Sgr021019 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr021019 vs. NCBI nr
Match: XP_008455663.1 (PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo] >XP_008455664.1 PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo] >XP_008455665.1 PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo] >XP_016901783.1 PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo])

HSP 1 Score: 1786.5 bits (4626), Expect = 0.0e+00
Identity = 895/973 (91.98%), Postives = 929/973 (95.48%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGPFPLLDTAIE  PRCP+IT  SSY RRSS+CH ++ TVSATR  KVSYI+NL  KPKT
Sbjct: 1   MGPFPLLDTAIEIFPRCPIITSRSSYGRRSSHCHLLVTTVSATRNWKVSYIENLQSKPKT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           VVFSS DNS+DH+TD V DADGF+TGRSEVLET EDEIL VKKALLESQTRQ+AVE+ERD
Sbjct: 61  VVFSSRDNSNDHLTDLVNDADGFTTGRSEVLETGEDEILAVKKALLESQTRQKAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELA+SELE ARSLFNK+++ESVGEKFALESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAISELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATS ILEDAQ+RVSVAETSA+E SY+IEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SF+EQSK AIEKALDVAEKA  HAKK +ATFTDEVYPLD ITSI+SENIKLKGVVNELES
Sbjct: 241 SFLEQSKIAIEKALDVAEKASVHAKKAMATFTDEVYPLDGITSIQSENIKLKGVVNELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HL LARTDVDNLKLELE ARAQATASEIRAKNAEK L+EFQELSREKI QQEGEIKLMME
Sbjct: 301 HLSLARTDVDNLKLELENARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKDVADKKKAASKAFK ELEGIKSAI+AAKETAHSKD+AYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDVADKKKAASKAFKVELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRADMAES LLKE T+GKD+EDA Y VNGGRIDLLTDDESQKWKLL+DGPRREIPQ
Sbjct: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLTDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKIDVTE S SKFRSLDLPKLEEVWSIAQEKPKVGD LIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEISASKFRSLDLPKLEEVWSIAQEKPKVGDALIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKT+QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALQRKTKQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYG+EEELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIE SNP FAIGEYWDSLAY
Sbjct: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH NL YNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWR+IDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRMIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTP IFYDHFYDFGIR++I
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREMI 900

Query: 901 TELIEARQRAGIHCRSSINIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
            ELIEARQRAGIHCRSS+ IYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLWLRQ
Sbjct: 961 VDKGSDYQLWLRQ 973

BLAST of Sgr021019 vs. NCBI nr
Match: XP_038893540.1 (uncharacterized protein LOC120082441 [Benincasa hispida])

HSP 1 Score: 1784.2 bits (4620), Expect = 0.0e+00
Identity = 899/973 (92.39%), Postives = 922/973 (94.76%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGPFPLLDTA+E  PRCP+I P SSY RRSSNC+HIL TVS TR  KVSYI NL  KPKT
Sbjct: 1   MGPFPLLDTALEIFPRCPIIIPASSYGRRSSNCYHILTTVSPTRNWKVSYIGNLHSKPKT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           V FSS DNSSD +TD V D DGFSTGRSEVLETEEDEIL VK ALLESQTRQEAVE+ERD
Sbjct: 61  VAFSSRDNSSDRLTDLVDDGDGFSTGRSEVLETEEDEILAVKMALLESQTRQEAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELAVSELE ARSLFNK+++ESVGEKF+LESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKKLEESVGEKFSLESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATS ILEDAQ+RVSVAETSAVE SY+IEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVETSYEIEKQIRDATEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SFVEQSK AIEKALDVAEKA  HAKK VATFTDEVYPLDEI SI+SENI+LKGVVNELES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHAKKAVATFTDEVYPLDEIASIQSENIQLKGVVNELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HL LARTDVD LKLELEQARAQATASEIRAKNAEKA +EFQELSREK IQQEGEIKLMME
Sbjct: 301 HLSLARTDVDTLKLELEQARAQATASEIRAKNAEKAWVEFQELSREKTIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKDVADKKKAASKAFKAELEGIKSAI AAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDVADKKKAASKAFKAELEGIKSAIHAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRADMAES LLKE TLG D+EDA Y VNGGRIDLLTDDESQKWKLLSDGPRREIPQ
Sbjct: 421 KMWQQRADMAESYLLKERTLGIDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRP FPPRKID+TE STSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPMFPPRKIDITEISTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKT QWQRTPD+TKLEPGTGTGHEIVFQGFNWESWRRRWYLELA 
Sbjct: 541 ETIEKKRKALERALQRKTIQWQRTPDKTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAT 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYGSEEELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIE SNP FAIGEYWDSLAY
Sbjct: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH +L YNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGDLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTP IFYDHFYDFGIR++I
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREMI 900

Query: 901 TELIEARQRAGIHCRSSINIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
            ELIE RQRAGIHCRSS+ IYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF
Sbjct: 901 NELIEVRQRAGIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLWLRQ
Sbjct: 961 VDKGSDYQLWLRQ 973

BLAST of Sgr021019 vs. NCBI nr
Match: XP_004137176.1 (uncharacterized protein LOC101217339 [Cucumis sativus] >KAE8649296.1 hypothetical protein Csa_014829 [Cucumis sativus])

HSP 1 Score: 1767.3 bits (4576), Expect = 0.0e+00
Identity = 889/973 (91.37%), Postives = 923/973 (94.86%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGPFPLLD AIE  PRCP+IT  SSY RRSS+CH  L  VS+TR  KVSYI+NL  KPKT
Sbjct: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           V FSS DNS+DH+TD V DADGFSTGRSEVLET EDEIL VKKALLESQTRQEAVE+ERD
Sbjct: 61  VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELAVSELE ARSLFNK+++ESVGEKFALESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATS ILEDAQ+RVSVAETSA+E SY+IEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SF+EQSK AIEKALDVAEKA  HAKK +ATFTDEVYPLDEI SI+SENIKLKGV+NELES
Sbjct: 241 SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HL LAR++V+NLKLELEQARAQATASEIRAKNAEK L+EFQELSREKI QQEGEIKLMME
Sbjct: 301 HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKDVADKKKAASK FKAELEGIKSAI+AAKETAHSKD+AYMRRCEALQRLLRASEA T
Sbjct: 361 KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRADMAES LLKE T+GKD+EDA Y VNGGRIDLLTDDESQKWKLLSDGPRREIPQ
Sbjct: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKIDVTE S SKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYG+ EELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSP+GVWNIFGGKL WGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPSGVWNIFGGKLTWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIE SNP FAIGEYWDSLAY
Sbjct: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH NL YNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWR+IDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRMIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTP IFYDHFYDFGIR++I
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREMI 900

Query: 901 TELIEARQRAGIHCRSSINIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
            ELIEARQRAGIHCRSS+ IYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDG+WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGSWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLWLRQ
Sbjct: 961 VDKGSDYQLWLRQ 973

BLAST of Sgr021019 vs. NCBI nr
Match: KAG7036792.1 (Alpha-amylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1736.9 bits (4497), Expect = 0.0e+00
Identity = 875/973 (89.93%), Postives = 908/973 (93.32%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGPFPLLD AIE LPRCP++TPGSSY RRSSNCHH LRTVS T KRKVSY DN L KP T
Sbjct: 1   MGPFPLLDAAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVSVTWKRKVSYTDNFLHKPIT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           V FSS DNS+D            STGRSE+L +EEDEIL VKKALLESQTRQEAVE+E D
Sbjct: 61  VAFSSRDNSNDP-----------STGRSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELE ARSLFNK+++ESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATS ILEDAQ R+S AETSAVEASY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRISAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SFVEQSK AIEKALDVAEKA  HA K VATFTDEVYPLDEI SI+SE++KLKGVV+ELES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYPLDEIASIQSESVKLKGVVDELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HL LAR DVDNLKLELEQARAQATASEIRAKNAEKALLEFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARAQATASEIRAKNAEKALLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRA+MAES L KE TLGKD+E+A Y VNGGRIDLLT+DESQKWKLLSDGPRREIPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYVVNGGRIDLLTNDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID++E STSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDISEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYGSEEELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIE SNPTFAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSNPTFAIGEYWDSLAY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH NL YNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGTP +FYDHFYDFGIR+II
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGTPTVFYDHFYDFGIREII 900

Query: 901 TELIEARQRAGIHCRSSINIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
            ELIEARQRAGIHCRSS+ I+HANNEGYVAQVGDTLVMKLGHFDWNPSKEN LDG WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIFHANNEGYVAQVGDTLVMKLGHFDWNPSKENQLDGKWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLW+RQ
Sbjct: 961 VDKGSDYQLWMRQ 962

BLAST of Sgr021019 vs. NCBI nr
Match: XP_022948370.1 (uncharacterized protein LOC111452072 isoform X1 [Cucurbita moschata] >XP_022948371.1 uncharacterized protein LOC111452072 isoform X1 [Cucurbita moschata] >XP_022948373.1 uncharacterized protein LOC111452072 isoform X1 [Cucurbita moschata] >XP_022948374.1 uncharacterized protein LOC111452072 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1734.2 bits (4490), Expect = 0.0e+00
Identity = 874/973 (89.83%), Postives = 908/973 (93.32%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGPFPLLD AIE LPRCP++TPGSSY RRSSNCHH LRTV+ T KRKVSY DN L KP T
Sbjct: 1   MGPFPLLDAAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVTVTWKRKVSYTDNFLHKPIT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           V FSS D+S+D            STGRSE+L +EEDEIL VKKALLESQTRQEAVE+E D
Sbjct: 61  VAFSSRDHSNDP-----------STGRSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELE ARSLFNK+++ESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATS ILEDAQ RVS AETSAVEASY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRVSAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SFVEQSK AIEKALDVAEKA  HA K VATFTDEVYPLDEI SI+SE++KLKGVV+ELES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYPLDEIASIQSESVKLKGVVDELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HL LAR DVDNLKLELEQARAQATASEIRAKNAEKALLEFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARAQATASEIRAKNAEKALLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRA+MAES L KE TLGKD+E+A Y VNGGRIDLLT+DESQKWKLLSDGPRREIPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYVVNGGRIDLLTNDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID++E STSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDISEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYGSEEELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIE SNPTFAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSNPTFAIGEYWDSLAY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH NL YNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGTP +FYDHFYDFGIR+II
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGTPTVFYDHFYDFGIREII 900

Query: 901 TELIEARQRAGIHCRSSINIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
            ELIEARQRAGIHCRSS+ I+HANNEGYVAQVGDTLVMKLGHFDWNPSKEN LDG WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIFHANNEGYVAQVGDTLVMKLGHFDWNPSKENQLDGKWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLW+RQ
Sbjct: 961 VDKGSDYQLWMRQ 962

BLAST of Sgr021019 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.3e-153
Identity = 247/404 (61.14%), Postives = 302/404 (74.75%), Query Frame = 0

Query: 569 KLEPGTGTGHEIVFQGFNWESWRR-RWYLELAAKASDLSQSGITAVWLPPPTESVAPQGY 628
           K+  GTG+G EI+ QGFNWES +  RWYLEL  KA +L+  G T +WLPPPTESV+P+GY
Sbjct: 486 KISSGTGSGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGY 545

Query: 629 MPSDLHNLNSSYGSEEELKYCIEEFHSQDILALGDVVLNHRCAHKQSPNGVWNIFGGKLP 688
           MP DL+NLNS YG+ +ELK  +++FH   I  LGD VLNHRCAH ++ NGVWN+FGG+L 
Sbjct: 546 MPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLN 605

Query: 689 WGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDHSQDFVRSDIKEWLNWLRNDIGFDGWRL 748
           W   A+V DDP+FQGRGN SSGD FHAAPNIDHSQDFVR DIKEWL W+  ++G+DGWRL
Sbjct: 606 WDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRL 665

Query: 749 DFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAYEHENLGYNQDAHRQRIVNWINATGGTS 808
           DFVRGF G YVK+Y++AS P FA+GEYWDSL+Y +  + YNQDAHRQRIV+WINAT G +
Sbjct: 666 DFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAA 725

Query: 809 SAFDVTTKGILHSALHN-QYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGSTQGHWPFP 868
            AFDVTTKGILH+AL   +YWRL DP+GKP GVVGWWPSRAVTF+ENHDTGSTQGHW FP
Sbjct: 726 GAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 785

Query: 869 RDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDIITELIEARQRAGIHCRSSINIYHANNE 928
             K  QGYAYILTHPGTP +F+DH +       I  L+  R R  +HCRS +NI  +  +
Sbjct: 786 EGKEMQGYAYILTHPGTPAVFFDHIFS-DYHSEIAALLSLRNRQKLHCRSEVNIDKSERD 845

Query: 929 GYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKFVDKGSDYQLW 971
            Y A + + + MK+G   + P   +    NW   V+ G DY++W
Sbjct: 846 VYAAIIDEKVAMKIGPGHYEPPNGSQ---NWSVAVE-GRDYKVW 884

BLAST of Sgr021019 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 3.6e-124
Identity = 207/409 (50.61%), Postives = 268/409 (65.53%), Query Frame = 0

Query: 566 DQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQ 625
           DQT +      G E++ Q +NWES +  W+  L  K  D+++SG T+ WLPPP++S+AP+
Sbjct: 13  DQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPE 72

Query: 626 GYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILALGDVVLNHRCAHKQSPNGVWNIFGG- 685
           GY+P DL++LNS+YGSE  LK  + +     + A+ D+V+NHR    +   G++N + G 
Sbjct: 73  GYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGI 132

Query: 686 KLPWGPEAIV-CDDPNFQGRGNPSSGDIFHAAPNIDHSQDFVRSDIKEWLNWLRNDIGFD 745
            LPW   A+  C      G GN S+GD F+  PN+DH+Q FVR DI  WL WLRN +GF 
Sbjct: 133 SLPWDEHAVTSCTG----GLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQ 192

Query: 746 GWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAYEHENLGYNQDAHRQRIVNWINAT 805
            +R DF RG+S  YVKEYI A+ P F++GE WDS  Y    L YNQD+HRQRI++WI+AT
Sbjct: 193 DFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDAT 252

Query: 806 GGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGSTQGHW 865
           G  S+AFD TTKGIL  A+  QYWRL D QGKP GV+GWWPSRAVTFL+NHDTGSTQ HW
Sbjct: 253 GQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHW 312

Query: 866 PFPRDKLAQGYAYILTHPGTPVIFYDHFYDFG--IRDIITELIEARQRAGIHCRSSINIY 925
           PFP   + +GYAYILTHPG P +FYDHFYD+G  I D I +LI+ R+R  IH RS++ + 
Sbjct: 313 PFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVL 372

Query: 926 HANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKFVDKGSDYQLW 971
            A +  Y A VG+ + MKLG   W PS      G        G  Y +W
Sbjct: 373 KAESNLYAAIVGEKICMKLGDGSWCPS------GRDWTLATSGHRYAVW 411

BLAST of Sgr021019 vs. ExPASy Swiss-Prot
Match: P00693 (Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.6e-114
Identity = 186/382 (48.69%), Postives = 262/382 (68.59%), Query Frame = 0

Query: 573 GTGTGHEIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPS 632
           G  +GH+++FQGFNWESW++   WY  +  K  D++ +G+T VWLPPP+ SV+ +GYMP 
Sbjct: 20  GLASGHQVLFQGFNWESWKQSGGWYNMMMGKVDDIAAAGVTHVWLPPPSHSVSNEGYMPG 79

Query: 633 DLHNLNSS-YGSEEELKYCIEEFHSQDILALGDVVLNHRCAHKQSPNGVWNIF-----GG 692
            L+++++S YG+  ELK  I   H + + A+ D+V+NHRCA  +   G++ IF      G
Sbjct: 80  RLYDIDASKYGNAAELKSLIGALHGKGVQAIADIVINHRCADYKDSRGIYCIFEGGTSDG 139

Query: 693 KLPWGPEAIVCDDPNF-QGRGNPSSGDIFHAAPNIDHSQDFVRSDIKEWLNWLRNDIGFD 752
           +L WGP  I  DD  +  G  N  +G  F AAP+IDH  D V+ ++KEWL WL++D+GFD
Sbjct: 140 RLDWGPHMICRDDTKYSDGTANLDTGADFAAAPDIDHLNDRVQRELKEWLLWLKSDLGFD 199

Query: 753 GWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAYEHENL-GYNQDAHRQRIVNWINA 812
            WRLDF RG+S    K YI+ ++P+ A+ E WD++A   +    Y+QDAHRQ +VNW++ 
Sbjct: 200 AWRLDFARGYSPEMAKVYIDGTSPSLAVAEVWDNMATGGDGKPNYDQDAHRQNLVNWVDK 259

Query: 813 TGGTSSA---FDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGST 872
            GG +SA   FD TTKGIL++A+  + WRLIDPQGK  GV+GWWP++A TF++NHDTGST
Sbjct: 260 VGGAASAGMVFDFTTKGILNAAVEGELWRLIDPQGKAPGVMGWWPAKAATFVDNHDTGST 319

Query: 873 QGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDIITELIEARQRAGIHCRSSIN 932
           Q  WPFP DK+ QGYAYILTHPG P IFYDHF+++G +D I  L+  R+R GI   S++ 
Sbjct: 320 QAMWPFPSDKVMQGYAYILTHPGIPCIFYDHFFNWGFKDQIAALVAIRKRNGITATSALK 379

Query: 933 IYHANNEGYVAQVGDTLVMKLG 942
           I     + YVA++   +V+K+G
Sbjct: 380 ILMHEGDAYVAEIDGKVVVKIG 401

BLAST of Sgr021019 vs. ExPASy Swiss-Prot
Match: A2YGY2 (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica OX=39946 GN=AMYC2 PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 7.6e-114
Identity = 196/384 (51.04%), Postives = 261/384 (67.97%), Query Frame = 0

Query: 573 GTGTGHEIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPS 632
           G  +G +I+FQGFNWESWR+   WY  L  K  D+  +G+T VWLPPP+ SV+ QGYMP 
Sbjct: 17  GLASGDKILFQGFNWESWRQSGGWYNLLMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPG 76

Query: 633 DLHNLNSS-YGSEEELKYCIEEFHSQDILALGDVVLNHRCAHKQSPNGVWNIF-----GG 692
            L++L++S YG+  ELK  I   H + I A+ DVV+NHRCA  +   G++ IF      G
Sbjct: 77  RLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDG 136

Query: 693 KLPWGPEAIVCDDPNF-QGRGNPSSGDIFHAAPNIDHSQDFVRSDIKEWLNWLRND-IGF 752
           +L WGP  I  DD  F  G GN  +G  F AAP+IDH    V+ ++ +WL WL++D +GF
Sbjct: 137 RLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLNGVVQRELTDWLLWLKSDEVGF 196

Query: 753 DGWRLDFVRGFSGTYVKEYIEASNPT-FAIGEYWDSLAYEHENL-GYNQDAHRQRIVNWI 812
           D WRLDF RG+S    K YIE + P   A+ E WDS+AY  +    YNQDAHRQ +V+W+
Sbjct: 197 DAWRLDFARGYSPEVAKVYIEGTTPVGLAVAELWDSMAYGGDGKPEYNQDAHRQALVDWV 256

Query: 813 NATGGTSSA---FDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTG 872
           +  GGT+SA   FD TTKGI+++A+  + WRLID QGK  GV+GWWP++AVTF++NHDTG
Sbjct: 257 DRVGGTASAGMVFDFTTKGIMNTAVEGELWRLIDQQGKAPGVIGWWPAKAVTFVDNHDTG 316

Query: 873 STQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDIITELIEARQRAGIHCRSS 932
           STQ  WPFP DK+ QGYAYILTHPG P IFYDHF+D+G+++ I  L+  RQR G+   SS
Sbjct: 317 STQQMWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDWGLKEQIAALVAVRQRNGVTATSS 376

Query: 933 INIYHANNEGYVAQVGDTLVMKLG 942
           + I   + + YVA++   +VMK+G
Sbjct: 377 LKIMLHDADAYVAEIDGKVVMKIG 400

BLAST of Sgr021019 vs. ExPASy Swiss-Prot
Match: Q0D9J1 (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY2A PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 7.6e-114
Identity = 196/384 (51.04%), Postives = 261/384 (67.97%), Query Frame = 0

Query: 573 GTGTGHEIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPS 632
           G  +G +I+FQGFNWESWR+   WY  L  K  D+  +G+T VWLPPP+ SV+ QGYMP 
Sbjct: 17  GLASGDKILFQGFNWESWRQSGGWYNLLMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPG 76

Query: 633 DLHNLNSS-YGSEEELKYCIEEFHSQDILALGDVVLNHRCAHKQSPNGVWNIF-----GG 692
            L++L++S YG+  ELK  I   H + I A+ DVV+NHRCA  +   G++ IF      G
Sbjct: 77  RLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDG 136

Query: 693 KLPWGPEAIVCDDPNF-QGRGNPSSGDIFHAAPNIDHSQDFVRSDIKEWLNWLRND-IGF 752
           +L WGP  I  DD  F  G GN  +G  F AAP+IDH    V+ ++ +WL WL++D +GF
Sbjct: 137 RLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLNGVVQRELTDWLLWLKSDEVGF 196

Query: 753 DGWRLDFVRGFSGTYVKEYIEASNPT-FAIGEYWDSLAYEHENL-GYNQDAHRQRIVNWI 812
           D WRLDF RG+S    K YIE + P   A+ E WDS+AY  +    YNQDAHRQ +V+W+
Sbjct: 197 DAWRLDFARGYSPEVAKVYIEGTTPVGLAVAELWDSMAYGGDGKPEYNQDAHRQALVDWV 256

Query: 813 NATGGTSSA---FDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTG 872
           +  GGT+SA   FD TTKGI+++A+  + WRLID QGK  GV+GWWP++AVTF++NHDTG
Sbjct: 257 DRVGGTASAGMVFDFTTKGIMNTAVEGELWRLIDQQGKAPGVIGWWPAKAVTFVDNHDTG 316

Query: 873 STQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDIITELIEARQRAGIHCRSS 932
           STQ  WPFP DK+ QGYAYILTHPG P IFYDHF+D+G+++ I  L+  RQR G+   SS
Sbjct: 317 STQQMWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDWGLKEQIAALVAVRQRNGVTATSS 376

Query: 933 INIYHANNEGYVAQVGDTLVMKLG 942
           + I   + + YVA++   +VMK+G
Sbjct: 377 LKIMLHDADAYVAEIDGKVVMKIG 400

BLAST of Sgr021019 vs. ExPASy TrEMBL
Match: A0A1S3C1E0 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103495777 PE=4 SV=1)

HSP 1 Score: 1786.5 bits (4626), Expect = 0.0e+00
Identity = 895/973 (91.98%), Postives = 929/973 (95.48%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGPFPLLDTAIE  PRCP+IT  SSY RRSS+CH ++ TVSATR  KVSYI+NL  KPKT
Sbjct: 1   MGPFPLLDTAIEIFPRCPIITSRSSYGRRSSHCHLLVTTVSATRNWKVSYIENLQSKPKT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           VVFSS DNS+DH+TD V DADGF+TGRSEVLET EDEIL VKKALLESQTRQ+AVE+ERD
Sbjct: 61  VVFSSRDNSNDHLTDLVNDADGFTTGRSEVLETGEDEILAVKKALLESQTRQKAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELA+SELE ARSLFNK+++ESVGEKFALESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAISELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATS ILEDAQ+RVSVAETSA+E SY+IEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SF+EQSK AIEKALDVAEKA  HAKK +ATFTDEVYPLD ITSI+SENIKLKGVVNELES
Sbjct: 241 SFLEQSKIAIEKALDVAEKASVHAKKAMATFTDEVYPLDGITSIQSENIKLKGVVNELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HL LARTDVDNLKLELE ARAQATASEIRAKNAEK L+EFQELSREKI QQEGEIKLMME
Sbjct: 301 HLSLARTDVDNLKLELENARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKDVADKKKAASKAFK ELEGIKSAI+AAKETAHSKD+AYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDVADKKKAASKAFKVELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRADMAES LLKE T+GKD+EDA Y VNGGRIDLLTDDESQKWKLL+DGPRREIPQ
Sbjct: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLTDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKIDVTE S SKFRSLDLPKLEEVWSIAQEKPKVGD LIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEISASKFRSLDLPKLEEVWSIAQEKPKVGDALIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKT+QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALQRKTKQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYG+EEELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIE SNP FAIGEYWDSLAY
Sbjct: 721 SQDFVRRDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH NL YNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWR+IDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRMIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTP IFYDHFYDFGIR++I
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPTIFYDHFYDFGIREMI 900

Query: 901 TELIEARQRAGIHCRSSINIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
            ELIEARQRAGIHCRSS+ IYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLWLRQ
Sbjct: 961 VDKGSDYQLWLRQ 973

BLAST of Sgr021019 vs. ExPASy TrEMBL
Match: A0A6J1G955 (1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111452072 PE=4 SV=1)

HSP 1 Score: 1734.2 bits (4490), Expect = 0.0e+00
Identity = 874/973 (89.83%), Postives = 908/973 (93.32%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGPFPLLD AIE LPRCP++TPGSSY RRSSNCHH LRTV+ T KRKVSY DN L KP T
Sbjct: 1   MGPFPLLDAAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVTVTWKRKVSYTDNFLHKPIT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           V FSS D+S+D            STGRSE+L +EEDEIL VKKALLESQTRQEAVE+E D
Sbjct: 61  VAFSSRDHSNDP-----------STGRSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELE ARSLFNK+++ESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATS ILEDAQ RVS AETSAVEASY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRVSAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SFVEQSK AIEKALDVAEKA  HA K VATFTDEVYPLDEI SI+SE++KLKGVV+ELES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYPLDEIASIQSESVKLKGVVDELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HL LAR DVDNLKLELEQARAQATASEIRAKNAEKALLEFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARAQATASEIRAKNAEKALLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRA+MAES L KE TLGKD+E+A Y VNGGRIDLLT+DESQKWKLLSDGPRREIPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYVVNGGRIDLLTNDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID++E STSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDISEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYGSEEELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIE SNPTFAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSNPTFAIGEYWDSLAY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH NL YNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGTP +FYDHFYDFGIR+II
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGTPTVFYDHFYDFGIREII 900

Query: 901 TELIEARQRAGIHCRSSINIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
            ELIEARQRAGIHCRSS+ I+HANNEGYVAQVGDTLVMKLGHFDWNPSKEN LDG WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIFHANNEGYVAQVGDTLVMKLGHFDWNPSKENQLDGKWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLW+RQ
Sbjct: 961 VDKGSDYQLWMRQ 962

BLAST of Sgr021019 vs. ExPASy TrEMBL
Match: A0A6J1KAV6 (1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111492635 PE=4 SV=1)

HSP 1 Score: 1721.4 bits (4457), Expect = 0.0e+00
Identity = 869/973 (89.31%), Postives = 902/973 (92.70%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGPFPLLD AIE LPRCP++TPGSSY RRSSNCHH LRTVS T KRKVSY DN L KP T
Sbjct: 1   MGPFPLLDVAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVSVTWKRKVSYTDNFLHKPIT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           V FSS DNS+D            STG SE+L +EEDEIL VKKALLESQTRQEAVE+E D
Sbjct: 61  VAFSSRDNSNDP-----------STGGSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELE ARSLFNK+++ESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATS ILEDAQ RVS AETSAVEASY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRVSAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SFVEQSK AIEKALDVAEKA  HA K VATFTDEVY LDEI SI+SE++KLKGVV+ELES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYALDEIASIQSESVKLKGVVDELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HL LAR DVDNLKLELEQARA+ATASEIRAKNAE  LLEFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARARATASEIRAKNAETVLLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRA+MAES L KE TLGKD+E+A Y VNGGRIDLLT+DESQKWKLLSDGPRR IPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYIVNGGRIDLLTNDESQKWKLLSDGPRRAIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID+TE STSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDITEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYGSEEELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIE S+PTFAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSDPTFAIGEYWDSLAY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH NL YNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDII 900
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGTP +FYDHFYDFGIR+II
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGTPTVFYDHFYDFGIREII 900

Query: 901 TELIEARQRAGIHCRSSINIYHANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKF 960
            ELIEARQRAGIHCRSS+ I+HANNEGYVAQVGD LVMKLGHFDWNPSKEN LDG WQKF
Sbjct: 901 NELIEARQRAGIHCRSSVKIFHANNEGYVAQVGDNLVMKLGHFDWNPSKENQLDGKWQKF 960

Query: 961 VDKGSDYQLWLRQ 974
           VDKGSDYQLW+RQ
Sbjct: 961 VDKGSDYQLWMRQ 962

BLAST of Sgr021019 vs. ExPASy TrEMBL
Match: A0A6J1DP26 (uncharacterized protein LOC111022188 OS=Momordica charantia OX=3673 GN=LOC111022188 PE=4 SV=1)

HSP 1 Score: 1625.9 bits (4209), Expect = 0.0e+00
Identity = 820/883 (92.87%), Postives = 848/883 (96.04%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGP PLLDTAIEFLPRCP+I+ GSSYCRRSSN H ILRTVSA RK K+SY+D LLCKPKT
Sbjct: 1   MGPLPLLDTAIEFLPRCPIISAGSSYCRRSSNYHQILRTVSAARKWKLSYVDKLLCKPKT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           VVFSSPDNSSD++TDWV DADGFSTGRSEVL+TEEDEIL VKK LLESQTRQEAVE+ERD
Sbjct: 61  VVFSSPDNSSDNLTDWVDDADGFSTGRSEVLQTEEDEILAVKKVLLESQTRQEAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QL+ERLARYEAKQKEYVATILHDKELAVSELE ARSLFNK++ +SVGEKFALESKLVLAK
Sbjct: 121 QLIERLARYEAKQKEYVATILHDKELAVSELEAARSLFNKRLHDSVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATS ILEDAQ+RVSVAETSAVEASYQIEKQIRDA EGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAVEASYQIEKQIRDAIEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SFVEQSK+ IEKALDVAEKAG HAKKVV TFTDEVYPLDEI S++SENIKLKGV+NELES
Sbjct: 241 SFVEQSKQTIEKALDVAEKAGAHAKKVVGTFTDEVYPLDEIASVQSENIKLKGVINELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HLLL R DVDNLKLELEQARAQATASEIRAKN+EK LLEFQELSREKII+QEGEIK MME
Sbjct: 301 HLLLTRADVDNLKLELEQARAQATASEIRAKNSEKILLEFQELSREKIIKQEGEIKSMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           K KKD+ADKKKAA+KAFKAELEGIKSAIEAAKETA+SKDNAY+RRCEALQRLLRASEAAT
Sbjct: 361 KFKKDLADKKKAATKAFKAELEGIKSAIEAAKETAYSKDNAYVRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           K WQQRADMAESLLLKE T GK DEDA Y VNGGRIDLLTDDESQKWKLLSDGPRREIPQ
Sbjct: 421 KTWQQRADMAESLLLKERTPGKVDEDAVYVVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKIDVTE STSKFRSLDLPK+EEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEVSTSKFRSLDLPKIEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERAL+RKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALERKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYGSEEELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSL Y
Sbjct: 721 SQDFVRKDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLGY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH NL YNQDAHRQRI+NWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIINWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGT 884
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGT
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGT 883

BLAST of Sgr021019 vs. ExPASy TrEMBL
Match: A0A6J1G9N4 (uncharacterized protein LOC111452072 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452072 PE=4 SV=1)

HSP 1 Score: 1555.8 bits (4027), Expect = 0.0e+00
Identity = 794/883 (89.92%), Postives = 823/883 (93.20%), Query Frame = 0

Query: 1   MGPFPLLDTAIEFLPRCPVITPGSSYCRRSSNCHHILRTVSATRKRKVSYIDNLLCKPKT 60
           MGPFPLLD AIE LPRCP++TPGSSY RRSSNCHH LRTV+ T KRKVSY DN L KP T
Sbjct: 1   MGPFPLLDAAIEILPRCPIVTPGSSYGRRSSNCHHNLRTVTVTWKRKVSYTDNFLHKPIT 60

Query: 61  VVFSSPDNSSDHVTDWVADADGFSTGRSEVLETEEDEILGVKKALLESQTRQEAVEQERD 120
           V FSS D+S+D            STGRSE+L +EEDEIL VKKALLESQTRQEAVE+E D
Sbjct: 61  VAFSSRDHSNDP-----------STGRSEILGSEEDEILAVKKALLESQTRQEAVEKETD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELETARSLFNKQIQESVGEKFALESKLVLAK 180
           QLLE+L RYEAKQKEY+ATILHDKELAVSELE ARSLFNK+++ESVGEKFALESKLVLAK
Sbjct: 121 QLLEKLTRYEAKQKEYLATILHDKELAVSELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSQILEDAQHRVSVAETSAVEASYQIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAF+QATS ILEDAQ RVS AETSAVEASY+IEKQI DATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFEQATSHILEDAQLRVSAAETSAVEASYEIEKQISDATEGSML 240

Query: 241 SFVEQSKKAIEKALDVAEKAGTHAKKVVATFTDEVYPLDEITSIESENIKLKGVVNELES 300
           SFVEQSK AIEKALDVAEKA  HA K VATFTDEVYPLDEI SI+SE++KLKGVV+ELES
Sbjct: 241 SFVEQSKIAIEKALDVAEKASAHANKAVATFTDEVYPLDEIASIQSESVKLKGVVDELES 300

Query: 301 HLLLARTDVDNLKLELEQARAQATASEIRAKNAEKALLEFQELSREKIIQQEGEIKLMME 360
           HL LAR DVDNLKLELEQARAQATASEIRAKNAEKALLEFQ  S EKIIQQEGEIKLMME
Sbjct: 301 HLSLARADVDNLKLELEQARAQATASEIRAKNAEKALLEFQNSSMEKIIQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420
           KIKKD  DKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT
Sbjct: 361 KIKKDFTDKKKAASKAFKAELEGIKSAIEAAKETAHSKDNAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESLLLKETTLGKDDEDATYDVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRA+MAES L KE TLGKD+E+A Y VNGGRIDLLT+DESQKWKLLSDGPRREIPQ
Sbjct: 421 KMWQQRAEMAESFLSKERTLGKDNEEAAYVVNGGRIDLLTNDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEASTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKID++E STSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDISEVSTSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTRQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           E+IEKKRKALERALQRKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ESIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILAL 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDL+NLNSSYGSEEELKYCIEEFHSQD+LAL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGSEEELKYCIEEFHSQDLLAL 660

Query: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLPWGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDH 720
           GDVVLNHRCAHKQSPNGVWNIFGGKL WGPEAIVCDDPNFQGRGNP SGDIFHAAPNIDH
Sbjct: 661 GDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRGNPKSGDIFHAAPNIDH 720

Query: 721 SQDFVRSDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAY 780
           SQDFVR DIKEWLNWLR+DIGFDGWRLDFVRGFSGTYVKEYIE SNPTFAIGEYWDSLAY
Sbjct: 721 SQDFVRKDIKEWLNWLRSDIGFDGWRLDFVRGFSGTYVKEYIETSNPTFAIGEYWDSLAY 780

Query: 781 EHENLGYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840
           EH NL YNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV
Sbjct: 781 EHGNLCYNQDAHRQRIVNWINATGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVV 840

Query: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGT 884
           GWWPSRAVTFLENHDTGSTQGHWPFPRDKL QGYAYILTHPGT
Sbjct: 841 GWWPSRAVTFLENHDTGSTQGHWPFPRDKLVQGYAYILTHPGT 872

BLAST of Sgr021019 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 545.4 bits (1404), Expect = 9.1e-155
Identity = 247/404 (61.14%), Postives = 302/404 (74.75%), Query Frame = 0

Query: 569 KLEPGTGTGHEIVFQGFNWESWRR-RWYLELAAKASDLSQSGITAVWLPPPTESVAPQGY 628
           K+  GTG+G EI+ QGFNWES +  RWYLEL  KA +L+  G T +WLPPPTESV+P+GY
Sbjct: 486 KISSGTGSGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGY 545

Query: 629 MPSDLHNLNSSYGSEEELKYCIEEFHSQDILALGDVVLNHRCAHKQSPNGVWNIFGGKLP 688
           MP DL+NLNS YG+ +ELK  +++FH   I  LGD VLNHRCAH ++ NGVWN+FGG+L 
Sbjct: 546 MPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLN 605

Query: 689 WGPEAIVCDDPNFQGRGNPSSGDIFHAAPNIDHSQDFVRSDIKEWLNWLRNDIGFDGWRL 748
           W   A+V DDP+FQGRGN SSGD FHAAPNIDHSQDFVR DIKEWL W+  ++G+DGWRL
Sbjct: 606 WDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRL 665

Query: 749 DFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAYEHENLGYNQDAHRQRIVNWINATGGTS 808
           DFVRGF G YVK+Y++AS P FA+GEYWDSL+Y +  + YNQDAHRQRIV+WINAT G +
Sbjct: 666 DFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAA 725

Query: 809 SAFDVTTKGILHSALHN-QYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGSTQGHWPFP 868
            AFDVTTKGILH+AL   +YWRL DP+GKP GVVGWWPSRAVTF+ENHDTGSTQGHW FP
Sbjct: 726 GAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 785

Query: 869 RDKLAQGYAYILTHPGTPVIFYDHFYDFGIRDIITELIEARQRAGIHCRSSINIYHANNE 928
             K  QGYAYILTHPGTP +F+DH +       I  L+  R R  +HCRS +NI  +  +
Sbjct: 786 EGKEMQGYAYILTHPGTPAVFFDHIFS-DYHSEIAALLSLRNRQKLHCRSEVNIDKSERD 845

Query: 929 GYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKFVDKGSDYQLW 971
            Y A + + + MK+G   + P   +    NW   V+ G DY++W
Sbjct: 846 VYAAIIDEKVAMKIGPGHYEPPNGSQ---NWSVAVE-GRDYKVW 884

BLAST of Sgr021019 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 447.6 bits (1150), Expect = 2.6e-125
Identity = 207/409 (50.61%), Postives = 268/409 (65.53%), Query Frame = 0

Query: 566 DQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQ 625
           DQT +      G E++ Q +NWES +  W+  L  K  D+++SG T+ WLPPP++S+AP+
Sbjct: 13  DQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPE 72

Query: 626 GYMPSDLHNLNSSYGSEEELKYCIEEFHSQDILALGDVVLNHRCAHKQSPNGVWNIFGG- 685
           GY+P DL++LNS+YGSE  LK  + +     + A+ D+V+NHR    +   G++N + G 
Sbjct: 73  GYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGI 132

Query: 686 KLPWGPEAIV-CDDPNFQGRGNPSSGDIFHAAPNIDHSQDFVRSDIKEWLNWLRNDIGFD 745
            LPW   A+  C      G GN S+GD F+  PN+DH+Q FVR DI  WL WLRN +GF 
Sbjct: 133 SLPWDEHAVTSCTG----GLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQ 192

Query: 746 GWRLDFVRGFSGTYVKEYIEASNPTFAIGEYWDSLAYEHENLGYNQDAHRQRIVNWINAT 805
            +R DF RG+S  YVKEYI A+ P F++GE WDS  Y    L YNQD+HRQRI++WI+AT
Sbjct: 193 DFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDAT 252

Query: 806 GGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGSTQGHW 865
           G  S+AFD TTKGIL  A+  QYWRL D QGKP GV+GWWPSRAVTFL+NHDTGSTQ HW
Sbjct: 253 GQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHW 312

Query: 866 PFPRDKLAQGYAYILTHPGTPVIFYDHFYDFG--IRDIITELIEARQRAGIHCRSSINIY 925
           PFP   + +GYAYILTHPG P +FYDHFYD+G  I D I +LI+ R+R  IH RS++ + 
Sbjct: 313 PFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVL 372

Query: 926 HANNEGYVAQVGDTLVMKLGHFDWNPSKENHLDGNWQKFVDKGSDYQLW 971
            A +  Y A VG+ + MKLG   W PS      G        G  Y +W
Sbjct: 373 KAESNLYAAIVGEKICMKLGDGSWCPS------GRDWTLATSGHRYAVW 411

BLAST of Sgr021019 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 362.5 bits (929), Expect = 1.1e-99
Identity = 169/372 (45.43%), Postives = 243/372 (65.32%), Query Frame = 0

Query: 580 IVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLHNLNS 639
           ++FQ FNWESW++   +Y  L     D++ +GIT +WLPPP++SVAP+GY+P  L++LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 640 S-YGSEEELKYCIEEFHSQDILALGDVVLNHRCAHKQSPNGVWNIFGG-----KLPWGPE 699
           S YGSE ELK  I+  + + I AL D+V+NHR A ++     +  F G     +L W P 
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 700 AIVCDDPNFQGRGNPSSGDIFHAAPNIDHSQDFVRSDIKEWLNWLRNDIGFDGWRLDFVR 759
            +  +DP F G GN  +G  F  AP+IDH    V+ ++ EW+NWL+ +IGF GWR D+VR
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206

Query: 760 GFSGTYVKEYIEASNPTFAIGEYWDSLAYEHE-NLGYNQDAHRQRIVNWI-NATGGTSSA 819
           G++ +  K Y++ ++P FA+GE WD + Y  +  L Y+Q+ HR  +  WI  A GG  +A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266

Query: 820 FDVTTKGILHSALHNQYWRLIDPQGKPTGVVGWWPSRAVTFLENHDTGSTQGHWPFPRDK 879
           FD TTKGIL SA+  + WRL D QGKP G++G  P  AVTF++NHDT  T   W FP DK
Sbjct: 267 FDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDK 326

Query: 880 LAQGYAYILTHPGTPVIFYDHFYDFGIRDIITELIEARQRAGIHCRSSINIYHANNEGYV 939
           +  GY YILTHPGTP IFY+H+ ++G+++ I++L+  R + GI   SS+ I  A  + Y+
Sbjct: 327 VLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLYL 386

Query: 940 AQVGDTLVMKLG 942
           A + D ++MK+G
Sbjct: 387 AMIDDKVIMKIG 395