Sgr021998 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr021998
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	tig00153870: 507660 .. 509574 (+)
RNA-Seq Expression	Sgr021998
Synteny	Sgr021998

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTTGATCCTGCCTTCGTCCAACCCATCGAGCACAGGCCGAAGCTCGAACCCATCGACCCCGTCTACGAAGTCCCGACCATAGACCTCTCCGTCTCCGACTCTCGCACTGCCCAACAGCTCGTCTCGGAAATAGCCTCTGCCTGTGAGAAGTGGGGTTTCTTCCAGGTAATCAACCATGGAGTTCCCGCCGAGTTGCTCCCTGGAATTGAGAAAGCAGCGAAGTCGTTTTTCGATCAGACAGTGGAGGACAAGAGGAAGGTGAAGAGAGATGCAGCCAATGCTATGGGGTTCGACGATGGTGAGAATACGAAAAACGTCCGGGATTGGAAGGAGGTGTTCGATTTCATGATTCAGGACCGGACGTTGATTCCGGCATCGCACGAGCCAGATGACGCGAAATTGAGGGTATTGTCTAATCAATGGCCCCAGTATCCCCCTGGGTTTAGGTACTTTCATTTCTGTCGAATTTGTTCCTTGTCCTAAGCATATACTGAACAGATTCCAGATTCCAGATTCCAGATTCCTCGCCTGTTTTAGTTCCCATGCAAAAATCTAGTTTATGTTTCTTCACATCATATGTTGGATTACTTGAAAGAGAATTAAGGGAGTTGTTATTGGGTTAGAAGAATTGGCACACCAATTGTTCGACTAAATTCCCCTGTGAAACTTTGGGCTTGCATTTCTTCATCTTCTGCTTGAAAAACTTATTATTGTTGGCTCATGGTTTGTGGCAAATGGCGTATTGGTTTTCTTGAACAGGGAAACTTGCGAGGAGTATGCAAGAGAGTTGGAATTGCTAGCACATAAGCTACTGGGGTTAATTTGTTTGACTTTAGGTTTGTCTGCTGCCGATGGATTGAAGGGCTATTTCAAAGATGAGCAAACGAGCGTGGTGAGGTTGAATCACTATCCTCCATGCCAATTTCCCGATCTCGTCCTTGGCGTTGGCCGTCACAAGGATGTGGGTGCCTTGACAATCCTCGCTCAGGATGGTGTTGGAGGGTTGCAAGTGAAGAGAAAATCAGATGGAGAATGGGTTCCTGTGAAGCCAATCCCAAATGCATATATTGTTAACATTGGTGATATTGTTCAAGTATGTTTCCTTTCTACGTATTGACATACAATATACATTTTATGTACGTAACACATACATTTGCATAATCTTCTTTGACCATGGTTATCTGAAAATGATTAGATTTTAAGAAGTGGAAATGACCTCTGAAACTTGTGTGTGAGTAGCATTGATTAAGAAAGGATAGCTTGGTCTAGAGAGTTGGTTTTCTGAAGGATGGAGGTGTCAAGGGGTTGGCTGTACCCGAAGTGCTCACTGCTCTTGTACTGTGGAAAGAAAACATGTCCATTGAGTTCAATAAAACCTTCCAAAGATAGAAGCAGGGATTTCAAACTCTTTTTCCCTCTGTATCCAGCTCTTATCATTAGACTGGTTGTGTGACATAAAACTTCCTTAGTCTCTCCAAGAGCTAATACCTGAGAACCGTGATTCGATAACCAGAACTCTTCGGCCACCAATTCCAACTGAAACATGTTTCCTGCAATTCCTTAGCCTTTATGTGGAACACAGGATTGTTTGGCGGGGTATATAGATTTTGCTTGTGATGAATGTGGTCTGGATAAGGTATGATCCAAATTCATGATGTATGTGCAGGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGGGTGGTGGTGAATACTCAGAAGGAAAGGTTTTCCTTTGTATTCTTCTTCTTTCCAGCGCACCATATGATGGTGAAGCCATTGGAGGAGCTTGTAAATGAGCAGAACCCCCCAAAATACAGAGAGTACAACTGGGGAAAGTTCTTTTGCACCCAAAACCGCAGTGCTTTCAAGAAGCAAAAAGGCAAAAGCAGCCAAATTGATTGA

mRNA sequence

ATGGCCTTTGATCCTGCCTTCGTCCAACCCATCGAGCACAGGCCGAAGCTCGAACCCATCGACCCCGTCTACGAAGTCCCGACCATAGACCTCTCCGTCTCCGACTCTCGCACTGCCCAACAGCTCGTCTCGGAAATAGCCTCTGCCTGTGAGAAGTGGGGTTTCTTCCAGGTAATCAACCATGGAGTTCCCGCCGAGTTGCTCCCTGGAATTGAGAAAGCAGCGAAGTCGTTTTTCGATCAGACAGTGGAGGACAAGAGGAAGGTGAAGAGAGATGCAGCCAATGCTATGGGGTTCGACGATGGTGAGAATACGAAAAACGTCCGGGATTGGAAGGAGGTGTTCGATTTCATGATTCAGGACCGGACGTTGATTCCGGCATCGCACGAGCCAGATGACGCGAAATTGAGGGTATTGTCTAATCAATGGCCCCAGTATCCCCCTGGGTTTAGGGAAACTTGCGAGGAGTATGCAAGAGAGTTGGAATTGCTAGCACATAAGCTACTGGGGTTAATTTGTTTGACTTTAGGTTTGTCTGCTGCCGATGGATTGAAGGGCTATTTCAAAGATGAGCAAACGAGCGTGGTGAGGTTGAATCACTATCCTCCATGCCAATTTCCCGATCTCGTCCTTGGCGTTGGCCGTCACAAGGATGTGGGTGCCTTGACAATCCTCGCTCAGGATGGTGTTGGAGGGTTGCAAGTGAAGAGAAAATCAGATGGAGAATGGGTTCCTGTGAAGCCAATCCCAAATGCATATATTGTTAACATTGGTGATATTGTTCAAGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGGGTGGTGGTGAATACTCAGAAGGAAAGGTTTTCCTTTGTATTCTTCTTCTTTCCAGCGCACCATATGATGGTGAAGCCATTGGAGGAGCTTGTAAATGAGCAGAACCCCCCAAAATACAGAGAGTACAACTGGGGAAAGTTCTTTTGCACCCAAAACCGCAGTGCTTTCAAGAAGCAAAAAGGCAAAAGCAGCCAAATTGATTGA

