA  C +GSLDP+KVKGKI+VCLRGD
Sbjct: 377 SFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGD 436

Query: 424 SGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRT-DRARNQTS 483
           + RVDKG  AA AGA GM+L N + +G+ +I+DAH+LPAS       D  T     + T 
Sbjct: 437 NARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQID--YKDGETLFSYLSSTK 496

Query: 484 TNYGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSEDAPPSG 543
              G+I    +  N +      +  ++    ++   L+PDITAPGVNIIAA++E   P+ 
Sbjct: 497 DPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTD 556

Query: 544 SSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPI 603
              DNRR PFN  SGTSMSCPHISG+VGLLKTL+P WSPAAI+SAIMTT+ TR N   P+
Sbjct: 557 LDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPM 616

Query: 604 LNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKFS-NTSF 663
           ++ +  KANP +YG+GHV+PN A +PGLVYDLT  DYL+FLCA GYN T ++ F+ +  +
Sbjct: 617 VDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQY 676

Query: 664 ICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVSIEPSTL 723
            C +   L DFNYPSI++PNL  G + + R++KNVG P TY AR + P G+ VS+EP  L
Sbjct: 677 TCRQGANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQL 736

Query: 724 KFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNL 764
            F    E K F++ L+ +      GYVFG L W+D +H+VRSPI V L
Sbjct: 737 TFNKTGEVKIFQMTLRPLPVTP-SGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Sgr022088 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 650.2 bits (1676), Expect = 2.9e-185
Identity = 390/791 (49.30%), Postives = 489/791 (61.82%), Query Frame = 0

Query: 4   FNLSSLLLLFFLFALLLTSTIAILHRLLGITFTWVGSFS-GRSPTRNRISL----QFTRV 63
           F L  +L  FFLF  LL    A ++        ++G+ S G SPT   + L     +  +
Sbjct: 6   FCLHLILSSFFLFTFLL----AAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLL 65

Query: 64  VARKYSNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSW 123
            +   S E AKEAI YSYNRHINGFAA+L+ + A D+AK+P VVSV  ++E +LHTT SW
Sbjct: 66  GSIFGSREKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSW 125

Query: 124 NFLGVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGS-CE 183
            FLG+   G    NS W    FGE+TII N+DTGVWPES+SFSD+GYG +PS+WRG  C+
Sbjct: 126 EFLGLHRRG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQ 185

Query: 184 -----GGSKFHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFIS 243
                G  K  CNRKLIGARY+NK + A+ G LD    TARD  GHGTHTLSTAGGNF+ 
Sbjct: 186 INKLPGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVP 245

Query: 244 GASVFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMS 303
           GA VF  GNGTAKGGSPRARV AYKVCW  L     C+ AD LAAI+ AI DGVDV+++S
Sbjct: 246 GARVFAVGNGTAKGGSPRARVAAYKVCW-SLTDPASCYGADVLAAIDQAIDDGVDVINVS 305

Query: 304 LGGG----SKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTI 363
            G      ++    D  +IGAFHA+   I++V SAGN GP+  TV NVAPWV T+ AST+
Sbjct: 306 FGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTL 365

Query: 364 NRLFTSYVALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDP 423
           +R F+S + + N+  I+GASL   + P Q F  LI + DAK  N +  DAQ C  G+LD 
Sbjct: 366 DRDFSSNLTINNQL-IEGASLFVNLPPNQAF-SLILSTDAKLANATFRDAQLCRRGTLDR 425

Query: 424 EKVKGKIIVCLR-GDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLL-----PAS 483
            KV GKI++C R G    V +G  A  AGA GMIL N  +NG +L A+ H+      P  
Sbjct: 426 TKVNGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPR 485

Query: 484 HNSNGLHDSRTD---------RARNQTSTNYGFILIQRSQYNRRVNTQAQNLEIVSNFFL 543
              +  H  +T          +  +    +    L  R       +  ++    +    L
Sbjct: 486 RAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSIL 545

Query: 544 QPDITAPGVNIIAAYSEDAPPSGSSYDNRR-IPFNVVSGTSMSCPHISGIVGLLKTLYPK 603
           +PD+TAPGVNI+AAYSE A  S    DNRR   FNV+ GTSMSCPH SGI GLLKT +P 
Sbjct: 546 KPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPS 605

Query: 604 WSPAAIKSAIMTTAETRANNFHPILNT-TQVKANPLAYGAGHVRPNGAVNPGLVYDLTIT 663
           WSPAAIKSAIMTTA T  N   PI +   +  A+  AYG+GHVRP+ A+ PGLVYDL++T
Sbjct: 606 WSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLT 665

Query: 664 DYLNFLCARGYNGTQIKKFS-NTSFICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNV 723
           DYLNFLCA GY+   I   + N +FICS S  + D NYPSI++PNL+  PV I R V NV
Sbjct: 666 DYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNV 725

Query: 724 GSPGTYVARVKVPRGISVSIEPSTLKFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSD 762
           G P TY    + P G S+++ P +L FT I E KTFKV++Q  +    R Y FG L W+D
Sbjct: 726 GPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTD 785

BLAST of Sgr022088 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 4.5e-162
Identity = 320/706 (45.33%), Postives = 439/706 (62.18%), Query Frame = 0

Query: 72  IFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGVEHDGGSPS 131
           + Y+Y   I+GF+  L  + A+ L   P V+SV      +LHTT +  FLG++      +
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 132 NSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSKFH---CNRKL 191
             ++  A      ++  LDTGVWPESKS+SDEG+GPIPS W+G CE G+ F    CNRKL
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 192 IGARYFNKGYAAYVGPLDTSYE--TARDHEGHGTHTLSTAGGNFISGASVFGNGNGTAKG 251
           IGAR+F +GY + +GP+D S E  + RD +GHGTHT STA G+ + GAS+ G  +GTA+G
Sbjct: 185 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 252 GSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGGGSKDFSDDVTA 311
            +PRARV  YKVCW      GGCF +D LAAI+ AI+D V+VLSMSLGGG  D+  D  A
Sbjct: 245 MAPRARVAVYKVCW-----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 304

Query: 312 IGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVALGNKKHIKG 371
           IGAF A++ GI+V CSAGN+GPS +++ NVAPW+ TVGA T++R F +   LGN K+  G
Sbjct: 305 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 364

Query: 372 ASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKIIVCLRGDSGRV 431
            SL        K  P I A +A     +  +   C+ G+L PEKVKGKI++C RG + RV
Sbjct: 365 VSLFKGEALPDKLLPFIYAGNAS----NATNGNLCMTGTLIPEKVKGKIVMCDRGINARV 424

Query: 432 DKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGL-----HDSRTDRARNQTS 491
            KG V   AG VGMILAN+  NG+ L+ADAHLLPA+           H   TD     + 
Sbjct: 425 QKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASI 484

Query: 492 TNYGFIL-IQRSQYNRRVNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSEDAPPSGSSYD 551
           +  G ++ ++ S      +++  N  I  N  L+PD+ APGVNI+AA++  A P+G + D
Sbjct: 485 SILGTVVGVKPSPVVAAFSSRGPN-SITPN-ILKPDLIAPGVNILAAWTGAAGPTGLASD 544

Query: 552 NRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPILN-T 611
           +RR+ FN++SGTSMSCPH+SG+  LLK+++P+WSPAAI+SA+MTTA     +  P+L+  
Sbjct: 545 SRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA 604

Query: 612 TQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKFSNTSFIC-- 671
           T   + P  +GAGHV P  A NPGL+YDLT  DYL FLCA  Y   QI+  S  ++ C  
Sbjct: 605 TGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDP 664

Query: 672 SKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARV-KVPRGISVSIEPSTLK 731
           SKS+ + D NYPS ++     G     R V +VG  GTY  +V     G+ +S+EP+ L 
Sbjct: 665 SKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 724

Query: 732 FTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVN 763
           F   +E+K++ V     ++       FGS+ WSDG H V SP+A++
Sbjct: 725 FKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Sgr022088 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.5e-144
Identity = 301/725 (41.52%), Postives = 438/725 (60.41%), Query Frame = 0

Query: 63  YSNEAAKEA-IFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFL 122
           YS E A+E+ I + Y+   +GF+A++    A++L  HPAV++V E+R R+LHTT S  FL
Sbjct: 48  YSTEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFL 107

Query: 123 GVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSK 182
           G+++  G     +W+ + +G   II   DTG+WPE +SFSD   GPIP RWRG CE G++
Sbjct: 108 GLQNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGAR 167

Query: 183 F---HCNRKLIGARYFNKG-YAAYVGPLDTSYE--TARDHEGHGTHTLSTAGGNFISGAS 242
           F   +CNRK+IGAR+F KG  AA +G ++ + E  + RD +GHGTHT STA G     AS
Sbjct: 168 FSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKAS 227

Query: 243 VFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGG 302
           + G  +G AKG +P+AR+ AYKVCW       GC D+D LAA +AA+ DGVDV+S+S+GG
Sbjct: 228 MSGYASGVAKGVAPKARIAAYKVCWK----DSGCLDSDILAAFDAAVRDGVDVISISIGG 287

Query: 303 G---SKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLF 362
           G   +  +  D  AIG++ A   GI V  SAGN GP+  +V N+APWV TVGASTI+R F
Sbjct: 288 GDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNF 347

Query: 363 TSYVALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVK 422
            +   LG+   ++G SL   +    + +P++    +  ++ S+     C+E +LDP++V+
Sbjct: 348 PADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSASL-----CMENTLDPKQVR 407

Query: 423 GKIIVCLRGDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPA----SHNSNGL 482
           GKI++C RG S RV KG V  +AG VGMILAN   NG+ L+ DAHL+PA    S+  + +
Sbjct: 408 GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRI 467

Query: 483 HDSRTDRARNQTSTNYGFILIQRSQYNRRVNTQAQNLEIVSNFFLQPDITAPGVNIIAAY 542
               +       S ++   ++         +   +    +S   L+PD+ APGVNI+AA+
Sbjct: 468 KAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAW 527

Query: 543 SEDAPPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAET 602
           ++   P+G   D R+  FN++SGTSM+CPH+SG   LLK+ +P WSPA I+SA+MTT   
Sbjct: 528 TDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNL 587

Query: 603 RANNFHPILN-TTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQI 662
             N+   +++ +T   A P  YG+GH+    A+NPGLVYD+T  DY+ FLC+ GY    I
Sbjct: 588 VDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTI 647

Query: 663 KKFSNTSFICSKSFKLT--DFNYPSIS--IPNLKPGPV--IIKRRVKNVG-SPGTYVARV 722
           +  + T   C  + K +  + NYPSI+   P  + G V   + R   NVG +   Y AR+
Sbjct: 648 QVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARI 707

Query: 723 KVPRGISVSIEPSTLKFTGIDEEKTFKVVLQRIANN---KHRGYVFGSLAWSDGNHH-VR 762
           + PRG++V+++P  L FT   + +++ V +     N      G VFGS+ W DG  H VR
Sbjct: 708 ESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVR 758

BLAST of Sgr022088 vs. ExPASy TrEMBL
Match: A0A1S4DX85 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=1)

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 547/706 (77.48%), Postives = 607/706 (85.98%), Query Frame = 0

Query: 64  SNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGV 123
           SNEAAKEAIFYSYNRHINGFAA+LD KV EDLA++PAV S+HEN+ R+LHTTSSW FLGV
Sbjct: 43  SNEAAKEAIFYSYNRHINGFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGV 102

Query: 124 EHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSKFH 183
           EHD G P NSIWN ASFGESTII NLDTGVWPESKSF+DEGYGP+P+RW+GSCEGGSKFH
Sbjct: 103 EHDDGIPHNSIWNRASFGESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFH 162

Query: 184 CNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGASVFGNGNGT 243
           CNRKLIGARYFNKGYAAY G L+ SYETARD+EGHGTHTLSTAGGNFISGA+VFGNGNGT
Sbjct: 163 CNRKLIGARYFNKGYAAYAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGT 222

Query: 244 AKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGGGSKDFSDD 303
           AKGGSP+A V AYKVCWPQ+   GGCFDAD LAAIEAAISDGVD+LS+SLGGG+KDFS+D
Sbjct: 223 AKGGSPKALVAAYKVCWPQVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSED 282

Query: 304 VTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVALGNKKH 363
           VTAIGAFHAVQ GIIVVCSAGNSGP+  T+EN APW++TVGASTINR FTSYVALGNKKH
Sbjct: 283 VTAIGAFHAVQQGIIVVCSAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKH 342

Query: 364 IKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKIIVCLRGDS 423
           IKGASLSDKILP QKFYPLI+AADAKANNVS D AQ C  GSLDP+KVKGKII+CLRG++
Sbjct: 343 IKGASLSDKILPEQKFYPLINAADAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGEN 402

Query: 424 GRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRTDRARNQTSTN 483
            RVDKGY AAQAGAVGMILAN+E+NGD LIADAHLLP SH S         +  N T T 
Sbjct: 403 ARVDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSY-TDGQSIYQYINFTKTP 462

Query: 484 YGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSEDAPPSGSS 543
             ++   R++   +      +  ++    +    L+PDITAPGVNI+AAYSEDA PSGS 
Sbjct: 463 MAYMTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSL 522

Query: 544 YDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPILN 603
           +DNRRIPFN+VSGTSMSCPHISGIVGLLKTLYP WSPAAIKSAIMTTAETRAN+ HPILN
Sbjct: 523 FDNRRIPFNIVSGTSMSCPHISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILN 582

Query: 604 TTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKFSNTSFICS 663
           T  +KANP AYGAGHV+PN A+NPGLVYDLT  DY+NFLCA+GYN +QI KFS TSF+CS
Sbjct: 583 TVNLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCS 642

Query: 664 KSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVSIEPSTLKFT 723
           KSFKLTDFNYPSISIP++K G V I RRVKNVG P TYVARVKVP+G+SVS+EP TLKFT
Sbjct: 643 KSFKLTDFNYPSISIPDMKSGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFT 702

Query: 724 GIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNLG 765
           GIDEEK+FKVV+  +ANNKHRGYVFGSL W DG HHVRSPI VNLG
Sbjct: 703 GIDEEKSFKVVIGSVANNKHRGYVFGSLIWEDGKHHVRSPIVVNLG 747

BLAST of Sgr022088 vs. ExPASy TrEMBL
Match: A0A6J1CQB4 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013335 PE=3 SV=1)

HSP 1 Score: 1072.8 bits (2773), Expect = 5.7e-310
Identity = 555/778 (71.34%), Postives = 638/778 (82.01%), Query Frame = 0

Query: 1   MEAFNLSSLLLLFFLFALLLTSTIAILHRLLGITFTWVGSFS-GRSPTRNRISL----QF 60
           ME FN SSLLLLFFLFA+L TST A     +     ++GS S G +P+   + L     +
Sbjct: 1   MEGFNHSSLLLLFFLFAVLQTSTFATKKSYI----FYLGSHSHGLNPSAIDLQLATESHY 60

Query: 61  TRVVARKYSNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTT 120
             + +   SN AAK+AIFYSYN++INGFAA+LD KVA++LAKHP+VVSVHEN+ R+LHTT
Sbjct: 61  NLLGSLLGSNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTT 120

Query: 121 SSWNFLGVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGS 180
            SW+FLG+E+DG  PSNSIWNLASFGESTII NLDTGVWPESKSFSD+GYGPIPSRWRGS
Sbjct: 121 GSWSFLGLENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGS 180

Query: 181 CEGGSKFHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGAS 240
           CEGGSKF CNRKLIGARYFNKGY A +GPL+ SYETARD EGHGTHTLSTAGGNF+SGAS
Sbjct: 181 CEGGSKFRCNRKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGAS 240

Query: 241 VFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGG 300
           VFGNGNGTAKGGSPRARV AY+VCWP +L +GGCF AD LA  EAAI DGVDVLS+SLGG
Sbjct: 241 VFGNGNGTAKGGSPRARVAAYRVCWPAVL-TGGCFMADILAGFEAAIHDGVDVLSVSLGG 300

Query: 301 GSKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSY 360
             ++FSDD  AIGAFHAVQHGI VVCSAGNSGP   TV NVAPW++TVGAST +RLF SY
Sbjct: 301 SPEEFSDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASY 360

Query: 361 VALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKI 420
           V LGN+KHIKGASLSDKILPAQKFYPLIS ADAKA+NVS++ AQ C EGSLDP+KV+GKI
Sbjct: 361 VGLGNRKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKI 420

Query: 421 IVCLRGDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNG-------- 480
           +VCLRGD+ RVDKGYVAAQAGAVGMILAN+++NGD L+ADAHLLPASH S          
Sbjct: 421 VVCLRGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQY 480

Query: 481 LHDSRTDRA-RNQTSTNYGFILIQRSQYNRRVNTQAQNLEIVSNFFLQPDITAPGVNIIA 540
           +  ++   A      T  G   ++ + +    +++  N   +    L+PDI APGV+IIA
Sbjct: 481 IKSTKIPMAYMTHVKTELG---VKPAPFMASFSSRGPN--TIEESILKPDIIAPGVSIIA 540

Query: 541 AYSEDAPPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTA 600
           AYSE+A PSGSS+D RR PFN  SGTSMSCPH+SGIVGLLKT YPKWSPAAIKSA+MTTA
Sbjct: 541 AYSEEASPSGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTA 600

Query: 601 ETRANNFHPILNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQ 660
            T+AN+ HPILNTTQ+KANPL+YGAGHVRPN A+NPGLVYDLT+ DYLNFLCARGYN TQ
Sbjct: 601 ATKANDLHPILNTTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQ 660

Query: 661 IKKFSNTSFICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGI 720
           IKKFS+  F CS SFKLTDFNYPSISIPNLK GPV  KRRVKNVGSPGTYVA+VK P G+
Sbjct: 661 IKKFSSAPFACSHSFKLTDFNYPSISIPNLKFGPVKAKRRVKNVGSPGTYVAQVKAPPGV 720

Query: 721 SVSIEPSTLKFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNLG 765
           +VS+EP+ LKFTGI EE++F+VV++R+ N + RGYVFG LAWSDGNH VRSPIAVNLG
Sbjct: 721 AVSVEPNMLKFTGIGEEQSFRVVVRRVENEERRGYVFGWLAWSDGNHRVRSPIAVNLG 768

BLAST of Sgr022088 vs. ExPASy TrEMBL
Match: A0A6J1EWX5 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438769 PE=3 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 5.3e-307
Identity = 554/775 (71.48%), Postives = 625/775 (80.65%), Query Frame = 0

Query: 1   MEAFNLSSLLLLFFLFALLLTSTIAILHRLLGITFTWVGSFS-GRSPT----RNRISLQF 60
           ME FNLSSL+LLFFLF L  TSTIA     +     ++GS S G +P+    R      +
Sbjct: 1   MEGFNLSSLILLFFLFPLFQTSTIATQKSYI----VYLGSHSHGSNPSSVDLRIATESHY 60

Query: 61  TRVVARKYSNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTT 120
           + + +   SNE AKEAIFYSY+RHINGFAA+LD KVA+DL +HPAVVSVHEN+ R+LHTT
Sbjct: 61  SLLGSLLGSNEIAKEAIFYSYHRHINGFAAVLDHKVAQDLTRHPAVVSVHENKMRKLHTT 120

Query: 121 SSWNFLGVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGS 180
           SSW FL +E+  G+P NSIWN A+FGESTIIANLDTGVWPESKSF+DEGYG IPSRWRGS
Sbjct: 121 SSWEFLDLENGEGTPPNSIWNGANFGESTIIANLDTGVWPESKSFNDEGYGAIPSRWRGS 180

Query: 181 CEGGSKFHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGAS 240
           CEGGS FHCNRKLIGARYFNKGY A  G LD S++TARDH+GHGTHTLSTAGGNF+SGA+
Sbjct: 181 CEGGSNFHCNRKLIGARYFNKGYTALAGSLDDSFDTARDHDGHGTHTLSTAGGNFVSGAN 240

Query: 241 VFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGG 300
           VFGNGNGTAKGGSP+A V AYKVCWP   + G C DAD LAAIEAAI+DGVDVLS+SLG 
Sbjct: 241 VFGNGNGTAKGGSPKAFVAAYKVCWP-TFHGGQCSDADILAAIEAAITDGVDVLSLSLGR 300

Query: 301 GSKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSY 360
           GS +F DDVTAIG+FHAVQ GI+VVCS GNSGP   +VENVAPW+ TV ASTI R FTSY
Sbjct: 301 GSTEFFDDVTAIGSFHAVQQGIVVVCSGGNSGPDPQSVENVAPWLFTVAASTITRQFTSY 360

Query: 361 VALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKI 420
           VALGNKKHI GAS+SDKILPAQ+FYPLI++ DAKA N+SV+ A+ CVEGSLDP KVKGKI
Sbjct: 361 VALGNKKHITGASISDKILPAQQFYPLITSVDAKAINISVETAKLCVEGSLDPRKVKGKI 420

Query: 421 IVCLR-GDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRTD 480
           IVC++ GDS RVDKGYVAAQAGAVGMILANSEE+G+ LIADAHLLP SH S  +      
Sbjct: 421 IVCVKGGDSARVDKGYVAAQAGAVGMILANSEEDGNELIADAHLLPVSHISY-IDGEIVY 480

Query: 481 RARNQTSTNYGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYS 540
              N T T   ++   R++   +      +  ++    +    L+PDITAPGVNIIAAYS
Sbjct: 481 EYINSTKTPVAYMTQVRTETGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYS 540

Query: 541 EDAPPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETR 600
           EDA PSGS +DNRRIPFNVVSGTSMSCPHISGIV LLKT+YPKWSPAAIKSAIMTTAETR
Sbjct: 541 EDASPSGSPFDNRRIPFNVVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETR 600

Query: 601 ANNFHPILNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKK 660
           AN+ HPIL++T++ ANPLAYGAGHV PN A NPGLVYDLT  DYLNFLCARGYN TQ+ K
Sbjct: 601 ANDLHPILDSTKLVANPLAYGAGHVHPNRAANPGLVYDLTTNDYLNFLCARGYNKTQLSK 660

Query: 661 FSNTSFICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVS 720
           FSNTSF+CSKSFKLTDFNYPSISIP +K  PV IKR VKNVGSP TYVARVKVP G+ VS
Sbjct: 661 FSNTSFVCSKSFKLTDFNYPSISIPKMKSEPVTIKRTVKNVGSPSTYVARVKVPPGVLVS 720

Query: 721 IEPSTLKFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNLG 765
           +EPSTLKFT  DEEKTFKVV + + N KHRGYVFGSL W DG HHVRS I VNLG
Sbjct: 721 VEPSTLKFTRTDEEKTFKVVFRSVTNTKHRGYVFGSLKWLDGKHHVRSSIVVNLG 769

BLAST of Sgr022088 vs. ExPASy TrEMBL
Match: A0A6J1KZG3 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111500243 PE=3 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.6e-300
Identity = 540/775 (69.68%), Postives = 627/775 (80.90%), Query Frame = 0

Query: 1   MEAFNLSSLLLLFFLFALLLTSTIAILHRLLGITFTWVGSFS-GRSPTRNRISL----QF 60
           ME FNLSSL+LLFF F LL TSTIA     +     ++GS S G +P+   + +     +
Sbjct: 1   MEGFNLSSLILLFFFFPLLQTSTIATQKSYI----VYLGSHSHGLNPSSADLQVATESHY 60

Query: 61  TRVVARKYSNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTT 120
           + + +   SNE AKEAIFYSYN++INGFAA+LD KV +DLA+HPAVVS+HEN+ R+LHTT
Sbjct: 61  SLLGSLLGSNEMAKEAIFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTT 120

Query: 121 SSWNFLGVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGS 180
           +SW FL +E+  G+P NSIWN ++FGESTIIANLDTGVWPESKSFSDEGY  IPSRWRGS
Sbjct: 121 NSWEFLELENGEGTPPNSIWNASNFGESTIIANLDTGVWPESKSFSDEGYEAIPSRWRGS 180

Query: 181 CEGGSKFHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGAS 240
           CEGGS FHCN+KLIGARYFNKGY A  G LD S++TARD EGHGTHTLSTAGG+F+SGA+
Sbjct: 181 CEGGSNFHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGAN 240

Query: 241 VFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGG-CFDADNLAAIEAAISDGVDVLSMSLG 300
           VFG GNGTAKGGSP+ARV AYKVCW  L + GG C DAD LAAIEAAI+DGVDVLS+SLG
Sbjct: 241 VFGYGNGTAKGGSPKARVAAYKVCW--LTFRGGQCSDADILAAIEAAITDGVDVLSLSLG 300

Query: 301 GGSKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTS 360
            GS +F DDV AIGAFHAVQ G++VVC+ GNSGP L +VENVAPW+ TV ASTINR FTS
Sbjct: 301 RGSMEFFDDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTS 360

Query: 361 YVALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGK 420
           YVALGN+K+I GASLSDKIL AQ+FYPLI++ADAKA NVSV+ A+ CVEGSLDP KVKGK
Sbjct: 361 YVALGNEKNISGASLSDKILSAQQFYPLITSADAKAINVSVEIAKLCVEGSLDPRKVKGK 420

Query: 421 IIVCLRGDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRTD 480
           IIVC+RGD  RV+KG+VAA+AGAVGMILAN+EE G+ ++ADAH+LP SH S  +      
Sbjct: 421 IIVCVRGDDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHISY-VDGETVY 480

Query: 481 RARNQTSTNYGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYS 540
              N T T   ++   R++   +      +  ++    +    L+PDITAPG+NIIAAYS
Sbjct: 481 EYINSTKTPVAYMTHVRTEMGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYS 540

Query: 541 EDAPPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETR 600
           +DA PSGS +DNRRIPFN+VSGTSMSCPHISGIV LLKT+YPKWSPAAIKSAIMTTAETR
Sbjct: 541 KDASPSGSPFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETR 600

Query: 601 ANNFHPILNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKK 660
           AN+ HPIL+ T++ ANPLAYGAGHV+PN A NPGLVYDLT  DYLNFLCARGYN  Q+ K
Sbjct: 601 ANDLHPILDFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSK 660

Query: 661 FSNTSFICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVS 720
           FSNTSF+CSKSFKLTDFNYPSISIPN+K GPV IKR VKNVGSP TYVARV+VP G+ VS
Sbjct: 661 FSNTSFVCSKSFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVS 720

Query: 721 IEPSTLKFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNLG 765
           I+PSTLKFT  DEEKTF+VV Q +ANNKHRGYVFGSL W DG HHVRS I VNLG
Sbjct: 721 IKPSTLKFTRTDEEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVVNLG 768

BLAST of Sgr022088 vs. ExPASy TrEMBL
Match: A0A5D3D7B7 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00140 PE=3 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 4.8e-300
Identity = 524/706 (74.22%), Postives = 584/706 (82.72%), Query Frame = 0

Query: 64  SNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGV 123
           SNEAAKEAIFYSYNRHINGFAA+LD KV EDLA++PAV S+HEN+ R+LHTTSSW FLGV
Sbjct: 40  SNEAAKEAIFYSYNRHINGFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGV 99

Query: 124 EHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSKFH 183
           EHD G P NSIWN ASFGESTII NLDTGVWPESKSF+DEGYGP+P+RW+GSCEGGSKFH
Sbjct: 100 EHDDGIPHNSIWNRASFGESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFH 159

Query: 184 CNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGASVFGNGNGT 243
           CNRKLIGARYFNKGYAAY G L+ SYETARD+EGHGTHTLSTAGGNFISGA+VFGNGNGT
Sbjct: 160 CNRKLIGARYFNKGYAAYAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGT 219

Query: 244 AKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGGGSKDFSDD 303
           AKGGSP+A V AYKVCWPQ+   GGCFDAD LAAIEAAISDGVD+LS+SLGGG+KDFS+D
Sbjct: 220 AKGGSPKALVAAYKVCWPQVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSED 279

Query: 304 VTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVALGNKKH 363
           VTAIGAFHAVQ GIIVVCSAGNSGP+  T+EN APW++TVGASTINR FTSYVALGNKKH
Sbjct: 280 VTAIGAFHAVQQGIIVVCSAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKH 339

Query: 364 IKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKIIVCLRGDS 423
           IK   L   IL    +    S      N  S    Q C  GSLDP+KVKGKII+CLRG++
Sbjct: 340 IKVLFLIKFILMGSSY----SIEKTLMNPCS----QLCQAGSLDPKKVKGKIILCLRGEN 399

Query: 424 GRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRTDRARNQTSTN 483
            RVDKGY AAQAGAVGMILAN+E+NGD LIADAHLLP SH S         +  N T T 
Sbjct: 400 ARVDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSY-TDGQSIYQYINFTKTP 459

Query: 484 YGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSEDAPPSGSS 543
             ++   R++   +      +  ++    +    L+PDITAPGVNI+AAYSEDA PSGS 
Sbjct: 460 MAYMTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSL 519

Query: 544 YDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPILN 603
           +DNRRIPFN+VSGTSMSCPHISGIVGLLKTLYP WSPAAIKSAIMTTAETRAN+ HPILN
Sbjct: 520 FDNRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAETRANDLHPILN 579

Query: 604 TTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKFSNTSFICS 663
           T  +KANP AYGAGHV+PN A+NPGLVYDLT  DY+NFLCA+GYN +QI KFS TSF+CS
Sbjct: 580 TVNLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCS 639

Query: 664 KSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVSIEPSTLKFT 723
           KSFKLTDFNYPSISIP++K G V I RRVKNVG P TYVARVKVP+G+SVS+EP TLKFT
Sbjct: 640 KSFKLTDFNYPSISIPDMKSGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFT 699

Query: 724 GIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNLG 765
           GIDEEK+FKVV+  +ANNKHRGYVFGSL W DG HHVRSPI VNLG
Sbjct: 700 GIDEEKSFKVVIGSVANNKHRGYVFGSLIWEDGKHHVRSPIVVNLG 736

BLAST of Sgr022088 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 786.6 bits (2030), Expect = 1.8e-227
Identity = 404/709 (56.98%), Postives = 502/709 (70.80%), Query Frame = 0

Query: 64  SNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGV 123
           S E A +AIFYSY +HINGFAA LD  +A +++KHP VVSV  N+  +LHTT SW+FLG+
Sbjct: 67  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126

Query: 124 EHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEG--GSK 183
           EH+   PS+SIW  A FGE TIIANLDTGVWPESKSF DEG GPIPSRW+G C+    + 
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 186

Query: 184 FHCNRKLIGARYFNKGYAAYVGPLDTSYETARDHEGHGTHTLSTAGGNFISGASVFGNGN 243
           FHCNRKLIGARYFNKGYAA VG L++S+++ RD +GHG+HTLSTA G+F+ G S+FG GN
Sbjct: 187 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGN 246

Query: 244 GTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGGGSKDFS 303
           GTAKGGSPRARV AYKVCWP  +    C+DAD LAA +AAI DG DV+S+SLGG    F 
Sbjct: 247 GTAKGGSPRARVAAYKVCWPP-VKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFF 306

Query: 304 DDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVALGNK 363
           +D  AIG+FHA +  I+VVCSAGNSGP+ +TV NVAPW +TVGAST++R F S + LGN 
Sbjct: 307 NDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNG 366

Query: 364 KHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKIIVCLRG 423
           KH KG SLS   LP  KFYP++++ +AKA N S  DAQ C  GSLDP K KGKI+VCLRG
Sbjct: 367 KHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRG 426

Query: 424 DSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDS-RTDRARNQT 483
            +GRV+KG   A  G +GM+L N+   G+ L+AD H+LPA+  ++   DS    R  +QT
Sbjct: 427 QNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTS--KDSFAVSRYISQT 486

Query: 484 STNYGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSEDAPPS 543
                 I   R+    +      +  ++   IV+   L+PDITAPGV++IAAY+    P+
Sbjct: 487 KKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPT 546

Query: 544 GSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHP 603
              +D RR+ FN +SGTSMSCPHISGI GLLKT YP WSPAAI+SAIMTTA    +   P
Sbjct: 547 NEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGP 606

Query: 604 ILNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKFSNTSF 663
           I N T +KA P ++GAGHV+PN AVNPGLVYDL I DYLNFLC+ GYN +QI  FS  +F
Sbjct: 607 IQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNF 666

Query: 664 ICSK-SFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVSIEPST 723
            CS     L + NYPSI++PNL    V + R VKNVG P  Y  +V  P+G+ V+++P++
Sbjct: 667 TCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTS 726

Query: 724 LKFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNL 764
           L FT + E+KTFKV+L +   N  +GYVFG L WSD  H VRSPI V L
Sbjct: 727 LNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Sgr022088 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 776.9 bits (2005), Expect = 1.5e-224
Identity = 408/708 (57.63%), Postives = 505/708 (71.33%), Query Frame = 0

Query: 64  SNEAAKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGV 123
           S+E AKEAIFYSY RHINGFAA+LD   A ++AKHP VVSV  N+ R+LHTT SWNF+ +
Sbjct: 77  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 136

Query: 124 EHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSKFH 183
             +G    +S+WN A +GE TIIANLDTGVWPESKSFSDEGYG +P+RW+G C       
Sbjct: 137 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVP 196

Query: 184 CNRKLIGARYFNKGYAAYVG-PLDTSYETARDHEGHGTHTLSTAGGNFISGASVFGNGNG 243
           CNRKLIGARYFNKGY AY G P + SYET RDH+GHG+HTLSTA GNF+ GA+VFG GNG
Sbjct: 197 CNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNG 256

Query: 244 TAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGGGSKDFSD 303
           TA GGSP+ARV AYKVCWP  +    CFDAD LAAIEAAI DGVDVLS S+GG + D+  
Sbjct: 257 TASGGSPKARVAAYKVCWPP-VDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMS 316

Query: 304 DVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVALGNKK 363
           D  AIG+FHAV++G+ VVCSAGNSGP   TV NVAPWV+TVGAS+++R F ++V L N +
Sbjct: 317 DGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQ 376

Query: 364 HIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKIIVCLRGD 423
             KG SLS K LP +K Y LISAADA   N +V DA  C +GSLDP+KVKGKI+VCLRGD
Sbjct: 377 SFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGD 436

Query: 424 SGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRT-DRARNQTS 483
           + RVDKG  AA AGA GM+L N + +G+ +I+DAH+LPAS       D  T     + T 
Sbjct: 437 NARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQID--YKDGETLFSYLSSTK 496

Query: 484 TNYGFILIQRSQYNRR-----VNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSEDAPPSG 543
              G+I    +  N +      +  ++    ++   L+PDITAPGVNIIAA++E   P+ 
Sbjct: 497 DPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTD 556

Query: 544 SSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPI 603
              DNRR PFN  SGTSMSCPHISG+VGLLKTL+P WSPAAI+SAIMTT+ TR N   P+
Sbjct: 557 LDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPM 616

Query: 604 LNTTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKFS-NTSF 663
           ++ +  KANP +YG+GHV+PN A +PGLVYDLT  DYL+FLCA GYN T ++ F+ +  +
Sbjct: 617 VDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQY 676

Query: 664 ICSKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARVKVPRGISVSIEPSTL 723
            C +   L DFNYPSI++PNL  G + + R++KNVG P TY AR + P G+ VS+EP  L
Sbjct: 677 TCRQGANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQL 736

Query: 724 KFTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVNL 764
            F    E K F++ L+ +      GYVFG L W+D +H+VRSPI V L
Sbjct: 737 TFNKTGEVKIFQMTLRPLPVTP-SGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Sgr022088 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 573.2 bits (1476), Expect = 3.2e-163
Identity = 320/706 (45.33%), Postives = 439/706 (62.18%), Query Frame = 0

Query: 72  IFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGVEHDGGSPS 131
           + Y+Y   I+GF+  L  + A+ L   P V+SV      +LHTT +  FLG++      +
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 132 NSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSKFH---CNRKL 191
             ++  A      ++  LDTGVWPESKS+SDEG+GPIPS W+G CE G+ F    CNRKL
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 192 IGARYFNKGYAAYVGPLDTSYE--TARDHEGHGTHTLSTAGGNFISGASVFGNGNGTAKG 251
           IGAR+F +GY + +GP+D S E  + RD +GHGTHT STA G+ + GAS+ G  +GTA+G
Sbjct: 185 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 252 GSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGGGSKDFSDDVTA 311
            +PRARV  YKVCW      GGCF +D LAAI+ AI+D V+VLSMSLGGG  D+  D  A
Sbjct: 245 MAPRARVAVYKVCW-----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 304

Query: 312 IGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVALGNKKHIKG 371
           IGAF A++ GI+V CSAGN+GPS +++ NVAPW+ TVGA T++R F +   LGN K+  G
Sbjct: 305 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 364

Query: 372 ASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVKGKIIVCLRGDSGRV 431
            SL        K  P I A +A     +  +   C+ G+L PEKVKGKI++C RG + RV
Sbjct: 365 VSLFKGEALPDKLLPFIYAGNAS----NATNGNLCMTGTLIPEKVKGKIVMCDRGINARV 424

Query: 432 DKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGL-----HDSRTDRARNQTS 491
            KG V   AG VGMILAN+  NG+ L+ADAHLLPA+           H   TD     + 
Sbjct: 425 QKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASI 484

Query: 492 TNYGFIL-IQRSQYNRRVNTQAQNLEIVSNFFLQPDITAPGVNIIAAYSEDAPPSGSSYD 551
           +  G ++ ++ S      +++  N  I  N  L+PD+ APGVNI+AA++  A P+G + D
Sbjct: 485 SILGTVVGVKPSPVVAAFSSRGPN-SITPN-ILKPDLIAPGVNILAAWTGAAGPTGLASD 544

Query: 552 NRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPILN-T 611
           +RR+ FN++SGTSMSCPH+SG+  LLK+++P+WSPAAI+SA+MTTA     +  P+L+  
Sbjct: 545 SRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA 604

Query: 612 TQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKFSNTSFIC-- 671
           T   + P  +GAGHV P  A NPGL+YDLT  DYL FLCA  Y   QI+  S  ++ C  
Sbjct: 605 TGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDP 664

Query: 672 SKSFKLTDFNYPSISIPNLKPGPVIIKRRVKNVGSPGTYVARV-KVPRGISVSIEPSTLK 731
           SKS+ + D NYPS ++     G     R V +VG  GTY  +V     G+ +S+EP+ L 
Sbjct: 665 SKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 724

Query: 732 FTGIDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSPIAVN 763
           F   +E+K++ V     ++       FGS+ WSDG H V SP+A++
Sbjct: 725 FKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Sgr022088 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 515.0 bits (1325), Expect = 1.0e-145
Identity = 301/725 (41.52%), Postives = 438/725 (60.41%), Query Frame = 0

Query: 63  YSNEAAKEA-IFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFL 122
           YS E A+E+ I + Y+   +GF+A++    A++L  HPAV++V E+R R+LHTT S  FL
Sbjct: 48  YSTEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFL 107

Query: 123 GVEHDGGSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSK 182
           G+++  G     +W+ + +G   II   DTG+WPE +SFSD   GPIP RWRG CE G++
Sbjct: 108 GLQNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGAR 167

Query: 183 F---HCNRKLIGARYFNKG-YAAYVGPLDTSYE--TARDHEGHGTHTLSTAGGNFISGAS 242
           F   +CNRK+IGAR+F KG  AA +G ++ + E  + RD +GHGTHT STA G     AS
Sbjct: 168 FSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKAS 227

Query: 243 VFGNGNGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLGG 302
           + G  +G AKG +P+AR+ AYKVCW       GC D+D LAA +AA+ DGVDV+S+S+GG
Sbjct: 228 MSGYASGVAKGVAPKARIAAYKVCWK----DSGCLDSDILAAFDAAVRDGVDVISISIGG 287

Query: 303 G---SKDFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLF 362
           G   +  +  D  AIG++ A   GI V  SAGN GP+  +V N+APWV TVGASTI+R F
Sbjct: 288 GDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNF 347

Query: 363 TSYVALGNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDDAQKCVEGSLDPEKVK 422
            +   LG+   ++G SL   +    + +P++    +  ++ S+     C+E +LDP++V+
Sbjct: 348 PADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSASL-----CMENTLDPKQVR 407

Query: 423 GKIIVCLRGDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPA----SHNSNGL 482
           GKI++C RG S RV KG V  +AG VGMILAN   NG+ L+ DAHL+PA    S+  + +
Sbjct: 408 GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRI 467

Query: 483 HDSRTDRARNQTSTNYGFILIQRSQYNRRVNTQAQNLEIVSNFFLQPDITAPGVNIIAAY 542
               +       S ++   ++         +   +    +S   L+PD+ APGVNI+AA+
Sbjct: 468 KAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAW 527

Query: 543 SEDAPPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAET 602
           ++   P+G   D R+  FN++SGTSM+CPH+SG   LLK+ +P WSPA I+SA+MTT   
Sbjct: 528 TDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNL 587

Query: 603 RANNFHPILN-TTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQI 662
             N+   +++ +T   A P  YG+GH+    A+NPGLVYD+T  DY+ FLC+ GY    I
Sbjct: 588 VDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTI 647

Query: 663 KKFSNTSFICSKSFKLT--DFNYPSIS--IPNLKPGPV--IIKRRVKNVG-SPGTYVARV 722
           +  + T   C  + K +  + NYPSI+   P  + G V   + R   NVG +   Y AR+
Sbjct: 648 QVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARI 707

Query: 723 KVPRGISVSIEPSTLKFTGIDEEKTFKVVLQRIANN---KHRGYVFGSLAWSDGNHH-VR 762
           + PRG++V+++P  L FT   + +++ V +     N      G VFGS+ W DG  H VR
Sbjct: 708 ESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVR 758

BLAST of Sgr022088 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 501.1 bits (1289), Expect = 1.5e-141
Identity = 314/786 (39.95%), Postives = 444/786 (56.49%), Query Frame = 0

Query: 8   SLLLLFFLFALLLTSTIAILHRLLGITFTWVGSFSGRSPTRNRISLQFTRVVARKYSNEA 67
           SL  +FF+F LLL           G+    V       P+       +   + R   +  
Sbjct: 5   SLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSP 64

Query: 68  AKEAIFYSYNRHINGFAAMLDVKVAEDLAKHPAVVSVHENRERQLHTTSSWNFLGVEHDG 127
               + YSY+R ++GF+A L       L +HP+V+SV  ++ R++HTT +  FLG   + 
Sbjct: 65  QPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNS 124

Query: 128 GSPSNSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPIPSRWRGSCEGGSKF---HC 187
           G     +W+ +++GE  I+  LDTG+WPE  SFSD G GPIPS W+G CE G  F    C
Sbjct: 125 G-----LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSC 184

Query: 188 NRKLIGARYFNKGYAAYVGPLD----TSYETARDHEGHGTHTLSTAGGNFISGASVFGNG 247
           NRKLIGAR F +GY                + RD EGHGTHT STA G+ ++ AS++   
Sbjct: 185 NRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYA 244

Query: 248 NGTAKGGSPRARVVAYKVCWPQLLYSGGCFDADNLAAIEAAISDGVDVLSMSLG--GGSK 307
            GTA G + +AR+ AYK+CW     +GGC+D+D LAA++ A++DGV V+S+S+G  G + 
Sbjct: 245 RGTATGMASKARIAAYKICW-----TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 304

Query: 308 DFSDDVTAIGAFHAVQHGIIVVCSAGNSGPSLATVENVAPWVVTVGASTINRLFTSYVAL 367
           ++  D  AIGAF A +HGI+V CSAGNSGP+  T  N+APW++TVGAST++R F +    
Sbjct: 305 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 364

Query: 368 GNKKHIKGASLSDKILPAQKFYPLISAADAKANNVSVDD--AQKCVEGSLDPEKVKGKII 427
           G+ K   G SL          Y   S  D++ + V   D  ++ C  G L+   V+GKI+
Sbjct: 365 GDGKVFTGTSL----------YAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIV 424

Query: 428 VCLRGDSGRVDKGYVAAQAGAVGMILANSEENGDSLIADAHLLPASHNSNGLHDSRTD-- 487
           +C RG + RV+KG     AG  GMILAN+ E+G+ L AD+HL+PA+       D   D  
Sbjct: 425 LCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYI 484

Query: 488 RARNQTSTNYGFI--LIQRSQYNRRVNT-QAQNLEIVSNFFLQPDITAPGVNIIAAYSED 547
           +  +  +    F+  LI  S  + RV    ++    ++   L+PD+ APGVNI+A ++  
Sbjct: 485 KTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGM 544

Query: 548 APPSGSSYDNRRIPFNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRAN 607
             P+    D RR+ FN++SGTSMSCPH+SG+  LL+  +P WSPAAIKSA++TTA    N
Sbjct: 545 VGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVEN 604

Query: 608 NFHPILN-TTQVKANPLAYGAGHVRPNGAVNPGLVYDLTITDYLNFLCARGYNGTQIKKF 667
           +  PI +  T   +N   +GAGHV PN A+NPGLVYD+ + +Y+ FLCA GY    I  F
Sbjct: 605 SGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVF 664

Query: 668 --SNTSFICSKSFKLT---DFNYPSISIPNLKPGPVI-IKRRVKNVGS--PGTYVARVKV 727
               T +   ++ KL    D NYPS S+     G V+  KR VKNVGS     Y   VK 
Sbjct: 665 LQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKS 724

Query: 728 PRGISVSIEPSTLKFTG----IDEEKTFKVVLQRIANNKHRGYVFGSLAWSDGNHHVRSP 765
           P  + + + PS L F+     ++ E TFK V+         G+ FGS+ W+DG H V+SP
Sbjct: 725 PANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSP 770