Sgr021184 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr021184 vs. NCBI nr
Match: KAG6579345.1 (hypothetical protein SDJN03_23793, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 141.0 bits (354), Expect = 1.4e-29
Identity = 80/183 (43.72%), Postives = 120/183 (65.57%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRLFFFAISHASPLPSSSSLSCKD 60
           +++ M+    +GE  E +V+YY TK+HNII+AY +W R+FFF IS  +  P+ S+LSC  
Sbjct: 10  LKKMMEEVQGMGENVEAKVNYYDTKLHNIIVAYFVWERVFFFGISKKTFPPTISTLSCNG 69

Query: 61  R-WVVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQICEARKNE----A 120
             WV+LA S  C  VY+LLF  T++MLYR E ++ +I QK  +L + +   ++ +    A
Sbjct: 70  NWWVILALSSSCSFVYVLLFFDTALMLYRHENQLHLILQKHAQLCRHLLAIKEEQADIKA 129

Query: 121 NMMEA-EDSSHGILIEER-MLTNSSGR-------GRKVYIYSTFCALVGVGGMELYACKF 170
           ++MEA +++SHG+ +EE  ML NS+          RKVY+Y+ FCAL+GV  +ELYACK 
Sbjct: 130 SLMEAGDEASHGLSLEEELMLINSTSPFRRRRPWERKVYVYTIFCALIGVASLELYACKA 189

BLAST of Sgr021184 vs. NCBI nr
Match: KAG6579344.1 (hypothetical protein SDJN03_23792, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-28
Identity = 79/184 (42.93%), Postives = 121/184 (65.76%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRL-----FFFAISHASPLPSSSS 60
           ME+ M+    + EK E RV+YY TK+H II+AYL+W R+     FFF +S+ +   + S+
Sbjct: 7   MEKRMEEVKGMWEKVEGRVNYYDTKLHAIIVAYLVWERVFFFFFFFFGVSNTNFASNIST 66

Query: 61  LSCKDR-WVVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQICEARKNE 120
           LSC  + WV++A SCLC  VYMLLF+  ++MLY  + ++++I Q  ++LY+Q+   +   
Sbjct: 67  LSCNGKWWVIVALSCLCSFVYMLLFVDAALMLYPHQNQLNLILQTHHQLYRQLLAIKD-- 126

Query: 121 ANMMEA-EDSSHGILIEER-MLTNSSGR------GRKVYIYSTFCALVGVGGMELYACKF 171
            ++MEA +++SHG+ +EE  ML NS+        GRK Y+Y+ FCAL+ V  +ELYAC  
Sbjct: 127 -SLMEAGDEASHGLSLEEELMLINSNAAYRRRPWGRKFYVYTIFCALIDVASLELYACNS 186

BLAST of Sgr021184 vs. NCBI nr
Match: KAG6579352.1 (hypothetical protein SDJN03_23800, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 136.0 bits (341), Expect = 4.5e-28
Identity = 79/183 (43.17%), Postives = 116/183 (63.39%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRLFFFAISHASPLPSSSSLSCKD 60
           +++ M+    +GEK E +V+YY TK+H II+AY +W R+FFF IS  +   + S+LSC  
Sbjct: 6   LKKMMEEVQGMGEKVEAKVNYYDTKLHTIIVAYFVWERVFFFGISKKTFPSTISTLSCNG 65

Query: 61  R-WVVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQIC----EARKNEA 120
             WV+LA SC C  VY+LLF   ++MLYR E ++ +I QK  +L + +     E    +A
Sbjct: 66  NWWVILALSCSCSFVYVLLFFDAALMLYRHENQLHLILQKHAQLCRHLLAIKEEQADTKA 125

Query: 121 NMMEAED-SSHGILIEER-MLTNSSGR-------GRKVYIYSTFCALVGVGGMELYACKF 170
           ++MEA D +SHG+ +EE  ML NS+          RKV++Y+ FCA +GV  +ELYACK 
Sbjct: 126 SLMEAGDQASHGLSLEEELMLINSTSPFRRRRPWERKVHVYTIFCAFIGVASLELYACKA 185

BLAST of Sgr021184 vs. NCBI nr
Match: XP_022157182.1 (uncharacterized protein LOC111023958 [Momordica charantia] >XP_022157183.1 uncharacterized protein LOC111023958 [Momordica charantia])

HSP 1 Score: 134.0 bits (336), Expect = 1.7e-27
Identity = 82/176 (46.59%), Postives = 110/176 (62.50%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRLFFFAISHASPLPSSSSLSCKD 60
           +ER+ +    I EKQE+RV YY+TKV NI+  YLI+ RLFFF IS      +SSS +CKD
Sbjct: 6   LERKFEELKDINEKQESRVRYYETKVQNIVFGYLIFTRLFFFGISQ-----TSSSFNCKD 65

Query: 61  RWVVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQICEARKNE--ANMM 120
            WV+LA S LC  +Y LLFL    ML+R + ++DIIC++  EL+QQI  ++  +     M
Sbjct: 66  WWVILALSLLCSFIYFLLFLDAVAMLFRTQYQLDIICKELKELFQQILVSKNQDDVGLSM 125

Query: 121 EAEDSSHGIL--IEERMLTNSSGR--GRKVYIYSTFCALVGVGGMELYACKFLLCS 171
           E  +SS G      E+ML     R  GRKVYIY T  AL+ V  +ELY  K++LC+
Sbjct: 126 ETGESSGGFEFGFHEKMLMLDHFRIVGRKVYIYFTVSALLAVTAIELYVSKYVLCN 176

BLAST of Sgr021184 vs. NCBI nr
Match: XP_022157130.1 (uncharacterized protein LOC111023927 [Momordica charantia])

HSP 1 Score: 119.4 bits (298), Expect = 4.3e-23
Identity = 78/176 (44.32%), Postives = 104/176 (59.09%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRLFFFAISHASPLPSSSSLSCKD 60
           ++R  +    + EKQE+RV Y++++  NI +AYLIW RLFFFAIS      SSS L C D
Sbjct: 6   LKRNFEALKDLVEKQESRVQYHESRAQNITMAYLIWGRLFFFAISQT----SSSLLKCID 65

Query: 61  RWVVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQICEARKNEANM--- 120
            W+VL  S  C  VY L FL    MLYR + ++DIIC++Q E+ QQI  AR    ++   
Sbjct: 66  WWMVLGLSVSCAFVYFLFFLEAVTMLYRVQHQMDIICKEQAEICQQILVARSQLDDVDLA 125

Query: 121 MEAEDSSHG--ILIEERMLTNSSGR--GRKVYIYSTFCALVGVGGMELYACKFLLC 170
           MEA DSS G       ++L   + R   RK YI +T  AL+ V  +ELYAC +L C
Sbjct: 126 MEAGDSSDGFQFSFHVKLLEYGAFRIVERKFYICATVSALLAVTAIELYACSWLYC 177

BLAST of Sgr021184 vs. ExPASy TrEMBL
Match: A0A6J1DSQ0 (uncharacterized protein LOC111023958 OS=Momordica charantia OX=3673 GN=LOC111023958 PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 8.2e-28
Identity = 82/176 (46.59%), Postives = 110/176 (62.50%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRLFFFAISHASPLPSSSSLSCKD 60
           +ER+ +    I EKQE+RV YY+TKV NI+  YLI+ RLFFF IS      +SSS +CKD
Sbjct: 6   LERKFEELKDINEKQESRVRYYETKVQNIVFGYLIFTRLFFFGISQ-----TSSSFNCKD 65

Query: 61  RWVVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQICEARKNE--ANMM 120
            WV+LA S LC  +Y LLFL    ML+R + ++DIIC++  EL+QQI  ++  +     M
Sbjct: 66  WWVILALSLLCSFIYFLLFLDAVAMLFRTQYQLDIICKELKELFQQILVSKNQDDVGLSM 125

Query: 121 EAEDSSHGIL--IEERMLTNSSGR--GRKVYIYSTFCALVGVGGMELYACKFLLCS 171
           E  +SS G      E+ML     R  GRKVYIY T  AL+ V  +ELY  K++LC+
Sbjct: 126 ETGESSGGFEFGFHEKMLMLDHFRIVGRKVYIYFTVSALLAVTAIELYVSKYVLCN 176

BLAST of Sgr021184 vs. ExPASy TrEMBL
Match: A0A6J1DS87 (uncharacterized protein LOC111023927 OS=Momordica charantia OX=3673 GN=LOC111023927 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 2.1e-23
Identity = 78/176 (44.32%), Postives = 104/176 (59.09%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRLFFFAISHASPLPSSSSLSCKD 60
           ++R  +    + EKQE+RV Y++++  NI +AYLIW RLFFFAIS      SSS L C D
Sbjct: 6   LKRNFEALKDLVEKQESRVQYHESRAQNITMAYLIWGRLFFFAISQT----SSSLLKCID 65

Query: 61  RWVVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQICEARKNEANM--- 120
            W+VL  S  C  VY L FL    MLYR + ++DIIC++Q E+ QQI  AR    ++   
Sbjct: 66  WWMVLGLSVSCAFVYFLFFLEAVTMLYRVQHQMDIICKEQAEICQQILVARSQLDDVDLA 125

Query: 121 MEAEDSSHG--ILIEERMLTNSSGR--GRKVYIYSTFCALVGVGGMELYACKFLLC 170
           MEA DSS G       ++L   + R   RK YI +T  AL+ V  +ELYAC +L C
Sbjct: 126 MEAGDSSDGFQFSFHVKLLEYGAFRIVERKFYICATVSALLAVTAIELYACSWLYC 177

BLAST of Sgr021184 vs. ExPASy TrEMBL
Match: A0A0A0L822 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G484330 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 6.1e-23
Identity = 75/176 (42.61%), Postives = 107/176 (60.80%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRLFFFAISHASPLPS-SSSLSCK 60
           +E+ M+   A+ EKQE   +YY TK+H II AY IW R F FAIS+ +  P+  SSL C 
Sbjct: 6   LEKRMEELKAMSEKQEATANYYDTKLHTIIAAYFIWERAFCFAISNKTNSPNYFSSLICH 65

Query: 61  DRW-VVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQICEARKN----E 120
             W ++LA S L  LVY+LL+L  ++MLYR+E + ++I  K  +LY QI + ++     +
Sbjct: 66  ANWRLILALSSLYSLVYILLYLDAALMLYRSELKQNLILNKHAQLYHQISKIKQEFNSID 125

Query: 121 ANMMEAEDSSHGILIEERMLTNSSGRGRKVYIYSTFCALVGVGGMELYACKFLLCS 171
           ++ MEAE+    IL+     T      R  Y+ + FCALV V  +ELYACK +LCS
Sbjct: 126 SSSMEAEEDL--ILLINSSSTFRRSEERIFYMSTIFCALVCVASLELYACKSILCS 179

BLAST of Sgr021184 vs. ExPASy TrEMBL
Match: A0A6J1DX74 (uncharacterized protein LOC111023953 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023953 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.1e-21
Identity = 76/176 (43.18%), Postives = 100/176 (56.82%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRLFFFAISHASPLPSSSSLSCKD 60
           + R+      I EKQE+RV Y++ K   I+  YLI  RLFFF IS       +SS  C D
Sbjct: 6   LRRKFGELKDINEKQESRVRYHEAKFQKIVSGYLILTRLFFFGIS------QTSSSKCHD 65

Query: 61  RWVVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQICEARKNE--ANMM 120
            WV+L+ S LC  VY LLFL  +  LY+ + ++D+IC++  E+ QQI  A+  +     M
Sbjct: 66  WWVILSLSLLCSFVYFLLFLDAATRLYQTKGQLDMICKELIEVCQQILVAQNQDDVDLAM 125

Query: 121 EAEDSSHGIL--IEERMLTNSSGR--GRKVYIYSTFCALVGVGGMELYACKFLLCS 171
           E  D S G      E+ML     R  GRKVYIY T CALV V  +ELY  K+LLC+
Sbjct: 126 EGGDFSDGFEFGFHEKMLVLDHFRFVGRKVYIYFTVCALVAVTAIELYVSKYLLCN 175

BLAST of Sgr021184 vs. ExPASy TrEMBL
Match: A0A0A0KUT0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G562290 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 2.0e-21
Identity = 64/175 (36.57%), Postives = 106/175 (60.57%), Query Frame = 0

Query: 1   MEREMDMWNAIGEKQETRVHYYQTKVHNIIIAYLIWVRLFFFAISHASPLPSSSSLSCKD 60
           M++ ++  NA+ + Q+ RV  Y+T + NI IA+L+W+RLFFF++S  S  P+SS L CK 
Sbjct: 9   MQKVLEELNAMADTQKGRVENYETSLQNIAIAFLVWLRLFFFSLSQTS--PNSSLLHCKH 68

Query: 61  RWVVLAFSCLCILVYMLLFLYTSMMLYRAEQRVDIICQKQNELYQQICEARKNEANMMEA 120
            W++ A +C    +Y+LLF++ S+ML R E+++ +I ++Q +L+QQI   R  E   M  
Sbjct: 69  WWLLFALTCFSAFLYILLFIHNSLMLSRTERQLHVISRQQIQLHQQIWMLRLQELPQMIE 128

Query: 121 EDSSHGILIEERMLTNSSGR----GRKVYIYSTFCALVGVGGMELYACKFLLCSS 172
                  +I + ++    GR     RK+YI    C  +G+  +ELYA + LLC++
Sbjct: 129 P------IIMDHIINGEMGRTSTFERKLYINCILCGFIGIVALELYASRSLLCNA 175