Coding sequence (CDS)

ATGGCCTTTGATCCTGCCTTCGTCCAACCCATCGAGCACAGGCCGAAGCTCGAACCCATCGACCCCGTCTACGAAGTCCCGACCATAGACCTCTCCGTCTCCGACTCTCGCACTGCCCAACAGCTCGTCTCGGAAATAGCCTCTGCCTGTGAGAAGTGGGGTTTCTTCCAGGTAATCAACCATGGAGTTCCCGCCGAGTTGCTCCCTGGAATTGAGAAAGCAGCGAAGTCGTTTTTCGATCAGACAGTGGAGGACAAGAGGAAGGTGAAGAGAGATGCAGCCAATGCTATGGGGTTCGACGATGGTGAGAATACGAAAAACGTCCGGGATTGGAAGGAGGTGTTCGATTTCATGATTCAGGACCGGACGTTGATTCCGGCATCGCACGAGCCAGATGACGCGAAATTGAGGGTATTGTCTAATCAATGGCCCCAGTATCCCCCTGGGTTTAGGGAAACTTGCGAGGAGTATGCAAGAGAGTTGGAATTGCTAGCACATAAGCTACTGGGGTTAATTTGTTTGACTTTAGGTTTGTCTGCTGCCGATGGATTGAAGGGCTATTTCAAAGATGAGCAAACGAGCGTGGTGAGGTTGAATCACTATCCTCCATGCCAATTTCCCGATCTCGTCCTTGGCGTTGGCCGTCACAAGGATGTGGGTGCCTTGACAATCCTCGCTCAGGATGGTGTTGGAGGGTTGCAAGTGAAGAGAAAATCAGATGGAGAATGGGTTCCTGTGAAGCCAATCCCAAATGCATATATTGTTAACATTGGTGATATTGTTCAAGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGGGTGGTGGTGAATACTCAGAAGGAAAGGTTTTCCTTTGTATTCTTCTTCTTTCCAGCGCACCATATGATGGTGAAGCCATTGGAGGAGCTTGTAAATGAGCAGAACCCCCCAAAATACAGAGAGTACAACTGGGGAAAGTTCTTTTGCACCCAAAACCGCAGTGCTTTCAAGAAGCAAAAAGGCAAAAGCAGCCAAATTGATTGA

Protein sequence

MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVINHGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQDRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSAADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSDGEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVKPLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID

Homology

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BLAST of Sgr021998 vs. NCBI nr
Match: XP_022135969.1 (flavonol synthase/flavanone 3-hydroxylase-like [Momordica charantia])

HSP 1 Score: 620.9 bits (1600), Expect = 6.4e-174
Identity = 294/342 (85.96%), Postives = 317/342 (92.69%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVIN 60
           +AFDPAF+Q IEHRP+LEPID V EVPTIDLSVSDSR+ +QLVSEIASACEKWGFFQVIN
Sbjct: 5   LAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFFQVIN 64

Query: 61  HGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQ 120
           HGVP EL   ++KAAKSFFDQTVE+KR VKRDAANAMGF D ENTKNVRDWKEVFDF+I+
Sbjct: 65  HGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFDFLIR 124

Query: 121 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 180
           DRTLIPASHEPDD KLRVLSNQWPQYPPGFRETCEEYARELELLA KLLGLICL+LGL  
Sbjct: 125 DRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLPG 184

Query: 181 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSD 240
           A+GL+GYFKD+QTSVVRLNHYPPC  P+LVLGVG HKD GALTILAQDGVGGLQVKRKSD
Sbjct: 185 AEGLRGYFKDDQTSVVRLNHYPPCPSPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKSD 244

Query: 241 GEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVK 300
           GEW+PVKPIPNAYIVNIGDIVQVWSNDKYESVEHR VVNT+KERFSF FFFFPAHHMMVK
Sbjct: 245 GEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHMMVK 304

Query: 301 PLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           PLEELVN+QNP KYREYNWGKFFCT+NRS ++KQK ++ QID
Sbjct: 305 PLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQID 346

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BLAST of Sgr021998 vs. NCBI nr
Match: XP_038888870.1 (flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida])

HSP 1 Score: 601.7 bits (1550), Expect = 4.0e-168
Identity = 285/343 (83.09%), Postives = 313/343 (91.25%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPV-YEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVI 60
           +A D  F+QPIEHRPKLEP++P+  EVPTIDLS+S SRT QQLVSEIASAC+ WGFFQVI
Sbjct: 5   LAMDSTFIQPIEHRPKLEPLNPINEEVPTIDLSISLSRTTQQLVSEIASACQNWGFFQVI 64

Query: 61  NHGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMI 120
           NHGVP ELL  I+K AK FFDQTVE+KRKVKRDAANAMGF DGENTKNVRDWKEVFDF++
Sbjct: 65  NHGVPLELLARIKKTAKLFFDQTVEEKRKVKRDAANAMGFHDGENTKNVRDWKEVFDFLV 124

Query: 121 QDRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLS 180
           +D T IPASH+P+D KLRVL NQWPQYPPGFRETCE+YARELELLAHKLLGLI L+LGLS
Sbjct: 125 KDETFIPASHDPNDKKLRVLRNQWPQYPPGFRETCEDYARELELLAHKLLGLISLSLGLS 184

Query: 181 AADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKS 240
            ADGLKGYF DEQTS+VRLNHYPPC  PDLVLGVGRHKD GALTILAQDGVGGLQVKRKS
Sbjct: 185 GADGLKGYFNDEQTSLVRLNHYPPCPSPDLVLGVGRHKDAGALTILAQDGVGGLQVKRKS 244

Query: 241 DGEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMV 300
           D EW+PVKP+PNA+IVNIGD+VQVWSNDKYESVEHRVVVNT+KERFSF FFFFPAHHMMV
Sbjct: 245 DEEWIPVKPVPNAFIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAHHMMV 304

Query: 301 KPLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           KPLEELVNEQNPPKYREYN+GKFF ++NRS F+KQK ++ QID
Sbjct: 305 KPLEELVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENIQID 347

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BLAST of Sgr021998 vs. NCBI nr
Match: KAG7022434.1 (Protein DMR6-LIKE OXYGENASE 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 596.7 bits (1537), Expect = 1.3e-166
Identity = 277/342 (80.99%), Postives = 313/342 (91.52%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVIN 60
           +AFD +F+QPI+HRPKLEP+DP+ EVPTIDLS+SDSR+ +QLVS+IASACE WGFFQVIN
Sbjct: 5   LAFDASFIQPIQHRPKLEPVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFFQVIN 64

Query: 61  HGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQ 120
           HGVP EL+  +E+  K FFDQ V++KRKVKRDAANAMG+ DGENTKNVRDWKEVFDF+++
Sbjct: 65  HGVPMELVARMEEVTKLFFDQGVDEKRKVKRDAANAMGYHDGENTKNVRDWKEVFDFLVK 124

Query: 121 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 180
           DRTL+PASHEPDD  LRVL+NQWPQYPPGFRETCEEYA+ELELLA+KLLGLICL+LGLS 
Sbjct: 125 DRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLSLGLSV 184

Query: 181 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSD 240
           AD LKGYF+DEQ S+VRLN YPPC  PDLVLGVGRHKD GALTILAQDGVGGLQV+RKSD
Sbjct: 185 ADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVRRKSD 244

Query: 241 GEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVK 300
           GEW+PVKPI NAYIVNIGDIVQVWSNDKYESVEHRVVVNT+KERFS  FFFFPAHH MVK
Sbjct: 245 GEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHHTMVK 304

Query: 301 PLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           PLEE+VNEQNPPKYREYN+GKFF ++NRS F+KQK ++ QID
Sbjct: 305 PLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQID 346

[Close]

BLAST of Sgr021998 vs. NCBI nr
Match: XP_022931615.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 595.5 bits (1534), Expect = 2.9e-166
Identity = 277/342 (80.99%), Postives = 312/342 (91.23%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVIN 60
           +AFD +F+QPI+HRPKLEP+DP+ EVPTIDLS+SDSR+ +QLVS+IASACE WGFFQVIN
Sbjct: 5   LAFDASFIQPIQHRPKLEPVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFFQVIN 64

Query: 61  HGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQ 120
           HGVP EL+  +E+  K FFDQ VE+KRKVKRDAANAMG+ DGENTKNVRDWKEVFDF+++
Sbjct: 65  HGVPMELVARMEEVTKLFFDQGVEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFDFLVK 124

Query: 121 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 180
           DRTL+PASHEPDD  LRVL+NQWPQYPPGFRETCEEYA+ELELLA+KLLGLICL+LGL  
Sbjct: 125 DRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLSLGLPI 184

Query: 181 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSD 240
           AD LKGYF+DEQ S+VRLN YPPC  PDLVLGVGRHKD GALTILAQDGVGGLQV+RKSD
Sbjct: 185 ADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVRRKSD 244

Query: 241 GEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVK 300
           GEW+PVKPI NAYIVNIGDIVQVWSNDKYESVEHRVVVNT+KERFS  FFFFPAHH MVK
Sbjct: 245 GEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHHTMVK 304

Query: 301 PLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           PLEE+VNEQNPPKYREYN+GKFF ++NRS F+KQK ++ QID
Sbjct: 305 PLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQID 346

[Close]

BLAST of Sgr021998 vs. NCBI nr
Match: XP_022988832.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 591.3 bits (1523), Expect = 5.5e-165
Identity = 277/342 (80.99%), Postives = 310/342 (90.64%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVIN 60
           +AFD +F+QPIEHRPKLE +DP+ EVPTIDLS+SDSR+ +QLVS+IASACE WGFFQVIN
Sbjct: 5   LAFDASFIQPIEHRPKLEHVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFFQVIN 64

Query: 61  HGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQ 120
           HGVP ELL  +EK AK FFDQ +E+KRKVKRDAANAMG+ DGENTKNVRDWKEVFDF+++
Sbjct: 65  HGVPMELLARMEKVAKLFFDQGIEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFDFLVR 124

Query: 121 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 180
           DRTL+PASHEPD   LRVL+NQWPQYPP FRETCEEYA+ELELLA+KLLGLICL+LGL  
Sbjct: 125 DRTLVPASHEPDGTSLRVLTNQWPQYPPEFRETCEEYAKELELLAYKLLGLICLSLGLPI 184

Query: 181 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSD 240
           AD LKGYF+DEQ S+VRLN YPPC  PDLVLGVGRHKD GALTILAQDGVGGLQV+RKSD
Sbjct: 185 ADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVRRKSD 244

Query: 241 GEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVK 300
           GEW+PVKPI NAYIVNIGDIVQVWSNDKYESVEHRVVVNT+KERFS  FFFFPAHH MVK
Sbjct: 245 GEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHHTMVK 304

Query: 301 PLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           PLEE+VNEQNPPKYREYN+GKFF ++NRS F+KQK ++ QID
Sbjct: 305 PLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQID 346

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BLAST of Sgr021998 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.9e-49
Identity = 113/315 (35.87%), Postives = 167/315 (53.02%), Query Frame = 0


Query: 7   FVQPIEHRP-KLEPIDPVYEVPTIDLS---VSDSRTAQQLVSEIASACEKWGFFQVINHG 66
           +++P   RP   E       +P IDL      +  +   +++ I+ AC  WGFFQV+NHG
Sbjct: 42  YIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHG 101

Query: 67  VPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDD--GENTKNVRDWKEVFDFMIQ 126
           V  EL+    +  + FF   V  K           G+    G       DW + + F+  
Sbjct: 102 VKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYY-FL-- 161

Query: 127 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 186
              L+P  H   D       N+WP +PP  RE  +EY  EL  L+ +++ ++   LGL  
Sbjct: 162 --HLLP--HHLKD------FNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKE 221

Query: 187 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTI-LAQDGVGGLQVKRKS 246
               + +  +   + +R+N+YP C  P+L LG+  H D G +TI L  D V GLQV++  
Sbjct: 222 DKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRK-- 281

Query: 247 DGEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMV 306
           D  W+ VKP P+A+IVNIGD +Q+ SN  Y+SVEHRV+VN+ KER S  FF+ P   + +
Sbjct: 282 DDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPI 341

Query: 307 KPLEELVNEQNPPKY 315
           +PL+ELV+  NPP Y
Sbjct: 342 QPLQELVSTHNPPLY 341

[Close]

BLAST of Sgr021998 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.7e-49
Identity = 115/326 (35.28%), Postives = 174/326 (53.37%), Query Frame = 0


Query: 7   FVQPIEHRPKLEPI-DPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVINHGVPA 66
           F++P + +P       P  E+PTIDL   D     +LV  IA A  +WG FQV NHG+P+
Sbjct: 23  FIRPEKEQPASTTYHGPAPEIPTIDL---DDPVQDRLVRSIAEASREWGIFQVTNHGIPS 82

Query: 67  ELLPGIEKAAKSFFDQTVEDKRKVKR--DAANAMGFDDGENTKNVRDWKEVFDFMIQDRT 126
           +L+  ++   K FF+   E+K    R  DA +  G+   +  K V   K   D +   R 
Sbjct: 83  DLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGT-KLQKEVEGKKSWVDHLFH-RV 142

Query: 127 LIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSAADG 186
             P+S         +    WP+ PP +R   EEYA+ +  +  KL   + L LG+     
Sbjct: 143 WPPSS---------INYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLGLGVEGGVL 202

Query: 187 LKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSDGEW 246
            +    D+   ++++N+YPPC  PDL LGV  H D+ ALT+L  + V GLQV +  D  W
Sbjct: 203 KEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFK--DDRW 262

Query: 247 VPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVKPLE 306
           +  K IPNA +++IGD +++ SN KY++V HR  VN  K R S+  F  P    +V PL 
Sbjct: 263 IDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADTVVGPLP 322

Query: 307 ELVNEQNPPKYREYNWGKF-FCTQNR 329
           +LV+++NPPKY+   +  + +C  N+
Sbjct: 323 QLVDDENPPKYKAKKFKDYSYCKLNK 332

[Close]

BLAST of Sgr021998 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.5e-48
Identity = 127/337 (37.69%), Postives = 177/337 (52.52%), Query Frame = 0


Query: 7   FVQPIEHRPKLEPIDPVYE-VPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVINHGVPA 66
           +V+PI  RP L  ++   + +P IDL          +V ++ASAC  +GFFQ+ NHGVP 
Sbjct: 24  YVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNHGVPD 83

Query: 67  ELLPGIEKAAKSFFDQTVEDKRKVKRDAAN-------AMGFDDG-ENTKNVRDWKEVFDF 126
             +  ++  A+ FF Q   +  +VK  +A+       +  F+ G +   N RD+  +  F
Sbjct: 84  TTVNKMQTVAREFFHQ--PESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLHCF 143

Query: 127 MIQDRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLG 186
            I+D                    +WP  P  FRE   EYA  +  L  +LL  I  +LG
Sbjct: 144 PIED-----------------FIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLG 203

Query: 187 LSAADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKR 246
           L  +D +      +    +  N+YPPC  P+L  G+  HKD   +T+L QD V GLQV +
Sbjct: 204 LE-SDHISNIL-GKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFK 263

Query: 247 KSDGEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHM 306
             D +WV V PIPN +IVNIGD +QV SNDKY+SV HR VVNT+ ER S   F+FP+   
Sbjct: 264 --DDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDA 323

Query: 307 MVKPLEELVNEQNP-PKYREY----NWGKFFCTQNRS 330
           ++ P  ELVNEQ+    YR Y     W KF+   NRS
Sbjct: 324 VIGPAHELVNEQDSLAIYRTYPFVEYWDKFW---NRS 334

[Close]

