Sgr021313 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr021313
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	cytochrome P450 81D1-like
Location	tig00153654: 879875 .. 880987 (+)
RNA-Seq Expression	Sgr021313
Synteny	Sgr021313

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGCTGTTTTGCTTTATGTTTGTGTCTTTCTGATCTCTCTCCTTGTTGTTGTAAGATGTTTGCTGCGGTCTGGTCGGAATCTTCCACCGAGCCCACGTTTTTCTCTTCCTTTTATAGGTCATCTCCATCTCATCAAACATCCCATCCACCGAAGGCTTCACGAGCTTTCGGAGAAGTATGGTCATGTCTTCTCTCTTTGGTTTGGTTCACGCCTTGTTGTAGTTGTATCGTCTCCTTCTGCTGTACGTGAATGCTTCACAAAGAATGACATTGTTCTTGCCAATCGACCGTTTTTGAACACTGGAAAGCACTTGACGTATAACTTTACGGTATTGGTAGTTGCCCCTTATGGTGAGTATTGGCGTAACCTCCGTCGTATCAGCACTCTCGAGATTTTCTCAGCATCCCGACTTAATTTGTTCTCAGGATCCGACAAAATGAAGTCAAGCATTTGCTATGTAAATTATGTGGCAATTCGTTGGAAGATTCTATGTAGTGGAGCTTGAGCCAATGTTTTCAGATCTCATGTCCAATATTGTCATGAGAATGGTGGCTGGAAAGAGGTATTATGGAGATGATATGTTAGATGAAGAAAAAGCTAAGCAATTTAGAGAGATTGTAAAACGAGTCATGTTGCATGCTGGGGCAACCAATCCAGGGGATTTCATACCCATATGGAATTGGATTGATCCAACTGGTCTCGAGAAGAAGATAATAAAGGTTGGACAGGAAGCAGATGCAATCTTTCAGCTTTAATTGATGAGATTCGAAATAAAACAGAAGGTGGGAATACCATGATTCACCATTTACTTCACTTGCAGGGCACCCAGCCTGAACACTTTAGTGACCAAATTATCAAAGGCCTCATACATGTATATTTCGTTTTCACCATTTTTATGCTTTTTTTTCTCCATCTAGTGTCTCTCTCTCTAAGTACTCAATTCTTGCAGGTTATATTATTAGCAGGGATTGATACTGCAGCTGTGGCATTAGAATGGGCACTCTCTCACCTGCTCAACAACCCGGAGGCGATAAAGAAAGCGAGAATTGAGATAGAGTGTTCCATTGGACAAGAGCGTCTAGTCAACGAAGACGATTTGTCAATTTGA

mRNA sequence

ATGGATGCTGTTTTGCTTTATGTTTGTGTCTTTCTGATCTCTCTCCTTGTTGTTGTAAGATGTTTGCTGCGGTCTGGTCGGAATCTTCCACCGAGCCCACGTTTTTCTCTTCCTTTTATAGGTCATCTCCATCTCATCAAACATCCCATCCACCGAAGGCTTCACGAGCTTTCGGAGAAGTATGGTCATGTCTTCTCTCTTTGGTTTGGTTCACGCCTTGTTGTAGTTGTATCGTCTCCTTCTGCTGTACGTGAATGCTTCACAAAGAATGACATTGTTCTTGCCAATCGACCGTTTTTGAACACTGGAAAGCACTTGACGTATAACTTTACGGTATTGGTAGTTGCCCCTTATGGTGAGTATTGGCGTAACCTCCGTCGTATCAGCACTCTCGAGATTTTCTCAGCATCCCGACTTAATTTGTTCTCAGGATCCGACAAAATGAAATTCTATGTAGTGGAGCTTGAGCCAATGTTTTCAGATCTCATGTCCAATATTGTCATGAGAATGGTGGCTGGAAAGAGGTATTATGGAGATGATATGTTAGATGAAGAAAAAGCTAAGCAATTTAGAGAGATTGTAAAACGAGTCATGTTGCATGCTGGGGCAACCAATCCAGGGGATTTCATACCCATATGGAATTGGATTGATCCAACTGGTCTCGAGAAGAAGATAATAAAGGGCACCCAGCCTGAACACTTTAGTGACCAAATTATCAAAGGCCTCATACATGTTATATTATTAGCAGGGATTGATACTGCAGCTGTGGCATTAGAATGGGCACTCTCTCACCTGCTCAACAACCCGGAGGCGATAAAGAAAGCGAGAATTGAGATAGAGTGTTCCATTGGACAAGAGCGTCTAGTCAACGAAGACGATTTGTCAATTTGA

Coding sequence (CDS)

