Sgr020944 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr020944
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionHigh-affinity nitrate transporter 2.2
Locationtig00153577: 851546 .. 853359 (-)
RNA-Seq ExpressionSgr020944
SyntenySgr020944
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGTATTGAGGGCTCCCCAGGAAGCTCCATGCATGGAGTCACTGGTAGAGAGCAAACTTTTGCCTTCTCAGTCGCTTCACCGATCGTCCCAACTGATACAACTGCTAAATTTGCTTTACCTGTTGACTCTGAGCATAAGGCCAAGGTTTTCAAGCTCTTCTCTTTGGCCAACCCCCACATGAGAACCTTTCACCTTTCTTGGGTTTCCTTCTTTACTTGCTTCGTCTCCACCTTCGCTGCTGCCCCTCTTGTCCCCATCATCCGCGATAACCTAAACTTGACCAAAAGTGACATTGGAAACGCTGGAGTTGCCTCTGTCTCTGGAAGTATCTTCTCTCGTCTTGTAATGGGTGCTGTTTGTGACCTTCTGGGTCCTCGCTATGGTTGCGCTTTCCTCATAATGCTTTCAGCCCCTACTGTGTTCTGCATGACGTTTGTTTCCTCTGCCGGTGGCTACATAGTCGTTCGATTCATGATCGGATTCTCGCTTGCTACATTCGTGTCATGCCAGTATTGGATGAGTACAATGTTCAACAGTCAGATTATTGGGCTTGTGAATGGAACGGCAGCCGGTTGGGGGAATATGGGCGGAGGAGCAACTCAGCTTCTGATGCCTTTGTTGTATGACATTATAAAGAGAGCTGGTGCCACTCCTTTCACTGCTTGGAGGATTGCTTTCCTCATCCCTGGTTGGCTTCACATTATTATGGGAATTTTAGTTCTAACTCTTGGCCAAGACTTGCCTGATGGGAATCTCAGTAGCCTTCAGAAGAAGGGTAACGTAGCCAAAGATAAATTTTCTAAAGTAAGTTTTTCGTCTTTGAATTGCTTTCTTGTGCGTTCCCTCTCTTTAATTTACTCTACAAAAAAATTGATTCCTTAAGCTAATCATAGACTTCAATTCACGTCTATATTTTAATTTCAGGTGTTATGGTATGCTGTTACTAATTATAGAACTTGGATCTTCGTCCTCCTTTATGGCTACTCAATGGGCGTTGAACTTTCCACCGATAATGTCATTGCTGAATATTTCTACGATAGGTAAATACTCTCAATAAATCAAATATTTTAATGGAAGGCTGCAAAATCTCAAGATCCAAAACTTATTCCCAAGTATTGATTGATTGAATATTATGAAACAGATTTGATCTAAAGCTGCACACGGCGGGAATTATTGCAGCAACCTTTGGCATGGCTAATCTGGTGGCTCGACCATTCGGAGGCTATGCTTCGGACTTTGCAGCTAGATATTTCGGGATGAGAGGAAGACTGTGGACTCTTTGGATCCTCCAAACTTGTGGTGGAGTTTTCTGCATGTGGCTTGGGAAAGCAAACCAGCTTCCAATAGCCATAGTGGCAATGATCTTGTTCTCAATAGGAGCTCAGGCTGCATGTGGAGCCACATTTGGAATCATTCCCTTCATTTCTCGCCGTTCTTTAGGGATTATATCGGGGCTAACGGGCGCCGGAGGGAACTTTGGATCTGGTTTAACGCAATTAGTGTTCTTCTCGACCTCCAAATACTCCACAGACAAAGGCTTGTTCTTGATGGGTATAATGATAGTTTGCTGCACTCTTCCAGTGACGTTGGTCCACTTCCCGCAATGGGGTAGCATGTTCTTACCACCTACTAAACACGTAGAGAAATCTACTGAAGAATTTTACTATGGATCTGAATGGACTGAGGATGAGAAGAAGAAAGGTTTGCACCAGCAGAGCATCAAGTTTGCTGAGAATAGCAGGTCAGAACGTGGCAGGCGTGTTGCCTCAGCTCCAACCCCACCCAATACCACTCCTTCCCATGTTTGA

mRNA sequence

ATGGCTGGTATTGAGGGCTCCCCAGGAAGCTCCATGCATGGAGTCACTGGTAGAGAGCAAACTTTTGCCTTCTCAGTCGCTTCACCGATCGTCCCAACTGATACAACTGCTAAATTTGCTTTACCTGTTGACTCTGAGCATAAGGCCAAGGTTTTCAAGCTCTTCTCTTTGGCCAACCCCCACATGAGAACCTTTCACCTTTCTTGGGTTTCCTTCTTTACTTGCTTCGTCTCCACCTTCGCTGCTGCCCCTCTTGTCCCCATCATCCGCGATAACCTAAACTTGACCAAAAGTGACATTGGAAACGCTGGAGTTGCCTCTGTCTCTGGAAGTATCTTCTCTCGTCTTGTAATGGGTGCTGTTTGTGACCTTCTGGGTCCTCGCTATGGTTGCGCTTTCCTCATAATGCTTTCAGCCCCTACTGTGTTCTGCATGACGTTTGTTTCCTCTGCCGGTGGCTACATAGTCGTTCGATTCATGATCGGATTCTCGCTTGCTACATTCGTGTCATGCCAGTATTGGATGAGTACAATGTTCAACAGTCAGATTATTGGGCTTGTGAATGGAACGGCAGCCGGTTGGGGGAATATGGGCGGAGGAGCAACTCAGCTTCTGATGCCTTTGTTGTATGACATTATAAAGAGAGCTGGTGCCACTCCTTTCACTGCTTGGAGGATTGCTTTCCTCATCCCTGGTTGGCTTCACATTATTATGGGAATTTTAGTTCTAACTCTTGGCCAAGACTTGCCTGATGGGAATCTCAGTAGCCTTCAGAAGAAGGGTAACGTAGCCAAAGATAAATTTTCTAAAGTGTTATGGTATGCTGTTACTAATTATAGAACTTGGATCTTCGTCCTCCTTTATGGCTACTCAATGGGCGTTGAACTTTCCACCGATAATGTCATTGCTGAATATTTCTACGATAGATTTGATCTAAAGCTGCACACGGCGGGAATTATTGCAGCAACCTTTGGCATGGCTAATCTGGTGGCTCGACCATTCGGAGGCTATGCTTCGGACTTTGCAGCTAGATATTTCGGGATGAGAGGAAGACTGTGGACTCTTTGGATCCTCCAAACTTGTGGTGGAGTTTTCTGCATGTGGCTTGGGAAAGCAAACCAGCTTCCAATAGCCATAGTGGCAATGATCTTGTTCTCAATAGGAGCTCAGGCTGCATGTGGAGCCACATTTGGAATCATTCCCTTCATTTCTCGCCGTTCTTTAGGGATTATATCGGGGCTAACGGGCGCCGGAGGGAACTTTGGATCTGGTTTAACGCAATTAGTGTTCTTCTCGACCTCCAAATACTCCACAGACAAAGGCTTGTTCTTGATGGGTATAATGATAGTTTGCTGCACTCTTCCAGTGACGTTGGTCCACTTCCCGCAATGGGGTAGCATGTTCTTACCACCTACTAAACACGTAGAGAAATCTACTGAAGAATTTTACTATGGATCTGAATGGACTGAGGATGAGAAGAAGAAAGGTTTGCACCAGCAGAGCATCAAGTTTGCTGAGAATAGCAGGTCAGAACGTGGCAGGCGTGTTGCCTCAGCTCCAACCCCACCCAATACCACTCCTTCCCATGTTTGA

Coding sequence (CDS)

ATGGCTGGTATTGAGGGCTCCCCAGGAAGCTCCATGCATGGAGTCACTGGTAGAGAGCAAACTTTTGCCTTCTCAGTCGCTTCACCGATCGTCCCAACTGATACAACTGCTAAATTTGCTTTACCTGTTGACTCTGAGCATAAGGCCAAGGTTTTCAAGCTCTTCTCTTTGGCCAACCCCCACATGAGAACCTTTCACCTTTCTTGGGTTTCCTTCTTTACTTGCTTCGTCTCCACCTTCGCTGCTGCCCCTCTTGTCCCCATCATCCGCGATAACCTAAACTTGACCAAAAGTGACATTGGAAACGCTGGAGTTGCCTCTGTCTCTGGAAGTATCTTCTCTCGTCTTGTAATGGGTGCTGTTTGTGACCTTCTGGGTCCTCGCTATGGTTGCGCTTTCCTCATAATGCTTTCAGCCCCTACTGTGTTCTGCATGACGTTTGTTTCCTCTGCCGGTGGCTACATAGTCGTTCGATTCATGATCGGATTCTCGCTTGCTACATTCGTGTCATGCCAGTATTGGATGAGTACAATGTTCAACAGTCAGATTATTGGGCTTGTGAATGGAACGGCAGCCGGTTGGGGGAATATGGGCGGAGGAGCAACTCAGCTTCTGATGCCTTTGTTGTATGACATTATAAAGAGAGCTGGTGCCACTCCTTTCACTGCTTGGAGGATTGCTTTCCTCATCCCTGGTTGGCTTCACATTATTATGGGAATTTTAGTTCTAACTCTTGGCCAAGACTTGCCTGATGGGAATCTCAGTAGCCTTCAGAAGAAGGGTAACGTAGCCAAAGATAAATTTTCTAAAGTGTTATGGTATGCTGTTACTAATTATAGAACTTGGATCTTCGTCCTCCTTTATGGCTACTCAATGGGCGTTGAACTTTCCACCGATAATGTCATTGCTGAATATTTCTACGATAGATTTGATCTAAAGCTGCACACGGCGGGAATTATTGCAGCAACCTTTGGCATGGCTAATCTGGTGGCTCGACCATTCGGAGGCTATGCTTCGGACTTTGCAGCTAGATATTTCGGGATGAGAGGAAGACTGTGGACTCTTTGGATCCTCCAAACTTGTGGTGGAGTTTTCTGCATGTGGCTTGGGAAAGCAAACCAGCTTCCAATAGCCATAGTGGCAATGATCTTGTTCTCAATAGGAGCTCAGGCTGCATGTGGAGCCACATTTGGAATCATTCCCTTCATTTCTCGCCGTTCTTTAGGGATTATATCGGGGCTAACGGGCGCCGGAGGGAACTTTGGATCTGGTTTAACGCAATTAGTGTTCTTCTCGACCTCCAAATACTCCACAGACAAAGGCTTGTTCTTGATGGGTATAATGATAGTTTGCTGCACTCTTCCAGTGACGTTGGTCCACTTCCCGCAATGGGGTAGCATGTTCTTACCACCTACTAAACACGTAGAGAAATCTACTGAAGAATTTTACTATGGATCTGAATGGACTGAGGATGAGAAGAAGAAAGGTTTGCACCAGCAGAGCATCAAGTTTGCTGAGAATAGCAGGTCAGAACGTGGCAGGCGTGTTGCCTCAGCTCCAACCCCACCCAATACCACTCCTTCCCATGTTTGA

Protein sequence

MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGILVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV
Homology
BLAST of Sgr020944 vs. NCBI nr
Match: XP_022964707.1 (high affinity nitrate transporter 2.4-like [Cucurbita moschata])

HSP 1 Score: 1022.7 bits (2643), Expect = 1.1e-294
Identity = 507/530 (95.66%), Postives = 519/530 (97.92%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           M+G+EGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANP
Sbjct: 1   MSGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCM+FVS A GYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
           SQIIGLVNGTAAGWGNMGGGATQL+MPLLYDIIKRAGATPFTAWRIAF IPGWLH+IMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLSSLQKKG+VAKDKFSKVLWYA+TNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA++LP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+KYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYG+EWTEDEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 481 EFYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Sgr020944 vs. NCBI nr
Match: KAG6583556.1 (High-affinity nitrate transporter 2.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583557.1 High-affinity nitrate transporter 2.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.3e-294
Identity = 506/530 (95.47%), Postives = 519/530 (97.92%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           M+G+EGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANP
Sbjct: 1   MSGVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCM+FVS A GYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
           SQIIGLVNGTAAGWGNMGGGATQL+MPLLYDIIKRAGATPFTAWRIAF IPGWLH+IMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLSSLQKKG+VAKDKFSKVLWYA+TNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA++LP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+KYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYG+EWTEDEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 481 EFYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Sgr020944 vs. NCBI nr
Match: XP_023520099.1 (high affinity nitrate transporter 2.4-like [Cucurbita pepo subsp. pepo] >XP_023520141.1 high affinity nitrate transporter 2.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1019.2 bits (2634), Expect = 1.3e-293
Identity = 505/528 (95.64%), Postives = 517/528 (97.92%), Query Frame = 0

Query: 3   GIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHM 62
           G+EGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANPHM
Sbjct: 4   GVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANPHM 63

Query: 63  RTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGAVC 122
           RTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRLVMGAVC
Sbjct: 64  RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC 123

Query: 123 DLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFNSQ 182
           DLLGPRYGCAFLIMLSAPTVFCM+FVS A GYI VRFMIGFSLATFVSCQYWMSTMFNSQ
Sbjct: 124 DLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQ 183

Query: 183 IIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGILV 242
           IIGLVNGTAAGWGNMGGGATQL+MPLLYDIIKRAGATPFTAWRIAF IPGWLH+IMGILV
Sbjct: 184 IIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGILV 243

Query: 243 LTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 302
           LTLGQDLPDGNLSSLQKKG+VAKDKFSKVLWYA+TNYRTWIFVLLYGYSMGVELSTDNVI
Sbjct: 244 LTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDNVI 303

Query: 303 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCG 362
           AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT G
Sbjct: 304 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTFG 363

Query: 363 GVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 422
           GVFCMWLGKA++LP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG
Sbjct: 364 GVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 423

Query: 423 SGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF 482
           SGLTQLVFFST+KY+TDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF
Sbjct: 424 SGLTQLVFFSTTKYTTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF 483

Query: 483 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           YYG+EWTEDEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 484 YYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 531

BLAST of Sgr020944 vs. NCBI nr
Match: XP_022970277.1 (high affinity nitrate transporter 2.4-like [Cucurbita maxima] >XP_022970279.1 high affinity nitrate transporter 2.4-like [Cucurbita maxima] >XP_022970364.1 high affinity nitrate transporter 2.4-like [Cucurbita maxima])

HSP 1 Score: 1016.5 bits (2627), Expect = 8.1e-293
Identity = 503/530 (94.91%), Postives = 517/530 (97.55%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           M+G+EGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANP
Sbjct: 1   MSGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCM+FVS A GYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
           SQIIGLVNGTAAGWGNMGGGATQL+MPLLYDIIKRAGATPFTAWRIAF IPGWLH+IMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLSSLQKKG+V KDKFSKVLWYA+TNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLSSLQKKGDVTKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKL TAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLQTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA++LP AI AMILFSIGAQAACGATFG+IPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGVIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+KYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKH+EKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHLEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYG+EWTEDEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 481 EFYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Sgr020944 vs. NCBI nr
Match: XP_022152832.1 (high affinity nitrate transporter 2.4-like [Momordica charantia])

HSP 1 Score: 1014.6 bits (2622), Expect = 3.1e-292
Identity = 501/527 (95.07%), Postives = 516/527 (97.91%), Query Frame = 0

Query: 4   IEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMR 63
           +EGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVFK+FSLANPHMR
Sbjct: 8   VEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPHMR 67

Query: 64  TFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGAVCD 123
           TFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGV SVSGSIFSRLVMGAVCD
Sbjct: 68  TFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAVCD 127

Query: 124 LLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFNSQI 183
           LLGPRYGCAFLIMLSAPTVFCM+FVSS  GYI VRFMIGFSLATFVSCQYWMSTMFNSQI
Sbjct: 128 LLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQI 187

Query: 184 IGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGILVL 243
           IGLVNGTAAGWGNMGGGATQLLMPLLY+IIK+ GATPFTAWRIAF IPG LH++MGILV+
Sbjct: 188 IGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGILVM 247

Query: 244 TLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIA 303
           TLGQDLPDGNLSSLQKKG VAKDKFS VLWYAVTNYRTWIFVLLYGYSMGVELSTDNV+A
Sbjct: 248 TLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVVA 307

Query: 304 EYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGG 363
           EYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGG
Sbjct: 308 EYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGG 367

Query: 364 VFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGS 423
           VFCMWLGKA QLP+AIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGS
Sbjct: 368 VFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGS 427

Query: 424 GLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFY 483
           GLTQLVFFST+KYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFY
Sbjct: 428 GLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFY 487

Query: 484 YGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           YGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TPSHV
Sbjct: 488 YGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 534

BLAST of Sgr020944 vs. ExPASy Swiss-Prot
Match: Q9FJH8 (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 3.8e-253
Identity = 429/530 (80.94%), Postives = 476/530 (89.81%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           MA   G PGSSMHGVTGREQ++AFSV SP VP+D++AKF+LPVD+EHKAKVFKL S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHL+W+SFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGVASVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF M+FV  AGGYI VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
            QIIGLVNGTAAGWGNMGGG TQLLMP++Y+II+R G+T FTAWR+AF +PGW+HIIMGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGN S+L+KKG V KDKFSKVLWYA+TNYRTW+FVLLYGYSMGVEL+TDN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD AAR FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG FC+WLG+A  LP A+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGN
Sbjct: 361 LGGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTS +ST++GL  MG+MI+ CTLPVTLVHFPQWGSMFLP T+   KSTE
Sbjct: 421 FGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           E+YY  EWTE EK+KG+H+ S+KFA NSRSERGRRVASAP+PP   P HV
Sbjct: 481 EYYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of Sgr020944 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 3.3e-252
Identity = 427/530 (80.57%), Postives = 472/530 (89.06%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           M    G PGSSMHGVTGREQ+FAFSV SPIV TD TAKF LPVD+EHKA VFKLFS A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHLSW+SF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGVASVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF M+FVS A G+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
           SQIIGLVNGTAAGWGNMGGG TQLLMP++Y+II+R G+T FTAWRIAF +PGWLHIIMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVL LGQDLPDGN ++L+K G VAKDKF K+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASDFAA+YFGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN L  A+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGN
Sbjct: 361 AGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQL+FFSTS ++T++GL  MG+MIV CTLPVTLVHFPQWGSMFLPP+    K TE
Sbjct: 421 FGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
             YYGSEW E EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 481 AHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of Sgr020944 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.3e-236
Identity = 402/529 (75.99%), Postives = 453/529 (85.63%), Query Frame = 0

Query: 6   GSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTF 65
           G+PGSS+HGVTGRE  FAFS           +KF LPVDSEHKAK  +L S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGAVCDLL 125
           HLSW+SFF+CFVSTFAAAPLVPIIRDNLNLTK+DIGNAGVASVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFNSQIIG 185
           GPRYGCAFLIML+APTVFCM+ + SA GYI VRF+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGILVLTL 245
           LVNG AAGWGNMGGGATQL+MPL+YD+I++ GATPFTAWR+A+ +PG LH++MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 305
           GQDLPDGNL SLQKKG+V +D FS+VLWYAVTNYRTWIFVLLYGYSMGVEL+TDNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGGVF 365
           FYDRFDL L  AGIIAA+FGMAN+VARP GG  SD  ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 366 CMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 425
           C+ LG+A+ LP ++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 426 TQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYG 485
           TQL+FF++S+YST  GL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 486 SEWTEDEKKKGLHQQSIKFAENSRSERGRR-----VASAPTPPNTTPSH 530
           SEW+E EK KGLH  S+KFAENSRSERGRR      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of Sgr020944 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.3e-236
Identity = 402/529 (75.99%), Postives = 453/529 (85.63%), Query Frame = 0

Query: 6   GSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTF 65
           G+PGSS+HGVTGRE  FAFS           +KF LPVDSEHKAK  +L S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGAVCDLL 125
           HLSW+SFF+CFVSTFAAAPLVPIIRDNLNLTK+DIGNAGVASVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFNSQIIG 185
           GPRYGCAFLIML+APTVFCM+ + SA GYI VRF+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGILVLTL 245
           LVNG AAGWGNMGGGATQL+MPL+YD+I++ GATPFTAWR+A+ +PG LH++MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 305
           GQDLPDGNL SLQKKG+V +D FS+VLWYAVTNYRTWIFVLLYGYSMGVEL+TDNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGGVF 365
           FYDRFDL L  AGIIAA+FGMAN+VARP GG  SD  ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 366 CMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 425
           C+ LG+A+ LP ++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 426 TQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYG 485
           TQL+FF++S+YST  GL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 486 SEWTEDEKKKGLHQQSIKFAENSRSERGRR-----VASAPTPPNTTPSH 530
           SEW+E EK KGLH  S+KFAENSRSERGRR      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of Sgr020944 vs. ExPASy Swiss-Prot
Match: Q9LMZ9 (High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 1.7e-229
Identity = 392/514 (76.26%), Postives = 450/514 (87.55%), Query Frame = 0

Query: 3   GIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHM 62
           G    PGSSMHGVTGREQ++AFSV     PT+T  K+ LPVD+E KA VFKLFS A PHM
Sbjct: 2   GSTDEPGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKPHM 61

Query: 63  RTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGAVC 122
           RTFHLSW+SF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGVASVSGSIFSRLVMGAVC
Sbjct: 62  RTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGAVC 121

Query: 123 DLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFNSQ 182
           DLLGPRYGCAFL+MLSAPTVF M+FVS A G+I VRFMIGF LATFVSCQYWMSTMFNSQ
Sbjct: 122 DLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNSQ 181

Query: 183 IIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGILV 242
           IIGLVNGTAAGWGNMGGG TQLLMP++Y+II+R G+T FTAWRIAF +PGWLHIIMGILV
Sbjct: 182 IIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGILV 241

Query: 243 LTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 302
           LTLGQDLP GN ++++K G VAKDKF K+LWYAVTNYRTWIFVLLYGYSMGVELSTDNVI
Sbjct: 242 LTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 301

Query: 303 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCG 362
           AEYF+DRF LKLHTAGIIAA FGMAN  ARP GG+ASD AA+ FGMRGRLWTLWI+QT G
Sbjct: 302 AEYFFDRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTSG 361

Query: 363 GVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 422
           G+FC+WLG+AN L  A+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNFG
Sbjct: 362 GLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFG 421

Query: 423 SGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF 482
           SGLTQLVFFSTS+++T++GL  MG+MIV CTLPVTL+HFPQWGSMF PP+     +TE +
Sbjct: 422 SGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEHY 481

Query: 483 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRV 517
           Y G E++++E++ G+H +S  FA+ +++E G  V
Sbjct: 482 YVG-EYSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of Sgr020944 vs. ExPASy TrEMBL
Match: A0A6J1HIH1 (high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC111464708 PE=4 SV=1)

HSP 1 Score: 1022.7 bits (2643), Expect = 5.5e-295
Identity = 507/530 (95.66%), Postives = 519/530 (97.92%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           M+G+EGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANP
Sbjct: 1   MSGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCM+FVS A GYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
           SQIIGLVNGTAAGWGNMGGGATQL+MPLLYDIIKRAGATPFTAWRIAF IPGWLH+IMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLSSLQKKG+VAKDKFSKVLWYA+TNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA++LP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+KYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYG+EWTEDEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 481 EFYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Sgr020944 vs. ExPASy TrEMBL
Match: A0A6J1HYN4 (high affinity nitrate transporter 2.4-like OS=Cucurbita maxima OX=3661 GN=LOC111469291 PE=4 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 3.9e-293
Identity = 503/530 (94.91%), Postives = 517/530 (97.55%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           M+G+EGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANP
Sbjct: 1   MSGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCM+FVS A GYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
           SQIIGLVNGTAAGWGNMGGGATQL+MPLLYDIIKRAGATPFTAWRIAF IPGWLH+IMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLSSLQKKG+V KDKFSKVLWYA+TNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLSSLQKKGDVTKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKL TAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLQTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA++LP AI AMILFSIGAQAACGATFG+IPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGVIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+KYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKH+EKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHLEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYG+EWTEDEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 481 EFYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Sgr020944 vs. ExPASy TrEMBL
Match: A0A6J1DHB7 (high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC111020453 PE=4 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.5e-292
Identity = 501/527 (95.07%), Postives = 516/527 (97.91%), Query Frame = 0

Query: 4   IEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMR 63
           +EGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVFK+FSLANPHMR
Sbjct: 8   VEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPHMR 67

Query: 64  TFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGAVCD 123
           TFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGV SVSGSIFSRLVMGAVCD
Sbjct: 68  TFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAVCD 127

Query: 124 LLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFNSQI 183
           LLGPRYGCAFLIMLSAPTVFCM+FVSS  GYI VRFMIGFSLATFVSCQYWMSTMFNSQI
Sbjct: 128 LLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQI 187

Query: 184 IGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGILVL 243
           IGLVNGTAAGWGNMGGGATQLLMPLLY+IIK+ GATPFTAWRIAF IPG LH++MGILV+
Sbjct: 188 IGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGILVM 247

Query: 244 TLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIA 303
           TLGQDLPDGNLSSLQKKG VAKDKFS VLWYAVTNYRTWIFVLLYGYSMGVELSTDNV+A
Sbjct: 248 TLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVVA 307

Query: 304 EYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGG 363
           EYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGG
Sbjct: 308 EYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGG 367

Query: 364 VFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGS 423
           VFCMWLGKA QLP+AIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGS
Sbjct: 368 VFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGS 427

Query: 424 GLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFY 483
           GLTQLVFFST+KYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFY
Sbjct: 428 GLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFY 487

Query: 484 YGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           YGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TPSHV
Sbjct: 488 YGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 534

BLAST of Sgr020944 vs. ExPASy TrEMBL
Match: S4VM42 (High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.1e-290
Identity = 500/530 (94.34%), Postives = 516/530 (97.36%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           M  +EGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVF+++SLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCM+FVS A GYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
           SQIIGLVNGTAAGWGNMGGGATQL+MPLLY++I+RAGATPFTAWRIAF IPG+LH++MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL+SLQKKGNVAKDKFS VLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA +LPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+KYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of Sgr020944 vs. ExPASy TrEMBL
Match: A0A3Q9CZ42 (High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.1e-290
Identity = 499/530 (94.15%), Postives = 517/530 (97.55%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           M  +EGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVF+++SLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCM+FVS+A GYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
           SQIIGLVNGTAAGWGNMGGGATQL+MPLLY++I+RAGATPFTAWRIAF IPG+LH++MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL+SLQKKGNVAKDKFS VLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA +LPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+KYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of Sgr020944 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 875.2 bits (2260), Expect = 2.7e-254
Identity = 429/530 (80.94%), Postives = 476/530 (89.81%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           MA   G PGSSMHGVTGREQ++AFSV SP VP+D++AKF+LPVD+EHKAKVFKL S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHL+W+SFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGVASVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF M+FV  AGGYI VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
            QIIGLVNGTAAGWGNMGGG TQLLMP++Y+II+R G+T FTAWR+AF +PGW+HIIMGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGN S+L+KKG V KDKFSKVLWYA+TNYRTW+FVLLYGYSMGVEL+TDN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD AAR FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG FC+WLG+A  LP A+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGN
Sbjct: 361 LGGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTS +ST++GL  MG+MI+ CTLPVTLVHFPQWGSMFLP T+   KSTE
Sbjct: 421 FGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           E+YY  EWTE EK+KG+H+ S+KFA NSRSERGRRVASAP+PP   P HV
Sbjct: 481 EYYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of Sgr020944 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 872.1 bits (2252), Expect = 2.3e-253
Identity = 427/530 (80.57%), Postives = 472/530 (89.06%), Query Frame = 0

Query: 1   MAGIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANP 60
           M    G PGSSMHGVTGREQ+FAFSV SPIV TD TAKF LPVD+EHKA VFKLFS A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGA 120
           HMRTFHLSW+SF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGVASVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF M+FVS A G+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGI 240
           SQIIGLVNGTAAGWGNMGGG TQLLMP++Y+II+R G+T FTAWRIAF +PGWLHIIMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVL LGQDLPDGN ++L+K G VAKDKF K+LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASDFAA+YFGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN L  A+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGN
Sbjct: 361 AGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQL+FFSTS ++T++GL  MG+MIV CTLPVTLVHFPQWGSMFLPP+    K TE
Sbjct: 421 FGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
             YYGSEW E EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 481 AHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of Sgr020944 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 796.6 bits (2056), Expect = 1.2e-230
Identity = 392/514 (76.26%), Postives = 450/514 (87.55%), Query Frame = 0

Query: 3   GIEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHM 62
           G    PGSSMHGVTGREQ++AFSV     PT+T  K+ LPVD+E KA VFKLFS A PHM
Sbjct: 2   GSTDEPGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKPHM 61

Query: 63  RTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGAVC 122
           RTFHLSW+SF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGVASVSGSIFSRLVMGAVC
Sbjct: 62  RTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGAVC 121

Query: 123 DLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFNSQ 182
           DLLGPRYGCAFL+MLSAPTVF M+FVS A G+I VRFMIGF LATFVSCQYWMSTMFNSQ
Sbjct: 122 DLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNSQ 181

Query: 183 IIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGILV 242
           IIGLVNGTAAGWGNMGGG TQLLMP++Y+II+R G+T FTAWRIAF +PGWLHIIMGILV
Sbjct: 182 IIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGILV 241

Query: 243 LTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 302
           LTLGQDLP GN ++++K G VAKDKF K+LWYAVTNYRTWIFVLLYGYSMGVELSTDNVI
Sbjct: 242 LTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 301

Query: 303 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCG 362
           AEYF+DRF LKLHTAGIIAA FGMAN  ARP GG+ASD AA+ FGMRGRLWTLWI+QT G
Sbjct: 302 AEYFFDRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTSG 361

Query: 363 GVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 422
           G+FC+WLG+AN L  A+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNFG
Sbjct: 362 GLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFG 421

Query: 423 SGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF 482
           SGLTQLVFFSTS+++T++GL  MG+MIV CTLPVTL+HFPQWGSMF PP+     +TE +
Sbjct: 422 SGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEHY 481

Query: 483 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRV 517
           Y G E++++E++ G+H +S  FA+ +++E G  V
Sbjct: 482 YVG-EYSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of Sgr020944 vs. TAIR 10
Match: AT5G60780.1 (nitrate transporter 2.3 )

HSP 1 Score: 791.6 bits (2043), Expect = 4.0e-229
Identity = 390/531 (73.45%), Postives = 453/531 (85.31%), Query Frame = 0

Query: 5   EGSPGSSMHGVTGREQTFAFSV---ASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPH 64
           EGS G+S+HGVT REQ F+FSV   +  +   D TAKFALPVDSEH+AKVF   S A PH
Sbjct: 9   EGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFAKPH 68

Query: 65  MRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVMGAV 124
           MR FHL W+SFFTCF+STFAAAPLVPIIRDNL+LTK+DIGNAGVASVSG+IFSRL MGAV
Sbjct: 69  MRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMGAV 128

Query: 125 CDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTMFNS 184
           CDLLG RYG AF +ML+APTVF M+FV    GY+ VRFMIGF LATFVSCQYW S MFN 
Sbjct: 129 CDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMFNG 188

Query: 185 QIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIMGIL 244
           +IIGLVNG A GWG+MGGG TQLLMP+++ +IK AGATPF AWRIAF +PG+L ++MGIL
Sbjct: 189 KIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMGIL 248

Query: 245 VLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 304
           VL+LGQDLPDGNLS+LQK G V+KDKFSKV W+AV NYRTWI  +LYG SMG+EL+ +NV
Sbjct: 249 VLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTINNV 308

Query: 305 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 364
           I+ YFYDRF+LKL TAGI+AA+FGMAN +ARPFGGYASD AAR FGMRGRLWTLWI QT 
Sbjct: 309 ISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQTV 368

Query: 365 GGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 424
           G +FC+WLG+A+ LPIAI+AM+LFSIG QAACGA FG+ PF+SRRSLG+ISGLTGAGGNF
Sbjct: 369 GALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNF 428

Query: 425 GSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 484
           GSGLTQL+FFS++++ST +GL LMG+M V CTLPV  +HFPQWGSMFL P+   E+S EE
Sbjct: 429 GSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQEE 488

Query: 485 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASA--PTPPNTTPSHV 531
           +YYGSEWTE+EK++GLH+ SIKFAENSRSERGR+VA A  PTP N TPSHV
Sbjct: 489 YYYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 539

BLAST of Sgr020944 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 780.4 bits (2014), Expect = 9.1e-226
Identity = 385/534 (72.10%), Postives = 448/534 (83.90%), Query Frame = 0

Query: 5   EGSPGSSMHGVTGREQTFAFSV------ASPIVPTDTTAKFALPVDSEHKAKVFKLFSLA 64
           +GS G+S+HGVT REQ F+FSV      +  +   D TAKFALPVDSEH+AKVFK  S A
Sbjct: 9   DGSIGTSLHGVTAREQVFSFSVQEDVPSSQAVRTNDPTAKFALPVDSEHRAKVFKPLSFA 68

Query: 65  NPHMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVASVSGSIFSRLVM 124
            PHMR FHL W+SFFTCF+STFAAAPLVP+IRDNL+LTK+DIGNAGVASVSG+IFSRL M
Sbjct: 69  KPHMRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTKTDIGNAGVASVSGAIFSRLAM 128

Query: 125 GAVCDLLGPRYGCAFLIMLSAPTVFCMTFVSSAGGYIVVRFMIGFSLATFVSCQYWMSTM 184
           GAVCDLLG RYG AF +ML+AP VF M+FV+ AG Y+ VRFMIGF LATFVSCQYW S M
Sbjct: 129 GAVCDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAVRFMIGFCLATFVSCQYWTSVM 188

Query: 185 FNSQIIGLVNGTAAGWGNMGGGATQLLMPLLYDIIKRAGATPFTAWRIAFLIPGWLHIIM 244
           F  +IIGLVNG A GWG+MGGG TQLLMP+++ +IK  GATPFTAWR AF IPG L I+M
Sbjct: 189 FTGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTGATPFTAWRFAFFIPGILQIVM 248

Query: 245 GILVLTLGQDLPDGNLSSLQKKGNVAKDKFSKVLWYAVTNYRTWIFVLLYGYSMGVELST 304
           GILVLTLGQDLPDGNLS+LQK G V+KDKFSKV W+AV NYRTWI  +LYG+SMGVEL+ 
Sbjct: 249 GILVLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFMLYGFSMGVELTI 308

Query: 305 DNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWIL 364
           +NVI+ YFYDRF+L LHTAGIIAA+FGMAN  ARPFGGYASD AAR FGMRGRLW LWIL
Sbjct: 309 NNVISGYFYDRFNLTLHTAGIIAASFGMANFFARPFGGYASDVAARLFGMRGRLWILWIL 368

Query: 365 QTCGGVFCMWLGKANQLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAG 424
           QT G +FC+WLG+A+ LPIAI+AM+LFS+G QAACGA FG+ PF+SRRSLG+ISGLTGAG
Sbjct: 369 QTVGALFCIWLGRASSLPIAILAMMLFSMGTQAACGALFGVAPFVSRRSLGLISGLTGAG 428

Query: 425 GNFGSGLTQLVFFSTSKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKS 484
           GNFGSG+TQL+FFS+S++ST +GL LMG+M V C+LPV  +HFPQWGSMFL P++  EKS
Sbjct: 429 GNFGSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVAFIHFPQWGSMFLRPSQDGEKS 488

Query: 485 TEEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRR--VASAPTPPNTTPSHV 531
            EE YYG+EWTE+EK  GLH+ SIKFAENSRSERGR+  +A  PTP   +P+HV
Sbjct: 489 KEEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGRKAMLADIPTPETGSPAHV 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022964707.11.1e-29495.66high affinity nitrate transporter 2.4-like [Cucurbita moschata][more]
KAG6583556.13.3e-29495.47High-affinity nitrate transporter 2.1, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023520099.11.3e-29395.64high affinity nitrate transporter 2.4-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
XP_022970277.18.1e-29394.91high affinity nitrate transporter 2.4-like [Cucurbita maxima] >XP_022970279.1 hi... [more]
XP_022152832.13.1e-29295.07high affinity nitrate transporter 2.4-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9FJH83.8e-25380.94High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 ... [more]
O828113.3e-25280.57High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ... [more]
P0DKG93.3e-23675.99High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKH03.3e-23675.99High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LMZ91.7e-22976.26High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HIH15.5e-29595.66high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HYN43.9e-29394.91high affinity nitrate transporter 2.4-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1DHB71.5e-29295.07high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC... [more]
S4VM423.1e-29094.34High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE... [more]
A0A3Q9CZ423.1e-29094.15High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 ... [more]
Match NameE-valueIdentityDescription
AT5G60770.12.7e-25480.94nitrate transporter 2.4 [more]
AT1G08090.12.3e-25380.57nitrate transporter 2:1 [more]
AT1G08100.11.2e-23076.26nitrate transporter 2.2 [more]
AT5G60780.14.0e-22973.45nitrate transporter 2.3 [more]
AT3G45060.19.1e-22672.10high affinity nitrate transporter 2.6 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 69..425
e-value: 3.9E-21
score: 75.4
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 264..466
e-value: 1.4E-27
score: 98.4
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 58..254
e-value: 3.3E-21
score: 77.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 59..460
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 490..513
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 490..530
NoneNo IPR availablePANTHERPTHR23515:SF16HIGH-AFFINITY NITRATE TRANSPORTER 2.1-LIKEcoord: 1..527
IPR044772Nitrate transporterPANTHERPTHR23515HIGH-AFFINITY NITRATE TRANSPORTER 2.3coord: 1..527
IPR044772Nitrate transporterCDDcd17341MFS_NRT2_likecoord: 65..458
e-value: 1.53802E-138
score: 403.941

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr020944.1Sgr020944.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071249 cellular response to nitrate
biological_process GO:0042128 nitrate assimilation
biological_process GO:0015706 nitrate transport
biological_process GO:0031204 posttranslational protein targeting to membrane, translocation
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0071261 Ssh1 translocon complex
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0008320 protein transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity