Sgr021188 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr021188 vs. NCBI nr
Match: KAG6579345.1 (hypothetical protein SDJN03_23793, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 142.9 bits (359), Expect = 2.6e-30
Identity = 81/178 (45.51%), Postives = 116/178 (65.17%), Query Frame = 0

Query: 7   EVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWARFFFFAISR-------SSLTCN 66
           E+++  E++  M E +EA++ YY+TK+ NII+AYFVW R FFF IS+       S+L+CN
Sbjct: 9   ELKKMMEEVQGMGENVEAKVNYYDTKLHNIIVAYFVWERVFFFGISKKTFPPTISTLSCN 68

Query: 67  G-WWVTFALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREEANE-- 126
           G WWV  AL+S C+FVY +LF D+ LMLYR E+ L ++ Q+ A+  R +   +EE  +  
Sbjct: 69  GNWWVILALSSSCSFVYVLLFFDTALMLYRHENQLHLILQKHAQLCRHLLAIKEEQADIK 128

Query: 127 ----EGEDDSSHEVELIREMILTNSNYTRSSRRGMERKVYIYSISGALVGVAATELYA 171
               E  D++SH + L  E++L NS      RR  ERKVY+Y+I  AL+GVA+ ELYA
Sbjct: 129 ASLMEAGDEASHGLSLEEELMLINSTSPFRRRRPWERKVYVYTIFCALIGVASLELYA 186

BLAST of Sgr021188 vs. NCBI nr
Match: KAG6579352.1 (hypothetical protein SDJN03_23800, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 136.3 bits (342), Expect = 2.4e-28
Identity = 78/178 (43.82%), Postives = 113/178 (63.48%), Query Frame = 0

Query: 7   EVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWARFFFFAISR-------SSLTCN 66
           E+++  E++  M EK+EA++ YY+TK+  II+AYFVW R FFF IS+       S+L+CN
Sbjct: 5   ELKKMMEEVQGMGEKVEAKVNYYDTKLHTIIVAYFVWERVFFFGISKKTFPSTISTLSCN 64

Query: 67  G-WWVTFALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREEANE-- 126
           G WWV  AL+  C+FVY +LF D+ LMLYR E+ L ++ Q+ A+  R +   +EE  +  
Sbjct: 65  GNWWVILALSCSCSFVYVLLFFDAALMLYRHENQLHLILQKHAQLCRHLLAIKEEQADTK 124

Query: 127 ----EGEDDSSHEVELIREMILTNSNYTRSSRRGMERKVYIYSISGALVGVAATELYA 171
               E  D +SH + L  E++L NS      RR  ERKV++Y+I  A +GVA+ ELYA
Sbjct: 125 ASLMEAGDQASHGLSLEEELMLINSTSPFRRRRPWERKVHVYTIFCAFIGVASLELYA 182

BLAST of Sgr021188 vs. NCBI nr
Match: KAG6579344.1 (hypothetical protein SDJN03_23792, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 127.9 bits (320), Expect = 8.5e-26
Identity = 76/177 (42.94%), Postives = 113/177 (63.84%), Query Frame = 0

Query: 7   EVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWAR-----FFFFAISR-------S 66
           ++E+R E++  M EK+E R+ YY+TK+  II+AY VW R     FFFF +S        S
Sbjct: 6   QMEKRMEEVKGMWEKVEGRVNYYDTKLHAIIVAYLVWERVFFFFFFFFGVSNTNFASNIS 65

Query: 67  SLTCNG-WWVTFALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREE 126
           +L+CNG WWV  AL+ LC+FVY +LF+D+ LMLY  ++ L+++ Q   + YR++   ++ 
Sbjct: 66  TLSCNGKWWVIVALSCLCSFVYMLLFVDAALMLYPHQNQLNLILQTHHQLYRQLLAIKDS 125

Query: 127 ANEEGEDDSSHEVELIREMILTNSNYTRSSRRGMERKVYIYSISGALVGVAATELYA 171
             E G D++SH + L  E++L NSN     RR   RK Y+Y+I  AL+ VA+ ELYA
Sbjct: 126 LMEAG-DEASHGLSLEEELMLINSN-AAYRRRPWGRKFYVYTIFCALIDVASLELYA 180

BLAST of Sgr021188 vs. NCBI nr
Match: XP_022157182.1 (uncharacterized protein LOC111023958 [Momordica charantia] >XP_022157183.1 uncharacterized protein LOC111023958 [Momordica charantia])

HSP 1 Score: 126.3 bits (316), Expect = 2.5e-25
Identity = 72/170 (42.35%), Postives = 108/170 (63.53%), Query Frame = 0

Query: 7   EVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWARFFFFAISR--SSLTCNGWWVT 66
           E+ER+FE+L  ++EK E+R+RYYETKVQNI+  Y ++ R FFF IS+  SS  C  WWV 
Sbjct: 5   ELERKFEELKDINEKQESRVRYYETKVQNIVFGYLIFTRLFFFGISQTSSSFNCKDWWVI 64

Query: 67  FALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREE-----ANEEGE 126
            AL+ LC+F+YF+LFLD+V ML+R ++ LD++ +E  E +++I +++ +     + E GE
Sbjct: 65  LALSLLCSFIYFLLFLDAVAMLFRTQYQLDIICKELKELFQQILVSKNQDDVGLSMETGE 124

Query: 127 DDSSHEVELIREMILTNSNYTRSSRRGMERKVYIYSISGALVGVAATELY 170
                E     +M++ +        R + RKVYIY    AL+ V A ELY
Sbjct: 125 SSGGFEFGFHEKMLMLD------HFRIVGRKVYIYFTVSALLAVTAIELY 168

BLAST of Sgr021188 vs. NCBI nr
Match: XP_022157130.1 (uncharacterized protein LOC111023927 [Momordica charantia])

HSP 1 Score: 113.6 bits (283), Expect = 1.7e-21
Identity = 68/175 (38.86%), Postives = 104/175 (59.43%), Query Frame = 0

Query: 5   FREVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWARFFFFAISRSS---LTCNGW 64
           F E++R FE L  + EK E+R++Y+E++ QNI +AY +W R FFFAIS++S   L C  W
Sbjct: 3   FGELKRNFEALKDLVEKQESRVQYHESRAQNITMAYLIWGRLFFFAISQTSSSLLKCIDW 62

Query: 65  WVTFALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREE------AN 124
           W+   L+  C FVYF+ FL++V MLYR +H +D++ +EQAE  ++I + R +      A 
Sbjct: 63  WMVLGLSVSCAFVYFLFFLEAVTMLYRVQHQMDIICKEQAEICQQILVARSQLDDVDLAM 122

Query: 125 EEGEDDSSHEVELIREMILTNSNYTRSSRRGMERKVYIYSISGALVGVAATELYA 171
           E G+     +     +++         + R +ERK YI +   AL+ V A ELYA
Sbjct: 123 EAGDSSDGFQFSFHVKLL------EYGAFRIVERKFYICATVSALLAVTAIELYA 171

BLAST of Sgr021188 vs. ExPASy TrEMBL
Match: A0A6J1DSQ0 (uncharacterized protein LOC111023958 OS=Momordica charantia OX=3673 GN=LOC111023958 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.2e-25
Identity = 72/170 (42.35%), Postives = 108/170 (63.53%), Query Frame = 0

Query: 7   EVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWARFFFFAISR--SSLTCNGWWVT 66
           E+ER+FE+L  ++EK E+R+RYYETKVQNI+  Y ++ R FFF IS+  SS  C  WWV 
Sbjct: 5   ELERKFEELKDINEKQESRVRYYETKVQNIVFGYLIFTRLFFFGISQTSSSFNCKDWWVI 64

Query: 67  FALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREE-----ANEEGE 126
            AL+ LC+F+YF+LFLD+V ML+R ++ LD++ +E  E +++I +++ +     + E GE
Sbjct: 65  LALSLLCSFIYFLLFLDAVAMLFRTQYQLDIICKELKELFQQILVSKNQDDVGLSMETGE 124

Query: 127 DDSSHEVELIREMILTNSNYTRSSRRGMERKVYIYSISGALVGVAATELY 170
                E     +M++ +        R + RKVYIY    AL+ V A ELY
Sbjct: 125 SSGGFEFGFHEKMLMLD------HFRIVGRKVYIYFTVSALLAVTAIELY 168

BLAST of Sgr021188 vs. ExPASy TrEMBL
Match: A0A6J1DS87 (uncharacterized protein LOC111023927 OS=Momordica charantia OX=3673 GN=LOC111023927 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 8.1e-22
Identity = 68/175 (38.86%), Postives = 104/175 (59.43%), Query Frame = 0

Query: 5   FREVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWARFFFFAISRSS---LTCNGW 64
           F E++R FE L  + EK E+R++Y+E++ QNI +AY +W R FFFAIS++S   L C  W
Sbjct: 3   FGELKRNFEALKDLVEKQESRVQYHESRAQNITMAYLIWGRLFFFAISQTSSSLLKCIDW 62

Query: 65  WVTFALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREE------AN 124
           W+   L+  C FVYF+ FL++V MLYR +H +D++ +EQAE  ++I + R +      A 
Sbjct: 63  WMVLGLSVSCAFVYFLFFLEAVTMLYRVQHQMDIICKEQAEICQQILVARSQLDDVDLAM 122

Query: 125 EEGEDDSSHEVELIREMILTNSNYTRSSRRGMERKVYIYSISGALVGVAATELYA 171
           E G+     +     +++         + R +ERK YI +   AL+ V A ELYA
Sbjct: 123 EAGDSSDGFQFSFHVKLL------EYGAFRIVERKFYICATVSALLAVTAIELYA 171

BLAST of Sgr021188 vs. ExPASy TrEMBL
Match: A0A6J1DTV6 (uncharacterized protein LOC111023951 OS=Momordica charantia OX=3673 GN=LOC111023951 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.0e-20
Identity = 68/174 (39.08%), Postives = 106/174 (60.92%), Query Frame = 0

Query: 6   REVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWARFFFFAISRSS--LTCNGWWV 65
           R ++R FE+L  + EK E  LR+YE++ QNI + Y +W RFFFFA+S++S  L C  W V
Sbjct: 4   RVLKRDFEELKLLAEKQEPILRFYESRAQNITMGYLIWERFFFFALSQTSSPLKCIDWRV 63

Query: 66  TFALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREEANEEGEDDSS 125
              L+ +C+FVYF+LFL++V MLYR ++ +DM+ +EQ++    IC    +A  + +  S+
Sbjct: 64  VLGLSLVCSFVYFLLFLEAVTMLYRTQNQMDMICKEQSD----ICQQILDAGNQDDGGSA 123

Query: 126 HEVELIREMILTNSNYTRS-------SRRGMERKVYIYSISGALVGVAATELYA 171
            E   + + +  N ++          S R  ++KVYI +I  +LV V A ELYA
Sbjct: 124 MEAGDLSDGVAFNFSFHMRTTLNYDYSFRIFQKKVYISAIISSLVAVTALELYA 173

BLAST of Sgr021188 vs. ExPASy TrEMBL
Match: A0A0A0L822 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G484330 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 9.9e-20
Identity = 74/173 (42.77%), Postives = 102/173 (58.96%), Query Frame = 0

Query: 7   EVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWARFFFFAISR--------SSLTC 66
           E+E+R E+L AM EK EA   YY+TK+  II AYF+W R F FAIS         SSL C
Sbjct: 5   ELEKRMEELKAMSEKQEATANYYDTKLHTIIAAYFIWERAFCFAISNKTNSPNYFSSLIC 64

Query: 67  N-GWWVTFALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREEANEE 126
           +  W +  AL+SL + VY +L+LD+ LMLYR+E   +++  + A+ Y +I   ++E N  
Sbjct: 65  HANWRLILALSSLYSLVYILLYLDAALMLYRSELKQNLILNKHAQLYHQISKIKQEFNSI 124

Query: 127 GEDDSSHEVELIREMILTNSNYTRSSRRGMERKVYIYSISGALVGVAATELYA 171
                  E +LI   +L NS+ T   RR  ER  Y+ +I  ALV VA+ ELYA
Sbjct: 125 DSSSMEAEEDLI---LLINSSST--FRRSEERIFYMSTIFCALVCVASLELYA 172

BLAST of Sgr021188 vs. ExPASy TrEMBL
Match: A0A6J1DX74 (uncharacterized protein LOC111023953 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023953 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 3.2e-18
Identity = 66/169 (39.05%), Postives = 98/169 (57.99%), Query Frame = 0

Query: 7   EVERRFEQLNAMDEKLEARLRYYETKVQNIIIAYFVWARFFFFAISR-SSLTCNGWWVTF 66
           E+ R+F +L  ++EK E+R+RY+E K Q I+  Y +  R FFF IS+ SS  C+ WWV  
Sbjct: 5   ELRRKFGELKDINEKQESRVRYHEAKFQKIVSGYLILTRLFFFGISQTSSSKCHDWWVIL 64

Query: 67  ALNSLCTFVYFVLFLDSVLMLYRAEHHLDMMYQEQAEFYRKICMTREE-----ANEEGED 126
           +L+ LC+FVYF+LFLD+   LY+ +  LDM+ +E  E  ++I + + +     A E G+ 
Sbjct: 65  SLSLLCSFVYFLLFLDAATRLYQTKGQLDMICKELIEVCQQILVAQNQDDVDLAMEGGDF 124

Query: 127 DSSHEVELIREMILTNSNYTRSSRRGMERKVYIYSISGALVGVAATELY 170
               E     +M++ +        R + RKVYIY    ALV V A ELY
Sbjct: 125 SDGFEFGFHEKMLVLD------HFRFVGRKVYIYFTVCALVAVTAIELY 167