Sgr020808 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr020808
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptiontranscription termination factor MTEF18, mitochondrial
Locationtig00153574: 97157 .. 99250 (-)
RNA-Seq ExpressionSgr020808
SyntenySgr020808
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCGAAATCACGATTGCCACAGAGAAGGTGTTGAGCTAGAAATCGTGTTTGCAGCTATTCTTTCTCTTCTATACGGGCGTTTGTTCGATTTTGGCGTTCGGTGTAATGTTCTCTGGTCTCTGTTCATCCCTTGGATCTTCCTCACCGTCTCGTTTCGGCCTCTTGCCATGCTAATTCTTCGAAAGCACGAACGGGTATTGAAATTTTCTAAAATGATTGCCCAACTCAATCACTTGCTTCTCTTTGCGCCTGTTGTCTCTGAAAAAATCATTACTCATAACTCGTCTTTTGTGAGCTTCAAATCTCGATTCTTTTGCAATTGTGGACTTGATTTTCGTCCCAAAGCGTCTCTCATTGAGTCATCTCAATCACCCAGTACAAGTGGCCGAGTATCTAGGATTGCTAGGACAGAGGCTCAGGATGTGCTCTTTGACTATCTGCATTGTACTAGGAGCCTCAGCTTTACAGATGCTGAGCACATAAGCAAGAATTCACCTCATTTTCTTCAACATTTGATCTCGAGACTTGATGGCGAAAAAGATGTAGGTCGGTCTCTCACCAAGTATCTTAGATACAATCCCATCAATGAATTTGAGCCATTCTTTGAAAGCTTAGGACTGCCCCCATCAGAGCTCCCCTTGTTTCTTCCACGACACTTGATGTTTTTGAGTGATGACCATCTTATGCTTGAAAACTTCCACGTGTTATGCAATTATGGAATTCCGCGTAGCAAGCTGGGTAAAATGTACAAGGAAGCAAAAGAGATATTTTCTTATGATTGTGGCCTGTTAGCCTCAAAACTTAGAGCTTATGAAAATTTAGGTCTTGGAAAGGGCACGGTAATTAAGCTTGTTAGTTGCTGCCCGTCGCTTTTGATCGGTTGTATCAGCATGGAGTTCTTCAAGGTTCTTGAGAAGCTAAGTAGGTTAGGTATTGAAAAAGATTGGGTTGGAGGATATATATCTTGTAAAAATACTTATAACTGGAATAGAATGCTTGACACAATGGATTTTCTAGCTAAAGTAGGTTATACAGAGATACAGATGCACAATTTGTTTAAATCAAATCCCTCATTGCTGCTTGAAGATTCTGGGAAGAAAGTGTATGTATTATTGGGTCGTTTAGTCAAGTTGGGTCTTAAGATGGACGAAGCTTATTTGATGTTTATACAAAATCCCGAAATTTTGTCAGGGAAGTATGTTAAAAATATTCTGAAAGCAGTAGATTTTCTCTTTGATATTGGGTTGAGAACAGAGGATATTGCCTGTATAGTATCTCAGCACATTCTGCTACTGGGTTCGTGTACTCTGAAAGGGCCGAAAACCATCTGTAAAGAATTAAAAGTCGGGAAAGAGAGTTTATGTCAGATCATAAGAGACGACCTGTCAAAGCTGTTTAGCTTGGCTTCCAAATCAAAGCTAAAAGCAGCGAACAGGTTTCTTGCCCAGATCCAGCCAAACATTTGGAGAAGACTACATTTCTGCTGAAGTTGGGATACATCGAAAACTCAGACGAGATGGCTAAAGCATTGAAACAGTTTAGGGGTCGTGGAGATCAACTACAGGAGAGATTCGACTGCCTGGTAAAAGCTGGTTTGGACTGTAATGTTGTGACAAACATAATCAGACAAGCTCCCATGGTTCTAAACCAGAGCAAAGATGTGATCCAAGAGAAGATTGATTGTTTAAGAAACTGTTTAGGGTACCCTTTGGATACAGTAGTGGCATTCCCAGCTTATTTATGTTACAACGTGGAGAGAATCAACACAAGATTTTCAATGTATAGGTGGTTGAGGGATAAGGGTGCTGCAAAGCCCAACTTATCATTGAGCAGTGTCTTAGCTTGTTCAGATGCAAGATTTGTAAAATATTTTGTGGATGTTCATCCTGAAGGTCCCACCATGTGGGAAAGATTTAAAAGAGATGGTGTCCATAATCATACTTAATTCTTGGCATTTAATTTTGTTCTATCTTTTTTACCACAATAGCATTTTACTTTGGATGATATTATTCCAAATATGCAGGTCTTTTACGATTCGGCCTGTGTAGTGTATATCTTATTCGTTACTCGTTGCTTCCTGTGCGTTTGTCCATG

