Sgr021002 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021002
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionmuscle M-line assembly protein unc-89-like isoform X2
Locationtig00153633: 283738 .. 285546 (+)
RNA-Seq ExpressionSgr021002
SyntenySgr021002
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGATGGTGGTAATTTGAGAAGATATTCTATGGGAAAAGAAAGCTCATTGAGTATTGATGAACAAAGTATTTCTCGTCAACGCAGATCCTCTACCGGTTCTTGCCATGATGTGTGCAAGTATGGACATAAACATTCATTCGAAACGAAGGCCAGAGATCCCCTACCGAAAAGAGCAATGAAAAAGCCACTTGATGGCCAAAATTCAGACCAGGTTGTAGCTGTGCTCAAGAGAGAGAAGCCCACTGTTCGCGTGACCAAGTTGAAGGCTTCACCTGATTCAGGGACATGTAGTACTGGTGGCACCAACATGGTGAAACGGGTAGTGCCTATAAATTCTCCTATCACGCAAAGTCCAGTAGAGATTGAAGTTATGAATGAAAGTAAGGAACTCATAGTGCCCGTGAATTCTCCTAGCAGGCGGAGTGCAGTAGAGATTGAAGTTATGAATGGAAGTAAGAAATTCGTAGTGCCCGTCAATTCTCCTAACGGGCGAAGTCCAGTAGAGATTGAAGTTATGAATGAAACTAAGGAACTGGTGGTGCCTGTAAATTCTCCTACCAAGCGAAGTCCAGTTGGGATTGAAGTTATGAATGATAAGGAACTGGTAGTGCCTGTAGATTCTCCTACCAGGCGAAGTCCAGTAGAGAATGAAGTTATGAATGAAAGTAAGGAACTGGTGGACAAGACAAAAACCCATAAACCCAAAATTCATAAAACTACAAAGCAAGTAGTTATTCTGTCTACAAAATCTGAAAGTTCGCCAAAACAAGCCTTGACCAATACTGGAAAAGCCAAATTTCCCAAAAGGCTCGACAGTTTGTTGAAGCCGAATTCTTCAAAATTAAAATCAGTGACCTCTTCTGGTTTTTCTGGGAATATTGCAGTCCACAGAAACAACAGTTCTAAACCAGGTGAAGAAGGGAGGACCTCGAAAGGAATTGGAACGAAAGTAGCAGGAAATTCAGTAGTCATGTCAGGTGCAAAACCTGTTGATGCTGTTGCAAACTTACCTGCAATAAAGAACGAGAACTCGAAAGTTGTCTCTCGTGTTGTAAGTCAGAACAAAACAAGAAGAGCTCAAACCAAGGATGCCCCGAATGTGGAATTACGGAAGAAAACCTTGCATGTCAGCGATTCTGAAACTAAGAAGGCCAAGGTTGTAGAATCAGACCAAAATATAAAGCCTGCTTTGAAGCTGTGTCCAAAGCCACCATCATCACCATACAAGTCATCATCCTTAGCAGATTGCCCATCTCTTTCGCCTCGTAAAGAAAACTGTGGCGAAACTAAATATAAAAGAAGTGAAGCAAATGCCACTTTTTCGAGACCCAACAAACAAGGTGGCATAAAAAAAGAAGAGGCTCACAATGGGAATAAAAAAGGCAGGTCCCCGAGAATGCTTCCAACCAAAGGAAAGGATTCTTCATCTTTGAACTTAAATTTCAGGAACGGAAAGGTAGTCAACCTTCACTCCGAAAGTCCTAGTGCAAGGAGGCTCAAATTTATGCGAGGAAGATCGCTCGGGGACAATCAAAAGAGCAAGGATGGCCAAAGAACAAGCTTCAAGAAGGTAGTAGGCAAGGGGATTTCCAAGGATCCCATACCACCATCTGAAAAAGTAGTTTTGAAACATCAAGCTGTGCAGGGCAAGAAAGATACCCAGGTTTTGTTTAATAACGTCATTGCGGAAACAGCGAGAAAACTCGTTAGAACCCGGAAGAGTAAGGTCAAGGCCTTGGTAGGGGCATTTGAAAAAGTGATCTCACTCCAAGACAAGAAACCTTCTCTAAGAACCATTGCTTGA

mRNA sequence

ATGATTGATGGTGGTAATTTGAGAAGATATTCTATGGGAAAAGAAAGCTCATTGAGTATTGATGAACAAAGTATTTCTCGTCAACGCAGATCCTCTACCGGTTCTTGCCATGATGTGTGCAAGTATGGACATAAACATTCATTCGAAACGAAGGCCAGAGATCCCCTACCGAAAAGAGCAATGAAAAAGCCACTTGATGGCCAAAATTCAGACCAGGTTGTAGCTGTGCTCAAGAGAGAGAAGCCCACTGTTCGCGTGACCAAGTTGAAGGCTTCACCTGATTCAGGGACATGTAGTACTGGTGGCACCAACATGGTGAAACGGGTAGTGCCTATAAATTCTCCTATCACGCAAAGTCCAGTAGAGATTGAAGTTATGAATGAAAGTAAGGAACTCATAGTGCCCGTGAATTCTCCTAGCAGGCGGAGTGCAGTAGAGATTGAAGTTATGAATGGAAGTAAGAAATTCGTAGTGCCCGTCAATTCTCCTAACGGGCGAAGTCCAGTAGAGATTGAAGTTATGAATGAAACTAAGGAACTGGTGGTGCCTGTAAATTCTCCTACCAAGCGAAGTCCAGTTGGGATTGAAGTTATGAATGATAAGGAACTGGTAGTGCCTGTAGATTCTCCTACCAGGCGAAGTCCAGTAGAGAATGAAGTTATGAATGAAAGTAAGGAACTGGTGGACAAGACAAAAACCCATAAACCCAAAATTCATAAAACTACAAAGCAAGTAGTTATTCTGTCTACAAAATCTGAAAGTTCGCCAAAACAAGCCTTGACCAATACTGGAAAAGCCAAATTTCCCAAAAGGCTCGACAGTTTGTTGAAGCCGAATTCTTCAAAATTAAAATCAGTGACCTCTTCTGGTTTTTCTGGGAATATTGCAGTCCACAGAAACAACAGTTCTAAACCAGGTGAAGAAGGGAGGACCTCGAAAGGAATTGGAACGAAAGTAGCAGGAAATTCAGTAGTCATGTCAGGTGCAAAACCTGTTGATGCTGTTGCAAACTTACCTGCAATAAAGAACGAGAACTCGAAAGTTGTCTCTCGTGTTGTAAGTCAGAACAAAACAAGAAGAGCTCAAACCAAGGATGCCCCGAATGTGGAATTACGGAAGAAAACCTTGCATGTCAGCGATTCTGAAACTAAGAAGGCCAAGGTTGTAGAATCAGACCAAAATATAAAGCCTGCTTTGAAGCTGTGTCCAAAGCCACCATCATCACCATACAAGTCATCATCCTTAGCAGATTGCCCATCTCTTTCGCCTCGTAAAGAAAACTGTGGCGAAACTAAATATAAAAGAAGTGAAGCAAATGCCACTTTTTCGAGACCCAACAAACAAGGTGGCATAAAAAAAGAAGAGGCTCACAATGGGAATAAAAAAGGCAGGTCCCCGAGAATGCTTCCAACCAAAGGAAAGGATTCTTCATCTTTGAACTTAAATTTCAGGAACGGAAAGGTAGTCAACCTTCACTCCGAAAGTCCTAGTGCAAGGAGGCTCAAATTTATGCGAGGAAGATCGCTCGGGGACAATCAAAAGAGCAAGGATGGCCAAAGAACAAGCTTCAAGAAGGTAGTAGGCAAGGGGATTTCCAAGGATCCCATACCACCATCTGAAAAAGTAGTTTTGAAACATCAAGCTGTGCAGGGCAAGAAAGATACCCAGGTTTTGTTTAATAACGTCATTGCGGAAACAGCGAGAAAACTCGTTAGAACCCGGAAGAGTAAGGTCAAGGCCTTGGTAGGGGCATTTGAAAAAGTGATCTCACTCCAAGACAAGAAACCTTCTCTAAGAACCATTGCTTGA

