Homology
BLAST of Sgr021601 vs. NCBI nr
Match:
XP_022158049.1 (probable pectinesterase/pectinesterase inhibitor 61 [Momordica charantia])
HSP 1 Score: 993.4 bits (2567), Expect = 8.4e-286
Identity = 505/606 (83.33%), Postives = 545/606 (89.93%), Query Frame = 0
Query: 1 MNYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAV 60
MNYGKLHS++SGD TP NF LS+ S+ P+ R TRVVLLS LS+LLI ASA+SAV
Sbjct: 1 MNYGKLHSTSSGD-TPANFELSNSNSSI--SSPNRRTRTRVVLLSLLSILLIMASAISAV 60
Query: 61 LLLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHIS 120
+ +R I G G KP+QAISRACSRTLYPA+CVSSL++FPGAL+ADVHDLVHIS
Sbjct: 61 VFVRSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHIS 120
Query: 121 LNVTLQRLTKALYSSSQISASQISDPLALSAYEDCMELLDDAVDAFSRSLLSIVPSSDTN 180
LNVTLQRLTKALYSSSQI SQISDPL LSA+ DC+ELLDDAVDAFSRSL S S N
Sbjct: 121 LNVTLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAAS---N 180
Query: 181 HDVLTWLSAALTYQDTCTEGLADVS-GPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDD 240
HDVLTWLSAALTY DTCTEGLADVS G VKDQMT L DLSQLISNSLAIFSGFGGGT +
Sbjct: 181 HDVLTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGE 240
Query: 241 FSGVPIQNKRRLMQSQWPPENAAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAKDGTG 300
FSGVP+QN+RRLM+++WP EN GE+FP WVSRKDRQLL SPIS+IQADIVVAKDGTG
Sbjct: 241 FSGVPVQNRRRLMEAEWPQENG---GEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 300
Query: 301 NYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 360
YKTVAEAI AAPSSSGRRT+IY+KAG+Y EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 301 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 360
Query: 361 IYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIGYQD 420
++DKVTTFRTATFAASGTG+ILRDMTFEN+AGP +HQAVALR+SADHAVVFRC++IGYQD
Sbjct: 361 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 420
Query: 421 TLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQN 480
TLYVHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQDRKDPNQN
Sbjct: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 480
Query: 481 TGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 540
TGISIHAC+ILA SDLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 481 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 540
Query: 541 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGVSFS 600
ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITST+EA KFTVGQFIYGSAWLPSTGVSFS
Sbjct: 541 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFS 597
Query: 601 AGLSAN 606
AGLSA+
Sbjct: 601 AGLSAS 597
BLAST of Sgr021601 vs. NCBI nr
Match:
XP_022957644.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])
HSP 1 Score: 929.1 bits (2400), Expect = 2.0e-266
Identity = 480/609 (78.82%), Postives = 532/609 (87.36%), Query Frame = 0
Query: 2 NYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAVL 61
NY KLHSS SGD TP F +SH P PP +SRR TR++ +S +S+LLI ASAVSA +
Sbjct: 3 NYNKLHSSASGD-TPATFQVSHTP--NPPSSSASRRRTRILFVSLISILLITASAVSAAV 62
Query: 62 LLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHISL 121
LL + S F KPTQAIS ACS TLYP+LC SSLL FPG+LSADVH LVHISL
Sbjct: 63 LLHSQSSHFPHN------KPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISL 122
Query: 122 NVTLQRLTKALYSSSQISASQIS-DPLALSAYEDCMELLDDAVDAFSRSLLSIVPSSDTN 181
N+TLQ LTKALY+SSQI QIS +PLA SAY+DC+ELL+DAVDAFS SL S +S +N
Sbjct: 123 NLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFS---TSASN 182
Query: 182 HDVLTWLSAALTYQDTCTEGLADVSGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDDF 241
HD++TWLSAALTY DTC++G ADV PVKDQM L+DLS++ISNSLAIFSG+G
Sbjct: 183 HDIMTWLSAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYG------ 242
Query: 242 SGVPIQNKRRLMQS--QWPPEN--AAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAKD 301
G+P+QN+RRLM+S +W EN A AEGE FP WVS KDR+LLA+PIS+IQADIVVAKD
Sbjct: 243 EGLPVQNRRRLMESDREWAAENGVAVAEGE-FPGWVSGKDRRLLAAPISAIQADIVVAKD 302
Query: 302 GTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISG 361
G+G +KTVAEAIEAAPSSSGRR +IYIKAG+YEEENLKVGRKKTNLMFVGDGKGITVISG
Sbjct: 303 GSGKFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISG 362
Query: 362 GKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIG 421
GKS++DKVTTFRTATFA SGTG+ILRDMTFEN+AGPSKHQAVALR+SADHAVV+RC++IG
Sbjct: 363 GKSVFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIG 422
Query: 422 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDP 481
YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+RKDP
Sbjct: 423 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDP 482
Query: 482 NQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ 541
NQNTGISIHAC+ILAT+DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ
Sbjct: 483 NQNTGISIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ 542
Query: 542 GSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGV 601
GSFALDTLYYGEYMNSGPGAA GQRV WPGYRVITSTIEA+KFTVGQFIYGS+WLPSTGV
Sbjct: 543 GSFALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGV 592
Query: 602 SFSAGLSAN 606
SFSAGLSAN
Sbjct: 603 SFSAGLSAN 592
BLAST of Sgr021601 vs. NCBI nr
Match:
XP_023533011.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 923.7 bits (2386), Expect = 8.2e-265
Identity = 477/609 (78.33%), Postives = 529/609 (86.86%), Query Frame = 0
Query: 2 NYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAVL 61
NY KLHSS SGD TP F +SH P PP +SRR TR++ +S +S+LLI ASAVSA +
Sbjct: 3 NYNKLHSSGSGD-TPATFQVSHTP--NPPSSSASRRRTRILFVSLISILLITASAVSAAV 62
Query: 62 LLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHISL 121
L+ S F KPTQAIS ACS TLYP+LC SSLL FPG+LSADVH LVHISL
Sbjct: 63 LIHTNTSHFPHN------KPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHHLVHISL 122
Query: 122 NVTLQRLTKALYSSSQISASQIS-DPLALSAYEDCMELLDDAVDAFSRSLLSIVPSSDTN 181
N+TLQ LTKALY+SSQI QIS +PLA SAY+DC+ELL+DAVDAFS SL S +S +N
Sbjct: 123 NLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFS---TSASN 182
Query: 182 HDVLTWLSAALTYQDTCTEGLADVSGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDDF 241
HD++TWLSAALTY DTC++G ADV PVKDQM L+DLS++ISNSLAIFSG+G
Sbjct: 183 HDIMTWLSAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYG------ 242
Query: 242 SGVPIQNKRRLMQ--SQWPPEN--AAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAKD 301
G+P+QN+RRLM+ S+W EN A AEGE FP WVS KDR+LLA+PIS+IQADIVVAKD
Sbjct: 243 QGLPVQNRRRLMESDSEWAAENGVAVAEGE-FPGWVSGKDRRLLAAPISAIQADIVVAKD 302
Query: 302 GTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISG 361
G+G + TVAEAIEAAPSSSGRR +IYIKAG+YEEENLKVGRKKTNLMFVGDGKGITVISG
Sbjct: 303 GSGKFTTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISG 362
Query: 362 GKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIG 421
GKS++DKVTTFRTATFA SGTG+ILRDMTFEN+AGPSKHQAVALR+SADHAVV+RC++IG
Sbjct: 363 GKSVFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIG 422
Query: 422 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDP 481
YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+RKDP
Sbjct: 423 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDP 482
Query: 482 NQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ 541
NQNTGISIHAC+ILAT+DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ
Sbjct: 483 NQNTGISIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ 542
Query: 542 GSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGV 601
GSFALDTLYYGEYMNSGPGAA GQRV WPGYRVITSTIEA+KFTVGQFIYGS+WLPSTGV
Sbjct: 543 GSFALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGV 592
Query: 602 SFSAGLSAN 606
SFSAGLS N
Sbjct: 603 SFSAGLSTN 592
BLAST of Sgr021601 vs. NCBI nr
Match:
XP_022972980.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita maxima])
HSP 1 Score: 923.3 bits (2385), Expect = 1.1e-264
Identity = 475/609 (78.00%), Postives = 529/609 (86.86%), Query Frame = 0
Query: 2 NYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAVL 61
NY KLHSS SGD T+F +S++ + PPPPP S R++L+SF S+LLI AS VSAV+
Sbjct: 4 NYNKLHSSRSGDTPVTSFRVSNDHSNPPPPPPPSSSHRRILLISFFSILLITASTVSAVV 63
Query: 62 LLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHISL 121
L+ RI+ H KPTQAISRACS TLYP+LC+SSLL PG+LSADVHDLVHISL
Sbjct: 64 LVHSRITT-STESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDLVHISL 123
Query: 122 NVTLQRLTKALYSSSQISASQIS-DPLALSAYEDCMELLDDAVDAFSRSLLSIVPSSDTN 181
N+TLQ LTKALYS+SQI SQIS DPLA SAYEDCMELL+DAV+AFS SL S ++ TN
Sbjct: 124 NLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFS---TTATN 183
Query: 182 HDVLTWLSAALTYQDTCTEGLADVSGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDDF 241
HDV+TWLSAALTY DTCT+G +DV+ PVKDQ+T L+DLS++ISNSLAIFSGFGGG
Sbjct: 184 HDVMTWLSAALTYHDTCTDGFSDVADPVKDQITAKLSDLSEMISNSLAIFSGFGGG---- 243
Query: 242 SGVPIQN--KRRLMQS--QWPPENAAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAKD 301
+P++N +RRLM+S QW E +G DFPRWVS KDR+LLA+PISSIQADIVVAKD
Sbjct: 244 -DLPVENRRRRRLMESDEQWLAEENNGDG-DFPRWVSGKDRRLLAAPISSIQADIVVAKD 303
Query: 302 GTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISG 361
G+G +KTVAEAI AAPSSS RR +IYIKAG+YEEENLKVGRKKTNLMFVGDGKGITVISG
Sbjct: 304 GSGKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISG 363
Query: 362 GKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIG 421
GKS+YDKVTTFRTATFA SGTG+ILRDMTFEN+AGP+KHQAVALRLSADH VVFR +VIG
Sbjct: 364 GKSVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIG 423
Query: 422 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDP 481
YQDTLYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM QKNTITAQDRKDP
Sbjct: 424 YQDTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDP 483
Query: 482 NQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ 541
NQNTGISIHAC+I+AT DL+ASKGSFPT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQ
Sbjct: 484 NQNTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQ 543
Query: 542 GSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGV 601
GSFALDTLYYGEYMNSGP AAVGQRVKWPGYRVITS IEA+KFTVGQFIYGS+WLPSTGV
Sbjct: 544 GSFALDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGV 602
Query: 602 SFSAGLSAN 606
SFSAGLS N
Sbjct: 604 SFSAGLSTN 602
BLAST of Sgr021601 vs. NCBI nr
Match:
KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 922.2 bits (2382), Expect = 2.4e-264
Identity = 481/610 (78.85%), Postives = 535/610 (87.70%), Query Frame = 0
Query: 2 NYGKLHSSTSGDNTPTNFHLSHEPIS-LPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAV 61
NY KLHSS SGD ++F +S++ LPPP PSS R R++L+SFLS+ LI ASAVSAV
Sbjct: 4 NYNKLHSSRSGDTPASSFRVSNDHSDPLPPPSPSSSR-RRILLISFLSIFLITASAVSAV 63
Query: 62 LLLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHIS 121
+L+ RI+ H KPTQAISRACS TLYP+LC+SSLL PG+LSADVHDLVHIS
Sbjct: 64 VLVHSRIA-ASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHIS 123
Query: 122 LNVTLQRLTKALYSSSQISASQIS-DPLALSAYEDCMELLDDAVDAFSRSLLSIVPSSDT 181
LN+TLQ LTKALYS+SQI SQIS DPLA SAYEDCMELL+DAV+AFS SL S ++ T
Sbjct: 124 LNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFS---TTAT 183
Query: 182 NHDVLTWLSAALTYQDTCTEGLADVSGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDD 241
NHDV+TWLSAALTY DTCT+G +DV+ P+KDQ+T L+DLS++ISNSLAIFSGFGG DD
Sbjct: 184 NHDVMTWLSAALTYHDTCTDGFSDVADPIKDQITAKLSDLSEMISNSLAIFSGFGG--DD 243
Query: 242 FSGVPIQN--KRRLMQS--QWPPENAAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAK 301
+P++N +RRLM+S W E +G DFP+WVS KDR+LLA+PISSIQADIVVAK
Sbjct: 244 ---LPVENRRRRRLMESDEHWLAEENNGDG-DFPQWVSGKDRRLLAAPISSIQADIVVAK 303
Query: 302 DGTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVIS 361
DG+G +KTVAEAI AAPSSSGRR +IYIKAG+YEEENLKVGRKKTNLMFVGDGKGITVIS
Sbjct: 304 DGSGKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVIS 363
Query: 362 GGKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVI 421
GGKS+YDKVTTFRTATFA SGTG+ILRDMTFEN+AGP+KHQAVALRLSADHAVVFR +VI
Sbjct: 364 GGKSVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVI 423
Query: 422 GYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKD 481
GYQDTLYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM QKNTITAQDRKD
Sbjct: 424 GYQDTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKD 483
Query: 482 PNQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEW 541
PNQNTGISIHAC+I+AT DLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEW
Sbjct: 484 PNQNTGISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEW 543
Query: 542 QGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTG 601
QGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEA+KFTVGQFIYGS+WLPSTG
Sbjct: 544 QGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEATKFTVGQFIYGSSWLPSTG 602
Query: 602 VSFSAGLSAN 606
VSFSAGLS N
Sbjct: 604 VSFSAGLSTN 602
BLAST of Sgr021601 vs. ExPASy Swiss-Prot
Match:
Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)
HSP 1 Score: 738.4 bits (1905), Expect = 6.4e-212
Identity = 377/608 (62.01%), Postives = 466/608 (76.64%), Query Frame = 0
Query: 1 MNYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAV 60
M Y +L G + P+N + SL P ++ T+ L+ F +L ++ V
Sbjct: 1 MGYDRL-----GPSGPSNPNQKDPATSL----PELQKKTKTKLILF-TLAVLVVGVVCFG 60
Query: 61 LLLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHIS 120
+ R D G+ KPTQAISR CS++LYP LC+ +LLDFPG+L+AD ++L+HIS
Sbjct: 61 IFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHIS 120
Query: 121 LNVTLQRLTKALYSSSQISASQISDPLALSAYEDCMELLDDAVDAFSRSLLS-IVPSSDT 180
N TLQ+ +KALY+SS I+ +Q+ P SAY+ C+ELLDD+VDA +R+L S +V S D
Sbjct: 121 FNATLQKFSKALYTSSTITYTQM-PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE 180
Query: 181 NH-DVLTWLSAALTYQDTCTEGLADV---SGPVKDQMTTTLNDLSQLISNSLAIFSGFGG 240
+H DV+TWLS+A+T DTCT+G ++ G VKDQ+ + DLS+++SN LAIF+ G
Sbjct: 181 SHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFA---G 240
Query: 241 GTDDFSGVPIQNKRRLMQSQWPPENAAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAK 300
D SGVP+ N R+L+ ++ E E+ P W+ R+DR+LL +P S+IQADI V+K
Sbjct: 241 KVKDLSGVPVVNNRKLLGTE--------ETEELPNWLKREDRELLGTPTSAIQADITVSK 300
Query: 301 DGTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVIS 360
DG+G +KT+AEAI+ AP S RR +IY+KAGRYEEENLKVGRKKTNLMF+GDGKG TVI+
Sbjct: 301 DGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVIT 360
Query: 361 GGKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVI 420
GGKSI D +TTF TATFAA+G G I+RDMTFEN AGP+KHQAVALR+ DHAVV+RC++I
Sbjct: 361 GGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNII 420
Query: 421 GYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKD 480
GYQD LYVHSNRQFFREC+IYGTVDFIFGNA V+ QSCNIYARKPM QQK TITAQ+RKD
Sbjct: 421 GYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKD 480
Query: 481 PNQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEW 540
PNQNTGISIHAC++LAT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW
Sbjct: 481 PNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEW 540
Query: 541 QGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTG 600
G FALD+LYYGEYMN G G+ +GQRVKWPGY VITST+EASKFTV QFI GS+WLPSTG
Sbjct: 541 NGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTG 586
Query: 601 VSFSAGLS 604
VSF +GLS
Sbjct: 601 VSFFSGLS 586
BLAST of Sgr021601 vs. ExPASy Swiss-Prot
Match:
Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)
HSP 1 Score: 709.9 bits (1831), Expect = 2.4e-203
Identity = 365/577 (63.26%), Postives = 446/577 (77.30%), Query Frame = 0
Query: 31 PPPSSRRSTRVVLLS--FLSLLLIAASAVSAVLLLRHRISDFGRGGHAFPLKPTQAISRA 90
P ++RRS + +++S L++ LI A+A+ A + R +++ G KP+QAIS+A
Sbjct: 34 PENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPG---LARKPSQAISKA 93
Query: 91 CSRTLYPALCVSSLLDFPGALSA-DVHDLVHISLNVTLQRLTKALYSSSQISASQISDPL 150
C T +P LCV SL+DFPG+L+A DL+H+++N+TL + ALYSS+ +S + P
Sbjct: 94 CELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDM-PPR 153
Query: 151 ALSAYEDCMELLDDAVDAFSRSLLSIVPSSDTNHDVLTWLSAALTYQDTCTEGLADV-SG 210
A SAY+ C+ELLDD+VDA SR+L S+V SS DV TWLSAALT DTCTEG V G
Sbjct: 154 ARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTNHDTCTEGFDGVDDG 213
Query: 211 PVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDDFSGVPIQNKRRLMQSQWPPENAAAEGE 270
VKD MT L +LS+L+SN LAIFS G DDF+GVPIQN+R L E
Sbjct: 214 GVKDHMTAALQNLSELVSNCLAIFSASHDG-DDFAGVPIQNRRLL--------GVEEREE 273
Query: 271 DFPRWVSRKDRQLLASPISSIQADIVVAKDGTGNYKTVAEAIEAAPSSSGRRTIIYIKAG 330
FPRW+ K+R++L P+S IQADI+V+KDG G KT++EAI+ AP +S RR IIY+KAG
Sbjct: 274 KFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAG 333
Query: 331 RYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIYDKVTTFRTATFAASGTGVILRDMTF 390
RYEE NLKVGRKK NLMFVGDGKG TVISGGKSI+D +TTF TA+FAA+G G I RD+TF
Sbjct: 334 RYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITF 393
Query: 391 ENSAGPSKHQAVALRLSADHAVVFRCSVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA 450
EN AGP+KHQAVALR+ ADHAV++RC++IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA
Sbjct: 394 ENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA 453
Query: 451 VVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACRILATSDLEASKGSFPTFL 510
VV Q+C+IYARKPM QKNTITAQ+RKDPNQNTGISIHA R+LA SDL+A+ GS T+L
Sbjct: 454 AVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYL 513
Query: 511 GRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPG 570
GRPWKL+SR VYM+S +G H+H RGWLEW +FALDTLYYGEY+NSGPG+ +GQRV WPG
Sbjct: 514 GRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPG 573
Query: 571 YRVITSTIEASKFTVGQFIYGSAWLPSTGVSFSAGLS 604
YRVI ST EA++FTV +FIYGS+WLPSTGVSF AGLS
Sbjct: 574 YRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597
BLAST of Sgr021601 vs. ExPASy Swiss-Prot
Match:
P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)
HSP 1 Score: 447.6 bits (1150), Expect = 2.3e-124
Identity = 260/579 (44.91%), Postives = 356/579 (61.49%), Query Frame = 0
Query: 36 RRSTRVVLLSFLSLLLIAA-SAVSAVLLLRHRISDFGRGGHAFPLKPTQAISRACSRTLY 95
++ ++ L F +LL++AA + A + R D G H LK +CS T Y
Sbjct: 25 KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILK------SSCSSTRY 84
Query: 96 PALCVSSLLDFPGALS--ADVHDLVHISLNVTLQRLTKALYSSSQISASQISDPLALSAY 155
P LC S++ P A D++ +SLN+T + + ++ A
Sbjct: 85 PDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVAL 144
Query: 156 EDCMELLDDAVDAFSRSL--LSIVPS----SDTNHDVLTWLSAALTYQDTCTEGLA--DV 215
DC+E +D+ +D +++ L P+ S D+ T +SAA+T Q TC +G + D
Sbjct: 145 HDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDA 204
Query: 216 SGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTD-DFSGVPIQNKRRLMQSQWPPENAAA 275
+ V+D ++ + ++ SN+LA+ TD D + N R+L++ E +
Sbjct: 205 NKHVRDALSDGQVHVEKMCSNALAMIKNM---TDTDMMIMRTSNNRKLIE-----ETSTV 264
Query: 276 EGEDFPRWVSRKDRQLLASPISSIQADIVVAKDGTGNYKTVAEAIEAAPSSSGRRTIIYI 335
+G +P W+S DR+LL S SS+ ++VVA DG+GN+KTVA ++ AAP +R II I
Sbjct: 265 DG--WPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRI 324
Query: 336 KAGRYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIYDKVTTFRTATFAASGTGVILRD 395
KAG Y EN++V +K N+MF+GDG+ T+I+G +++ D TTF++AT A G G + RD
Sbjct: 325 KAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARD 384
Query: 396 MTFENSAGPSKHQAVALRLSADHAVVFRCSVIGYQDTLYVHSNRQFFRECDIYGTVDFIF 455
+TF+N+AGPSKHQAVALR+ AD + + C ++ YQDTLYVHSNRQFF C I GTVDFIF
Sbjct: 385 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 444
Query: 456 GNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACRILATSDLEASKGSFP 515
GNA V Q+C+I+ARKP QKN +TAQ R DPNQNTGI I RI ATSDL+ +GSFP
Sbjct: 445 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFP 504
Query: 516 TFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVK 575
T+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ N+G GA RVK
Sbjct: 505 TYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVK 564
Query: 576 WPGYRVITSTIEASKFTVGQFIYGSAWLPSTGVSFSAGL 603
W G+RVITS EA FT G FI GS+WL STG FS GL
Sbjct: 565 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
BLAST of Sgr021601 vs. ExPASy Swiss-Prot
Match:
O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)
HSP 1 Score: 446.8 bits (1148), Expect = 3.9e-124
Identity = 262/579 (45.25%), Postives = 354/579 (61.14%), Query Frame = 0
Query: 36 RRSTRVVLLSFLSLLLIAA-SAVSAVLLLRHRISDFGRGGHAFPLKPTQAISRACSRTLY 95
++ ++ L F +LL++AA + A + R D G H LK +CS T Y
Sbjct: 25 KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILK------SSCSSTRY 84
Query: 96 PALCVSSLLDFPGALS--ADVHDLVHISLNVTLQRLTKALYSSSQISASQISDPLALSAY 155
P LC S++ P A D++ +SLN+T + + ++ A
Sbjct: 85 PDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVAL 144
Query: 156 EDCMELLDDAVDAFSRSL--LSIVPS----SDTNHDVLTWLSAALTYQDTCTEGLA--DV 215
DC+E +D+ +D +++ L P+ S D+ T +SAA+T Q TC +G + D
Sbjct: 145 HDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDA 204
Query: 216 SGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTD-DFSGVPIQNKRRLMQSQWPPENAAA 275
+ V+D ++ + ++ SN+LA+ TD D + N R+L + E +
Sbjct: 205 NKHVRDALSDGQVHVEKMCSNALAMIKNM---TDTDMMIMRTSNNRKLTE-----ETSTV 264
Query: 276 EGEDFPRWVSRKDRQLLASPISSIQADIVVAKDGTGNYKTVAEAIEAAPSSSGRRTIIYI 335
+G +P W+S DR+LL S SS+ + VVA DG+GN+KTVA A+ AAP +R II I
Sbjct: 265 DG--WPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRI 324
Query: 336 KAGRYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIYDKVTTFRTATFAASGTGVILRD 395
KAG Y EN++V +K N+MF+GDG+ T+I+G +++ D TTF++AT A G G + RD
Sbjct: 325 KAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARD 384
Query: 396 MTFENSAGPSKHQAVALRLSADHAVVFRCSVIGYQDTLYVHSNRQFFRECDIYGTVDFIF 455
+TF+N+AGPSKHQAVALR+ AD + + C ++ YQDTLYVHSNRQFF C I GTVDFIF
Sbjct: 385 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 444
Query: 456 GNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACRILATSDLEASKGSFP 515
GNA V Q+C+I+ARKP QKN +TAQ R DPNQNTGI I RI ATSDL+ +GSFP
Sbjct: 445 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFP 504
Query: 516 TFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVK 575
T+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ NSG GA RVK
Sbjct: 505 TYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVK 564
Query: 576 WPGYRVITSTIEASKFTVGQFIYGSAWLPSTGVSFSAGL 603
W G+RVITS EA FT G FI GS+WL STG FS GL
Sbjct: 565 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
BLAST of Sgr021601 vs. ExPASy Swiss-Prot
Match:
O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)
HSP 1 Score: 441.4 bits (1134), Expect = 1.6e-122
Identity = 254/589 (43.12%), Postives = 358/589 (60.78%), Query Frame = 0
Query: 36 RRSTRVVLLS-FLSLLLIAASA-VSAVLLLRHRISDFGRGGHAFPLKPTQAISRACSRTL 95
+++ ++VLLS ++LL +AA A +SA + HA + +CS T
Sbjct: 15 KKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHA-------VLRSSCSSTR 74
Query: 96 YPALCVSSLLDFPGALSADVHDLVHISLNVTLQRLTKALYSSSQ-ISASQISDPLALSAY 155
YP LC+S+++ G D++ S+N+T+ + ++ + I + P +A
Sbjct: 75 YPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTAL 134
Query: 156 EDCMELLDDAVDAFSRSL--LSIVPSSDT--NH--DVLTWLSAALTYQDTCTEGLA--DV 215
DC+E +D+ +D ++ L + P+ T H D+ T +S+A+T Q+TC +G + D
Sbjct: 135 HDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDA 194
Query: 216 SGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTD----DFSGVPIQNKRRLMQSQWPPEN 275
V+ + + + SN+LA+ TD + N R+L +
Sbjct: 195 DKQVRKALLKGQIHVEHMCSNALAMIKNM-TDTDIANFEQKAKITSNNRKLKEENQETTV 254
Query: 276 AA-------AEGEDFPRWVSRKDRQLLASPISSIQADIVVAKDGTGNYKTVAEAIEAAPS 335
A + E +P W+S DR+LL S ++AD VA DG+G +KTVA A+ AAP
Sbjct: 255 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 314
Query: 336 SSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIYDKVTTFRTATFA 395
+S +R +I+IKAG Y EN++V +KK N+MF+GDG+ T+I+G +++ D TTF +AT A
Sbjct: 315 NSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 374
Query: 396 ASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIGYQDTLYVHSNRQFFREC 455
A G + RD+TF+N+AGPSKHQAVALR+ +D + + C ++ YQDTLYVHSNRQFF +C
Sbjct: 375 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 434
Query: 456 DIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACRILATS 515
I GTVDFIFGNA VV Q C+I+AR+P QKN +TAQ R DPNQNTGI I CRI ATS
Sbjct: 435 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 494
Query: 516 DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 575
DL++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 495 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 554
Query: 576 PGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGVSFSAGL 603
GA RVKW G++VIT+ EA K+T GQFI G WL STG FS GL
Sbjct: 555 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
BLAST of Sgr021601 vs. ExPASy TrEMBL
Match:
A0A6J1DY95 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111024627 PE=3 SV=1)
HSP 1 Score: 993.4 bits (2567), Expect = 4.1e-286
Identity = 505/606 (83.33%), Postives = 545/606 (89.93%), Query Frame = 0
Query: 1 MNYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAV 60
MNYGKLHS++SGD TP NF LS+ S+ P+ R TRVVLLS LS+LLI ASA+SAV
Sbjct: 1 MNYGKLHSTSSGD-TPANFELSNSNSSI--SSPNRRTRTRVVLLSLLSILLIMASAISAV 60
Query: 61 LLLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHIS 120
+ +R I G G KP+QAISRACSRTLYPA+CVSSL++FPGAL+ADVHDLVHIS
Sbjct: 61 VFVRSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHIS 120
Query: 121 LNVTLQRLTKALYSSSQISASQISDPLALSAYEDCMELLDDAVDAFSRSLLSIVPSSDTN 180
LNVTLQRLTKALYSSSQI SQISDPL LSA+ DC+ELLDDAVDAFSRSL S S N
Sbjct: 121 LNVTLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAAS---N 180
Query: 181 HDVLTWLSAALTYQDTCTEGLADVS-GPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDD 240
HDVLTWLSAALTY DTCTEGLADVS G VKDQMT L DLSQLISNSLAIFSGFGGGT +
Sbjct: 181 HDVLTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGE 240
Query: 241 FSGVPIQNKRRLMQSQWPPENAAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAKDGTG 300
FSGVP+QN+RRLM+++WP EN GE+FP WVSRKDRQLL SPIS+IQADIVVAKDGTG
Sbjct: 241 FSGVPVQNRRRLMEAEWPQENG---GEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 300
Query: 301 NYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 360
YKTVAEAI AAPSSSGRRT+IY+KAG+Y EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 301 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 360
Query: 361 IYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIGYQD 420
++DKVTTFRTATFAASGTG+ILRDMTFEN+AGP +HQAVALR+SADHAVVFRC++IGYQD
Sbjct: 361 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 420
Query: 421 TLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQN 480
TLYVHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQDRKDPNQN
Sbjct: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 480
Query: 481 TGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 540
TGISIHAC+ILA SDLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 481 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 540
Query: 541 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGVSFS 600
ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITST+EA KFTVGQFIYGSAWLPSTGVSFS
Sbjct: 541 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFS 597
Query: 601 AGLSAN 606
AGLSA+
Sbjct: 601 AGLSAS 597
BLAST of Sgr021601 vs. ExPASy TrEMBL
Match:
A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)
HSP 1 Score: 929.1 bits (2400), Expect = 9.4e-267
Identity = 480/609 (78.82%), Postives = 532/609 (87.36%), Query Frame = 0
Query: 2 NYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAVL 61
NY KLHSS SGD TP F +SH P PP +SRR TR++ +S +S+LLI ASAVSA +
Sbjct: 3 NYNKLHSSASGD-TPATFQVSHTP--NPPSSSASRRRTRILFVSLISILLITASAVSAAV 62
Query: 62 LLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHISL 121
LL + S F KPTQAIS ACS TLYP+LC SSLL FPG+LSADVH LVHISL
Sbjct: 63 LLHSQSSHFPHN------KPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISL 122
Query: 122 NVTLQRLTKALYSSSQISASQIS-DPLALSAYEDCMELLDDAVDAFSRSLLSIVPSSDTN 181
N+TLQ LTKALY+SSQI QIS +PLA SAY+DC+ELL+DAVDAFS SL S +S +N
Sbjct: 123 NLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFS---TSASN 182
Query: 182 HDVLTWLSAALTYQDTCTEGLADVSGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDDF 241
HD++TWLSAALTY DTC++G ADV PVKDQM L+DLS++ISNSLAIFSG+G
Sbjct: 183 HDIMTWLSAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYG------ 242
Query: 242 SGVPIQNKRRLMQS--QWPPEN--AAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAKD 301
G+P+QN+RRLM+S +W EN A AEGE FP WVS KDR+LLA+PIS+IQADIVVAKD
Sbjct: 243 EGLPVQNRRRLMESDREWAAENGVAVAEGE-FPGWVSGKDRRLLAAPISAIQADIVVAKD 302
Query: 302 GTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISG 361
G+G +KTVAEAIEAAPSSSGRR +IYIKAG+YEEENLKVGRKKTNLMFVGDGKGITVISG
Sbjct: 303 GSGKFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISG 362
Query: 362 GKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIG 421
GKS++DKVTTFRTATFA SGTG+ILRDMTFEN+AGPSKHQAVALR+SADHAVV+RC++IG
Sbjct: 363 GKSVFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIG 422
Query: 422 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDP 481
YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+RKDP
Sbjct: 423 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDP 482
Query: 482 NQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ 541
NQNTGISIHAC+ILAT+DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ
Sbjct: 483 NQNTGISIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ 542
Query: 542 GSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGV 601
GSFALDTLYYGEYMNSGPGAA GQRV WPGYRVITSTIEA+KFTVGQFIYGS+WLPSTGV
Sbjct: 543 GSFALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGV 592
Query: 602 SFSAGLSAN 606
SFSAGLSAN
Sbjct: 603 SFSAGLSAN 592
BLAST of Sgr021601 vs. ExPASy TrEMBL
Match:
A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)
HSP 1 Score: 923.3 bits (2385), Expect = 5.2e-265
Identity = 475/609 (78.00%), Postives = 529/609 (86.86%), Query Frame = 0
Query: 2 NYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAVL 61
NY KLHSS SGD T+F +S++ + PPPPP S R++L+SF S+LLI AS VSAV+
Sbjct: 4 NYNKLHSSRSGDTPVTSFRVSNDHSNPPPPPPPSSSHRRILLISFFSILLITASTVSAVV 63
Query: 62 LLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHISL 121
L+ RI+ H KPTQAISRACS TLYP+LC+SSLL PG+LSADVHDLVHISL
Sbjct: 64 LVHSRITT-STESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDLVHISL 123
Query: 122 NVTLQRLTKALYSSSQISASQIS-DPLALSAYEDCMELLDDAVDAFSRSLLSIVPSSDTN 181
N+TLQ LTKALYS+SQI SQIS DPLA SAYEDCMELL+DAV+AFS SL S ++ TN
Sbjct: 124 NLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFS---TTATN 183
Query: 182 HDVLTWLSAALTYQDTCTEGLADVSGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDDF 241
HDV+TWLSAALTY DTCT+G +DV+ PVKDQ+T L+DLS++ISNSLAIFSGFGGG
Sbjct: 184 HDVMTWLSAALTYHDTCTDGFSDVADPVKDQITAKLSDLSEMISNSLAIFSGFGGG---- 243
Query: 242 SGVPIQN--KRRLMQS--QWPPENAAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAKD 301
+P++N +RRLM+S QW E +G DFPRWVS KDR+LLA+PISSIQADIVVAKD
Sbjct: 244 -DLPVENRRRRRLMESDEQWLAEENNGDG-DFPRWVSGKDRRLLAAPISSIQADIVVAKD 303
Query: 302 GTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISG 361
G+G +KTVAEAI AAPSSS RR +IYIKAG+YEEENLKVGRKKTNLMFVGDGKGITVISG
Sbjct: 304 GSGKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISG 363
Query: 362 GKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIG 421
GKS+YDKVTTFRTATFA SGTG+ILRDMTFEN+AGP+KHQAVALRLSADH VVFR +VIG
Sbjct: 364 GKSVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIG 423
Query: 422 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDP 481
YQDTLYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM QKNTITAQDRKDP
Sbjct: 424 YQDTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDP 483
Query: 482 NQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQ 541
NQNTGISIHAC+I+AT DL+ASKGSFPT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQ
Sbjct: 484 NQNTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQ 543
Query: 542 GSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGV 601
GSFALDTLYYGEYMNSGP AAVGQRVKWPGYRVITS IEA+KFTVGQFIYGS+WLPSTGV
Sbjct: 544 GSFALDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGV 602
Query: 602 SFSAGLSAN 606
SFSAGLS N
Sbjct: 604 SFSAGLSTN 602
BLAST of Sgr021601 vs. ExPASy TrEMBL
Match:
A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)
HSP 1 Score: 921.4 bits (2380), Expect = 2.0e-264
Identity = 481/610 (78.85%), Postives = 535/610 (87.70%), Query Frame = 0
Query: 2 NYGKLHSSTSGDNTPTNFHLSHEPIS-LPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAV 61
NY LHSS SGD ++F +S++ LPPP PSS R R++L+SF S+LLI ASAVSAV
Sbjct: 4 NYNMLHSSHSGDTPASSFRVSNDHSDPLPPPSPSSSR-RRILLISFFSILLITASAVSAV 63
Query: 62 LLLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHIS 121
+L+ RI+ H KPTQAISRACS TLYP+LC+SSLL PG+LSADVHDLVHIS
Sbjct: 64 VLVHSRIA-ASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHIS 123
Query: 122 LNVTLQRLTKALYSSSQISASQIS-DPLALSAYEDCMELLDDAVDAFSRSLLSIVPSSDT 181
LN+TLQ LTKALYS+SQI SQIS DPLA SAYEDCMELL+DAV+AFS SL S ++ T
Sbjct: 124 LNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFS---TTAT 183
Query: 182 NHDVLTWLSAALTYQDTCTEGLADVSGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDD 241
NHDV+TWLSAALTY DTCT+G +DV+ P+KDQ+T L+DLS++ISNSLAIFSGFGG DD
Sbjct: 184 NHDVMTWLSAALTYHDTCTDGFSDVADPIKDQITAKLSDLSEMISNSLAIFSGFGG--DD 243
Query: 242 FSGVPIQN--KRRLMQS--QWPPENAAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAK 301
+P++N +RRLM+S QW E +G DFPRWVS KDR+LLA+PISSIQADIVVAK
Sbjct: 244 ---LPVENRRRRRLMESDEQWLAEENNGDG-DFPRWVSGKDRRLLAAPISSIQADIVVAK 303
Query: 302 DGTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVIS 361
DG+G +KTVAEAI AAPSSSGRR +IYIKAG+YEEENLKVGRKKTNLMFVGDGKGITVIS
Sbjct: 304 DGSGKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVIS 363
Query: 362 GGKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVI 421
GGKS+YDKVTTFRTATFA SGTG+ILRDMTFEN+AGP+KHQAVALRLSADHAVVFR +VI
Sbjct: 364 GGKSVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVI 423
Query: 422 GYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKD 481
GYQDTLYVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM QKNTITAQDRKD
Sbjct: 424 GYQDTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKD 483
Query: 482 PNQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEW 541
PNQNTGISIHAC+I+AT DL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEW
Sbjct: 484 PNQNTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEW 543
Query: 542 QGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTG 601
QGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITS IEA+KFTVGQFIYGS+WLPSTG
Sbjct: 544 QGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTG 602
Query: 602 VSFSAGLSAN 606
VSFSAGLS N
Sbjct: 604 VSFSAGLSTN 602
BLAST of Sgr021601 vs. ExPASy TrEMBL
Match:
A0A6J1K6C1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491552 PE=3 SV=1)
HSP 1 Score: 919.1 bits (2374), Expect = 9.8e-264
Identity = 479/612 (78.27%), Postives = 531/612 (86.76%), Query Frame = 0
Query: 2 NYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPS---SRRSTRVVLLSFLSLLLIAASAVS 61
NY KLHSS SGD TP F +SH P PP PPS SRR TR++ +S +S+LLI ASAVS
Sbjct: 3 NYNKLHSSGSGD-TPATFQVSHTP--NPPHPPSSSASRRRTRILFVSLISILLITASAVS 62
Query: 62 AVLLLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVH 121
A +L+ S H KPTQAIS ACSRTLYP+LC SSLL FPG+LSADVH LVH
Sbjct: 63 AAVLIHSHSS------HIPHNKPTQAISHACSRTLYPSLCRSSLLSFPGSLSADVHHLVH 122
Query: 122 ISLNVTLQRLTKALYSSSQISASQIS-DPLALSAYEDCMELLDDAVDAFSRSLLSIVPSS 181
ISLN+TLQ LTKALY+SSQI QIS +PLA SAY+DC+ELL+DAVDAFS SL S +S
Sbjct: 123 ISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFS---TS 182
Query: 182 DTNHDVLTWLSAALTYQDTCTEGLADVSGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGT 241
+NHD++TWLSAALTY DTC++G ADV PVKDQM + L+DLS++ISNSLAIFSG+G
Sbjct: 183 ASNHDIMTWLSAALTYHDTCSDGFADVDDPVKDQMASRLSDLSEMISNSLAIFSGYG--- 242
Query: 242 DDFSGVPIQNKRRLMQS--QWPPEN--AAAEGEDFPRWVSRKDRQLLASPISSIQADIVV 301
G+P+QN+RRLM+S +W EN A AEGE FP WVS KDR+LLA+ IS+IQADIVV
Sbjct: 243 ---EGLPVQNRRRLMESDREWAAENGVAVAEGE-FPGWVSGKDRRLLAASISAIQADIVV 302
Query: 302 AKDGTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITV 361
AKDG+G +KTVAEAIEAAPSSSGRR +IYIKAG+YEEENLKVGRKKTNLMFVGDGKGITV
Sbjct: 303 AKDGSGKFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITV 362
Query: 362 ISGGKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCS 421
ISGGKS++DKVTTFRTATFA SGTG+ILRDMT EN+AGPSKHQAVALR+SADHAVV+RC+
Sbjct: 363 ISGGKSVFDKVTTFRTATFAGSGTGIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCN 422
Query: 422 VIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDR 481
+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+R
Sbjct: 423 IIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNR 482
Query: 482 KDPNQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWL 541
KDPNQNTGISIHAC+ILAT+DLEASKGSF TFLGRPWKLYSRVVYMVSSMGDHIHPRGWL
Sbjct: 483 KDPNQNTGISIHACKILATTDLEASKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWL 542
Query: 542 EWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPS 601
EWQGSFALDTLYYGEYMNSGPGAA GQRV WPGYRVITSTIEA+KFTVGQFIYGS+WLPS
Sbjct: 543 EWQGSFALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPS 595
Query: 602 TGVSFSAGLSAN 606
TGVSFSAGLS N
Sbjct: 603 TGVSFSAGLSGN 595
BLAST of Sgr021601 vs. TAIR 10
Match:
AT5G53370.1 (pectin methylesterase PCR fragment F )
HSP 1 Score: 738.4 bits (1905), Expect = 4.6e-213
Identity = 377/608 (62.01%), Postives = 466/608 (76.64%), Query Frame = 0
Query: 1 MNYGKLHSSTSGDNTPTNFHLSHEPISLPPPPPSSRRSTRVVLLSFLSLLLIAASAVSAV 60
M Y +L G + P+N + SL P ++ T+ L+ F +L ++ V
Sbjct: 1 MGYDRL-----GPSGPSNPNQKDPATSL----PELQKKTKTKLILF-TLAVLVVGVVCFG 60
Query: 61 LLLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPALCVSSLLDFPGALSADVHDLVHIS 120
+ R D G+ KPTQAISR CS++LYP LC+ +LLDFPG+L+AD ++L+HIS
Sbjct: 61 IFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHIS 120
Query: 121 LNVTLQRLTKALYSSSQISASQISDPLALSAYEDCMELLDDAVDAFSRSLLS-IVPSSDT 180
N TLQ+ +KALY+SS I+ +Q+ P SAY+ C+ELLDD+VDA +R+L S +V S D
Sbjct: 121 FNATLQKFSKALYTSSTITYTQM-PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE 180
Query: 181 NH-DVLTWLSAALTYQDTCTEGLADV---SGPVKDQMTTTLNDLSQLISNSLAIFSGFGG 240
+H DV+TWLS+A+T DTCT+G ++ G VKDQ+ + DLS+++SN LAIF+ G
Sbjct: 181 SHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFA---G 240
Query: 241 GTDDFSGVPIQNKRRLMQSQWPPENAAAEGEDFPRWVSRKDRQLLASPISSIQADIVVAK 300
D SGVP+ N R+L+ ++ E E+ P W+ R+DR+LL +P S+IQADI V+K
Sbjct: 241 KVKDLSGVPVVNNRKLLGTE--------ETEELPNWLKREDRELLGTPTSAIQADITVSK 300
Query: 301 DGTGNYKTVAEAIEAAPSSSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVIS 360
DG+G +KT+AEAI+ AP S RR +IY+KAGRYEEENLKVGRKKTNLMF+GDGKG TVI+
Sbjct: 301 DGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVIT 360
Query: 361 GGKSIYDKVTTFRTATFAASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVI 420
GGKSI D +TTF TATFAA+G G I+RDMTFEN AGP+KHQAVALR+ DHAVV+RC++I
Sbjct: 361 GGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNII 420
Query: 421 GYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKD 480
GYQD LYVHSNRQFFREC+IYGTVDFIFGNA V+ QSCNIYARKPM QQK TITAQ+RKD
Sbjct: 421 GYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKD 480
Query: 481 PNQNTGISIHACRILATSDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEW 540
PNQNTGISIHAC++LAT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW
Sbjct: 481 PNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEW 540
Query: 541 QGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTG 600
G FALD+LYYGEYMN G G+ +GQRVKWPGY VITST+EASKFTV QFI GS+WLPSTG
Sbjct: 541 NGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTG 586
Query: 601 VSFSAGLS 604
VSF +GLS
Sbjct: 601 VSFFSGLS 586
BLAST of Sgr021601 vs. TAIR 10
Match:
AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )
HSP 1 Score: 709.9 bits (1831), Expect = 1.7e-204
Identity = 365/577 (63.26%), Postives = 446/577 (77.30%), Query Frame = 0
Query: 31 PPPSSRRSTRVVLLS--FLSLLLIAASAVSAVLLLRHRISDFGRGGHAFPLKPTQAISRA 90
P ++RRS + +++S L++ LI A+A+ A + R +++ G KP+QAIS+A
Sbjct: 34 PENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPG---LARKPSQAISKA 93
Query: 91 CSRTLYPALCVSSLLDFPGALSA-DVHDLVHISLNVTLQRLTKALYSSSQISASQISDPL 150
C T +P LCV SL+DFPG+L+A DL+H+++N+TL + ALYSS+ +S + P
Sbjct: 94 CELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDM-PPR 153
Query: 151 ALSAYEDCMELLDDAVDAFSRSLLSIVPSSDTNHDVLTWLSAALTYQDTCTEGLADV-SG 210
A SAY+ C+ELLDD+VDA SR+L S+V SS DV TWLSAALT DTCTEG V G
Sbjct: 154 ARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTNHDTCTEGFDGVDDG 213
Query: 211 PVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDDFSGVPIQNKRRLMQSQWPPENAAAEGE 270
VKD MT L +LS+L+SN LAIFS G DDF+GVPIQN+R L E
Sbjct: 214 GVKDHMTAALQNLSELVSNCLAIFSASHDG-DDFAGVPIQNRRLL--------GVEEREE 273
Query: 271 DFPRWVSRKDRQLLASPISSIQADIVVAKDGTGNYKTVAEAIEAAPSSSGRRTIIYIKAG 330
FPRW+ K+R++L P+S IQADI+V+KDG G KT++EAI+ AP +S RR IIY+KAG
Sbjct: 274 KFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAG 333
Query: 331 RYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIYDKVTTFRTATFAASGTGVILRDMTF 390
RYEE NLKVGRKK NLMFVGDGKG TVISGGKSI+D +TTF TA+FAA+G G I RD+TF
Sbjct: 334 RYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITF 393
Query: 391 ENSAGPSKHQAVALRLSADHAVVFRCSVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA 450
EN AGP+KHQAVALR+ ADHAV++RC++IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA
Sbjct: 394 ENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA 453
Query: 451 VVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACRILATSDLEASKGSFPTFL 510
VV Q+C+IYARKPM QKNTITAQ+RKDPNQNTGISIHA R+LA SDL+A+ GS T+L
Sbjct: 454 AVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYL 513
Query: 511 GRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPG 570
GRPWKL+SR VYM+S +G H+H RGWLEW +FALDTLYYGEY+NSGPG+ +GQRV WPG
Sbjct: 514 GRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPG 573
Query: 571 YRVITSTIEASKFTVGQFIYGSAWLPSTGVSFSAGLS 604
YRVI ST EA++FTV +FIYGS+WLPSTGVSF AGLS
Sbjct: 574 YRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597
BLAST of Sgr021601 vs. TAIR 10
Match:
AT3G14310.1 (pectin methylesterase 3 )
HSP 1 Score: 441.4 bits (1134), Expect = 1.1e-123
Identity = 254/589 (43.12%), Postives = 358/589 (60.78%), Query Frame = 0
Query: 36 RRSTRVVLLS-FLSLLLIAASA-VSAVLLLRHRISDFGRGGHAFPLKPTQAISRACSRTL 95
+++ ++VLLS ++LL +AA A +SA + HA + +CS T
Sbjct: 15 KKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHA-------VLRSSCSSTR 74
Query: 96 YPALCVSSLLDFPGALSADVHDLVHISLNVTLQRLTKALYSSSQ-ISASQISDPLALSAY 155
YP LC+S+++ G D++ S+N+T+ + ++ + I + P +A
Sbjct: 75 YPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTAL 134
Query: 156 EDCMELLDDAVDAFSRSL--LSIVPSSDT--NH--DVLTWLSAALTYQDTCTEGLA--DV 215
DC+E +D+ +D ++ L + P+ T H D+ T +S+A+T Q+TC +G + D
Sbjct: 135 HDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDA 194
Query: 216 SGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTD----DFSGVPIQNKRRLMQSQWPPEN 275
V+ + + + SN+LA+ TD + N R+L +
Sbjct: 195 DKQVRKALLKGQIHVEHMCSNALAMIKNM-TDTDIANFEQKAKITSNNRKLKEENQETTV 254
Query: 276 AA-------AEGEDFPRWVSRKDRQLLASPISSIQADIVVAKDGTGNYKTVAEAIEAAPS 335
A + E +P W+S DR+LL S ++AD VA DG+G +KTVA A+ AAP
Sbjct: 255 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 314
Query: 336 SSGRRTIIYIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIYDKVTTFRTATFA 395
+S +R +I+IKAG Y EN++V +KK N+MF+GDG+ T+I+G +++ D TTF +AT A
Sbjct: 315 NSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 374
Query: 396 ASGTGVILRDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIGYQDTLYVHSNRQFFREC 455
A G + RD+TF+N+AGPSKHQAVALR+ +D + + C ++ YQDTLYVHSNRQFF +C
Sbjct: 375 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 434
Query: 456 DIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACRILATS 515
I GTVDFIFGNA VV Q C+I+AR+P QKN +TAQ R DPNQNTGI I CRI ATS
Sbjct: 435 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 494
Query: 516 DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 575
DL++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 495 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 554
Query: 576 PGAAVGQRVKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGVSFSAGL 603
GA RVKW G++VIT+ EA K+T GQFI G WL STG FS GL
Sbjct: 555 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
BLAST of Sgr021601 vs. TAIR 10
Match:
AT1G53830.1 (pectin methylesterase 2 )
HSP 1 Score: 436.8 bits (1122), Expect = 2.8e-122
Identity = 252/581 (43.37%), Postives = 342/581 (58.86%), Query Frame = 0
Query: 38 STRVVLLSFLSLLLIAASAVSAVLLLRHRISDFGRGGHAFPLKPTQAISRACSRTLYPAL 97
S + LL S++ IAA+ + +I+ HA + CS TLYP L
Sbjct: 24 SAAIALLLLASIVGIAATTTNQ--NKNQKITTLSSTSHAI-------LKSVCSSTLYPEL 83
Query: 98 CVSSLLDFPGALSADVHDLVHISLNVTLQRLTKALYSSSQISASQIS-DPLALSAYEDCM 157
C S++ G +++ SLN+T + + ++ ++ A + P ++A DC+
Sbjct: 84 CFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCL 143
Query: 158 ELLDDAVDAFSRSL--LSIVPSSDT----NHDVLTWLSAALTYQDTCTEGLA--DVSGPV 217
E +D+ +D ++ L P + D+ T +S+A+T Q TC +G + D V
Sbjct: 144 ETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKV 203
Query: 218 KDQMTTTLNDLSQLISNSLAIFSGFGGG-------TDDFSGVPIQNKRRLMQSQWPPENA 277
+ + + + SN+LA+ D S N R+L +
Sbjct: 204 RKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKE-----VTG 263
Query: 278 AAEGEDFPRWVSRKDRQLLASPISSIQADIVVAKDGTGNYKTVAEAIEAAPSSSGRRTII 337
+ + +P+W+S DR+LL S+I+AD VA DG+G++ TVA A+ AAP S +R +I
Sbjct: 264 DLDSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVI 323
Query: 338 YIKAGRYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIYDKVTTFRTATFAASGTGVIL 397
+IKAG Y EN++V +KKTN+MF+GDG+G T+I+G +++ D TTF +AT AA G +
Sbjct: 324 HIKAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLA 383
Query: 398 RDMTFENSAGPSKHQAVALRLSADHAVVFRCSVIGYQDTLYVHSNRQFFRECDIYGTVDF 457
RD+TF+N+AGPSKHQAVALR+ +D + ++C + YQDTLYVHSNRQFF +C I GTVDF
Sbjct: 384 RDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDF 443
Query: 458 IFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACRILATSDLEASKGS 517
IFGNA V Q C+I AR+P QKN +TAQ R DPNQNTGI I CRI TSDL A KG+
Sbjct: 444 IFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGT 503
Query: 518 FPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQR 577
FPT+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA R
Sbjct: 504 FPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANR 563
Query: 578 VKWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGVSFSAGL 603
VKW GY+VITS EA FT GQFI G WL STG FS L
Sbjct: 564 VKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
BLAST of Sgr021601 vs. TAIR 10
Match:
AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )
HSP 1 Score: 431.0 bits (1107), Expect = 1.6e-120
Identity = 226/460 (49.13%), Postives = 303/460 (65.87%), Query Frame = 0
Query: 150 SAYEDCMELLDDAVDAFSRSLLSIV-------PSSDTNHDVLTWLSAALTYQDTCTEGLA 209
+A DC++LLD A + S + + + D D+ TW+SAAL+ QDTC +G
Sbjct: 79 NAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFE 138
Query: 210 DVSGPVKDQMTTTLNDLSQLISNSLAIFSGFGGGTDDFSGVPIQNKRRLMQSQWPPENAA 269
+G +K + L+ + + N L + P + K + +++Q A
Sbjct: 139 GTNGIIKKIVAGGLSKVGTTVRNLLTM----------VHSPPSKPKPKPIKAQ-TMTKAH 198
Query: 270 AEGEDFPRWVSRKDRQLLASPISSIQADIVVAKDGTGNYKTVAEAIEAAPSSSGRRTIIY 329
+ FP WV DR+LL + ++ AD VVA DGTGN+ T+++A+ AAP S +R +I+
Sbjct: 199 SGFSKFPSWVKPGDRKLLQTDNITV-ADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIH 258
Query: 330 IKAGRYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIYDKVTTFRTATFAASGTGVILR 389
+K G Y EN+++ +KK N+M VGDG TVI+G +S D TTFR+ATFA SG G I R
Sbjct: 259 VKRGVY-VENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIAR 318
Query: 390 DMTFENSAGPSKHQAVALRLSADHAVVFRCSVIGYQDTLYVHSNRQFFRECDIYGTVDFI 449
D+TF+N+AGP KHQAVA+R D V +RC++ GYQDTLY HS RQFFREC I GTVDFI
Sbjct: 319 DITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFI 378
Query: 450 FGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACRILATSDLEASKGSF 509
FG+A VFQSC I A++ +P QKN+ITAQ RKDPN+ TG +I I A +DL + +
Sbjct: 379 FGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT 438
Query: 510 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRV 569
T+LGRPWKLYSR V+M + M D I+P GWLEW G+FALDTLYYGEYMNSGPGA++ +RV
Sbjct: 439 ATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRV 498
Query: 570 KWPGYRVITSTIEASKFTVGQFIYGSAWLPSTGVSFSAGL 603
KWPGY V+ ++ EA+ FTV Q I G+ WLPSTG++F AGL
Sbjct: 499 KWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022158049.1 | 8.4e-286 | 83.33 | probable pectinesterase/pectinesterase inhibitor 61 [Momordica charantia] | [more] |
| XP_022957644.1 | 2.0e-266 | 78.82 | probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata] | [more] |
| XP_023533011.1 | 8.2e-265 | 78.33 | probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita pepo subsp. pepo] | [more] |
| XP_022972980.1 | 1.1e-264 | 78.00 | probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita maxima] | [more] |
| KAG6579321.1 | 2.4e-264 | 78.85 | putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9FK05 | 6.4e-212 | 62.01 | Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... | [more] |
| Q9M3B0 | 2.4e-203 | 63.26 | Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... | [more] |
| P83948 | 2.3e-124 | 44.91 | Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1 | [more] |
| O04886 | 3.9e-124 | 45.25 | Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1 | [more] |
| O49006 | 1.6e-122 | 43.12 | Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DY95 | 4.1e-286 | 83.33 | Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111024627 PE=3 SV=1 | [more] |
| A0A6J1H2K1 | 9.4e-267 | 78.82 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1 | [more] |
| A0A6J1IA52 | 5.2e-265 | 78.00 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1 | [more] |
| A0A6J1E2V1 | 2.0e-264 | 78.85 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1 | [more] |
| A0A6J1K6C1 | 9.8e-264 | 78.27 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491552 PE=3 SV=1 | [more] |