ATGGATGCTGTTTTGCTTTATGTTTGTGTCTTTCTGATCTCTCTCCTTGTTGTTGTAAGATGTTTGCTGCGGTCTGGTCGGAATCTTCCACCGAGCCCACGTTTTTCTCTTCCTTTTATAGGTCATCTCCATCTCATCAAACATCCCATCCACCGAAGGCTTCACGAGCTTTCGGAGAAGTATGGTCATGTCTTCTCTCTTTGGTTTGGTTCACGCCTTGTTGTAGTTGTATCGTCTCCTTCTGCTGTACGTGAATGCTTCACAAAGAATGACATTGTTCTTGCCAATCGACCGTTTTTGAACACTGGAAAGCACTTGACGTATAACTTTACGGTATTGGTAGTTGCCCCTTATGGTGAGTATTGGCGTAACCTCCGTCGTATCAGCACTCTCGAGATTTTCTCAGCATCCCGACTTAATTTGTTCTCAGGATCCGACAAAATGAAATTCTATGTAGTGGAGCTTGAGCCAATGTTTTCAGATCTCATGTCCAATATTGTCATGAGAATGGTGGCTGGAAAGAGGTATTATGGAGATGATATGTTAGATGAAGAAAAAGCTAAGCAATTTAGAGAGATTGTAAAACGAGTCATGTTGCATGCTGGGGCAACCAATCCAGGGGATTTCATACCCATATGGAATTGGATTGATCCAACTGGTCTCGAGAAGAAGATAATAAAGGGCACCCAGCCTGAACACTTTAGTGACCAAATTATCAAAGGCCTCATACATGTTATATTATTAGCAGGGATTGATACTGCAGCTGTGGCATTAGAATGGGCACTCTCTCACCTGCTCAACAACCCGGAGGCGATAAAGAAAGCGAGAATTGAGATAGAGTGTTCCATTGGACAAGAGCGTCTAGTCAACGAAGACGATTTGTCAATTTGA

Protein sequence

MDAVLLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEYWRNLRRISTLEIFSASRLNLFSGSDKMKFYVVELEPMFSDLMSNIVMRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKIIKGTQPEHFSDQIIKGLIHVILLAGIDTAAVALEWALSHLLNNPEAIKKARIEIECSIGQERLVNEDDLSI

Homology

[Close]

BLAST of Sgr021313 vs. NCBI nr
Match: XP_038901746.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 450.7 bits (1158), Expect = 1.0e-122
Identity = 232/347 (66.86%), Postives = 263/347 (75.79%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEK 60
           MD +L+Y+ +F+ISLL+ ++ LL  G+NLPPSP FSLP IGHLHL+KHPIH+ LH  S K
Sbjct: 1   MDNILVYIFLFVISLLLALKLLLPHGKNLPPSPLFSLPLIGHLHLLKHPIHQNLHNHSNK 60

Query: 61  YGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGE 120
           YG +FSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVL VAPYGE
Sbjct: 61  YGRIFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 YWRNLRRISTLEIFSASRLNLFSG--SDKMK-----------------FYVVELEPMFSD 180
            WR+LRRIST EIFS SRLNLFSG   D++K                 F VVE+EPM  D
Sbjct: 121 LWRSLRRISTCEIFSTSRLNLFSGIRQDEVKRLLCKLCSGNSVEELLLFSVVEVEPMLLD 180

Query: 181 LMSNIVMRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGL 240
             SNIVMRMVAGKRY+GDD+L+E +A +FR++VKRVM++AGATNPGDFIP+WNWIDPTGL
Sbjct: 181 FTSNIVMRMVAGKRYFGDDVLNEGQAGKFRDVVKRVMMYAGATNPGDFIPLWNWIDPTGL 240

Query: 241 EKKIIK---------------------------------GTQPEHFSDQIIKGLIHVILL 296
           EKKIIK                                  T+PE+FSDQIIKGLIHVILL
Sbjct: 241 EKKIIKVGQEADAIFQGLIDEIRTQEGGNTMIHHLLHLQTTRPEYFSDQIIKGLIHVILL 300

[Close]

BLAST of Sgr021313 vs. NCBI nr
Match: XP_011650030.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN63296.1 hypothetical protein Csa_022604 [Cucumis sativus])

HSP 1 Score: 431.4 bits (1108), Expect = 6.2e-117
Identity = 223/345 (64.64%), Postives = 256/345 (74.20%), Query Frame = 0


Query: 4   VLLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYGH 63
           +L+Y+  FLISLL+ ++ LL  G+NLPP+P FSLP IGHLHL+KHPIH+ LH LS+KYGH
Sbjct: 5   ILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYGH 64

Query: 64  VFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEYWR 123
           VFSL FGS LVVV+SSPSAVRECFTKNDI+LANRPFLNTGKHL+YNFTVL VAPYGE WR
Sbjct: 65  VFSLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELWR 124

Query: 124 NLRRISTLEIFSASRLNLFS--GSDKMK-----------------FYVVELEPMFSDLMS 183
            LRRIST EIFS S+LN FS    D++K                 F  VELEP+  D  S
Sbjct: 125 RLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFTS 184

Query: 184 NIVMRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKK 243
           NIVMRMV GKRY+GDD+LDE +A++FR++VKRVML+AGATNPGDFIP+WNWIDPTGLEKK
Sbjct: 185 NIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKK 244

Query: 244 IIK----------------------------------GTQPEHFSDQIIKGLIHVILLAG 296
           I+K                                   TQPE+FSDQIIKGLIHVI LAG
Sbjct: 245 IMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVIFLAG 304

[Close]

BLAST of Sgr021313 vs. NCBI nr
Match: XP_023512110.1 (cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 426.4 bits (1095), Expect = 2.0e-115
Identity = 223/341 (65.40%), Postives = 255/341 (74.78%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEK 60
           M  +LLY+ +F I+LL+ ++   R  +NLPP+P FSLP IGHLHL+KHPIHR LH+LS K
Sbjct: 1   MAHILLYIFLFPITLLLALKFFPRHAKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLSNK 60

Query: 61  YGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGE 120
           +GHVFSL FGSRLVVV+SSPSAVRECFT NDIVLANRPFLNTGKHLTYNFTVL VAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 YWRNLRRISTLEIFSASRLNLFSG--SDKMK----------FYVVELEPMFSDLMSNIVM 180
            WR LRRIST EIFS  RL+L SG   D++K            VVE++PM  DL SNIVM
Sbjct: 121 LWRTLRRISTREIFSTCRLDLSSGIRQDEVKRLLCKLCRGDTVVVEVQPMIMDLTSNIVM 180

Query: 181 RMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKIIK- 240
           RMVAGKRY+GDD+ DEE+A++FR++VKRVML+AGATNPGDFIP+WNWIDP+G EKKI+K 
Sbjct: 181 RMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKIMKV 240

Query: 241 ---------------------------------GTQPEHFSDQIIKGLIHVILLAGIDTA 296
                                             TQ E+FSDQIIKGLIHVILLAGIDT 
Sbjct: 241 GEEADAIFHGLIDEIRNQKGSGNTMIDHLLHLQTTQSEYFSDQIIKGLIHVILLAGIDTT 300

[Close]

BLAST of Sgr021313 vs. NCBI nr
Match: XP_022985606.1 (cytochrome P450 81D1-like [Cucurbita maxima])

HSP 1 Score: 426.4 bits (1095), Expect = 2.0e-115
Identity = 223/341 (65.40%), Postives = 255/341 (74.78%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEK 60
           M  +LLY+ +F I+LL+ ++   R G+NLPP+P FSLP IGHLHL+KHPIHR LH+LS K
Sbjct: 1   MAHILLYIFLFPITLLLALKFFPRHGKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLSNK 60

Query: 61  YGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGE 120
           +GHVFSL FGSRLVVVVSSPSAV ECFT NDIVLANRPFLNTGKHLTYNFTVL VAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVVSSPSAVHECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 YWRNLRRISTLEIFSASRLNLFSG--SDKMK----------FYVVELEPMFSDLMSNIVM 180
            WR LRRIST EIFS  RL+LF G   +++K            VVE++PM  DL SNIVM
Sbjct: 121 LWRTLRRISTREIFSTCRLDLFLGIRQEEVKRLLCKLCRGNTVVVEVQPMIMDLTSNIVM 180

Query: 181 RMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKIIK- 240
           RMVAGKRY+GDD+ DEE+A++FR++VKRVML+AGATNPGDFIP+WNWIDP+G EKKI+K 
Sbjct: 181 RMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKIMKV 240

Query: 241 ---------------------------------GTQPEHFSDQIIKGLIHVILLAGIDTA 296
                                             TQ E+FSDQIIKGLIHVILLAGIDT 
Sbjct: 241 GEEADAIFQGLIDEIRNQKGSGNTMIDHLLHLQTTQSEYFSDQIIKGLIHVILLAGIDTT 300

[Close]

BLAST of Sgr021313 vs. NCBI nr
Match: XP_022943756.1 (cytochrome P450 81D1-like [Cucurbita moschata])

HSP 1 Score: 422.9 bits (1086), Expect = 2.2e-114
Identity = 221/341 (64.81%), Postives = 254/341 (74.49%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEK 60
           M  +LLY+ +F I+ L+ ++   R  +NLPP+P FSLP IGHLHL+KHPIHR LH LS K
Sbjct: 1   MAHILLYIFLFPITFLLALKFFPRHVKNLPPTPLFSLPIIGHLHLLKHPIHRTLHHLSNK 60

Query: 61  YGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGE 120
           +GHVFSL FGSRLVVV+SSPSAVRECFT NDIVLANRPFLNTGKHLTYNFTVL VAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 YWRNLRRISTLEIFSASRLNLFSG--SDKMK----------FYVVELEPMFSDLMSNIVM 180
            WR LRRIST EIFS +RL+L SG   D++K            VVE++PM  DL SNIVM
Sbjct: 121 LWRTLRRISTREIFSTTRLDLSSGIRQDEVKRLLCKLCRDDNVVVEVQPMIMDLTSNIVM 180

Query: 181 RMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKIIK- 240
           RMVAGKRY+GDD+ DEE+A++FR++VKRVML+AGATNPGDFIP+WNWIDP+G EKKI+K 
Sbjct: 181 RMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKIMKV 240

Query: 241 ---------------------------------GTQPEHFSDQIIKGLIHVILLAGIDTA 296
                                             TQ E+FSDQIIKGLIHVILLAGIDT 
Sbjct: 241 GEEADAIFNGLIDEIRNQKGSGNTMIDHLLHLQATQSEYFSDQIIKGLIHVILLAGIDTT 300

[Close]

BLAST of Sgr021313 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.7e-80
Identity = 160/336 (47.62%), Postives = 215/336 (63.99%), Query Frame = 0


Query: 7   YVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYGHVFS 66
           ++ V L+S+ + +  + R  RNLPPSP  +LP IGHLHLI   +HR L++LS+KYG VFS
Sbjct: 10  FLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFS 69

Query: 67  LWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEYWRNLR 126
           L  G+RLV+VVSSP+A  ECFTKNDIV ANRP    GK++ YN+T +V +PYGE+WRNLR
Sbjct: 70  LQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLR 129

Query: 127 RISTLEIFSASRLNLFSG--SDKMK-------------FYVVELEPMFSDLMSNIVMRMV 186
           R++ +EIFSA  LN F     D++K             F  VE++   S+L  N+ MRMV
Sbjct: 130 RLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMV 189

Query: 187 AGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKI------ 246
           AGKRY+G D +D ++AK FR ++  V  HAGA+NPGDF+P   WID    EKK+      
Sbjct: 190 AGKRYFGQD-VDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSKISQE 249

Query: 247 ---------------------------IKGTQPEHFSDQIIKGLIHVILLAGIDTAAVAL 295
                                      ++ +QPE+++DQIIKG+I V+LLAG DT+AV +
Sbjct: 250 MDAFLQRLIHESRINKNNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTV 309

[Close]

BLAST of Sgr021313 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.9e-77
Identity = 153/341 (44.87%), Postives = 211/341 (61.88%), Query Frame = 0


Query: 11  FLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYGHVFSLWFG 70
           F+I++ ++++   R  +NLPP P  ++P IG+LH +KHP+HR    LS+ YG +FSLWFG
Sbjct: 12  FIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFG 71

Query: 71  SRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEYWRNLRRIST 130
           SRLVVVVSSPS   ECFTKNDI+LANRP   TGK++ YN+T L  A YG++WRNLRRI+T
Sbjct: 72  SRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 131

Query: 131 LEIFSASRLNLFSG------------------SDKMKFYVVELEPMFSDLMSNIVMRMVA 190
           +++ S +RLN F G                  S+   F  VEL P  +++  N +MRM++
Sbjct: 132 IDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMIS 191

Query: 191 GKRYYGD--DMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKI----- 250
           GKRYYGD  D+ D E+AKQFREI+  +M   GA N GDF+P+   +D   LEK+      
Sbjct: 192 GKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIAK 251

Query: 251 -------------------------------IKGTQPEHFSDQIIKGLIHVILLAGIDTA 296
                                          +  +QPE++SD +IKGLI  +LLAG DT+
Sbjct: 252 RSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTS 311

[Close]

BLAST of Sgr021313 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 2.8e-75
Identity = 158/342 (46.20%), Postives = 210/342 (61.40%), Query Frame = 0


Query: 5   LLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYGHV 64
           L+    FLI  L V     R  +NLPP P+  LP IG+LH +K P+H   H LS+KYG +
Sbjct: 8   LIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTLSQKYGQI 67

Query: 65  FSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEYWRN 124
           FSLWFGSRLVVVVSS +  +ECFTKNDIVLANRP   TGK++ YN T +  +PYG++WRN
Sbjct: 68  FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 127

Query: 125 LRRISTLEIFSASRLNLF---------------SGSDKMKFYVVELEPMFSDLMSNIVMR 184
           LRRI ++EI S+ RLN F               +      F  VEL PMFS++  N +MR
Sbjct: 128 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMR 187

Query: 185 MVAGKRYYGD--DMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKIIK 244
           MV+GKRYYG+  D+ D E+A+ FR I+K V+   GA N GDF+    W D  GLEK++ K
Sbjct: 188 MVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKK 247

Query: 245 ----------------------------------GTQPEHFSDQIIKGLIHVILLAGIDT 296
                                              +QPE+++DQIIKGL+ V+LLAG DT
Sbjct: 248 ISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDT 307

[Close]

BLAST of Sgr021313 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 273.9 bits (699), Expect = 2.2e-72
Identity = 149/343 (43.44%), Postives = 210/343 (61.22%), Query Frame = 0


Query: 5   LLYVCVFLISLLVVVRCLLRSG--RNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYG 64
           LL   VF ++L  +   ++R+   +NLPP P  SLP IG+LH +K P+HR    LSEKYG
Sbjct: 6   LLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGLSEKYG 65

Query: 65  HVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEYW 124
           HVFSLWFGSRLVVVVSS S  ++CFTKND+VLANRP   +GK++ YN+T L    YGE+W
Sbjct: 66  HVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHW 125

Query: 125 RNLRRISTLEIFSASRLNLFSG---------------SDKMKFYVVELEPMFSDLMSNIV 184
           RNLRRI+ L++ S  R+N FSG                    F  +EL     D+  N +
Sbjct: 126 RNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNI 185

Query: 185 MRMVAGKRYYGD--DMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKI 244
           MRM++GKRYYG+  D  D ++A QFR++V  ++  +GA N  DF+P+  ++D   LEK++
Sbjct: 186 MRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRL 245

Query: 245 ----------------------------------IKGTQPEHFSDQIIKGLIHVILLAGI 295
                                             ++ +QPE+++DQIIKGL   +LLAG 
Sbjct: 246 KDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGT 305

[Close]

BLAST of Sgr021313 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.6e-70
Identity = 148/346 (42.77%), Postives = 213/346 (61.56%), Query Frame = 0


Query: 4   VLLYVC--VFLISLLVVVRCLLRSG--RNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSE 63
           +L Y+C  +F +S+  ++R L +S   +NLPP P  SLP IG+LH +K P++R    L+E
Sbjct: 3   ILSYLCYSLFYLSIFFIIRLLFQSRKFKNLPPGPT-SLPIIGNLHHLKRPLNRTFKALTE 62

Query: 64  KYGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYG 123
           KYG+V SLWFGSRLVVVVSS S  +ECFTKND+VLANRP   +GK++ YN+T L    YG
Sbjct: 63  KYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 122

Query: 124 EYWRNLRRISTLEIFSASRLNLF---------------SGSDKMKFYVVELEPMFSDLMS 183
           E+WRNLRRI++L++ S  R+N F               +     KF  VEL   F D+  
Sbjct: 123 EHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTF 182

Query: 184 NIVMRMVAGKRYYGD--DMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLE 243
           N +MRM++GKRYYGD  D+ + ++A QFR++V  ++  +GA N  DF+P+  ++D   LE
Sbjct: 183 NNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLE 242

Query: 244 KKI----------------------------------IKGTQPEHFSDQIIKGLIHVILL 295
           K++                                  ++ +QPE+++D IIKGL   +LL
Sbjct: 243 KRVKRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLL 302

[Close]

BLAST of Sgr021313 vs. ExPASy TrEMBL
Match: A0A0A0LTD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.0e-117
Identity = 223/345 (64.64%), Postives = 256/345 (74.20%), Query Frame = 0


Query: 4   VLLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYGH 63
           +L+Y+  FLISLL+ ++ LL  G+NLPP+P FSLP IGHLHL+KHPIH+ LH LS+KYGH
Sbjct: 5   ILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYGH 64

Query: 64  VFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEYWR 123
           VFSL FGS LVVV+SSPSAVRECFTKNDI+LANRPFLNTGKHL+YNFTVL VAPYGE WR
Sbjct: 65  VFSLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELWR 124

Query: 124 NLRRISTLEIFSASRLNLFS--GSDKMK-----------------FYVVELEPMFSDLMS 183
            LRRIST EIFS S+LN FS    D++K                 F  VELEP+  D  S
Sbjct: 125 RLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFTS 184

Query: 184 NIVMRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKK 243
           NIVMRMV GKRY+GDD+LDE +A++FR++VKRVML+AGATNPGDFIP+WNWIDPTGLEKK
Sbjct: 185 NIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKK 244

Query: 244 IIK----------------------------------GTQPEHFSDQIIKGLIHVILLAG 296
           I+K                                   TQPE+FSDQIIKGLIHVI LAG
Sbjct: 245 IMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVIFLAG 304

[Close]

BLAST of Sgr021313 vs. ExPASy TrEMBL
Match: A0A6J1J8Q1 (cytochrome P450 81D1-like OS=Cucurbita maxima OX=3661 GN=LOC111483619 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 9.7e-116
Identity = 223/341 (65.40%), Postives = 255/341 (74.78%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEK 60
           M  +LLY+ +F I+LL+ ++   R G+NLPP+P FSLP IGHLHL+KHPIHR LH+LS K
Sbjct: 1   MAHILLYIFLFPITLLLALKFFPRHGKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLSNK 60

Query: 61  YGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGE 120
           +GHVFSL FGSRLVVVVSSPSAV ECFT NDIVLANRPFLNTGKHLTYNFTVL VAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVVSSPSAVHECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 YWRNLRRISTLEIFSASRLNLFSG--SDKMK----------FYVVELEPMFSDLMSNIVM 180
            WR LRRIST EIFS  RL+LF G   +++K            VVE++PM  DL SNIVM
Sbjct: 121 LWRTLRRISTREIFSTCRLDLFLGIRQEEVKRLLCKLCRGNTVVVEVQPMIMDLTSNIVM 180

Query: 181 RMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKIIK- 240
           RMVAGKRY+GDD+ DEE+A++FR++VKRVML+AGATNPGDFIP+WNWIDP+G EKKI+K 
Sbjct: 181 RMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKIMKV 240

Query: 241 ---------------------------------GTQPEHFSDQIIKGLIHVILLAGIDTA 296
                                             TQ E+FSDQIIKGLIHVILLAGIDT 
Sbjct: 241 GEEADAIFQGLIDEIRNQKGSGNTMIDHLLHLQTTQSEYFSDQIIKGLIHVILLAGIDTT 300

[Close]

BLAST of Sgr021313 vs. ExPASy TrEMBL
Match: A0A6J1FSK8 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448412 PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 1.1e-114
Identity = 221/341 (64.81%), Postives = 254/341 (74.49%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEK 60
           M  +LLY+ +F I+ L+ ++   R  +NLPP+P FSLP IGHLHL+KHPIHR LH LS K
Sbjct: 1   MAHILLYIFLFPITFLLALKFFPRHVKNLPPTPLFSLPIIGHLHLLKHPIHRTLHHLSNK 60

Query: 61  YGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGE 120
           +GHVFSL FGSRLVVV+SSPSAVRECFT NDIVLANRPFLNTGKHLTYNFTVL VAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 YWRNLRRISTLEIFSASRLNLFSG--SDKMK----------FYVVELEPMFSDLMSNIVM 180
            WR LRRIST EIFS +RL+L SG   D++K            VVE++PM  DL SNIVM
Sbjct: 121 LWRTLRRISTREIFSTTRLDLSSGIRQDEVKRLLCKLCRDDNVVVEVQPMIMDLTSNIVM 180

Query: 181 RMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKIIK- 240
           RMVAGKRY+GDD+ DEE+A++FR++VKRVML+AGATNPGDFIP+WNWIDP+G EKKI+K 
Sbjct: 181 RMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKIMKV 240

Query: 241 ---------------------------------GTQPEHFSDQIIKGLIHVILLAGIDTA 296
                                             TQ E+FSDQIIKGLIHVILLAGIDT 
Sbjct: 241 GEEADAIFNGLIDEIRNQKGSGNTMIDHLLHLQATQSEYFSDQIIKGLIHVILLAGIDTT 300

[Close]

BLAST of Sgr021313 vs. ExPASy TrEMBL
Match: A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 6.5e-104
Identity = 206/345 (59.71%), Postives = 242/345 (70.14%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLLRSGR-NLPPSPRFSLPFIGHLHLIKHPIHRRLHELSE 60
           MD + L + + L SLL+    L RS R NLPPSP FSLP +GHLHL+KHP+H+  HELS 
Sbjct: 1   MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60

Query: 61  KYGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYG 120
           KYGHVFSL FGSR+VVVVSSPSAV+ECFTKNDIVLANRP L +GK L YN T + VAPYG
Sbjct: 61  KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120

Query: 121 EYWRNLRRISTLEIFSASRLNLFSG--SDKMK-------------FYVVELEPMFSDLMS 180
           E+WRNLRRI TLEIFS +RLN+F G   D+++             F  VELEPM  DL  
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180

Query: 181 NIVMRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKK 240
           NIVMRMVAGK+YY     +E+++K+FRE+V +V+ H GATNPGDFIPIWNWIDPTG EK+
Sbjct: 181 NIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 240

Query: 241 IIK----------------------------------GTQPEHFSDQIIKGLIHVILLAG 296
           IIK                                    +PE++SDQIIKGLI VILLAG
Sbjct: 241 IIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAG 300

[Close]

BLAST of Sgr021313 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 4.0e-101
Identity = 199/345 (57.68%), Postives = 239/345 (69.28%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLLRS-GRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSE 60
           MD +LL + + L SL +    LLR   RNLPPSP F LP IGHLHL+KHP+HR LH+LS 
Sbjct: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140

Query: 61  KYGHVFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYG 120
            YGHVFSL +GSRLVVVVSSPSAV+ECFTKNDIVLANRP L +GK L YN T L V+PYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200

Query: 121 EYWRNLRRISTLEIFSASRLNLFSG--SDKMK-------------FYVVELEPMFSDLMS 180
           E+WRNLRRISTLEIFS SRLNLF G   D++K             F  VEL+ M  D   
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260

Query: 181 NIVMRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKK 240
           NIV+RM+AGK+YYG+D+ DE ++K+FRE+VK ++ + G  N GDF+P+WNWIDPTG EK+
Sbjct: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320

Query: 241 IIK----------------------------------GTQPEHFSDQIIKGLIHVILLAG 296
           I+K                                   TQPE+FSDQIIKGL  ++LLAG
Sbjct: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380

[Close]

BLAST of Sgr021313 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 260.4 bits (664), Expect = 1.8e-69
Identity = 144/345 (41.74%), Postives = 207/345 (60.00%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLLRSGR--NLPPSPRFSLPFIGHLHLIKHPIHRRLHELS 60
           MD   + +  FL   + +   L +S R  NLPPSP + LP IGHLHL+K P+HR  H +S
Sbjct: 1   MDLTQILLLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSIS 60

Query: 61  EKYGH--VFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVA 120
           +  G+  +F L  G+RLV V+SS S   ECFTKND+VLANRP +   KH+ YNFT ++ A
Sbjct: 61  KSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAA 120

Query: 121 PYGEYWRNLRRISTLEIFSASRLNLFSG--SDKMK-------------FYVVELEPMFSD 180
            YG++WRNLRRI+ +EIFS+ R++ FS    D+++             F  VEL+ + ++
Sbjct: 121 SYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTN 180

Query: 181 LMSNIVMRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGL 240
           L  N ++ MVAGKRYYG    D ++AK  RE++  +M  AG+ N  D++P  NW+     
Sbjct: 181 LAFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWVTNFEN 240

Query: 241 EKKII--------------------KG------------TQPEHFSDQIIKGLIHVILLA 295
           + KI+                    KG            T+PE+++D IIKG+I  ++LA
Sbjct: 241 QTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLA 300

[Close]

BLAST of Sgr021313 vs. TAIR 10
Match: AT4G37400.1 (cytochrome P450, family 81, subfamily F, polypeptide 3 )

HSP 1 Score: 255.4 bits (651), Expect = 5.7e-68
Identity = 139/339 (41.00%), Postives = 209/339 (61.65%), Query Frame = 0


Query: 7   YVCVFLISLLVVVRCLLRSGR----NLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYG 66
           YV V  ++L ++   L  + +    NLPPSP +SLP +GH +L+K P+HR  H LS+ +G
Sbjct: 4   YVIVLPLALFLLAYKLFFTSKTKRFNLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHG 63

Query: 67  HVFSLWFGSRLVVVVSSPSAVRECFT-KNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEY 126
            +FSL FGSR  VV+SS S   +CFT +NDI+L+NRP   T K++ YN+T +  APYG++
Sbjct: 64  PIFSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDH 123

Query: 127 WRNLRRISTLEIFSASRLNLFSGSDKMKFY------------VVELEPMFSDLMSNIVMR 186
           WRNLRRI +LEI S++RL  F    K + +             +ELEP+ SDL  N ++R
Sbjct: 124 WRNLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVNKEIELEPLLSDLTFNNIVR 183

Query: 187 MVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKI---- 246
           MV GKRYYGD++ +EE+A  F+++V  +   +GA +PGD++P       +  EKK+    
Sbjct: 184 MVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS-FEKKVKALA 243

Query: 247 -----------------------------IKGTQPEHFSDQIIKGLIHVILLAGIDTAAV 296
                                        ++  +PE+++D  IKGL+  +++AG DT+AV
Sbjct: 244 EAMDEILQRLLEECKRDKDGNTMVNHLLSLQQNEPEYYTDVTIKGLMLGMMIAGTDTSAV 303

[Close]

BLAST of Sgr021313 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 8.3e-67
Identity = 145/348 (41.67%), Postives = 203/348 (58.33%), Query Frame = 0


Query: 1   MDAVLLYVCVFLISLLVVVRCLL---RSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHEL 60
           M+ + L + +F   + + ++ L        NLPPSP    P IGHLHL+K P+HRR   L
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 61  SEKYGH--VFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVV 120
           SE   +  +FSL  GSRLV VVSS +   ECFTKND+VLANRP    GKH+ YN T +V 
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 121 APYGEYWRNLRRISTLEIFSASRLNLF---------------SGSDKMKFYVVELEPMFS 180
           A YG+ WRNLRRI T+EIFS+ RLN F               + + +  F  VE++P+F 
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187

Query: 181 DLMSNIVMRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTG 240
            L  N ++RMVAGKR+YGD   ++ +AK  R+++  V++  GA N  D+ PI  ++  T 
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TN 247

Query: 241 LEKKI----------------------IKG------------TQPEHFSDQIIKGLIHVI 295
            EK +                      +KG            TQP++++D IIKG+I V+
Sbjct: 248 YEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 307

[Close]

BLAST of Sgr021313 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 249.2 bits (635), Expect = 4.1e-66
Identity = 139/340 (40.88%), Postives = 202/340 (59.41%), Query Frame = 0


Query: 5   LLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYGH- 64
           L++  +F++  L+ +   L+   NLPPSP +SLP IGHL L+K PIHR    LS+   + 
Sbjct: 6   LIFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNA 65

Query: 65  -VFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEYW 124
            +FSL  G+RLV V SS S   ECFTKND+VLANRP     KH+ Y++T ++ A YG++W
Sbjct: 66  PIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHW 125

Query: 125 RNLRRISTLEIFSASRLNLF---------------SGSDKMKFYVVELEPMFSDLMSNIV 184
           RNLRRI ++EIFS  RLN F               S +   +F  V+++ M SDL  N +
Sbjct: 126 RNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNI 185

Query: 185 MRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKIIK 244
           +RMVAGKRYYGD + D+ +AK+ R+++  V+  AGA N  D++P+   +       K + 
Sbjct: 186 LRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVSDYETRVKKLA 245

Query: 245 G--------------------------------TQPEHFSDQIIKGLIHVILLAGIDTAA 296
           G                                +QP++F+D+IIKG +  ++LAG DT+A
Sbjct: 246 GRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSA 305

[Close]

BLAST of Sgr021313 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 248.8 bits (634), Expect = 5.4e-66
Identity = 145/343 (42.27%), Postives = 198/343 (57.73%), Query Frame = 0


Query: 5   LLYVCVFLISLLVVVRCLLRSGRNLPPSPRFSLPFIGHLHLIKHPIHRRLHELSEKYGH- 64
           L++  +F+   L  +   ++   NLPPSP ++LP IGHL L+K P+HR    +SE  G  
Sbjct: 6   LIFTFLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDA 65

Query: 65  -VFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLVVAPYGEYW 124
            + SL  G+RLV VVSS S   ECFTKND+VLANR      KH++Y  T +V A YG++W
Sbjct: 66  PIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHW 125

Query: 125 RNLRRISTLEIFSASRLNLFSG---------------SDKMKFYVVELEPMFSDLMSNIV 184
           RNLRRI  +EIFSA RLN FS                +  ++F  VE++ MFS+L  N +
Sbjct: 126 RNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNI 185

Query: 185 MRMVAGKRYYGDDMLDEEKAKQFREIVKRVMLHAGATNPGDFIPIWNWIDPTGLEKKI-- 244
           +RM+AGK YYGD   D+ +AK+ RE++   M   GA N  D++PI  WI  TG EK+I  
Sbjct: 186 IRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI--TGSEKRIKK 245

Query: 245 ---------------------------------IKGTQPEHFSDQIIKGLIHVILLAGID 296
                                            ++ TQPE+++D IIKG++  ++LAG D
Sbjct: 246 IASRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTD 305

The following BLAST results are available for this feature:

BLAST of Sgr021313 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901746.1	1.0e-122	66.86	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
XP_011650030.1	6.2e-117	64.64	cytochrome P450 81Q32 [Cucumis sativus] >KGN63296.1 hypothetical protein Csa_022...	[more]
XP_023512110.1	2.0e-115	65.40	cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022985606.1	2.0e-115	65.40	cytochrome P450 81D1-like [Cucurbita maxima]	[more]
XP_022943756.1	2.2e-114	64.81	cytochrome P450 81D1-like [Cucurbita moschata]	[more]

BLAST of Sgr021313 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	1.7e-80	47.62	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ9	2.9e-77	44.87	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q6WNQ8	2.8e-75	46.20	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
P93147	2.2e-72	43.44	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNR0	1.6e-70	42.77	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of Sgr021313 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTD0	3.0e-117	64.64	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1	[more]
A0A6J1J8Q1	9.7e-116	65.40	cytochrome P450 81D1-like OS=Cucurbita maxima OX=3661 GN=LOC111483619 PE=3 SV=1	[more]
A0A6J1FSK8	1.1e-114	64.81	cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448412 PE=3 SV=...	[more]
A0A6J1D5S1	6.5e-104	59.71	cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV...	[more]
A0A6J1D5S6	4.0e-101	57.68	isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE...	[more]

BLAST of Sgr021313 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37320.1	1.8e-69	41.74	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT4G37400.1	5.7e-68	41.00	cytochrome P450, family 81, subfamily F, polypeptide 3	[more]
AT3G28740.1	8.3e-67	41.67	Cytochrome P450 superfamily protein	[more]
AT4G37370.1	4.1e-66	40.88	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37340.1	5.4e-66	42.27	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 58..77 score: 43.9 coord: 82..103 score: 22.41 coord: 162..180 score: 25.97
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 226..295 e-value: 4.6E-13 score: 50.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..225 e-value: 2.5E-35 score: 123.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 30..294
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 30..225 e-value: 3.2E-18 score: 65.7 coord: 230..294 e-value: 5.1E-11 score: 42.0
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 228..295 coord: 7..227
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 228..295
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 7..227

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr021313.1	Sgr021313.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen