Sgr021977 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021977
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionadenylate isopentenyltransferase 5, chloroplastic-like
Locationtig00153870: 311403 .. 312299 (+)
RNA-Seq ExpressionSgr021977
SyntenySgr021977
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGACAACGTTGAGTACCTGAGCAACAAGAAGCACAAAATCGTGTTCGTAATGGGCTGCACCGCCACCGGAAAATCCAAACTCTCCGTCGACTTGGCCACCTTCTTCCCCTCCGAGATCATCAACTCCGACAAGATTCAGCTCTACCGAGGGCTCGACATCATCACCAACAAGATCACTGAATCCGATCGCCGCGGCGTGCCTCATCATCTTCTCGGAATCATCGAGGATCCCGACGCTGATTTGACTGCCGGAGAGTTTTGCCGCCACGTCGAAGAAGCGATCGCGGAGATTATCGGACGCGGACGGCTTCCGATTGTCGTCGGCGGATCGAACAACTACATTGAAGCACTGGTTGAGAATCCGATTACGGATTTTCGTTCGAGATACGACTGCTGCTTCATTTGGATAGACGTTTCGCTGCCGGTTCTGTACAGGTACATTGCCAAACGAGTCGATCAGATGGTGGACGCAGGGCTGGTGGAGGAGGTCCGGGAGATGTTCGTTCCCGGGGCCGACTACGGCCGCGGAATACGCCGGGCGATCGGCGCTCCTGAGATGGACAGTTACTTCCGAGCCGAGCGAAACGACGAAGCGTACAAGGAAGATTTGCTGAAGACCGCCATTCAAGACATCAAGGACAATACCTGCAAGCTCGCACTCCGTCAGTTCGGGAAAATCCACCGGCTCCGGGACGAGCTAGGTTGGGGACTGCACCGGATCGACGCGACGGCGGTGTTCGAAAAGAGCGGAGAAGACGCCGCCGATGAGTGGATGAATGCGGTTCTGAAACCGAGCTTGGCCATAGTCGGCGATTTCCTCAACGAAAAACATGACGAGAAAACTTCCAAAACAACCAAGAACCGATTCGCGATTACCAATTCGAAGAACTGA

mRNA sequence

ATGAAGGACAACGTTGAGTACCTGAGCAACAAGAAGCACAAAATCGTGTTCGTAATGGGCTGCACCGCCACCGGAAAATCCAAACTCTCCGTCGACTTGGCCACCTTCTTCCCCTCCGAGATCATCAACTCCGACAAGATTCAGCTCTACCGAGGGCTCGACATCATCACCAACAAGATCACTGAATCCGATCGCCGCGGCGTGCCTCATCATCTTCTCGGAATCATCGAGGATCCCGACGCTGATTTGACTGCCGGAGAGTTTTGCCGCCACGTCGAAGAAGCGATCGCGGAGATTATCGGACGCGGACGGCTTCCGATTGTCGTCGGCGGATCGAACAACTACATTGAAGCACTGGTTGAGAATCCGATTACGGATTTTCGTTCGAGATACGACTGCTGCTTCATTTGGATAGACGTTTCGCTGCCGGTTCTGTACAGGTACATTGCCAAACGAGTCGATCAGATGGTGGACGCAGGGCTGGTGGAGGAGGTCCGGGAGATGTTCGTTCCCGGGGCCGACTACGGCCGCGGAATACGCCGGGCGATCGGCGCTCCTGAGATGGACAGTTACTTCCGAGCCGAGCGAAACGACGAAGCGTACAAGGAAGATTTGCTGAAGACCGCCATTCAAGACATCAAGGACAATACCTGCAAGCTCGCACTCCGTCAGTTCGGGAAAATCCACCGGCTCCGGGACGAGCTAGGTTGGGGACTGCACCGGATCGACGCGACGGCGGTGTTCGAAAAGAGCGGAGAAGACGCCGCCGATGAGTGGATGAATGCGGTTCTGAAACCGAGCTTGGCCATAGTCGGCGATTTCCTCAACGAAAAACATGACGAGAAAACTTCCAAAACAACCAAGAACCGATTCGCGATTACCAATTCGAAGAACTGA

Coding sequence (CDS)

ATGAAGGACAACGTTGAGTACCTGAGCAACAAGAAGCACAAAATCGTGTTCGTAATGGGCTGCACCGCCACCGGAAAATCCAAACTCTCCGTCGACTTGGCCACCTTCTTCCCCTCCGAGATCATCAACTCCGACAAGATTCAGCTCTACCGAGGGCTCGACATCATCACCAACAAGATCACTGAATCCGATCGCCGCGGCGTGCCTCATCATCTTCTCGGAATCATCGAGGATCCCGACGCTGATTTGACTGCCGGAGAGTTTTGCCGCCACGTCGAAGAAGCGATCGCGGAGATTATCGGACGCGGACGGCTTCCGATTGTCGTCGGCGGATCGAACAACTACATTGAAGCACTGGTTGAGAATCCGATTACGGATTTTCGTTCGAGATACGACTGCTGCTTCATTTGGATAGACGTTTCGCTGCCGGTTCTGTACAGGTACATTGCCAAACGAGTCGATCAGATGGTGGACGCAGGGCTGGTGGAGGAGGTCCGGGAGATGTTCGTTCCCGGGGCCGACTACGGCCGCGGAATACGCCGGGCGATCGGCGCTCCTGAGATGGACAGTTACTTCCGAGCCGAGCGAAACGACGAAGCGTACAAGGAAGATTTGCTGAAGACCGCCATTCAAGACATCAAGGACAATACCTGCAAGCTCGCACTCCGTCAGTTCGGGAAAATCCACCGGCTCCGGGACGAGCTAGGTTGGGGACTGCACCGGATCGACGCGACGGCGGTGTTCGAAAAGAGCGGAGAAGACGCCGCCGATGAGTGGATGAATGCGGTTCTGAAACCGAGCTTGGCCATAGTCGGCGATTTCCTCAACGAAAAACATGACGAGAAAACTTCCAAAACAACCAAGAACCGATTCGCGATTACCAATTCGAAGAACTGA

Protein sequence

MKDNVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGIRRAIGAPEMDSYFRAERNDEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHDEKTSKTTKNRFAITNSKN
Homology
BLAST of Sgr021977 vs. NCBI nr
Match: XP_038888774.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 513.1 bits (1320), Expect = 1.6e-141
Identity = 249/298 (83.56%), Postives = 274/298 (91.95%), Query Frame = 0

Query: 1   MKDNVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKI 60
           MKD+VEYLSNKKHKIVFVMG TATGKSKLSVDLATFFPSEIINSDKIQ Y+GLDIITNKI
Sbjct: 1   MKDSVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKI 60

Query: 61  TESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALV 120
           TESDRRGVPHHLLG+I+DPDADLTAGEFCR VE++IA+II  GRLPI+VGGSNNYIEALV
Sbjct: 61  TESDRRGVPHHLLGVIDDPDADLTAGEFCRLVEDSIADIISHGRLPIIVGGSNNYIEALV 120

Query: 121 ENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGIR 180
           ENPITDFRSR+DCCF+WIDV+LPVLY+YI KRVDQMV+ GLVEEVR+MFVPGADY RGIR
Sbjct: 121 ENPITDFRSRFDCCFLWIDVALPVLYKYIGKRVDQMVELGLVEEVRDMFVPGADYSRGIR 180

Query: 181 RAIGAPEMDSYFRAERN--DEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWG 240
           RAIGAPE+DSYF+AE+N  +E+YK DLLK+ I +IK+NTCKLALRQFGKIHRLRDELGWG
Sbjct: 181 RAIGAPELDSYFKAEKNNEEESYKNDLLKSGIHEIKENTCKLALRQFGKIHRLRDELGWG 240

Query: 241 LHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKH-DEKTSKTTKNRFAITN 296
           LHR+DATAVFEKSGE+AADEWMNAVLKPSL IVGDFLNEK  D +     KNR AI N
Sbjct: 241 LHRVDATAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQKDYQIEAVIKNRLAIMN 298

BLAST of Sgr021977 vs. NCBI nr
Match: XP_022135701.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Momordica charantia])

HSP 1 Score: 497.3 bits (1279), Expect = 9.3e-137
Identity = 241/288 (83.68%), Postives = 264/288 (91.67%), Query Frame = 0

Query: 3   DNVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITE 62
           D+VEY+S  KHKIVFVMGCTATGKSKL+VDLATF  SEIINSDKIQ YRGLDI+TNKI E
Sbjct: 115 DSVEYVSGYKHKIVFVMGCTATGKSKLAVDLATFLSSEIINSDKIQFYRGLDIVTNKIAE 174

Query: 63  SDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVEN 122
           SDR GVPHHLLGII+DPDADLTAGEFCR +EE IAEIIGR RLPIVVGGSNNYIEALV+N
Sbjct: 175 SDRLGVPHHLLGIIDDPDADLTAGEFCRRLEETIAEIIGRRRLPIVVGGSNNYIEALVDN 234

Query: 123 PITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGIRRA 182
           PIT+FRSR+DCCF+WI+VSLPVLYRYIAKRVD+MV+ GLVEEVREMFVPGA+Y  GIRRA
Sbjct: 235 PITNFRSRFDCCFVWINVSLPVLYRYIAKRVDRMVEVGLVEEVREMFVPGANYSLGIRRA 294

Query: 183 IGAPEMDSYFRAERNDEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLHRI 242
           IG PEMD+YFRAE+N+EAYKEDLLK+AIQ+IKDNTCKLALRQ  KIHRLRDE GWGLHRI
Sbjct: 295 IGVPEMDAYFRAEQNEEAYKEDLLKSAIQEIKDNTCKLALRQLRKIHRLRDERGWGLHRI 354

Query: 243 DATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHDEK-TSKTTKN 290
           DAT VFEKSGEDA DEWMN VLKPSLAIVGDFLNE H++K +SK  +N
Sbjct: 355 DATTVFEKSGEDAVDEWMNVVLKPSLAIVGDFLNEDHEQKISSKAIEN 402

BLAST of Sgr021977 vs. NCBI nr
Match: XP_004148309.1 (adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus])

HSP 1 Score: 496.1 bits (1276), Expect = 2.1e-136
Identity = 244/294 (82.99%), Postives = 267/294 (90.82%), Query Frame = 0

Query: 1   MKDN-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNK 60
           MKDN VEYLSNKKHKIVFVMG TATGKSKLSVDLATFFPSEIINSDKIQ Y+GLDIITNK
Sbjct: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEAL 120
           I +SDRRGVPHHLLG+I DPDADLTAGEFC  VE+AIA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGI 180
           VENPITDFRSR+DCCF+W DV+LPVLY+YIAKRVDQMV+ GLVEEVREMFVPGADY RGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFRAERN--DEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGW 240
           RRAIGAPE+DSYF+AE+N  +E YK DLLK+ I +IK+NTC+LALRQFGKIHRLRDE+GW
Sbjct: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHDEKTSKT-TKNR 291
           GLHRIDATAVFEKSGE+AADEWMN VLKPSL IVG+FLNEK      +T TKNR
Sbjct: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNR 294

BLAST of Sgr021977 vs. NCBI nr
Match: KAE8646129.1 (hypothetical protein Csa_016307 [Cucumis sativus])

HSP 1 Score: 496.1 bits (1276), Expect = 2.1e-136
Identity = 244/294 (82.99%), Postives = 267/294 (90.82%), Query Frame = 0

Query: 1   MKDN-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNK 60
           MKDN VEYLSNKKHKIVFVMG TATGKSKLSVDLATFFPSEIINSDKIQ Y+GLDIITNK
Sbjct: 7   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 66

Query: 61  ITESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEAL 120
           I +SDRRGVPHHLLG+I DPDADLTAGEFC  VE+AIA++I RG LPIVVGGSNNYIEAL
Sbjct: 67  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 126

Query: 121 VENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGI 180
           VENPITDFRSR+DCCF+W DV+LPVLY+YIAKRVDQMV+ GLVEEVREMFVPGADY RGI
Sbjct: 127 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 186

Query: 181 RRAIGAPEMDSYFRAERN--DEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGW 240
           RRAIGAPE+DSYF+AE+N  +E YK DLLK+ I +IK+NTC+LALRQFGKIHRLRDE+GW
Sbjct: 187 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 246

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHDEKTSKT-TKNR 291
           GLHRIDATAVFEKSGE+AADEWMN VLKPSL IVG+FLNEK      +T TKNR
Sbjct: 247 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNR 300

BLAST of Sgr021977 vs. NCBI nr
Match: KAA0036515.1 (adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK13465.1 adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 494.6 bits (1272), Expect = 6.1e-136
Identity = 242/300 (80.67%), Postives = 271/300 (90.33%), Query Frame = 0

Query: 1   MKDN-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNK 60
           MKD+ VEY SNKKHKIVFV+G TATGKSKLSVDLATFFPSEIINSDKIQ Y+GLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEAL 120
           I +SDRRGVPHHLLG+I +PDADLTAGEFCR VE++IA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGI 180
           VENPITDFRSR+DCCF+W DV+LPVLY+YI KRVDQMV+ GLVEEVREMFVPGADY RGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFRAERN--DEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGW 240
           RRAIGAPE++SYF+AE+N  +E YK++LLK+AI +IK+NTCKLALRQFGKIHRLRDELGW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHDEKTSKT-TKNRFAITNS 297
           GLHRID TAVFEKSGE+AADEWMNAVLKPSL IVGDFLNEK      +T TKNR  + N+
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNRMGVMNN 300

BLAST of Sgr021977 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 305.1 bits (780), Expect = 8.9e-82
Identity = 152/273 (55.68%), Postives = 197/273 (72.16%), Query Frame = 0

Query: 11  KKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITESDRRGVPH 70
           +K K+VFVMG T TGKS+L++DLAT FP+EI+NSDKIQ+Y+GLDI+TNK+T  +  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 71  HLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSR 130
           HLLG + D   D TA +F R    A+  I+ R R+PI+ GGSN+YIEALV N   DFR R
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV-NDCVDFRLR 150

Query: 131 YDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVP-GADYGRGIRRAIGAPEMD 190
           Y+CCF+W+DVS PVL+ ++++RVD+MVD GLV+EVR +F P  +DY  GIRRAIG PE+D
Sbjct: 151 YNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELD 210

Query: 191 SYFRAE-RNDEA-YKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLHRIDATAV 250
            + R+E RN  A   E LL+TAI+ IK+NTC LA RQ  KI RL  +  W +HR+DAT V
Sbjct: 211 EFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEV 270

Query: 251 FEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHD 281
           F + GE+A + W N+V  PS   V  FL+   D
Sbjct: 271 FLRRGEEADEAWDNSVAHPSALAVEKFLSYSDD 302

BLAST of Sgr021977 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 287.3 bits (734), Expect = 1.9e-76
Identity = 146/283 (51.59%), Postives = 192/283 (67.84%), Query Frame = 0

Query: 2   KDNVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKIT 61
           K NV    + K K++FVMG T +GKS+L++DLAT F  EIINSDKIQLY+GLD++TNK+T
Sbjct: 23  KVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVT 82

Query: 62  ESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVE 121
             + RGVPHHLLG+ +    +LTA ++ R   +AI+++    +LPIV GGSN+YIEALV 
Sbjct: 83  PKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVN 142

Query: 122 NPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGIRR 181
           +      + YDCCFIW+DVSLPVL  +++KRVD+M++AGL+EEVRE+F P A+Y  GIRR
Sbjct: 143 HSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRR 202

Query: 182 AIGAPEMDSYFRAER-NDEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLH 241
           AIG PE+  Y R E   D A K  +L  A+++IK NT  LA RQ  KI RL  +    +H
Sbjct: 203 AIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWKMSMH 262

Query: 242 RIDATAVFEKSGEDAADE-WMNAVLKPSLAIVGDFLNEKHDEK 283
           R+DAT VF K   +  DE W N V +PS  IV  F N  +  K
Sbjct: 263 RVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNNNQLK 305

BLAST of Sgr021977 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 9.5e-76
Identity = 145/269 (53.90%), Postives = 193/269 (71.75%), Query Frame = 0

Query: 12  KHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITESDRRGVPHH 71
           K K+V +MG T TGKS+LSVD+AT F +EIINSDKIQ+++GLDI+TNKIT  +  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 72  LLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSRY 131
           LLG++  P+ADLTA  +C     +I  ++ RG+LPI+VGGSN+Y+EALV++    FRSRY
Sbjct: 100 LLGVL-PPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRY 159

Query: 132 DCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMF-VPGADYGRGIRRAIGAPEMDS 191
           DCCF+W+DV+LPVL+ ++++RVD+MV++G+VEEVRE F    +DY RGI++AIG PE D 
Sbjct: 160 DCCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDR 219

Query: 192 YFRAER--NDEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLHRIDATAVF 251
           +FR E+  N E  +E+LL   +++IK NT +LA RQ  KI RLR    W + R+DAT VF
Sbjct: 220 FFRNEQFLNVED-REELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVF 279

Query: 252 EK--SGEDAADEWMNAVLKPSLAIVGDFL 276
            K  S  DA   W   V  PS   V  FL
Sbjct: 280 TKRRSKMDANVAWERLVAGPSTDTVSRFL 306

BLAST of Sgr021977 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 4.9e-64
Identity = 133/304 (43.75%), Postives = 185/304 (60.86%), Query Frame = 0

Query: 10  NKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITESDRRGVP 69
           ++K K++ +MG T TGKS+LS+DLA  FP E+INSDK+Q+Y+GLDI TNKI+  DR GVP
Sbjct: 27  HRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVP 86

Query: 70  HHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALV--------- 129
           HHLLG ++    +LT  +F     +A++EI GR +LP++VGGSN++I AL+         
Sbjct: 87  HHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGP 146

Query: 130 ------ENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVP-GA 189
                  + +     RYDCCF+W+DVS+ VL  Y+AKRVD M++ G+ +E+ E + P   
Sbjct: 147 GVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDE 206

Query: 190 DYGR------GIRRAIGAPEMDSYFRAERNDEAYKED---------LLKTAIQDIKDNTC 249
           D+        G+R+AIG PE D YF   R  +   ED           + A++ IK+NTC
Sbjct: 207 DHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTC 266

Query: 250 KLALRQFGKIHRLRDELGWGLHRIDAT-----AVFEKSGEDAADEWMNAVLKPSLAIVGD 278
            LA RQ GKI RL+   GW L R+DAT     A+   SGE   + W   VL+PS+ IV  
Sbjct: 267 HLAKRQIGKILRLKG-AGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSR 326

BLAST of Sgr021977 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 1.9e-63
Identity = 133/290 (45.86%), Postives = 178/290 (61.38%), Query Frame = 0

Query: 10  NKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITESDRRGVP 69
           N+K K+V ++G T  GKS+LSVDLAT FPSEIINSDKIQ+Y GL+I TN+IT  DRRGVP
Sbjct: 62  NRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVP 121

Query: 70  HHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALV----ENPIT 129
           HHLLG+I     +LTAGEF       + EI  R ++PI+ GGSN+++ AL+    +    
Sbjct: 122 HHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 181

Query: 130 DFRS---------RYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVP---GA 189
            F S         RY+CCFIW+DVS  VLY Y+ +RVD+M+D+G+ EE+   + P   G 
Sbjct: 182 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 241

Query: 190 DYGRGIRRAIGAPEMDSYFRAERND------EAYKEDLLKTAIQDIKDNTCKLALRQFGK 249
           +   GIR+AIG PE D YF+    +      +A ++     A+ DIK NT  LA RQ  K
Sbjct: 242 ETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKK 301

Query: 250 IHRLRDELGWGLHRIDATAVFE------KSGEDAADEWMNAVLKPSLAIV 272
           I  L+D  GW + R+DATA F+       S +   + W   VL+PS+ IV
Sbjct: 302 IEMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIV 350

BLAST of Sgr021977 vs. ExPASy TrEMBL
Match: A0A6J1C1H2 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111007596 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.5e-137
Identity = 241/288 (83.68%), Postives = 264/288 (91.67%), Query Frame = 0

Query: 3   DNVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITE 62
           D+VEY+S  KHKIVFVMGCTATGKSKL+VDLATF  SEIINSDKIQ YRGLDI+TNKI E
Sbjct: 115 DSVEYVSGYKHKIVFVMGCTATGKSKLAVDLATFLSSEIINSDKIQFYRGLDIVTNKIAE 174

Query: 63  SDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVEN 122
           SDR GVPHHLLGII+DPDADLTAGEFCR +EE IAEIIGR RLPIVVGGSNNYIEALV+N
Sbjct: 175 SDRLGVPHHLLGIIDDPDADLTAGEFCRRLEETIAEIIGRRRLPIVVGGSNNYIEALVDN 234

Query: 123 PITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGIRRA 182
           PIT+FRSR+DCCF+WI+VSLPVLYRYIAKRVD+MV+ GLVEEVREMFVPGA+Y  GIRRA
Sbjct: 235 PITNFRSRFDCCFVWINVSLPVLYRYIAKRVDRMVEVGLVEEVREMFVPGANYSLGIRRA 294

Query: 183 IGAPEMDSYFRAERNDEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLHRI 242
           IG PEMD+YFRAE+N+EAYKEDLLK+AIQ+IKDNTCKLALRQ  KIHRLRDE GWGLHRI
Sbjct: 295 IGVPEMDAYFRAEQNEEAYKEDLLKSAIQEIKDNTCKLALRQLRKIHRLRDERGWGLHRI 354

Query: 243 DATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHDEK-TSKTTKN 290
           DAT VFEKSGEDA DEWMN VLKPSLAIVGDFLNE H++K +SK  +N
Sbjct: 355 DATTVFEKSGEDAVDEWMNVVLKPSLAIVGDFLNEDHEQKISSKAIEN 402

BLAST of Sgr021977 vs. ExPASy TrEMBL
Match: A0A0A0K7L1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G253720 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.0e-136
Identity = 244/294 (82.99%), Postives = 267/294 (90.82%), Query Frame = 0

Query: 1   MKDN-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNK 60
           MKDN VEYLSNKKHKIVFVMG TATGKSKLSVDLATFFPSEIINSDKIQ Y+GLDIITNK
Sbjct: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEAL 120
           I +SDRRGVPHHLLG+I DPDADLTAGEFC  VE+AIA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGI 180
           VENPITDFRSR+DCCF+W DV+LPVLY+YIAKRVDQMV+ GLVEEVREMFVPGADY RGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFRAERN--DEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGW 240
           RRAIGAPE+DSYF+AE+N  +E YK DLLK+ I +IK+NTC+LALRQFGKIHRLRDE+GW
Sbjct: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHDEKTSKT-TKNR 291
           GLHRIDATAVFEKSGE+AADEWMN VLKPSL IVG+FLNEK      +T TKNR
Sbjct: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNR 294

BLAST of Sgr021977 vs. ExPASy TrEMBL
Match: A0A5D3CPK4 (Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold179G00270 PE=4 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 2.9e-136
Identity = 242/300 (80.67%), Postives = 271/300 (90.33%), Query Frame = 0

Query: 1   MKDN-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNK 60
           MKD+ VEY SNKKHKIVFV+G TATGKSKLSVDLATFFPSEIINSDKIQ Y+GLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEAL 120
           I +SDRRGVPHHLLG+I +PDADLTAGEFCR VE++IA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGI 180
           VENPITDFRSR+DCCF+W DV+LPVLY+YI KRVDQMV+ GLVEEVREMFVPGADY RGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFRAERN--DEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGW 240
           RRAIGAPE++SYF+AE+N  +E YK++LLK+AI +IK+NTCKLALRQFGKIHRLRDELGW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHDEKTSKT-TKNRFAITNS 297
           GLHRID TAVFEKSGE+AADEWMNAVLKPSL IVGDFLNEK      +T TKNR  + N+
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNRMGVMNN 300

BLAST of Sgr021977 vs. ExPASy TrEMBL
Match: A0A1S3BH37 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489774 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 1.9e-135
Identity = 241/300 (80.33%), Postives = 270/300 (90.00%), Query Frame = 0

Query: 1   MKDN-VEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNK 60
           MKD+ VEY SNKKHKIVFV+G TATGKSKLSVDLATFFPSEIINSDKIQ Y+GLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  ITESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEAL 120
           I +SDRRGVPHHLLG+I +PDADLTAGEFCR VE++IA++I RG LPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGI 180
           VENPITDFRSR+DCCF+W DV+LPVLY+YI KRVDQMV+ GLVEEV EMFVPGADY RGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVGEMFVPGADYSRGI 180

Query: 181 RRAIGAPEMDSYFRAERN--DEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGW 240
           RRAIGAPE++SYF+AE+N  +E YK++LLK+AI +IK+NTCKLALRQFGKIHRLRDELGW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHDEKTSKT-TKNRFAITNS 297
           GLHRID TAVFEKSGE+AADEWMNAVLKPSL IVGDFLNEK      +T TKNR  + N+
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNRMGVMNN 300

BLAST of Sgr021977 vs. ExPASy TrEMBL
Match: A0A6J1GLR3 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111455496 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 3.0e-133
Identity = 235/297 (79.12%), Postives = 262/297 (88.22%), Query Frame = 0

Query: 1   MKDNVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKI 60
           M  +VEYLSNKKHK++FVMGCTATGKSKLSVDLA FFPSEIINSDKIQ Y GLDIITNKI
Sbjct: 4   MNHSVEYLSNKKHKVIFVMGCTATGKSKLSVDLANFFPSEIINSDKIQFYEGLDIITNKI 63

Query: 61  TESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALV 120
            ESDRRGVPHHLLG+I+DPDADLTA EFCR VE++IAEIIGRGR+PI+VGGSNNYIEALV
Sbjct: 64  VESDRRGVPHHLLGVIQDPDADLTAAEFCRLVEDSIAEIIGRGRIPIIVGGSNNYIEALV 123

Query: 121 ENPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGIR 180
           ENPI DFRSR+DCCF+W DV+LPVL RYI+KRVDQMV+AGLVEEVREM+VP ADY RGIR
Sbjct: 124 ENPIADFRSRFDCCFVWTDVALPVLNRYISKRVDQMVEAGLVEEVREMYVPNADYSRGIR 183

Query: 181 RAIGAPEMDSYFRAERNDEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLH 240
           RAIGAPEMDSYF++E+ND++    LLK+AIQ+IKDNTC LALRQ GKIHRLRDELGWG+H
Sbjct: 184 RAIGAPEMDSYFKSEKNDDS----LLKSAIQEIKDNTCTLALRQRGKIHRLRDELGWGIH 243

Query: 241 RIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFLNEKH-DEKTSKTTKNRFAITNS 297
           RIDATAVFE SGED AD WMN VLKPSL IVG FLN++H D +    TKN F   +S
Sbjct: 244 RIDATAVFENSGEDTADVWMNTVLKPSLEIVGKFLNKEHKDYRIETMTKNGFTTNSS 296

BLAST of Sgr021977 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 305.1 bits (780), Expect = 6.3e-83
Identity = 152/273 (55.68%), Postives = 197/273 (72.16%), Query Frame = 0

Query: 11  KKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITESDRRGVPH 70
           +K K+VFVMG T TGKS+L++DLAT FP+EI+NSDKIQ+Y+GLDI+TNK+T  +  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 71  HLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSR 130
           HLLG + D   D TA +F R    A+  I+ R R+PI+ GGSN+YIEALV N   DFR R
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV-NDCVDFRLR 150

Query: 131 YDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVP-GADYGRGIRRAIGAPEMD 190
           Y+CCF+W+DVS PVL+ ++++RVD+MVD GLV+EVR +F P  +DY  GIRRAIG PE+D
Sbjct: 151 YNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELD 210

Query: 191 SYFRAE-RNDEA-YKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLHRIDATAV 250
            + R+E RN  A   E LL+TAI+ IK+NTC LA RQ  KI RL  +  W +HR+DAT V
Sbjct: 211 EFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEV 270

Query: 251 FEKSGEDAADEWMNAVLKPSLAIVGDFLNEKHD 281
           F + GE+A + W N+V  PS   V  FL+   D
Sbjct: 271 FLRRGEEADEAWDNSVAHPSALAVEKFLSYSDD 302

BLAST of Sgr021977 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 287.3 bits (734), Expect = 1.4e-77
Identity = 146/283 (51.59%), Postives = 192/283 (67.84%), Query Frame = 0

Query: 2   KDNVEYLSNKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKIT 61
           K NV    + K K++FVMG T +GKS+L++DLAT F  EIINSDKIQLY+GLD++TNK+T
Sbjct: 23  KVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVT 82

Query: 62  ESDRRGVPHHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVE 121
             + RGVPHHLLG+ +    +LTA ++ R   +AI+++    +LPIV GGSN+YIEALV 
Sbjct: 83  PKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVN 142

Query: 122 NPITDFRSRYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVPGADYGRGIRR 181
           +      + YDCCFIW+DVSLPVL  +++KRVD+M++AGL+EEVRE+F P A+Y  GIRR
Sbjct: 143 HSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRR 202

Query: 182 AIGAPEMDSYFRAER-NDEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLH 241
           AIG PE+  Y R E   D A K  +L  A+++IK NT  LA RQ  KI RL  +    +H
Sbjct: 203 AIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWKMSMH 262

Query: 242 RIDATAVFEKSGEDAADE-WMNAVLKPSLAIVGDFLNEKHDEK 283
           R+DAT VF K   +  DE W N V +PS  IV  F N  +  K
Sbjct: 263 RVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNNNQLK 305

BLAST of Sgr021977 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 285.0 bits (728), Expect = 6.8e-77
Identity = 145/269 (53.90%), Postives = 193/269 (71.75%), Query Frame = 0

Query: 12  KHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITESDRRGVPHH 71
           K K+V +MG T TGKS+LSVD+AT F +EIINSDKIQ+++GLDI+TNKIT  +  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 72  LLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVENPITDFRSRY 131
           LLG++  P+ADLTA  +C     +I  ++ RG+LPI+VGGSN+Y+EALV++    FRSRY
Sbjct: 100 LLGVL-PPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRY 159

Query: 132 DCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMF-VPGADYGRGIRRAIGAPEMDS 191
           DCCF+W+DV+LPVL+ ++++RVD+MV++G+VEEVRE F    +DY RGI++AIG PE D 
Sbjct: 160 DCCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDR 219

Query: 192 YFRAER--NDEAYKEDLLKTAIQDIKDNTCKLALRQFGKIHRLRDELGWGLHRIDATAVF 251
           +FR E+  N E  +E+LL   +++IK NT +LA RQ  KI RLR    W + R+DAT VF
Sbjct: 220 FFRNEQFLNVED-REELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVF 279

Query: 252 EK--SGEDAADEWMNAVLKPSLAIVGDFL 276
            K  S  DA   W   V  PS   V  FL
Sbjct: 280 TKRRSKMDANVAWERLVAGPSTDTVSRFL 306

BLAST of Sgr021977 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 244.2 bits (622), Expect = 1.3e-64
Identity = 133/290 (45.86%), Postives = 178/290 (61.38%), Query Frame = 0

Query: 10  NKKHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITESDRRGVP 69
           N+K K+V ++G T  GKS+LSVDLAT FPSEIINSDKIQ+Y GL+I TN+IT  DRRGVP
Sbjct: 62  NRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVP 121

Query: 70  HHLLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALV----ENPIT 129
           HHLLG+I     +LTAGEF       + EI  R ++PI+ GGSN+++ AL+    +    
Sbjct: 122 HHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 181

Query: 130 DFRS---------RYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVP---GA 189
            F S         RY+CCFIW+DVS  VLY Y+ +RVD+M+D+G+ EE+   + P   G 
Sbjct: 182 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 241

Query: 190 DYGRGIRRAIGAPEMDSYFRAERND------EAYKEDLLKTAIQDIKDNTCKLALRQFGK 249
           +   GIR+AIG PE D YF+    +      +A ++     A+ DIK NT  LA RQ  K
Sbjct: 242 ETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKK 301

Query: 250 IHRLRDELGWGLHRIDATAVFE------KSGEDAADEWMNAVLKPSLAIV 272
           I  L+D  GW + R+DATA F+       S +   + W   VL+PS+ IV
Sbjct: 302 IEMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIV 350

BLAST of Sgr021977 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 221.5 bits (563), Expect = 9.2e-58
Identity = 122/288 (42.36%), Postives = 174/288 (60.42%), Query Frame = 0

Query: 12  KHKIVFVMGCTATGKSKLSVDLATFFPSEIINSDKIQLYRGLDIITNKITESDRRGVPHH 71
           K K+V +MG T +GKS LS+DLAT F  EI+NSDKIQ Y GL + TN+++  +R GVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 72  LLGIIEDPDADLTAGEFCRHVEEAIAEIIGRGRLPIVVGGSNNYIEALVEN-------PI 131
           LLG +   D++LT  EF      +I+EI  RG LPI+ GGSN++I AL+ +       P 
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPF 161

Query: 132 TDFRS-----RYDCCFIWIDVSLPVLYRYIAKRVDQMVDAGLVEEVREMFVP----GADY 191
           +   S     RY+CCF+W+DVS+ VL+ Y++KRVDQM+++G+ EE+   + P     A  
Sbjct: 162 SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 221

Query: 192 GRGIRRAIGAPEMDSYFR---AERNDEAYKEDLLK-----TAIQDIKDNTCKLALRQFGK 251
             GI + IG PE D YF     ER  +  + D  +      A+Q+IK+NT +LA +Q  +
Sbjct: 222 AHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIER 281

Query: 252 IHRLRDELGWGLHRIDATAVFEKSGEDAADEWMNAVLKPSLAIVGDFL 276
           I +L+   GW + R+DAT  F   G  + + W N VL  S+ +V  FL
Sbjct: 282 IMKLKSS-GWDIQRLDATPSF---GRSSREIWDNTVLDESIKVVKRFL 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888774.11.6e-14183.56adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida][more]
XP_022135701.19.3e-13783.68adenylate isopentenyltransferase 5, chloroplastic-like [Momordica charantia][more]
XP_004148309.12.1e-13682.99adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus][more]
KAE8646129.12.1e-13682.99hypothetical protein Csa_016307 [Cucumis sativus][more]
KAA0036515.16.1e-13680.67adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK13465.1 adenyl... [more]
Match NameE-valueIdentityDescription
Q94ID28.9e-8255.68Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID11.9e-7651.59Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q93WC99.5e-7653.90Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q5GHF74.9e-6443.75Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID31.9e-6345.86Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1C1H24.5e-13783.68adenylate isopentenyltransferase 5, chloroplastic-like OS=Momordica charantia OX... [more]
A0A0A0K7L11.0e-13682.99Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G253720 PE=4 SV=1[more]
A0A5D3CPK42.9e-13680.67Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BH371.9e-13580.33adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 G... [more]
A0A6J1GLR33.0e-13379.12adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
AT5G19040.16.3e-8355.68isopentenyltransferase 5 [more]
AT3G23630.11.4e-7751.59isopentenyltransferase 7 [more]
AT3G63110.16.8e-7753.90isopentenyltransferase 3 [more]
AT1G68460.11.3e-6445.86isopentenyltransferase 1 [more]
AT3G19160.19.2e-5842.36ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 12..275
e-value: 1.2E-107
score: 361.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 10..171
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 142..239
e-value: 1.2E-107
score: 361.0
NoneNo IPR availablePFAMPF01715IPPTcoord: 129..225
e-value: 1.5E-13
score: 51.0
coord: 48..125
e-value: 1.4E-22
score: 80.6
NoneNo IPR availablePANTHERPTHR11088:SF67BINDING PROTEIN, PUTATIVE-RELATEDcoord: 7..278
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 7..278

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021977.1Sgr021977.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0052381 tRNA dimethylallyltransferase activity