Sgr021331 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr021331 vs. NCBI nr
Match: XP_023007070.1 (DNA polymerase alpha catalytic subunit [Cucurbita maxima])

HSP 1 Score: 2731.1 bits (7078), Expect = 0.0e+00
Identity = 1391/1548 (89.86%), Postives = 1458/1548 (94.19%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS ANRRRSRGSEA ARLQALERLKAIR+GGRRSEAGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSAANRRRSRGSEATARLQALERLKAIRTGGRRSEAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKA   SSDESDGE EKPKKRK+EKKE QP
Sbjct: 64   LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAGICSSDESDGEPEKPKKRKSEKKEAQP 123

Query: 880  KKPSS-SLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKPSS SLSAAAAMMGKQKLSSMFTSSIFRKT +DDKAKGLACDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSTSLSAAAAMMGKQKLSSMFTSSIFRKTGKDDKAKGLACDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA PIS+TFAP+PA+KCEG+ AQSLNL  GSEL+K T N NSGMT+   N 
Sbjct: 184  RERRRKGQIGATPISKTFAPVPAMKCEGVIAQSLNLTGGSELVKGTVNGNSGMTKDFTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LE VRA IE+ GNGETK+F+ K+DL+S+++L  + QSHN S+KEDVIEDNMP+VVETK+
Sbjct: 244  DLESVRADIEIQGNGETKKFDSKDDLDSEMNLVSVGQSHNPSIKEDVIEDNMPIVVETKS 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            E L+KKEPVCTLNA I++  KDPALSATAGWQAVRSEGSGN DSAA+ SE+KS FDID D
Sbjct: 304  EALVKKEPVCTLNATISDV-KDPALSATAGWQAVRSEGSGNADSAADTSEDKSHFDIDAD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFY+VDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKN+QRC+YAIPSA FLHSD
Sbjct: 364  GSLPFYMVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNVQRCVYAIPSAFFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EMLKLQ DAEQSQLS TDLRTKLQEVTAGLKNEIA+QLL LNV TFSMTPVKRKYAFER 
Sbjct: 424  EMLKLQNDAEQSQLSPTDLRTKLQEVTAGLKNEIAQQLLDLNVPTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIP GE+YVLKINYPFKHPPLP DLKGESFCALLGTHRSALELLLIKRKIKGPSWLSIS 
Sbjct: 484  DIPTGENYVLKINYPFKHPPLPADLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISK 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSSCPGSQRVSWCKFEVI+DS KDVQISTSSSKTLEIP MI TAINIKTIINE+QNVNEI
Sbjct: 544  FSSCPGSQRVSWCKFEVIIDSPKDVQISTSSSKTLEIPPMIATAINIKTIINEKQNVNEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRN KA SN
Sbjct: 604  VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNSKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPS MWSKIGRL
Sbjct: 664  VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSCMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQL+K
Sbjct: 724  KRSVMPKLGKGGGIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLSK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E  PHDIPRM+ ASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLWGR
Sbjct: 784  DRKEVTPHDIPRMYHASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWGR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDK S+YVKEKK+VKKR N G EEK++D  D+DDA
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKISTYVKEKKMVKKRTNHGSEEKNLDNVDLDDA 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            N+E APN  SGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NLE-APNTESGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGV+PRLPSSK+TGVLPELLKNLVQRRRMVKSWMKNASG+KLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVIPRLPSSKVTGVLPELLKNLVQRRRMVKSWMKNASGIKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            DIG+VKAIA KVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER
Sbjct: 1084 DIGQVKAIAVKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDV ESIHDSL+KIQEDMRKGQVALEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVTESIHDSLVKIQEDMRKGQVALEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGI 2019
            ITKTLTKPPEAYPDARNQPHVQVA RLKQMGYSTGCSVGDTIPYIICCEQGSTSGGS GI
Sbjct: 1204 ITKTLTKPPEAYPDARNQPHVQVALRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSVGI 1263

Query: 2020 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQ 2079
            AQRARHPDELKKEDGKWMIDI YYLSQQIHPVVSRLCASIQGTSPERLADCLG+DSSKFQ
Sbjct: 1264 AQRARHPDELKKEDGKWMIDIVYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFQ 1323

Query: 2080 IKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSE 2139
             KSSEVS SDVSSSLLCS+ND ERYQGC PLTLTCPSCSGTFECPAIFSSI KS +GK E
Sbjct: 1324 NKSSEVSRSDVSSSLLCSINDEERYQGCIPLTLTCPSCSGTFECPAIFSSIYKSADGKQE 1383

Query: 2140 RPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDET 2199
            +  VDEPT KFWN L CPKCPDEA+AGR+TPGMIANQVKRQAERFIS+YYNGL+MCEDET
Sbjct: 1384 K-AVDEPTSKFWNNLRCPKCPDEASAGRMTPGMIANQVKRQAERFISMYYNGLLMCEDET 1443

Query: 2200 CKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEK 2259
            CKYATRAVNLR+MGD+EKGTICPNY HCNGRLIRKYTE DLYKQLAY+S+ LDT+RCMEK
Sbjct: 1444 CKYATRAVNLRVMGDSEKGTICPNYTHCNGRLIRKYTEVDLYKQLAYFSHTLDTIRCMEK 1503

Query: 2260 LEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVTI 2305
            LEVHARVTLEKEMAKIRPIVELAASTIQS+RDRSAY WVQLQD  VT+
Sbjct: 1504 LEVHARVTLEKEMAKIRPIVELAASTIQSLRDRSAYGWVQLQDFVVTV 1548

BLAST of Sgr021331 vs. NCBI nr
Match: XP_023534068.1 (DNA polymerase alpha catalytic subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2728.7 bits (7072), Expect = 0.0e+00
Identity = 1390/1548 (89.79%), Postives = 1457/1548 (94.12%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS ANRRRSRGSEA ARLQALERLKAIR+GGRRSEAGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSAANRRRSRGSEATARLQALERLKAIRTGGRRSEAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKA    SDESDGE EKPKKRK+EKKE QP
Sbjct: 64   LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAGICFSDESDGEPEKPKKRKSEKKEAQP 123

Query: 880  KKPSS-SLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKPSS SLSAAAAMMGKQKLSSMFTSSIFRKT +DDKAKGLACDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSTSLSAAAAMMGKQKLSSMFTSSIFRKTGKDDKAKGLACDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA PIS+TFAP+P++KCEG+ AQSLNL  GSEL+K T N NSGMT+   N 
Sbjct: 184  RERRRKGQIGATPISKTFAPVPSMKCEGVIAQSLNLTGGSELVKGTVNGNSGMTKDFTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LE VRA IE+ GNGETK+F+ K+DL+S+I+L  + QSHN S+KEDVIEDNMP+VVETK+
Sbjct: 244  DLESVRADIEIQGNGETKKFDSKDDLDSEINLVSVGQSHNPSIKEDVIEDNMPIVVETKS 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            E L+KKEPVCTLNA I++  KDPALSATAGWQAVRSEGSGN DSAA+ SE+KS FDID D
Sbjct: 304  ESLVKKEPVCTLNATISDV-KDPALSATAGWQAVRSEGSGNADSAADTSEDKSHFDIDAD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFY+VDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKN+QRC+YAIPSASFLHSD
Sbjct: 364  GSLPFYMVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNVQRCVYAIPSASFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EMLKLQ DAEQSQLS TDLRTKLQEVTAGLKNEIA+QLL LNV TFSMTPVKRKYAFER 
Sbjct: 424  EMLKLQNDAEQSQLSPTDLRTKLQEVTAGLKNEIAQQLLDLNVPTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIP GE+YVLKINYPFKHPPLP DLKGESFCALLGTHRSALELLLIKRKIKGPSWLSIS 
Sbjct: 484  DIPTGENYVLKINYPFKHPPLPADLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISK 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSSC  SQRVSWCKFEVI+DS KDVQISTSSSKTLEIP MIVTAINIKTIINE+QNVNEI
Sbjct: 544  FSSCHVSQRVSWCKFEVIIDSPKDVQISTSSSKTLEIPPMIVTAINIKTIINEKQNVNEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRN KA SN
Sbjct: 604  VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNSKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPS MWSKIGRL
Sbjct: 664  VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSCMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK
Sbjct: 724  KRSVMPKLGKGGGIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E  PHDIPRM+ ASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLWGR
Sbjct: 784  DRKEVTPHDIPRMYHASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWGR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDK S+YVKEKK+VKKR N G EEK++D  D+DDA
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKISTYVKEKKMVKKRTNHGSEEKNLDNVDLDDA 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            N+E APN  SGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NIE-APNTESGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGV+P LPSSK+TGVLPELLKNLVQRRRMVKSWMKNASG+KLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVIPCLPSSKVTGVLPELLKNLVQRRRMVKSWMKNASGIKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            DIG+VKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDG PYEVIER
Sbjct: 1084 DIGQVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGTPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDV ESIHDSL+KIQEDMRKGQVALEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVTESIHDSLVKIQEDMRKGQVALEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGI 2019
            ITKTLTKPPEAYPDARNQPHVQVA RLKQMGYSTGCSVGDTIPYIICCEQGSTSGGS GI
Sbjct: 1204 ITKTLTKPPEAYPDARNQPHVQVALRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSVGI 1263

Query: 2020 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQ 2079
            AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG+DSSKFQ
Sbjct: 1264 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFQ 1323

Query: 2080 IKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSE 2139
             KSSEVS SDVSSSLLCS+ND ERYQGC PLTLTCPSCSGTFECPAIFSSI KS +GK E
Sbjct: 1324 NKSSEVSRSDVSSSLLCSINDEERYQGCIPLTLTCPSCSGTFECPAIFSSIYKSADGKQE 1383

Query: 2140 RPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDET 2199
            +  VDEPT KFWN L CPKCPDEA+AGR+TPGMI+NQVKRQAERFIS+YYNGL+MCEDET
Sbjct: 1384 K-AVDEPTSKFWNNLRCPKCPDEASAGRMTPGMISNQVKRQAERFISMYYNGLLMCEDET 1443

Query: 2200 CKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEK 2259
            CKYATRAVNLR+MGD+EKGTICPNY HCNGRLIRKYTE DLYKQLAY+S+ LDT+RCMEK
Sbjct: 1444 CKYATRAVNLRVMGDSEKGTICPNYTHCNGRLIRKYTEVDLYKQLAYFSHTLDTIRCMEK 1503

Query: 2260 LEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVTI 2305
            LEVHARVTLEKEMAKIRPIVELAASTIQS+RDRSAY WVQLQD  VT+
Sbjct: 1504 LEVHARVTLEKEMAKIRPIVELAASTIQSLRDRSAYGWVQLQDFVVTV 1548

BLAST of Sgr021331 vs. NCBI nr
Match: KAG6605204.1 (DNA polymerase alpha catalytic subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2726.0 bits (7065), Expect = 0.0e+00
Identity = 1387/1548 (89.60%), Postives = 1456/1548 (94.06%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS ANRRRSRGSEA ARLQALERLKAIR+GGRRSEAGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSAANRRRSRGSEATARLQALERLKAIRTGGRRSEAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKA    SDESDGE EKPKKRK+EKKE QP
Sbjct: 64   LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAGICFSDESDGEPEKPKKRKSEKKEAQP 123

Query: 880  KKPSS-SLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKPSS SLSAAAAMMGKQKLSSMFTSSIFRKT +DDKAKGLACDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSTSLSAAAAMMGKQKLSSMFTSSIFRKTGKDDKAKGLACDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA PIS+TFAP+ A+KCEG+ AQSLNL  GSEL+K T N NSGMT+   N 
Sbjct: 184  RERRRKGQIGATPISKTFAPVSAMKCEGVIAQSLNLTGGSELVKGTVNGNSGMTKDFTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LE VRA IE+ GNGETK+F+ K++L+S+++L  + QSHN S+K+DVIEDNMP VVETK+
Sbjct: 244  DLESVRADIEIQGNGETKKFDSKDNLDSEMNLVSVGQSHNPSIKDDVIEDNMPTVVETKS 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            E L+KKEPVCTLNA I++  KDPALSATAGWQAVRSEGSGN DSAA+ SE+KS FDID D
Sbjct: 304  EALVKKEPVCTLNATISDV-KDPALSATAGWQAVRSEGSGNADSAADTSEDKSHFDIDAD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFY+VDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKN+QRC+YAIPSASFLHSD
Sbjct: 364  GSLPFYMVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNVQRCVYAIPSASFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EMLKLQ DAEQSQLS TDLRTKLQEVTAGLKNEIA+QLL LNV TFSMTPVKRKYAFER 
Sbjct: 424  EMLKLQNDAEQSQLSPTDLRTKLQEVTAGLKNEIAQQLLDLNVPTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIP GE+YVLKINYPFKHPPLP DLKGESFCALLGTHRSALELLLIKRKIKGPSWLSIS 
Sbjct: 484  DIPTGENYVLKINYPFKHPPLPADLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISK 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSSCPGSQRVSWCKFEVI+DS KDVQISTSSSKTLEIP MIVTAINIKTIINE+QNVNEI
Sbjct: 544  FSSCPGSQRVSWCKFEVIIDSPKDVQISTSSSKTLEIPPMIVTAINIKTIINEKQNVNEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRN KA SN
Sbjct: 604  VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNSKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPS MWSKIGRL
Sbjct: 664  VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSCMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPGV+SCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK
Sbjct: 724  KRSVMPKLGKGGGIFGSGASPGVVSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E  PHDIPRM+ ASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLWGR
Sbjct: 784  DRKEVTPHDIPRMYHASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWGR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDK S+YVKEKK+VKKR N G EEK++D  D+DDA
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKISTYVKEKKMVKKRTNHGSEEKNLDNVDLDDA 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            N+E APN  SGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NIE-APNTESGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGV+PRLPSSK+TGVLPELLKNLVQRRRMVKSWMKNASG+KLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVIPRLPSSKVTGVLPELLKNLVQRRRMVKSWMKNASGIKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            DIG+VKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDG PYEVIER
Sbjct: 1084 DIGQVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGTPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDV ESIHDSL+KIQEDMRKGQVALEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVTESIHDSLVKIQEDMRKGQVALEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGI 2019
            ITKTLTKPPEAYPDARNQPHVQVA RLKQMGYSTGCSVGDTIPYIICCEQGSTSGGS GI
Sbjct: 1204 ITKTLTKPPEAYPDARNQPHVQVALRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSVGI 1263

Query: 2020 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQ 2079
            AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG+DSSKFQ
Sbjct: 1264 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFQ 1323

Query: 2080 IKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSE 2139
             KSSEVS SDVSSSLLCS+ND ERYQGC PLTLTCPSCSGTFECPAIFSSI KS +GK E
Sbjct: 1324 NKSSEVSRSDVSSSLLCSINDEERYQGCIPLTLTCPSCSGTFECPAIFSSIYKSADGKQE 1383

Query: 2140 RPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDET 2199
            +  VDEPT KFWN L CPKCPDEA+AGR+TPGMI+NQVKRQAERFIS+YYNGL+MCEDET
Sbjct: 1384 K-AVDEPTSKFWNNLRCPKCPDEASAGRMTPGMISNQVKRQAERFISMYYNGLLMCEDET 1443

Query: 2200 CKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEK 2259
            CKY TRAVNLR+MGD+EKGTICPNY HCNGRLIRKYTE DLYKQLAY+S+ LDT+RCMEK
Sbjct: 1444 CKYTTRAVNLRVMGDSEKGTICPNYTHCNGRLIRKYTEVDLYKQLAYFSHTLDTIRCMEK 1503

Query: 2260 LEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVTI 2305
            LEVHARVTLEKEMAKIRPIVELAASTIQS+RDRSAY WVQLQD  V +
Sbjct: 1504 LEVHARVTLEKEMAKIRPIVELAASTIQSLRDRSAYGWVQLQDFVVMV 1548

BLAST of Sgr021331 vs. NCBI nr
Match: XP_022947955.1 (DNA polymerase alpha catalytic subunit [Cucurbita moschata])

HSP 1 Score: 2722.6 bits (7056), Expect = 0.0e+00
Identity = 1385/1548 (89.47%), Postives = 1455/1548 (93.99%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS ANRRRSRGSEA ARLQALERLKAIR+GGRRSEAGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSAANRRRSRGSEATARLQALERLKAIRTGGRRSEAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKA    SDESDGE EKPKKRK+EKKE QP
Sbjct: 64   LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAGICFSDESDGEPEKPKKRKSEKKEAQP 123

Query: 880  KKPSS-SLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKPSS SLSAAAAMMGKQKLSSMFTSSIFRKT +DDKAKGLACDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSTSLSAAAAMMGKQKLSSMFTSSIFRKTGKDDKAKGLACDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA  IS+TFAP+ A+KCEG+ AQSLNL  GSEL+K T N NSGMT+   N 
Sbjct: 184  RERRRKGQIGATSISKTFAPVSAMKCEGIIAQSLNLTGGSELVKGTVNGNSGMTKDFTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LE V+A IE+ GNGETK+F+ K++L+S+++L  + QSHN S+K+DVIEDNMP VVETK+
Sbjct: 244  DLESVQADIEIQGNGETKKFDSKDNLDSEMNLVSVGQSHNPSIKDDVIEDNMPTVVETKS 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            E L+KKEPVCTLNA I++  KDPALSATAGWQAVRSEGSGN DSAA+ SE+KS FDID D
Sbjct: 304  EALVKKEPVCTLNAMISDV-KDPALSATAGWQAVRSEGSGNADSAADTSEDKSHFDIDAD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFY+VDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKN+QRC+YAIPSASFLHSD
Sbjct: 364  GSLPFYMVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNVQRCVYAIPSASFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EMLKLQ DAEQSQLS TDLRTKLQEVTAGLKNEIA+QLL LNV TFSMTPVKRKYAFER 
Sbjct: 424  EMLKLQNDAEQSQLSPTDLRTKLQEVTAGLKNEIAQQLLDLNVPTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIP GE+YVLKINYPFKHPPLP DLKGESFCALLGTHRSALELLLIKRKIKGPSWLSIS 
Sbjct: 484  DIPTGENYVLKINYPFKHPPLPADLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISK 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSSCPGSQRVSWCKFEVI+DS KDVQISTSSSKTLEIP MIVTAINIKTIINE+QNVNEI
Sbjct: 544  FSSCPGSQRVSWCKFEVIIDSPKDVQISTSSSKTLEIPPMIVTAINIKTIINEKQNVNEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRN KA SN
Sbjct: 604  VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNSKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPS MWSKIGRL
Sbjct: 664  VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSCMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK
Sbjct: 724  KRSVMPKLGKGGGIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E  PHDIPRM+ ASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLWGR
Sbjct: 784  DRKEVTPHDIPRMYHASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWGR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDK S+YVKEKK+VKKR N G EEK++D  D+DDA
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKFSTYVKEKKMVKKRTNHGSEEKNLDNVDLDDA 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            N+E APN  SGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NIE-APNTESGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGV+PRLPSSK+TGVLPELLKNLVQRRRMVKSWMKNASG+KLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVIPRLPSSKVTGVLPELLKNLVQRRRMVKSWMKNASGIKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            DIG+VKAIAGKVIQEVN+KYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDG PYEVIER
Sbjct: 1084 DIGQVKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGTPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDV ESIHDSL+KIQEDMRKGQV LEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVTESIHDSLVKIQEDMRKGQVVLEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGI 2019
            ITKTLTKPPEAYPDA+NQPHVQVA RLKQMGYSTGCSVGDTIPYIICCEQGSTSGGS GI
Sbjct: 1204 ITKTLTKPPEAYPDAKNQPHVQVALRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSVGI 1263

Query: 2020 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQ 2079
            AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG+DSSKFQ
Sbjct: 1264 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFQ 1323

Query: 2080 IKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSE 2139
             KSSEVS SDVSSSLLCS+ND ERYQGC PLTLTCPSCSGTFECPAIFSSI KS +GK E
Sbjct: 1324 NKSSEVSRSDVSSSLLCSINDEERYQGCIPLTLTCPSCSGTFECPAIFSSIYKSADGKQE 1383

Query: 2140 RPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDET 2199
            +  VDEPT KFWN L CPKCPDEA+AGR+TPGMIANQVKRQAERFIS+YYNGL+MCEDET
Sbjct: 1384 K-AVDEPTSKFWNNLRCPKCPDEASAGRMTPGMIANQVKRQAERFISMYYNGLLMCEDET 1443

Query: 2200 CKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEK 2259
            CKY TRAVNLR+MGD+EKGTICPNY HCNGRLIRKYTE DLYKQLAY+S+ LDT+RCMEK
Sbjct: 1444 CKYTTRAVNLRVMGDSEKGTICPNYTHCNGRLIRKYTEVDLYKQLAYFSHTLDTIRCMEK 1503

Query: 2260 LEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVTI 2305
            LEVHARVTLEKEMAKIRPIVELAASTIQS+RDRSAY WVQLQD  VT+
Sbjct: 1504 LEVHARVTLEKEMAKIRPIVELAASTIQSLRDRSAYGWVQLQDFVVTV 1548

BLAST of Sgr021331 vs. NCBI nr
Match: XP_038902720.1 (DNA polymerase alpha catalytic subunit [Benincasa hispida])

HSP 1 Score: 2704.9 bits (7010), Expect = 0.0e+00
Identity = 1379/1553 (88.80%), Postives = 1454/1553 (93.63%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS  NRRRSRGSEAAARL ALERLKAIRSGGRRS+AGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSATNRRRSRGSEAAARLSALERLKAIRSGGRRSDAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKA   SSDESDGEL+KPKKRK EKKE QP
Sbjct: 64   LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAGVCSSDESDGELDKPKKRKVEKKEAQP 123

Query: 880  KKP-SSSLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKP SSSLSAAAAMMGKQKLSSMFTSSIFRKT RDDKAKGLACDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSSSLSAAAAMMGKQKLSSMFTSSIFRKTGRDDKAKGLACDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA+PISRT   +PAVK EGLTAQSLNL  GSELIKDTEN NSG+  V+ N 
Sbjct: 184  RERRRKGQIGAIPISRTVVSVPAVKSEGLTAQSLNLTAGSELIKDTENGNSGINGVVTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LEPVRAG+EV GNGET+EF  KEDLNSQI+LDP+ Q  NSS+KEDVIED+ P++VETKA
Sbjct: 244  DLEPVRAGVEVQGNGETEEFYPKEDLNSQINLDPVEQLPNSSIKEDVIEDSKPIMVETKA 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            EPL+KKEPV TLNAKI+  ++DPALSATAGWQAVRSEGSG+V+S+AE +EEKSDFDID D
Sbjct: 304  EPLVKKEPVSTLNAKISN-ERDPALSATAGWQAVRSEGSGSVNSSAESAEEKSDFDIDAD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFYIVDAHEELFGANMGTVYLFGKVKAG+++HSCCVVVKNMQRCIYAIPSASFLHSD
Sbjct: 364  GSLPFYIVDAHEELFGANMGTVYLFGKVKAGNSFHSCCVVVKNMQRCIYAIPSASFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EML+L++DAEQSQLS  DLR KLQEVTAGLKNE+AKQLL LNVSTFSMTPVKRKYAFER 
Sbjct: 424  EMLELRKDAEQSQLSPADLRAKLQEVTAGLKNEVAKQLLDLNVSTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIPAGE+YVLKINYPFKHPPLP DLKGESFCALLGTHRSALELLL+KRKI GPSWLSISN
Sbjct: 484  DIPAGENYVLKINYPFKHPPLPADLKGESFCALLGTHRSALELLLVKRKITGPSWLSISN 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSSCP SQRVSWCKFEVIV S KDVQ STSSSK LEIPSM+VTAINIKTIINERQN+NEI
Sbjct: 544  FSSCPASQRVSWCKFEVIVGSPKDVQTSTSSSKILEIPSMVVTAINIKTIINERQNINEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKA SN
Sbjct: 604  VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            +LICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL
Sbjct: 664  ILICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPG+MSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK
Sbjct: 724  KRSVMPKLGKGGSIFGSGASPGLMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E  PH+IP+MFQASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLW R
Sbjct: 784  DRKEVTPHEIPKMFQASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWRR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDKTSSYVKEKK+VKKR N G EEK VDEFD+DD 
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKTSSYVKEKKMVKKRTNHGPEEKSVDEFDLDDP 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            NVE APN  SGKGKKGSSY+GGLVLEPKRGLYDKY+LLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NVE-APNTESGKGKKGSSYSGGLVLEPKRGLYDKYVLLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGVVP LPSSK+TGVLPELLKNLVQRRRMVKSWMKNA+GLKLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVVPLLPSSKVTGVLPELLKNLVQRRRMVKSWMKNATGLKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLV+NNLNLEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVKNNLNLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER
Sbjct: 1084 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCL+QILSGGSCEDVIESIHDSL KIQEDMRKGQVALEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLNQILSGGSCEDVIESIHDSLTKIQEDMRKGQVALEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTG- 2019
            ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQ    G  +  
Sbjct: 1204 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQVCGRGSRSNQ 1263

Query: 2020 -----IAQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGI 2079
                    RARHPDELKKEDGKWMIDI+YYLSQQIHPVVSRLCASIQGTSPERLADCLG+
Sbjct: 1264 FLFLTTQGRARHPDELKKEDGKWMIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLGL 1323

Query: 2080 DSSKFQIKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKS 2139
            DSSKFQ +S EVS +DVS+SLLCSVND ERYQGC PLTLTCPSCSGTF CP IFSSI KS
Sbjct: 1324 DSSKFQNRSIEVSRNDVSTSLLCSVNDEERYQGCTPLTLTCPSCSGTFNCPGIFSSIYKS 1383

Query: 2140 TNGKSERPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLM 2199
              GK E+P VDEPT KFWN L+CPKCPDEAN GR+TPGMIANQVKRQA+RFIS+YYNGLM
Sbjct: 1384 VEGKQEKP-VDEPTSKFWNNLNCPKCPDEANVGRMTPGMIANQVKRQADRFISLYYNGLM 1443

Query: 2200 MCEDETCKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDT 2259
            MC+DETCKYATRAVNLR+MGD+EKGTICPNYPHCNG L+RKYTEADLYKQL+Y+S++LDT
Sbjct: 1444 MCDDETCKYATRAVNLRVMGDSEKGTICPNYPHCNGHLVRKYTEADLYKQLSYFSHILDT 1503

Query: 2260 VRCMEKLEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVT 2304
             RCMEKLEVHAR+TLEKEMA+IRPIVELAA+TIQSIRDRSAY W+QLQ+  VT
Sbjct: 1504 ERCMEKLEVHARLTLEKEMARIRPIVELAATTIQSIRDRSAYGWMQLQNFVVT 1553

BLAST of Sgr021331 vs. ExPASy Swiss-Prot
Match: O48653 (DNA polymerase alpha catalytic subunit OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0868300 PE=2 SV=2)

HSP 1 Score: 1860.9 bits (4819), Expect = 0.0e+00
Identity = 989/1567 (63.11%), Postives = 1202/1567 (76.71%), Query Frame = 0

Query: 759  SESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYD 818
            +++ +   R R+RGSEA AR  ALERL+AIR GG R+ A   QV++E PIYDT+ E++Y 
Sbjct: 6    ADAGASGRRSRARGSEAVARSAALERLRAIRDGGARA-AAAVQVRIEAPIYDTVAEEDYA 65

Query: 819  ALVAKRREEARGFIVDDDGLGYGDEGEEEDWS-KAVARSSDE-SDGELEKPKKRK----A 878
            ALVA+RR++A  FIVDDDGLGY D+G EEDW+ + +  SSDE SDGE   P+KRK     
Sbjct: 66   ALVARRRKDAGAFIVDDDGLGYADDGREEDWTHRTIHSSSDEGSDGEDGAPRKRKQPRPQ 125

Query: 879  EKKEPQPKKPSSSLSAAAAMMGKQKLSSMFTSSIFRKTSRD-DKAKGLACDSIVDDVIAE 938
             K+ PQ    ++SLSAAAAMMGKQ+LSSMFTSS+FRK   D  +   LA DSIVDDVIAE
Sbjct: 126  SKRPPQQSAAAASLSAAAAMMGKQRLSSMFTSSVFRKPGSDRGRDSSLAADSIVDDVIAE 185

Query: 939  FAPDETDRERRRKGQIGALPISRTFAPIPA-VKCEGLTAQSLNLGSELIKDTENENSGMT 998
            FAPD+ DRE RR+       + R  AP PA      + A+++ + + +   ++N      
Sbjct: 186  FAPDDNDREERRR------RVGRVCAPAPAPTTTAHIKAENVAVDTAMAFRSDN------ 245

Query: 999  RVIANGELEPVRAGIEVLGNGETKEFEEKEDLNSQISLD-PIVQSHNSSVKEDVIEDNMP 1058
                      V    EV  +G   + E K D+  +  LD P+  S   +   + +E+   
Sbjct: 246  ----------VFEAHEVSDHGNDMDMELKPDVEMEPKLDTPLGASAELANNSNSLEE--- 305

Query: 1059 VVVETKAEPLLKKEPVCTLNAKI--NEAKKDPALSATAGWQAVRSEG--SGNVDSAAEIS 1118
               + +A   +K E V  LNAKI   +++     SATAGW  +  +G  +G   + A  S
Sbjct: 306  --PKQEANGEVKIEKVHRLNAKIKTEDSRNGDMASATAGWMKICGDGDNAGGEGAVAANS 365

Query: 1119 ----EEKSDFDIDTDGSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQ 1178
                +E S+F++  DG+LPFYI+DA+EE FGAN GTVYLFGKV+ G  +HSCCVVVKNMQ
Sbjct: 366  NTGVDESSEFEL-KDGALPFYILDAYEEPFGANSGTVYLFGKVEVGKRFHSCCVVVKNMQ 425

Query: 1179 RCIYAIPSASFLHSDEMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVST 1238
            RCIYAIPS+S    D + +L++++  S  S   LR  L E+ +GLK+EIA +L   NVS 
Sbjct: 426  RCIYAIPSSSIFPRDTISRLEKNSTTSD-SSPSLRASLHELASGLKSEIADKLSDFNVSN 485

Query: 1239 FSMTPVKRKYAFERVDIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLL 1298
            F+MTPVKR YAFER D+P GE YVLKINYP+K P LP DL+G+ F ALLGT+ SALELLL
Sbjct: 486  FAMTPVKRNYAFERTDLPNGEQYVLKINYPYKDPALPTDLRGQHFHALLGTNNSALELLL 545

Query: 1299 IKRKIKGPSWLSISNFSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAI 1358
            IKRKIKGPSWLSIS F +CP +QRVSWCKFEV VDS KD+ +  +S+ TLE+P ++V A+
Sbjct: 546  IKRKIKGPSWLSISKFLACPATQRVSWCKFEVTVDSPKDISVLMTST-TLEVPPVVVAAV 605

Query: 1359 NIKTIINERQNVNEIVSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMG 1418
            N+KTIINE+ NV+EIVSASVICC R KID PM + +W+K GML HFT++RKL+G IFP+G
Sbjct: 606  NLKTIINEKHNVHEIVSASVICCHRVKIDSPMRSEDWQKRGMLSHFTVMRKLEGSIFPIG 665

Query: 1419 FAKESTDRNLKARSNVLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQ 1478
             +KES+DRN KA SNVL  E +ERALLNRLMIEL KLD DVLVGHNISGFDLDVLLHRAQ
Sbjct: 666  LSKESSDRNQKAGSNVLALESSERALLNRLMIELSKLDCDVLVGHNISGFDLDVLLHRAQ 725

Query: 1479 FCRVPSSMWSKIGRLKRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKE 1538
             C+VPS+MWSKIGRL+RSVMP+L +G  ++GSGASPG+MSCIAGRLLCDTYL SRDLLKE
Sbjct: 726  TCKVPSNMWSKIGRLRRSVMPRLTKGNTLYGSGASPGIMSCIAGRLLCDTYLCSRDLLKE 785

Query: 1539 ISYSLTELAKTQLNKDRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLP 1598
            +SYSLT+LA+TQL K+R+E +PHDIP MFQ+S +L+ L+E GE DA L+LELMFHLSVLP
Sbjct: 786  VSYSLTQLAETQLKKERKEVSPHDIPPMFQSSGALLKLVEYGETDACLALELMFHLSVLP 845

Query: 1599 LTRQLTNISGNLWGRSLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMND 1658
            LTRQLTNISGNLWG++LQG+RAQRVEYLLLHAFHA+K+I+PDK +   KE    K++MN 
Sbjct: 846  LTRQLTNISGNLWGKTLQGSRAQRVEYLLLHAFHARKFIVPDKFAR-SKEFNSTKRKMNP 905

Query: 1659 GFEEKHVDEFD--IDDANVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNS 1718
              E    DE D  IDD       +   GK KKG SYAGGLVLEPK+GLYDKY+LLLDFNS
Sbjct: 906  DTEAARPDEADPSIDDE----GHHVDQGKTKKGPSYAGGLVLEPKKGLYDKYVLLLDFNS 965

Query: 1719 LYPSIIQEYNICFTTVERSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGL 1778
            LYPSIIQEYNICFTTV+RS DG VP LP+SK TGVLPELLK+LV+RRRMVKSW+K ASGL
Sbjct: 966  LYPSIIQEYNICFTTVDRSADGNVPNLPASKTTGVLPELLKSLVERRRMVKSWLKTASGL 1025

Query: 1779 KLQQLDIQQQALKLTANSMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNL 1838
            K QQ DIQQQALKLTANSMYGCLGFSNSRFYAKPLAELIT QGREILQ+TVDLVQNNLNL
Sbjct: 1026 KRQQFDIQQQALKLTANSMYGCLGFSNSRFYAKPLAELITLQGREILQNTVDLVQNNLNL 1085

Query: 1839 EVIYGDTDSIMIHSGLDDIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYA 1898
            EVIYGDTDSIMIH+GLDDI + K IAGKVIQEVNKKY+CLEIDLDG+YKRMLLLKKKKYA
Sbjct: 1086 EVIYGDTDSIMIHTGLDDISRAKGIAGKVIQEVNKKYRCLEIDLDGIYKRMLLLKKKKYA 1145

Query: 1899 AVKLQFKDGMPYEVIERKGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLM 1958
            A+K+   DG   E IERKGLDMVRRDWSLLSKE+GDFCL+QILSGGSC+DVIESIH SL+
Sbjct: 1146 AIKVAL-DGSLRENIERKGLDMVRRDWSLLSKEIGDFCLNQILSGGSCDDVIESIHSSLV 1205

Query: 1959 KIQEDMRKGQVALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIP 2018
            ++QE MR GQ  LEKYIITK+LTK PE YPDA+NQPHVQVA RLKQ GYS GCS GDT+P
Sbjct: 1206 QVQEQMRGGQTELEKYIITKSLTKAPEDYPDAKNQPHVQVALRLKQNGYS-GCSAGDTVP 1265

Query: 2019 YIICCEQGSTSGGSTGIAQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGT 2078
            YIIC +Q S S  S GIAQRARHP+ELK+   KWMIDIDYYLSQQIHPVVSRLCASIQGT
Sbjct: 1266 YIICSQQDSESTHSGGIAQRARHPEELKRNPDKWMIDIDYYLSQQIHPVVSRLCASIQGT 1325

Query: 2079 SPERLADCLGIDSSKFQIKSSEVSSSDVSSSLLCSVND-GERYQGCQPLTLTCPSCSGTF 2138
            SP RLA+CLG+DSSKFQ + +E  + D SS LL  ++D  ERY+GC+PL L+CPSCS TF
Sbjct: 1326 SPARLAECLGLDSSKFQSRLTESDNQDTSSMLLSVIDDEDERYRGCEPLRLSCPSCSTTF 1385

Query: 2139 ECPAIFSSICKSTNGKSERPIV-DEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQ 2198
            +CP + S I  S++G    P   ++ +  FW  + CP+CPD+ +  R++P ++ANQ+KRQ
Sbjct: 1386 DCPPVSSLIIGSSSGNVSNPNEGNDASINFWRRMRCPRCPDDTDESRVSPAVLANQMKRQ 1445

Query: 2199 AERFISVYYNGLMMCEDETCKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADL 2258
            A+ FI++YY GL+MC+DE CKY+T +VNLR+MGD+E+GTICPNYP CNG L+R+YTEADL
Sbjct: 1446 ADSFINLYYKGLLMCDDEGCKYSTHSVNLRVMGDSERGTICPNYPRCNGHLVRQYTEADL 1505

Query: 2259 YKQLAYYSYVLDTVRCMEKLEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQL 2305
            Y+QL+Y+ YV+D  RC+EKL+  AR+  EKE A +   + LA   +Q IRDR A+ WVQL
Sbjct: 1506 YRQLSYFCYVVDATRCLEKLDQKARLPFEKEFAALSQTINLALMEVQKIRDRCAFGWVQL 1534

BLAST of Sgr021331 vs. ExPASy Swiss-Prot
Match: Q9FHA3 (DNA polymerase alpha catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=POLA PE=3 SV=2)

HSP 1 Score: 1799.6 bits (4660), Expect = 0.0e+00
Identity = 944/1557 (60.63%), Postives = 1184/1557 (76.04%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRS-EAGGFQVKLENPIYDTIPEDEYD 819
            ++ +   RRRSRG+EA++R   LERLKAIR GG RS   GG+ ++L+ PI+DT+ ++EYD
Sbjct: 4    DNSTETGRRRSRGAEASSRKDTLERLKAIRQGGIRSASGGGYDIRLQKPIFDTVDDEEYD 63

Query: 820  ALVAKRREEARGFIVDD---DGLGYGDEGEEEDWSK-AVARSSDESD------GELEKPK 879
            ALV++RREEARGF+V+D     LGY DEGEEEDWSK +   S+DESD      G L+K K
Sbjct: 64   ALVSRRREEARGFVVEDGEGGDLGYLDEGEEEDWSKPSGPESTDESDDGGRFSGRLKKKK 123

Query: 880  KRKAEKKEPQPKKPSSSLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDV 939
            K K + ++PQ KK + +L AAA + G+ +LSSMFTSS F+K    DKA+    + I+D++
Sbjct: 124  KGKEQTQQPQVKKVNPALKAAATITGEGRLSSMFTSSSFKKVKETDKAQ---YEGILDEI 183

Query: 940  IAEFAPDETDRERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNLGSELIKDTENENSG 999
            IA+  PDE+DR++  + ++          P+   K + L + + ++G +           
Sbjct: 184  IAQVTPDESDRKKHTRRKLPGT------VPVTIFKNKKLFSVASSMGMK----------- 243

Query: 1000 MTRVIANGELEPVRAGIEVLGNGETKEFEEKEDL-NSQISLDPIVQSHNSSVKEDVIEDN 1059
                    E EP  +  E        E  ++ED+  S++     ++   S +   V ED 
Sbjct: 244  --------ESEPTPSTYEGDSVSMDNELMKEEDMKESEVIPSETMELLGSDI---VKEDG 303

Query: 1060 MPVVVETKAEPLLKKEPVCTLNAKINEAKKDPALSATAGW-QAVRSEGSGNVDSAAEISE 1119
               + +T+ +  L  + V TLNA I+  +KD ALSATAGW +A+   G+ N       SE
Sbjct: 304  SNKIRKTEVKSELGVKEVFTLNATIDMKEKDSALSATAGWKEAMGKVGTENGALLGSSSE 363

Query: 1120 EKSDFDIDTDGSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYA 1179
             K++FD+D DGSL F+I+DA+EE FGA+MGT+YLFGKVK GDTY SCCVVVKN+QRC+YA
Sbjct: 364  GKTEFDLDADGSLRFFILDAYEEAFGASMGTIYLFGKVKMGDTYKSCCVVVKNIQRCVYA 423

Query: 1180 IPSASFLHSDEMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTP 1239
            IP+ S   S E++ L+++ + S+LS    R KL E+ + LKNEIA++LL LNVS FSM P
Sbjct: 424  IPNDSIFPSHELIMLEQEVKDSRLSPESFRGKLHEMASKLKNEIAQELLQLNVSNFSMAP 483

Query: 1240 VKRKYAFERVDIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKI 1299
            VKR YAFER D+PAGE YVLKINY FK  PLP DLKGESF ALLG+H SALE  ++KRKI
Sbjct: 484  VKRNYAFERPDVPAGEQYVLKINYSFKDRPLPEDLKGESFSALLGSHTSALEHFILKRKI 543

Query: 1300 KGPSWLSISNFSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTI 1359
             GP WL IS+FS+C  S+ VSWCKFEV V S KD+ I  S  K +  P  +VTAIN+KTI
Sbjct: 544  MGPCWLKISSFSTCSPSEGVSWCKFEVTVQSPKDITILVSEEKVVH-PPAVVTAINLKTI 603

Query: 1360 INERQNVNEIVSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKES 1419
            +NE+QN++EIVSASV+C   AKID PM A E K+ G+L HFT++R  +G  +P+G+ KE 
Sbjct: 604  VNEKQNISEIVSASVLCFHNAKIDVPMPAPERKRSGILSHFTVVRNPEGTGYPIGWKKEV 663

Query: 1420 TDRNLKARSNVLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVP 1479
            +DRN K   NVL  E +ERALLNRL +EL KLDSD+LVGHNISGFDLDVLL RAQ C+V 
Sbjct: 664  SDRNSKNGCNVLSIENSERALLNRLFLELNKLDSDILVGHNISGFDLDVLLQRAQACKVQ 723

Query: 1480 SSMWSKIGRLKRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSL 1539
            SSMWSKIGRLKRS MPKL +G   +GSGA+PG+MSCIAGRLLCDT L SRDLLKE+SYSL
Sbjct: 724  SSMWSKIGRLKRSFMPKL-KGNSNYGSGATPGLMSCIAGRLLCDTDLCSRDLLKEVSYSL 783

Query: 1540 TELAKTQLNKDRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQL 1599
            T+L+KTQLN+DR+E  P+DIP+MFQ+S++LV+LIECGE DAWLS+ELMFHLSVLPLT QL
Sbjct: 784  TDLSKTQLNRDRKEIAPNDIPKMFQSSKTLVELIECGETDAWLSMELMFHLSVLPLTLQL 843

Query: 1600 TNISGNLWGRSLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEK 1659
            TNISGNLWG++LQGARAQR+EY LLH FH+KK+I+PDK S  +KE K  K+RM+   E++
Sbjct: 844  TNISGNLWGKTLQGARAQRIEYYLLHTFHSKKFILPDKISQRMKEIKSSKRRMDYAPEDR 903

Query: 1660 HVDEFDIDDANVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQ 1719
            +VDE D  D  +E  P+ GS K KKG +YAGGLVLEPKRGLYDKY+LLLDFNSLYPSIIQ
Sbjct: 904  NVDELDA-DLTLENDPSKGS-KTKKGPAYAGGLVLEPKRGLYDKYVLLLDFNSLYPSIIQ 963

Query: 1720 EYNICFTTVERSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDI 1779
            EYNICFTT+ RS DG VPRLPSS+  G+LP+L+++LV  R+ VK  MK  +GLK  +LDI
Sbjct: 964  EYNICFTTIPRSEDG-VPRLPSSQTPGILPKLMEHLVSIRKSVKLKMKKETGLKYWELDI 1023

Query: 1780 QQQALKLTANSMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDT 1839
            +QQALKLTANSMYGCLGFSNSRFYAKPLAELIT QGR+ILQ TVDLVQN+LNLEVIYGDT
Sbjct: 1024 RQQALKLTANSMYGCLGFSNSRFYAKPLAELITLQGRDILQRTVDLVQNHLNLEVIYGDT 1083

Query: 1840 DSIMIHSGLDDIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFK 1899
            DSIMIHSGLDDI +VKAI  KVIQEVNKKY+CL+ID DG+YKRMLLL+KKKYAAVKLQFK
Sbjct: 1084 DSIMIHSGLDDIEEVKAIKSKVIQEVNKKYRCLKIDCDGIYKRMLLLRKKKYAAVKLQFK 1143

Query: 1900 DGMPYEVIERKGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMR 1959
            DG P E IERKG+DMVRRDWSLLSKE+GD CLS+IL GGSCEDV+E+IH+ LMKI+E+MR
Sbjct: 1144 DGKPCEDIERKGVDMVRRDWSLLSKEIGDLCLSKILYGGSCEDVVEAIHNELMKIKEEMR 1203

Query: 1960 KGQVALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQ 2019
             GQVALEKY+ITKTLTKPP AYPD+++QPHVQVA R++Q GY  G +  DT+PYIIC EQ
Sbjct: 1204 NGQVALEKYVITKTLTKPPAAYPDSKSQPHVQVALRMRQRGYKEGFNAKDTVPYIICYEQ 1263

Query: 2020 G-STSGGSTGIAQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLA 2079
            G ++S  S GIA+RARHPDE+K E  +W++DIDYYL+QQIHPVVSRLCA IQGTSPERLA
Sbjct: 1264 GNASSASSAGIAERARHPDEVKSEGSRWLVDIDYYLAQQIHPVVSRLCAEIQGTSPERLA 1323

Query: 2080 DCLGIDSSKFQIKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFS 2139
            +CLG+D SK++ KS++ +SSD S+SLL + +D ERY+ C+PL LTCPSCS  F CP+I S
Sbjct: 1324 ECLGLDPSKYRSKSNDATSSDPSTSLLFATSDEERYKSCEPLALTCPSCSTAFNCPSIIS 1383

Query: 2140 SICKSTNGKSERPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVY 2199
            S+C S + K   P  +E    FW  L CPKC  E + G I+P MIANQVKRQ + F+S+Y
Sbjct: 1384 SVCASISKKPATPETEESDSTFWLKLHCPKCQQEDSTGIISPAMIANQVKRQIDGFVSMY 1443

Query: 2200 YNGLMMCEDETCKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYS 2259
            Y G+M+CEDE+CK+ TR+ N RL+G+ E+GT+CPNYP+CNG L+RKYTEADLYKQL+Y+ 
Sbjct: 1444 YKGIMVCEDESCKHTTRSPNFRLLGERERGTVCPNYPNCNGTLLRKYTEADLYKQLSYFC 1503

Query: 2260 YVLDTVRCMEKLEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAV 2303
            ++LDT   +EK++V  R+ +EK M KIRP V+ AA+  +S RDR AY W+QL D+ +
Sbjct: 1504 HILDTQCSLEKMDVGVRIQVEKAMTKIRPAVKSAAAITRSSRDRCAYGWMQLTDIVI 1524

BLAST of Sgr021331 vs. ExPASy Swiss-Prot
Match: Q9DE46 (DNA polymerase alpha catalytic subunit OS=Xenopus laevis OX=8355 GN=pola1 PE=1 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 2.2e-212
Identity = 563/1581 (35.61%), Postives = 827/1581 (52.31%), Query Frame = 0

Query: 758  VSESPS-VANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDE 817
            +S+S S  A+R R   +E + R +ALERLK  ++G    E   ++V+  + IY+ + E E
Sbjct: 1    MSDSGSFAASRSRREKTEKSGRKEALERLKRAKAG----EKVKYEVEQVSSIYEEVDEAE 60

Query: 818  YDALVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKE 877
            Y  LV  R+++   +IVDDDG GY ++G E      +     E +   +  K  K   K+
Sbjct: 61   YSKLVRDRQDD--DWIVDDDGTGYVEDGRE------IFDDDLEDNALADSGKGAKGAPKD 120

Query: 878  PQPKKPSSSLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDE 937
                K SS       +     + SMF +S  +KT+  DKA  L+ D ++ D++ +     
Sbjct: 121  KTNVKKSS-------VSKPNNIKSMFMASAVKKTT--DKAVDLSKDDLLGDLLQDLKSQA 180

Query: 938  TDRE-----RRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNLGSELIKDTENENSGMTR 997
                       +K ++   P++    P  A K    + + L   ++         + + R
Sbjct: 181  VPITPPPVITLKKKKLAGSPLNPFSVPPTAPKVLPTSVKRLPAVTKPGHPAAQSKASVPR 240

Query: 998  VIANGELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPV- 1057
             I   + EP    I         E + KE+ +  +  D      +  ++EDV  +  PV 
Sbjct: 241  QI---KKEPKAELISSAVGPLKVEAQVKEEDSGMVEFDD--GDFDEPMEEDV--EITPVD 300

Query: 1058 --VVETKAEPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEK 1117
               ++T+A+ +      C     I E K     SAT   ++   +         EI  + 
Sbjct: 301  SSTIKTQAQSI-----KCVKEENIKEEKSSFITSATLN-ESCWDQIDEAEPMTTEIQVDS 360

Query: 1118 SDFDIDT--DGS--LPFYIVDAHEELFGANMGTVYLFGKV--KAGDTYHSCCVVVKNMQR 1177
            S   + T  DGS    FY +DA+E+ + +  G VYLFGKV  ++ D Y SCCV VKN++R
Sbjct: 361  SHLPLVTGADGSQVFRFYWLDAYEDQY-SQPGVVYLFGKVWIESADAYVSCCVSVKNIER 420

Query: 1178 CIYAIPSASFLHSDEMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTF 1237
             +Y +P             +   + S    T     +  V       +A++     +  F
Sbjct: 421  TVYLLPR------------ENRVQLSTGKDTGAPVSMMHVYQEFNEAVAEK---YKIMKF 480

Query: 1238 SMTPVKRKYAFERVDIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLI 1297
                V + YAFE  D+PA   Y L++ Y    P LP DLKGE+F  + GT+ S+LEL L+
Sbjct: 481  KSKKVDKDYAFEIPDVPASSEY-LEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLL 540

Query: 1298 KRKIKGPSWLSISNFSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAIN 1357
             RKIKGPSWL I   S    SQ +SWCK E +V     V +     K L  P ++V +++
Sbjct: 541  SRKIKGPSWLEIK--SPQLSSQPMSWCKVEAVVTRPDQVSV----VKDLAPPPVVVLSLS 600

Query: 1358 IKTIINERQNVNEIVSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGF 1417
            +KT+ N + + NEIV+ + +      +D         +P    HF ++ KL+  IFP  +
Sbjct: 601  MKTVQNAKTHQNEIVAIAALVHHTFPLDKAP-----PQPPFQTHFCVLSKLNDCIFPYDY 660

Query: 1418 AKESTDRNLKARSNVLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQF 1477
             +    +N    +N+ I    ER LL   + ++ K+D DV+VGH+I GFDL+VLL R   
Sbjct: 661  NEAVKQKN----ANIEIAL-TERTLLGFFLAKIHKIDPDVIVGHDIYGFDLEVLLQRINS 720

Query: 1478 CRVPSSMWSKIGRLKRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEI 1537
            C+VP   WSKIGRL+RSVMPKL  GGR   SG +    +C  GR++CD  +S+++L++  
Sbjct: 721  CKVP--FWSKIGRLRRSVMPKL--GGR---SGFAERNAAC--GRIICDIEISAKELIRCK 780

Query: 1538 SYSLTELAKTQLNKDRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPL 1597
            SY L+EL    L  +R    P +I   +  S  L+ ++E    DA   L++M  L+VLPL
Sbjct: 781  SYHLSELVHQILKAERVVIPPENIRNAYNDSVHLLYMLENTWIDAKFILQIMCELNVLPL 840

Query: 1598 TRQLTNISGNLWGRSLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDG 1657
              Q+TNI+GN+  R+L G R++R EYLLLHAF    +I+PDK          V K+M   
Sbjct: 841  ALQITNIAGNVMSRTLMGGRSERNEYLLLHAFTENNFIVPDKP---------VFKKMQQT 900

Query: 1658 FEEKHVDEFDIDDANVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYP 1717
              E      D DD   +   N    K +K ++YAGGLVLEPK G YDK+ILLLDFNSLYP
Sbjct: 901  TVE------DNDDMGTDQNKN----KSRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYP 960

Query: 1718 SIIQEYNICFTTVERSPDGV--------VPRLPSSKM-TGVLPELLKNLVQRRRMVKSWM 1777
            SIIQEYNICFTTV R             +P LP S +  G+LP  ++ LV+RRR VK  M
Sbjct: 961  SIIQEYNICFTTVHREAPSTQKGEDQDEIPELPHSDLEMGILPREIRKLVERRRHVKQLM 1020

Query: 1778 KNAS---GLKLQQLDIQQQALKLTANSMYGCLGFSNSRFYAKPLAELITSQGREILQSTV 1837
            K       L L Q DI+Q+ALKLTANSMYGCLGFS SRFYAKPLA L+T QGREIL  T 
Sbjct: 1021 KQPDLNPDLYL-QYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTK 1080

Query: 1838 DLVQNNLNLEVIYGDTDSIMIHSGLDDIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRM 1897
            ++VQ  +NLEVIYGDTDSIMI++  +++ +V  +  +V  E+NK YK LEID+DG++K +
Sbjct: 1081 EMVQ-KMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEINKSYKLLEIDIDGIFKSL 1140

Query: 1898 LLLKKKKYAAVKLQ-FKDGMPYEVIERKGLDMVRRDWSLLSKELGDFCLSQILSGGSCED 1957
            LLLKKKKYAA+ ++   DG      E KGLD+VRRDW  L+K+ G++ +SQILS    + 
Sbjct: 1141 LLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKQAGNYVISQILSDQPRDS 1200

Query: 1958 VIESIHDSLMKIQEDMRKGQVALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYS 2017
            ++E+I   L +I E++  G V + +Y I K LTK P+ YPD ++ PHV VA  +   G  
Sbjct: 1201 IVENIQKKLTEIGENVTNGTVPITQYEINKALTKDPQDYPDKKSLPHVHVALWINSQG-G 1260

Query: 2018 TGCSVGDTIPYIICCEQGSTSGGSTGIAQRARHPDELKKEDGKWMIDIDYYLSQQIHPVV 2077
                 GDTI Y+IC       G +   +QRA   ++L+K++    ID  YYLSQQ+HPVV
Sbjct: 1261 RKVKAGDTISYVIC-----QDGSNLSASQRAYAQEQLQKQE-NLSIDTQYYLSQQVHPVV 1320

Query: 2078 SRLCASIQGTSPERLADCLGIDSSKFQIKSSEVSSSDVSSSLL---CSVNDGERYQGCQP 2137
            +R+C  I G     +A  LG+D S+F+         + + +LL     + D E+Y+ C+ 
Sbjct: 1321 ARICEPIDGIDSALIAMWLGLDPSQFR-AHRHYQQDEENDALLGGPSQLTDEEKYRDCER 1380

Query: 2138 LTLTCPSCSGTFECPAIFSSICKSTNGKSERPIVDEPTRKFWNTLSCPKCPDEANAGRIT 2197
                CP C GT     I+ ++   +       +  EP  K  +   C   P +       
Sbjct: 1381 FKFFCPKC-GT---ENIYDNVFDGSG------LQIEPGLKRCSKPECDASPLDYVI---- 1440

Query: 2198 PGMIANQVKRQAERFISVYYNGLMMCEDETCKYATRAVNLRLMGDAEKGTICPNYPHCNG 2257
               + N++     R+I  YY+G ++CE++TC+  TR + L     +  G IC     C+ 
Sbjct: 1441 --QVHNKLLLDIRRYIKKYYSGWLVCEEKTCQNRTRRLPLSF---SRNGPIC---QACSK 1454

Query: 2258 RLIR-KYTEADLYKQLAYYSYVLDTVRCMEK-LEVHARVTLEKEM-AKIRPIVELAASTI 2305
              +R +Y E  LY QL +Y ++ D    +EK +    R  L+K++  +     +   ST+
Sbjct: 1501 ATLRSEYPEKALYTQLCFYRFIFDWDYALEKVVSEQERGHLKKKLFQESENQYKKLKSTV 1454

BLAST of Sgr021331 vs. ExPASy Swiss-Prot
Match: P09884 (DNA polymerase alpha catalytic subunit OS=Homo sapiens OX=9606 GN=POLA1 PE=1 SV=2)

HSP 1 Score: 735.3 bits (1897), Expect = 2.1e-210
Identity = 547/1591 (34.38%), Postives = 829/1591 (52.11%), Query Frame = 0

Query: 755  NQTVSESPS-VANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIP 814
            + ++S+S S V++R R        R +ALERLK  ++G    E   ++V+    +Y+ + 
Sbjct: 7    DDSLSDSGSFVSSRARREKKSKKGRQEALERLKKAKAG----EKYKYEVEDFTGVYEEVD 66

Query: 815  EDEYDALVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAE 874
            E++Y  LV  R+++   +IVDDDG+GY ++G E           D  D  L+  +K K  
Sbjct: 67   EEQYSKLVQARQDD--DWIVDDDGIGYVEDGRE-------IFDDDLEDDALDADEKGKDG 126

Query: 875  KKEPQPKKPSSSLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEF- 934
            K   + K+    L    A+     + SMF +   +KT+  DKA  L+ D ++ D++ +  
Sbjct: 127  KARNKDKRNVKKL----AVTKPNNIKSMFIACAGKKTA--DKAVDLSKDGLLGDILQDLN 186

Query: 935  --APDETDRE---RRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNLGSELIKDTENENS 994
               P  T       ++K  IGA P   +     AV              ++      +  
Sbjct: 187  TETPQITPPPVMILKKKRSIGASPNPFSVHTATAVP-----------SGKIASPVSRKEP 246

Query: 995  GMTRVIANGELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDN 1054
             +T V       P++   E  G+    E  E+E            Q   +   ED   D 
Sbjct: 247  PLTPV-------PLKRA-EFAGDDVQVESTEEE------------QESGAMEFEDGDFDE 306

Query: 1055 MPVVVETKAEPLLKK------EPVCTLNAKINEAKKDPA-----LSATAGWQAVRSEGSG 1114
               V E   EP+  K      EP   +  + +  K   +     L   + W  +  EG  
Sbjct: 307  PMEVEEVDLEPMAAKAWDKESEPAEEVKQEADSGKGTVSYLGSFLPDVSCWD-IDQEGDS 366

Query: 1115 NVDSAAEISEEKSDFDI----DTDGSLPFYIVDAHEELFGANMGTVYLFGKV--KAGDTY 1174
            +  S  E+  + S   +    D +    FY +DA+E+ +    G V+LFGKV  ++ +T+
Sbjct: 367  SF-SVQEVQVDSSHLPLVKGADEEQVFHFYWLDAYEDQYN-QPGVVFLFGKVWIESAETH 426

Query: 1175 HSCCVVVKNMQRCIYAIPSASFLHSDEMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEI 1234
             SCCV+VKN++R +Y +P           +++ D    + + T +   +++V      +I
Sbjct: 427  VSCCVMVKNIERTLYFLPR----------EMKIDLNTGKETGTPI--SMKDVYEEFDEKI 486

Query: 1235 AKQLLGLNVSTFSMTPVKRKYAFERVDIPAGEHYVLKINYPFKHPPLPVDLKGESFCALL 1294
            A +     +  F   PV++ YAFE  D+P    Y L++ Y  + P LP DLKGE+F  + 
Sbjct: 487  ATK---YKIMKFKSKPVEKNYAFEIPDVPEKSEY-LEVKYSAEMPQLPQDLKGETFSHVF 546

Query: 1295 GTHRSALELLLIKRKIKGPSWLSISNFSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKT 1354
            GT+ S+LEL L+ RKIKGP WL + +      +Q VSWCK E +      V +     K 
Sbjct: 547  GTNTSSLELFLMNRKIKGPCWLEVKSPQLL--NQPVSWCKVEAMALKPDLVNV----IKD 606

Query: 1355 LEIPSMIVTAINIKTIINERQNVNEIVSASVICCQRAKIDGPMLATEWKKPGMLRHFTII 1414
            +  P ++V A ++KT+ N + + NEI++ + +      +D         KP    HF ++
Sbjct: 607  VSPPPLVVMAFSMKTMQNAKNHQNEIIAMAALVHHSFALDKAA-----PKPPFQSHFCVV 666

Query: 1415 RKLDGGIFPMGFAKESTDRNLKARSNVLICEGNERALLNRLMIELFKLDSDVLVGHNISG 1474
             K    IFP  F +    +N+K           ER LL   + ++ K+D D++VGHNI G
Sbjct: 667  SKPKDCIFPYAFKEVIEKKNVKVE-----VAATERTLLGFFLAKVHKIDPDIIVGHNIYG 726

Query: 1475 FDLDVLLHRAQFCRVPSSMWSKIGRLKRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCD 1534
            F+L+VLL R   C+ P   WSKIGRLKRS MPKL  GGR     +  G  +   GR++CD
Sbjct: 727  FELEVLLQRINVCKAPH--WSKIGRLKRSNMPKL--GGR-----SGFGERNATCGRMICD 786

Query: 1535 TYLSSRDLLKEISYSLTELAKTQLNKDRREANPHDIPRMFQASESLVDLIECGEADAWLS 1594
              +S+++L++  SY L+EL +  L  +R      +I  M+  S  L+ L+E    DA   
Sbjct: 787  VEISAKELIRCKSYHLSELVQQILKTERVVIPMENIQNMYSESSQLLYLLEHTWKDAKFI 846

Query: 1595 LELMFHLSVLPLTRQLTNISGNLWGRSLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVK 1654
            L++M  L+VLPL  Q+TNI+GN+  R+L G R++R E+LLLHAF+   YI+PDK      
Sbjct: 847  LQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKP 906

Query: 1655 EKKIVKKRMNDGFEEKHVDEFDIDDANVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDK 1714
            ++K+       G E++ +D              N   KG+K ++YAGGLVL+PK G YDK
Sbjct: 907  QQKL-------GDEDEEID-----------GDTNKYKKGRKKAAYAGGLVLDPKVGFYDK 966

Query: 1715 YILLLDFNSLYPSIIQEYNICFTTVER--------SPDG---VVPRLPSSKM-TGVLPEL 1774
            +ILLLDFNSLYPSIIQE+NICFTTV+R        + DG    +P LP   +  G+LP  
Sbjct: 967  FILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVTEDGEQEQIPELPDPSLEMGILPRE 1026

Query: 1775 LKNLVQRRRMVKSWMK--NASGLKLQQLDIQQQALKLTANSMYGCLGFSNSRFYAKPLAE 1834
            ++ LV+RR+ VK  MK  + +   + Q DI+Q+ALKLTANSMYGCLGFS SRFYAKPLA 
Sbjct: 1027 IRKLVERRKQVKQLMKQQDLNPDLILQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAA 1086

Query: 1835 LITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLDDIGKVKAIAGKVIQEVNKKY 1894
            L+T +GREIL  T ++VQ  +NLEVIYGDTDSIMI++   ++ +V  +  KV  EVNK Y
Sbjct: 1087 LVTYKGREILMHTKEMVQ-KMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLY 1146

Query: 1895 KCLEIDLDGLYKRMLLLKKKKYAAVKLQ-FKDGMPYEVIERKGLDMVRRDWSLLSKELGD 1954
            K LEID+DG++K +LLLKKKKYAA+ ++   DG      E KGLD+VRRDW  L+K+ G+
Sbjct: 1147 KLLEIDIDGVFKSLLLLKKKKYAALVVEPTSDGNYVTKQELKGLDIVRRDWCDLAKDTGN 1206

Query: 1955 FCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYIITKTLTKPPEAYPDARNQP 2014
            F + QILS  S + ++E+I   L++I E++  G V + ++ I K LTK P+ YPD ++ P
Sbjct: 1207 FVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLP 1266

Query: 2015 HVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGIAQRARHPDELKKEDGKWMI 2074
            HV VA  +   G       GDT+ Y+IC       G +   +QRA  P++L+K+D    I
Sbjct: 1267 HVHVALWINSQG-GRKVKAGDTVSYVIC-----QDGSNLTASQRAYAPEQLQKQD-NLTI 1326

Query: 2075 DIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQIKSSEVSSSDVSSSLL--- 2134
            D  YYL+QQIHPVV+R+C  I G     +A  LG+D ++F++        + + +LL   
Sbjct: 1327 DTQYYLAQQIHPVVARICEPIDGIDAVLIATWLGLDPTQFRV--HHYHKDEENDALLGGP 1386

Query: 2135 CSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSERPIVDEPTRKFWNTLS 2194
              + D E+Y+ C+     CP+C GT     I+ ++   +          EP+    + + 
Sbjct: 1387 AQLTDEEKYRDCERFKCPCPTC-GT---ENIYDNVFDGSGTDM------EPSLYRCSNID 1446

Query: 2195 CPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDETCKYATRAVNLRLMGDA 2254
            C   P            ++N++     RFI  YY+G ++CE+ TC+  TR + L+    +
Sbjct: 1447 CKASPLTFTV------QLSNKLIMDIRRFIKKYYDGWLICEEPTCRNRTRHLPLQF---S 1454

Query: 2255 EKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEKLEV-HARVTLEKEM-- 2301
              G +CP        L  +Y++  LY QL +Y Y+ D    +EKL   H +  L+K+   
Sbjct: 1507 RTGPLCP--ACMKATLQPEYSDKSLYTQLCFYRYIFDAECALEKLTTDHEKDKLKKQFFT 1454

BLAST of Sgr021331 vs. ExPASy Swiss-Prot
Match: O89042 (DNA polymerase alpha catalytic subunit (Fragment) OS=Rattus norvegicus OX=10116 GN=Pola1 PE=1 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 2.4e-206
Identity = 525/1549 (33.89%), Postives = 812/1549 (52.42%), Query Frame = 0

Query: 758  VSESPS-VANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDE 817
            VS+S S VA+R R        R +ALERLK  ++G    E   ++V+    +Y+ + E++
Sbjct: 16   VSDSGSFVASRARREKKSKKGRQEALERLKKAKAG----EKYKYEVEDLTSVYEEVDEEQ 75

Query: 818  YDALVAKRREEARGFIVDDDGLGYGDEGEE------EDWSKAVARSSDESDGELEKPKKR 877
            Y  LV  R+++   +IVDDDG+GY ++G E      ED   A+    + SDG+    +K 
Sbjct: 76   YSKLVQARQDD--DWIVDDDGIGYVEDGREIFDDDLED--DALDTCGEGSDGKAH--RKD 135

Query: 878  KAEKKEPQPKKPSSSLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIA 937
            + + K+P   KP++             + +MF +S  +KT+  DK   L+ D ++ D++ 
Sbjct: 136  RKDVKKPSVTKPNN-------------IKAMFIASAGKKTT--DKTVDLSKDDLLGDILQ 195

Query: 938  EFAPDETDRE------RRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNLGSELIKDTEN 997
            +   +            ++K   GA P   +     AV   G  A  ++     +     
Sbjct: 196  DLNTETPQIAPPPVLIPKKKRSTGASPNPFSVHTATAVP-SGKIASPVSRKEPPLTPVPL 255

Query: 998  ENSGMTRVIANGEL--EPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKED 1057
            + +     +A  E   +   +G+    +G+  E  + E+++ +  +   +    S   E 
Sbjct: 256  KRAEFAGDLAQPECPEDEQESGVIEFEDGDFDEPMDTEEVDEEEPVTAKIWDQESEPVEG 315

Query: 1058 VIEDNMPVVVETKAEPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAA 1117
            V  +  P   ET     L                 D  L   + W   + + +  +    
Sbjct: 316  VKHEADP---ETGTTSFL-----------------DSFLPDVSCWDIDQKDENSFLLQEV 375

Query: 1118 EISEEKSDF--DIDTDGSLPFYIVDAHEELFGANMGTVYLFGK--VKAGDTYHSCCVVVK 1177
            ++           D +    FY +DA+E+ +    G V+LFGK  V++  T+ SCCV+VK
Sbjct: 376  QVDSNHLPLVKGADDEQVFQFYWLDAYEDPYN-QPGVVFLFGKVWVESAKTHVSCCVMVK 435

Query: 1178 NMQRCIYAIPSASFLHSDEMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLN 1237
            N++R +Y +P           +++ D    + + T +  K  +V     ++I+ +     
Sbjct: 436  NIERTLYFLPR----------EMKIDLNTGKETATPITMK--DVYEEFDSKISAK---YK 495

Query: 1238 VSTFSMTPVKRKYAFERVDIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALE 1297
            +  F    V++ YAFE  D+P    Y L++ Y  + P LP +LKGE+F  + GT+ S+LE
Sbjct: 496  IMKFKSKIVEKNYAFEIPDVPEKSEY-LEVRYSAEVPQLPQNLKGETFSHVFGTNTSSLE 555

Query: 1298 LLLIKRKIKGPSWLSISNFSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIV 1357
            L L+ RKIKGP WL + N      +Q +SWCKFE +      V +     K +  P ++V
Sbjct: 556  LFLMNRKIKGPCWLEVKNPQLL--NQPISWCKFEAMALKPDLVNV----IKDVSPPPLVV 615

Query: 1358 TAINIKTIINERQNVNEIVSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIF 1417
             + ++KT+ N + + +EI++ + +      +D         KP    HF ++ K    IF
Sbjct: 616  MSFSMKTMQNVQNHQHEIIAMAALVHHNFPLDKAP-----PKPPFQTHFCVVSKPKDCIF 675

Query: 1418 PMGFAKESTDRNLKARSNVLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLH 1477
            P  F +    +N++           ER LL   + ++ KLD D+LVGHNI GF+L+VLL 
Sbjct: 676  PCAFKEVIKKKNMEVE-----VAATERTLLGFFLAKVHKLDPDILVGHNICGFELEVLLQ 735

Query: 1478 RAQFCRVPSSMWSKIGRLKRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDL 1537
            R   C+VP   WSKIGRL+RS MPKL       GS +  G  +   GR++CD  +S ++L
Sbjct: 736  RINECKVP--FWSKIGRLRRSNMPKL-------GSRSGFGERNATCGRMICDVEISVKEL 795

Query: 1538 LKEISYSLTELAKTQLNKDRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLS 1597
            +   SY L+EL +  L  +R      +I  M+     L+ L+E    DA   L++M  L+
Sbjct: 796  IHCKSYHLSELVQQILKTERIVIPTENIRNMYSEPSHLLYLLEHIWKDARFILQIMCELN 855

Query: 1598 VLPLTRQLTNISGNLWGRSLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKR 1657
            VLPL  Q+TNI+GN+  R+L G R++R E+LLLHAF+   YI+PDK       +   K +
Sbjct: 856  VLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDK-------QIFRKPQ 915

Query: 1658 MNDGFEEKHVDEFDIDDANVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFN 1717
               G E++ +D              N   KG+K ++YAGGLVL+PK G YDK+ILLLDFN
Sbjct: 916  QKPGDEDEEID-----------GDTNKYKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFN 975

Query: 1718 SLYPSIIQEYNICFTTVER-----------SPDGVVPRLPSSKM-TGVLPELLKNLVQRR 1777
            SLYPSIIQE+NICFTTV+R                +P LP   +  G+LP  ++ LV+RR
Sbjct: 976  SLYPSIIQEFNICFTTVQRVASETLKATEDEEQEQIPELPDPNLDMGILPREIRKLVERR 1035

Query: 1778 RMVKSWMK--NASGLKLQQLDIQQQALKLTANSMYGCLGFSNSRFYAKPLAELITSQGRE 1837
            + VK  MK  + +   + Q DI+Q+ALKLTANSMYGCLGFS SRFYAKPLA L+T +GRE
Sbjct: 1036 KQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGRE 1095

Query: 1838 ILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLDDIGKVKAIAGKVIQEVNKKYKCLEIDLD 1897
            IL  T ++VQ  +NLEVIYGDTDSIMI++   ++ +V  +  KV  EVNK YK LEID+D
Sbjct: 1096 ILMHTKEMVQ-KMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKNEVNKLYKLLEIDID 1155

Query: 1898 GLYKRMLLLKKKKYAAVKLQ-FKDGMPYEVIERKGLDMVRRDWSLLSKELGDFCLSQILS 1957
            G++K +LLLKKKKYAA+ ++   DG      E KGLD+VRRDW  L+K+ G+F + QILS
Sbjct: 1156 GVFKSLLLLKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILS 1215

Query: 1958 GGSCEDVIESIHDSLMKIQEDMRKGQVALEKYIITKTLTKPPEAYPDARNQPHVQVAQRL 2017
              S + ++E+I   L++I E++  G V + ++ I K LTK P+ YPD ++ PHV VA  +
Sbjct: 1216 DQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWI 1275

Query: 2018 KQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGIAQRARHPDELKKEDGKWMIDIDYYLSQ 2077
               G       GDT+ Y+IC       G +    QRA  P++L+K+D    ID  YYL+Q
Sbjct: 1276 NSQG-GRKVKAGDTVSYVIC-----QDGSNLPATQRAYAPEQLQKQD-NLAIDTQYYLAQ 1335

Query: 2078 QIHPVVSRLCASIQGTSPERLADCLGIDSSKFQIKSSEVSSSDVSSSLL---CSVNDGER 2137
            QIHPVV+R+C  I G     +A  LG+DS++F++   +    + + +LL     + D E+
Sbjct: 1336 QIHPVVARICEPIDGIDAVLIALWLGLDSTQFRV--HQYHKDEENDALLGGPAQLTDEEK 1395

Query: 2138 YQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSERPIVDEPTRKFWNTLSCPKCPDEA 2197
            Y+ C+     CPSC GT     I+ ++ + +       +  EP+    + + C   P   
Sbjct: 1396 YKDCEKFKCLCPSC-GT---ENIYDNVFEGSG------MDMEPSLNRCSNIDCKASPATF 1426

Query: 2198 NAGRITPGMIANQVKRQAERFISVYYNGLMMCEDETCKYATRAVNLRLMGDAEKGTICPN 2257
                     ++N++     R I  YY+G ++CE+ TC+   R + L     +  G +C  
Sbjct: 1456 MV------QLSNKLIMDIRRCIKKYYDGWLICEEPTCRNRIRRLPLHF---SRNGPLC-- 1426

Query: 2258 YPHCNGRLIR-KYTEADLYKQLAYYSYVLDTVRCMEKLEVHARVTLEKE 2269
             P C   ++R +Y++  LY QL +Y Y+ D    +EKL  H +  L+K+
Sbjct: 1516 -PACMKAVLRPEYSDKSLYTQLCFYRYIFDADCALEKLPEHEKDKLKKQ 1426

BLAST of Sgr021331 vs. ExPASy TrEMBL
Match: A0A6J1L1Z1 (DNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111499673 PE=3 SV=1)

HSP 1 Score: 2731.1 bits (7078), Expect = 0.0e+00
Identity = 1391/1548 (89.86%), Postives = 1458/1548 (94.19%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS ANRRRSRGSEA ARLQALERLKAIR+GGRRSEAGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSAANRRRSRGSEATARLQALERLKAIRTGGRRSEAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKA   SSDESDGE EKPKKRK+EKKE QP
Sbjct: 64   LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAGICSSDESDGEPEKPKKRKSEKKEAQP 123

Query: 880  KKPSS-SLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKPSS SLSAAAAMMGKQKLSSMFTSSIFRKT +DDKAKGLACDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSTSLSAAAAMMGKQKLSSMFTSSIFRKTGKDDKAKGLACDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA PIS+TFAP+PA+KCEG+ AQSLNL  GSEL+K T N NSGMT+   N 
Sbjct: 184  RERRRKGQIGATPISKTFAPVPAMKCEGVIAQSLNLTGGSELVKGTVNGNSGMTKDFTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LE VRA IE+ GNGETK+F+ K+DL+S+++L  + QSHN S+KEDVIEDNMP+VVETK+
Sbjct: 244  DLESVRADIEIQGNGETKKFDSKDDLDSEMNLVSVGQSHNPSIKEDVIEDNMPIVVETKS 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            E L+KKEPVCTLNA I++  KDPALSATAGWQAVRSEGSGN DSAA+ SE+KS FDID D
Sbjct: 304  EALVKKEPVCTLNATISDV-KDPALSATAGWQAVRSEGSGNADSAADTSEDKSHFDIDAD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFY+VDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKN+QRC+YAIPSA FLHSD
Sbjct: 364  GSLPFYMVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNVQRCVYAIPSAFFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EMLKLQ DAEQSQLS TDLRTKLQEVTAGLKNEIA+QLL LNV TFSMTPVKRKYAFER 
Sbjct: 424  EMLKLQNDAEQSQLSPTDLRTKLQEVTAGLKNEIAQQLLDLNVPTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIP GE+YVLKINYPFKHPPLP DLKGESFCALLGTHRSALELLLIKRKIKGPSWLSIS 
Sbjct: 484  DIPTGENYVLKINYPFKHPPLPADLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISK 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSSCPGSQRVSWCKFEVI+DS KDVQISTSSSKTLEIP MI TAINIKTIINE+QNVNEI
Sbjct: 544  FSSCPGSQRVSWCKFEVIIDSPKDVQISTSSSKTLEIPPMIATAINIKTIINEKQNVNEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRN KA SN
Sbjct: 604  VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNSKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPS MWSKIGRL
Sbjct: 664  VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSCMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQL+K
Sbjct: 724  KRSVMPKLGKGGGIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLSK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E  PHDIPRM+ ASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLWGR
Sbjct: 784  DRKEVTPHDIPRMYHASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWGR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDK S+YVKEKK+VKKR N G EEK++D  D+DDA
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKISTYVKEKKMVKKRTNHGSEEKNLDNVDLDDA 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            N+E APN  SGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NLE-APNTESGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGV+PRLPSSK+TGVLPELLKNLVQRRRMVKSWMKNASG+KLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVIPRLPSSKVTGVLPELLKNLVQRRRMVKSWMKNASGIKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            DIG+VKAIA KVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER
Sbjct: 1084 DIGQVKAIAVKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDV ESIHDSL+KIQEDMRKGQVALEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVTESIHDSLVKIQEDMRKGQVALEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGI 2019
            ITKTLTKPPEAYPDARNQPHVQVA RLKQMGYSTGCSVGDTIPYIICCEQGSTSGGS GI
Sbjct: 1204 ITKTLTKPPEAYPDARNQPHVQVALRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSVGI 1263

Query: 2020 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQ 2079
            AQRARHPDELKKEDGKWMIDI YYLSQQIHPVVSRLCASIQGTSPERLADCLG+DSSKFQ
Sbjct: 1264 AQRARHPDELKKEDGKWMIDIVYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFQ 1323

Query: 2080 IKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSE 2139
             KSSEVS SDVSSSLLCS+ND ERYQGC PLTLTCPSCSGTFECPAIFSSI KS +GK E
Sbjct: 1324 NKSSEVSRSDVSSSLLCSINDEERYQGCIPLTLTCPSCSGTFECPAIFSSIYKSADGKQE 1383

Query: 2140 RPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDET 2199
            +  VDEPT KFWN L CPKCPDEA+AGR+TPGMIANQVKRQAERFIS+YYNGL+MCEDET
Sbjct: 1384 K-AVDEPTSKFWNNLRCPKCPDEASAGRMTPGMIANQVKRQAERFISMYYNGLLMCEDET 1443

Query: 2200 CKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEK 2259
            CKYATRAVNLR+MGD+EKGTICPNY HCNGRLIRKYTE DLYKQLAY+S+ LDT+RCMEK
Sbjct: 1444 CKYATRAVNLRVMGDSEKGTICPNYTHCNGRLIRKYTEVDLYKQLAYFSHTLDTIRCMEK 1503

Query: 2260 LEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVTI 2305
            LEVHARVTLEKEMAKIRPIVELAASTIQS+RDRSAY WVQLQD  VT+
Sbjct: 1504 LEVHARVTLEKEMAKIRPIVELAASTIQSLRDRSAYGWVQLQDFVVTV 1548

BLAST of Sgr021331 vs. ExPASy TrEMBL
Match: A0A6J1G8C4 (DNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111451682 PE=3 SV=1)

HSP 1 Score: 2722.6 bits (7056), Expect = 0.0e+00
Identity = 1385/1548 (89.47%), Postives = 1455/1548 (93.99%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS ANRRRSRGSEA ARLQALERLKAIR+GGRRSEAGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSAANRRRSRGSEATARLQALERLKAIRTGGRRSEAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKA    SDESDGE EKPKKRK+EKKE QP
Sbjct: 64   LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAGICFSDESDGEPEKPKKRKSEKKEAQP 123

Query: 880  KKPSS-SLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKPSS SLSAAAAMMGKQKLSSMFTSSIFRKT +DDKAKGLACDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSTSLSAAAAMMGKQKLSSMFTSSIFRKTGKDDKAKGLACDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA  IS+TFAP+ A+KCEG+ AQSLNL  GSEL+K T N NSGMT+   N 
Sbjct: 184  RERRRKGQIGATSISKTFAPVSAMKCEGIIAQSLNLTGGSELVKGTVNGNSGMTKDFTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LE V+A IE+ GNGETK+F+ K++L+S+++L  + QSHN S+K+DVIEDNMP VVETK+
Sbjct: 244  DLESVQADIEIQGNGETKKFDSKDNLDSEMNLVSVGQSHNPSIKDDVIEDNMPTVVETKS 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            E L+KKEPVCTLNA I++  KDPALSATAGWQAVRSEGSGN DSAA+ SE+KS FDID D
Sbjct: 304  EALVKKEPVCTLNAMISDV-KDPALSATAGWQAVRSEGSGNADSAADTSEDKSHFDIDAD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFY+VDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKN+QRC+YAIPSASFLHSD
Sbjct: 364  GSLPFYMVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNVQRCVYAIPSASFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EMLKLQ DAEQSQLS TDLRTKLQEVTAGLKNEIA+QLL LNV TFSMTPVKRKYAFER 
Sbjct: 424  EMLKLQNDAEQSQLSPTDLRTKLQEVTAGLKNEIAQQLLDLNVPTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIP GE+YVLKINYPFKHPPLP DLKGESFCALLGTHRSALELLLIKRKIKGPSWLSIS 
Sbjct: 484  DIPTGENYVLKINYPFKHPPLPADLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISK 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSSCPGSQRVSWCKFEVI+DS KDVQISTSSSKTLEIP MIVTAINIKTIINE+QNVNEI
Sbjct: 544  FSSCPGSQRVSWCKFEVIIDSPKDVQISTSSSKTLEIPPMIVTAINIKTIINEKQNVNEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRN KA SN
Sbjct: 604  VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNSKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPS MWSKIGRL
Sbjct: 664  VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSCMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK
Sbjct: 724  KRSVMPKLGKGGGIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E  PHDIPRM+ ASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLWGR
Sbjct: 784  DRKEVTPHDIPRMYHASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWGR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDK S+YVKEKK+VKKR N G EEK++D  D+DDA
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKFSTYVKEKKMVKKRTNHGSEEKNLDNVDLDDA 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            N+E APN  SGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NIE-APNTESGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGV+PRLPSSK+TGVLPELLKNLVQRRRMVKSWMKNASG+KLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVIPRLPSSKVTGVLPELLKNLVQRRRMVKSWMKNASGIKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            DIG+VKAIAGKVIQEVN+KYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDG PYEVIER
Sbjct: 1084 DIGQVKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGTPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDV ESIHDSL+KIQEDMRKGQV LEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVTESIHDSLVKIQEDMRKGQVVLEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGI 2019
            ITKTLTKPPEAYPDA+NQPHVQVA RLKQMGYSTGCSVGDTIPYIICCEQGSTSGGS GI
Sbjct: 1204 ITKTLTKPPEAYPDAKNQPHVQVALRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSVGI 1263

Query: 2020 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQ 2079
            AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG+DSSKFQ
Sbjct: 1264 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFQ 1323

Query: 2080 IKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSE 2139
             KSSEVS SDVSSSLLCS+ND ERYQGC PLTLTCPSCSGTFECPAIFSSI KS +GK E
Sbjct: 1324 NKSSEVSRSDVSSSLLCSINDEERYQGCIPLTLTCPSCSGTFECPAIFSSIYKSADGKQE 1383

Query: 2140 RPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDET 2199
            +  VDEPT KFWN L CPKCPDEA+AGR+TPGMIANQVKRQAERFIS+YYNGL+MCEDET
Sbjct: 1384 K-AVDEPTSKFWNNLRCPKCPDEASAGRMTPGMIANQVKRQAERFISMYYNGLLMCEDET 1443

Query: 2200 CKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEK 2259
            CKY TRAVNLR+MGD+EKGTICPNY HCNGRLIRKYTE DLYKQLAY+S+ LDT+RCMEK
Sbjct: 1444 CKYTTRAVNLRVMGDSEKGTICPNYTHCNGRLIRKYTEVDLYKQLAYFSHTLDTIRCMEK 1503

Query: 2260 LEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVTI 2305
            LEVHARVTLEKEMAKIRPIVELAASTIQS+RDRSAY WVQLQD  VT+
Sbjct: 1504 LEVHARVTLEKEMAKIRPIVELAASTIQSLRDRSAYGWVQLQDFVVTV 1548

BLAST of Sgr021331 vs. ExPASy TrEMBL
Match: A0A5A7TSE8 (DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00700 PE=3 SV=1)

HSP 1 Score: 2691.8 bits (6976), Expect = 0.0e+00
Identity = 1369/1548 (88.44%), Postives = 1447/1548 (93.48%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS +NRRRSRGSEAAARL ALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSASNRRRSRGSEAAARLTALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKA   SSDESDGEL+KPKKRK  KKE QP
Sbjct: 64   LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAGVCSSDESDGELDKPKKRKVVKKETQP 123

Query: 880  KKP-SSSLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKP SSSL+AAAAMMGKQKLSSMFTSSIFRKT RDDKAKGLACDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSSSLTAAAAMMGKQKLSSMFTSSIFRKTGRDDKAKGLACDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA+PI RT   +PAVK EG TA+ LN    S+ IK+TEN NS MTRV+ N 
Sbjct: 184  RERRRKGQIGAIPILRTVTSVPAVKSEGFTARGLNSTGESDFIKETENGNSEMTRVVTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LE VR G+EV GNGETKEF+ KEDLNSQI+LDP+ Q  NSS+KEDV  D + + VETKA
Sbjct: 244  DLESVRGGVEVQGNGETKEFDSKEDLNSQINLDPVEQLPNSSIKEDVSGDGISIKVETKA 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            EPL+KKEPV TLNAKI+  ++DPALSATA WQAVRSEGSG+V+SAAE++EEKSDFD DTD
Sbjct: 304  EPLVKKEPVSTLNAKISN-ERDPALSATAEWQAVRSEGSGSVNSAAEMAEEKSDFDTDTD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFYI+DAHEELFG NMGTVYLFGKVKAGDT+HSCCVVVKNMQRCIYAIPSASFLHSD
Sbjct: 364  GSLPFYIIDAHEELFGTNMGTVYLFGKVKAGDTFHSCCVVVKNMQRCIYAIPSASFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EML+LQ+DAE+SQLS  DLR KLQEVTAGLKNE+AKQLL LNVSTFSMTPVKRKYAFER 
Sbjct: 424  EMLELQKDAEESQLSPADLRAKLQEVTAGLKNEMAKQLLDLNVSTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIPAGE+YVLKINYPFKHPPLP DLKGE FCALLGTHRSALELLLIKRKIKGPSWLSIS 
Sbjct: 484  DIPAGENYVLKINYPFKHPPLPADLKGELFCALLGTHRSALELLLIKRKIKGPSWLSISK 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSSCP SQRVSWCKFEVIVDS KDVQ STSSSK LEIPS++VTAINIKTIINERQNVNEI
Sbjct: 544  FSSCPASQRVSWCKFEVIVDSPKDVQTSTSSSKILEIPSVVVTAINIKTIINERQNVNEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VS SVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKA SN
Sbjct: 604  VSVSVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL
Sbjct: 664  VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPG+MSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK
Sbjct: 724  KRSVMPKLGKGGNIFGSGASPGLMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E  PH+I +M+QASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLWGR
Sbjct: 784  DRKEVTPHEIQKMYQASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWGR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDK SSYVKEKKIVKKR + G E+K+VDEFD+DD 
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKNSSYVKEKKIVKKRTSHGSEDKNVDEFDLDDG 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            NVE APN  SGKGKKG SY GGLVLEPKRGLYDKY+LLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NVE-APNTESGKGKKGPSYLGGLVLEPKRGLYDKYVLLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGVVP LPSSK+TGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVVPLLPSSKVTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLV+NNLNLEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVKNNLNLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            D+GKVKAIAGKVIQEVN+KYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER
Sbjct: 1084 DVGKVKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCL+QILSGGSCEDV+ESIHDSLMKIQEDMRKGQVALEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLNQILSGGSCEDVVESIHDSLMKIQEDMRKGQVALEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGI 2019
            ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGY+TGCSVGDTIPYIICCEQ STSGGSTGI
Sbjct: 1204 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYTTGCSVGDTIPYIICCEQESTSGGSTGI 1263

Query: 2020 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQ 2079
            AQRARHPDELKKEDGKWMIDI+YYLSQQIHPVVSRLCASIQGTSPERLADCLG+DSSKFQ
Sbjct: 1264 AQRARHPDELKKEDGKWMIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFQ 1323

Query: 2080 IKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSE 2139
             +S EVS SDVS+SLLCSVND ERYQGC PLTLTCPSCSGTF CP IFSSI KS +G  E
Sbjct: 1324 NRSIEVSRSDVSTSLLCSVNDEERYQGCTPLTLTCPSCSGTFNCPPIFSSIYKSADGNQE 1383

Query: 2140 RPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDET 2199
            R +VDEPT KFWN L CPKCPDEANAGRITP +IANQVKRQA+RFIS+YYNGLMMC+DET
Sbjct: 1384 R-LVDEPTSKFWNNLHCPKCPDEANAGRITPRIIANQVKRQADRFISMYYNGLMMCDDET 1443

Query: 2200 CKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEK 2259
            CKYATRA NLR+MGD+EKGTICPNYPHCNG L+RKYTEADLYKQL+Y+S++LDT RCMEK
Sbjct: 1444 CKYATRAANLRVMGDSEKGTICPNYPHCNGHLVRKYTEADLYKQLSYFSHILDTERCMEK 1503

Query: 2260 LEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVTI 2305
            LEV+ARVTLEKEMA IRP+VELAA TIQS+RDRSAY W+QLQ+  VT+
Sbjct: 1504 LEVNARVTLEKEMASIRPVVELAAMTIQSLRDRSAYGWMQLQNFVVTV 1548

BLAST of Sgr021331 vs. ExPASy TrEMBL
Match: A0A1S3C6X9 (DNA polymerase OS=Cucumis melo OX=3656 GN=LOC103497378 PE=3 SV=1)

HSP 1 Score: 2691.8 bits (6976), Expect = 0.0e+00
Identity = 1369/1548 (88.44%), Postives = 1447/1548 (93.48%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS +NRRRSRGSEAAARL ALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSASNRRRSRGSEAAARLTALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKA   SSDESDGEL+KPKKRK  KKE QP
Sbjct: 64   LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAGVCSSDESDGELDKPKKRKVVKKETQP 123

Query: 880  KKP-SSSLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKP SSSL+AAAAMMGKQKLSSMFTSSIFRKT RDDKAKGLACDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSSSLTAAAAMMGKQKLSSMFTSSIFRKTGRDDKAKGLACDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA+PI RT   +PAVK EG TA+ LN    S+ IK+TEN NS MTRV+ N 
Sbjct: 184  RERRRKGQIGAIPILRTVTSVPAVKSEGFTARGLNSTGESDFIKETENGNSEMTRVVTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LE VR G+EV GNGETKEF+ KEDLNSQI+LDP+ Q  NSS+KEDV  D + + VETKA
Sbjct: 244  DLESVRGGVEVQGNGETKEFDSKEDLNSQINLDPVEQLPNSSIKEDVSGDGISIKVETKA 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            EPL+KKEPV TLNAKI+  ++DPALSATA WQAVRSEGSG+V+SAAE++EEKSDFD DTD
Sbjct: 304  EPLVKKEPVSTLNAKISN-ERDPALSATAEWQAVRSEGSGSVNSAAEMAEEKSDFDTDTD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFYI+DAHEELFG NMGTVYLFGKVKAGDT+HSCCVVVKNMQRCIYAIPSASFLHSD
Sbjct: 364  GSLPFYIIDAHEELFGTNMGTVYLFGKVKAGDTFHSCCVVVKNMQRCIYAIPSASFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EML+LQ+DAE+SQLS  DLR KLQEVTAGLKNE+AKQLL LNVSTFSMTPVKRKYAFER 
Sbjct: 424  EMLELQKDAEESQLSPADLRAKLQEVTAGLKNEMAKQLLDLNVSTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIPAGE+YVLKINYPFKHPPLP DLKGE FCALLGTHRSALELLLIKRKIKGPSWLSIS 
Sbjct: 484  DIPAGENYVLKINYPFKHPPLPADLKGELFCALLGTHRSALELLLIKRKIKGPSWLSISK 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSSCP SQRVSWCKFEVIVDS KDVQ STSSSK LEIPS++VTAINIKTIINERQNVNEI
Sbjct: 544  FSSCPASQRVSWCKFEVIVDSPKDVQTSTSSSKILEIPSVVVTAINIKTIINERQNVNEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VS SVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKA SN
Sbjct: 604  VSVSVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL
Sbjct: 664  VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPG+MSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK
Sbjct: 724  KRSVMPKLGKGGNIFGSGASPGLMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E  PH+I +M+QASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLWGR
Sbjct: 784  DRKEVTPHEIQKMYQASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWGR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDK SSYVKEKKIVKKR + G E+K+VDEFD+DD 
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKNSSYVKEKKIVKKRTSHGSEDKNVDEFDLDDG 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            NVE APN  SGKGKKG SY GGLVLEPKRGLYDKY+LLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NVE-APNTESGKGKKGPSYLGGLVLEPKRGLYDKYVLLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGVVP LPSSK+TGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVVPLLPSSKVTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLV+NNLNLEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVKNNLNLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            D+GKVKAIAGKVIQEVN+KYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER
Sbjct: 1084 DVGKVKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCL+QILSGGSCEDV+ESIHDSLMKIQEDMRKGQVALEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLNQILSGGSCEDVVESIHDSLMKIQEDMRKGQVALEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGI 2019
            ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGY+TGCSVGDTIPYIICCEQ STSGGSTGI
Sbjct: 1204 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYTTGCSVGDTIPYIICCEQESTSGGSTGI 1263

Query: 2020 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQ 2079
            AQRARHPDELKKEDGKWMIDI+YYLSQQIHPVVSRLCASIQGTSPERLADCLG+DSSKFQ
Sbjct: 1264 AQRARHPDELKKEDGKWMIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFQ 1323

Query: 2080 IKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSE 2139
             +S EVS SDVS+SLLCSVND ERYQGC PLTLTCPSCSGTF CP IFSSI KS +G  E
Sbjct: 1324 NRSIEVSRSDVSTSLLCSVNDEERYQGCTPLTLTCPSCSGTFNCPPIFSSIYKSADGNQE 1383

Query: 2140 RPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDET 2199
            R +VDEPT KFWN L CPKCPDEANAGRITP +IANQVKRQA+RFIS+YYNGLMMC+DET
Sbjct: 1384 R-LVDEPTSKFWNNLHCPKCPDEANAGRITPRIIANQVKRQADRFISMYYNGLMMCDDET 1443

Query: 2200 CKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEK 2259
            CKYATRA NLR+MGD+EKGTICPNYPHCNG L+RKYTEADLYKQL+Y+S++LDT RCMEK
Sbjct: 1444 CKYATRAANLRVMGDSEKGTICPNYPHCNGHLVRKYTEADLYKQLSYFSHILDTERCMEK 1503

Query: 2260 LEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVTI 2305
            LEV+ARVTLEKEMA IRP+VELAA TIQS+RDRSAY W+QLQ+  VT+
Sbjct: 1504 LEVNARVTLEKEMASIRPVVELAAMTIQSLRDRSAYGWMQLQNFVVTV 1548

BLAST of Sgr021331 vs. ExPASy TrEMBL
Match: A0A0A0LPU1 (DNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_2G278160 PE=3 SV=1)

HSP 1 Score: 2683.3 bits (6954), Expect = 0.0e+00
Identity = 1367/1547 (88.36%), Postives = 1442/1547 (93.21%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 819
            E PS +NRRRSRGSEAAARL ALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA
Sbjct: 4    EQPSASNRRRSRGSEAAARLTALERLKAIRSGGRRSEAGGFQVKLENPIYDTIPEDEYDA 63

Query: 820  LVAKRREEARGFIVDDDGLGYGDEGEEEDWSKAVARSSDESDGELEKPKKRKAEKKEPQP 879
            LVAKRREE RGFIVDDDGLGYGDEGEEEDWSKA    SDESDGEL+KPKKRK  KKE QP
Sbjct: 64   LVAKRREEVRGFIVDDDGLGYGDEGEEEDWSKAGVCFSDESDGELDKPKKRKVVKKETQP 123

Query: 880  KKP-SSSLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDVIAEFAPDETD 939
            KKP SSSL+AAAAMMGKQKLSSMFTSSIFRKT RDDKAKGL CDSIVDDVIAEFAPDETD
Sbjct: 124  KKPSSSSLTAAAAMMGKQKLSSMFTSSIFRKTGRDDKAKGLGCDSIVDDVIAEFAPDETD 183

Query: 940  RERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNL--GSELIKDTENENSGMTRVIANG 999
            RERRRKGQIGA+PI RT   +PAVK EG TA+ LNL   S+ IKD EN NS  TRV+ N 
Sbjct: 184  RERRRKGQIGAIPILRTVTSVPAVKSEGFTARGLNLTGESDFIKDAENGNSETTRVVTNS 243

Query: 1000 ELEPVRAGIEVLGNGETKEFEEKEDLNSQISLDPIVQSHNSSVKEDVIEDNMPVVVETKA 1059
            +LE VR G+EV GNGETKEF+ K DLNSQI+LDP+ Q  NS +KEDV  D MP+ VETKA
Sbjct: 244  DLESVRGGVEVQGNGETKEFDSK-DLNSQINLDPVEQLPNSLIKEDVSGDTMPIKVETKA 303

Query: 1060 EPLLKKEPVCTLNAKINEAKKDPALSATAGWQAVRSEGSGNVDSAAEISEEKSDFDIDTD 1119
            EPL+KKEPV TLNAKI+  ++DPALSATA WQAVRSEGSG+V+SAAE++EEKS+FD DTD
Sbjct: 304  EPLVKKEPVSTLNAKISN-ERDPALSATAEWQAVRSEGSGSVNSAAEMAEEKSEFDTDTD 363

Query: 1120 GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYAIPSASFLHSD 1179
            GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDT+HSCCVVVKNMQRCIYAIPSASFLHSD
Sbjct: 364  GSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTFHSCCVVVKNMQRCIYAIPSASFLHSD 423

Query: 1180 EMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTPVKRKYAFERV 1239
            EML+LQ+DAE+SQLS  DLR KLQEVTAGLKNE+AKQLL LNVSTFSMTPVKRKYAFER 
Sbjct: 424  EMLELQKDAEESQLSPADLRAKLQEVTAGLKNEMAKQLLDLNVSTFSMTPVKRKYAFERQ 483

Query: 1240 DIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKIKGPSWLSISN 1299
            DIPAGE+YV+KINYPFKHPPLP DLKGE FCALLGTHRSALELLLIKRKIKGPSWLSIS 
Sbjct: 484  DIPAGENYVIKINYPFKHPPLPADLKGELFCALLGTHRSALELLLIKRKIKGPSWLSISK 543

Query: 1300 FSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTIINERQNVNEI 1359
            FSS P SQRVSWCKFEVIVDS KDVQ STSSSK LEIP MIVTAINIKTIINERQ+VNEI
Sbjct: 544  FSSRPASQRVSWCKFEVIVDSPKDVQTSTSSSKNLEIPPMIVTAINIKTIINERQSVNEI 603

Query: 1360 VSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKESTDRNLKARSN 1419
            VSASVICCQRAKIDGPMLATEWKKPGMLRHFT+IRKLDGGIFPMGFAKESTDRN KA SN
Sbjct: 604  VSASVICCQRAKIDGPMLATEWKKPGMLRHFTVIRKLDGGIFPMGFAKESTDRNSKAGSN 663

Query: 1420 VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 1479
            VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL
Sbjct: 664  VLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVPSSMWSKIGRL 723

Query: 1480 KRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 1539
            KRSVMPKLG+GG IFGSGASPG+MSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK
Sbjct: 724  KRSVMPKLGKGGNIFGSGASPGLMSCIAGRLLCDTYLSSRDLLKEISYSLTELAKTQLNK 783

Query: 1540 DRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQLTNISGNLWGR 1599
            DR+E   H+IP+M+QASESL++LIE GE DAWLSLELMFHLSVLPLTRQLTNISGNLWGR
Sbjct: 784  DRKEVTSHEIPKMYQASESLMNLIEYGETDAWLSLELMFHLSVLPLTRQLTNISGNLWGR 843

Query: 1600 SLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEKHVDEFDIDDA 1659
            SLQGARAQRVEYLLLHAFHAKKYI+PDK SSYVK+KKIVKKR N G EEK+VD+FD+DD 
Sbjct: 844  SLQGARAQRVEYLLLHAFHAKKYIVPDKNSSYVKDKKIVKKRTNHGSEEKNVDQFDLDDG 903

Query: 1660 NVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVE 1719
            NVE APN  SGKGKKG SY GGLVLEPKRGLYDKY+LLLDFNSLYPSIIQEYNICFTTVE
Sbjct: 904  NVE-APNTDSGKGKKGPSYLGGLVLEPKRGLYDKYVLLLDFNSLYPSIIQEYNICFTTVE 963

Query: 1720 RSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1779
            RSPDGV+P LPSS++TGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN
Sbjct: 964  RSPDGVIPPLPSSRVTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDIQQQALKLTAN 1023

Query: 1780 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDTDSIMIHSGLD 1839
            SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLV+NNL+LEVIYGDTDSIMIHSGLD
Sbjct: 1024 SMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVKNNLSLEVIYGDTDSIMIHSGLD 1083

Query: 1840 DIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1899
            D+GKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER
Sbjct: 1084 DVGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGMPYEVIER 1143

Query: 1900 KGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMRKGQVALEKYI 1959
            KGLDMVRRDWSLLSKELGDFCL+QILSGGSCEDV+ESIHDSLMKIQEDMRKGQVALEKYI
Sbjct: 1144 KGLDMVRRDWSLLSKELGDFCLNQILSGGSCEDVVESIHDSLMKIQEDMRKGQVALEKYI 1203

Query: 1960 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQGSTSGGSTGI 2019
            ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGY+TGCSVGDTIPYIICCEQ STSGGSTGI
Sbjct: 1204 ITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYTTGCSVGDTIPYIICCEQESTSGGSTGI 1263

Query: 2020 AQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLGIDSSKFQ 2079
            AQRARHPDELKKEDGKWMIDI+YYLSQQIHPVVSRLCASIQGTSPERLADCLG+DSSKFQ
Sbjct: 1264 AQRARHPDELKKEDGKWMIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLGLDSSKFQ 1323

Query: 2080 IKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFSSICKSTNGKSE 2139
             +S EVS SD+S+SLLCSVND ERYQGC PLT TCPSCSGTF CP IFSSI KS  GK E
Sbjct: 1324 NRSIEVSRSDISTSLLCSVNDEERYQGCTPLTFTCPSCSGTFNCPPIFSSIYKSAEGKQE 1383

Query: 2140 RPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVYYNGLMMCEDET 2199
            R +VDEPT KFWN L CPKCPDEANAGRITPGMIANQVKRQA+RFIS+YYNGLMMC+DET
Sbjct: 1384 R-LVDEPTTKFWNNLRCPKCPDEANAGRITPGMIANQVKRQADRFISMYYNGLMMCDDET 1443

Query: 2200 CKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYSYVLDTVRCMEK 2259
            CKYATRAVNLR+MGD+EKGTICPNYPHCNG L+RKYTEADLYKQL+Y+S++LDT RCMEK
Sbjct: 1444 CKYATRAVNLRVMGDSEKGTICPNYPHCNGHLVRKYTEADLYKQLSYFSHILDTERCMEK 1503

Query: 2260 LEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAVT 2304
            LEVHARVTLEKEMA IRP+VELAA+TIQSIRDRSAY WVQLQ+  VT
Sbjct: 1504 LEVHARVTLEKEMASIRPVVELAATTIQSIRDRSAYGWVQLQNFVVT 1546

BLAST of Sgr021331 vs. TAIR 10
Match: AT5G67100.1 (DNA-directed DNA polymerases )

HSP 1 Score: 1799.6 bits (4660), Expect = 0.0e+00
Identity = 944/1557 (60.63%), Postives = 1184/1557 (76.04%), Query Frame = 0

Query: 760  ESPSVANRRRSRGSEAAARLQALERLKAIRSGGRRS-EAGGFQVKLENPIYDTIPEDEYD 819
            ++ +   RRRSRG+EA++R   LERLKAIR GG RS   GG+ ++L+ PI+DT+ ++EYD
Sbjct: 4    DNSTETGRRRSRGAEASSRKDTLERLKAIRQGGIRSASGGGYDIRLQKPIFDTVDDEEYD 63

Query: 820  ALVAKRREEARGFIVDD---DGLGYGDEGEEEDWSK-AVARSSDESD------GELEKPK 879
            ALV++RREEARGF+V+D     LGY DEGEEEDWSK +   S+DESD      G L+K K
Sbjct: 64   ALVSRRREEARGFVVEDGEGGDLGYLDEGEEEDWSKPSGPESTDESDDGGRFSGRLKKKK 123

Query: 880  KRKAEKKEPQPKKPSSSLSAAAAMMGKQKLSSMFTSSIFRKTSRDDKAKGLACDSIVDDV 939
            K K + ++PQ KK + +L AAA + G+ +LSSMFTSS F+K    DKA+    + I+D++
Sbjct: 124  KGKEQTQQPQVKKVNPALKAAATITGEGRLSSMFTSSSFKKVKETDKAQ---YEGILDEI 183

Query: 940  IAEFAPDETDRERRRKGQIGALPISRTFAPIPAVKCEGLTAQSLNLGSELIKDTENENSG 999
            IA+  PDE+DR++  + ++          P+   K + L + + ++G +           
Sbjct: 184  IAQVTPDESDRKKHTRRKLPGT------VPVTIFKNKKLFSVASSMGMK----------- 243

Query: 1000 MTRVIANGELEPVRAGIEVLGNGETKEFEEKEDL-NSQISLDPIVQSHNSSVKEDVIEDN 1059
                    E EP  +  E        E  ++ED+  S++     ++   S +   V ED 
Sbjct: 244  --------ESEPTPSTYEGDSVSMDNELMKEEDMKESEVIPSETMELLGSDI---VKEDG 303

Query: 1060 MPVVVETKAEPLLKKEPVCTLNAKINEAKKDPALSATAGW-QAVRSEGSGNVDSAAEISE 1119
               + +T+ +  L  + V TLNA I+  +KD ALSATAGW +A+   G+ N       SE
Sbjct: 304  SNKIRKTEVKSELGVKEVFTLNATIDMKEKDSALSATAGWKEAMGKVGTENGALLGSSSE 363

Query: 1120 EKSDFDIDTDGSLPFYIVDAHEELFGANMGTVYLFGKVKAGDTYHSCCVVVKNMQRCIYA 1179
             K++FD+D DGSL F+I+DA+EE FGA+MGT+YLFGKVK GDTY SCCVVVKN+QRC+YA
Sbjct: 364  GKTEFDLDADGSLRFFILDAYEEAFGASMGTIYLFGKVKMGDTYKSCCVVVKNIQRCVYA 423

Query: 1180 IPSASFLHSDEMLKLQEDAEQSQLSHTDLRTKLQEVTAGLKNEIAKQLLGLNVSTFSMTP 1239
            IP+ S   S E++ L+++ + S+LS    R KL E+ + LKNEIA++LL LNVS FSM P
Sbjct: 424  IPNDSIFPSHELIMLEQEVKDSRLSPESFRGKLHEMASKLKNEIAQELLQLNVSNFSMAP 483

Query: 1240 VKRKYAFERVDIPAGEHYVLKINYPFKHPPLPVDLKGESFCALLGTHRSALELLLIKRKI 1299
            VKR YAFER D+PAGE YVLKINY FK  PLP DLKGESF ALLG+H SALE  ++KRKI
Sbjct: 484  VKRNYAFERPDVPAGEQYVLKINYSFKDRPLPEDLKGESFSALLGSHTSALEHFILKRKI 543

Query: 1300 KGPSWLSISNFSSCPGSQRVSWCKFEVIVDSSKDVQISTSSSKTLEIPSMIVTAINIKTI 1359
             GP WL IS+FS+C  S+ VSWCKFEV V S KD+ I  S  K +  P  +VTAIN+KTI
Sbjct: 544  MGPCWLKISSFSTCSPSEGVSWCKFEVTVQSPKDITILVSEEKVVH-PPAVVTAINLKTI 603

Query: 1360 INERQNVNEIVSASVICCQRAKIDGPMLATEWKKPGMLRHFTIIRKLDGGIFPMGFAKES 1419
            +NE+QN++EIVSASV+C   AKID PM A E K+ G+L HFT++R  +G  +P+G+ KE 
Sbjct: 604  VNEKQNISEIVSASVLCFHNAKIDVPMPAPERKRSGILSHFTVVRNPEGTGYPIGWKKEV 663

Query: 1420 TDRNLKARSNVLICEGNERALLNRLMIELFKLDSDVLVGHNISGFDLDVLLHRAQFCRVP 1479
            +DRN K   NVL  E +ERALLNRL +EL KLDSD+LVGHNISGFDLDVLL RAQ C+V 
Sbjct: 664  SDRNSKNGCNVLSIENSERALLNRLFLELNKLDSDILVGHNISGFDLDVLLQRAQACKVQ 723

Query: 1480 SSMWSKIGRLKRSVMPKLGRGGRIFGSGASPGVMSCIAGRLLCDTYLSSRDLLKEISYSL 1539
            SSMWSKIGRLKRS MPKL +G   +GSGA+PG+MSCIAGRLLCDT L SRDLLKE+SYSL
Sbjct: 724  SSMWSKIGRLKRSFMPKL-KGNSNYGSGATPGLMSCIAGRLLCDTDLCSRDLLKEVSYSL 783

Query: 1540 TELAKTQLNKDRREANPHDIPRMFQASESLVDLIECGEADAWLSLELMFHLSVLPLTRQL 1599
            T+L+KTQLN+DR+E  P+DIP+MFQ+S++LV+LIECGE DAWLS+ELMFHLSVLPLT QL
Sbjct: 784  TDLSKTQLNRDRKEIAPNDIPKMFQSSKTLVELIECGETDAWLSMELMFHLSVLPLTLQL 843

Query: 1600 TNISGNLWGRSLQGARAQRVEYLLLHAFHAKKYIIPDKTSSYVKEKKIVKKRMNDGFEEK 1659
            TNISGNLWG++LQGARAQR+EY LLH FH+KK+I+PDK S  +KE K  K+RM+   E++
Sbjct: 844  TNISGNLWGKTLQGARAQRIEYYLLHTFHSKKFILPDKISQRMKEIKSSKRRMDYAPEDR 903

Query: 1660 HVDEFDIDDANVEYAPNNGSGKGKKGSSYAGGLVLEPKRGLYDKYILLLDFNSLYPSIIQ 1719
            +VDE D  D  +E  P+ GS K KKG +YAGGLVLEPKRGLYDKY+LLLDFNSLYPSIIQ
Sbjct: 904  NVDELDA-DLTLENDPSKGS-KTKKGPAYAGGLVLEPKRGLYDKYVLLLDFNSLYPSIIQ 963

Query: 1720 EYNICFTTVERSPDGVVPRLPSSKMTGVLPELLKNLVQRRRMVKSWMKNASGLKLQQLDI 1779
            EYNICFTT+ RS DG VPRLPSS+  G+LP+L+++LV  R+ VK  MK  +GLK  +LDI
Sbjct: 964  EYNICFTTIPRSEDG-VPRLPSSQTPGILPKLMEHLVSIRKSVKLKMKKETGLKYWELDI 1023

Query: 1780 QQQALKLTANSMYGCLGFSNSRFYAKPLAELITSQGREILQSTVDLVQNNLNLEVIYGDT 1839
            +QQALKLTANSMYGCLGFSNSRFYAKPLAELIT QGR+ILQ TVDLVQN+LNLEVIYGDT
Sbjct: 1024 RQQALKLTANSMYGCLGFSNSRFYAKPLAELITLQGRDILQRTVDLVQNHLNLEVIYGDT 1083

Query: 1840 DSIMIHSGLDDIGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFK 1899
            DSIMIHSGLDDI +VKAI  KVIQEVNKKY+CL+ID DG+YKRMLLL+KKKYAAVKLQFK
Sbjct: 1084 DSIMIHSGLDDIEEVKAIKSKVIQEVNKKYRCLKIDCDGIYKRMLLLRKKKYAAVKLQFK 1143

Query: 1900 DGMPYEVIERKGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVIESIHDSLMKIQEDMR 1959
            DG P E IERKG+DMVRRDWSLLSKE+GD CLS+IL GGSCEDV+E+IH+ LMKI+E+MR
Sbjct: 1144 DGKPCEDIERKGVDMVRRDWSLLSKEIGDLCLSKILYGGSCEDVVEAIHNELMKIKEEMR 1203

Query: 1960 KGQVALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIICCEQ 2019
             GQVALEKY+ITKTLTKPP AYPD+++QPHVQVA R++Q GY  G +  DT+PYIIC EQ
Sbjct: 1204 NGQVALEKYVITKTLTKPPAAYPDSKSQPHVQVALRMRQRGYKEGFNAKDTVPYIICYEQ 1263

Query: 2020 G-STSGGSTGIAQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLA 2079
            G ++S  S GIA+RARHPDE+K E  +W++DIDYYL+QQIHPVVSRLCA IQGTSPERLA
Sbjct: 1264 GNASSASSAGIAERARHPDEVKSEGSRWLVDIDYYLAQQIHPVVSRLCAEIQGTSPERLA 1323

Query: 2080 DCLGIDSSKFQIKSSEVSSSDVSSSLLCSVNDGERYQGCQPLTLTCPSCSGTFECPAIFS 2139
            +CLG+D SK++ KS++ +SSD S+SLL + +D ERY+ C+PL LTCPSCS  F CP+I S
Sbjct: 1324 ECLGLDPSKYRSKSNDATSSDPSTSLLFATSDEERYKSCEPLALTCPSCSTAFNCPSIIS 1383

Query: 2140 SICKSTNGKSERPIVDEPTRKFWNTLSCPKCPDEANAGRITPGMIANQVKRQAERFISVY 2199
            S+C S + K   P  +E    FW  L CPKC  E + G I+P MIANQVKRQ + F+S+Y
Sbjct: 1384 SVCASISKKPATPETEESDSTFWLKLHCPKCQQEDSTGIISPAMIANQVKRQIDGFVSMY 1443

Query: 2200 YNGLMMCEDETCKYATRAVNLRLMGDAEKGTICPNYPHCNGRLIRKYTEADLYKQLAYYS 2259
            Y G+M+CEDE+CK+ TR+ N RL+G+ E+GT+CPNYP+CNG L+RKYTEADLYKQL+Y+ 
Sbjct: 1444 YKGIMVCEDESCKHTTRSPNFRLLGERERGTVCPNYPNCNGTLLRKYTEADLYKQLSYFC 1503

Query: 2260 YVLDTVRCMEKLEVHARVTLEKEMAKIRPIVELAASTIQSIRDRSAYCWVQLQDLAV 2303
            ++LDT   +EK++V  R+ +EK M KIRP V+ AA+  +S RDR AY W+QL D+ +
Sbjct: 1504 HILDTQCSLEKMDVGVRIQVEKAMTKIRPAVKSAAAITRSSRDRCAYGWMQLTDIVI 1524

BLAST of Sgr021331 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 340.5 bits (872), Expect = 1.1e-92
Identity = 176/355 (49.58%), Postives = 239/355 (67.32%), Query Frame = 0

Query: 1   MEWVRGDQLGSGNFATISLAILTKGFDQC-PPLIAVKTSVAISSASLRNEKQVLDQIGTC 60
           MEW+R + +G G+F+T+SLA  +    +  P L+AVK+S  + SA+LRNE+ VLD +G C
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 61  PQIITCFGDGYTIERDGQKRYNLLLEYANGGSLADKLKTHGGRLPESDVLRYTRAVLNGL 120
            +I+ CFG+G T+E +G++ YNL LEYA+GGSLAD++K+ G  LPE +V R+TR+++ GL
Sbjct: 61  SEIVRCFGEGRTVE-NGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGL 120

Query: 121 KYIHASGWVHCDIKLANILTFDNGGAKIADFGLAKKAGRKRNTAETEVKFEWRGTPLYMS 180
            +IH +G+ HCDIKL N+L F +G  KI+DFGLAK+          EV  E RGTPLYM+
Sbjct: 121 CHIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKR-------RSGEVCVEIRGTPLYMA 180

Query: 181 PESVNGDECEPPCDIWALGCAVVEMVTGKPAWDVQP--KSNIYALMIRIGAGDEVPQSPE 240
           PESVN  E E P DIWALGC+VVEM +GK AW ++    +N+ +L++RIG+GDEVP+ P 
Sbjct: 181 PESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPV 240

Query: 241 NLSDEGKDFLRKCFIKEPSKRWTAEMLLNHPFVAGDTVTLKQAEPPAESPRGPFDFPEFA 300
            LS+EGKDF+ KCF+K  ++RWTAEMLL+HPF+A D  + ++ E  + SPR PFDFP + 
Sbjct: 241 ELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEEDEACSVSPRNPFDFPGWN 300

Query: 301 SLPLSSDDASDERCFSSSNISAAVDSRLDFASAMSTIRQLVSEKPLDWSVSNSWV 353
           S+       +D   F S              S    I  LVSEK  DWSVS  WV
Sbjct: 301 SV---QSPVNDSVMFGSL-----------VGSPEERISGLVSEKVPDWSVSCDWV 333

BLAST of Sgr021331 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 336.7 bits (862), Expect = 1.5e-91
Identity = 179/363 (49.31%), Postives = 230/363 (63.36%), Query Frame = 0

Query: 1   MEWVRGDQLGSGNFATISLAILTKGFDQCPPLIAVKTSVAISSASLRNEKQVLDQIGTCP 60
           MEWVRG+ +G G F+T+S A  ++     P LIAVK++ A  +ASL NEK VLD +G CP
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  QIITCFGDGYTIERDGQKRYNLLLEYANGGSLADKLKTHGGR-LPESDVLRYTRAVLNGL 120
           +II C+G+  T+E +G++ +NLLLEYA+ GSLA  +K  GG  LPES V R+T +VL GL
Sbjct: 61  EIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 KYIHASGWVHCDIKLANILTFDNGGAKIADFGLAKKAGRKRNTAETEVKFEWRGTPLYMS 180
           ++IHA G+ HCDIKLANIL F++G  KIADFGLA +           V  E RGTPLYM+
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSV--EIRGTPLYMA 180

Query: 181 PESVNGDECEPPCDIWALGCAVVEMVTGKPAWDVQPKSNIYALMIRIGAGDEVPQSPENL 240
           PE VN +E     D+WALGCAVVEM +GK AW V+  S+  +L+IRIG GDE+P+ PE L
Sbjct: 181 PECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEML 240

Query: 241 SDEGKDFLRKCFIKEPSKRWTAEMLLNHPFVAGD---------TVTLKQAEPPAESPRGP 300
           S+EGKDFL KCF+K+P+KRWTAEMLLNH FV  D          V +K  +    SP+ P
Sbjct: 241 SEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCP 300

Query: 301 FDFPEFASLPLSSDDASDERCFSSSNISAAVDSRLDFASAMSTIRQLVSEKPLDWSVSNS 354
           F+F ++ S  L                    DS   F S +  +  LVS    DWSV  S
Sbjct: 301 FEFDDWDSFTL--------------------DSNPSFDSPVERLGSLVSGSIPDWSVGGS 340

BLAST of Sgr021331 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 326.6 bits (836), Expect = 1.6e-88
Identity = 174/369 (47.15%), Postives = 233/369 (63.14%), Query Frame = 0

Query: 1   MEWVRGDQLGSGNFATISLAILTKG-FDQCPPLIAVKTSVAISSASLRNEKQVLDQIG-T 60
           MEW+RG+ +G G F+T+SLA  +     + PPL+AVK++ +  +ASL NEK VLD +G  
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDD 60

Query: 61  CPQIITCFGDGYTIERDGQKRYNLLLEYANGGSLADKLKTHGGR-LPESDVLRYTRAVLN 120
           C +I+ CFG+  T+E +G++ +NL LEYA+ GSL   LK   G  +PES V R+T +VL 
Sbjct: 61  CNEIVRCFGEDRTVE-NGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLR 120

Query: 121 GLKYIHASGWVHCDIKLANILTFDNGGAKIADFGLAKKAGRKRNTAETEVKFEWRGTPLY 180
           GL++IHA+G+ HCD+KL NIL F +G  KIADFGLAK+ G   +        + RGTPLY
Sbjct: 121 GLRHIHANGFAHCDLKLGNILLFGDGAVKIADFGLAKRIG---DLTALNYGVQIRGTPLY 180

Query: 181 MSPESVNGDECEPPCDIWALGCAVVEMVTGKPAWDVQPKSNIYALMIRIGAGDEVPQSPE 240
           M+PESVN +E     D+WALGC VVEM +GK AW ++  SN  +L++RIG GDEVP  PE
Sbjct: 181 MAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPE 240

Query: 241 NLSDEGKDFLRKCFIKEPSKRWTAEMLLNHPFVAGDT-----------VTLKQAEPPAES 300
            LS++G+DFL KCF+K+P KRWTAEMLLNHPFV  D            V   + E  + S
Sbjct: 241 ELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVSTS 300

Query: 301 PRGPFDFPEFASLPLSSD--DASDERCFSSSNISAAVDSRLDFASAMSTIRQLVSEKPLD 354
           PR PF+FP++ S+   S   D+ DER                       +  LV++   D
Sbjct: 301 PRCPFEFPDWVSVSSGSQTIDSPDER-----------------------VASLVTDMIPD 342

BLAST of Sgr021331 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 221.1 bits (562), Expect = 9.3e-57
Identity = 120/271 (44.28%), Postives = 159/271 (58.67%), Query Frame = 0

Query: 3   WVRGDQLGSGNFATISLAILTKGFDQCPPLIAVKTSVAISSASLRNEKQVLDQIGTCPQI 62
           W+RG  +G G+ AT+SL I   G        AVK++   SSA L+ E+ +L ++ + P I
Sbjct: 6   WIRGPIIGRGSTATVSLGITNSG-----DFFAVKSAEFSSSAFLQREQSILSKLSS-PYI 65

Query: 63  ITCFGDGYTIERDGQKRYNLLLEYANGGSLADKLKTHGGRLPESDVLRYTRAVLNGLKYI 122
           +   G   T E D +  YNLL+EY +GGSL D +K  GG+LPE  +  YTR +L GL Y+
Sbjct: 66  VKYIGSNVTKEND-KLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYL 125

Query: 123 HASGWVHCDIKLANILTFDNGG--AKIADFGLAKKAGRKRNTAETEVKFEWRGTPLYMSP 182
           H  G VHCD+K  N++    GG  AKI D G AK       T E     E+ GTP +MSP
Sbjct: 126 HDQGIVHCDVKSQNVMI---GGEIAKIVDLGCAK-------TVEENENLEFSGTPAFMSP 185

Query: 183 ESVNGDECEPPCDIWALGCAVVEMVTGKPAWDVQPKSN-IYALMIRIGAGDEVPQSPENL 242
           E   G+E   P D+WALGC V+EM TG   W   P+ N + A + +IG   E P  P  L
Sbjct: 186 EVARGEEQSFPADVWALGCTVIEMATGSSPW---PELNDVVAAIYKIGFTGESPVIPVWL 245

Query: 243 SDEGKDFLRKCFIKEPSKRWTAEMLLNHPFV 271
           S++G+DFLRKC  K+P +RWT E LL HPF+
Sbjct: 246 SEKGQDFLRKCLRKDPKQRWTVEELLQHPFL 256