mRNA sequence

CTCGAAATCACGATTGCCACAGAGAAGGTGTTGAGCTAGAAATCGTGTTTGCAGCTATTCTTTCTCTTCTATACGGGCGTTTGTTCGATTTTGGCGTTCGGTGTAATGTTCTCTGGTCTCTGTTCATCCCTTGGATCTTCCTCACCGTCTCGTTTCGGCCTCTTGCCATGCTAATTCTTCGAAAGCACGAACGGGTATTGAAATTTTCTAAAATGATTGCCCAACTCAATCACTTGCTTCTCTTTGCGCCTGTTGTCTCTGAAAAAATCATTACTCATAACTCGTCTTTTGTGAGCTTCAAATCTCGATTCTTTTGCAATTGTGGACTTGATTTTCGTCCCAAAGCGTCTCTCATTGAGTCATCTCAATCACCCAGTACAAGTGGCCGAGTATCTAGGATTGCTAGGACAGAGGCTCAGGATGTGCTCTTTGACTATCTGCATTGTACTAGGAGCCTCAGCTTTACAGATGCTGAGCACATAAGCAAGAATTCACCTCATTTTCTTCAACATTTGATCTCGAGACTTGATGGCGAAAAAGATGTAGGTCGGTCTCTCACCAAGTATCTTAGATACAATCCCATCAATGAATTTGAGCCATTCTTTGAAAGCTTAGGACTGCCCCCATCAGAGCTCCCCTTGTTTCTTCCACGACACTTGATGTTTTTGAGTGATGACCATCTTATGCTTGAAAACTTCCACGTGTTATGCAATTATGGAATTCCGCGTAGCAAGCTGGGTAAAATGTACAAGGAAGCAAAAGAGATATTTTCTTATGATTGTGGCCTGTTAGCCTCAAAACTTAGAGCTTATGAAAATTTAGGTCTTGGAAAGGGCACGGTAATTAAGCTTGTTAGTTGCTGCCCGTCGCTTTTGATCGGTTGTATCAGCATGGAGTTCTTCAAGGTTCTTGAGAAGCTAAGTAGGTTAGGTATTGAAAAAGATTGGGTTGGAGGATATATATCTTGTAAAAATACTTATAACTGGAATAGAATGCTTGACACAATGGATTTTCTAGCTAAAGTAGGTTATACAGAGATACAGATGCACAATTTGTTTAAATCAAATCCCTCATTGCTGCTTGAAGATTCTGGGAAGAAAGTGTATGTATTATTGGGTCGTTTAGTCAAGTTGGGTCTTAAGATGGACGAAGCTTATTTGATGTTTATACAAAATCCCGAAATTTTGTCAGGGAAGTATGTTAAAAATATTCTGAAAGCAGTAGATTTTCTCTTTGATATTGGGTTGAGAACAGAGGATATTGCCTGTATAGTATCTCAGCACATTCTGCTACTGGGTTCGTGTACTCTGAAAGGGCCGAAAACCATCTGTAAAGAATTAAAAGTCGGGAAAGAGAGTTTATATCCAGCCAAACATTTGGAGAAGACTACATTTCTGCTGAAGTTGGGATACATCGAAAACTCAGACGAGATGGCTAAAGCATTGAAACAGTTTAGGGGTCGTGGAGATCAACTACAGGAGAGATTCGACTGCCTGGTAAAAGCTGGTTTGGACTGTAATGTTGTGACAAACATAATCAGACAAGCTCCCATGGTTCTAAACCAGAGCAAAGATGTGATCCAAGAGAAGATTGATTGTTTAAGAAACTGTTTAGGGTACCCTTTGGATACAGTAGTGGCATTCCCAGCTTATTTATGTTACAACGTGGAGAGAATCAACACAAGATTTTCAATGTATAGGTGGTTGAGGGATAAGGGTGCTGCAAAGCCCAACTTATCATTGAGCAGTGTCTTAGCTTGTTCAGATGCAAGATTTGTAAAATATTTTGTGGATGTTCATCCTGAAGGTCTTTTACGATTCGGCCTGTGTAGTGTATATCTTATTCGTTACTCGTTGCTTCCTGTGCGTTTGTCCATG

