Sgr021221 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021221
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionAB hydrolase-1 domain-containing protein
Locationtig00153653: 219472 .. 222328 (+)
RNA-Seq ExpressionSgr021221
SyntenySgr021221
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGTATTTGAATCTCCTTCTCCTCTTCTTCTTCTTCTTCCTTTCTTCTACAGTTGCTAAAACGCGATCAGATCACCCGCCGCCGGGAGGTGGTGGTGGTAAGCACTTCGTTCTGGTTCACGGGGCCTGCCATGGCGCTTGGTCGTGGTACAAGCTGTCGACGATGCTGCGGTTGGCGGGCCACAGCGTGACGGCGCTGGACATGGCGGCTGCCGGAATTGATCGGAGGGAGGCGGAGAGTCTGAGATCGTTCTCTGAATACGTCGAGCCATTGACGGATTTCATGGCGGAGTTGGCGGCGGAGGAGAAGGTGATCCTCGTAGGGCATAGCCAAGGGGGGCTGTGCATCTCCAAAGCCATGGAGGATTTTCCAGATAAAGTTTCTGTGGCTGTTTTCGTCTCTGCCGCCATGCCTGGCCCATTCCTTAATGCTTCTTTTCTTCTTCACGAGGTATTAATCTCTTAATTTTACATAAAATAGGTTAAATTATTTTCTATGGATTTTTATCATTCCTGACGAAATTTCAATTTCGTCTCTGTCATTTAATAAAATCTTATAAATTATCTAGATAAGCAAGCAAATATATAAAAACGAAACCACCTTCAAATTGAACTACAGACAAATAGAAGTTTTTTTTTAAGCTTGCTCATAGGAAATGAAAATTTGTCTGTTAGCTCAGCTCCCTCTACTGGTTGATGTTTGGTTGTGTTAATTGATTGTATTAAAATTTGTGAAGAGTAAAAAAAAAAAGAAGTAAAGTAGGTAAAAAAGAAGTATGTTTCCAAGTAGATAAATGACGGTTTATGGATAATTAATGAGATTTAACCAATCAAAACTAAGCTCAAATATAAAGACTAAATGGAAACTTAAAAAAAAAAAAATCAAATTAGTAATTTTACTTATACAAAATTATTTAAATACATATGTAGTGTCTTTAATTTTTTAAAAGTAATTATTTAATAGAATTTGAAATTTATTTTAAAATCACTTGATATGACATTCGACAAATTTTGACTAAATTAACTCATAATCAACTGAATAGTAATAATCGAAAAAAAAAATCTAGTACTATTGTAGTACCTACTCTTCCATTTATATTTAAACACATAATCATTAATGTTCTAATATTTTCATAGATCCGATACTATTGTATAATGAAAAATTTTCCCATAATGACCATGCCTAGTAAGCATTTCTTCACTGTAGGAATTAGATAAAAATAGAATTTAAAATTTAAAAATATAATTGAAATTTATTAAAATTGAAGAATTAAAGAGATATAAGGCTCGAAATACAGGAATATAGAGTCGATTCAAGGCAAGAAATGTATTTTATTTATTTTTATTCTTGAGGTGTCGAGAAAAAAAAGTTATTTGATCTCTGAAATAGAGATTATAGAATATGTGTTGAGAATTTATCTTATTGTTTACATTAATATACCATTTATAAATATTTACAAGAATTGGGATGCAATAACACAAGGAAGAAGGAATGGAAAATTTAAAATCAAATTAAATGAATATATTAATAAATCTAATAGATTTTAAATTAAAAAAAATTAAAAAGTTTGTGATTTATTTAGATAAAGAAATCTAAAATAAATTAAAATAGCAAAGTTCAAAAAATTAAATATTGCAAATCATTATTCAAATTAAATTAGTAGTAGTTAGAGAAAAAAATGATAAAATAATAAAAATATATATAATCATGAATATTTAAAAATGAATGATATATTTGTAAATATATATATATTTTTTCATTGAATGATATATTATTGACCATTTGTCTTTTCTCACAGCTTCGCAAGAGGATAGATTTTGGGCCAGACACCCGTTATACATATGGTAATGGGCCCAAAAACCCTCCGACAACTTTGACATTCGGCCCACTGTTTTCAGCATCAAAACTATACCAAAACAGCCCCAAAGAGGTAAATTAATTACCTATCTAAATAAGTAATATATGTATGTATGTGTATTACAATTTGGATCTAACAAAAGGTATTTATTGGTTAACAAATTTTACTCAATAACACTTTTTCGAGAATTAATTAGTAGTATTGAATAATTATTGAATTATATTCAAATAACAATTTCTCTCTGATTTTTTTTATAGAATAATAATTATTTAAACGTATTGTTGGTAATACATATAAAATTACACAAACCAAAAACTTCCAAGTTCGCAAGTTTTACAAACCCAAGTTTATTAAAACTGCAAGCCAAATATTTCCTAACAGAATCAATACATATGATATAACTTATTATTTACATGTGTCGTTAAAGATAGAAGATAGAAATATATATGATTTGTAAAAGCTCAGAAGCTATATACATGTTTGAAATCACCTTCAAATACATGGATATAATTTGTAAAATTAACAAACTATGATGAGATATTTATCACGTGATTTTTAACAAGTTTGGATTTGTTAAGCCTATGTATATGTATTTATAACATGGGATAGTTCAATTTGGCTCAATTTTCTTATTCTAATTTACACAAACCTAACCCATGACTTAAATTCGAGAAGATTCTCTAATTATTGGCAATTGGTTCATTGTTGAAGGACTTGACACTGGCCAACACGTTAATGAGACCAACCCATTTGTTTGAGGCACAACAATGGAGCAAGGATCTACATTTGACGACAGAGAGGTATGGCGCCGTCAAACGAGTTTTCGTGGTTTCAGGGAAGGATATGGTGATCAAAAAGAGCTTCCAACATTGGGTGATCAAAAGAAATCCACCTACTAATGTGTTGGAAGTTGGAGGATCGGATCATATGGTGATGATGTCCAAGCCGTTCGATCTCTTCAAGAAACTCAACCGCATTGCTCGACACTATTCTTAG

mRNA sequence

ATGACGTATTTGAATCTCCTTCTCCTCTTCTTCTTCTTCTTCCTTTCTTCTACAGTTGCTAAAACGCGATCAGATCACCCGCCGCCGGGAGGTGGTGGTGGTAAGCACTTCGTTCTGGTTCACGGGGCCTGCCATGGCGCTTGGTCGTGGTACAAGCTGTCGACGATGCTGCGGTTGGCGGGCCACAGCGTGACGGCGCTGGACATGGCGGCTGCCGGAATTGATCGGAGGGAGGCGGAGAGTCTGAGATCGTTCTCTGAATACGTCGAGCCATTGACGGATTTCATGGCGGAGTTGGCGGCGGAGGAGAAGGTGATCCTCGTAGGGCATAGCCAAGGGGGGCTGTGCATCTCCAAAGCCATGGAGGATTTTCCAGATAAAGTTTCTGTGGCTGTTTTCGTCTCTGCCGCCATGCCTGGCCCATTCCTTAATGCTTCTTTTCTTCTTCACGAGCTTCGCAAGAGGATAGATTTTGGGCCAGACACCCGTTATACATATGGTAATGGGCCCAAAAACCCTCCGACAACTTTGACATTCGGCCCACTGTTTTCAGCATCAAAACTATACCAAAACAGCCCCAAAGAGGACTTGACACTGGCCAACACGTTAATGAGACCAACCCATTTGTTTGAGGCACAACAATGGAGCAAGGATCTACATTTGACGACAGAGAGGTATGGCGCCGTCAAACGAGTTTTCGTGGTTTCAGGGAAGGATATGGTGATCAAAAAGAGCTTCCAACATTGGGTGATCAAAAGAAATCCACCTACTAATGTGTTGGAAGTTGGAGGATCGGATCATATGGTGATGATGTCCAAGCCGTTCGATCTCTTCAAGAAACTCAACCGCATTGCTCGACACTATTCTTAG

Coding sequence (CDS)

ATGACGTATTTGAATCTCCTTCTCCTCTTCTTCTTCTTCTTCCTTTCTTCTACAGTTGCTAAAACGCGATCAGATCACCCGCCGCCGGGAGGTGGTGGTGGTAAGCACTTCGTTCTGGTTCACGGGGCCTGCCATGGCGCTTGGTCGTGGTACAAGCTGTCGACGATGCTGCGGTTGGCGGGCCACAGCGTGACGGCGCTGGACATGGCGGCTGCCGGAATTGATCGGAGGGAGGCGGAGAGTCTGAGATCGTTCTCTGAATACGTCGAGCCATTGACGGATTTCATGGCGGAGTTGGCGGCGGAGGAGAAGGTGATCCTCGTAGGGCATAGCCAAGGGGGGCTGTGCATCTCCAAAGCCATGGAGGATTTTCCAGATAAAGTTTCTGTGGCTGTTTTCGTCTCTGCCGCCATGCCTGGCCCATTCCTTAATGCTTCTTTTCTTCTTCACGAGCTTCGCAAGAGGATAGATTTTGGGCCAGACACCCGTTATACATATGGTAATGGGCCCAAAAACCCTCCGACAACTTTGACATTCGGCCCACTGTTTTCAGCATCAAAACTATACCAAAACAGCCCCAAAGAGGACTTGACACTGGCCAACACGTTAATGAGACCAACCCATTTGTTTGAGGCACAACAATGGAGCAAGGATCTACATTTGACGACAGAGAGGTATGGCGCCGTCAAACGAGTTTTCGTGGTTTCAGGGAAGGATATGGTGATCAAAAAGAGCTTCCAACATTGGGTGATCAAAAGAAATCCACCTACTAATGTGTTGGAAGTTGGAGGATCGGATCATATGGTGATGATGTCCAAGCCGTTCGATCTCTTCAAGAAACTCAACCGCATTGCTCGACACTATTCTTAG

Protein sequence

MTYLNLLLLFFFFFLSSTVAKTRSDHPPPGGGGGKHFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS
Homology
BLAST of Sgr021221 vs. NCBI nr
Match: KAG7035115.1 (Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 477.6 bits (1228), Expect = 7.4e-131
Identity = 235/289 (81.31%), Postives = 256/289 (88.58%), Query Frame = 0

Query: 1   MTYLNLLLLFFFFFLSSTVAKTRSDHPPPGGGGGKHFVLVHGACHGAWSWYKLSTMLRLA 60
           M YLN+L+  FF FLSSTV  TRS+ PP GGG GKHFVLVHGAC GAWSWYKLST+LR A
Sbjct: 1   MKYLNILI--FFLFLSSTVTLTRSNPPPSGGGDGKHFVLVHGACLGAWSWYKLSTLLRSA 60

Query: 61  GHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCISKA 120
           GH VTALDMA AGID REAE LRSFSEYV+PLTDFMA+L AEEKVILVGHSQGGLCISKA
Sbjct: 61  GHRVTALDMAGAGIDPREAEDLRSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKA 120

Query: 121 MEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLTFG 180
           MEDFPDK+SVAVFV+AAMPGP LNASFLL + RKRIDFGPDTRYT+GNGPK+PPTTLTFG
Sbjct: 121 MEDFPDKISVAVFVAAAMPGPSLNASFLLEQFRKRIDFGPDTRYTFGNGPKSPPTTLTFG 180

Query: 181 PLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDM 240
           PLFSA+KL+  SPK+DLTL NTLMR THLF  +QWSKDL LTT+RYG+VKRVFVVS KD 
Sbjct: 181 PLFSATKLFDRSPKQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDK 240

Query: 241 VIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           VIKKSFQ WVIKRNPPT+V+EV GSDHMVM+SKPFDLF KL+ IAR YS
Sbjct: 241 VIKKSFQQWVIKRNPPTDVVEVRGSDHMVMISKPFDLFNKLSHIARRYS 287

BLAST of Sgr021221 vs. NCBI nr
Match: XP_023534036.1 (salicylic acid-binding protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 477.6 bits (1228), Expect = 7.4e-131
Identity = 233/289 (80.62%), Postives = 255/289 (88.24%), Query Frame = 0

Query: 1   MTYLNLLLLFFFFFLSSTVAKTRSDHPPPGGGGGKHFVLVHGACHGAWSWYKLSTMLRLA 60
           M YLN+L+  FF FLSSTV  TRS+ PP GGG GKHFVLVHGAC GAWSWYKLST+LR A
Sbjct: 1   MKYLNILI--FFLFLSSTVTLTRSNPPPSGGGDGKHFVLVHGACLGAWSWYKLSTLLRSA 60

Query: 61  GHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCISKA 120
           GH VTALDM  AGID REAE LRSFSEYV+PLTDFMA+L AEEKVILVGHSQGGLCISKA
Sbjct: 61  GHRVTALDMVGAGIDPREAEDLRSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKA 120

Query: 121 MEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLTFG 180
           MEDFPDK+SVAVFV+AAMPGP LNASFLL + RKRIDFGPDTRYT+GNGPK+PPTTL FG
Sbjct: 121 MEDFPDKISVAVFVAAAMPGPSLNASFLLEQFRKRIDFGPDTRYTFGNGPKSPPTTLRFG 180

Query: 181 PLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDM 240
           PLFSA+KL+  SP +DLTL NTLMR THLF  +QWSKDL LTT+RYG+VKRVFVVS KD 
Sbjct: 181 PLFSATKLFDRSPNQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDK 240

Query: 241 VIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           VIKKSFQHWVIKRNPPT+V+E+ GSDHMVM+SKPFDLF KL+ IARHYS
Sbjct: 241 VIKKSFQHWVIKRNPPTDVVEIRGSDHMVMISKPFDLFNKLSHIARHYS 287

BLAST of Sgr021221 vs. NCBI nr
Match: KAG6605115.1 (Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 476.1 bits (1224), Expect = 2.2e-130
Identity = 233/289 (80.62%), Postives = 255/289 (88.24%), Query Frame = 0

Query: 1   MTYLNLLLLFFFFFLSSTVAKTRSDHPPPGGGGGKHFVLVHGACHGAWSWYKLSTMLRLA 60
           M YLN+L+  FF F+SSTV  TRS+ PP GGG GKHFVLVHGAC GAWSWYKLST+LR A
Sbjct: 1   MKYLNILI--FFLFVSSTVTLTRSNPPPSGGGDGKHFVLVHGACLGAWSWYKLSTLLRSA 60

Query: 61  GHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCISKA 120
           GH VTALDM  AGID REAE LRSFSEYV+PLTDFMA+L AEEKVILVGHSQGGLCISKA
Sbjct: 61  GHRVTALDMVGAGIDPREAEDLRSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKA 120

Query: 121 MEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLTFG 180
           MEDFPDK+SVAVFV+AAMPGP LNASFLL + RKRIDFGPDTRYT+GNGPK+PPTTLTFG
Sbjct: 121 MEDFPDKISVAVFVAAAMPGPSLNASFLLEQFRKRIDFGPDTRYTFGNGPKSPPTTLTFG 180

Query: 181 PLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDM 240
           PLFSA+KL+  SPK+DLTL NTLMR THLF  +QWSKDL LTT+RYG+VKRVFVVS KD 
Sbjct: 181 PLFSATKLFDRSPKQDLTLGNTLMRKTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDK 240

Query: 241 VIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           VIKKSFQ WVIKRNPPT+V+E+ GSDHMVMMSKPFDLF KL+ IAR YS
Sbjct: 241 VIKKSFQQWVIKRNPPTDVVEIRGSDHMVMMSKPFDLFNKLSHIARRYS 287

BLAST of Sgr021221 vs. NCBI nr
Match: XP_022947435.1 (salicylic acid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 474.9 bits (1221), Expect = 4.8e-130
Identity = 234/289 (80.97%), Postives = 254/289 (87.89%), Query Frame = 0

Query: 1   MTYLNLLLLFFFFFLSSTVAKTRSDHPPPGGGGGKHFVLVHGACHGAWSWYKLSTMLRLA 60
           M YLN+L+  FF FLSSTV  TRS+ PP GGG GKHFVLVHGAC GAWSWYKLST+LR  
Sbjct: 1   MKYLNILI--FFLFLSSTVTLTRSNPPPSGGGDGKHFVLVHGACLGAWSWYKLSTLLRSV 60

Query: 61  GHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCISKA 120
           GH VTALDMA AGID REAE LRSFSEYV+PLTDFMA+L AEEKVILVGHSQGGLCISKA
Sbjct: 61  GHRVTALDMAGAGIDPREAEDLRSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKA 120

Query: 121 MEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLTFG 180
           MEDFPDK+SVAVFV AAMPGP LNASFLL + RKRIDFGPDTRYT+GNGPK+PPTTLTFG
Sbjct: 121 MEDFPDKISVAVFVVAAMPGPSLNASFLLEQFRKRIDFGPDTRYTFGNGPKSPPTTLTFG 180

Query: 181 PLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDM 240
           PLFSA+KL+  SPK+DLTL NTLMR THLF  +QWSKDL LTT+RYG+VKRVFVVS KD 
Sbjct: 181 PLFSATKLFDRSPKQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDK 240

Query: 241 VIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           VIKKSFQ WVIKRNPPT+V+EV GSDHMVM+SKPFDLF KL+ IAR YS
Sbjct: 241 VIKKSFQQWVIKRNPPTDVVEVRGSDHMVMISKPFDLFNKLSHIARRYS 287

BLAST of Sgr021221 vs. NCBI nr
Match: XP_023007515.1 (salicylic acid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 469.9 bits (1208), Expect = 1.5e-128
Identity = 231/289 (79.93%), Postives = 253/289 (87.54%), Query Frame = 0

Query: 1   MTYLNLLLLFFFFFLSSTVAKTRSDHPPPGGGGGKHFVLVHGACHGAWSWYKLSTMLRLA 60
           M YLN+L+  FF FLSSTV  TRS+ PP GGG GKHFVLVHGAC GAWSWYKLST+LR A
Sbjct: 1   MQYLNILI--FFLFLSSTVTLTRSNPPPSGGGDGKHFVLVHGACLGAWSWYKLSTLLRSA 60

Query: 61  GHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCISKA 120
           GH VTALDMA AGID REAE L+SFSEYV+PLTDFMA+L AEEKVILVGHSQGGLCISKA
Sbjct: 61  GHRVTALDMAGAGIDPREAEDLKSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKA 120

Query: 121 MEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLTFG 180
           MEDFPDK+SVAVFV+AAMPGP LNASFLL +  KRIDFGPD+RYT+GNGPK+PPTTL FG
Sbjct: 121 MEDFPDKISVAVFVAAAMPGPSLNASFLLEQFSKRIDFGPDSRYTFGNGPKSPPTTLKFG 180

Query: 181 PLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDM 240
           PLFSA+KL+  SPK+DLTL NTLMR THLF  +QWSKDL LTT+RYG+VKRVFVVS KD 
Sbjct: 181 PLFSATKLFDRSPKQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDK 240

Query: 241 VIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           VIKKSFQ WVIKRNPP +V+EV GSDHMVMMSKPFDLF KL+ IAR YS
Sbjct: 241 VIKKSFQQWVIKRNPPNDVVEVRGSDHMVMMSKPFDLFNKLSHIARRYS 287

BLAST of Sgr021221 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 5.8e-62
Identity = 123/258 (47.67%), Postives = 166/258 (64.34%), Query Frame = 0

Query: 34  GKHFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGIDRREAESLRSFSEYVEPLT 93
           GKHFVLVHGACHG WSWYKL  +L  AGH VTALD+AA+G D R+ E LR+  +Y  PL 
Sbjct: 4   GKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPLM 63

Query: 94  DFMAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELR 153
           + M  L+A+EKVILVGHS GG+ +  AME +P K+  AVF++A MP    N+SF+L +  
Sbjct: 64  ELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQYN 123

Query: 154 KR--IDFGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLFE 213
           +R   +   DT++     P+ P T++ FGP F A KLYQ    EDL LA++L+RP+ LF 
Sbjct: 124 ERTPAENWLDTQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPSSLF- 183

Query: 214 AQQWSKDLHLTTERYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMVMM 273
            +  SK  + T ER+G+VKRV++V  +D  I + FQ W I     T  +E+ G+DHM M+
Sbjct: 184 MEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADHMAML 243

Query: 274 SKPFDLFKKLNRIARHYS 290
            +P  L   L  IA  Y+
Sbjct: 244 CEPQKLCASLLEIAHKYN 260

BLAST of Sgr021221 vs. ExPASy Swiss-Prot
Match: Q8S9K8 (Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.3e-56
Identity = 112/256 (43.75%), Postives = 155/256 (60.55%), Query Frame = 0

Query: 36  HFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDF 95
           HFV VHG+CHGAW W+KL+  L+L GH VTA+D+  +G+D R+   +R  S Y+EPL  F
Sbjct: 21  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 80

Query: 96  MAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKR 155
           M  L   EKV+LVGHS GG+  S AME FP KVSV +F+SA MP      + L+ E   R
Sbjct: 81  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTR 140

Query: 156 I--DFGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQ 215
           +   F  D  +T+  G ++PP+++ FG  F   K Y N   EDL LA  LM+P+ L+  +
Sbjct: 141 LPEGFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLEDLELAMALMKPSWLYTKE 200

Query: 216 QWSKDLHLTTERYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSK 275
              +DL +T ERYG+ KRVF+V   D V+ +  Q W+I    P  V  +  + HM M++K
Sbjct: 201 MGGEDL-ITKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRIEEAGHMAMLTK 260

Query: 276 PFDLFKKLNRIARHYS 290
           P +L + L  IA  Y+
Sbjct: 261 PHELSQLLQEIAAKYN 275

BLAST of Sgr021221 vs. ExPASy Swiss-Prot
Match: A2WYS8 (Probable esterase PIR7A OS=Oryza sativa subsp. indica OX=39946 GN=PIR7A PE=3 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 8.1e-56
Identity = 122/259 (47.10%), Postives = 151/259 (58.30%), Query Frame = 0

Query: 33  GGKHFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGIDRREAESLRSFSEYVEPL 92
           GGKHFV VHG  HGAW WY++   LR AGH  TALDMAAAG     A+ + S  EY  PL
Sbjct: 4   GGKHFVFVHGLGHGAWCWYRVVAALRAAGHRATALDMAAAGAHPARADEVGSLEEYSRPL 63

Query: 93  TDFMAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHEL 152
            D +A  A  E+++LVGHS GGL ++ AME FPDKV+ AVF++A MP    +    L E 
Sbjct: 64  LDAVAAAAPGERLVLVGHSLGGLSLALAMERFPDKVAAAVFLAACMPAAGKHMGITLEEF 123

Query: 153 RKRI--DFGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLF 212
            +RI  DF  D++    N  + P T +  GP   A KLY  SP EDLTLA  L+RP   +
Sbjct: 124 MRRIKPDFFMDSKTIVLNTNQEPRTAVLLGPKLLAEKLYNRSPPEDLTLATMLVRPGTNY 183

Query: 213 EAQQWSKDLHLTTE-RYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMV 272
                 KD  L TE  YG+VKRVF+V+  D    +  Q W I  +P   V E+ G+DHM 
Sbjct: 184 IDDPIMKDETLLTEGNYGSVKRVFLVAMDDASSDEEMQRWTIDLSPGVEVEELAGADHMA 243

Query: 273 MMSKPFDLFKKLNRIARHY 289
           M SKP +L   L RIA  Y
Sbjct: 244 MCSKPRELCDLLLRIAAKY 262

BLAST of Sgr021221 vs. ExPASy Swiss-Prot
Match: Q0JG98 (Probable esterase PIR7A OS=Oryza sativa subsp. japonica OX=39947 GN=PIR7A PE=3 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 8.1e-56
Identity = 122/259 (47.10%), Postives = 151/259 (58.30%), Query Frame = 0

Query: 33  GGKHFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGIDRREAESLRSFSEYVEPL 92
           GGKHFV VHG  HGAW WY++   LR AGH  TALDMAAAG     A+ + S  EY  PL
Sbjct: 4   GGKHFVFVHGLGHGAWCWYRVVAALRAAGHRATALDMAAAGAHPARADEVGSLEEYSRPL 63

Query: 93  TDFMAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHEL 152
            D +A  A  E+++LVGHS GGL ++ AME FPDKV+ AVF++A MP    +    L E 
Sbjct: 64  LDAVAAAAPGERLVLVGHSLGGLSLALAMERFPDKVAAAVFLAACMPAAGKHMGITLEEF 123

Query: 153 RKRI--DFGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLF 212
            +RI  DF  D++    N  + P T +  GP   A KLY  SP EDLTLA  L+RP   +
Sbjct: 124 MRRIKPDFFMDSKTIVLNTNQEPRTAVLLGPKLLAEKLYNRSPPEDLTLATMLVRPGTNY 183

Query: 213 EAQQWSKDLHLTTE-RYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMV 272
                 KD  L TE  YG+VKRVF+V+  D    +  Q W I  +P   V E+ G+DHM 
Sbjct: 184 IDDPIMKDETLLTEGNYGSVKRVFLVAMDDASSDEEMQRWTIDLSPGVEVEELAGADHMA 243

Query: 273 MMSKPFDLFKKLNRIARHY 289
           M SKP +L   L RIA  Y
Sbjct: 244 MCSKPRELCDLLLRIAAKY 262

BLAST of Sgr021221 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.8e-54
Identity = 110/250 (44.00%), Postives = 158/250 (63.20%), Query Frame = 0

Query: 35  KHFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGIDRREAESLRSFSEYVEPLTD 94
           KHFVLVHG C GAW WYKL  +L  AGH VTA+D++AAGI+ R  + + +F +Y EPL +
Sbjct: 11  KHFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPLME 70

Query: 95  FMAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRK 154
            MA +  +EKV+L+GHS GG+ +  AME +P+K+SVAVF+SA MP P  + ++   +  +
Sbjct: 71  VMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKYNE 130

Query: 155 R--IDFGPDTRY-TYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLFE 214
           +   D   D+++ TYGN P+NP  ++  GP F A K++QN   EDL LA  L RP  LF 
Sbjct: 131 KCPADMMLDSQFSTYGN-PENPGMSMILGPQFMALKMFQNCSVEDLELAKMLTRPGSLF- 190

Query: 215 AQQWSKDLHLTTERYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMVMM 274
            Q  +K    +TERYG+VKR ++   +D      FQ W ++      V E+  +DHM M+
Sbjct: 191 FQDLAKAKKFSTERYGSVKRAYIFCNEDKSFPVEFQKWFVESVGADKVKEIKEADHMGML 250

Query: 275 SKPFDLFKKL 282
           S+P ++ K L
Sbjct: 251 SQPREVCKCL 258

BLAST of Sgr021221 vs. ExPASy TrEMBL
Match: A0A6J1G6W0 (salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111451294 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.3e-130
Identity = 234/289 (80.97%), Postives = 254/289 (87.89%), Query Frame = 0

Query: 1   MTYLNLLLLFFFFFLSSTVAKTRSDHPPPGGGGGKHFVLVHGACHGAWSWYKLSTMLRLA 60
           M YLN+L+  FF FLSSTV  TRS+ PP GGG GKHFVLVHGAC GAWSWYKLST+LR  
Sbjct: 1   MKYLNILI--FFLFLSSTVTLTRSNPPPSGGGDGKHFVLVHGACLGAWSWYKLSTLLRSV 60

Query: 61  GHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCISKA 120
           GH VTALDMA AGID REAE LRSFSEYV+PLTDFMA+L AEEKVILVGHSQGGLCISKA
Sbjct: 61  GHRVTALDMAGAGIDPREAEDLRSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKA 120

Query: 121 MEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLTFG 180
           MEDFPDK+SVAVFV AAMPGP LNASFLL + RKRIDFGPDTRYT+GNGPK+PPTTLTFG
Sbjct: 121 MEDFPDKISVAVFVVAAMPGPSLNASFLLEQFRKRIDFGPDTRYTFGNGPKSPPTTLTFG 180

Query: 181 PLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDM 240
           PLFSA+KL+  SPK+DLTL NTLMR THLF  +QWSKDL LTT+RYG+VKRVFVVS KD 
Sbjct: 181 PLFSATKLFDRSPKQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDK 240

Query: 241 VIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           VIKKSFQ WVIKRNPPT+V+EV GSDHMVM+SKPFDLF KL+ IAR YS
Sbjct: 241 VIKKSFQQWVIKRNPPTDVVEVRGSDHMVMISKPFDLFNKLSHIARRYS 287

BLAST of Sgr021221 vs. ExPASy TrEMBL
Match: A0A6J1L366 (salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111499981 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 7.5e-129
Identity = 231/289 (79.93%), Postives = 253/289 (87.54%), Query Frame = 0

Query: 1   MTYLNLLLLFFFFFLSSTVAKTRSDHPPPGGGGGKHFVLVHGACHGAWSWYKLSTMLRLA 60
           M YLN+L+  FF FLSSTV  TRS+ PP GGG GKHFVLVHGAC GAWSWYKLST+LR A
Sbjct: 1   MQYLNILI--FFLFLSSTVTLTRSNPPPSGGGDGKHFVLVHGACLGAWSWYKLSTLLRSA 60

Query: 61  GHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCISKA 120
           GH VTALDMA AGID REAE L+SFSEYV+PLTDFMA+L AEEKVILVGHSQGGLCISKA
Sbjct: 61  GHRVTALDMAGAGIDPREAEDLKSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKA 120

Query: 121 MEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLTFG 180
           MEDFPDK+SVAVFV+AAMPGP LNASFLL +  KRIDFGPD+RYT+GNGPK+PPTTL FG
Sbjct: 121 MEDFPDKISVAVFVAAAMPGPSLNASFLLEQFSKRIDFGPDSRYTFGNGPKSPPTTLKFG 180

Query: 181 PLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDM 240
           PLFSA+KL+  SPK+DLTL NTLMR THLF  +QWSKDL LTT+RYG+VKRVFVVS KD 
Sbjct: 181 PLFSATKLFDRSPKQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDK 240

Query: 241 VIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           VIKKSFQ WVIKRNPP +V+EV GSDHMVMMSKPFDLF KL+ IAR YS
Sbjct: 241 VIKKSFQQWVIKRNPPNDVVEVRGSDHMVMMSKPFDLFNKLSHIARRYS 287

BLAST of Sgr021221 vs. ExPASy TrEMBL
Match: A0A6J1D5N6 (salicylic acid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC111017157 PE=4 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.9e-124
Identity = 223/284 (78.52%), Postives = 245/284 (86.27%), Query Frame = 0

Query: 6   LLLLFFFFFLSSTVAKTRSDHPPPGGGGGKHFVLVHGACHGAWSWYKLSTMLRLAGHSVT 65
           LLLLF F FL S+VAKTRSD  P       HFVLVHGACHGAWSWYK++ +LR AGH VT
Sbjct: 7   LLLLFLFLFLCSSVAKTRSDPAP----RRHHFVLVHGACHGAWSWYKVAALLRSAGHRVT 66

Query: 66  ALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCISKAMEDFP 125
           A+DMA +GIDRREAE LRSFSEYV PL D MA +AAEEKV+LVGHSQGGLCISKAME FP
Sbjct: 67  AVDMAGSGIDRREAERLRSFSEYVGPLRDAMAAVAAEEKVVLVGHSQGGLCISKAMESFP 126

Query: 126 DKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLTFGPLFSA 185
           DK+SVAVFV+AAMPGP LN SFLL ++ +R+DFGPD+RYTYGNGP+NPPTTLTFGPLFSA
Sbjct: 127 DKISVAVFVAAAMPGPSLNVSFLLQQMLRRVDFGPDSRYTYGNGPRNPPTTLTFGPLFSA 186

Query: 186 SKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDMVIKKS 245
           SKLY NSPKEDLTLANTLMRPTHLF  QQWSKDL+LT ERYG+VKRVFVVSG D +I K 
Sbjct: 187 SKLYNNSPKEDLTLANTLMRPTHLFGTQQWSKDLNLTVERYGSVKRVFVVSGNDRIINKK 246

Query: 246 FQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           FQ WVIKRNPPT V+EV GSDHMVMMSKPF LFKKL+RIAR Y+
Sbjct: 247 FQWWVIKRNPPTAVVEVRGSDHMVMMSKPFHLFKKLHRIARDYA 286

BLAST of Sgr021221 vs. ExPASy TrEMBL
Match: A0A0A0LL04 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G237140 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 5.0e-117
Identity = 216/295 (73.22%), Postives = 243/295 (82.37%), Query Frame = 0

Query: 1   MTYLNLLLLFFFF---FLSSTVAKTRSDHPPP---GGGGGKHFVLVHGACHGAWSWYKLS 60
           M  LNLLLLFFFF   FLS T+ K+ SD PP    GGGGGKHFVLVHGAC GAWSWYKLS
Sbjct: 1   MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS 60

Query: 61  TMLRLAGHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGG 120
           T+LR AGH VTALDMA AGID REAE L+SF+EYVEPL + M E+  EEKVILVGHSQGG
Sbjct: 61  TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG 120

Query: 121 LCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPP 180
           LCISKAME+FP+K+SVAVFV AAMPGP LNASFL+ +LRK +DFGPD+ YTYGNGP++PP
Sbjct: 121 LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP 180

Query: 181 TTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFV 240
           TTLTFGPLF A+K++  SP EDLTL  TL+RPTHLF  +QW+KDL LT ERYG+VKRVFV
Sbjct: 181 TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV 240

Query: 241 VSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           VS  D VIKKSFQ WVI+RNPP  V+EV GSDHMVMMSKP  LF  L+ IAR +S
Sbjct: 241 VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295

BLAST of Sgr021221 vs. ExPASy TrEMBL
Match: A0A1S3C9T3 (salicylic acid-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103498416 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 2.1e-115
Identity = 214/291 (73.54%), Postives = 239/291 (82.13%), Query Frame = 0

Query: 1   MTYLNLLLLFFFFFLSSTVAKTRSDHPPPG--GGGGKHFVLVHGACHGAWSWYKLSTMLR 60
           M YLN  LLFFF FLS T+ K+ SD PP      GGKHFVLVHGAC GAWSWYKLST+LR
Sbjct: 1   MHYLN--LLFFFLFLSPTLTKSLSDPPPSPDVSSGGKHFVLVHGACLGAWSWYKLSTLLR 60

Query: 61  LAGHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDFMAELAAEEKVILVGHSQGGLCIS 120
            AGH VTALDMA AGID REAESL +F+EYVEPL  FMAE+  +EKVILVGHSQGGLCIS
Sbjct: 61  SAGHRVTALDMAGAGIDPREAESL-TFNEYVEPLRSFMAEVGEDEKVILVGHSQGGLCIS 120

Query: 121 KAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKRIDFGPDTRYTYGNGPKNPPTTLT 180
           KAME++P+K+ VAVFV AAMPGP LNASFL+ ELRKR+DFGPD+RYTYGNGP +PPTTLT
Sbjct: 121 KAMEEYPEKICVAVFVVAAMPGPALNASFLIGELRKRLDFGPDSRYTYGNGPTSPPTTLT 180

Query: 181 FGPLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGK 240
           FGPLF A+ ++  SP EDLTL   L+RPTHLF  +QW+KDL LT ERYGAVKRVFVVS K
Sbjct: 181 FGPLFLAASVFNKSPPEDLTLGRILVRPTHLFGGEQWNKDLVLTKERYGAVKRVFVVSDK 240

Query: 241 DMVIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKPFDLFKKLNRIARHYS 290
           D VIKK+FQ WVI+RNPP  V+EV GSDHMVMMSKP DLF  L+ IAR YS
Sbjct: 241 DKVIKKNFQKWVIRRNPPNGVVEVKGSDHMVMMSKPLDLFNILSHIARQYS 288

BLAST of Sgr021221 vs. TAIR 10
Match: AT3G50440.1 (methyl esterase 10 )

HSP 1 Score: 221.5 bits (563), Expect = 8.9e-58
Identity = 112/256 (43.75%), Postives = 155/256 (60.55%), Query Frame = 0

Query: 36  HFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGIDRREAESLRSFSEYVEPLTDF 95
           HFV VHG+CHGAW W+KL+  L+L GH VTA+D+  +G+D R+   +R  S Y+EPL  F
Sbjct: 34  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 93

Query: 96  MAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRKR 155
           M  L   EKV+LVGHS GG+  S AME FP KVSV +F+SA MP      + L+ E   R
Sbjct: 94  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTR 153

Query: 156 I--DFGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQ 215
           +   F  D  +T+  G ++PP+++ FG  F   K Y N   EDL LA  LM+P+ L+  +
Sbjct: 154 LPEGFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLEDLELAMALMKPSWLYTKE 213

Query: 216 QWSKDLHLTTERYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSK 275
              +DL +T ERYG+ KRVF+V   D V+ +  Q W+I    P  V  +  + HM M++K
Sbjct: 214 MGGEDL-ITKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRIEEAGHMAMLTK 273

Query: 276 PFDLFKKLNRIARHYS 290
           P +L + L  IA  Y+
Sbjct: 274 PHELSQLLQEIAAKYN 288

BLAST of Sgr021221 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 205.3 bits (521), Expect = 6.6e-53
Identity = 114/258 (44.19%), Postives = 154/258 (59.69%), Query Frame = 0

Query: 35  KHFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGID-RREAESLRSFSEYVEPLT 94
           +H VLVHGACHGAW WYK+   L  +GH VTA+D+AA+GID  R    + +  +Y EPL 
Sbjct: 8   QHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEPLM 67

Query: 95  DFMAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELR 154
             M  L  +EKV+LVGHS GGL ++ AM+ FP K+SV+VFV+A MP    + SF+  +LR
Sbjct: 68  QLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDKLR 127

Query: 155 KRI--DFGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLFE 214
           K    +   DT +T    P  P     FGP F A  LYQ SP +DL LA  L+R   L +
Sbjct: 128 KETSREEWLDTVFT-SEKPDFPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLVRANPLIK 187

Query: 215 AQQWSKDLHLTTERYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMVMM 274
            +  ++    + E YG+V R+F+V GKD+V  + +Q  +I   PP  V+E+  +DHM M 
Sbjct: 188 -KDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIKDADHMPMF 247

Query: 275 SKPFDLFKKLNRIARHYS 290
           SKP  L   L  IA  Y+
Sbjct: 248 SKPQQLCALLLEIANKYA 263

BLAST of Sgr021221 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-48
Identity = 111/260 (42.69%), Postives = 148/260 (56.92%), Query Frame = 0

Query: 35  KHFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGID-RREAESLRSFSEYVEPLT 94
           +HFVLVHG+CHGAW WYK+  +L   GH VTA+D+AA+GID  R    + +  +Y EPLT
Sbjct: 8   QHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPLT 67

Query: 95  DFMAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELR 154
             +  L  +EKV+LVGHS GGL ++ AME FP+K+SVAVF++A MP    + SF+L +  
Sbjct: 68  KLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKFG 127

Query: 155 KRI--------DFGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMR 214
             +        +F P        G  N   ++ F P F    LYQ SP EDL L   LMR
Sbjct: 128 SNMPQEAWMGTEFEP-------YGSDNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMR 187

Query: 215 PTHLFEAQQWSKDLHLTTERYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGS 274
           P  LF     SK  + + E YG+V RVF+V  +D  I +  Q W+I   P   V+E+  +
Sbjct: 188 PGSLF-INDLSKMKNFSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEET 247

Query: 275 DHMVMMSKPFDLFKKLNRIA 286
           DHM M  KP  L     +IA
Sbjct: 248 DHMPMFCKPQQLSDYFLKIA 259

BLAST of Sgr021221 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 190.3 bits (482), Expect = 2.2e-48
Identity = 109/259 (42.08%), Postives = 151/259 (58.30%), Query Frame = 0

Query: 35  KHFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGID-RREAESLRSFSEYVEPLT 94
           +HFVLVHGACHGAW WYK+  +L   GH VTALD+AA+GID  R    + +  +Y EPL 
Sbjct: 8   QHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLM 67

Query: 95  DFMAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELR 154
             M  L  +EKV+LVGHS GGL ++ AM+ FPDK+SV+VFV+A MP    + SF+  +  
Sbjct: 68  QLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFA 127

Query: 155 KRID----FGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHL 214
             +      G +   TYG+   N   ++ F   F   +LYQ SP EDL L   L RP+ L
Sbjct: 128 SSMTPEGWMGSELE-TYGS--DNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSL 187

Query: 215 FEAQQWSKDLHLTTERYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMV 274
           F   + SK  + + + YG+V R ++V  +D +I +  Q W+I   P   V+E+  +DHM 
Sbjct: 188 F-INELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMP 247

Query: 275 MMSKPFDLFKKLNRIARHY 289
           M  KP  L   L  IA ++
Sbjct: 248 MFCKPQLLSDHLLAIADNF 262

BLAST of Sgr021221 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 187.2 bits (474), Expect = 1.9e-47
Identity = 103/255 (40.39%), Postives = 146/255 (57.25%), Query Frame = 0

Query: 35  KHFVLVHGACHGAWSWYKLSTMLRLAGHSVTALDMAAAGIDRREAESLRSFSEYVEPLTD 94
           +HFVLVHG+C GAW WYK+  +L  +GH VTALD+AA GID R    + +  +Y EPL  
Sbjct: 26  QHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSEPLIQ 85

Query: 95  FMAELAAEEKVILVGHSQGGLCISKAMEDFPDKVSVAVFVSAAMPGPFLNASFLLHELRK 154
            M  L  +EKV+LVGHS GGL ++ AM+ FPDK+SV+VFV++ MP    + SF+L +   
Sbjct: 86  LMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPDTKNSPSFVLEKFAS 145

Query: 155 RIDFGPDTRYTYGNGPKNPPTTLTFGPLFSASKLYQNSPKEDLTLANTLMRPTHLFEAQQ 214
            +     T   +      P   + F   F+  ++ Q SP EDL L   L RP  LF    
Sbjct: 146 TM-----TPEDWMGSELEP--YVVFSAEFTKHRILQLSPIEDLELRLLLKRPGSLF-LND 205

Query: 215 WSKDLHLTTERYGAVKRVFVVSGKDMVIKKSFQHWVIKRNPPTNVLEVGGSDHMVMMSKP 274
            S+  + + + YG+V R ++VS  D  I + +Q W+I   PP  V+E+ G+DH+ +  KP
Sbjct: 206 LSRMKNFSEKGYGSVPRAYIVSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLPLFCKP 265

Query: 275 FDLFKKLNRIARHYS 290
             L   L  IA  +S
Sbjct: 266 QLLSDHLLAIADKFS 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035115.17.4e-13181.31Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_023534036.17.4e-13180.62salicylic acid-binding protein 2-like [Cucurbita pepo subsp. pepo][more]
KAG6605115.12.2e-13080.62Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022947435.14.8e-13080.97salicylic acid-binding protein 2-like [Cucurbita moschata][more]
XP_023007515.11.5e-12879.93salicylic acid-binding protein 2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q6RYA05.8e-6247.67Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1[more]
Q8S9K81.3e-5643.75Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1[more]
A2WYS88.1e-5647.10Probable esterase PIR7A OS=Oryza sativa subsp. indica OX=39946 GN=PIR7A PE=3 SV=... [more]
Q0JG988.1e-5647.10Probable esterase PIR7A OS=Oryza sativa subsp. japonica OX=39947 GN=PIR7A PE=3 S... [more]
Q9SE935.8e-5444.00Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1G6W02.3e-13080.97salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111451... [more]
A0A6J1L3667.5e-12979.93salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149998... [more]
A0A6J1D5N61.9e-12478.52salicylic acid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A0A0LL045.0e-11773.22AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G237... [more]
A0A1S3C9T32.1e-11573.54salicylic acid-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103498416 PE... [more]
Match NameE-valueIdentityDescription
AT3G50440.18.9e-5843.75methyl esterase 10 [more]
AT2G23610.16.6e-5344.19methyl esterase 3 [more]
AT2G23620.11.3e-4842.69methyl esterase 1 [more]
AT2G23600.12.2e-4842.08acetone-cyanohydrin lyase [more]
AT2G23590.11.9e-4740.39methyl esterase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 34..289
e-value: 1.3E-65
score: 223.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 28..282
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 37..277
e-value: 1.5E-19
score: 71.5
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 34..287
NoneNo IPR availablePANTHERPTHR10992:SF1002ESTERASE PIR7A-RELATEDcoord: 34..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021221.1Sgr021221.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity