Sgr021250 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr021250
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	S-norcoclaurine synthase 1-like
Location	tig00153654: 101501 .. 103665 (+)
RNA-Seq Expression	Sgr021250
Synteny	Sgr021250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCGGTCAACTCAGGCAAGAGACGGCGATCCAGGCGCCGGCCGCCGTAGCATGGAAGCTCTACAGCGGCCTTGAACTGGGAAGACTCGTCGAGAAGCAGCTCTCGAATCTCATCCACAAAGTTGAGGTCGTTGAAGGCGACGGAGGAGAAGGAACTGTTCTTAATCTCATACTCCCACCAGGTAAAAAAATCGTATCATCTCTTTCTCTGGTTTCCTACTTTCTTTCCTCTGTAAATTTTCTGGGAAAATGTTTGAAGATTTTCTCGGAAAAAGCTTGAGAGACCTCTAAATTTCTGTGCACTTTTACACCATATCCTTCAATTTCCTTTGTTTGTGCGTACTAAAATGTTAACGATCGAGATATTTTCTTTTGGGTTTCACTTGGGAATGAATTTTAGTGAATTTTTATAGAAAGGACTTGATCTGATTTAGGGGTTATGATAGGTTAATAGAGGTGATAAAAAAAATTCGATGATCTGATAAATGTAATCCAAATTATATGGTTTGAGTTGGATTATAAGTTCAGCATATATGCCCAATAGCCACTTCTGAGAGTAAAATTTAGTTTAAACCCGATATTGAAAATTATTTCGAGAACCACCAAGTCCATGTCAATCTTCTCTTCTTTGTATTTTATTAATATCTCATTCATATAAATCGAAACTAACCTTTCATTTCTCAAATAATTAAATATGTATATAATACAAACACACGTATTTATAATTTTTAAGCTTTTAAAGTATTAAATTTTGTAGTTATTTATAGAAAAAAATCAATTTTTACCCTGAACTTAGCAAGGTGTGTCAATTTTTATCTCTTAAACTTAGATAAGTGTTGCAATCTTTACCTTAAACTTTATTAAGTGTCGCAATTTTTATCTTCTGACAATTTTTCGTTAGACGAATCTTTAAAAAATCAATTTTTACCCTGAACTTAGCAAGATGTGTCAATTTTTATCTCTTAAATTTAGATAAGTGTTGCAATCGTTACCTCAAATTTTATTAAGTGTCACAATTTTTATTGAAATTAATGAGTTTTTGGAGACTTTTACCCTGCTTTTTATAAAAATTAGTGTGTGAAATAATGAAAATTGAATCGAGGGTAAAAATTATAACACTTATCTAAATCTAGGGTCCAATTTGATGAAATTGAAAGTTTATGATGAACATTGATACATCTTAGTAAGTTCATGGGTAAAATATGATTTTTTTCCCTTTATTTATATATATAGGTTGATAATTATTGAAATTTTTTCATTTTTTACCCATATACAGCTCATGAAGTCAATTTGATTCTCAAACTTTTAATCTCATCAAATTCTTTATACTGAATAAGTGGTGACATTAATACCCTTAATTAATAAAGTTTTCTTTTTCTTTGATTAATACCCAAAAACAGAATATGAATGGCTATTCTCTAAGATCATTTATTAACATATTTACGTTCTCTTCTTGATGTGTCAAGTGATCCAAATAAGAAGTCATCTCCAAACTTTTTTCACCCACGATCTGTTTGCATAAACAAGGAAAAAAGACAAATGAAAAGTTGCTTTTCACTCAACTTGGCATGTACATTTTATAATGGAAAGTGCTGGCAAAGAAATTAATTTGACATGACCTTTAGATGATCCCATTGAACCAAGAATATAATGTGCTGACAATAGATTCTAATTGTGCTATAAAATCCATTGTCAACCTAAGTAACTTGTAATATAATTAATTTTTAAAAACAAACCCCAACCAGCTAGCATCACCTAAGACACGTGACGTCTTAAATTTGATAGAGTAATTTAGAGCAGCTCATGTCATTATAATTTAATTGAATCTAATGCAAGAGTAATGAAAAAATTTACACCTATACAGGTTCAGGCAGTGCCTCAAGTTACAAAGAGAAATTCACAAAAATAGATAATGAGAATCGTATAAAAGAAACAGAGGTGGTGGAAGGAGGGTACTTGAATCTCGGCTTTACTCTTTATAGGATTCGTTTAAAGATAATTGAGAAGGATGATGATAGCTGCATTGTTGAATCCACAATTGAATATGAGGTGAAGGAAGAAGCTGCTGCAAATATCTCACTTGTAAGCATACAACCTCTTATAGACATTGCTCAAGCTGCAAACAACCATCTCCTCAAGAACCAGCAGGCTAAAGATGTATAA

mRNA sequence

ATGTTCGGTCAACTCAGGCAAGAGACGGCGATCCAGGCGCCGGCCGCCGTAGCATGGAAGCTCTACAGCGGCCTTGAACTGGGAAGACTCGTCGAGAAGCAGCTCTCGAATCTCATCCACAAAGTTGAGGTCGTTGAAGGCGACGGAGGAGAAGGAACTGTTCTTAATCTCATACTCCCACCAGGTTCAGGCAGTGCCTCAAGTTACAAAGAGAAATTCACAAAAATAGATAATGAGAATCGTATAAAAGAAACAGAGGTGGTGGAAGGAGGGTACTTGAATCTCGGCTTTACTCTTTATAGGATTCGTTTAAAGATAATTGAGAAGGATGATGATAGCTGCATTGTTGAATCCACAATTGAATATGAGGTGAAGGAAGAAGCTGCTGCAAATATCTCACTTGTAAGCATACAACCTCTTATAGACATTGCTCAAGCTGCAAACAACCATCTCCTCAAGAACCAGCAGGCTAAAGATGTATAA

Coding sequence (CDS)

ATGTTCGGTCAACTCAGGCAAGAGACGGCGATCCAGGCGCCGGCCGCCGTAGCATGGAAGCTCTACAGCGGCCTTGAACTGGGAAGACTCGTCGAGAAGCAGCTCTCGAATCTCATCCACAAAGTTGAGGTCGTTGAAGGCGACGGAGGAGAAGGAACTGTTCTTAATCTCATACTCCCACCAGGTTCAGGCAGTGCCTCAAGTTACAAAGAGAAATTCACAAAAATAGATAATGAGAATCGTATAAAAGAAACAGAGGTGGTGGAAGGAGGGTACTTGAATCTCGGCTTTACTCTTTATAGGATTCGTTTAAAGATAATTGAGAAGGATGATGATAGCTGCATTGTTGAATCCACAATTGAATATGAGGTGAAGGAAGAAGCTGCTGCAAATATCTCACTTGTAAGCATACAACCTCTTATAGACATTGCTCAAGCTGCAAACAACCATCTCCTCAAGAACCAGCAGGCTAAAGATGTATAA

Protein sequence

MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILPPGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTIEYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQAKDV

Homology

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BLAST of Sgr021250 vs. NCBI nr
Match: XP_022986627.1 (S-norcoclaurine synthase 2-like [Cucurbita maxima])

HSP 1 Score: 256.1 bits (653), Expect = 1.9e-64
Identity = 128/159 (80.50%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           M GQL  E AIQAPA VAW+LY GLEL RLVE +LSNLI K+EVVEGDGGEGTVLNLI P
Sbjct: 1   MLGQLSHEAAIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           PG G ASSYKEKFTKIDNENRIKETEVVEGG+L++GFTLYR+RLKI+E  DDSCIVESTI
Sbjct: 61  PGLGGASSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQAKD 160
           EYE+KEEAAAN SLV++QPLIDIAQAAN+HLL  +Q KD
Sbjct: 121 EYEIKEEAAANASLVTLQPLIDIAQAANDHLLHYKQLKD 159

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BLAST of Sgr021250 vs. NCBI nr
Match: XP_022943440.1 (S-norcoclaurine synthase 1-like [Cucurbita moschata])

HSP 1 Score: 252.7 bits (644), Expect = 2.2e-63
Identity = 126/159 (79.25%), Postives = 139/159 (87.42%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           M GQL  E  IQAPA VAW+LY GLEL RLVE +LSNLI K+EVVEGDGGEGTVLNLI P
Sbjct: 1   MLGQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           PG G A SYKEKFTKIDNENRIKETEVVEGG+L++GFTLYR+RLKI+E  DDSCIVESTI
Sbjct: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQAKD 160
           EYE+KEEAAAN SLV++QPLIDIAQAAN+HLL  +Q KD
Sbjct: 121 EYEIKEEAAANASLVTLQPLIDIAQAANDHLLHYKQLKD 159

[Close]

BLAST of Sgr021250 vs. NCBI nr
Match: XP_022148581.1 (S-norcoclaurine synthase 2-like [Momordica charantia])

HSP 1 Score: 248.8 bits (634), Expect = 3.1e-62
Identity = 128/157 (81.53%), Postives = 143/157 (91.08%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           MFG+LR E+AIQAPAAV W+LY GLELGRLVE +L NLI KVEVVEGDGGEGTVLNLI P
Sbjct: 1   MFGELRHESAIQAPAAVVWQLYGGLELGRLVENRLWNLIQKVEVVEGDGGEGTVLNLIFP 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           PG G A+SYKEKFTKIDNE RIKETE+VEGG+L+LGFTLYR+RLKIIEKDDD+ IVESTI
Sbjct: 61  PGLG-ATSYKEKFTKIDNEKRIKETEIVEGGFLDLGFTLYRVRLKIIEKDDDTSIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQA 158
           EYE+KEEAAAN SLV+IQPLIDIA+AAN+HLL  Q+A
Sbjct: 121 EYELKEEAAANASLVTIQPLIDIAEAANSHLLDIQKA 156

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BLAST of Sgr021250 vs. NCBI nr
Match: XP_023513283.1 (S-norcoclaurine synthase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 248.4 bits (633), Expect = 4.1e-62
Identity = 125/159 (78.62%), Postives = 139/159 (87.42%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           M GQL  E AIQAPA VAW+LY GLEL RLVE +LSNLI K+EVVEG+GGEGTVLNLI  
Sbjct: 1   MLGQLSHEAAIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGNGGEGTVLNLIFL 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           PG G A SYKEKFTKIDNENRIKETEVVEGG+L++GFTLYR+RLKI+E  DDSCIVESTI
Sbjct: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQAKD 160
           EYE+KEEAAAN SLV++QPLIDIAQAAN+HLL  +Q KD
Sbjct: 121 EYEIKEEAAANASLVTLQPLIDIAQAANDHLLHYKQLKD 159

[Close]

BLAST of Sgr021250 vs. NCBI nr
Match: XP_023522409.1 (S-norcoclaurine synthase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 247.3 bits (630), Expect = 9.0e-62
Identity = 122/160 (76.25%), Postives = 138/160 (86.25%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           M GQL  E AIQAPA V W+LY GLEL RL+E +L NLI K+EVVEGDGGEGTVLN+I P
Sbjct: 1   MLGQLSHEAAIQAPATVVWQLYGGLELARLIENRLPNLIKKIEVVEGDGGEGTVLNIIFP 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           PG G A  YKEKFTKIDNENRIKETEVVEGG+L++GFTLYR+RLKI+E  DDSCIVESTI
Sbjct: 61  PGLGGAPGYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQAKDV 161
           EY++KEE AAN SLV+IQPLIDIAQAAN+HLL N+Q K+V
Sbjct: 121 EYDIKEEDAANASLVTIQPLIDIAQAANDHLLHNKQHKNV 160

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BLAST of Sgr021250 vs. ExPASy Swiss-Prot
Match: A0A3G5BB24 (Norbelladine synthase OS=Narcissus pseudonarcissus OX=39639 GN=NBS PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.1e-30
Identity = 71/155 (45.81%), Postives = 98/155 (63.23%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           M G L  E  +  PA   W++YS L L +L  + L  +I KVEV EGDGG GT+L +   
Sbjct: 1   MKGSLSHELEVSLPADQLWQVYSTLRLAQLSAELLPTVISKVEVEEGDGGVGTLLRVTYA 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
            G      +KE+F KID+E R+KE   VEGG+L+LGF+ Y IRL+I+EK  +S +++ST+
Sbjct: 61  LGIPGMKYHKERFVKIDHEKRLKEALFVEGGHLDLGFSSYLIRLEILEKGHNSSVIKSTV 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQ 156
           EYEV EE AAN S  +  P + I  A + HLL+ +
Sbjct: 121 EYEVDEEHAANASFATTDPFMIIGGAVSEHLLQKK 155

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BLAST of Sgr021250 vs. ExPASy Swiss-Prot
Match: Q4QTJ1 (S-norcoclaurine synthase 2 OS=Papaver somniferum OX=3469 GN=NCS2 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 9.8e-27
Identity = 62/154 (40.26%), Postives = 103/154 (66.88%), Query Frame = 0


Query: 4   QLRQETAIQAPAAVAWKLYSGLELGRLV-EKQLSNLIHKVEVVEGDGGEGTVLNLILPPG 63
           ++R +  +   A   W +YS  ++ RL+ +  L  +  K++V+EG+GG GTVL+++ PPG
Sbjct: 42  EIRYDLEVPTSADSIWSVYSCPDIPRLLRDVLLPGVFQKLDVIEGNGGVGTVLDIVFPPG 101

Query: 64  SGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTIEY 123
           +    SYKEKF  I++E R+KE  ++EGGYL++G T Y  R+ I EK  +SC++ES+I Y
Sbjct: 102 A-VPRSYKEKFVNINHEKRLKEVIMIEGGYLDMGCTFYMDRIHIFEKTPNSCVIESSIIY 161

Query: 124 EVKEEAAANIS-LVSIQPLIDIAQAANNHLLKNQ 156
           EVKEE A  ++ L++ +PL  +A+  + ++LK +
Sbjct: 162 EVKEEYAGKMAKLITTEPLESMAEVISGYVLKKR 194

[Close]

BLAST of Sgr021250 vs. ExPASy Swiss-Prot
Match: Q4QTJ2 (S-norcoclaurine synthase 1 OS=Papaver somniferum OX=3469 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 6.4e-26
Identity = 62/154 (40.26%), Postives = 101/154 (65.58%), Query Frame = 0


Query: 4   QLRQETAIQAPAAVAWKLYSGLELGRLV-EKQLSNLIHKVEVVEGDGGEGTVLNLILPPG 63
           ++  ET +Q  A   W +YS  ++ RL+ +  L  +  K++V+ G+GG GTVL++  P G
Sbjct: 42  EITYETEVQTSADSIWNVYSSPDIPRLLRDVLLPGVFEKLDVIAGNGGVGTVLDIAFPLG 101

Query: 64  SGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTIEY 123
           +     YKEKF KI++E R+KE  ++EGGYL++G T Y  R+ I EK  +SC++ES+I Y
Sbjct: 102 A-VPRRYKEKFVKINHEKRLKEVVMIEGGYLDMGCTFYMDRIHIFEKTPNSCVIESSIIY 161

Query: 124 EVKEEAAANIS-LVSIQPLIDIAQAANNHLLKNQ 156
           EVKEE A  ++ L++ +PL  +A+  + ++LK +
Sbjct: 162 EVKEEYAGKMAKLITTEPLESMAEVISGYVLKKR 194

[Close]

BLAST of Sgr021250 vs. ExPASy Swiss-Prot
Match: Q67A25 (S-norcoclaurine synthase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.2e-19
Identity = 53/152 (34.87%), Postives = 88/152 (57.89%), Query Frame = 0


Query: 5   LRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILPPGSG 64
           +  E  + A A   W +YS   L + +   L     K+E++ GDGG GT+L++   PG  
Sbjct: 46  IHHELEVAASADDIWTVYSWPGLAKHLPDLLPGAFEKLEII-GDGGVGTILDMTFVPGE- 105

Query: 65  SASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTIEYEV 124
               YKEKF  +DNE+R+K+ +++EGGYL+LG T Y   + ++    DSC+++S+ EY V
Sbjct: 106 FPHEYKEKFILVDNEHRLKKVQMIEGGYLDLGVTYYMDTIHVVPTGKDSCVIKSSTEYHV 165

Query: 125 KEEAAANIS-LVSIQPLIDIAQAANNHLLKNQ 156
           K E    +  L++  PL  +A A +  +L+++
Sbjct: 166 KPEFVKIVEPLITTGPLAAMADAISKLVLEHK 195

[Close]

BLAST of Sgr021250 vs. ExPASy Swiss-Prot
Match: A2A1A1 (S-norcoclaurine synthase 2 OS=Coptis japonica OX=3442 GN=PR10A PE=2 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 1.2e-16
Identity = 51/152 (33.55%), Postives = 81/152 (53.29%), Query Frame = 0


Query: 5   LRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILPPGSG 64
           L  E  + A A   W +    ELG  +   L   I     + GDGGEG++L++  PPG  
Sbjct: 41  LYHELEVAASADEVWSVEGSPELGLHLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQ- 100

Query: 65  SASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTIEYEV 124
               Y+EKF   D++NR K  E ++G + +LG T Y   ++++    DSC+++ST EY V
Sbjct: 101 FPHHYREKFVFFDHKNRYKLVEQIDGDFFDLGVTYYMDTIRVVATGPDSCVIKSTTEYHV 160

Query: 125 KEEAAANIS-LVSIQPLIDIAQAANNHLLKNQ 156
           K E A  +  L+   PL  +++A    +L+N+
Sbjct: 161 KPEFAKIVKPLIDTVPLAIMSEAIAKVVLENK 191

[Close]

BLAST of Sgr021250 vs. ExPASy TrEMBL
Match: A0A6J1J835 (S-norcoclaurine synthase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484311 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 9.4e-65
Identity = 128/159 (80.50%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           M GQL  E AIQAPA VAW+LY GLEL RLVE +LSNLI K+EVVEGDGGEGTVLNLI P
Sbjct: 1   MLGQLSHEAAIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           PG G ASSYKEKFTKIDNENRIKETEVVEGG+L++GFTLYR+RLKI+E  DDSCIVESTI
Sbjct: 61  PGLGGASSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQAKD 160
           EYE+KEEAAAN SLV++QPLIDIAQAAN+HLL  +Q KD
Sbjct: 121 EYEIKEEAAANASLVTLQPLIDIAQAANDHLLHYKQLKD 159

[Close]

BLAST of Sgr021250 vs. ExPASy TrEMBL
Match: A0A6J1FXT6 (S-norcoclaurine synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111448206 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.0e-63
Identity = 126/159 (79.25%), Postives = 139/159 (87.42%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           M GQL  E  IQAPA VAW+LY GLEL RLVE +LSNLI K+EVVEGDGGEGTVLNLI P
Sbjct: 1   MLGQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           PG G A SYKEKFTKIDNENRIKETEVVEGG+L++GFTLYR+RLKI+E  DDSCIVESTI
Sbjct: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQAKD 160
           EYE+KEEAAAN SLV++QPLIDIAQAAN+HLL  +Q KD
Sbjct: 121 EYEIKEEAAANASLVTLQPLIDIAQAANDHLLHYKQLKD 159

[Close]

BLAST of Sgr021250 vs. ExPASy TrEMBL
Match: A0A6J1D5U1 (S-norcoclaurine synthase 2-like OS=Momordica charantia OX=3673 GN=LOC111017212 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.5e-62
Identity = 128/157 (81.53%), Postives = 143/157 (91.08%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           MFG+LR E+AIQAPAAV W+LY GLELGRLVE +L NLI KVEVVEGDGGEGTVLNLI P
Sbjct: 1   MFGELRHESAIQAPAAVVWQLYGGLELGRLVENRLWNLIQKVEVVEGDGGEGTVLNLIFP 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           PG G A+SYKEKFTKIDNE RIKETE+VEGG+L+LGFTLYR+RLKIIEKDDD+ IVESTI
Sbjct: 61  PGLG-ATSYKEKFTKIDNEKRIKETEIVEGGFLDLGFTLYRVRLKIIEKDDDTSIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQA 158
           EYE+KEEAAAN SLV+IQPLIDIA+AAN+HLL  Q+A
Sbjct: 121 EYELKEEAAANASLVTIQPLIDIAEAANSHLLDIQKA 156

[Close]

BLAST of Sgr021250 vs. ExPASy TrEMBL
Match: A0A6J1FUB4 (S-norcoclaurine synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111448205 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.3e-60
Identity = 121/160 (75.62%), Postives = 135/160 (84.38%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           M GQL  E AIQA A V W+LY GLEL RLVE +L NLI K+EVVEGDGGEGTVLN+I  
Sbjct: 1   MLGQLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEGDGGEGTVLNIIFQ 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           P  G A  YKEKFTKIDNENRIKETEVVEGG+L++GFTLYR+RLKI+E  DDSCIVESTI
Sbjct: 61  PSLGGAPGYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQAKDV 161
           EY++KEE AAN SLVSIQPLIDIAQAAN+HLL N+Q K+V
Sbjct: 121 EYDIKEEDAANASLVSIQPLIDIAQAANDHLLHNKQHKNV 160

[Close]

BLAST of Sgr021250 vs. ExPASy TrEMBL
Match: A0A6J1FY33 (S-norcoclaurine synthase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111448917 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.0e-59
Identity = 118/159 (74.21%), Postives = 135/159 (84.91%), Query Frame = 0


Query: 1   MFGQLRQETAIQAPAAVAWKLYSGLELGRLVEKQLSNLIHKVEVVEGDGGEGTVLNLILP 60
           M G+L  E+ IQAPA VAW+LY G EL RLVE + SNLI K+EVVEGDGGEGTVLNLI P
Sbjct: 1   MLGKLSHESVIQAPATVAWQLYGGFELARLVENRFSNLIQKIEVVEGDGGEGTVLNLIFP 60

Query: 61  PGSGSASSYKEKFTKIDNENRIKETEVVEGGYLNLGFTLYRIRLKIIEKDDDSCIVESTI 120
           PG G  SS+KEKFT+IDNENRIKETEVVEGG+L++GFTLYR+RLK++E  DDSCIVESTI
Sbjct: 61  PGVGRFSSFKEKFTRIDNENRIKETEVVEGGFLDIGFTLYRVRLKMVENGDDSCIVESTI 120

Query: 121 EYEVKEEAAANISLVSIQPLIDIAQAANNHLLKNQQAKD 160
           EYE+KEEAAAN SLV+IQ  IDIAQ  N+HLL N+Q KD
Sbjct: 121 EYEIKEEAAANASLVNIQFFIDIAQTENDHLLHNKQPKD 159

The following BLAST results are available for this feature:

BLAST of Sgr021250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986627.1	1.9e-64	80.50	S-norcoclaurine synthase 2-like [Cucurbita maxima]	[more]
XP_022943440.1	2.2e-63	79.25	S-norcoclaurine synthase 1-like [Cucurbita moschata]	[more]
XP_022148581.1	3.1e-62	81.53	S-norcoclaurine synthase 2-like [Momordica charantia]	[more]
XP_023513283.1	4.1e-62	78.62	S-norcoclaurine synthase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023522409.1	9.0e-62	76.25	S-norcoclaurine synthase 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sgr021250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A3G5BB24	1.1e-30	45.81	Norbelladine synthase OS=Narcissus pseudonarcissus OX=39639 GN=NBS PE=1 SV=1	[more]
Q4QTJ1	9.8e-27	40.26	S-norcoclaurine synthase 2 OS=Papaver somniferum OX=3469 GN=NCS2 PE=1 SV=1	[more]
Q4QTJ2	6.4e-26	40.26	S-norcoclaurine synthase 1 OS=Papaver somniferum OX=3469 GN=NCS1 PE=1 SV=1	[more]
Q67A25	1.2e-19	34.87	S-norcoclaurine synthase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1	[more]
A2A1A1	1.2e-16	33.55	S-norcoclaurine synthase 2 OS=Coptis japonica OX=3442 GN=PR10A PE=2 SV=2	[more]

BLAST of Sgr021250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J835	9.4e-65	80.50	S-norcoclaurine synthase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484311 PE=3...	[more]
A0A6J1FXT6	1.0e-63	79.25	S-norcoclaurine synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111448206 PE...	[more]
A0A6J1D5U1	1.5e-62	81.53	S-norcoclaurine synthase 2-like OS=Momordica charantia OX=3673 GN=LOC111017212 P...	[more]
A0A6J1FUB4	5.3e-60	75.63	S-norcoclaurine synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111448205 PE...	[more]
A0A6J1FY33	2.0e-59	74.21	S-norcoclaurine synthase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111448917 PE...	[more]

BLAST of Sgr021250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 3..154 e-value: 1.5E-15 score: 57.4
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 1..156 e-value: 8.5E-42 score: 144.7
None	No IPR available	PANTHER	PTHR31213:SF145	S-NORCOCLAURINE SYNTHASE 2-LIKE	coord: 3..156
None	No IPR available	PANTHER	PTHR31213	FAMILY NOT NAMED	coord: 3..156
None	No IPR available	CDD	cd07816	Bet_v1-like	coord: 3..128 e-value: 6.16034E-35 score: 117.29
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 4..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr021250.1	Sgr021250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009738	abscisic acid-activated signaling pathway
biological_process	GO:0006952	defense response
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0080163	regulation of protein serine/threonine phosphatase activity
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0010427	abscisic acid binding
molecular_function	GO:0004864	protein phosphatase inhibitor activity
molecular_function	GO:0038023	signaling receptor activity