Coding sequence (CDS)

ATGATTGATGGTGGTAATTTGAGAAGATATTCTATGGGAAAAGAAAGCTCATTGAGTATTGATGAACAAAGTATTTCTCGTCAACGCAGATCCTCTACCGGTTCTTGCCATGATGTGTGCAAGTATGGACATAAACATTCATTCGAAACGAAGGCCAGAGATCCCCTACCGAAAAGAGCAATGAAAAAGCCACTTGATGGCCAAAATTCAGACCAGGTTGTAGCTGTGCTCAAGAGAGAGAAGCCCACTGTTCGCGTGACCAAGTTGAAGGCTTCACCTGATTCAGGGACATGTAGTACTGGTGGCACCAACATGGTGAAACGGGTAGTGCCTATAAATTCTCCTATCACGCAAAGTCCAGTAGAGATTGAAGTTATGAATGAAAGTAAGGAACTCATAGTGCCCGTGAATTCTCCTAGCAGGCGGAGTGCAGTAGAGATTGAAGTTATGAATGGAAGTAAGAAATTCGTAGTGCCCGTCAATTCTCCTAACGGGCGAAGTCCAGTAGAGATTGAAGTTATGAATGAAACTAAGGAACTGGTGGTGCCTGTAAATTCTCCTACCAAGCGAAGTCCAGTTGGGATTGAAGTTATGAATGATAAGGAACTGGTAGTGCCTGTAGATTCTCCTACCAGGCGAAGTCCAGTAGAGAATGAAGTTATGAATGAAAGTAAGGAACTGGTGGACAAGACAAAAACCCATAAACCCAAAATTCATAAAACTACAAAGCAAGTAGTTATTCTGTCTACAAAATCTGAAAGTTCGCCAAAACAAGCCTTGACCAATACTGGAAAAGCCAAATTTCCCAAAAGGCTCGACAGTTTGTTGAAGCCGAATTCTTCAAAATTAAAATCAGTGACCTCTTCTGGTTTTTCTGGGAATATTGCAGTCCACAGAAACAACAGTTCTAAACCAGGTGAAGAAGGGAGGACCTCGAAAGGAATTGGAACGAAAGTAGCAGGAAATTCAGTAGTCATGTCAGGTGCAAAACCTGTTGATGCTGTTGCAAACTTACCTGCAATAAAGAACGAGAACTCGAAAGTTGTCTCTCGTGTTGTAAGTCAGAACAAAACAAGAAGAGCTCAAACCAAGGATGCCCCGAATGTGGAATTACGGAAGAAAACCTTGCATGTCAGCGATTCTGAAACTAAGAAGGCCAAGGTTGTAGAATCAGACCAAAATATAAAGCCTGCTTTGAAGCTGTGTCCAAAGCCACCATCATCACCATACAAGTCATCATCCTTAGCAGATTGCCCATCTCTTTCGCCTCGTAAAGAAAACTGTGGCGAAACTAAATATAAAAGAAGTGAAGCAAATGCCACTTTTTCGAGACCCAACAAACAAGGTGGCATAAAAAAAGAAGAGGCTCACAATGGGAATAAAAAAGGCAGGTCCCCGAGAATGCTTCCAACCAAAGGAAAGGATTCTTCATCTTTGAACTTAAATTTCAGGAACGGAAAGGTAGTCAACCTTCACTCCGAAAGTCCTAGTGCAAGGAGGCTCAAATTTATGCGAGGAAGATCGCTCGGGGACAATCAAAAGAGCAAGGATGGCCAAAGAACAAGCTTCAAGAAGGTAGTAGGCAAGGGGATTTCCAAGGATCCCATACCACCATCTGAAAAAGTAGTTTTGAAACATCAAGCTGTGCAGGGCAAGAAAGATACCCAGGTTTTGTTTAATAACGTCATTGCGGAAACAGCGAGAAAACTCGTTAGAACCCGGAAGAGTAAGGTCAAGGCCTTGGTAGGGGCATTTGAAAAAGTGATCTCACTCCAAGACAAGAAACCTTCTCTAAGAACCATTGCTTGA

Protein sequence

MIDGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMKKPLDGQNSDQVVAVLKREKPTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPVEIEVMNESKELIVPVNSPSRRSAVEIEVMNGSKKFVVPVNSPNGRSPVEIEVMNETKELVVPVNSPTKRSPVGIEVMNDKELVVPVDSPTRRSPVENEVMNESKELVDKTKTHKPKIHKTTKQVVILSTKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGEEGRTSKGIGTKVAGNSVVMSGAKPVDAVANLPAIKNENSKVVSRVVSQNKTRRAQTKDAPNVELRKKTLHVSDSETKKAKVVESDQNIKPALKLCPKPPSSPYKSSSLADCPSLSPRKENCGETKYKRSEANATFSRPNKQGGIKKEEAHNGNKKGRSPRMLPTKGKDSSSLNLNFRNGKVVNLHSESPSARRLKFMRGRSLGDNQKSKDGQRTSFKKVVGKGISKDPIPPSEKVVLKHQAVQGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEKVISLQDKKPSLRTIA
Homology
BLAST of Sgr021002 vs. NCBI nr
Match: XP_022144538.1 (flocculation protein FLO11-like isoform X1 [Momordica charantia] >XP_022144539.1 flocculation protein FLO11-like isoform X1 [Momordica charantia])

HSP 1 Score: 594.3 bits (1531), Expect = 1.1e-165
Identity = 415/894 (46.42%), Postives = 477/894 (53.36%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DGGNLRR+SMGK S   ID+Q  S  RRSSTGSCHD CKYGHKHS ETKAR PL KRAMK
Sbjct: 16  DGGNLRRFSMGKASLSGIDDQISS--RRSSTGSCHDFCKYGHKHSLETKARVPLLKRAMK 75

Query: 63  KPLDGQNSDQVVAVLKREK-PTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPV 122
           K L+GQNSD VVA+ K+ K P V VTKLK SPD GTC TGG ++VKRVVP+NSP  +SPV
Sbjct: 76  KSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVPVNSPARRSPV 135

Query: 123 E------------------------IEVMNESKELIVPVNSPSRRSAVEIEVMNGSKKFV 182
           E                        IE+MNE+KE + PV SPSRRS VEIEVMN SK+ V
Sbjct: 136 EIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEIEVMNESKEQV 195

Query: 183 VPVNSPNGRSPVEIEVMNETKELVVPVN-------------------------------- 242
           VPVNS + +SP EIEVMNE+K+ VVPVN                                
Sbjct: 196 VPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAAVTSPSRRSSV 255

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 256 GVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEVMNENKERV 315

Query: 303 ------------------------------------------------------------ 362
                                                                       
Sbjct: 316 AAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVPVNSSTRRISL 375

Query: 363 ----------------SPTKRSPVGIEVMND-------------------------KELV 422
                           SP++RSPV IEVMN+                         K+ V
Sbjct: 376 GIEVMKENKERVAAVTSPSRRSPVKIEVMNESKERVVPINSSSRQCPVDIEVVNESKKRV 435

Query: 423 VPVDSPTRR------------------------------------------------SPV 482
           VPV+S TRR                                                SP 
Sbjct: 436 VPVNSSTRRSSLVIEAMNENKERVAALASSSRQSPVEIEVINESKEQVVPVTSSSMQSPA 495

Query: 483 ENEVMNESKELV----------------------------DKTKTHKPKIHKTTKQVVIL 542
           E EVMNESKELV                               KTHKPKIH TTKQVV  
Sbjct: 496 ETEVMNESKELVVPVNSPSRQNPSKIEVTKERKKPLVKAKTSPKTHKPKIHLTTKQVVFS 555

Query: 543 STKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGEE 602
             KS +SPKQAL N G+ +  KRL+SLLKP + K KS+ SSG  GNIAVHR++SS+ GE 
Sbjct: 556 PRKSANSPKQALINNGEVRVSKRLNSLLKPKTLKEKSMISSGSFGNIAVHRHDSSETGEG 615

BLAST of Sgr021002 vs. NCBI nr
Match: XP_022144540.1 (flocculation protein FLO11-like isoform X2 [Momordica charantia])

HSP 1 Score: 589.0 bits (1517), Expect = 4.8e-164
Identity = 412/889 (46.34%), Postives = 473/889 (53.21%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DGGNLRR+SMGK S   ID+Q  S  RRSSTGSCHD CKYGHKHS ETKAR PL KRAMK
Sbjct: 16  DGGNLRRFSMGKASLSGIDDQISS--RRSSTGSCHDFCKYGHKHSLETKARVPLLKRAMK 75

Query: 63  KPLDGQNSDQVVAVLKREK-PTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPV 122
           K L+GQNSD VVA+ K+ K P V VTKLK SPD GTC TGG ++VKRVVP+NSP  +SPV
Sbjct: 76  KSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVPVNSPARRSPV 135

Query: 123 E------------------------IEVMNESKELIVPVNSPSRRSAVEIEVMNGSKKFV 182
           E                        IE+MNE+KE + PV SPSRRS VEIEVMN SK+ V
Sbjct: 136 EIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEIEVMNESKEQV 195

Query: 183 VPVNSPNGRSPVEIEVMNETKELVVPVN-------------------------------- 242
           VPVNS + +SP EIEVMNE+K+ VVPVN                                
Sbjct: 196 VPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAAVTSPSRRSSV 255

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 256 GVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEVMNENKERV 315

Query: 303 ------------------------------------------------------------ 362
                                                                       
Sbjct: 316 AAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVPVNSSTRRISL 375

Query: 363 ----------------SPTKRSPVGIEVMND-------------------------KELV 422
                           SP++RSPV IEVMN+                         K+ V
Sbjct: 376 GIEVMKENKERVAAVTSPSRRSPVKIEVMNESKERVVPINSSSRQCPVDIEVVNESKKRV 435

Query: 423 VPVDSPTRR------------------------------------------------SPV 482
           VPV+S TRR                                                SP 
Sbjct: 436 VPVNSSTRRSSLVIEAMNENKERVAALASSSRQSPVEIEVINESKEQVVPVTSSSMQSPA 495

Query: 483 ENEVMNESKELV----------------------------DKTKTHKPKIHKTTKQVVIL 542
           E EVMNESKELV                               KTHKPKIH TTKQVV  
Sbjct: 496 ETEVMNESKELVVPVNSPSRQNPSKIEVTKERKKPLVKAKTSPKTHKPKIHLTTKQVVFS 555

Query: 543 STKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGEE 598
             KS +SPKQAL N G+ +  KRL+SLLKP + K KS+ SSG  GNIAVHR++SS+ GE 
Sbjct: 556 PRKSANSPKQALINNGEVRVSKRLNSLLKPKTLKEKSMISSGSFGNIAVHRHDSSETGEG 615

BLAST of Sgr021002 vs. NCBI nr
Match: KAG6607113.1 (hypothetical protein SDJN03_00455, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587.0 bits (1512), Expect = 1.8e-163
Identity = 392/749 (52.34%), Postives = 460/749 (61.42%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DG  LRR SMGK  SLSIDEQ+  R RRSS GSCHD+CKYGH HS ETKAR PL KRAMK
Sbjct: 18  DGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHNHSLETKARVPLLKRAMK 77

Query: 63  KPLDGQNSDQVVAVLKREKPTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPVE 122
           K LD QNSDQ V V+  +K +VRVTK K SP SGTC +GGT+++KRVVPI+SP ++ PVE
Sbjct: 78  KALDAQNSDQAV-VVPEKKHSVRVTKSKVSPSSGTCISGGTDVIKRVVPISSP-SRRPVE 137

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 138 TGVASKSKEQATLVKAPNRQSETEVTSKSKEQATLVKAPNGQSETEVTSESKEPEVPVGS 197

Query: 183 -------------------IEVMNESKELIVPVNSPSRRSAV----------------EI 242
                              IEV++ESKELIVPVNSP+R+  V                +I
Sbjct: 198 PSKKIEVIMVLPVNSPTKRIEVISESKELIVPVNSPTRQIEVISESKELAVPLNSPTRQI 257

Query: 243 EVMNGSKKFVVPVNSPNGRSPVEIEVMNETKELVVPVNSPTKRSPVGIEVMNDK------ 302
           EV++ SK+ VVP+NSP      +IEV++E++ELVVPVNSPT++SPV +EV ++       
Sbjct: 258 EVISESKELVVPLNSPTR----QIEVISESQELVVPVNSPTRQSPVEMEVSSESSERVVP 317

Query: 303 -------------------ELVVPVDSPT------------------------------R 362
                              E VVP  SP+                              R
Sbjct: 318 ENSTSRHSSVEIEATSKNIERVVPETSPSGRSRVEIGVRSEIKEPVVVAESSLSRQGSNR 377

Query: 363 RSPVENEVMNESKELVDKTK----THKPKIHKTTKQVVILSTKSESSPKQALTNTGKAKF 422
           RSP+++E MNE KE V KTK    T KPK HKTTKQVV  STKSESSPKQAL +  +A  
Sbjct: 378 RSPLKSEAMNEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALASVAEAGG 437

Query: 423 PKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGEEGRTSKGIGTKVAGNSVVMSG 482
               DSL KP + K KSVTSSG SGNIA H NN+SK  E   T KG G KV   S++ + 
Sbjct: 438 ----DSLSKPKALKAKSVTSSGPSGNIAAHSNNNSKSCEGAGTLKGNGIKVVEKSIITTD 497

Query: 483 AKPVDAVANLPAIKNENSKVVSRVVSQNKTRRAQTKDAPNVELRKKTLHVSDSETKKAKV 542
              VD V NLPAIKN+NSKVVS+V SQNKTRRAQ K+A +VE ++K LHV + ETKK K+
Sbjct: 498 PNSVDTVVNLPAIKNKNSKVVSQVKSQNKTRRAQAKEASSVESQEKILHVINVETKKTKL 557

Query: 543 VESDQNIKPALKLCPKPPSSPYKSSSLADCPSLSPRKENCGETKYKRSEANATFSRPNKQ 598
           VESDQN K  LK   K P      +SLA+ PSLSP +E+ G TKY + EANAT S   K 
Sbjct: 558 VESDQNDKHGLKGYQKSP------TSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKH 617

BLAST of Sgr021002 vs. NCBI nr
Match: XP_023523723.1 (uncharacterized protein LOC111787871 [Cucurbita pepo subsp. pepo] >XP_023523724.1 uncharacterized protein LOC111787871 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.0 bits (1512), Expect = 1.8e-163
Identity = 399/779 (51.22%), Postives = 465/779 (59.69%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DG  LRR SMGK  SLSIDEQ+  R RRSS GSCHD+CKYGH HS ETKAR PL KRAMK
Sbjct: 18  DGDRLRRLSMGKAISLSIDEQNSFRDRRSSIGSCHDICKYGHNHSLETKARVPLLKRAMK 77

Query: 63  KPLDGQNSDQVVAVLKREKPTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPVE 122
           K LD QNSDQ V V+  +K +VRVTK K SP SGTC +GGT+++KRVVPI+SP ++ PVE
Sbjct: 78  KALDAQNSDQAV-VVPEKKHSVRVTKSKVSPSSGTCISGGTDVIKRVVPISSP-SRRPVE 137

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 138 TGVKSKSKEQATLVKAPNRQSETEVTSKSKEQATVVKAPNGQSETEVTSESKEPEVPMGS 197

Query: 183 ----IEVMNESKELIVPVNSPSRRSAV----------------EIEVMNGSKKFVVPVNS 242
               IEV++E+K L+VP+NSP+++  V                 IEV++ SK+ +VPVNS
Sbjct: 198 PTKQIEVISENKALVVPMNSPTKKIEVISESNELVLPVNSPTKRIEVISESKELIVPVNS 257

Query: 243 PNGRSPV----------------EIEVMNETKELVVPVNSPTKRSPVGIEVMNDKELVVP 302
           P  +  V                +IEV++E+KELVVP+NSPT+R  V   +   KELVVP
Sbjct: 258 PTKQIEVISESKELVVPLHSPTRQIEVISESKELVVPLNSPTRRIEV---ICESKELVVP 317

Query: 303 VDSPTRRSPVENEV---------------------------------------------- 362
           V+SPTR+SPVE EV                                              
Sbjct: 318 VNSPTRQSPVEMEVSSESSERVVPENSTSRHSSVEIEATSKNMEQVTPETFPSRRSRVEI 377

Query: 363 --------------------------------MNESKELVDKTK----THKPKIHKTTKQ 422
                                           MNE KELV KTK    T KPK HKTTKQ
Sbjct: 378 GVRRESKEPVVVAESSLSRQGSNRRSPLKSEAMNEGKELVAKTKTKPITSKPKFHKTTKQ 437

Query: 423 VVILSTKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSK 482
           VV  STKSESSPKQALT+  +A      DSL KP + K KSVTSSG SGNIA H NN SK
Sbjct: 438 VVYSSTKSESSPKQALTSVAEAGG----DSLSKPKALKAKSVTSSGPSGNIAAHSNNISK 497

Query: 483 PGEEGRTSKGIGTKVAGNSVVMSGAKPVDAVANLPAIKNENSKVVSRVVSQNKTRRAQTK 542
            GE   T KG GTKV   S++      VD V NLPAIKN+NSKVVS+V SQNKTRRAQ K
Sbjct: 498 SGEGAGTLKGNGTKVVEKSIITMDPNSVDTVVNLPAIKNKNSKVVSQVKSQNKTRRAQAK 557

Query: 543 DAPNVELRKKTLHVSDSETKKAKVVESDQNIKPALKLCPKPPSSPYKSSSLADCPSLSPR 602
           +A +VE ++K LHV + ETKK+K++ESDQN K  LK   K P      SSLA+ PSLSP 
Sbjct: 558 EASSVESQEKILHVINVETKKSKLLESDQNDKHGLKGYQKSP------SSLANGPSLSPL 617

BLAST of Sgr021002 vs. NCBI nr
Match: KAG7036803.1 (hypothetical protein SDJN02_00423, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 580.1 bits (1494), Expect = 2.2e-161
Identity = 395/785 (50.32%), Postives = 462/785 (58.85%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DG  LRR SMGK  SLSIDEQ+  R RRSS GSCHD+CKYGH HS ETKAR PL KRAMK
Sbjct: 18  DGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHNHSLETKARVPLLKRAMK 77

Query: 63  KPLDGQNSDQVVAVLKREKPTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPVE 122
           K LD QNSDQ V V+  +K +VRVTK K SP  GTC +GGT+++KRVVPI+SP ++ PVE
Sbjct: 78  KALDAQNSDQAV-VVPEKKHSVRVTKSKVSPSPGTCISGGTDVIKRVVPISSP-SRRPVE 137

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 138 TGVTSKSKEQATLVKAPNRQSETEVTSKSKEQATLVKAPNGQSETEVTSESKEPEVPVGS 197

Query: 183 -----------------------------------------------------------I 242
                                                                      I
Sbjct: 198 PTKQIEDTSESKEPEVPVGSPTKQIEVISENKALVVPMNSPSKKIEVIMVLPVNSPTKRI 257

Query: 243 EVMNESKELIVPVNSPSRRSAV----------------EIEVMNGSKKFVVPVNSPNGRS 302
           EV++ESKELIVPVNSP+R+  V                +IEV++ SK+ VVP+NSP    
Sbjct: 258 EVISESKELIVPVNSPTRQIEVISESKELAVPLNSPTRQIEVISESKELVVPLNSPTR-- 317

Query: 303 PVEIEVMNETKELVVPVNSPTKRSPVGIEVMNDK-------------------------E 362
             +IEV++E++ELVVPVNSPT++SPV +EV ++                          E
Sbjct: 318 --QIEVISESQELVVPVNSPTRQSPVEMEVSSESSERVVPENSTSRHSSVEIEATSKNME 377

Query: 363 LVVPVDSPT--------------------------RRSPVENEVMNESKELVDKTK---- 422
            VVP  SP+                          RRSP+++E MNE KE V KTK    
Sbjct: 378 QVVPETSPSGRSRVEIGVKEPVVVAESSLSRQGSNRRSPLKSEAMNEGKEQVAKTKTKPI 437

Query: 423 THKPKIHKTTKQVVILSTKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFS 482
           T KPK HKTTKQVV  STKSESSPKQALT+  +A      DSL KP + K KSVTSSG S
Sbjct: 438 TSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGG----DSLSKPKALKAKSVTSSGPS 497

Query: 483 GNIAVHRNNSSKPGEEGRTSKGIGTKVAGNSVVMSGAKPVDAVANLPAIKNENSKVVSRV 542
           GNIA H NNSSK GE   T KG GTKV   S++ +    VD V NLPAIKN+NSKVVS+V
Sbjct: 498 GNIAAHSNNSSKSGEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAIKNKNSKVVSQV 557

Query: 543 VSQNKTRRAQTKDAPNVELRKKTLHVSDSETKKAKVVESDQNIKPALKLCPKPPSSPYKS 598
            SQNKTRRAQ K+A +VE ++K LHV + ETKK K+VESDQN K  LK   K P      
Sbjct: 558 KSQNKTRRAQAKEASSVESQEKILHVINVETKKTKLVESDQNDKHGLKGYQKSP------ 617

BLAST of Sgr021002 vs. ExPASy TrEMBL
Match: A0A6J1CSL1 (flocculation protein FLO11-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014195 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 5.5e-166
Identity = 415/894 (46.42%), Postives = 477/894 (53.36%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DGGNLRR+SMGK S   ID+Q  S  RRSSTGSCHD CKYGHKHS ETKAR PL KRAMK
Sbjct: 16  DGGNLRRFSMGKASLSGIDDQISS--RRSSTGSCHDFCKYGHKHSLETKARVPLLKRAMK 75

Query: 63  KPLDGQNSDQVVAVLKREK-PTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPV 122
           K L+GQNSD VVA+ K+ K P V VTKLK SPD GTC TGG ++VKRVVP+NSP  +SPV
Sbjct: 76  KSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVPVNSPARRSPV 135

Query: 123 E------------------------IEVMNESKELIVPVNSPSRRSAVEIEVMNGSKKFV 182
           E                        IE+MNE+KE + PV SPSRRS VEIEVMN SK+ V
Sbjct: 136 EIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEIEVMNESKEQV 195

Query: 183 VPVNSPNGRSPVEIEVMNETKELVVPVN-------------------------------- 242
           VPVNS + +SP EIEVMNE+K+ VVPVN                                
Sbjct: 196 VPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAAVTSPSRRSSV 255

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 256 GVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEVMNENKERV 315

Query: 303 ------------------------------------------------------------ 362
                                                                       
Sbjct: 316 AAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVPVNSSTRRISL 375

Query: 363 ----------------SPTKRSPVGIEVMND-------------------------KELV 422
                           SP++RSPV IEVMN+                         K+ V
Sbjct: 376 GIEVMKENKERVAAVTSPSRRSPVKIEVMNESKERVVPINSSSRQCPVDIEVVNESKKRV 435

Query: 423 VPVDSPTRR------------------------------------------------SPV 482
           VPV+S TRR                                                SP 
Sbjct: 436 VPVNSSTRRSSLVIEAMNENKERVAALASSSRQSPVEIEVINESKEQVVPVTSSSMQSPA 495

Query: 483 ENEVMNESKELV----------------------------DKTKTHKPKIHKTTKQVVIL 542
           E EVMNESKELV                               KTHKPKIH TTKQVV  
Sbjct: 496 ETEVMNESKELVVPVNSPSRQNPSKIEVTKERKKPLVKAKTSPKTHKPKIHLTTKQVVFS 555

Query: 543 STKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGEE 602
             KS +SPKQAL N G+ +  KRL+SLLKP + K KS+ SSG  GNIAVHR++SS+ GE 
Sbjct: 556 PRKSANSPKQALINNGEVRVSKRLNSLLKPKTLKEKSMISSGSFGNIAVHRHDSSETGEG 615

BLAST of Sgr021002 vs. ExPASy TrEMBL
Match: A0A6J1CRX0 (flocculation protein FLO11-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014195 PE=4 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 2.3e-164
Identity = 412/889 (46.34%), Postives = 473/889 (53.21%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DGGNLRR+SMGK S   ID+Q  S  RRSSTGSCHD CKYGHKHS ETKAR PL KRAMK
Sbjct: 16  DGGNLRRFSMGKASLSGIDDQISS--RRSSTGSCHDFCKYGHKHSLETKARVPLLKRAMK 75

Query: 63  KPLDGQNSDQVVAVLKREK-PTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPV 122
           K L+GQNSD VVA+ K+ K P V VTKLK SPD GTC TGG ++VKRVVP+NSP  +SPV
Sbjct: 76  KSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVPVNSPARRSPV 135

Query: 123 E------------------------IEVMNESKELIVPVNSPSRRSAVEIEVMNGSKKFV 182
           E                        IE+MNE+KE + PV SPSRRS VEIEVMN SK+ V
Sbjct: 136 EIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEIEVMNESKEQV 195

Query: 183 VPVNSPNGRSPVEIEVMNETKELVVPVN-------------------------------- 242
           VPVNS + +SP EIEVMNE+K+ VVPVN                                
Sbjct: 196 VPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAAVTSPSRRSSV 255

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 256 GVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEVMNENKERV 315

Query: 303 ------------------------------------------------------------ 362
                                                                       
Sbjct: 316 AAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVPVNSSTRRISL 375

Query: 363 ----------------SPTKRSPVGIEVMND-------------------------KELV 422
                           SP++RSPV IEVMN+                         K+ V
Sbjct: 376 GIEVMKENKERVAAVTSPSRRSPVKIEVMNESKERVVPINSSSRQCPVDIEVVNESKKRV 435

Query: 423 VPVDSPTRR------------------------------------------------SPV 482
           VPV+S TRR                                                SP 
Sbjct: 436 VPVNSSTRRSSLVIEAMNENKERVAALASSSRQSPVEIEVINESKEQVVPVTSSSMQSPA 495

Query: 483 ENEVMNESKELV----------------------------DKTKTHKPKIHKTTKQVVIL 542
           E EVMNESKELV                               KTHKPKIH TTKQVV  
Sbjct: 496 ETEVMNESKELVVPVNSPSRQNPSKIEVTKERKKPLVKAKTSPKTHKPKIHLTTKQVVFS 555

Query: 543 STKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGEE 598
             KS +SPKQAL N G+ +  KRL+SLLKP + K KS+ SSG  GNIAVHR++SS+ GE 
Sbjct: 556 PRKSANSPKQALINNGEVRVSKRLNSLLKPKTLKEKSMISSGSFGNIAVHRHDSSETGEG 615

BLAST of Sgr021002 vs. ExPASy TrEMBL
Match: A0A6J1K8S1 (muscle M-line assembly protein unc-89-like isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC111492710 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 3.1e-161
Identity = 397/757 (52.44%), Postives = 461/757 (60.90%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DG  LRR SMGK  SLSIDEQ+  R RRSS GSCHD+CKYGH HS ETKAR PL KRAMK
Sbjct: 18  DGDRLRRLSMGKAISLSIDEQNSFRDRRSSIGSCHDICKYGHNHSLETKARVPLLKRAMK 77

Query: 63  KPLDGQNSDQVVAVLKREKPTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSP------- 122
           K LD QNSDQ V V+  +K +V VTK K SP S TC +GGT+++KRVVPI+SP       
Sbjct: 78  KALDAQNSDQAV-VVPEKKHSVCVTKSKVSPSSKTCISGGTDVIKRVVPISSPSRRLVET 137

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 138 GVMSKSKEQATLVKAPNGQSETEVTSESKEPEAPVGSPTKQIEVISENKELVVPMNSPTK 197

Query: 183 ----ITQS-----PV-----EIEVMNESKELIVPVNSPSRRSAV---------------- 242
               I++S     PV      IEV++ESKELIVPVNSP+R+  V                
Sbjct: 198 KIEVISESNEVVLPVNSPTKRIEVISESKELIVPVNSPTRQIEVISESKELVMPLNSPTR 257

Query: 243 EIEVMNGSKKFVVPVNSPNGRSPVEIEVMNETKELVVPVNSPTKRSPVGIEVMNDK---- 302
           +IEV++GSK+ VVP+NSP      +IEV++E+KELVVPVNSPT++SPV +EV ++     
Sbjct: 258 QIEVISGSKELVVPLNSPTR----QIEVISESKELVVPVNSPTRQSPVEMEVSSESSERV 317

Query: 303 ---------------------ELVVPVDSPT----------------------------- 362
                                E VVP   P+                             
Sbjct: 318 VPENSTSRHSSVEIEATSKNMERVVPETFPSRRSRVEIGVGSESKEPVVVAESSLRRQGS 377

Query: 363 -RRSPVENEVMNESKELVDKTK----THKPKIHKTTKQVVILSTKSESSPKQALTNTGKA 422
            RRSP+++E MNE KELV KTK    T KPK HKTTKQVV  STKSESSPKQALT+  +A
Sbjct: 378 NRRSPLKSEAMNEGKELVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEA 437

Query: 423 KFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGEEGRTSKGIGTKVAGNSVVM 482
                 DSLLKP + K KSV SSG SGNIA H NNSSK GE   T KG G KV   S++ 
Sbjct: 438 GG----DSLLKPKAFKEKSVISSGSSGNIAAHSNNSSKSGEGAGTLKGNGMKVVEKSIIT 497

Query: 483 SGAKPVDAVANLPAIKNENSKVVSRVVSQNKTRRAQTKDAPNVELRKKTLHVSDSETKKA 542
                VD V NLPAIK +NSK VS+V SQNKTRR Q K+A +VE ++K LHV + ETKK 
Sbjct: 498 MDPNSVDTVVNLPAIK-KNSKAVSQVKSQNKTRRIQAKEASSVESQEKILHVINVETKKN 557

Query: 543 KVVESDQNIKPALKLCPKPPSSPYKSSSLADCPSLSPRKENCGETKYKRSEANATFSRPN 602
           K+VESDQN K  LK   K P      SSL + PSLSP +E+ G TKY + EANAT S   
Sbjct: 558 KLVESDQNDKHGLKGYQKSP------SSLTNGPSLSPLREDSGGTKYTKYEANATVSGSK 617

BLAST of Sgr021002 vs. ExPASy TrEMBL
Match: A0A6J1GBV9 (muscle M-line assembly protein unc-89-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452563 PE=4 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 4.1e-161
Identity = 392/769 (50.98%), Postives = 460/769 (59.82%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DG  LRR SMGK  SLSIDEQ+  R RRSS GSCHD+CKYGH HS ETKAR PL KRAMK
Sbjct: 18  DGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHNHSLETKARVPLLKRAMK 77

Query: 63  KPLDGQNSDQVVAVLKREKPTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPVE 122
           K LD QNSDQ V V+  +K +VRVTK K SP SGTC +GGT+++KRVVPI+SP ++ PVE
Sbjct: 78  KALDAQNSDQAV-VVPEKKHSVRVTKSKVSPSSGTCISGGTDVIKRVVPISSP-SRRPVE 137

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 138 TGVTSKSKEQATLVKAPNRQSETEVTSKSKEQATLVKAPNGQSETEVTSESKEPEVPVGS 197

Query: 183 ---------------------------------------IEVMNESKELIVPVNSPSRRS 242
                                                  IEV++ESKELIVPVNSP+R+ 
Sbjct: 198 PTKQIEVISENKALVVPMNSPSKKIEVIMVLPVNSPTKRIEVISESKELIVPVNSPTRQI 257

Query: 243 AV----------------EIEVMNGSKKFVVPVNSPNGRSPVEIEVMNETKELVVPVNSP 302
            V                +IEV++ SK+ VVP+NSP      +IEV++E++ELVVPVNSP
Sbjct: 258 EVISESKELAVPLNSPTRQIEVISESKELVVPLNSPTR----QIEVISESQELVVPVNSP 317

Query: 303 TKRSPVGIEVMNDK-------------------------ELVVPVDSPT----------- 362
           T++SPV +EV ++                          E VVP  SP+           
Sbjct: 318 TRQSPVEMEVSSESSERVVPENSTSRHSSVEIEATSKNMERVVPETSPSGRSRVEIGVRS 377

Query: 363 -------------------RRSPVENEVMNESKELVDKTK----THKPKIHKTTKQVVIL 422
                              RRSP+++E MNE KE V KTK    T KPK HKTTKQVV  
Sbjct: 378 EIKEPVVVAESSLSRQGSNRRSPLKSEAMNEGKEQVAKTKTKPITSKPKFHKTTKQVVYS 437

Query: 423 STKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGEE 482
           STKSESSPKQAL +  +A      DSL KP + K KSVTSSG SGNIA H NN+SK  E 
Sbjct: 438 STKSESSPKQALASVAEAGG----DSLSKPKALKAKSVTSSGPSGNIAAHSNNNSKSCEG 497

Query: 483 GRTSKGIGTKVAGNSVVMSGAKPVDAVANLPAIKNENSKVVSRVVSQNKTRRAQTKDAPN 542
             T KG GTKV   S++ +    VD V NLPAIKN+NSKVVS+V SQNKTRRAQ K+A +
Sbjct: 498 AGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAIKNKNSKVVSQVKSQNKTRRAQAKEASS 557

Query: 543 VELRKKTLHVSDSETKKAKVVESDQNIKPALKLCPKPPSSPYKSSSLADCPSLSPRKENC 598
           VE ++K LHV + ETKK K+VESDQN K  LK   K P      +SLA+ PSLSP +E+ 
Sbjct: 558 VESQEKILHVINVETKKTKLVESDQNDKHGLKGYQKSP------TSLANGPSLSPLREDS 617

BLAST of Sgr021002 vs. ExPASy TrEMBL
Match: A0A6J1KF87 (muscle M-line assembly protein unc-89-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492710 PE=4 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 1.1e-158
Identity = 397/779 (50.96%), Postives = 461/779 (59.18%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DG  LRR SMGK  SLSIDEQ+  R RRSS GSCHD+CKYGH HS ETKAR PL KRAMK
Sbjct: 18  DGDRLRRLSMGKAISLSIDEQNSFRDRRSSIGSCHDICKYGHNHSLETKARVPLLKRAMK 77

Query: 63  KPLDGQNSDQVVAVLKREKPTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSP------- 122
           K LD QNSDQ V V+  +K +V VTK K SP S TC +GGT+++KRVVPI+SP       
Sbjct: 78  KALDAQNSDQAV-VVPEKKHSVCVTKSKVSPSSKTCISGGTDVIKRVVPISSPSRRLVET 137

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 138 GVMSKSKEQATLVKAPNGQSETEVTSKSKEQATLVKAPNGQSETEVTSESKEPEAPVGSP 197

Query: 183 --------------------------ITQS-----PV-----EIEVMNESKELIVPVNSP 242
                                     I++S     PV      IEV++ESKELIVPVNSP
Sbjct: 198 TKQIEVISENKELVVPMNSPTKKIEVISESNEVVLPVNSPTKRIEVISESKELIVPVNSP 257

Query: 243 SRRSAV----------------EIEVMNGSKKFVVPVNSPNGRSPVEIEVMNETKELVVP 302
           +R+  V                +IEV++GSK+ VVP+NSP      +IEV++E+KELVVP
Sbjct: 258 TRQIEVISESKELVMPLNSPTRQIEVISGSKELVVPLNSPTR----QIEVISESKELVVP 317

Query: 303 VNSPTKRSPVGIEVMNDK-------------------------ELVVPVDSPT------- 362
           VNSPT++SPV +EV ++                          E VVP   P+       
Sbjct: 318 VNSPTRQSPVEMEVSSESSERVVPENSTSRHSSVEIEATSKNMERVVPETFPSRRSRVEI 377

Query: 363 -----------------------RRSPVENEVMNESKELVDKTK----THKPKIHKTTKQ 422
                                  RRSP+++E MNE KELV KTK    T KPK HKTTKQ
Sbjct: 378 GVGSESKEPVVVAESSLRRQGSNRRSPLKSEAMNEGKELVAKTKTKPITSKPKFHKTTKQ 437

Query: 423 VVILSTKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSK 482
           VV  STKSESSPKQALT+  +A      DSLLKP + K KSV SSG SGNIA H NNSSK
Sbjct: 438 VVYSSTKSESSPKQALTSVAEAGG----DSLLKPKAFKEKSVISSGSSGNIAAHSNNSSK 497

Query: 483 PGEEGRTSKGIGTKVAGNSVVMSGAKPVDAVANLPAIKNENSKVVSRVVSQNKTRRAQTK 542
            GE   T KG G KV   S++      VD V NLPAIK +NSK VS+V SQNKTRR Q K
Sbjct: 498 SGEGAGTLKGNGMKVVEKSIITMDPNSVDTVVNLPAIK-KNSKAVSQVKSQNKTRRIQAK 557

Query: 543 DAPNVELRKKTLHVSDSETKKAKVVESDQNIKPALKLCPKPPSSPYKSSSLADCPSLSPR 602
           +A +VE ++K LHV + ETKK K+VESDQN K  LK   K P      SSL + PSLSP 
Sbjct: 558 EASSVESQEKILHVINVETKKNKLVESDQNDKHGLKGYQKSP------SSLTNGPSLSPL 617

BLAST of Sgr021002 vs. TAIR 10
Match: AT5G39380.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 118.2 bits (295), Expect = 2.2e-26
Identity = 179/602 (29.73%), Postives = 266/602 (44.19%), Query Frame = 0

Query: 3   DGGNLRRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMK 62
           DG N    S GK  +    E+ I    R+STGSCHD+CKYG +     K      K+  K
Sbjct: 20  DGLNPGGDSTGKAMTSKPKEKKIPHYLRASTGSCHDLCKYGKRQIPVEKPWRSSTKKIFK 79

Query: 63  KPLDGQNSDQVVAVLKREKPTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPVE 122
           K LD   ++                         T   G + M K+V  +         +
Sbjct: 80  KSLDDNLNE-------------------------TLKPGSSKMKKKVREVE--------K 139

Query: 123 IEVMNESKELIVPVNSPSRRSAVEIE---VMNGSKKFVVPVNSPNGRSPVEIEVMNETKE 182
            E  ++S E+I       +R  V+ +   V +G +K  V + S    +PV+ ++  +T  
Sbjct: 140 NEGTDDSFEVI-------KREVVKYQASGVSSGMRKPEVLIISSCDETPVK-QIKKKT-- 199

Query: 183 LVVPVNSPTKRSPVGIEVMNDKELVVPVDSPTRRSPVENEVMNESKELVDKTKTHKPKIH 242
               ++S  K SP                          ++ + S E VD     KPK+ 
Sbjct: 200 ---TLSSKLKPSP--------------------------DLGSRSSENVDAL---KPKVL 259

Query: 243 KTTKQVVILSTKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHR 302
           K +   +  S    +  K   +   K K  KR D   +    K  +V+S   S    V  
Sbjct: 260 KKSYSALTTSKPKVNHEKVVASPVLKPKMGKRNDGKDEDGKVKKGTVSSRVASKKAPVTP 319

Query: 303 NNSSKPGEEGRTSKGIGTKVAGNSVVMSGAKPVDAVANLPAIKNENSKVVSRVVSQNKTR 362
             S  P         +  ++AG+S +          A+  + +N+  + V+R    NK  
Sbjct: 320 RASLSP--------RLSVRLAGSSSLRKSQSL--KAASSSSRQNQKPRPVNRTDEFNK-- 379

Query: 363 RAQTKDAPNVELRKKTLHVSDSETKKAKVVESDQN----IKPALKLCPKPPSSPYKSSSL 422
             Q  D P   + +KTLHV + ET    V E+DQN    ++P L     PP  P +S   
Sbjct: 380 --QLDDYP---VEEKTLHVVEMETTNNVVSENDQNQQGFVEPFL-----PPLPPTQS--- 439

Query: 423 ADCPSLSPRKENCGETKYKRSEANATFSRPNKQGGIKKEE--AHNGNKKGRSPRMLPTKG 482
                 +P+ + C  ++ +  E    ++  + +   ++EE    NG KK R+ R      
Sbjct: 440 ------TPKDDECTVSETEEYE----YTSGSNEAESEEEEIGLSNGEKKPRAARK-EGDS 499

Query: 483 KDSSSLNLNFRNGKVVNLHSESPSARRLKFMRGRSLGDNQKSKDGQ-RTSFKKVVGKGIS 542
            D ++  L FR G +V+  +    AR+LKF RGR LG++ K++D Q R SFKK   + I 
Sbjct: 500 ADEAARKLRFRRGTIVDPDTVGEKARKLKFRRGRGLGED-KAQDAQVRRSFKK--REDIR 506

Query: 543 KDPI-PPSEKVVLKHQAVQGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEKVISL 594
           ++ +    EKVVL+HQ VQ +KD Q LFNNVI ETA KLV  RKSKVKALVGAFE VISL
Sbjct: 560 EEEVNEDGEKVVLRHQDVQ-EKDAQGLFNNVIEETASKLVEARKSKVKALVGAFETVISL 506

BLAST of Sgr021002 vs. TAIR 10
Match: AT5G15430.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 97.1 bits (240), Expect = 5.3e-20
Identity = 164/596 (27.52%), Postives = 231/596 (38.76%), Query Frame = 0

Query: 8   RRYSMGKESSLSIDEQSISRQRRSSTGSCHDVCKYGHKHSFETKARDPLPKRAMKKPLDG 67
           RR S GK S L   E+ +    RS TGSCHD CKYG K   E K R P  KR  +     
Sbjct: 19  RRISTGKLSFLYTQEKVVPNYLRSPTGSCHDACKYGRKDESEDKPRVPHRKRVSRS---- 78

Query: 68  QNSDQVVAVLKREKPTVRVTKLKASPDSGTCSTGGTNMVKRVVPINSPITQSPVEIEVMN 127
                                           +G  N       ++SP+           
Sbjct: 79  -------------------------------FSGAIN-------LDSPL----------- 138

Query: 128 ESKELIVPVNSPSRRSAVEIEVMNGSKKFVVPVNSPNGRSPVEIEVMNETKELVVPVNSP 187
             K L  P+ SPSRR    +   + +K  V   N  +G   V+    + T E VV V+  
Sbjct: 139 RKKALTKPLLSPSRR-CDSVGGFDHAKSQV--RNFSSGVCDVKKSHADGTNEKVVSVS-- 198

Query: 188 TKRSPVGIEVMNDKELVVPVDSPTRRSPVENEVMNESKELVDKTKTHKPKIHKTTKQVVI 247
                                                  L D TK  K K          
Sbjct: 199 ------------------------------------ESRLADSTKRKKKK---------- 258

Query: 248 LSTKSESSPKQALTNTGKAKFPKRLDSLLKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGE 307
                    K    + G+AK  + ++   +  + KLK+V     +  IA+ R+   +   
Sbjct: 259 --------KKTVYVSRGRAK--EIVEQKRRVTALKLKAVAQ---TAEIALRRSTVKRKKM 318

Query: 308 EGRTSKGIGTKVAGNSVVMSGAKPVDAVANLPAIKNENSKVVSRVVSQNKTRRAQTKDAP 367
            G  SK    K A  ++  +          L   K  NS  V       K  R    D  
Sbjct: 319 NG-GSKAAEQKKAVMALRRASMSSKGCSRCLKTKKESNSLSVPL-----KKTRKHVGDKC 378

Query: 368 NVELRKKTLHVSDSETKKAKVVESDQNIKPALKLCPKPPSSPYKSSSLADCPSLSPRKEN 427
              + +KTL+V   ET   ++VES+ N +  +      P    KS    D       K  
Sbjct: 379 KDLVEEKTLYVIKMETVD-EIVESELNQRCVM----DSPIDDPKSEKSQD-------KGE 438

Query: 428 CGETKYKRSEANATFSRPNKQGGIKKEEAHNGNKKGRSPRMLPTKGKDSSSLNLNFRNGK 487
           C ET+++   +        +   +   E  N  ++G+S           +++ L  R GK
Sbjct: 439 CIETEHEDESSQEEEDEEEEDENVSVSEDKNTTREGKSKAFSAESAITGNAMKLRIRRGK 478

Query: 488 VVNLHSESPSARRLKFMRGRSL-GDNQKSKDGQRTSFKKVVGKGISKDPIPPSE-KVVLK 547
           +++  SE  S R+LKF RG+ + G +  SK G R    K  G  +S D     + +VVLK
Sbjct: 499 IIDFGSEGNSPRKLKFKRGKIISGADTTSKSGGRRRL-KTKGTNLSNDKEQQRKPRVVLK 478

Query: 548 HQAVQGKKDTQV-LFNNVIAETARKLVRTRKSKVKALVGAFEKVISLQDKKPSLRT 601
           HQ  + K++++V LFN VI ETA KLV+TRKSKVKALVGAFE VISLQ+K  S  T
Sbjct: 559 HQDTEKKRESRVLLFNKVIKETANKLVQTRKSKVKALVGAFESVISLQEKTSSATT 478

BLAST of Sgr021002 vs. TAIR 10
Match: AT5G61260.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 84.0 bits (206), Expect = 4.6e-16
Identity = 107/375 (28.53%), Postives = 175/375 (46.67%), Query Frame = 0

Query: 229 DKTKTHKPKIHKTTKQVVILSTKSESSPKQALTNTGKAKFPKRLDSLLKPNS--SKLKSV 288
           D  K  K    K+ +  V++  ++ SS +++L +       ++   + KP+S  S  +  
Sbjct: 109 DAVKPWKIARRKSVEGSVVIKVETPSSTRKSLGSVS-----RQSPGITKPDSSVSAKRDA 168

Query: 289 TSSGFSGNIAVHRNNSSKPGEE-GRTSKGIGTK----VAGNSVVMSGAKPVDAVANLPAI 348
            +       +V+  +SSK G E  ++  G+  K       N    SG      V  +PA+
Sbjct: 169 LAVKKKPCASVNSESSSKEGSEIAKSVDGLSVKSNDRARKNKETESGLSGSAVVKKVPAL 228

Query: 349 KNENSKVVSRVVSQNKTRRAQTKDAPNVELRKKTLHVSDSETKKAKVVESDQNIKPALKL 408
           + + S   + V S   ++    K+  NVE  K T  +S  + K+  V   + ++K     
Sbjct: 229 RTDKSSTSTGVGS---SKVCAPKNLKNVEKAKTTQTISGEDVKEKTVCVVESSVKGVKS- 288

Query: 409 CPKPPSSPYKSSSLADCPSLSPRKENCGETKYKRSEANATFSRPNKQGGIKKEEAHNGNK 468
             K PSS  K+    +    +  K     TK    + +   ++  + G     EA+   +
Sbjct: 289 -EKQPSSEKKTMKSGNKSLSTTPKRGSSPTKQIPGKISTGLTKKKETGSADVVEANPKPE 348

Query: 469 KGRSPRMLPTKGKDSSSLNLNFRNGKVVNLHSESPSARRLKFMRGRSLGDNQKSKDGQRT 528
           K   P+   T  K S +  + F+ GKV++   E  S R +KF + R + + +   +G++ 
Sbjct: 349 KKVRPK--KTGVKVSLAQQMTFKKGKVLDPKPEDSSPRWIKFKK-RVVQELKTQSEGKKK 408

Query: 529 SFK-KVVGKGISKDPIPPS--EKVVLKHQAVQGKKDTQVLFNNVIAETARKLVRTRKSKV 588
           + K + +G     D    S  EKVVL+H+ V+GKK    LFNNVI ET  KL + RK KV
Sbjct: 409 NLKDRRLGVETKTDSCEGSKREKVVLRHRKVEGKKKMITLFNNVIEETVNKLTKVRKHKV 468

Query: 589 KALVGAFEKVISLQD 594
           KAL+GAFE VISLQD
Sbjct: 469 KALIGAFETVISLQD 470

BLAST of Sgr021002 vs. TAIR 10
Match: AT5G07820.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 72.8 bits (177), Expect = 1.1e-12
Identity = 116/408 (28.43%), Postives = 175/408 (42.89%), Query Frame = 0

Query: 230 KTKTHKPKIH---KTTKQVVILSTKS-----ESSPKQALTNT------GKAKFPKRLDSL 289
           +TK+  P +    +T K+  ++  K+       SPK+ L+        GK   P R D +
Sbjct: 159 ETKSTSPSVSPVVRTVKKTNLVVNKASRISQNKSPKEDLSKNLKNKEKGKIVEPVRCDDV 218

Query: 290 LKPNSSKLKSVTSSGFSGNIAVHRNNSSKPGE-EGRTSKGIGTKVAGNSVVMSGAKPVDA 349
           L+    ++K V+         +  N SSK    + +    I   V  + V+   +     
Sbjct: 219 LEKTDLEVKKVS--------RISENKSSKEDTLKNKEKAKIDEPVRCDDVLEKTSLDAQK 278

Query: 350 VANLPAIKNENSKVVSRVVSQNKTRRAQTKDAPNVELRKKTLHVSDS---------ETKK 409
           V+ +   +N+NSK       +NK +    +     +  +KTL+V +S          TK 
Sbjct: 279 VSRIS--ENKNSKEERLKNLKNKEKTNIDEPVRPDDAVEKTLYVVESSVEKKKKKMSTKS 338

Query: 410 AKVVESDQNI---------KPALKLCPK--PPSSPYKSSSLADCPSLSPRKENCGETKYK 469
            K+ E+ Q+          K +L L P   PPS     S        + R +     K +
Sbjct: 339 VKISETQQSSEKKIIRSTGKKSLSLLPSLPPPSEVVTGSDPRPIRQTTSRSKTSLPEKKQ 398

Query: 470 RSEANATFSRPNKQGGIKKEEAHNGNKKGRSPRMLPTKGKDSSSLNLNFRNGKVVNLHSE 529
              AN   + P  +  I+ +    G K   +P   PTK +      +NF+ GKV+    E
Sbjct: 399 SGSANLV-TNPKPESKIRPKRI--GLKV--TPPPPPTKQQ------MNFKKGKVLEPKPE 458

Query: 530 SPSARRLKF---------MRGRSLGDNQKSKDGQRTSFKKVVGKGISKDPIPPSEKVVLK 589
             +   +KF         +R   +   +KS   +R    K+ G+G         EKVVL+
Sbjct: 459 DSTTTSIKFKKIVVQEPKLRTSDVNKKKKSLKDKREGVGKINGEG-------KREKVVLR 518

Query: 590 HQAVQGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEKVISLQD 594
           H+ V+ KK  Q LFNNVI ET  KL   RKSKVKALVGAFE VISLQD
Sbjct: 519 HRKVEVKKKLQTLFNNVIEETVNKLEEVRKSKVKALVGAFETVISLQD 538

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144538.11.1e-16546.42flocculation protein FLO11-like isoform X1 [Momordica charantia] >XP_022144539.1... [more]
XP_022144540.14.8e-16446.34flocculation protein FLO11-like isoform X2 [Momordica charantia][more]
KAG6607113.11.8e-16352.34hypothetical protein SDJN03_00455, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023523723.11.8e-16351.22uncharacterized protein LOC111787871 [Cucurbita pepo subsp. pepo] >XP_023523724.... [more]
KAG7036803.12.2e-16150.32hypothetical protein SDJN02_00423, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CSL15.5e-16646.42flocculation protein FLO11-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1CRX02.3e-16446.34flocculation protein FLO11-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1K8S13.1e-16152.44muscle M-line assembly protein unc-89-like isoform X5 OS=Cucurbita maxima OX=366... [more]
A0A6J1GBV94.1e-16150.98muscle M-line assembly protein unc-89-like isoform X2 OS=Cucurbita moschata OX=3... [more]
A0A6J1KF871.1e-15850.96muscle M-line assembly protein unc-89-like isoform X2 OS=Cucurbita maxima OX=366... [more]
Match NameE-valueIdentityDescription
AT5G39380.12.2e-2629.73Plant calmodulin-binding protein-related [more]
AT5G15430.15.3e-2027.52Plant calmodulin-binding protein-related [more]
AT5G61260.14.6e-1628.53Plant calmodulin-binding protein-related [more]
AT5G07820.11.1e-1228.43Plant calmodulin-binding protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 477..590
e-value: 3.7E-25
score: 99.6
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 481..590
e-value: 1.2E-29
score: 103.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 348..362
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..316
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 348..369
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 448..462
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..301
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 208..240
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 398..481
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 12..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 218..233
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 3..597
NoneNo IPR availablePANTHERPTHR33349:SF1EMB|CAB62594.1coord: 3..597

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021002.1Sgr021002.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding