Sgr022077 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr022077 vs. NCBI nr
Match: XP_022143472.1 (chloride channel protein CLC-f isoform X2 [Momordica charantia])

HSP 1 Score: 930.6 bits (2404), Expect = 6.2e-267
Identity = 473/498 (94.98%), Postives = 487/498 (97.79%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAEGEFSDQNRLLRS EDH+GEDHDLESQDG+GVH HRNNSGKRGGFLDLFQHLNRG+SF
Sbjct: 1   MAEGEFSDQNRLLRSMEDHSGEDHDLESQDGVGVHFHRNNSGKRGGFLDLFQHLNRGNSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSRAASTSSDRHNNFHSLPHHAPTATDGETED 158
           SGRRLSYKR++MDNH+V+Y PSSVNIIGRSRAASTSSDRHNNF+S PHHAPTA DGE +D
Sbjct: 61  SGRRLSYKRIEMDNHNVNYNPSSVNIIGRSRAASTSSDRHNNFNSHPHHAPTAIDGEIDD 120

Query: 159 NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD 218
           NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD
Sbjct: 121 NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD 180

Query: 219 TWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAITLG 278
           TWHRILLIPVTGGVIVGMMHGLLEIL QIKQSS+ Q QGFDLL+GVFPTVKAIQAAITLG
Sbjct: 181 TWHRILLIPVTGGVIVGMMHGLLEILCQIKQSSSPQSQGFDLLSGVFPTVKAIQAAITLG 240

Query: 279 TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFA 338
           TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKI+IAFVAAGAAAGIASGFNAAVAGSFFA
Sbjct: 241 TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIRIAFVAAGAAAGIASGFNAAVAGSFFA 300

Query: 339 IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYLI 398
           IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVP YDLKSAAELPLYLI
Sbjct: 301 IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPAYDLKSAAELPLYLI 360

Query: 399 LGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWGF 458
           LGMLCGVVSVAVTRLVAWFGK FEF+KERFGLPPVVCPALGGLGAGIIALKYPGILYWGF
Sbjct: 361 LGMLCGVVSVAVTRLVAWFGKSFEFVKERFGLPPVVCPALGGLGAGIIALKYPGILYWGF 420

Query: 459 TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFG 518
           TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA+FG
Sbjct: 421 TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAIFG 480

Query: 519 GSAVEIINIAIPGNAAVA 537
           GSAVEIINIAIPGNAAVA
Sbjct: 481 GSAVEIINIAIPGNAAVA 498

BLAST of Sgr022077 vs. NCBI nr
Match: XP_022143471.1 (chloride channel protein CLC-f isoform X1 [Momordica charantia])

HSP 1 Score: 930.6 bits (2404), Expect = 6.2e-267
Identity = 473/498 (94.98%), Postives = 487/498 (97.79%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAEGEFSDQNRLLRS EDH+GEDHDLESQDG+GVH HRNNSGKRGGFLDLFQHLNRG+SF
Sbjct: 1   MAEGEFSDQNRLLRSMEDHSGEDHDLESQDGVGVHFHRNNSGKRGGFLDLFQHLNRGNSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSRAASTSSDRHNNFHSLPHHAPTATDGETED 158
           SGRRLSYKR++MDNH+V+Y PSSVNIIGRSRAASTSSDRHNNF+S PHHAPTA DGE +D
Sbjct: 61  SGRRLSYKRIEMDNHNVNYNPSSVNIIGRSRAASTSSDRHNNFNSHPHHAPTAIDGEIDD 120

Query: 159 NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD 218
           NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD
Sbjct: 121 NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD 180

Query: 219 TWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAITLG 278
           TWHRILLIPVTGGVIVGMMHGLLEIL QIKQSS+ Q QGFDLL+GVFPTVKAIQAAITLG
Sbjct: 181 TWHRILLIPVTGGVIVGMMHGLLEILCQIKQSSSPQSQGFDLLSGVFPTVKAIQAAITLG 240

Query: 279 TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFA 338
           TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKI+IAFVAAGAAAGIASGFNAAVAGSFFA
Sbjct: 241 TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIRIAFVAAGAAAGIASGFNAAVAGSFFA 300

Query: 339 IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYLI 398
           IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVP YDLKSAAELPLYLI
Sbjct: 301 IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPAYDLKSAAELPLYLI 360

Query: 399 LGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWGF 458
           LGMLCGVVSVAVTRLVAWFGK FEF+KERFGLPPVVCPALGGLGAGIIALKYPGILYWGF
Sbjct: 361 LGMLCGVVSVAVTRLVAWFGKSFEFVKERFGLPPVVCPALGGLGAGIIALKYPGILYWGF 420

Query: 459 TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFG 518
           TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA+FG
Sbjct: 421 TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAIFG 480

Query: 519 GSAVEIINIAIPGNAAVA 537
           GSAVEIINIAIPGNAAVA
Sbjct: 481 GSAVEIINIAIPGNAAVA 498

BLAST of Sgr022077 vs. NCBI nr
Match: XP_031739481.1 (chloride channel protein CLC-f [Cucumis sativus] >KGN55984.1 hypothetical protein Csa_010834 [Cucumis sativus])

HSP 1 Score: 900.6 bits (2326), Expect = 6.8e-258
Identity = 465/499 (93.19%), Postives = 476/499 (95.39%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAE EFSDQNRLLRS EDHN EDHDLESQD  GVHP RNNSGKRGGFLDL  HLNRG SF
Sbjct: 1   MAEEEFSDQNRLLRSMEDHNEEDHDLESQDANGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSR-AASTSSDRHNNFHSLPHHAPTATDGETE 158
           SGRRLSYKRLDMDNH+V++ PSSVNI+GRSR A+S+SSDRHNNF+S   H+PTA DGE +
Sbjct: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTASSSSSDRHNNFNSYSLHSPTAIDGEID 120

Query: 159 DNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMA 218
           DN DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMA
Sbjct: 121 DNGDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMA 180

Query: 219 DTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAITL 278
           DTWHRILLIPV GGVIVGMMHGLLEIL+QIKQSS SQ QGFDLL+GVFPTVKAIQAAITL
Sbjct: 181 DTWHRILLIPVCGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAITL 240

Query: 279 GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF 338
           GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF
Sbjct: 241 GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF 300

Query: 339 AIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYL 398
           AIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYL
Sbjct: 301 AIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYL 360

Query: 399 ILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 458
           ILGMLCG VSVAVTRLVAWFG  FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG
Sbjct: 361 ILGMLCGAVSVAVTRLVAWFGNSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 420

Query: 459 FTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVF 518
           FTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVF
Sbjct: 421 FTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVF 480

Query: 519 GGSAVEIINIAIPGNAAVA 537
           GGSAVEIINIAIPGNAAVA
Sbjct: 481 GGSAVEIINIAIPGNAAVA 499

BLAST of Sgr022077 vs. NCBI nr
Match: XP_008448989.1 (PREDICTED: chloride channel protein CLC-f [Cucumis melo])

HSP 1 Score: 900.2 bits (2325), Expect = 8.9e-258
Identity = 465/500 (93.00%), Postives = 477/500 (95.40%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAE EFSDQNRLLRS EDHN EDHDLESQD  GVHP RNNSGKRGGFLDL  HLNRG SF
Sbjct: 1   MAEEEFSDQNRLLRSMEDHNEEDHDLESQDVNGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSRAA--STSSDRHNNFHSLPHHAPTATDGET 158
           SGRRLSYKR+DMDNH+V+++PSSVNI+GRSR A  S+SSDRHNNF+S   H+PTA DGE 
Sbjct: 61  SGRRLSYKRIDMDNHNVNFSPSSVNIVGRSRTASSSSSSDRHNNFNSYSLHSPTAIDGEI 120

Query: 159 EDNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 218
           +DN DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM
Sbjct: 121 DDNGDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180

Query: 219 ADTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAIT 278
           ADTWHRILLIPV GGVIVGMMHGLLEIL+QIKQSS SQ QGFDLL+GVFPTVKAIQAAIT
Sbjct: 181 ADTWHRILLIPVCGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240

Query: 279 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 338
           LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF
Sbjct: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300

Query: 339 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY 398
           FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY
Sbjct: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY 360

Query: 399 LILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 458
           LILGMLCG VSVAVTRLVAWFGK FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW
Sbjct: 361 LILGMLCGAVSVAVTRLVAWFGKSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420

Query: 459 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 518
           GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV
Sbjct: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480

Query: 519 FGGSAVEIINIAIPGNAAVA 537
           FGGSAVEIINIAIPGNAAVA
Sbjct: 481 FGGSAVEIINIAIPGNAAVA 500

BLAST of Sgr022077 vs. NCBI nr
Match: XP_038904981.1 (chloride channel protein CLC-f [Benincasa hispida])

HSP 1 Score: 899.8 bits (2324), Expect = 1.2e-257
Identity = 464/499 (92.99%), Postives = 478/499 (95.79%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAEGEFSDQNRLLRS EDHN ED DLESQDG GVHP RNNSGKRGGFLDL  HLNRG SF
Sbjct: 1   MAEGEFSDQNRLLRSMEDHNEEDLDLESQDGNGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSR-AASTSSDRHNNFHSLPHHAPTATDGETE 158
           SGRRLSYKRLD+DNH+V++ PSSVNI+GRSR A+S+SSDRHNNF+S   HAPTA DGE +
Sbjct: 61  SGRRLSYKRLDIDNHNVNFNPSSVNIVGRSRTASSSSSDRHNNFNSHSLHAPTAIDGEID 120

Query: 159 DNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMA 218
           DN+DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQR+A
Sbjct: 121 DNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRIA 180

Query: 219 DTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAITL 278
           DTWHRILLIPVTGGVIVGMMHGLLEIL+QIKQSS SQ QGFDLL+GVFPTVKAIQAAITL
Sbjct: 181 DTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAITL 240

Query: 279 GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF 338
           GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF
Sbjct: 241 GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF 300

Query: 339 AIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYL 398
           AIETVLRPLRAENSPPFTTAMIILASVISSTVS VLLGTQSAFTVP YDLKSAAELPLYL
Sbjct: 301 AIETVLRPLRAENSPPFTTAMIILASVISSTVSTVLLGTQSAFTVPAYDLKSAAELPLYL 360

Query: 399 ILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 458
           ILGMLCG VSVAVTRLVAWFGK FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG
Sbjct: 361 ILGMLCGAVSVAVTRLVAWFGKSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 420

Query: 459 FTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVF 518
           FTNVEEILHTGNRPSAPGIWLLTQIAAAKV+ATALCKGSGLVGGLYAPSLMIGAAVGAVF
Sbjct: 421 FTNVEEILHTGNRPSAPGIWLLTQIAAAKVLATALCKGSGLVGGLYAPSLMIGAAVGAVF 480

Query: 519 GGSAVEIINIAIPGNAAVA 537
           GGSAVEIINIAIPGNAAVA
Sbjct: 481 GGSAVEIINIAIPGNAAVA 499

BLAST of Sgr022077 vs. ExPASy Swiss-Prot
Match: Q8RXR2 (Chloride channel protein CLC-f OS=Arabidopsis thaliana OX=3702 GN=CLC-F PE=2 SV=2)

HSP 1 Score: 674.9 bits (1740), Expect = 8.0e-193
Identity = 371/504 (73.61%), Postives = 409/504 (81.15%), Query Frame = 0

Query: 42  GEFSDQNRLLRSTEDH-----NGE-DHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRG 101
           GE+++   LLRST+        GE D D+ESQ           SG  GG  DLF+H++R 
Sbjct: 7   GEYNEDRHLLRSTDGDEVGIGGGEGDLDVESQS------PAIRSG-AGGVRDLFKHIDRR 66

Query: 102 HSFSGRRLSYKRLDMDNHSVS---YTPSSVNIIGRSRAASTSSDRHNNFHSLPHHAPTAT 161
            S SGRRLS+KR  M+N  V    + PSS +    S A        +N HS+        
Sbjct: 67  FSLSGRRLSFKR--MENIRVDRERHNPSSSSAF--SAAGEEDGGGISNLHSVDDR--NDE 126

Query: 162 DGETEDNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLR 221
            G  E+ L D+APPEWALLLI CL+G+A G+CVA FN GVHVIHEWAWAGTPNEGAAWLR
Sbjct: 127 YGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAWLR 186

Query: 222 LQRMADTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQ 281
           LQR+ADTWHRILLIPVTGGVIVGMMHGLLEIL+QI+QS++SQRQG D LAG++P +KAIQ
Sbjct: 187 LQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGLDFLAGIYPVIKAIQ 246

Query: 282 AAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAV 341
           AA+TLGTGCSLGPEGPSVDIGKSCANGF LMMENN E+ +IA  AAGAA+GIASGFNAAV
Sbjct: 247 AAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRER-RIALTAAGAASGIASGFNAAV 306

Query: 342 AGSFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAE 401
           AG FFAIETVLRPLRAENSPPFTTAMIILASVISSTVSN LLGTQSAFTVP+YDLKSAAE
Sbjct: 307 AGCFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNALLGTQSAFTVPSYDLKSAAE 366

Query: 402 LPLYLILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPG 461
           LPLYLILGMLCG VSV  +RLV WF K F+FIK++FGLP +VCPALGGLGAGIIALKYPG
Sbjct: 367 LPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALKYPG 426

Query: 462 ILYWGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAA 521
           ILYWGFTNVEEILHTG   SAPGIWLL Q+AAAKVVATALCKGSGLVGGLYAPSLMIGAA
Sbjct: 427 ILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMIGAA 486

Query: 522 VGAVFGGSAVEIINIAIPGNAAVA 537
           VGAVFGGSA EIIN AIPGNAAVA
Sbjct: 487 VGAVFGGSAAEIINRAIPGNAAVA 496

BLAST of Sgr022077 vs. ExPASy Swiss-Prot
Match: Q8GX93 (Chloride channel protein CLC-e OS=Arabidopsis thaliana OX=3702 GN=CLC-E PE=2 SV=2)

HSP 1 Score: 297.4 bits (760), Expect = 3.5e-79
Identity = 171/391 (43.73%), Postives = 242/391 (61.89%), Query Frame = 0

Query: 149 PTATDGETEDNLD-DTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEG 208
           P A     +D +  D  P +   +  ACL+G+ TG+ V  FN  VH++ +++W G P+ G
Sbjct: 54  PAAKQETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRG 113

Query: 209 AAWLRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVF-P 268
           A+WLR   +   W R++L+P  GG++V +++ L E      +S+       D +  V  P
Sbjct: 114 ASWLREAPIGSNWLRVILVPTIGGLVVSILNQLRE---SAGKSTGDSHSSLDRVKAVLRP 173

Query: 269 TVKAIQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIAS 328
            +K + A +TLGTG SLGPEGPSV+IG S A G   +  N S +   + +AAG+AAGI+S
Sbjct: 174 FLKTVAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLF-NKSPQTGFSLLAAGSAAGISS 233

Query: 329 GFNAAVAGSFFAIETVLRPLRAENSP---PFTTAMIILASVISSTVSNVLLGTQSAFTVP 388
           GFNAAVAG FFA+E+VL P  + +S    P TT+M+IL++V +S VS + LG++ AF VP
Sbjct: 234 GFNAAVAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVP 293

Query: 389 TYDLKSAAELPLYLILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGA 448
            YD +S  ELPLYL+LG LCG+VS+A++R  +      + + +  G+P  V P +GGL  
Sbjct: 294 DYDFRSPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSV 353

Query: 449 GIIALKYPGILYWGFTNVEEILHTGNRPSAPGIW--LLTQIAAAKVVATALCKGSGLVGG 508
           GIIAL YP +LYWGF NV+ +L    RP   G+   LL Q+ A K+ ATA C+ SGLVGG
Sbjct: 354 GIIALVYPEVLYWGFQNVDILLE--KRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGG 413

Query: 509 LYAPSLMIGAAVGAVFGGSAVEIINIAIPGN 533
            YAPSL IG A G  +G    + I +A+  N
Sbjct: 414 YYAPSLFIGGAAGMAYG----KFIGLALAQN 434

BLAST of Sgr022077 vs. ExPASy Swiss-Prot
Match: Q8XTT4 (Putative chloride channel protein ClcB-like OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=RSp0020 PE=3 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 2.4e-24
Identity = 109/374 (29.14%), Postives = 177/374 (47.33%), Query Frame = 0

Query: 170 LLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMADTWHRILLIPVT 229
           +L IA L+G A  L   AF   +  + +W  AG      A  R    A  W   LL+P  
Sbjct: 1   MLAIAGLIGCAGALATIAFRECLRQL-QWWLAGADQGLVATAR----ALPWWARLLVPTA 60

Query: 230 GGVIVG--MMHGLLEILNQ-IKQSSTSQRQGFDLLAGVFPTVKAIQAAITLGTGCSLGPE 289
           GG++ G  + +GL  I  +  +    +   G  +L+     V++  +  ++ +G S+G E
Sbjct: 61  GGLLAGLTLQYGLKWIPRKGAEDYMEAIAVGDGVLSARQSLVRSASSLCSVASGASIGRE 120

Query: 290 GPSVDIGKSCAN--GFYL--MMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIETV 349
           GP V +   C +  G  L   M  + E +++  VA GAAAGI S +NA +AG+ F  E V
Sbjct: 121 GPMVQLAAMCGSLLGRVLRHAMPVSVEHMRL-LVACGAAAGITSAYNAPIAGAVFVCEIV 180

Query: 350 LRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYLILGML 409
              +      P     +++++V +  +     G  + + +P +D  S  E+  YL LG+ 
Sbjct: 181 FGAITTATLGP-----LLVSAVTADIIVRQFFGYGAVYAMPHFDFVSGWEVLTYLGLGLA 240

Query: 410 CGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWGFTNVE 469
            G+    +  L+      F     R  LP  +  ALGGL  G ++++ P +   G++ V 
Sbjct: 241 AGMAGPLLLGLIDRARGAF----ARTRLPQALRLALGGLIVGALSIRVPEVWGNGYSVVN 300

Query: 470 EILHTGNRPSAPGIW-LLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSA 529
             LH      AP +W  +  +   KV ATA   GSG VGG++ P+L  GAA+G ++G   
Sbjct: 301 GFLH------APWLWQTVALVLVCKVGATAASAGSGAVGGVFTPTLFCGAALGLLYGTG- 349

Query: 530 VEIINIAIPGNAAV 536
              ++  +PG A V
Sbjct: 361 ---MHALLPGAAPV 349

BLAST of Sgr022077 vs. ExPASy Swiss-Prot
Match: A7N6K9 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio campbellii (strain ATCC BAA-1116 / BB120) OX=338187 GN=clcA PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.1e-19
Identity = 105/398 (26.38%), Postives = 184/398 (46.23%), Query Frame = 0

Query: 163 TAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLR--LQRMADTW 222
           + P  +++L +A ++G   GL    F I VH + E     T  E   WL+  +  +   W
Sbjct: 28  STPTSFSVLFMAAIVGTLAGLVGTYFEIAVHFVSE-----TRTE---WLKSEIGSVLPLW 87

Query: 223 HRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQ-------- 282
              +LI    G +  + + L+      + +  +   G   + G    +++++        
Sbjct: 88  LAAILI---SGALAFIGYYLVN-----RFAPEASGSGIPEIEGAMDNIRSVRWWRVIPVK 147

Query: 283 ---AAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFN 342
                  LG+G  LG EGP+V +G +       +     +  + + +A+GAA G+A+ FN
Sbjct: 148 FFGGMGALGSGMVLGREGPTVQMGGAVGRMVTDIFRVKDDDTRHSLLASGAAGGLAAAFN 207

Query: 343 AAVAGSFFAIETVLRPLRAENSPPFTTAMI-ILASVISSTVSNVLL----GTQSAFTVPT 402
           A +A   F +E        E  P F  ++I I A +IS+ ++N++     G ++  T+P 
Sbjct: 208 APLAAIMFVVE--------EMRPQFRYSLISIRAVIISAIMANIVFRAINGQEAVITMPQ 267

Query: 403 YDLKSAAELPLYLILGMLCGVVSVAVTRLVA----WFGKLFEFIKERFGLPPVVCPALGG 462
           Y       L L+L+LG L GV  V   +L+      F  L +  ++R+ +   +   LGG
Sbjct: 268 YQSPELQSLWLFLLLGSLFGVFGVVFNKLITIAQDSFVALHKNDRKRYLITGTI---LGG 327

Query: 463 LGAGIIALKYPGILYWGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVG 522
           +  G++ L  P +   G   + +I + GN      I +L  +   +V+ T LC GSG  G
Sbjct: 328 V-FGLLLLYVPQLTGGGIGLIPDITN-GNY----SISILVMLFVGRVITTLLCFGSGAPG 387

Query: 523 GLYAPSLMIGAAVGAVFGGSAVEII-NIAI-PGNAAVA 537
           G++AP L +G   G  FG SA  ++ ++ I PG  A+A
Sbjct: 388 GIFAPMLALGTLFGYAFGASADMLLPSLTIEPGVFAIA 392

BLAST of Sgr022077 vs. ExPASy Swiss-Prot
Match: A5F0D5 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=clcA PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 9.0e-19
Identity = 93/361 (25.76%), Postives = 156/361 (43.21%), Query Frame = 0

Query: 168 WALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMADTWHRIL--- 227
           +++L ++ L+G+  GL    F   VH++ E       +E  ++L L   A      L   
Sbjct: 31  FSVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFLPLWLAAFLISAFLAFI 90

Query: 228 -------LIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAITL 287
                    P   G  +  + G ++ +  ++               V P VK       L
Sbjct: 91  GYFLVHRFAPEAAGSGIPEIEGAMDGMRPVRWWR------------VLP-VKFFGGMGAL 150

Query: 288 GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF 347
           G+G  LG EGP+V +G +       +    +E  + + +AAGAA G+A+ FNA +AG  F
Sbjct: 151 GSGMVLGREGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAAGGLAAAFNAPLAGIMF 210

Query: 348 AIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYL 407
            IE +    R       +   +I+++V ++ V  V+ G  +  T+P YD    + L L+L
Sbjct: 211 VIEEMRPQFR---YTLISVRAVIISAVAANIVFRVINGQDAVITMPQYDAPELSTLGLFL 270

Query: 408 ILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 467
           +LG L GV  V    L+     LF           ++  ++ G   G++ L  P +   G
Sbjct: 271 LLGALFGVFGVLFNYLITLAQDLFVKFHRNDRKRYLLTGSMIGGCFGLLLLYVPELTGGG 330

Query: 468 FTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVF 519
            + +  I + G      G  +L  +   ++  T LC GSG  GG++AP L +G   G  F
Sbjct: 331 ISLIPTITNGGY-----GAGILLLLFVGRIFTTLLCFGSGAPGGIFAPMLALGTLFGYAF 370

BLAST of Sgr022077 vs. ExPASy TrEMBL
Match: A0A6J1CQC2 (Chloride channel protein OS=Momordica charantia OX=3673 GN=LOC111013347 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 3.0e-267
Identity = 473/498 (94.98%), Postives = 487/498 (97.79%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAEGEFSDQNRLLRS EDH+GEDHDLESQDG+GVH HRNNSGKRGGFLDLFQHLNRG+SF
Sbjct: 1   MAEGEFSDQNRLLRSMEDHSGEDHDLESQDGVGVHFHRNNSGKRGGFLDLFQHLNRGNSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSRAASTSSDRHNNFHSLPHHAPTATDGETED 158
           SGRRLSYKR++MDNH+V+Y PSSVNIIGRSRAASTSSDRHNNF+S PHHAPTA DGE +D
Sbjct: 61  SGRRLSYKRIEMDNHNVNYNPSSVNIIGRSRAASTSSDRHNNFNSHPHHAPTAIDGEIDD 120

Query: 159 NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD 218
           NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD
Sbjct: 121 NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD 180

Query: 219 TWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAITLG 278
           TWHRILLIPVTGGVIVGMMHGLLEIL QIKQSS+ Q QGFDLL+GVFPTVKAIQAAITLG
Sbjct: 181 TWHRILLIPVTGGVIVGMMHGLLEILCQIKQSSSPQSQGFDLLSGVFPTVKAIQAAITLG 240

Query: 279 TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFA 338
           TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKI+IAFVAAGAAAGIASGFNAAVAGSFFA
Sbjct: 241 TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIRIAFVAAGAAAGIASGFNAAVAGSFFA 300

Query: 339 IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYLI 398
           IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVP YDLKSAAELPLYLI
Sbjct: 301 IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPAYDLKSAAELPLYLI 360

Query: 399 LGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWGF 458
           LGMLCGVVSVAVTRLVAWFGK FEF+KERFGLPPVVCPALGGLGAGIIALKYPGILYWGF
Sbjct: 361 LGMLCGVVSVAVTRLVAWFGKSFEFVKERFGLPPVVCPALGGLGAGIIALKYPGILYWGF 420

Query: 459 TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFG 518
           TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA+FG
Sbjct: 421 TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAIFG 480

Query: 519 GSAVEIINIAIPGNAAVA 537
           GSAVEIINIAIPGNAAVA
Sbjct: 481 GSAVEIINIAIPGNAAVA 498

BLAST of Sgr022077 vs. ExPASy TrEMBL
Match: A0A6J1CQX5 (Chloride channel protein OS=Momordica charantia OX=3673 GN=LOC111013347 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 3.0e-267
Identity = 473/498 (94.98%), Postives = 487/498 (97.79%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAEGEFSDQNRLLRS EDH+GEDHDLESQDG+GVH HRNNSGKRGGFLDLFQHLNRG+SF
Sbjct: 1   MAEGEFSDQNRLLRSMEDHSGEDHDLESQDGVGVHFHRNNSGKRGGFLDLFQHLNRGNSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSRAASTSSDRHNNFHSLPHHAPTATDGETED 158
           SGRRLSYKR++MDNH+V+Y PSSVNIIGRSRAASTSSDRHNNF+S PHHAPTA DGE +D
Sbjct: 61  SGRRLSYKRIEMDNHNVNYNPSSVNIIGRSRAASTSSDRHNNFNSHPHHAPTAIDGEIDD 120

Query: 159 NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD 218
           NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD
Sbjct: 121 NLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMAD 180

Query: 219 TWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAITLG 278
           TWHRILLIPVTGGVIVGMMHGLLEIL QIKQSS+ Q QGFDLL+GVFPTVKAIQAAITLG
Sbjct: 181 TWHRILLIPVTGGVIVGMMHGLLEILCQIKQSSSPQSQGFDLLSGVFPTVKAIQAAITLG 240

Query: 279 TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFA 338
           TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKI+IAFVAAGAAAGIASGFNAAVAGSFFA
Sbjct: 241 TGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIRIAFVAAGAAAGIASGFNAAVAGSFFA 300

Query: 339 IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYLI 398
           IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVP YDLKSAAELPLYLI
Sbjct: 301 IETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPAYDLKSAAELPLYLI 360

Query: 399 LGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWGF 458
           LGMLCGVVSVAVTRLVAWFGK FEF+KERFGLPPVVCPALGGLGAGIIALKYPGILYWGF
Sbjct: 361 LGMLCGVVSVAVTRLVAWFGKSFEFVKERFGLPPVVCPALGGLGAGIIALKYPGILYWGF 420

Query: 459 TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFG 518
           TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA+FG
Sbjct: 421 TNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAIFG 480

Query: 519 GSAVEIINIAIPGNAAVA 537
           GSAVEIINIAIPGNAAVA
Sbjct: 481 GSAVEIINIAIPGNAAVA 498

BLAST of Sgr022077 vs. ExPASy TrEMBL
Match: A0A0A0L4N0 (Chloride channel protein OS=Cucumis sativus OX=3659 GN=Csa_3G044490 PE=3 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.3e-258
Identity = 465/499 (93.19%), Postives = 476/499 (95.39%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAE EFSDQNRLLRS EDHN EDHDLESQD  GVHP RNNSGKRGGFLDL  HLNRG SF
Sbjct: 1   MAEEEFSDQNRLLRSMEDHNEEDHDLESQDANGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSR-AASTSSDRHNNFHSLPHHAPTATDGETE 158
           SGRRLSYKRLDMDNH+V++ PSSVNI+GRSR A+S+SSDRHNNF+S   H+PTA DGE +
Sbjct: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTASSSSSDRHNNFNSYSLHSPTAIDGEID 120

Query: 159 DNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMA 218
           DN DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMA
Sbjct: 121 DNGDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMA 180

Query: 219 DTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAITL 278
           DTWHRILLIPV GGVIVGMMHGLLEIL+QIKQSS SQ QGFDLL+GVFPTVKAIQAAITL
Sbjct: 181 DTWHRILLIPVCGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAITL 240

Query: 279 GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF 338
           GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF
Sbjct: 241 GTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFF 300

Query: 339 AIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYL 398
           AIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYL
Sbjct: 301 AIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYL 360

Query: 399 ILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 458
           ILGMLCG VSVAVTRLVAWFG  FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG
Sbjct: 361 ILGMLCGAVSVAVTRLVAWFGNSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 420

Query: 459 FTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVF 518
           FTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVF
Sbjct: 421 FTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVF 480

Query: 519 GGSAVEIINIAIPGNAAVA 537
           GGSAVEIINIAIPGNAAVA
Sbjct: 481 GGSAVEIINIAIPGNAAVA 499

BLAST of Sgr022077 vs. ExPASy TrEMBL
Match: A0A1S3BLN3 (Chloride channel protein OS=Cucumis melo OX=3656 GN=LOC103490996 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.3e-258
Identity = 465/500 (93.00%), Postives = 477/500 (95.40%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAE EFSDQNRLLRS EDHN EDHDLESQD  GVHP RNNSGKRGGFLDL  HLNRG SF
Sbjct: 1   MAEEEFSDQNRLLRSMEDHNEEDHDLESQDVNGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSRAA--STSSDRHNNFHSLPHHAPTATDGET 158
           SGRRLSYKR+DMDNH+V+++PSSVNI+GRSR A  S+SSDRHNNF+S   H+PTA DGE 
Sbjct: 61  SGRRLSYKRIDMDNHNVNFSPSSVNIVGRSRTASSSSSSDRHNNFNSYSLHSPTAIDGEI 120

Query: 159 EDNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 218
           +DN DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM
Sbjct: 121 DDNGDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180

Query: 219 ADTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAIT 278
           ADTWHRILLIPV GGVIVGMMHGLLEIL+QIKQSS SQ QGFDLL+GVFPTVKAIQAAIT
Sbjct: 181 ADTWHRILLIPVCGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240

Query: 279 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 338
           LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF
Sbjct: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300

Query: 339 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY 398
           FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY
Sbjct: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY 360

Query: 399 LILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 458
           LILGMLCG VSVAVTRLVAWFGK FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW
Sbjct: 361 LILGMLCGAVSVAVTRLVAWFGKSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420

Query: 459 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 518
           GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV
Sbjct: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480

Query: 519 FGGSAVEIINIAIPGNAAVA 537
           FGGSAVEIINIAIPGNAAVA
Sbjct: 481 FGGSAVEIINIAIPGNAAVA 500

BLAST of Sgr022077 vs. ExPASy TrEMBL
Match: A0A6J1KX22 (Chloride channel protein OS=Cucurbita maxima OX=3661 GN=LOC111498341 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.4e-253
Identity = 453/501 (90.42%), Postives = 474/501 (94.61%), Query Frame = 0

Query: 39  MAEGEFSDQNRLLRSTEDHNGEDHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRGHSF 98
           MAE EFSDQNRLLRS EDH GEDHDLESQDG G+ P RNNSGKRGG LD+F HLNRG SF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGGILDMFHHLNRGGSF 60

Query: 99  SGRRLSYKRLDMDNHSVSYTPSSVNIIGRSRAA---STSSDRHNNFHSLPHHAPTATDGE 158
           SGRRLS KR DMDNH+ +++P+S+NI+GR+R A   S+SSDRHNNF+S   HAPT +D E
Sbjct: 61  SGRRLSSKRHDMDNHAATFSPTSLNIVGRTRTASSSSSSSDRHNNFNSYSLHAPTVSDTE 120

Query: 159 TEDNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQR 218
            +DN+DDTA PEWALLLIACLLGLATGLCVAAFN+GVHVIHEWAWAGTPNEGAAWLRLQR
Sbjct: 121 IDDNVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQR 180

Query: 219 MADTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAI 278
           MADTWHRILLIPVTGGVIVGMMHGLLEIL+QIKQSSTSQRQGFDLL+GVFPTVKAIQAAI
Sbjct: 181 MADTWHRILLIPVTGGVIVGMMHGLLEILDQIKQSSTSQRQGFDLLSGVFPTVKAIQAAI 240

Query: 279 TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS 338
           TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS
Sbjct: 241 TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS 300

Query: 339 FFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPL 398
           FFAIETVLRPLRAENSPPFTTAMIIL+SVISSTVS VLLGTQSAFTVP YDLKSAAELPL
Sbjct: 301 FFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELPL 360

Query: 399 YLILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY 458
           YLILGMLCGVVSVAVTRLVAWF KLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY
Sbjct: 361 YLILGMLCGVVSVAVTRLVAWFSKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY 420

Query: 459 WGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 518
           WGFTNVEEILHTGNRPSAPG+WLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA
Sbjct: 421 WGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 480

Query: 519 VFGGSAVEIINIAIPGNAAVA 537
           VFGGSAVE+IN AIPGNAAVA
Sbjct: 481 VFGGSAVEVINAAIPGNAAVA 501

BLAST of Sgr022077 vs. TAIR 10
Match: AT1G55620.2 (chloride channel F )

HSP 1 Score: 674.9 bits (1740), Expect = 5.7e-194
Identity = 371/504 (73.61%), Postives = 409/504 (81.15%), Query Frame = 0

Query: 42  GEFSDQNRLLRSTEDH-----NGE-DHDLESQDGLGVHPHRNNSGKRGGFLDLFQHLNRG 101
           GE+++   LLRST+        GE D D+ESQ           SG  GG  DLF+H++R 
Sbjct: 7   GEYNEDRHLLRSTDGDEVGIGGGEGDLDVESQS------PAIRSG-AGGVRDLFKHIDRR 66

Query: 102 HSFSGRRLSYKRLDMDNHSVS---YTPSSVNIIGRSRAASTSSDRHNNFHSLPHHAPTAT 161
            S SGRRLS+KR  M+N  V    + PSS +    S A        +N HS+        
Sbjct: 67  FSLSGRRLSFKR--MENIRVDRERHNPSSSSAF--SAAGEEDGGGISNLHSVDDR--NDE 126

Query: 162 DGETEDNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLR 221
            G  E+ L D+APPEWALLLI CL+G+A G+CVA FN GVHVIHEWAWAGTPNEGAAWLR
Sbjct: 127 YGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAWLR 186

Query: 222 LQRMADTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQ 281
           LQR+ADTWHRILLIPVTGGVIVGMMHGLLEIL+QI+QS++SQRQG D LAG++P +KAIQ
Sbjct: 187 LQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGLDFLAGIYPVIKAIQ 246

Query: 282 AAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAV 341
           AA+TLGTGCSLGPEGPSVDIGKSCANGF LMMENN E+ +IA  AAGAA+GIASGFNAAV
Sbjct: 247 AAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRER-RIALTAAGAASGIASGFNAAV 306

Query: 342 AGSFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAE 401
           AG FFAIETVLRPLRAENSPPFTTAMIILASVISSTVSN LLGTQSAFTVP+YDLKSAAE
Sbjct: 307 AGCFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNALLGTQSAFTVPSYDLKSAAE 366

Query: 402 LPLYLILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPG 461
           LPLYLILGMLCG VSV  +RLV WF K F+FIK++FGLP +VCPALGGLGAGIIALKYPG
Sbjct: 367 LPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALKYPG 426

Query: 462 ILYWGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAA 521
           ILYWGFTNVEEILHTG   SAPGIWLL Q+AAAKVVATALCKGSGLVGGLYAPSLMIGAA
Sbjct: 427 ILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMIGAA 486

Query: 522 VGAVFGGSAVEIINIAIPGNAAVA 537
           VGAVFGGSA EIIN AIPGNAAVA
Sbjct: 487 VGAVFGGSAAEIINRAIPGNAAVA 496

BLAST of Sgr022077 vs. TAIR 10
Match: AT1G55620.1 (chloride channel F )

HSP 1 Score: 503.8 bits (1296), Expect = 1.7e-142
Identity = 260/301 (86.38%), Postives = 278/301 (92.36%), Query Frame = 0

Query: 236 MMHGLLEILNQIKQSSTSQRQGFDLLAGVFPTVKAIQAAITLGTGCSLGPEGPSVDIGKS 295
           MMHGLLEIL+QI+QS++SQRQG D LAG++P +KAIQAA+TLGTGCSLGPEGPSVDIGKS
Sbjct: 1   MMHGLLEILDQIRQSNSSQRQGLDFLAGIYPVIKAIQAAVTLGTGCSLGPEGPSVDIGKS 60

Query: 296 CANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIETVLRPLRAENSPPFT 355
           CANGF LMMENN E+ +IA  AAGAA+GIASGFNAAVAG FFAIETVLRPLRAENSPPFT
Sbjct: 61  CANGFALMMENNRER-RIALTAAGAASGIASGFNAAVAGCFFAIETVLRPLRAENSPPFT 120

Query: 356 TAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLYLILGMLCGVVSVAVTRLVA 415
           TAMIILASVISSTVSN LLGTQSAFTVP+YDLKSAAELPLYLILGMLCG VSV  +RLV 
Sbjct: 121 TAMIILASVISSTVSNALLGTQSAFTVPSYDLKSAAELPLYLILGMLCGAVSVVFSRLVT 180

Query: 416 WFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWGFTNVEEILHTGNRPSAPG 475
           WF K F+FIK++FGLP +VCPALGGLGAGIIALKYPGILYWGFTNVEEILHTG   SAPG
Sbjct: 181 WFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALKYPGILYWGFTNVEEILHTGKSASAPG 240

Query: 476 IWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSAVEIINIAIPGNAAV 535
           IWLL Q+AAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSA EIIN AIPGNAAV
Sbjct: 241 IWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSAAEIINRAIPGNAAV 300

Query: 536 A 537
           A
Sbjct: 301 A 300

BLAST of Sgr022077 vs. TAIR 10
Match: AT4G35440.1 (chloride channel E )

HSP 1 Score: 297.4 bits (760), Expect = 2.5e-80
Identity = 171/391 (43.73%), Postives = 242/391 (61.89%), Query Frame = 0

Query: 149 PTATDGETEDNLD-DTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEG 208
           P A     +D +  D  P +   +  ACL+G+ TG+ V  FN  VH++ +++W G P+ G
Sbjct: 54  PAAKQETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRG 113

Query: 209 AAWLRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVF-P 268
           A+WLR   +   W R++L+P  GG++V +++ L E      +S+       D +  V  P
Sbjct: 114 ASWLREAPIGSNWLRVILVPTIGGLVVSILNQLRE---SAGKSTGDSHSSLDRVKAVLRP 173

Query: 269 TVKAIQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIAS 328
            +K + A +TLGTG SLGPEGPSV+IG S A G   +  N S +   + +AAG+AAGI+S
Sbjct: 174 FLKTVAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLF-NKSPQTGFSLLAAGSAAGISS 233

Query: 329 GFNAAVAGSFFAIETVLRPLRAENSP---PFTTAMIILASVISSTVSNVLLGTQSAFTVP 388
           GFNAAVAG FFA+E+VL P  + +S    P TT+M+IL++V +S VS + LG++ AF VP
Sbjct: 234 GFNAAVAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVP 293

Query: 389 TYDLKSAAELPLYLILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGA 448
            YD +S  ELPLYL+LG LCG+VS+A++R  +      + + +  G+P  V P +GGL  
Sbjct: 294 DYDFRSPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSV 353

Query: 449 GIIALKYPGILYWGFTNVEEILHTGNRPSAPGIW--LLTQIAAAKVVATALCKGSGLVGG 508
           GIIAL YP +LYWGF NV+ +L    RP   G+   LL Q+ A K+ ATA C+ SGLVGG
Sbjct: 354 GIIALVYPEVLYWGFQNVDILLE--KRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGG 413

Query: 509 LYAPSLMIGAAVGAVFGGSAVEIINIAIPGN 533
            YAPSL IG A G  +G    + I +A+  N
Sbjct: 414 YYAPSLFIGGAAGMAYG----KFIGLALAQN 434

BLAST of Sgr022077 vs. TAIR 10
Match: AT4G35440.2 (chloride channel E )

HSP 1 Score: 297.4 bits (760), Expect = 2.5e-80
Identity = 171/391 (43.73%), Postives = 242/391 (61.89%), Query Frame = 0

Query: 149 PTATDGETEDNLD-DTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEG 208
           P A     +D +  D  P +   +  ACL+G+ TG+ V  FN  VH++ +++W G P+ G
Sbjct: 54  PAAKQETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRG 113

Query: 209 AAWLRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILNQIKQSSTSQRQGFDLLAGVF-P 268
           A+WLR   +   W R++L+P  GG++V +++ L E      +S+       D +  V  P
Sbjct: 114 ASWLREAPIGSNWLRVILVPTIGGLVVSILNQLRE---SAGKSTGDSHSSLDRVKAVLRP 173

Query: 269 TVKAIQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIAS 328
            +K + A +TLGTG SLGPEGPSV+IG S A G   +  N S +   + +AAG+AAGI+S
Sbjct: 174 FLKTVAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLF-NKSPQTGFSLLAAGSAAGISS 233

Query: 329 GFNAAVAGSFFAIETVLRPLRAENSP---PFTTAMIILASVISSTVSNVLLGTQSAFTVP 388
           GFNAAVAG FFA+E+VL P  + +S    P TT+M+IL++V +S VS + LG++ AF VP
Sbjct: 234 GFNAAVAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVP 293

Query: 389 TYDLKSAAELPLYLILGMLCGVVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGA 448
            YD +S  ELPLYL+LG LCG+VS+A++R  +      + + +  G+P  V P +GGL  
Sbjct: 294 DYDFRSPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSV 353

Query: 449 GIIALKYPGILYWGFTNVEEILHTGNRPSAPGIW--LLTQIAAAKVVATALCKGSGLVGG 508
           GIIAL YP +LYWGF NV+ +L    RP   G+   LL Q+ A K+ ATA C+ SGLVGG
Sbjct: 354 GIIALVYPEVLYWGFQNVDILLE--KRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGG 413

Query: 509 LYAPSLMIGAAVGAVFGGSAVEIINIAIPGN 533
            YAPSL IG A G  +G    + I +A+  N
Sbjct: 414 YYAPSLFIGGAAGMAYG----KFIGLALAQN 434