BLAST of Sgr021998 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.5e-48
Identity = 113/326 (34.66%), Postives = 172/326 (52.76%), Query Frame = 0


Query: 7   FVQPIEHRPKLEPIDPVYE-VPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVINHGVPA 66
           +V+P+  RPK+  +    + +P IDL          ++++ A AC   GFFQ+ NHGVP 
Sbjct: 22  YVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNHGVPE 81

Query: 67  ELLPGIEKAAKSFFDQTVEDKRK-----VKRDAANAMGFD-DGENTKNVRDWKEVFDFMI 126
           E +  +  AA+ FF Q+  ++ K      K+    +  F+   E   N RD+  +  + I
Sbjct: 82  ETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPI 141

Query: 127 QDRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLS 186
           +D                   N+WP  P  FRE   EYA  +  L   LL  I  +LGL 
Sbjct: 142 ED-----------------FINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGL- 201

Query: 187 AADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKS 246
           A D +      +    + +N+YP C  P+L  G+  HKD   +T+L QD V GLQV +  
Sbjct: 202 AKDRVSNTI-GKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFK-- 261

Query: 247 DGEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMV 306
           DG+W+ V P+PN +IVN+GD +QV SN+KY+SV HR VVN+  ER S   F+ P+   ++
Sbjct: 262 DGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVI 321

Query: 307 KPLEELVNEQ--NPPKYREYNWGKFF 324
            P +EL+NE+  +P  YR + + ++F
Sbjct: 322 SPAQELINEEEDSPAIYRNFTYAEYF 326

[Close]

BLAST of Sgr021998 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.5e-48
Identity = 113/307 (36.81%), Postives = 168/307 (54.72%), Query Frame = 0


Query: 23  VYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVINHGVPAELLPGIEKAAKSFFDQT 82
           V EVP IDLS SD +    LVSE   A ++WG FQV+NHG+P E++  +++  K FF+  
Sbjct: 40  VLEVPVIDLSDSDEKKIVGLVSE---ASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELP 99

Query: 83  VEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQDRTLIPASHEPDDAKLRVLSNQ 142
            E+K  + +   +      G   +   D K+ +   +  +   P++         +    
Sbjct: 100 QEEKELIAKPEGSQSIEGYGTRLQKEVDGKKGWVDHLFHKIWPPSA---------INYQF 159

Query: 143 WPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSAADGLKGYFKDEQTSVVRLNHYP 202
           WP+ PP +RE  EEYA+ L+L+   L   + L L L       G   D+   ++++N+YP
Sbjct: 160 WPKNPPAYREANEEYAKRLQLVVDNLFKYLSLGLDLEPNSFKDGAGGDDLVYLMKINYYP 219

Query: 203 PCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSDGEWVPVKPIPNAYIVNIGDIVQ 262
           PC  PDL LGV  H D+ A+T+L  + V GLQV +  DG W   K IPNA IV+IGD V+
Sbjct: 220 PCPRPDLALGVA-HTDMSAITVLVPNEVPGLQVYK--DGHWYDCKYIPNALIVHIGDQVE 279

Query: 263 VWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVKPLEELVNEQNPPKYREYNWGKF 322
           + SN KY+SV HR  VN +K R S+  F  P     V P+ +LVNE+NP K++   +  +
Sbjct: 280 IMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDHEVGPIPKLVNEENPAKFKTKKYKDY 331

Query: 323 -FCTQNR 329
            +C  N+
Sbjct: 340 AYCKLNK 331

[Close]

BLAST of Sgr021998 vs. ExPASy TrEMBL
Match: A0A6J1C2Z2 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111007793 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.1e-174
Identity = 294/342 (85.96%), Postives = 317/342 (92.69%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVIN 60
           +AFDPAF+Q IEHRP+LEPID V EVPTIDLSVSDSR+ +QLVSEIASACEKWGFFQVIN
Sbjct: 5   LAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFFQVIN 64

Query: 61  HGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQ 120
           HGVP EL   ++KAAKSFFDQTVE+KR VKRDAANAMGF D ENTKNVRDWKEVFDF+I+
Sbjct: 65  HGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFDFLIR 124

Query: 121 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 180
           DRTLIPASHEPDD KLRVLSNQWPQYPPGFRETCEEYARELELLA KLLGLICL+LGL  
Sbjct: 125 DRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLPG 184

Query: 181 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSD 240
           A+GL+GYFKD+QTSVVRLNHYPPC  P+LVLGVG HKD GALTILAQDGVGGLQVKRKSD
Sbjct: 185 AEGLRGYFKDDQTSVVRLNHYPPCPSPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKSD 244

Query: 241 GEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVK 300
           GEW+PVKPIPNAYIVNIGDIVQVWSNDKYESVEHR VVNT+KERFSF FFFFPAHHMMVK
Sbjct: 245 GEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHMMVK 304

Query: 301 PLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           PLEELVN+QNP KYREYNWGKFFCT+NRS ++KQK ++ QID
Sbjct: 305 PLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQID 346

[Close]

BLAST of Sgr021998 vs. ExPASy TrEMBL
Match: A0A6J1EZZ4 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111437780 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.4e-166
Identity = 277/342 (80.99%), Postives = 312/342 (91.23%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVIN 60
           +AFD +F+QPI+HRPKLEP+DP+ EVPTIDLS+SDSR+ +QLVS+IASACE WGFFQVIN
Sbjct: 5   LAFDASFIQPIQHRPKLEPVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFFQVIN 64

Query: 61  HGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQ 120
           HGVP EL+  +E+  K FFDQ VE+KRKVKRDAANAMG+ DGENTKNVRDWKEVFDF+++
Sbjct: 65  HGVPMELVARMEEVTKLFFDQGVEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFDFLVK 124

Query: 121 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 180
           DRTL+PASHEPDD  LRVL+NQWPQYPPGFRETCEEYA+ELELLA+KLLGLICL+LGL  
Sbjct: 125 DRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLSLGLPI 184

Query: 181 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSD 240
           AD LKGYF+DEQ S+VRLN YPPC  PDLVLGVGRHKD GALTILAQDGVGGLQV+RKSD
Sbjct: 185 ADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVRRKSD 244

Query: 241 GEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVK 300
           GEW+PVKPI NAYIVNIGDIVQVWSNDKYESVEHRVVVNT+KERFS  FFFFPAHH MVK
Sbjct: 245 GEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHHTMVK 304

Query: 301 PLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           PLEE+VNEQNPPKYREYN+GKFF ++NRS F+KQK ++ QID
Sbjct: 305 PLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQID 346

[Close]

BLAST of Sgr021998 vs. ExPASy TrEMBL
Match: A0A6J1JE50 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111486062 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.6e-165
Identity = 277/342 (80.99%), Postives = 310/342 (90.64%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVIN 60
           +AFD +F+QPIEHRPKLE +DP+ EVPTIDLS+SDSR+ +QLVS+IASACE WGFFQVIN
Sbjct: 5   LAFDASFIQPIEHRPKLEHVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFFQVIN 64

Query: 61  HGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQ 120
           HGVP ELL  +EK AK FFDQ +E+KRKVKRDAANAMG+ DGENTKNVRDWKEVFDF+++
Sbjct: 65  HGVPMELLARMEKVAKLFFDQGIEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFDFLVR 124

Query: 121 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 180
           DRTL+PASHEPD   LRVL+NQWPQYPP FRETCEEYA+ELELLA+KLLGLICL+LGL  
Sbjct: 125 DRTLVPASHEPDGTSLRVLTNQWPQYPPEFRETCEEYAKELELLAYKLLGLICLSLGLPI 184

Query: 181 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSD 240
           AD LKGYF+DEQ S+VRLN YPPC  PDLVLGVGRHKD GALTILAQDGVGGLQV+RKSD
Sbjct: 185 ADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVRRKSD 244

Query: 241 GEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVK 300
           GEW+PVKPI NAYIVNIGDIVQVWSNDKYESVEHRVVVNT+KERFS  FFFFPAHH MVK
Sbjct: 245 GEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHHTMVK 304

Query: 301 PLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           PLEE+VNEQNPPKYREYN+GKFF ++NRS F+KQK ++ QID
Sbjct: 305 PLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQID 346

[Close]

BLAST of Sgr021998 vs. ExPASy TrEMBL
Match: A0A6J1C2J5 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111007794 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.5e-165
Identity = 282/341 (82.70%), Postives = 308/341 (90.32%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVIN 60
           +AFDPAF+QPIEHRP+LEPID V EVPTIDLSVSDSR+ +QLVSEI+SACEKWGFFQVIN
Sbjct: 5   LAFDPAFIQPIEHRPELEPIDSVDEVPTIDLSVSDSRSIEQLVSEISSACEKWGFFQVIN 64

Query: 61  HGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQ 120
           HGVP +L   +EKAAKSFFDQTVE+KRKVKRDAANAMGF D ENTKNVRDWKEVFDF+I+
Sbjct: 65  HGVPVDLHARLEKAAKSFFDQTVEEKRKVKRDAANAMGFHDAENTKNVRDWKEVFDFLIR 124

Query: 121 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 180
            RTLIPASHEPDD KLRVL+NQWPQYPPGFRETCEEYA+ELELLAHKLLGL+ L+LGL  
Sbjct: 125 GRTLIPASHEPDDTKLRVLANQWPQYPPGFRETCEEYAKELELLAHKLLGLVSLSLGL-P 184

Query: 181 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSD 240
           AD LK YFKD+Q ++VRLNHYP C FP+LVLGVGRHKD  ALTILAQ  VGGLQVKRKSD
Sbjct: 185 ADRLKEYFKDDQLTIVRLNHYPLCPFPELVLGVGRHKDPSALTILAQGAVGGLQVKRKSD 244

Query: 241 GEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVK 300
           GEW+PVKP PNAYIVNIGDIVQVWSNDKYESVEHR VVNT+KERFSF F  +P HHMMVK
Sbjct: 245 GEWIPVKPNPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFLLYPPHHMMVK 304

Query: 301 PLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQI 342
           PLEELVNEQNPPKYREYNWGKF CT+NRS FKKQ+ ++ QI
Sbjct: 305 PLEELVNEQNPPKYREYNWGKFLCTRNRSDFKKQQVENIQI 344

[Close]

BLAST of Sgr021998 vs. ExPASy TrEMBL
Match: A0A6J1C6B7 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111007792 PE=3 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.7e-159
Identity = 269/341 (78.89%), Postives = 299/341 (87.68%), Query Frame = 0


Query: 1   MAFDPAFVQPIEHRPKLEPIDPVYEVPTIDLSVSDSRTAQQLVSEIASACEKWGFFQVIN 60
           +AF PAFV+ IEHRPKLEPID V EVPTIDLSVS+SR+ +QLVSEIASACEKWG FQVIN
Sbjct: 5   LAFHPAFVRDIEHRPKLEPIDTVDEVPTIDLSVSESRSIEQLVSEIASACEKWGSFQVIN 64

Query: 61  HGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQ 120
           HGVP EL+  + KAAK FFDQTVE+KRKVKRD+   +GF DGENT+NVRDWKEVFDF++Q
Sbjct: 65  HGVPVELIVRLAKAAKLFFDQTVEEKRKVKRDSETGLGFYDGENTENVRDWKEVFDFLVQ 124

Query: 121 DRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSA 180
           DR   PASHEPDD KLRVLSNQWPQYPP FRETCEEYARELELLAH LLGLICL+LGL  
Sbjct: 125 DRAWTPASHEPDDTKLRVLSNQWPQYPPWFRETCEEYARELELLAHNLLGLICLSLGLPC 184

Query: 181 ADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSD 240
           A+GL+GYF+DE+ S VRLNHYPPC  PDLVLG+  HKD GALTILAQD VGGLQVKRKSD
Sbjct: 185 AEGLRGYFQDEKMSTVRLNHYPPCPSPDLVLGIDPHKDPGALTILAQDDVGGLQVKRKSD 244

Query: 241 GEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVK 300
           GEW+PVKPIPNAYIVNIG+I+QVWSNDKYESVEHR VVN++KERFSF F F PAHHMMVK
Sbjct: 245 GEWIPVKPIPNAYIVNIGEIIQVWSNDKYESVEHRAVVNSEKERFSFPFCFLPAHHMMVK 304

Query: 301 PLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQI 342
           P EELVNEQNPPKYREYNWGK+FC + RS FK+Q+ +  Q+
Sbjct: 305 PWEELVNEQNPPKYREYNWGKYFCMRKRSHFKEQEVEDIQV 345

[Close]

BLAST of Sgr021998 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 426.8 bits (1096), Expect = 1.7e-119
Identity = 205/346 (59.25%), Postives = 261/346 (75.43%), Query Frame = 0


Query: 4   DPAFVQPIEHRPKLEPI---DPVY--EVPTIDLSVSDS--RTAQQLVSEIASACEKWGFF 63
           D AF+Q  EHRP        D ++  E+PTIDLS  +        +  EIA AC++WGFF
Sbjct: 5   DEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFF 64

Query: 64  QVINHGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFD 123
           QVINHG+P+ L   +EK A  FF+ T E+KRKVKRD  N MG+ D E+TKNVRDWKE+FD
Sbjct: 65  QVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFD 124

Query: 124 FMIQDRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTL 183
           F +QD T++PAS EP+D +LR L+NQWPQ P  FRE C+EYARE+E LA +LL L+ ++L
Sbjct: 125 FFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISL 184

Query: 184 GLSAADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVK 243
           GL   D L G+F +EQTS +R NHYPPC  P+L LGVGRHKD GALT+LAQD VGGLQV 
Sbjct: 185 GL-PGDRLTGFF-NEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVS 244

Query: 244 RKSDGEWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHH 303
           R+SDG+W+PVKPI +A I+N+G+ +QVW+ND+Y S EHRVVVNT KERFS  FFFFP+H 
Sbjct: 245 RRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHE 304

Query: 304 MMVKPLEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
             ++PLEEL++E+NPP Y++YNWGKFF ++NRS FKK + ++ QID
Sbjct: 305 ANIEPLEELISEENPPCYKKYNWGKFFVSRNRSDFKKLEVENIQID 348

[Close]

BLAST of Sgr021998 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 389.8 bits (1000), Expect = 2.2e-108
Identity = 186/341 (54.55%), Postives = 243/341 (71.26%), Query Frame = 0


Query: 4   DPAFVQPIEHRPKLEPI-DPVYEVPTIDLS-VSDSRTAQQLVSEIASACEKWGFFQVINH 63
           DP ++Q  EHR     I +   E+P IDLS + D    Q ++SEI  ACEKWGFFQV+NH
Sbjct: 5   DPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLNH 64

Query: 64  GVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQD 123
           GVP++    +EK  K FFD  +E+K KVKRD  N +G+ DGE+TKNV+DWKEVFD   +D
Sbjct: 65  GVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKD 124

Query: 124 RTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSAA 183
             +IP++ +P+D  LR++ N+WPQ P  FRE CE YAR  E LA KLL LI L+LGL   
Sbjct: 125 PMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGL-PK 184

Query: 184 DGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSDG 243
           +    YFK EQ S  R+N YPPC  PDL LGVG HKD   +++LAQD VGGLQV R+SDG
Sbjct: 185 ERFHDYFK-EQMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDG 244

Query: 244 EWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVKP 303
            W P++P+PNA ++NIG+ +++W+NDKY S EHRVVVNT +ER+S  FF  P+H + VKP
Sbjct: 245 VWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKP 304

Query: 304 LEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           LEELV+ +NPPKY+ Y WGKF+ ++NRS F+K + ++ QID
Sbjct: 305 LEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQID 343

[Close]

BLAST of Sgr021998 vs. TAIR 10
Match: AT3G19010.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 355.5 bits (911), Expect = 4.7e-98
Identity = 170/341 (49.85%), Postives = 225/341 (65.98%), Query Frame = 0


Query: 4   DPAFVQPIEHRPKLEPI-DPVYEVPTIDLS-VSDSRTAQQLVSEIASACEKWGFFQVINH 63
           DP ++Q  EHR     I +   E+P IDLS + D    Q ++SEI  ACEKWGFFQV+NH
Sbjct: 5   DPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLNH 64

Query: 64  GVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQD 123
           GVP++    +EK  K FFD  +E+K KVKRD  N +G+ DGE+TKNV+DWKEVFD   +D
Sbjct: 65  GVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKD 124

Query: 124 RTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSAA 183
             +IP++ +P+D  LR++ N+WPQ P  FRE CE YAR  E LA                
Sbjct: 125 PMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLA---------------- 184

Query: 184 DGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSDG 243
                           +N YPPC  PDL LGVG HKD   +++LAQD VGGLQV R+SDG
Sbjct: 185 ----------------INRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDG 244

Query: 244 EWVPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRVVVNTQKERFSFVFFFFPAHHMMVKP 303
            W P++P+PNA ++NIG+ +++W+NDKY S EHRVVVNT +ER+S  FF  P+H + VKP
Sbjct: 245 VWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKP 304

Query: 304 LEELVNEQNPPKYREYNWGKFFCTQNRSAFKKQKGKSSQID 343
           LEELV+ +NPPKY+ Y WGKF+ ++NRS F+K + ++ QID
Sbjct: 305 LEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQID 313

[Close]

BLAST of Sgr021998 vs. TAIR 10
Match: AT3G19000.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 316.6 bits (810), Expect = 2.4e-86
Identity = 155/267 (58.05%), Postives = 196/267 (73.41%), Query Frame = 0


Query: 4   DPAFVQPIEHRPKLEPI---DPVY--EVPTIDLSVSDS--RTAQQLVSEIASACEKWGFF 63
           D AF+Q  EHRP        D ++  E+PTIDLS  +        +  EIA AC++WGFF
Sbjct: 5   DEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFF 64

Query: 64  QVINHGVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFD 123
           QVINHG+P+ L   +EK A  FF+ T E+KRKVKRD  N MG+ D E+TKNVRDWKE+FD
Sbjct: 65  QVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFD 124

Query: 124 FMIQDRTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTL 183
           F +QD T++PAS EP+D +LR L+NQWPQ P  FRE C+EYARE+E LA +LL L+ ++L
Sbjct: 125 FFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISL 184

Query: 184 GLSAADGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVK 243
           GL   D L G+F +EQTS +R NHYPPC  P+L LGVGRHKD GALT+LAQD VGGLQV 
Sbjct: 185 GL-PGDRLTGFF-NEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVS 244

Query: 244 RKSDGEWVPVKPIPNAYIVNIGDIVQV 264
           R+SDG+W+PVKPI +A I+N+G+ +QV
Sbjct: 245 RRSDGQWIPVKPISDALIINMGNCIQV 269

[Close]

BLAST of Sgr021998 vs. TAIR 10
Match: AT3G19010.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 5.4e-78
Identity = 140/262 (53.44%), Postives = 181/262 (69.08%), Query Frame = 0


Query: 4   DPAFVQPIEHRPKLEPI-DPVYEVPTIDLS-VSDSRTAQQLVSEIASACEKWGFFQVINH 63
           DP ++Q  EHR     I +   E+P IDLS + D    Q ++SEI  ACEKWGFFQV+NH
Sbjct: 5   DPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLNH 64

Query: 64  GVPAELLPGIEKAAKSFFDQTVEDKRKVKRDAANAMGFDDGENTKNVRDWKEVFDFMIQD 123
           GVP++    +EK  K FFD  +E+K KVKRD  N +G+ DGE+TKNV+DWKEVFD   +D
Sbjct: 65  GVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKD 124

Query: 124 RTLIPASHEPDDAKLRVLSNQWPQYPPGFRETCEEYARELELLAHKLLGLICLTLGLSAA 183
             +IP++ +P+D  LR++ N+WPQ P  FRE CE YAR  E LA KLL LI L+LGL   
Sbjct: 125 PMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGL-PK 184

Query: 184 DGLKGYFKDEQTSVVRLNHYPPCQFPDLVLGVGRHKDVGALTILAQDGVGGLQVKRKSDG 243
           +    YFK EQ S  R+N YPPC  PDL LGVG HKD   +++LAQD VGGLQV R+SDG
Sbjct: 185 ERFHDYFK-EQMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDG 244

Query: 244 EWVPVKPIPNAYIVNIGDIVQV 264
            W P++P+PNA ++NIG+ ++V
Sbjct: 245 VWFPIRPVPNALVINIGNCMEV 264

The following BLAST results are available for this feature:

BLAST of Sgr021998 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135969.1	6.4e-174	85.96	flavonol synthase/flavanone 3-hydroxylase-like [Momordica charantia]	[more]
XP_038888870.1	4.0e-168	83.09	flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida]	[more]
KAG7022434.1	1.3e-166	80.99	Protein DMR6-LIKE OXYGENASE 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022931615.1	2.9e-166	80.99	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata]	[more]
XP_022988832.1	5.5e-165	80.99	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima]	[more]

BLAST of Sgr021998 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FFF6	3.9e-49	35.87	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]
Q9ZWQ9	6.7e-49	35.28	Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ...	[more]
Q9ZSA8	1.5e-48	37.69	Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1	[more]
Q9ZSA7	1.5e-48	34.66	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
Q9M547	1.5e-48	36.81	Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian...	[more]

BLAST of Sgr021998 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2Z2	3.1e-174	85.96	flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1EZZ4	1.4e-166	80.99	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JE50	2.6e-165	80.99	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1C2J5	4.5e-165	82.70	flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1C6B7	1.7e-159	78.89	flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Sgr021998 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19000.1	1.7e-119	59.25	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.1	2.2e-108	54.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.3	4.7e-98	49.85	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.2	2.4e-86	58.05	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.2	5.4e-78	53.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 216..237 score: 45.45 coord: 281..299 score: 30.14 coord: 46..63 score: 50.51
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 3..335
None	No IPR available	PANTHER	PTHR10209:SF727	DIRIGENT PROTEIN	coord: 3..335
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 6..327
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 26..145 e-value: 4.8E-27 score: 95.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..331 e-value: 2.5E-104 score: 351.2
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 194..293 e-value: 7.1E-29 score: 100.3
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 192..293 score: 13.699693

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr021998.1	Sgr021998.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity