Sgr021073 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr021073
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	delta(7)-sterol-C5(6)-desaturase-like
Location	tig00153640: 259280 .. 263251 (-)
RNA-Seq Expression	Sgr021073
Synteny	Sgr021073

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAGCGAAGTGAGATGGAGGGTCGGGTCTGGTCTGGTCTGGTCTGGCTGGTCCGGTCCTACCAGCGGCAGAGTGTAGAGCGGGGTGGTCCTTCTCTTCCTCCTCGATGTAGTAGCAGAGCACTTTGTCGATTATTTAAGCCTCCCCCGCAGATAAAAATGCTCACGACCCTCTTCGATTTCCAACTCAGACTCTCTCTTTCTTTCCTTCTTTTTCTGTTTGAAGTTGCGCTGGCTTTGATTGACTGCTTTTTCCTCCTTTTTCCGGCGGATGGAGTGGGGGAATTCGACTGCCATGGCCGGCGGGCCGTCGTATCTGCAGCTCTTTGTGGAGGAGACCAGTTTCTACAACCGCATTGTTCTCGGTGGCATTTTGCGGGCCGTTGCCTTGGACCAACTGCCTCATATTATTCAGACATGGCTTCGCAACGTCATTGGTGGATTCGTAATCTACTTCTTCTCTTCCTTCCTTTGGTGCTTCTACATCTATTACTGGAAGCACAATATTTATGTCCCAAAAGGTGTTTGCCTTCTTCTTCTTCTTCTTCTTCTCCTTCTCTCTCTTTACTCCCCACTTTTTTCCTCTTTTGTTACTCTCTCAACTTTCCCATTAATGAGTTCCAATTCTCGTTATTTGGGTTCAGTATTTCACTTGGTTCTTTGTTGGGTCCCTATTCTTTTTCTTCTTATTTGATCTTCTTAATATATCACTAATTATACAATGAATTGTATGTCATTGGGAAAATCCTGGTTGTGTGTTTCTTTTACTAATTTGGCCACAACAGCCAGATTGATAGAGCTGTATTCTTGAACTTTTTTTTTTTCTTTTTTCTTTTACGTGTGCATGCTATATCTAATTCATTTTGGTTCTACTTCTTAATTTGTTATAGAAAGTTGAGATGTTTTATTTGATTCTCATTGATTGACTCTTCTGCTGCCATTGGATGTGCTGAAACAATTTGTCTGGAAGTCTCTTATTGAGATAAACTTGATATGTCTTTGACGAGGTTGTTTCTGCCTTCTGAGAGGCTGATGTTTCTTGTCCAATCCAGGATGGACTATTATAACTGCTTACTAGCCACAATATTTTTGAATCATCCATAACTTCTTGTCAGATAGACTTCTCAAAAAGGCTTTTTCATTTGTTCAAGCTGATGATTCTTTTTTCCTGTCTTGTCCTAGATGAATTGTAATGAAAAGAACTGTAACAACCAACATATAACCATAATACTTTAGTACTAATCGATGGTATGTTCTGTTGAGATTTTTATCTATAGGCTCTTCTCAGTTAGACTACTTAAAGAGGGTGTTTTATTTGTTCAAATGGATGATTCTTCTATGCATGAACATATATATATATATATATATATATAAAGAAAGGACATTTAAAAGGTTAGAATAGTAAGGACTTGTAGAATAGTAAGGACTTGTTAATATTTAGTTCAATCTTTACCTTTCTCATGGCCCTATATAGTTATTGGTATAAGTAATATCTTTGTTGTAATATTACATAGATCTCATAAACTTTTTGCCAGCCTATTTCATAGTTTCCTTTTGATAAGCTTTTTTTTTCTTATTATTTCATTTGAACAAACATGCACATAGTATTTCCTTCAAGAACCTTTGTTAGTCTTGGCAAACAAGATTAGTCTTAGGTTGAGTTTTAGATGACTCTACCCAGTAAATGTTCTATACTGAGCATTGGCTTGTTTGTGGGATTGTAAATCAACTATATGCTCTTTTAGATATCCATATACACACATGATGTTTGTGATTTAGAATTTTTTTTTTAATATGTGGTCTCTGGGCAACCTTGACTAATTTCACAAAACAATTGCCTATTTTTATACATTTGGTGTCAAGAAACTTGTGGGATATTAAGATTGTATGTAGGTGGCTATTTTGAACTAAATATTAATTCAACCCTATTTTCGAACATTGAACTTGTTAGAATCGGCACGCTATTAACTTTTTTCCTATTTGTTACTTTTGTATGAATATTATATGCCATTTTTTGTTAGCATTATGTTTGGATATAGATTGTAGTAATGATGCACTTGGTAATTGTCGTTTGGGGACGAACCATTTAATCTGATAAGCTTTAAAACTCAGACATTCTGTTGATCATCTGCCTCTAATTTGCAATATGATGATTTGATTACTTCTCCCCCATAATTCGTCAGCAGTTTAAGACGTCGAGCTTTTGAAATATTATTCGAAAAAAAACATGCATCTTAATATATAAAAGTCGTCATTTATGGAGTATATTCTGGATTTGGATGAGACTGTTATGTAAGAATCCAAGTAACTCTTTTAGACTTGCAATATCCTTCATGTCGTAACGACATTATAAGTTACTGAGTGTGTATGGCTTTTGATGCTTGTATAATTATTTGACCTACTGGACACTTGATTGCAGATTCTATCCCGTCTGGTAAAGCAGTGCTCTTGCAAATCGGGGTAACAGCCAAGGCCATGCCATTGTACTGCACCCTTCCAACGTTTTCTGAATATGTGGTTGAACATGACTGGACAAAATGCTATCCAAGAGTACACGATGTCGGATGGCTTGCATATGGCTTCCATATAATTGCTTATCTTACCTTCATAGAGTTTTTTATTTATTGGATGCATAGGGGGCTGCATGATATAAAGCCTCTTTACAAGTATCTTCATGCTAAACATCATATGTTCAACAAGAAAGTTACTCTCTCTCCCTTTGCTGGTAATTTCTTAACCTACTATCTCAATTTTTGCAACCACTTGCTATTCATGTCAAAGAAACTTCTTTGAAAAGATTGTATGTCATATGCCATTCTTGTTTGATTTCCTTAAGAAATGTTCAACTTTATCTGAGAAACTGATAAAAACCGATCTCGTCTGAACTATTTCCCAAAATTTCTTTTACAAAAAAACTGACCCATATTTACAGAAAATTGAAAAAGTCTACTTAGCATTAAGCTGCAGTGTAAACTGTTAGGACAGTAGCAAGTATTAAGACTAAAGGTACTAGAGTACTTGTTTCCTTAAAATGAATTTCTGATCTTTGCTGATTGGAGAACAATTTAGCCTAGCGTGAACGTTAAGAGTGGTGAGGAGTCATTGCTTCCGTAGGACTTACATACTAAGTCACATGAACCTGAACTTTTGGCATGCTTTTATGTATCATTTTGGCATACTATATCTGTGCAAAAGTCTTCTTTTTTATCGACTAAATAGAGGTCTTTTGAGCTGGCTAAGACTATAAATTATTTCTGTAAAATGGAAATCTTTAAAATCCCAAAATACACTGATGTTAGTGATGATTGCATTCTGAAGTAGTTGGGACATTGATCTATCAAGTTGCATAATTTCCTCTCTCCTTCTCGTGACTTCAGACTTGAGAAGTTGTTTGGTTTGTTTCTCATTATTAGAAAATCATCTGATAGTAGTCTCTTATTATGTCAGGTCTCGCATTTCACCCCGTCGATGGGATATTACAGGCAATACCTCATCTGTTTGCTCTATTTTTCATTCCAACCCATTTTAGAACACATATTGTGCTGCTATTCCTCGAGGTGATATGGACGACGAACATTCACGATTGCATCCATTCGCACTTGTGGCCAGTTATGGGAGCTGGATATCACACCATTCATCATACAAGATACCGCTACAATTATGGCCATTACACCATATGGATGGATTGGATGTTCGGGACGCTTCTTGACCCAATGGATACAAAAGCTTAGATGTTGTGATCGGGTTGCTTTGACGTCAACTGCTTAGTCATTGGTGATTCTTTGTGTTCTTAATGATATATTGATCTTCGAATGTAAATCAAGCTGGTTTTTGATGAGGCCATGTCTTCTAGTATGTTATAGAATAGTTGCCACTGCAGGGCACTCGGTGCCATCTATACATCTACATTCAATCTCACAGAGTTTAGGAATCCACAGGGCCTGTGTGCTGCTGCTGCTGCTATTATTG

mRNA sequence

ATGGGAAAGCGAAGTGAGATGGAGGGTCGGGTCTGGTCTGGTCTGGTCTGGCTGGTCCGGTCCTACCAGCGGCAGAGTGTAGAGCGGGGTGGTCCTTCTCTTCCTCCTCGATGTAGTAGCAGAGCACTTTGTCGATTATTTAAGCCTCCCCCGCAGATAAAAATGCTCACGACCCTCTTCGATTTCCAACTCAGACTCTCTCTTTCTTTCCTTCTTTTTCTGTTTGAAGTTGCGCTGGCTTTGATTGACTGCTTTTTCCTCCTTTTTCCGGCGGATGGAGTGGGGGAATTCGACTGCCATGGCCGGCGGGCCGTCGTATCTGCAGCTCTTTGTGGAGGAGACCAGTTTCTACAACCGCATTGTTCTCGGTGGCATTTTGCGGGCCGTTGCCTTGGACCAACTGCCTCATATTATTCAGACATGGCTTCGCAACATTCTATCCCGTCTGGTAAAGCAGTGCTCTTGCAAATCGGGGTAACAGCCAAGGCCATGCCATTGTACTGCACCCTTCCAACGTTTTCTGAATATGTGGTTGAACATGACTGGACAAAATGCTATCCAAGAGTACACGATGTCGGATGGCTTGCATATGGCTTCCATATAATTGCTTATCTTACCTTCATAGAGTTTTTTATTTATTGGATGCATAGGGGGCTGCATGATATAAAGCCTCTTTACAAGTATCTTCATGCTAAACATCATATGTTCAACAAGAAAGTTACTCTCTCTCCCTTTGCTGGTCTCGCATTTCACCCCGTCGATGGGATATTACAGGCAATACCTCATCTGTTTGCTCTATTTTTCATTCCAACCCATTTTAGAACACATATTGTGCTGCTATTCCTCGAGAATAGTTGCCACTGCAGGGCACTCGGTGCCATCTATACATCTACATTCAATCTCACAGAGTTTAGGAATCCACAGGGCCTGTGTGCTGCTGCTGCTGCTATTATTG

Coding sequence (CDS)

ATGGGAAAGCGAAGTGAGATGGAGGGTCGGGTCTGGTCTGGTCTGGTCTGGCTGGTCCGGTCCTACCAGCGGCAGAGTGTAGAGCGGGGTGGTCCTTCTCTTCCTCCTCGATGTAGTAGCAGAGCACTTTGTCGATTATTTAAGCCTCCCCCGCAGATAAAAATGCTCACGACCCTCTTCGATTTCCAACTCAGACTCTCTCTTTCTTTCCTTCTTTTTCTGTTTGAAGTTGCGCTGGCTTTGATTGACTGCTTTTTCCTCCTTTTTCCGGCGGATGGAGTGGGGGAATTCGACTGCCATGGCCGGCGGGCCGTCGTATCTGCAGCTCTTTGTGGAGGAGACCAGTTTCTACAACCGCATTGTTCTCGGTGGCATTTTGCGGGCCGTTGCCTTGGACCAACTGCCTCATATTATTCAGACATGGCTTCGCAACATTCTATCCCGTCTGGTAAAGCAGTGCTCTTGCAAATCGGGGTAACAGCCAAGGCCATGCCATTGTACTGCACCCTTCCAACGTTTTCTGAATATGTGGTTGAACATGACTGGACAAAATGCTATCCAAGAGTACACGATGTCGGATGGCTTGCATATGGCTTCCATATAATTGCTTATCTTACCTTCATAGAGTTTTTTATTTATTGGATGCATAGGGGGCTGCATGATATAAAGCCTCTTTACAAGTATCTTCATGCTAAACATCATATGTTCAACAAGAAAGTTACTCTCTCTCCCTTTGCTGGTCTCGCATTTCACCCCGTCGATGGGATATTACAGGCAATACCTCATCTGTTTGCTCTATTTTTCATTCCAACCCATTTTAGAACACATATTGTGCTGCTATTCCTCGAGAATAGTTGCCACTGCAGGGCACTCGGTGCCATCTATACATCTACATTCAATCTCACAGAGTTTAGGAATCCACAGGGCCTGTGTGCTGCTGCTGCTGCTATTATTG

Protein sequence

MGKRSEMEGRVWSGLVWLVRSYQRQSVERGGPSLPPRCSSRALCRLFKPPPQIKMLTTLFDFQLRLSLSFLLFLFEVALALIDCFFLLFPADGVGEFDCHGRRAVVSAALCGGDQFLQPHCSRWHFAGRCLGPTASYYSDMASQHSIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYLTFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFALFFIPTHFRTHIVLLFLENSCHCRALGAIYTSTFNLTEFRNPQGLCAAAAAIIX

Homology

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BLAST of Sgr021073 vs. NCBI nr
Match: XP_022148152.1 (delta(7)-sterol-C5(6)-desaturase-like isoform X2 [Momordica charantia])

HSP 1 Score: 261.9 bits (668), Expect = 7.1e-66
Identity = 120/139 (86.33%), Postives = 129/139 (92.81%), Query Frame = 0


Query: 145 HSIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAY 204
           HSIPS K++LLQI VT  AMPLYC LPTFSEYVVEHDWTKCYPR+ DVGWLAY FHII+Y
Sbjct: 3   HSIPSNKSMLLQIYVTITAMPLYCILPTFSEYVVEHDWTKCYPRIRDVGWLAYAFHIISY 62

Query: 205 LTFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLF 264
           LTF+EF IYWMHRGLHDIKPLYKYLHAKHH++NKK TLSPFAGLAFHP+DGILQAIPHLF
Sbjct: 63  LTFVEFSIYWMHRGLHDIKPLYKYLHAKHHIYNKKSTLSPFAGLAFHPIDGILQAIPHLF 122

Query: 265 ALFFIPTHFRTHIVLLFLE 284
           ALF IPTHFRTH+VLLFLE
Sbjct: 123 ALFLIPTHFRTHVVLLFLE 141

[Close]

BLAST of Sgr021073 vs. NCBI nr
Match: XP_022148151.1 (delta(7)-sterol-C5(6)-desaturase-like isoform X1 [Momordica charantia])

HSP 1 Score: 258.5 bits (659), Expect = 7.8e-65
Identity = 119/138 (86.23%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           SIPS K++LLQI VT  AMPLYC LPTFSEYVVEHDWTKCYPR+ DVGWLAY FHII+YL
Sbjct: 77  SIPSNKSMLLQIYVTITAMPLYCILPTFSEYVVEHDWTKCYPRIRDVGWLAYAFHIISYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
           TF+EF IYWMHRGLHDIKPLYKYLHAKHH++NKK TLSPFAGLAFHP+DGILQAIPHLFA
Sbjct: 137 TFVEFSIYWMHRGLHDIKPLYKYLHAKHHIYNKKSTLSPFAGLAFHPIDGILQAIPHLFA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF IPTHFRTH+VLLFLE
Sbjct: 197 LFLIPTHFRTHVVLLFLE 214

[Close]

BLAST of Sgr021073 vs. NCBI nr
Match: XP_004150515.1 (delta(7)-sterol-C5(6)-desaturase [Cucumis sativus] >KGN54626.1 hypothetical protein Csa_012304 [Cucumis sativus])

HSP 1 Score: 256.1 bits (653), Expect = 3.9e-64
Identity = 120/138 (86.96%), Postives = 127/138 (92.03%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           SIPS K++LLQI VT KAMPLYC LPTF+EYVVEHDWTKCYPRV DVGW AY FHII YL
Sbjct: 77  SIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRVLDVGWPAYAFHIITYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
           TFIEF IYWMHRGLHDIKPLYKYLHAKHH++NKK+TLSPFAGLAFHP+DGILQAIPHLFA
Sbjct: 137 TFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPMDGILQAIPHLFA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF IPTHFRTHIVLLF E
Sbjct: 197 LFLIPTHFRTHIVLLFFE 214

[Close]

BLAST of Sgr021073 vs. NCBI nr
Match: XP_038894105.1 (delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894106.1 delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894107.1 delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida])

HSP 1 Score: 255.8 bits (652), Expect = 5.1e-64
Identity = 119/138 (86.23%), Postives = 126/138 (91.30%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           SIPS K++LLQI VT KAMPLYC LPTFSEYVVEHDWTKCYPRV DVGW AY FH+I YL
Sbjct: 77  SIPSNKSMLLQIFVTIKAMPLYCILPTFSEYVVEHDWTKCYPRVLDVGWPAYAFHVITYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
            FIEF IYWMHRGLHDIKPLYKYLHAKHH++NKK+TLSPFAGLAFHP+DGILQAIPHLFA
Sbjct: 137 AFIEFSIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPIDGILQAIPHLFA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF IPTHFRTHIVLLF E
Sbjct: 197 LFLIPTHFRTHIVLLFFE 214

[Close]

BLAST of Sgr021073 vs. NCBI nr
Match: XP_008444093.1 (PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo])

HSP 1 Score: 255.0 bits (650), Expect = 8.6e-64
Identity = 120/138 (86.96%), Postives = 126/138 (91.30%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           SIPS K++LLQI VT KAMPLYC LPTF+EYVVEHDWTKCYPRV DVGW AY FHII YL
Sbjct: 77  SIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRVLDVGWPAYAFHIITYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
           TFIEF IYWMHRGLHDIKPLYKYLHAKHH++NKK TLSPFAGLAFHP+DGILQAIPHLFA
Sbjct: 137 TFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKTTLSPFAGLAFHPMDGILQAIPHLFA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF IPTHFRTHIVLLF E
Sbjct: 197 LFLIPTHFRTHIVLLFFE 214

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BLAST of Sgr021073 vs. ExPASy Swiss-Prot
Match: Q9ZT29 (Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 8.7e-51
Identity = 91/138 (65.94%), Postives = 110/138 (79.71%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           +IPS KA+LLQI V  KAMP YC LP+ SEY++E+ WTKC+ R+ DVGWL+Y  +   YL
Sbjct: 74  AIPSNKAMLLQISVAMKAMPWYCALPSLSEYMIENGWTKCFARISDVGWLSYVIYAAIYL 133

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
             +EF IYWMH  LHDIKPLYKYLHA HH++NK+ TLSPFAGLAFHP+DGILQA+PH+ A
Sbjct: 134 VIVEFGIYWMHMELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDGILQAVPHVVA 193

Query: 266 LFFIPTHFRTHIVLLFLE 284
           L  +P HF THI L+FLE
Sbjct: 194 LLLVPMHFSTHIALIFLE 211

[Close]

BLAST of Sgr021073 vs. ExPASy Swiss-Prot
Match: Q39208 (Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=STE1 PE=1 SV=2)

HSP 1 Score: 189.1 bits (479), Expect = 7.6e-47
Identity = 88/138 (63.77%), Postives = 107/138 (77.54%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           +IP+ KA+ LQ+ V  KAMP Y  LPT SE ++E  WTKC+  + + GW+ Y  +I  YL
Sbjct: 77  AIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASIGEFGWILYFVYIAIYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
            F+EF IYWMHR LHDIKPLYKYLHA HH++NK+ TLSPFAGLAFHPVDGILQA+PH+ A
Sbjct: 137 VFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPVDGILQAVPHVIA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF +P HF THI LLF+E
Sbjct: 197 LFIVPIHFTTHIGLLFME 214

[Close]

BLAST of Sgr021073 vs. ExPASy Swiss-Prot
Match: Q9M883 (Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=HDF7 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-44
Identity = 86/140 (61.43%), Postives = 103/140 (73.57%), Query Frame = 0


Query: 144 QHSIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIA 203
           + SIP+ KA+LLQI V  KAMP Y  LP  SEY++EH WTKCY  +    W     +I  
Sbjct: 76  KESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWFLCFLYIAL 135

Query: 204 YLTFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHL 263
           YL  +EF IYW+H+ LHDIK LYK+LHA HHM+NK+ TLSPFAGLAFHP+DGILQAIPH+
Sbjct: 136 YLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDGILQAIPHV 195

Query: 264 FALFFIPTHFRTHIVLLFLE 284
            ALF +P H  TH+ LLFLE
Sbjct: 196 IALFIVPIHLITHLSLLFLE 215

[Close]

BLAST of Sgr021073 vs. ExPASy Swiss-Prot
Match: Q9EQS5 (Lathosterol oxidase OS=Rattus norvegicus OX=10116 GN=Sc5d PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 7.0e-16
Identity = 54/156 (34.62%), Postives = 88/156 (56.41%), Query Frame = 0


Query: 156 QIGVTAKAMPLYCTLPTFSEYVVE-HDWTKCYPRVHDV--GWLAYGFHIIAYLTFIEFFI 215
           +I  T K++P + ++PT S +++E   ++K Y  + D   GW+     +I++L F +  I
Sbjct: 76  EIMFTVKSLP-WISIPTVSLFLLELRGYSKLYDDIGDFPNGWIHLIMSVISFLFFTDMLI 135

Query: 216 YWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFALFFIPTH 275
           YW+HRGLH  + LYK++H  HH++      +PFA  AFHPVDG LQ++P+    F  P H
Sbjct: 136 YWIHRGLHH-RLLYKHIHKPHHIWK---IPTPFASHAFHPVDGFLQSLPYHIYPFVFPLH 195

Query: 276 FRTHIVLLFLENSCHCRALGAIYTSTFNLTEFRNPQ 309
              ++ L  L N         ++T + +  +FR PQ
Sbjct: 196 KVVYLGLYVLVN---------VWTISIHDGDFRVPQ 217

[Close]

BLAST of Sgr021073 vs. ExPASy Swiss-Prot
Match: O88822 (Lathosterol oxidase OS=Mus musculus OX=10090 GN=Sc5d PE=2 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 5.9e-15
Identity = 52/156 (33.33%), Postives = 87/156 (55.77%), Query Frame = 0


Query: 156 QIGVTAKAMPLYCTLPTFSEYVVE-HDWTKCYPRVHDV--GWLAYGFHIIAYLTFIEFFI 215
           +I  T K++P + ++PT S +++E   ++K Y  + D   GW+     ++++L F +  I
Sbjct: 76  EIVFTVKSLP-WISIPTVSLFLLELRGYSKLYDDIGDFPNGWIHLMVSVVSFLFFTDMLI 135

Query: 216 YWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFALFFIPTH 275
           YW+HRGLH  + +YK +H  HH++      +PFA  AFHPVDG LQ++P+    F  P H
Sbjct: 136 YWIHRGLHH-RLVYKRIHKPHHIWK---IPTPFASHAFHPVDGFLQSLPYHIYPFVFPLH 195

Query: 276 FRTHIVLLFLENSCHCRALGAIYTSTFNLTEFRNPQ 309
              ++ L  L N         ++T + +  +FR PQ
Sbjct: 196 KVVYLGLYVLVN---------VWTISIHDGDFRVPQ 217

[Close]

BLAST of Sgr021073 vs. ExPASy TrEMBL
Match: A0A6J1D4A6 (delta(7)-sterol-C5(6)-desaturase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016898 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 3.4e-66
Identity = 120/139 (86.33%), Postives = 129/139 (92.81%), Query Frame = 0


Query: 145 HSIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAY 204
           HSIPS K++LLQI VT  AMPLYC LPTFSEYVVEHDWTKCYPR+ DVGWLAY FHII+Y
Sbjct: 3   HSIPSNKSMLLQIYVTITAMPLYCILPTFSEYVVEHDWTKCYPRIRDVGWLAYAFHIISY 62

Query: 205 LTFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLF 264
           LTF+EF IYWMHRGLHDIKPLYKYLHAKHH++NKK TLSPFAGLAFHP+DGILQAIPHLF
Sbjct: 63  LTFVEFSIYWMHRGLHDIKPLYKYLHAKHHIYNKKSTLSPFAGLAFHPIDGILQAIPHLF 122

Query: 265 ALFFIPTHFRTHIVLLFLE 284
           ALF IPTHFRTH+VLLFLE
Sbjct: 123 ALFLIPTHFRTHVVLLFLE 141

[Close]

BLAST of Sgr021073 vs. ExPASy TrEMBL
Match: A0A6J1D4I4 (delta(7)-sterol-C5(6)-desaturase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016898 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 3.8e-65
Identity = 119/138 (86.23%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           SIPS K++LLQI VT  AMPLYC LPTFSEYVVEHDWTKCYPR+ DVGWLAY FHII+YL
Sbjct: 77  SIPSNKSMLLQIYVTITAMPLYCILPTFSEYVVEHDWTKCYPRIRDVGWLAYAFHIISYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
           TF+EF IYWMHRGLHDIKPLYKYLHAKHH++NKK TLSPFAGLAFHP+DGILQAIPHLFA
Sbjct: 137 TFVEFSIYWMHRGLHDIKPLYKYLHAKHHIYNKKSTLSPFAGLAFHPIDGILQAIPHLFA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF IPTHFRTH+VLLFLE
Sbjct: 197 LFLIPTHFRTHVVLLFLE 214

[Close]

BLAST of Sgr021073 vs. ExPASy TrEMBL
Match: A0A0A0L3B9 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G409800 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.9e-64
Identity = 120/138 (86.96%), Postives = 127/138 (92.03%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           SIPS K++LLQI VT KAMPLYC LPTF+EYVVEHDWTKCYPRV DVGW AY FHII YL
Sbjct: 77  SIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRVLDVGWPAYAFHIITYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
           TFIEF IYWMHRGLHDIKPLYKYLHAKHH++NKK+TLSPFAGLAFHP+DGILQAIPHLFA
Sbjct: 137 TFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPMDGILQAIPHLFA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF IPTHFRTHIVLLF E
Sbjct: 197 LFLIPTHFRTHIVLLFFE 214

[Close]

BLAST of Sgr021073 vs. ExPASy TrEMBL
Match: A0A1S3BAC7 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucumis melo OX=3656 GN=LOC103487532 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 4.2e-64
Identity = 120/138 (86.96%), Postives = 126/138 (91.30%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           SIPS K++LLQI VT KAMPLYC LPTF+EYVVEHDWTKCYPRV DVGW AY FHII YL
Sbjct: 77  SIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRVLDVGWPAYAFHIITYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
           TFIEF IYWMHRGLHDIKPLYKYLHAKHH++NKK TLSPFAGLAFHP+DGILQAIPHLFA
Sbjct: 137 TFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKTTLSPFAGLAFHPMDGILQAIPHLFA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF IPTHFRTHIVLLF E
Sbjct: 197 LFLIPTHFRTHIVLLFFE 214

[Close]

BLAST of Sgr021073 vs. ExPASy TrEMBL
Match: A0A6J1KDR9 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC111492934 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 9.3e-64
Identity = 116/138 (84.06%), Postives = 123/138 (89.13%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           SIPS K +LLQI  T KAMPLYC LPTFSEYVVEHDWT+CYPRV DVGWLAY FH+  YL
Sbjct: 77  SIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWLAYAFHVTTYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
           TF+EFFIYWMHR LHD+KPLYKYLHAKHHM+NKK TLSPFAGLAFHPVDGILQAIPHLFA
Sbjct: 137 TFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDGILQAIPHLFA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF IP HFRTHI+LLF E
Sbjct: 197 LFLIPIHFRTHIMLLFFE 214

[Close]

BLAST of Sgr021073 vs. TAIR 10
Match: AT3G02580.1 (sterol 1 )

HSP 1 Score: 189.1 bits (479), Expect = 5.4e-48
Identity = 88/138 (63.77%), Postives = 107/138 (77.54%), Query Frame = 0


Query: 146 SIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIAYL 205
           +IP+ KA+ LQ+ V  KAMP Y  LPT SE ++E  WTKC+  + + GW+ Y  +I  YL
Sbjct: 77  AIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASIGEFGWILYFVYIAIYL 136

Query: 206 TFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHLFA 265
            F+EF IYWMHR LHDIKPLYKYLHA HH++NK+ TLSPFAGLAFHPVDGILQA+PH+ A
Sbjct: 137 VFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPVDGILQAVPHVIA 196

Query: 266 LFFIPTHFRTHIVLLFLE 284
           LF +P HF THI LLF+E
Sbjct: 197 LFIVPIHFTTHIGLLFME 214

[Close]

BLAST of Sgr021073 vs. TAIR 10
Match: AT3G02590.1 (Fatty acid hydroxylase superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 8.7e-46
Identity = 86/140 (61.43%), Postives = 103/140 (73.57%), Query Frame = 0


Query: 144 QHSIPSGKAVLLQIGVTAKAMPLYCTLPTFSEYVVEHDWTKCYPRVHDVGWLAYGFHIIA 203
           + SIP+ KA+LLQI V  KAMP Y  LP  SEY++EH WTKCY  +    W     +I  
Sbjct: 76  KESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWFLCFLYIAL 135

Query: 204 YLTFIEFFIYWMHRGLHDIKPLYKYLHAKHHMFNKKVTLSPFAGLAFHPVDGILQAIPHL 263
           YL  +EF IYW+H+ LHDIK LYK+LHA HHM+NK+ TLSPFAGLAFHP+DGILQAIPH+
Sbjct: 136 YLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDGILQAIPHV 195

Query: 264 FALFFIPTHFRTHIVLLFLE 284
            ALF +P H  TH+ LLFLE
Sbjct: 196 IALFIVPIHLITHLSLLFLE 215

The following BLAST results are available for this feature:

BLAST of Sgr021073 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148152.1	7.1e-66	86.33	delta(7)-sterol-C5(6)-desaturase-like isoform X2 [Momordica charantia]	[more]
XP_022148151.1	7.8e-65	86.23	delta(7)-sterol-C5(6)-desaturase-like isoform X1 [Momordica charantia]	[more]
XP_004150515.1	3.9e-64	86.96	delta(7)-sterol-C5(6)-desaturase [Cucumis sativus] >KGN54626.1 hypothetical prot...	[more]
XP_038894105.1	5.1e-64	86.23	delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894106.1 delta(...	[more]
XP_008444093.1	8.6e-64	86.96	PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo]	[more]

BLAST of Sgr021073 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZT29	8.7e-51	65.94	Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q39208	7.6e-47	63.77	Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=STE1 PE=1 ...	[more]
Q9M883	1.2e-44	61.43	Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=H...	[more]
Q9EQS5	7.0e-16	34.62	Lathosterol oxidase OS=Rattus norvegicus OX=10116 GN=Sc5d PE=1 SV=1	[more]
O88822	5.9e-15	33.33	Lathosterol oxidase OS=Mus musculus OX=10090 GN=Sc5d PE=2 SV=2	[more]

BLAST of Sgr021073 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D4A6	3.4e-66	86.33	delta(7)-sterol-C5(6)-desaturase-like isoform X2 OS=Momordica charantia OX=3673 ...	[more]
A0A6J1D4I4	3.8e-65	86.23	delta(7)-sterol-C5(6)-desaturase-like isoform X1 OS=Momordica charantia OX=3673 ...	[more]
A0A0A0L3B9	1.9e-64	86.96	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A1S3BAC7	4.2e-64	86.96	delta(7)-sterol-C5(6)-desaturase-like OS=Cucumis melo OX=3656 GN=LOC103487532 PE...	[more]
A0A6J1KDR9	9.3e-64	84.06	delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC11149293...	[more]

BLAST of Sgr021073 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02580.1	5.4e-48	63.77	sterol 1	[more]
AT3G02590.1	8.7e-46	61.43	Fatty acid hydroxylase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 202..283 e-value: 1.0E-10 score: 42.1
None	No IPR available	PANTHER	PTHR11863:SF160	DELTA(7)-STEROL-C5(6)-DESATURASE	coord: 144..284
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 144..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr021073.1	Sgr021073.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000248	C-5 sterol desaturase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity