Sgr021813 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr021813
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	AB hydrolase-1 domain-containing protein
Location	tig00153836: 535141 .. 537314 (-)
RNA-Seq Expression	Sgr021813
Synteny	Sgr021813

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGATAAGAGTAACGGGACCACATTCAGGCTTTGTTTGGTTGCAGGTGCATGTGAATTTGCAGCAAATAATTCAGAAAAAGAGGAACTCCTTTGTTAGACACACCCACAGGGCAGGCAGGTTTGTTGGTCTCAGTAAGAATCCAACCGCAAGTGCAGCTGTGATTACTGCAACATTGTCAGCATCTGTGGGCATTGGGGCTGCTCTCGAGGTGCAAGAAATACGGGAGAGATCCAACAAGTGGCAATGGAACCAGTACTCCATAAACTTTTTCGTTTCTCATTATCAACCTTCTCAACAGCAACCACAATCAAGTTCTAATCCTCCGGTTGTCCTTTTAGTTCATGGATTTGGAGCTTCTATTCCTCATTGGCGCAGGTCTATATCTTTCTCTCCACTTTATTAACTTTTAAACATTAAAATTTATAGGTTTCTCACATATTTAACTTAATTTTGAAGGATAAAAATTCGCTTCTTCTTGTTCTTGCTTTAAGTAGTATCACCCGATTTAACCCCTAAATTTAATGCATTTTCCATTTCATTATGGTTCAAGAAGTCTAATAGGGTGCATTATGAATCATTGATTAACTAATTCTTAGTACCATCAAATTATATTTAGTACTATTTTTAGATTGACAATCTCTTTATGGATTTTTCTTTTCCTTTTTTTCTGTTAATTTATTTCAAAGTTTTTTGACATTCTTGTAATTTCTCTCTCCCTCTCTCACTCTTTTTTTTTTTAAATAATTTCTCGAGGTATCTTTTTTCCTTAGGATTTTATTCTTACTCTCCCCATAGTCCAATAGACTCTGGTGATTTGTTGTGCTATACGAGTACAAGCCTTTGGTCAGTGTAGTGGACTACCATTACACCACTTTATTCACAGAGAATTCCAGCGATCAACTAGGTCATACATCAAGGAGTGCAACATGCCTTTATTCAGAGATTTGTTGTTCTTGTCATGCATGTCACCGAGCCCTGATATATTATTTGGAGATTTCTTCAGGAATATTAGCACATTGTCTCAATGTTCAACTGTCTATGCCATCGACCTTCTTGGCTTCGGTGCTTCCGATAAGCCTGTCGGCTTTGCATATACCATGGAAACATGGGCAAATGTATGTTCACATGGAAGCTTCAATTATGTATTTATTCTTATTCGTTGATGACCAATTATCGATCGGTAGGAATAGGAGCTCACTAACGTCACTAATGTGGCCCGTACGCATTGGTGACAGTTGATATTGGATTTTGTGGATCAAGTTATTAAGAAGCCAACTGTGCTAGTGGGTAACTCAGTGGGAAGCCTTGCTTGTGTGATTGCTGCTTCAGGTGCGTGAAGCCAACATTTACAGAAATAAATCCTTAGAAAGAACTCTTGATTTGTGGAAGTGTTTTGTATGTTTCCCATGAAACCAGATTCTAGGCGCAATCTAGTTCAAGGACTTGTGCTCTTAAACTGTGCTGGTGGTATGAACAATAAAGCAATCGTCGATGATTGGAGGATCAAACTGTTGTTACCTTTGCTTTGGCTGTTCGATTTCTTACTGAAGCAACGGGGAATTGCTTCAGCGCTGTTTGAACGTGTGAAACAGAGGTATTTATATTTGTATTTGTTTTACTTATAATCAACTTTTCCTTTTCCTTGTGGCTAAATGGAGGAGAAAATGAAAGTGAGAAATTGTGATATTTTCCCGCAGAGAGAGCTTGAGGAACATTTTGTTGTCCGTATATGGAAACAAGGAAAGTGTGAATGAAGATCTGATAGACGTAGGGGCCCTTTCTCCCCTCCAAGTTACCTCTCTCTCTTCTTCCTCGGATTAATGATGTTCTAACCTTGTTTTGAACTGATGAATATAGATAATTGTGGAACCAGCAAACGATGTAGGGGCACTTGATGCTTTCGTTTCAATCGTGACAGGGCCACCAGGGCCGAGCCCGGTGCAACTAATGCCCAGAGTTTCTGTGCCAGTTCTGGTTTTATGGGGAGATGAAGATCCATTCACCCCACTTGATGGCCCTGTTGGCAAGTACTTCTCTAATTTATCTATGGAATTACCAAATGTAAGTCTTTTTGTGTTGAAAGGAGTAGGACATTGCCCTCATGATGATAGACCTGAATTAGTGCATGAAAAGCTCCTTCCATGGCTGGCTCAGGTTCCTGCTTTGTGA

mRNA sequence

ATGGGGATAAGAGTAACGGGACCACATTCAGGCTTTGTTTGGTTGCAGGTGCATGTGAATTTGCAGCAAATAATTCAGAAAAAGAGGAACTCCTTTGTTAGACACACCCACAGGGCAGGCAGGTTTGTTGGTCTCAGTAAGAATCCAACCGCAAGTGCAGCTGTGATTACTGCAACATTGTCAGCATCTGTGGGCATTGGGGCTGCTCTCGAGGTGCAAGAAATACGGGAGAGATCCAACAAGTGGCAATGGAACCAGTACTCCATAAACTTTTTCGTTTCTCATTATCAACCTTCTCAACAGCAACCACAATCAAGTTCTAATCCTCCGGTTGTCCTTTTAGTTCATGGATTTGGAGCTTCTATTCCTCATTGGCGCAGGAATATTAGCACATTGTCTCAATGTTCAACTGTCTATGCCATCGACCTTCTTGGCTTCGGTGCTTCCGATAAGCCTGTCGGCTTTGCATATACCATGGAAACATGGGCAAATTTGATATTGGATTTTGTGGATCAAGTTATTAAGAAGCCAACTGTGCTAGTGGGTAACTCAGTGGGAAGCCTTGCTTGTGTGATTGCTGCTTCAGATTCTAGGCGCAATCTAGTTCAAGGACTTGTGCTCTTAAACTGTGCTGGTGGTATGAACAATAAAGCAATCGTCGATGATTGGAGGATCAAACTGTTGTTACCTTTGCTTTGGCTGTTCGATTTCTTACTGAAGCAACGGGGAATTGCTTCAGCGCTGTTTGAACGTGTGAAACAGAGAGAGAGCTTGAGGAACATTTTGTTGTCCGTATATGGAAACAAGGAAAGTGTGAATGAAGATCTGATAGACATAATTGTGGAACCAGCAAACGATGTAGGGGCACTTGATGCTTTCGTTTCAATCGTGACAGGGCCACCAGGGCCGAGCCCGGTGCAACTAATGCCCAGAGTTTCTGTGCCAGTTCTGGTTTTATGGGGAGATGAAGATCCATTCACCCCACTTGATGGCCCTGTTGGCAAGTACTTCTCTAATTTATCTATGGAATTACCAAATGTAAGTCTTTTTGTGTTGAAAGGAGTAGGACATTGCCCTCATGATGATAGACCTGAATTAGTGCATGAAAAGCTCCTTCCATGGCTGGCTCAGGTTCCTGCTTTGTGA

Coding sequence (CDS)

ATGGGGATAAGAGTAACGGGACCACATTCAGGCTTTGTTTGGTTGCAGGTGCATGTGAATTTGCAGCAAATAATTCAGAAAAAGAGGAACTCCTTTGTTAGACACACCCACAGGGCAGGCAGGTTTGTTGGTCTCAGTAAGAATCCAACCGCAAGTGCAGCTGTGATTACTGCAACATTGTCAGCATCTGTGGGCATTGGGGCTGCTCTCGAGGTGCAAGAAATACGGGAGAGATCCAACAAGTGGCAATGGAACCAGTACTCCATAAACTTTTTCGTTTCTCATTATCAACCTTCTCAACAGCAACCACAATCAAGTTCTAATCCTCCGGTTGTCCTTTTAGTTCATGGATTTGGAGCTTCTATTCCTCATTGGCGCAGGAATATTAGCACATTGTCTCAATGTTCAACTGTCTATGCCATCGACCTTCTTGGCTTCGGTGCTTCCGATAAGCCTGTCGGCTTTGCATATACCATGGAAACATGGGCAAATTTGATATTGGATTTTGTGGATCAAGTTATTAAGAAGCCAACTGTGCTAGTGGGTAACTCAGTGGGAAGCCTTGCTTGTGTGATTGCTGCTTCAGATTCTAGGCGCAATCTAGTTCAAGGACTTGTGCTCTTAAACTGTGCTGGTGGTATGAACAATAAAGCAATCGTCGATGATTGGAGGATCAAACTGTTGTTACCTTTGCTTTGGCTGTTCGATTTCTTACTGAAGCAACGGGGAATTGCTTCAGCGCTGTTTGAACGTGTGAAACAGAGAGAGAGCTTGAGGAACATTTTGTTGTCCGTATATGGAAACAAGGAAAGTGTGAATGAAGATCTGATAGACATAATTGTGGAACCAGCAAACGATGTAGGGGCACTTGATGCTTTCGTTTCAATCGTGACAGGGCCACCAGGGCCGAGCCCGGTGCAACTAATGCCCAGAGTTTCTGTGCCAGTTCTGGTTTTATGGGGAGATGAAGATCCATTCACCCCACTTGATGGCCCTGTTGGCAAGTACTTCTCTAATTTATCTATGGAATTACCAAATGTAAGTCTTTTTGTGTTGAAAGGAGTAGGACATTGCCCTCATGATGATAGACCTGAATTAGTGCATGAAAAGCTCCTTCCATGGCTGGCTCAGGTTCCTGCTTTGTGA

Protein sequence

MGIRVTGPHSGFVWLQVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLSASVGIGAALEVQEIRERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQCSTVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQRESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVPVLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQVPAL

Homology

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BLAST of Sgr021813 vs. NCBI nr
Match: XP_022156028.1 (pheophytinase, chloroplastic [Momordica charantia])

HSP 1 Score: 673.3 bits (1736), Expect = 1.2e-189
Identity = 336/368 (91.30%), Postives = 349/368 (94.84%), Query Frame = 0


Query: 16  QVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLSASVGIGAALEVQEI 75
           +V VN QQI+QKKRN FVRH+HRAG FVGLSKN +A AAVITAT SASV IGAALEVQEI
Sbjct: 25  RVDVNFQQIVQKKRNCFVRHSHRAGGFVGLSKNQSAGAAVITATSSASVAIGAALEVQEI 84

Query: 76  RERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQC 135
           RERSNKWQWNQYSIN+FVSHYQPSQQQ QSSSNPP+VLLVHGFGASIPHWRRNISTLSQ 
Sbjct: 85  RERSNKWQWNQYSINYFVSHYQPSQQQSQSSSNPPIVLLVHGFGASIPHWRRNISTLSQY 144

Query: 136 STVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 195
           STVYAIDLLGFGASDKP GFAYTMETWA+LILDFVDQVIKKPTVLVGNSVGSLACVIAAS
Sbjct: 145 STVYAIDLLGFGASDKPAGFAYTMETWAHLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 204

Query: 196 DSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQR 255
           DS RNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLLKQRGIAS LFERVKQR
Sbjct: 205 DSSRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLKQRGIASVLFERVKQR 264

Query: 256 ESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVP 315
           +SLRNILLSVYGNKESV+EDLIDIIVEPAND GALDAFVSIVTGPPGPSPVQLMPRVSVP
Sbjct: 265 DSLRNILLSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPVQLMPRVSVP 324

Query: 316 VLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWL 375
           VL+LWGDEDPFTPLDGPVG+YFS LSME PNVSLFVLKGVGHCPHDDRPELVH+KLLPWL
Sbjct: 325 VLILWGDEDPFTPLDGPVGRYFSKLSMEFPNVSLFVLKGVGHCPHDDRPELVHQKLLPWL 384

Query: 376 AQV--PAL 382
           AQ+  PAL
Sbjct: 385 AQLQRPAL 392

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BLAST of Sgr021813 vs. NCBI nr
Match: XP_038884599.1 (pheophytinase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 659.8 bits (1701), Expect = 1.4e-185
Identity = 326/366 (89.07%), Postives = 347/366 (94.81%), Query Frame = 0


Query: 16  QVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLSASVGIGAALEVQEI 75
           ++HVNLQ+IIQ  RN F+RH + AGRFVGLSKNP+  AAVITAT SASV IG ALEVQEI
Sbjct: 63  RLHVNLQKIIQGNRNCFIRHRNWAGRFVGLSKNPSTKAAVITATSSASVCIGPALEVQEI 122

Query: 76  RERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQC 135
           RERSNKWQWNQYSIN+FVSHYQPS++QPQSSS+PPVVLLVHGFGASIPHWRRNISTLSQ 
Sbjct: 123 RERSNKWQWNQYSINYFVSHYQPSREQPQSSSSPPVVLLVHGFGASIPHWRRNISTLSQY 182

Query: 136 STVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 195
           STVYAIDLLGFGAS+KP GFAYTMETWA+LILDFVDQVIKKPTVLVGNSVGSLACVIAAS
Sbjct: 183 STVYAIDLLGFGASNKPAGFAYTMETWADLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 242

Query: 196 DSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQR 255
           DS RNLV+GLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLL QR IASALFERVK+R
Sbjct: 243 DSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRVIASALFERVKKR 302

Query: 256 ESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVP 315
           +SLRNILLSVYGNKESV+EDLIDIIVEPAND GALDAFVSIVTGPPGPSPVQLMP++SVP
Sbjct: 303 DSLRNILLSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPVQLMPKISVP 362

Query: 316 VLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWL 375
           VL+LWGDEDPFTPLDGPVGKYFS LS E+PNVSLFVLKGVGHCPHDDRPE VHEKLLPWL
Sbjct: 363 VLILWGDEDPFTPLDGPVGKYFSKLSKEVPNVSLFVLKGVGHCPHDDRPESVHEKLLPWL 422

Query: 376 AQVPAL 382
           AQ+PAL
Sbjct: 423 AQLPAL 428

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BLAST of Sgr021813 vs. NCBI nr
Match: XP_023523832.1 (pheophytinase, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-184
Identity = 325/366 (88.80%), Postives = 346/366 (94.54%), Query Frame = 0


Query: 16  QVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLSASVGIGAALEVQEI 75
           QVHVNLQQIIQKKRN FVR+++RAGRF GLSKNP+ASAAVI+AT SASVG+GAALEVQEI
Sbjct: 76  QVHVNLQQIIQKKRNRFVRYSNRAGRFFGLSKNPSASAAVISATSSASVGVGAALEVQEI 135

Query: 76  RERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQC 135
           RERSNKWQWNQYSIN+FVSHYQPSQQQ QSS +PPVVLLVHGFGASIPHWRRNIS LSQ 
Sbjct: 136 RERSNKWQWNQYSINYFVSHYQPSQQQHQSSVDPPVVLLVHGFGASIPHWRRNISALSQY 195

Query: 136 STVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 195
           STVYAIDLLGFGASDKP GFAYTMETWA+LILDFV  +IK+PTVLVGNSVGSLACVIAAS
Sbjct: 196 STVYAIDLLGFGASDKPAGFAYTMETWADLILDFVKDIIKRPTVLVGNSVGSLACVIAAS 255

Query: 196 DSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQR 255
           DS RNLV+GLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLL QR IASALFERVK+R
Sbjct: 256 DSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLNQRRIASALFERVKKR 315

Query: 256 ESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVP 315
           +SLRNIL SVYGNKE+V+EDLIDII+EPAND GALDAFVSIVTGPPGPSPVQLMP++SVP
Sbjct: 316 DSLRNILSSVYGNKENVDEDLIDIILEPANDEGALDAFVSIVTGPPGPSPVQLMPKISVP 375

Query: 316 VLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWL 375
           VL+LWGDEDPFTPLDGPVGKYFS LSME PNVSL VLKGVGHCPHDDRPELVHEKLLPWL
Sbjct: 376 VLILWGDEDPFTPLDGPVGKYFSKLSMEAPNVSLCVLKGVGHCPHDDRPELVHEKLLPWL 435

Query: 376 AQVPAL 382
           AQ+ AL
Sbjct: 436 AQLHAL 441

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BLAST of Sgr021813 vs. NCBI nr
Match: XP_022932467.1 (pheophytinase, chloroplastic [Cucurbita moschata] >XP_022932468.1 pheophytinase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 654.4 bits (1687), Expect = 5.8e-184
Identity = 323/366 (88.25%), Postives = 346/366 (94.54%), Query Frame = 0


Query: 16  QVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLSASVGIGAALEVQEI 75
           QVHVN+QQIIQKKRN FVR+++RAGRF GLSKNP+ASAAV+TAT SASVG+GAALEVQEI
Sbjct: 76  QVHVNVQQIIQKKRNLFVRYSNRAGRFFGLSKNPSASAAVVTATSSASVGVGAALEVQEI 135

Query: 76  RERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQC 135
           RERSNKWQWNQYSIN+FVSHYQPSQQQ QSS +PPVVLLVHGFGASIPHWRRNIS LSQ 
Sbjct: 136 RERSNKWQWNQYSINYFVSHYQPSQQQHQSSVDPPVVLLVHGFGASIPHWRRNISALSQY 195

Query: 136 STVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 195
           STVYAIDLLGFGASDKP GFAYTMETWA+LILDFV  +IK+PTVLVGNSVGSLACVIAAS
Sbjct: 196 STVYAIDLLGFGASDKPAGFAYTMETWADLILDFVKDIIKRPTVLVGNSVGSLACVIAAS 255

Query: 196 DSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQR 255
           DS RNLV+GLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLL QR IASALFERVK+R
Sbjct: 256 DSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLNQRRIASALFERVKKR 315

Query: 256 ESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVP 315
           +SLRNIL SVYGNKE+V+EDLIDII+EPAND GALDAFVSIVTGPPGPSPVQLMP++SVP
Sbjct: 316 DSLRNILSSVYGNKENVDEDLIDIILEPANDEGALDAFVSIVTGPPGPSPVQLMPKISVP 375

Query: 316 VLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWL 375
           VL+LWGDEDPFTPLDGPVGKYFS LSME PNVSL VL+GVGHCPHDDRPELVHEKLLPWL
Sbjct: 376 VLILWGDEDPFTPLDGPVGKYFSKLSMEAPNVSLCVLEGVGHCPHDDRPELVHEKLLPWL 435

Query: 376 AQVPAL 382
           AQ+ AL
Sbjct: 436 AQLHAL 441

[Close]

BLAST of Sgr021813 vs. NCBI nr
Match: KAA0047761.1 (pheophytinase [Cucumis melo var. makuwa] >TYK02393.1 pheophytinase [Cucumis melo var. makuwa])

HSP 1 Score: 649.8 bits (1675), Expect = 1.4e-182
Identity = 325/380 (85.53%), Postives = 350/380 (92.11%), Query Frame = 0


Query: 3   IRVTGPHSGFVWLQVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLS- 62
           +RV GPHSGF+WLQV VNLQQ   + +N F+R  + AGRF+GLSKNP+  AAVITA  S 
Sbjct: 1   MRVMGPHSGFIWLQVLVNLQQTFLRNQNCFIRCRNWAGRFIGLSKNPSTKAAVITAASSA 60

Query: 63  ASVGIGAALEVQEIRERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGAS 122
           ASV IG ALEVQEIRERSNKWQWNQYSIN+FVSHYQP QQQPQSSS+ PVVLLVHGFGAS
Sbjct: 61  ASVCIGPALEVQEIRERSNKWQWNQYSINYFVSHYQP-QQQPQSSSS-PVVLLVHGFGAS 120

Query: 123 IPHWRRNISTLSQCSTVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLV 182
           IPHWRRNISTLSQ STVYAIDLLGFGASDKP GFAYTMETWA L++DFVDQ+IKKPTVLV
Sbjct: 121 IPHWRRNISTLSQYSTVYAIDLLGFGASDKPAGFAYTMETWAELVIDFVDQIIKKPTVLV 180

Query: 183 GNSVGSLACVIAASDSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQ 242
           GNSVGSLACVIAASDS RNLV+GLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLL Q
Sbjct: 181 GNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLNQ 240

Query: 243 RGIASALFERVKQRESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPP 302
           R IASALF+RVK+R+SLRNIL SVYGNKESV+EDLIDIIVEPAND GALDAFVSIVTGPP
Sbjct: 241 RIIASALFKRVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPP 300

Query: 303 GPSPVQLMPRVSVPVLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHD 362
           GPSPV+LMP++SVPVL+LWGD+DPFTPLDGPVGKYF  LSME+PNVSLFVLKGVGHCPHD
Sbjct: 301 GPSPVELMPKLSVPVLILWGDKDPFTPLDGPVGKYFRKLSMEVPNVSLFVLKGVGHCPHD 360

Query: 363 DRPELVHEKLLPWLAQVPAL 382
           DRPELVHEKLLPWLAQ+PAL
Sbjct: 361 DRPELVHEKLLPWLAQLPAL 378

[Close]

BLAST of Sgr021813 vs. ExPASy Swiss-Prot
Match: Q9FFZ1 (Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 2.5e-20
Identity = 82/305 (26.89%), Postives = 137/305 (44.92%), Query Frame = 0


Query: 110 PVVLLVHGFGASIPHWRRNISTLSQCSTVYAIDLLGFGAS-------------------- 169
           P VL + GFG    H+ + ++ L +   V+AID LG G S                    
Sbjct: 117 PAVLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLSLPTEDPTTMTEETSSSEDKE 176

Query: 170 ------DKPVGFA----YTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSRR 229
                 DK   +A    ++++ W + +  FV++VI +P  + GNS+G    +  A+ +  
Sbjct: 177 PFWGFGDKTEPWADQLVFSLDLWRDQVQYFVEEVIGEPVYIAGNSLGGYVALYFAA-THP 236

Query: 230 NLVQGLVLLNCA---GGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQRE 289
           +LV+G+ LLN     G   N   V   ++  L P    F    + + I   +++++   E
Sbjct: 237 HLVKGVTLLNATPFWGFFPNP--VRSPKLARLFPWPGAFPLPERVKKITELVWQKISDPE 296

Query: 290 SLRNILLSVYGNKESVNED-LIDIIVEPANDVGALDAFVSIVTGPPG----PSPVQLMPR 349
           S+  IL  VY    S+N D +   IVE      A  +F SI+  P G       +     
Sbjct: 297 SIAEILKQVY-TDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPGGELSFSEALSRCKE 356

Query: 350 VSVPVLVLWGDEDPFT-PLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEK 376
            +V + +++G EDP+  PL G        +  E+PN   + +   GHCPHD+ PE+V+  
Sbjct: 357 NNVQICLMYGREDPWVRPLWG------KKIKKEIPNAPYYEISPAGHCPHDEVPEVVNYL 411

[Close]

BLAST of Sgr021813 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 3.3e-20
Identity = 79/290 (27.24%), Postives = 135/290 (46.55%), Query Frame = 0


Query: 89  INFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQCSTVYAIDLLGFGA 148
           +N +  HYQ   +Q         ++ VHGF +S   +R+ I  L     + A+DL  FG 
Sbjct: 15  VNVYYEHYQNPGRQ--------TLVCVHGFLSSAFSFRKVIPLLRDKYDIIALDLPPFGQ 74

Query: 149 SDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSRRNLVQGLVLL 208
           S+K   F YT +  A L++  ++ +  K  VLVG+S+G    + AA   +  L   +VLL
Sbjct: 75  SEKSRTFIYTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAAL-QKPELFSKVVLL 134

Query: 209 NCAGG---MNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQRESLRNILLSV 268
            C+ G    ++  I+    I    P   L+              +R   +E +   LL+V
Sbjct: 135 -CSSGYLKRSHPTIIFGTHI----PYFHLY-------------IKRWLSKEGVMKNLLNV 194

Query: 269 YGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVPVLVLWGDEDP 328
             +K  ++E++ID    P  D     A    +    G    + + +++ P L++WG+ED 
Sbjct: 195 VHDKSLIDEEMIDGYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDR 254

Query: 329 FTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWL 376
             P++  +GK    L  +LPN  L+ L   GH   ++RPEL+ E +  ++
Sbjct: 255 IVPME--IGK---RLHADLPNSVLYSLGQTGHLVPEERPELISEHIADFI 272

[Close]

BLAST of Sgr021813 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.7e-08
Identity = 71/265 (26.79%), Postives = 117/265 (44.15%), Query Frame = 0


Query: 110 PVVLLVHGFGASIPHWRRNISTLSQCSTVYAIDLLGFGASDKPVGFAYTMETWANLILDF 169
           P +LL+HG G +   W    + L+Q  TV A DLLG G SDKP    Y++  +AN + D 
Sbjct: 38  PAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRA-DYSVAAYANGMRDL 97

Query: 170 VDQVIKKPTVLVGNSVGSLACVIAASDSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLL- 229
           +  +  +   +VG+S+G    +  A    + LV  L+L++  G   +  IV  +R+  L 
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQ-LVDRLILVSAGGVTKDVNIV--FRLASLP 157

Query: 230 -----LPLLWLFDFLLKQRGIASALFERVKQRESLRNILLSVYGNKESVNEDLIDIIVEP 289
                + LL L   +L    IA  +  +     SL + L +V          ++D + EP
Sbjct: 158 MGSEAMALLRL-PLVLPAVQIAGRIVGKAIGTTSLGHDLPNVL--------RILDDLPEP 217

Query: 290 ANDVGALDAFVSIVTGPPGPSPVQLMPRV----SVPVLVLWGDEDPFTPLDGPVGKYFSN 349
                A    +  V    G   V ++ R     ++PV ++WG +D   P+     ++   
Sbjct: 218 TAS-AAFGRTLRAVVDWRG-QMVTMLDRCYLTEAIPVQIIWGTKDVVLPV-----RHAHM 277

Query: 350 LSMELPNVSLFVLKGVGHCPHDDRP 365
               +P   L + +G GH P  D P
Sbjct: 278 AHAAMPGSQLEIFEGSGHFPFHDDP 282

[Close]

BLAST of Sgr021813 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.7e-08
Identity = 71/265 (26.79%), Postives = 117/265 (44.15%), Query Frame = 0


Query: 110 PVVLLVHGFGASIPHWRRNISTLSQCSTVYAIDLLGFGASDKPVGFAYTMETWANLILDF 169
           P +LL+HG G +   W    + L+Q  TV A DLLG G SDKP    Y++  +AN + D 
Sbjct: 38  PAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRA-DYSVAAYANGMRDL 97

Query: 170 VDQVIKKPTVLVGNSVGSLACVIAASDSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLL- 229
           +  +  +   +VG+S+G    +  A    + LV  L+L++  G   +  IV  +R+  L 
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQ-LVDRLILVSAGGVTKDVNIV--FRLASLP 157

Query: 230 -----LPLLWLFDFLLKQRGIASALFERVKQRESLRNILLSVYGNKESVNEDLIDIIVEP 289
                + LL L   +L    IA  +  +     SL + L +V          ++D + EP
Sbjct: 158 MGSEAMALLRL-PLVLPAVQIAGRIVGKAIGTTSLGHDLPNVL--------RILDDLPEP 217

Query: 290 ANDVGALDAFVSIVTGPPGPSPVQLMPRV----SVPVLVLWGDEDPFTPLDGPVGKYFSN 349
                A    +  V    G   V ++ R     ++PV ++WG +D   P+     ++   
Sbjct: 218 TAS-AAFGRTLRAVVDWRG-QMVTMLDRCYLTEAIPVQIIWGTKDVVLPV-----RHAHM 277

Query: 350 LSMELPNVSLFVLKGVGHCPHDDRP 365
               +P   L + +G GH P  D P
Sbjct: 278 AHAAMPGSQLEIFEGSGHFPFHDDP 282

[Close]

BLAST of Sgr021813 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.7e-08
Identity = 71/265 (26.79%), Postives = 117/265 (44.15%), Query Frame = 0


Query: 110 PVVLLVHGFGASIPHWRRNISTLSQCSTVYAIDLLGFGASDKPVGFAYTMETWANLILDF 169
           P +LL+HG G +   W    + L+Q  TV A DLLG G SDKP    Y++  +AN + D 
Sbjct: 38  PAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRA-DYSVAAYANGMRDL 97

Query: 170 VDQVIKKPTVLVGNSVGSLACVIAASDSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLL- 229
           +  +  +   +VG+S+G    +  A    + LV  L+L++  G   +  IV  +R+  L 
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQ-LVDRLILVSAGGVTKDVNIV--FRLASLP 157

Query: 230 -----LPLLWLFDFLLKQRGIASALFERVKQRESLRNILLSVYGNKESVNEDLIDIIVEP 289
                + LL L   +L    IA  +  +     SL + L +V          ++D + EP
Sbjct: 158 MGSEAMALLRL-PLVLPAVQIAGRIVGKAIGTTSLGHDLPNVL--------RILDDLPEP 217

Query: 290 ANDVGALDAFVSIVTGPPGPSPVQLMPRV----SVPVLVLWGDEDPFTPLDGPVGKYFSN 349
                A    +  V    G   V ++ R     ++PV ++WG +D   P+     ++   
Sbjct: 218 TAS-AAFGRTLRAVVDWRG-QMVTMLDRCYLTEAIPVQIIWGTKDVVLPV-----RHAHM 277

Query: 350 LSMELPNVSLFVLKGVGHCPHDDRP 365
               +P   L + +G GH P  D P
Sbjct: 278 AHAAMPGSQLEIFEGSGHFPFHDDP 282

[Close]

BLAST of Sgr021813 vs. ExPASy TrEMBL
Match: A0A6J1DPH2 (pheophytinase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111022999 PE=4 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 5.9e-190
Identity = 336/368 (91.30%), Postives = 349/368 (94.84%), Query Frame = 0


Query: 16  QVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLSASVGIGAALEVQEI 75
           +V VN QQI+QKKRN FVRH+HRAG FVGLSKN +A AAVITAT SASV IGAALEVQEI
Sbjct: 25  RVDVNFQQIVQKKRNCFVRHSHRAGGFVGLSKNQSAGAAVITATSSASVAIGAALEVQEI 84

Query: 76  RERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQC 135
           RERSNKWQWNQYSIN+FVSHYQPSQQQ QSSSNPP+VLLVHGFGASIPHWRRNISTLSQ 
Sbjct: 85  RERSNKWQWNQYSINYFVSHYQPSQQQSQSSSNPPIVLLVHGFGASIPHWRRNISTLSQY 144

Query: 136 STVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 195
           STVYAIDLLGFGASDKP GFAYTMETWA+LILDFVDQVIKKPTVLVGNSVGSLACVIAAS
Sbjct: 145 STVYAIDLLGFGASDKPAGFAYTMETWAHLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 204

Query: 196 DSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQR 255
           DS RNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLLKQRGIAS LFERVKQR
Sbjct: 205 DSSRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLKQRGIASVLFERVKQR 264

Query: 256 ESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVP 315
           +SLRNILLSVYGNKESV+EDLIDIIVEPAND GALDAFVSIVTGPPGPSPVQLMPRVSVP
Sbjct: 265 DSLRNILLSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPVQLMPRVSVP 324

Query: 316 VLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWL 375
           VL+LWGDEDPFTPLDGPVG+YFS LSME PNVSLFVLKGVGHCPHDDRPELVH+KLLPWL
Sbjct: 325 VLILWGDEDPFTPLDGPVGRYFSKLSMEFPNVSLFVLKGVGHCPHDDRPELVHQKLLPWL 384

Query: 376 AQV--PAL 382
           AQ+  PAL
Sbjct: 385 AQLQRPAL 392

[Close]

BLAST of Sgr021813 vs. ExPASy TrEMBL
Match: A0A6J1EWS0 (pheophytinase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438875 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.8e-184
Identity = 323/366 (88.25%), Postives = 346/366 (94.54%), Query Frame = 0


Query: 16  QVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLSASVGIGAALEVQEI 75
           QVHVN+QQIIQKKRN FVR+++RAGRF GLSKNP+ASAAV+TAT SASVG+GAALEVQEI
Sbjct: 76  QVHVNVQQIIQKKRNLFVRYSNRAGRFFGLSKNPSASAAVVTATSSASVGVGAALEVQEI 135

Query: 76  RERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQC 135
           RERSNKWQWNQYSIN+FVSHYQPSQQQ QSS +PPVVLLVHGFGASIPHWRRNIS LSQ 
Sbjct: 136 RERSNKWQWNQYSINYFVSHYQPSQQQHQSSVDPPVVLLVHGFGASIPHWRRNISALSQY 195

Query: 136 STVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 195
           STVYAIDLLGFGASDKP GFAYTMETWA+LILDFV  +IK+PTVLVGNSVGSLACVIAAS
Sbjct: 196 STVYAIDLLGFGASDKPAGFAYTMETWADLILDFVKDIIKRPTVLVGNSVGSLACVIAAS 255

Query: 196 DSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQR 255
           DS RNLV+GLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLL QR IASALFERVK+R
Sbjct: 256 DSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLNQRRIASALFERVKKR 315

Query: 256 ESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVP 315
           +SLRNIL SVYGNKE+V+EDLIDII+EPAND GALDAFVSIVTGPPGPSPVQLMP++SVP
Sbjct: 316 DSLRNILSSVYGNKENVDEDLIDIILEPANDEGALDAFVSIVTGPPGPSPVQLMPKISVP 375

Query: 316 VLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWL 375
           VL+LWGDEDPFTPLDGPVGKYFS LSME PNVSL VL+GVGHCPHDDRPELVHEKLLPWL
Sbjct: 376 VLILWGDEDPFTPLDGPVGKYFSKLSMEAPNVSLCVLEGVGHCPHDDRPELVHEKLLPWL 435

Query: 376 AQVPAL 382
           AQ+ AL
Sbjct: 436 AQLHAL 441

[Close]

BLAST of Sgr021813 vs. ExPASy TrEMBL
Match: A0A5A7U2I7 (Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold155G001000 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 7.0e-183
Identity = 325/380 (85.53%), Postives = 350/380 (92.11%), Query Frame = 0


Query: 3   IRVTGPHSGFVWLQVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLS- 62
           +RV GPHSGF+WLQV VNLQQ   + +N F+R  + AGRF+GLSKNP+  AAVITA  S 
Sbjct: 1   MRVMGPHSGFIWLQVLVNLQQTFLRNQNCFIRCRNWAGRFIGLSKNPSTKAAVITAASSA 60

Query: 63  ASVGIGAALEVQEIRERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGAS 122
           ASV IG ALEVQEIRERSNKWQWNQYSIN+FVSHYQP QQQPQSSS+ PVVLLVHGFGAS
Sbjct: 61  ASVCIGPALEVQEIRERSNKWQWNQYSINYFVSHYQP-QQQPQSSSS-PVVLLVHGFGAS 120

Query: 123 IPHWRRNISTLSQCSTVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLV 182
           IPHWRRNISTLSQ STVYAIDLLGFGASDKP GFAYTMETWA L++DFVDQ+IKKPTVLV
Sbjct: 121 IPHWRRNISTLSQYSTVYAIDLLGFGASDKPAGFAYTMETWAELVIDFVDQIIKKPTVLV 180

Query: 183 GNSVGSLACVIAASDSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQ 242
           GNSVGSLACVIAASDS RNLV+GLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLL Q
Sbjct: 181 GNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLNQ 240

Query: 243 RGIASALFERVKQRESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPP 302
           R IASALF+RVK+R+SLRNIL SVYGNKESV+EDLIDIIVEPAND GALDAFVSIVTGPP
Sbjct: 241 RIIASALFKRVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPP 300

Query: 303 GPSPVQLMPRVSVPVLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHD 362
           GPSPV+LMP++SVPVL+LWGD+DPFTPLDGPVGKYF  LSME+PNVSLFVLKGVGHCPHD
Sbjct: 301 GPSPVELMPKLSVPVLILWGDKDPFTPLDGPVGKYFRKLSMEVPNVSLFVLKGVGHCPHD 360

Query: 363 DRPELVHEKLLPWLAQVPAL 382
           DRPELVHEKLLPWLAQ+PAL
Sbjct: 361 DRPELVHEKLLPWLAQLPAL 378

[Close]

BLAST of Sgr021813 vs. ExPASy TrEMBL
Match: A0A6J1ID25 (pheophytinase, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471988 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.6e-182
Identity = 322/366 (87.98%), Postives = 344/366 (93.99%), Query Frame = 0


Query: 16  QVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLSASVGIGAALEVQEI 75
           QVHVNLQQIIQKKRN FVR+++RAGRF GLSKNP+ASAAVI+AT SA VGIGAALEVQEI
Sbjct: 76  QVHVNLQQIIQKKRNRFVRYSNRAGRFFGLSKNPSASAAVISATSSAYVGIGAALEVQEI 135

Query: 76  RERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQC 135
           RERSNKWQWNQYSIN+FVSHYQPSQQ+ QSS +PPVVLLVHGFGASIPHWRRNIS LSQ 
Sbjct: 136 RERSNKWQWNQYSINYFVSHYQPSQQEHQSSVDPPVVLLVHGFGASIPHWRRNISALSQY 195

Query: 136 STVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAAS 195
           STVYAIDLLGFGASDKP GFAYTMETWA+LILDFV  +IK+PTVLVGNSVGSLACVIAAS
Sbjct: 196 STVYAIDLLGFGASDKPAGFAYTMETWADLILDFVKDIIKRPTVLVGNSVGSLACVIAAS 255

Query: 196 DSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQR 255
           DS RNLV+GLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLL QR IASALFERVK+R
Sbjct: 256 DSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLNQRRIASALFERVKKR 315

Query: 256 ESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVP 315
           +SLR+IL SVYGNKE+V+EDLIDII EPAND GALDAFVSIVTGPPGPSPVQLMP++SVP
Sbjct: 316 DSLRDILSSVYGNKENVDEDLIDIIFEPANDEGALDAFVSIVTGPPGPSPVQLMPKISVP 375

Query: 316 VLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWL 375
           VL+LWGDEDPFTPLDGPVGKYFS LS+E PNVSL VLKGVGHCPHDDRPELVHEKLLPWL
Sbjct: 376 VLILWGDEDPFTPLDGPVGKYFSKLSVESPNVSLCVLKGVGHCPHDDRPELVHEKLLPWL 435

Query: 376 AQVPAL 382
           AQ+ AL
Sbjct: 436 AQLHAL 441

[Close]

BLAST of Sgr021813 vs. ExPASy TrEMBL
Match: A0A0A0KAI8 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014690 PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.7e-176
Identity = 320/367 (87.19%), Postives = 337/367 (91.83%), Query Frame = 0


Query: 16  QVHVNLQQIIQKKRNSFVRHTHRAGRFVGLSKNPTASAAVITATLS-ASVGIGAALEVQE 75
           +VHVNLQQ  Q+ RN  +R  + AGRFVGLSKNP+  AAVITAT S ASV IG ALEVQE
Sbjct: 65  RVHVNLQQTFQRNRNCLIRSRNWAGRFVGLSKNPSTKAAVITATSSAASVCIGPALEVQE 124

Query: 76  IRERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQ 135
           IRERSNKWQWNQYSIN+FVS YQ  QQQPQS S+ PVVLLVHGFGASIPHWRRNISTLSQ
Sbjct: 125 IRERSNKWQWNQYSINYFVSDYQ-LQQQPQSRSS-PVVLLVHGFGASIPHWRRNISTLSQ 184

Query: 136 CSTVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAA 195
            STVYAIDLLGFGASDKP GFAYTMETWA LILDFVDQVIKKPTVLVGNSVGSLACVIAA
Sbjct: 185 YSTVYAIDLLGFGASDKPAGFAYTMETWAELILDFVDQVIKKPTVLVGNSVGSLACVIAA 244

Query: 196 SDSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQ 255
           SDS RNLV+GLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLL QR IASALFERVK+
Sbjct: 245 SDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRIIASALFERVKK 304

Query: 256 RESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSV 315
           R+SLRNIL SVYGNKESV+EDLIDIIVEPAND GALDAFVSIVTGPPGPSPV+LMP++SV
Sbjct: 305 RDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPVELMPKISV 364

Query: 316 PVLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPW 375
           PVL+LWGDEDPFTPLDGPVGKYF  LSME+ NVSLFVLKGVGHCPHDDRPELVHEKLLPW
Sbjct: 365 PVLILWGDEDPFTPLDGPVGKYFKKLSMEVSNVSLFVLKGVGHCPHDDRPELVHEKLLPW 424

Query: 376 LAQVPAL 382
           LAQ+PAL
Sbjct: 425 LAQLPAL 429

[Close]

BLAST of Sgr021813 vs. TAIR 10
Match: AT5G38520.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 458.4 bits (1178), Expect = 5.7e-129
Identity = 223/326 (68.40%), Postives = 277/326 (84.97%), Query Frame = 0


Query: 57  TATLSASV---GIGAALEVQEIRERSNKWQW-NQYSINFFVSHYQPSQQQPQSSSNPPVV 116
           TA+ SA+V   G+  A+E+ EI ERS KW+W  +YS+N+FV    P +  P S +    V
Sbjct: 39  TASSSATVSGGGVVEAVELAEIGERSKKWKWKGEYSVNYFVKD-SPEEVTPASQT----V 98

Query: 117 LLVHGFGASIPHWRRNISTLSQCSTVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQ 176
           LLVHGFGASIPHWRRNI+ LS+  TVYAIDLLGFGASDKP GF+YTME+WA LIL+F+++
Sbjct: 99  LLVHGFGASIPHWRRNINALSKNHTVYAIDLLGFGASDKPPGFSYTMESWAELILNFLEE 158

Query: 177 VIKKPTVLVGNSVGSLACVIAASDSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLL 236
           V++KPT+L+GNSVGSLACVIAAS+SR +LV+GLVLLNCAGGMNNKA+ DDWRIKLL+PLL
Sbjct: 159 VVQKPTILIGNSVGSLACVIAASESRGDLVKGLVLLNCAGGMNNKAVFDDWRIKLLMPLL 218

Query: 237 WLFDFLLKQRGIASALFERVKQRESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDA 296
            L DFLLKQRGIASALF RVK RE+L+NIL +VYGNK++V++ L++II  PAN  GALDA
Sbjct: 219 LLIDFLLKQRGIASALFNRVKDRENLKNILTNVYGNKDNVDDTLVEIIAGPANTEGALDA 278

Query: 297 FVSIVTGPPGPSPVQLMPRVSVPVLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVL 356
           FVSI+TGPPGP+P++L+P ++ PVLVLWGD+D  TPLDGPVGKYF++L  +LPN +L+VL
Sbjct: 279 FVSILTGPPGPNPIKLIPEITKPVLVLWGDQDGLTPLDGPVGKYFTSLPDQLPNFNLYVL 338

Query: 357 KGVGHCPHDDRPELVHEKLLPWLAQV 379
           +GVGHCP DDRP+LVHE+LLPWLAQ+
Sbjct: 339 QGVGHCPQDDRPDLVHERLLPWLAQL 359

[Close]

BLAST of Sgr021813 vs. TAIR 10
Match: AT5G38520.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 449.5 bits (1155), Expect = 2.7e-126
Identity = 223/338 (65.98%), Postives = 277/338 (81.95%), Query Frame = 0


Query: 57  TATLSASV---GIGAALEVQEIRERSNKWQW-NQYSINFFVSHYQPSQQQPQSSSNPPVV 116
           TA+ SA+V   G+  A+E+ EI ERS KW+W  +YS+N+FV    P +  P S +    V
Sbjct: 39  TASSSATVSGGGVVEAVELAEIGERSKKWKWKGEYSVNYFVKD-SPEEVTPASQT----V 98

Query: 117 LLVHGFGASIPHWRRNISTLSQCSTVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQ 176
           LLVHGFGASIPHWRRNI+ LS+  TVYAIDLLGFGASDKP GF+YTME+WA LIL+F+++
Sbjct: 99  LLVHGFGASIPHWRRNINALSKNHTVYAIDLLGFGASDKPPGFSYTMESWAELILNFLEE 158

Query: 177 VIKKPTVLVGNSVGSLACVIAAS------------DSRRNLVQGLVLLNCAGGMNNKAIV 236
           V++KPT+L+GNSVGSLACVIAAS            +SR +LV+GLVLLNCAGGMNNKA+ 
Sbjct: 159 VVQKPTILIGNSVGSLACVIAASGTKFLIYLEKKTESRGDLVKGLVLLNCAGGMNNKAVF 218

Query: 237 DDWRIKLLLPLLWLFDFLLKQRGIASALFERVKQRESLRNILLSVYGNKESVNEDLIDII 296
           DDWRIKLL+PLL L DFLLKQRGIASALF RVK RE+L+NIL +VYGNK++V++ L++II
Sbjct: 219 DDWRIKLLMPLLLLIDFLLKQRGIASALFNRVKDRENLKNILTNVYGNKDNVDDTLVEII 278

Query: 297 VEPANDVGALDAFVSIVTGPPGPSPVQLMPRVSVPVLVLWGDEDPFTPLDGPVGKYFSNL 356
             PAN  GALDAFVSI+TGPPGP+P++L+P ++ PVLVLWGD+D  TPLDGPVGKYF++L
Sbjct: 279 AGPANTEGALDAFVSILTGPPGPNPIKLIPEITKPVLVLWGDQDGLTPLDGPVGKYFTSL 338

Query: 357 SMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQV 379
             +LPN +L+VL+GVGHCP DDRP+LVHE+LLPWLAQ+
Sbjct: 339 PDQLPNFNLYVLQGVGHCPQDDRPDLVHERLLPWLAQL 371

[Close]

BLAST of Sgr021813 vs. TAIR 10
Match: AT5G19850.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 2.4e-42
Identity = 96/311 (30.87%), Postives = 167/311 (53.70%), Query Frame = 0


Query: 73  QEIRERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTL 132
           +E++ R+  W+W  YSI +             + ++ P ++LVHGFGA+  HWR+N   L
Sbjct: 69  EELQVRTLTWKWKGYSIRY-----------QCAGTSGPALVLVHGFGANSDHWRKNTPIL 128

Query: 133 SQCSTVYAIDLLGFGASDKPVGFA------YTMETWANLILDFVDQVIKKPTVLVGNSVG 192
            +   VY+IDL+G+G SDKP          YT ETW   + DF   V+K     + NS+G
Sbjct: 129 GKTHRVYSIDLIGYGYSDKPNPREFGGEPFYTFETWGEQLNDFCLDVVKDEAFFICNSIG 188

Query: 193 SLACVIAASDSRRNLVQGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLKQRGIAS 252
            L  + AA  S+  + +GL+L+N    ++ + +    +  +  P +  F  LL+   +  
Sbjct: 189 GLVGLQAAV-SKPEICRGLMLIN----ISLRMLHIKKQPFIGRPFIKSFQNLLRNTPVGK 248

Query: 253 ALFERVKQRESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSIVTGPPGPSPV 312
             F+ + + E++++IL   Y +   V ++L++ I+ P  + GA+D F+  +    GP P 
Sbjct: 249 LFFKSIAKPETVKSILCQCYHDSSQVTDELVEAILRPGLEPGAVDVFLEFICYSGGPLPE 308

Query: 313 QLMPRVSVPVLVLWGDEDPFTPLDGPVGKYFSNLSMELPNVSLFVLKGVGHCPHDDRPEL 372
            L+P V  PVL+ WG++DP+ P++  +G+ +SN           VL   GHCP D++PE+
Sbjct: 309 DLLPLVKCPVLIAWGEKDPWEPIE--LGRAYSNFDAV---EDFVVLPDAGHCPQDEKPEM 358

Query: 373 VHEKLLPWLAQ 378
           V+  +  ++A+
Sbjct: 369 VNPLIESFVAR 358

[Close]

BLAST of Sgr021813 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 1.8e-37
Identity = 103/315 (32.70%), Postives = 166/315 (52.70%), Query Frame = 0


Query: 77  ERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQCS 136
           E  N W+W  + I++ V          Q   +P  ++L+HGFGAS+ HWR NI  L++  
Sbjct: 22  EGYNFWEWRGHKIHYVV----------QGEGSP--LVLIHGFGASVFHWRYNIPELAKKY 81

Query: 137 TVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAASD 196
            VYA+DLLGFG SDK +   Y    W + ++DF+ +V+K+P V+VGNS+G    +  A  
Sbjct: 82  KVYALDLLGFGWSDKAL-IEYDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVG 141

Query: 197 SRRNLVQGLVLLNCAGGM----NNKAIVDDWRIK--LLLPLLWLFDFLLKQRGIASALFE 256
                V G+ LLN AG        +   D+  I   ++ PL  +F     QR +   LF 
Sbjct: 142 LPEQ-VTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKEIF-----QRVVLGFLFW 201

Query: 257 RVKQRESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSI----VTGPPGPSPV 316
           + KQ   + ++L SVY +  +V++ L++ I +PA D  A + +  +    +T     +  
Sbjct: 202 QAKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFLTNQSRYTLD 261

Query: 317 QLMPRVSVPVLVLWGDEDPFTPLDGP-----VGKYFSNLSMELPNVSLFVLKGVGHCPHD 376
            ++ +++ P+L++WGD DP+    GP     +  ++SN S+        V    GHCPHD
Sbjct: 262 SVLSKMTCPLLLVWGDLDPWV---GPAKAEKIKAFYSNSSL--------VHLQAGHCPHD 306

[Close]

BLAST of Sgr021813 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 1.8e-37
Identity = 103/315 (32.70%), Postives = 166/315 (52.70%), Query Frame = 0


Query: 77  ERSNKWQWNQYSINFFVSHYQPSQQQPQSSSNPPVVLLVHGFGASIPHWRRNISTLSQCS 136
           E  N W+W  + I++ V          Q   +P  ++L+HGFGAS+ HWR NI  L++  
Sbjct: 79  EGYNFWEWRGHKIHYVV----------QGEGSP--LVLIHGFGASVFHWRYNIPELAKKY 138

Query: 137 TVYAIDLLGFGASDKPVGFAYTMETWANLILDFVDQVIKKPTVLVGNSVGSLACVIAASD 196
            VYA+DLLGFG SDK +   Y    W + ++DF+ +V+K+P V+VGNS+G    +  A  
Sbjct: 139 KVYALDLLGFGWSDKAL-IEYDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVG 198

Query: 197 SRRNLVQGLVLLNCAGGM----NNKAIVDDWRIK--LLLPLLWLFDFLLKQRGIASALFE 256
                V G+ LLN AG        +   D+  I   ++ PL  +F     QR +   LF 
Sbjct: 199 LPEQ-VTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKEIF-----QRVVLGFLFW 258

Query: 257 RVKQRESLRNILLSVYGNKESVNEDLIDIIVEPANDVGALDAFVSI----VTGPPGPSPV 316
           + KQ   + ++L SVY +  +V++ L++ I +PA D  A + +  +    +T     +  
Sbjct: 259 QAKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFLTNQSRYTLD 318

Query: 317 QLMPRVSVPVLVLWGDEDPFTPLDGP-----VGKYFSNLSMELPNVSLFVLKGVGHCPHD 376
            ++ +++ P+L++WGD DP+    GP     +  ++SN S+        V    GHCPHD
Sbjct: 319 SVLSKMTCPLLLVWGDLDPWV---GPAKAEKIKAFYSNSSL--------VHLQAGHCPHD 363

The following BLAST results are available for this feature:

BLAST of Sgr021813 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156028.1	1.2e-189	91.30	pheophytinase, chloroplastic [Momordica charantia]	[more]
XP_038884599.1	1.4e-185	89.07	pheophytinase, chloroplastic isoform X1 [Benincasa hispida]	[more]
XP_023523832.1	1.5e-184	88.80	pheophytinase, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022932467.1	5.8e-184	88.25	pheophytinase, chloroplastic [Cucurbita moschata] >XP_022932468.1 pheophytinase,...	[more]
KAA0047761.1	1.4e-182	85.53	pheophytinase [Cucumis melo var. makuwa] >TYK02393.1 pheophytinase [Cucumis melo...	[more]

BLAST of Sgr021813 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FFZ1	2.5e-20	26.89	Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1	[more]
O05235	3.3e-20	27.24	Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yu...	[more]
P9WNH2	1.7e-08	26.79	Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ...	[more]
P9WNH3	1.7e-08	26.79	Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 ...	[more]
P0A573	1.7e-08	26.79	Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2...	[more]

BLAST of Sgr021813 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DPH2	5.9e-190	91.30	pheophytinase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111022999 PE=4...	[more]
A0A6J1EWS0	2.8e-184	88.25	pheophytinase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438875 PE=4 ...	[more]
A0A5A7U2I7	7.0e-183	85.53	Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold155G001000...	[more]
A0A6J1ID25	1.6e-182	87.98	pheophytinase, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471...	[more]
A0A0A0KAI8	1.7e-176	87.19	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014...	[more]

BLAST of Sgr021813 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G38520.1	5.7e-129	68.40	alpha/beta-Hydrolases superfamily protein	[more]
AT5G38520.2	2.7e-126	65.98	alpha/beta-Hydrolases superfamily protein	[more]
AT5G19850.1	2.4e-42	30.87	alpha/beta-Hydrolases superfamily protein	[more]
AT4G36530.1	1.8e-37	32.70	alpha/beta-Hydrolases superfamily protein	[more]
AT4G36530.2	1.8e-37	32.70	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 380..381
None	No IPR available	PANTHER	PTHR46438:SF7	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 29..380
None	No IPR available	PANTHER	PTHR46438	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 29..380
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF12697	Abhydrolase_6	coord: 112..368 e-value: 3.3E-19 score: 70.4
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 72..377 e-value: 2.2E-50 score: 173.9
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 80..375

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr021813.1	Sgr021813.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015994	chlorophyll metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0047746	chlorophyllase activity