Coding sequence (CDS)

CTCGAAATCACGATTGCCACAGAGAAGGTGTTGAGCTAGAAATCGTGTTTGCAGCTATTCTTTCTCTTCTATACGGGCGTTTGTTCGATTTTGGCGTTCGGTGTAATGTTCTCTGGTCTCTGTTCATCCCTTGGATCTTCCTCACCGTCTCGTTTCGGCCTCTTGCCATGCTAATTCTTCGAAAGCACGAACGGGTATTGAAATTTTCTAAAATGATTGCCCAACTCAATCACTTGCTTCTCTTTGCGCCTGTTGTCTCTGAAAAAATCATTACTCATAACTCGTCTTTTGTGAGCTTCAAATCTCGATTCTTTTGCAATTGTGGACTTGATTTTCGTCCCAAAGCGTCTCTCATTGAGTCATCTCAATCACCCAGTACAAGTGGCCGAGTATCTAGGATTGCTAGGACAGAGGCTCAGGATGTGCTCTTTGACTATCTGCATTGTACTAGGAGCCTCAGCTTTACAGATGCTGAGCACATAAGCAAGAATTCACCTCATTTTCTTCAACATTTGATCTCGAGACTTGATGGCGAAAAAGATGTAGGTCGGTCTCTCACCAAGTATCTTAGATACAATCCCATCAATGAATTTGAGCCATTCTTTGAAAGCTTAGGACTGCCCCCATCAGAGCTCCCCTTGTTTCTTCCACGACACTTGATGTTTTTGAGTGATGACCATCTTATGCTTGAAAACTTCCACGTGTTATGCAATTATGGAATTCCGCGTAGCAAGCTGGGTAAAATGTACAAGGAAGCAAAAGAGATATTTTCTTATGATTGTGGCCTGTTAGCCTCAAAACTTAGAGCTTATGAAAATTTAGGTCTTGGAAAGGGCACGGTAATTAAGCTTGTTAGTTGCTGCCCGTCGCTTTTGATCGGTTGTATCAGCATGGAGTTCTTCAAGGTTCTTGAGAAGCTAAGTAGGTTAGGTATTGAAAAAGATTGGGTTGGAGGATATATATCTTGTAAAAATACTTATAACTGGAATAGAATGCTTGACACAATGGATTTTCTAGCTAAAGTAGGTTATACAGAGATACAGATGCACAATTTGTTTAAATCAAATCCCTCATTGCTGCTTGAAGATTCTGGGAAGAAAGTGTATGTATTATTGGGTCGTTTAGTCAAGTTGGGTCTTAAGATGGACGAAGCTTATTTGATGTTTATACAAAATCCCGAAATTTTGTCAGGGAAGTATGTTAAAAATATTCTGAAAGCAGTAGATTTTCTCTTTGATATTGGGTTGAGAACAGAGGATATTGCCTGTATAGTATCTCAGCACATTCTGCTACTGGGTTCGTGTACTCTGAAAGGGCCGAAAACCATCTGTAAAGAATTAAAAGTCGGGAAAGAGAGTTTATATCCAGCCAAACATTTGGAGAAGACTACATTTCTGCTGAAGTTGGGATACATCGAAAACTCAGACGAGATGGCTAAAGCATTGAAACAGTTTAGGGGTCGTGGAGATCAACTACAGGAGAGATTCGACTGCCTGGTAAAAGCTGGTTTGGACTGTAATGTTGTGACAAACATAATCAGACAAGCTCCCATGGTTCTAAACCAGAGCAAAGATGTGATCCAAGAGAAGATTGATTGTTTAAGAAACTGTTTAGGGTACCCTTTGGATACAGTAGTGGCATTCCCAGCTTATTTATGTTACAACGTGGAGAGAATCAACACAAGATTTTCAATGTATAGGTGGTTGAGGGATAAGGGTGCTGCAAAGCCCAACTTATCATTGAGCAGTGTCTTAGCTTGTTCAGATGCAAGATTTGTAAAATATTTTGTGGATGTTCATCCTGAAGGTCTTTTACGATTCGGCCTGTGTAGTGTATATCTTATTCGTTACTCGTTGCTTCCTGTGCGTTTGTCCATG

Protein sequence

RNHDCHREGVELEIVFAAILSLLYGRLFDFGVRCNVLWSLFIPWIFLTVSFRPLAMLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKIITHNSSFVSFKSRFFCNCGLDFRPKASLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLISRLDGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIGCISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFKSNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDIGLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESLYPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLDCNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSMYRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEGLLRFGLCSVYLIRYSLLPVRLSM
Homology
BLAST of Sgr020808 vs. NCBI nr
Match: XP_022142385.1 (transcription termination factor MTEF18, mitochondrial [Momordica charantia])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.4e-291
Identity = 500/576 (86.81%), Postives = 526/576 (91.32%), Query Frame = 0

Query: 56  MLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKIITHNSSFVSFKSRFFCNCGLDFRPKA 115
           M ILR HERVLKFSKMIAQLNHLLLFAPVVSEK++T+N SFVSFKSRFFCNCG+DF+PKA
Sbjct: 1   MPILRSHERVLKFSKMIAQLNHLLLFAPVVSEKVVTYNPSFVSFKSRFFCNCGIDFQPKA 60

Query: 116 SLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLISRL 175
           SL+ES QSP+TSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQ+LI +L
Sbjct: 61  SLVESVQSPNTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQNLILKL 120

Query: 176 DGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVL 235
           DGEKDV RSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVL
Sbjct: 121 DGEKDVARSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVL 180

Query: 236 CNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIGCI 295
           CNYGIPRSK+GKMYKEAKEIF YD GLLASKLRAYENLGLGKGTVIKLVSCCPSLL G I
Sbjct: 181 CNYGIPRSKMGKMYKEAKEIFDYDYGLLASKLRAYENLGLGKGTVIKLVSCCPSLLXGRI 240

Query: 296 SMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFKSN 355
           SMEFFKVLEKLSRLGI KDW+GGYISC+NTYNWNRML+TMDFLAKVGYTEIQMHNLF+SN
Sbjct: 241 SMEFFKVLEKLSRLGIGKDWIGGYISCRNTYNWNRMLNTMDFLAKVGYTEIQMHNLFESN 300

Query: 356 PSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDIGL 415
           PSLLLEDSGKKVYVL GRLVKLGLKMDEAY MFIQ+PEILSGK+VKNIL+A+DFLFD+GL
Sbjct: 301 PSLLLEDSGKKVYVLFGRLVKLGLKMDEAYSMFIQHPEILSGKFVKNILRAIDFLFDVGL 360

Query: 416 RTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESLY--------------------- 475
            TEDI  IVS HILLLGSCTLKGP+T+CKELKVGKESL                      
Sbjct: 361 GTEDITSIVSHHILLLGSCTLKGPRTVCKELKVGKESLCXIIRDDPSKLFTLASKSKLKS 420

Query: 476 --------PAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLDCN 535
                   P K LEKTTFLLKLGY+ENSDEMAKALK+FRGRGDQLQERFDCLVKAGLDCN
Sbjct: 421 SXQDSCPDPTKRLEKTTFLLKLGYLENSDEMAKALKRFRGRGDQLQERFDCLVKAGLDCN 480

Query: 536 VVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSMYR 595
           VVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDT+VAFPAYLCYN+ERINTRFSMYR
Sbjct: 481 VVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTIVAFPAYLCYNMERINTRFSMYR 540

Query: 596 WLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           WLRDKG+AKPNLSLSSVL CSDARFVKYFVDVHPEG
Sbjct: 541 WLRDKGSAKPNLSLSSVLVCSDARFVKYFVDVHPEG 576

BLAST of Sgr020808 vs. NCBI nr
Match: XP_023000703.1 (transcription termination factor MTEF18, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 944.5 bits (2440), Expect = 4.6e-271
Identity = 473/578 (81.83%), Postives = 503/578 (87.02%), Query Frame = 0

Query: 56  MLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKIIT--HNSSFVSFKSRFFCNCGLDFRP 115
           MLILR H+RVL FSKMI QLNHLL FAPV+SEK IT  HN SFVSFKSR FCN G D  P
Sbjct: 1   MLILRYHKRVLTFSKMITQLNHLLFFAPVLSEKTITQAHNPSFVSFKSRLFCNYGFDIHP 60

Query: 116 KASLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLIS 175
           +ASLIES QSP+TSGRVSR ARTEAQ VLFDYLHCTRSL F DAE+ISKNSPHFLQ LI 
Sbjct: 61  RASLIESPQSPTTSGRVSRNARTEAQKVLFDYLHCTRSLGFADAEYISKNSPHFLQDLIL 120

Query: 176 RLDGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH 235
           +LD EKDV RSLTKYLRYNPINEFEPFFESLGLPPSELP FLPRHLMFLSDDHLMLENFH
Sbjct: 121 KLDSEKDVARSLTKYLRYNPINEFEPFFESLGLPPSELPSFLPRHLMFLSDDHLMLENFH 180

Query: 236 VLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIG 295
           VLCNYGIPRSK+GKMYKEA+E+F+YD GLLASKLRAYE+LGLG GTVIKLVSCCPSLLIG
Sbjct: 181 VLCNYGIPRSKIGKMYKEAREVFAYDYGLLASKLRAYEDLGLGMGTVIKLVSCCPSLLIG 240

Query: 296 CISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFK 355
            I MEFFKVLEKLS+LGIEKDW+GGYIS K+TYNWNR++DT+DFLAKVGYTEIQMH+LFK
Sbjct: 241 QIKMEFFKVLEKLSKLGIEKDWIGGYISYKSTYNWNRLVDTVDFLAKVGYTEIQMHDLFK 300

Query: 356 SNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDI 415
           SNPSLLLEDSGKKVYVL GRLVKLGLKMDEAY ++ QNP ILSG YVKNIL+AVDFLF+I
Sbjct: 301 SNPSLLLEDSGKKVYVLFGRLVKLGLKMDEAYSIYKQNPVILSGNYVKNILRAVDFLFEI 360

Query: 416 GLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESL-------------------- 475
           GL TEDIA IVS  ILLLGSCTLKGPKT+CKELKVGKE L                    
Sbjct: 361 GLGTEDIAGIVSHQILLLGSCTLKGPKTVCKELKVGKEGLCLIIRDDPSKLFTLASKSKL 420

Query: 476 ---------YPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLD 535
                     PAK +EKT FLLKLGY+ENSDE+AKALKQFRGRGDQLQERFDCLV AGLD
Sbjct: 421 KSSEQASCQNPAKQMEKTAFLLKLGYVENSDELAKALKQFRGRGDQLQERFDCLVNAGLD 480

Query: 536 CNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSM 595
           C+VVTNIIR APMVLNQSKDVIQEKIDCLRNCLGYPL T+ AFP YLCYN+ERINTRFSM
Sbjct: 481 CHVVTNIIRHAPMVLNQSKDVIQEKIDCLRNCLGYPLHTIAAFPVYLCYNMERINTRFSM 540

Query: 596 YRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           YRWLRDKGAAKPNLSLS+VLACSDARFVKYFVDVHPEG
Sbjct: 541 YRWLRDKGAAKPNLSLSTVLACSDARFVKYFVDVHPEG 578

BLAST of Sgr020808 vs. NCBI nr
Match: XP_022927279.1 (transcription termination factor MTEF18, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 942.2 bits (2434), Expect = 2.3e-270
Identity = 471/578 (81.49%), Postives = 503/578 (87.02%), Query Frame = 0

Query: 56  MLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKIIT--HNSSFVSFKSRFFCNCGLDFRP 115
           MLILR H+RVLKFSKMI QLNHLL FAPV+SEK IT  HN SF SFKSR FCN G D  P
Sbjct: 1   MLILRYHKRVLKFSKMITQLNHLLFFAPVLSEKTITQAHNPSFFSFKSRLFCNYGFDNHP 60

Query: 116 KASLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLIS 175
           +ASLIES QSP+TSGRVSR ARTEAQ VLFDYLHCTRSL F DAEHISKNSPHFLQ+LI 
Sbjct: 61  RASLIESPQSPTTSGRVSRYARTEAQKVLFDYLHCTRSLGFADAEHISKNSPHFLQNLIM 120

Query: 176 RLDGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH 235
           +LD EKDV RSL KYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH
Sbjct: 121 KLDSEKDVARSLRKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH 180

Query: 236 VLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIG 295
           VLCNYGIPRSK+GKMYKEA+E+F+YD GLLASKLRAYE+LGLG GTVIKLVSCCPSLLIG
Sbjct: 181 VLCNYGIPRSKMGKMYKEAREVFAYDYGLLASKLRAYEDLGLGMGTVIKLVSCCPSLLIG 240

Query: 296 CISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFK 355
            I MEF KVLEKLS+LGIE+DW+GGYIS K+TYNWNR++DT+DFLAKVGYTEIQMH+LFK
Sbjct: 241 QIKMEFIKVLEKLSKLGIEEDWIGGYISHKSTYNWNRLVDTVDFLAKVGYTEIQMHDLFK 300

Query: 356 SNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDI 415
           SNPSLLLEDSGKKVYVL GRLVKLGLKM+EAY ++ QNP ILSGKYVKNIL+AVDFLFDI
Sbjct: 301 SNPSLLLEDSGKKVYVLFGRLVKLGLKMNEAYSIYKQNPVILSGKYVKNILRAVDFLFDI 360

Query: 416 GLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESL-------------------- 475
           GL TEDIA IVS  ILLLGSCTLKGPKT+CKELKVGKE L                    
Sbjct: 361 GLGTEDIAGIVSHQILLLGSCTLKGPKTVCKELKVGKEGLCLIIRDDPSKLFALASKSKL 420

Query: 476 ---------YPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLD 535
                     PAK +EKTTFLLKLGY+ENSDE+AKA KQFRGRGDQLQERFDCLV AGLD
Sbjct: 421 KSSEQASCQNPAKQMEKTTFLLKLGYVENSDELAKASKQFRGRGDQLQERFDCLVNAGLD 480

Query: 536 CNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSM 595
           C+VVTNI+R APMVLNQSKDVIQEKIDCLRNCLGYPL T+ AFP YLCY +ERINTRFSM
Sbjct: 481 CHVVTNIVRHAPMVLNQSKDVIQEKIDCLRNCLGYPLHTIAAFPVYLCYKMERINTRFSM 540

Query: 596 YRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           YRWLRDKGAAKPNLSLS+VLACSDARFVKYFVDVHPEG
Sbjct: 541 YRWLRDKGAAKPNLSLSTVLACSDARFVKYFVDVHPEG 578

BLAST of Sgr020808 vs. NCBI nr
Match: XP_023519412.1 (transcription termination factor MTEF18, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941.0 bits (2431), Expect = 5.1e-270
Identity = 471/578 (81.49%), Postives = 502/578 (86.85%), Query Frame = 0

Query: 56  MLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKIIT--HNSSFVSFKSRFFCNCGLDFRP 115
           MLILR H+RVLKFSKMI QLNHLL FAPV+SEK IT  HN SFVSFKSR FCN G D  P
Sbjct: 1   MLILRYHKRVLKFSKMITQLNHLLFFAPVLSEKTITQAHNPSFVSFKSRLFCNYGFDIHP 60

Query: 116 KASLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLIS 175
           +ASLIES QSP+T GRVSR ARTEAQ VLFDYLHCTRSL F DAEHISKNSPHFLQ+LI 
Sbjct: 61  RASLIESPQSPTTGGRVSRYARTEAQKVLFDYLHCTRSLGFADAEHISKNSPHFLQNLIM 120

Query: 176 RLDGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH 235
           +LD EKDV RSL KYLRYNPINEFEPFFESLGLPPSELPLFLPR LMFLSDDHLMLENFH
Sbjct: 121 KLDSEKDVARSLRKYLRYNPINEFEPFFESLGLPPSELPLFLPRRLMFLSDDHLMLENFH 180

Query: 236 VLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIG 295
           VLCNYGIPRSK+GKMYKEA+EIF+YD GLLASKLRAYE+LGLG GT+IKLVSCCPSLLIG
Sbjct: 181 VLCNYGIPRSKMGKMYKEAREIFAYDYGLLASKLRAYEDLGLGMGTLIKLVSCCPSLLIG 240

Query: 296 CISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFK 355
            I MEF KVLEKLS+LGIE+DW+GGYIS K+TYNWNR++DT+DFLAKVGYTEIQMH+LFK
Sbjct: 241 QIKMEFIKVLEKLSKLGIEEDWIGGYISHKSTYNWNRLVDTLDFLAKVGYTEIQMHDLFK 300

Query: 356 SNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDI 415
           SNPSLLLEDSGKKVYVL  RLVKLGLKMDEAY ++ QNP ILSGKYVKNIL+AVDFLFDI
Sbjct: 301 SNPSLLLEDSGKKVYVLFVRLVKLGLKMDEAYSIYKQNPVILSGKYVKNILRAVDFLFDI 360

Query: 416 GLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESL-------------------- 475
           GL TEDIA IVS  ILLLGSCTLKGPKT+CKELKVGKE L                    
Sbjct: 361 GLGTEDIAGIVSHQILLLGSCTLKGPKTVCKELKVGKEGLCLIIRDDPSKLFTLASKSKL 420

Query: 476 ---------YPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLD 535
                     PAK +EKTTFLLKLGY+ENSDE+AKA KQFRGRGDQLQERFDCLV AGLD
Sbjct: 421 KSSEQASCQNPAKEMEKTTFLLKLGYVENSDELAKASKQFRGRGDQLQERFDCLVNAGLD 480

Query: 536 CNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSM 595
           C+VVTNI+R APMVLNQSKDVIQEKIDCLRNCLGYPL T+ AFP YLCYN+ERINTRFSM
Sbjct: 481 CHVVTNIVRHAPMVLNQSKDVIQEKIDCLRNCLGYPLHTIAAFPVYLCYNMERINTRFSM 540

Query: 596 YRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           YRWLRDKGAAKPNLSLS+VLACSDARFVKYFVDVHPEG
Sbjct: 541 YRWLRDKGAAKPNLSLSTVLACSDARFVKYFVDVHPEG 578

BLAST of Sgr020808 vs. NCBI nr
Match: XP_038893610.1 (transcription termination factor MTEF18, mitochondrial [Benincasa hispida])

HSP 1 Score: 940.3 bits (2429), Expect = 8.7e-270
Identity = 464/578 (80.28%), Postives = 506/578 (87.54%), Query Frame = 0

Query: 56  MLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKII--THNSSFVSFKSRFFCNCGLDFRP 115
           M ILRKHERVLKFSKMI +LNHLL F PVVSEKI   TH  SF+SF  R FCNCG DF P
Sbjct: 1   MPILRKHERVLKFSKMITKLNHLLFFGPVVSEKITVQTHTLSFISFNPRLFCNCGFDFHP 60

Query: 116 KASLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLIS 175
           +ASLIES+QSP+ SGRVSRIARTEAQ VLFDYLH TRSLSFTDAEHIS+NSPHFLQ+L+ 
Sbjct: 61  RASLIESAQSPNASGRVSRIARTEAQHVLFDYLHHTRSLSFTDAEHISQNSPHFLQNLLM 120

Query: 176 RLDGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH 235
           +L+ EKDV R L KYLRYNPINEFEPF ESLGLPPSELPL LPRHL++LSDDHLMLEN+H
Sbjct: 121 KLNSEKDVARCLAKYLRYNPINEFEPFLESLGLPPSELPLLLPRHLIYLSDDHLMLENYH 180

Query: 236 VLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIG 295
            LC YGIP+ K+GKMYKEA+E+FSYD G+LASKLRAYENLGLGKGTVIKLV+CCPSLLIG
Sbjct: 181 ALCKYGIPQGKMGKMYKEAREVFSYDYGVLASKLRAYENLGLGKGTVIKLVTCCPSLLIG 240

Query: 296 CISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFK 355
            ISME FKVLEKL+RLGIEKDW+GG+ISCK+TY WNRM+DTMDFL KVGYTEIQMHNLFK
Sbjct: 241 QISMELFKVLEKLNRLGIEKDWIGGHISCKSTYYWNRMVDTMDFLVKVGYTEIQMHNLFK 300

Query: 356 SNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDI 415
           SNP LLLEDSGKK+YVL GRLVKLGLKMDE+Y MF QNPEILSGKY+KNILKAVDFLFDI
Sbjct: 301 SNPPLLLEDSGKKMYVLFGRLVKLGLKMDESYSMFKQNPEILSGKYIKNILKAVDFLFDI 360

Query: 416 GLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESLY------------------- 475
           GL TEDIACIV+ +ILLLGS TLKGPKT+CKELKVGKE L                    
Sbjct: 361 GLGTEDIACIVTHNILLLGSSTLKGPKTVCKELKVGKEDLCMIIRDDPSKLFTLASKSKL 420

Query: 476 ----------PAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLD 535
                     PAK+LEKT FLLKLGY+ENS+EMAKALKQFRGRGDQLQERFDCLVKAGLD
Sbjct: 421 KTSEQVSSQDPAKYLEKTAFLLKLGYLENSNEMAKALKQFRGRGDQLQERFDCLVKAGLD 480

Query: 536 CNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSM 595
           CNVVT+IIR+APMVLNQSKDVI+EKIDCLRNCLGYPL+T+V FPAYLCYN+E+INTRFSM
Sbjct: 481 CNVVTSIIRRAPMVLNQSKDVIEEKIDCLRNCLGYPLNTIVTFPAYLCYNMEKINTRFSM 540

Query: 596 YRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           YRWLRDKGAAKPNLSLS+VLACSDA+FVKYFVDVHPEG
Sbjct: 541 YRWLRDKGAAKPNLSLSTVLACSDAKFVKYFVDVHPEG 578

BLAST of Sgr020808 vs. ExPASy Swiss-Prot
Match: Q9M219 (Transcription termination factor MTEF18, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF18 PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 3.7e-61
Identity = 159/510 (31.18%), Postives = 261/510 (51.18%), Query Frame = 0

Query: 133 IARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLISRLD-GEKDVGRSLTKYLRY 192
           +A  +AQ  + DYLH TRSLS+T AE I+ N+   +++LI +LD       +SL K+L Y
Sbjct: 32  LAIGKAQQAITDYLHTTRSLSYTHAEQIASNASVSIRNLILKLDFSVPTFSKSLRKHLSY 91

Query: 193 NPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVLCNYGIPRSKLGKMYKE 252
           +PINEFE FFES+G+  SE+  FLP    F S+D  +L+    L  +G P +KLGK+YKE
Sbjct: 92  HPINEFEFFFESIGIDYSEVSEFLPEKKFFFSEDRTVLDAAFALSGFGFPWNKLGKLYKE 151

Query: 253 AKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIGC--ISMEFFKVLEKLSRL 312
            + +F    G + S+L  ++++G     VI      P  L G   +  E   +  KL RL
Sbjct: 152 ERLVFVQRPGEIESRLLKFKDIGFSTVAVIGTCLAIPRTLCGGGELGSEIRCLFVKLKRL 211

Query: 313 GIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFKSNPSLLLEDSGKKVYV 372
             E D    ++  +N  +W  +   +     +G    +M  L   N SL LE S + +  
Sbjct: 212 FDEFD--SHHLFEENVDSWLAVSRKIRIFYDLGCENEEMWELMCRNKSLFLEYSEEALMN 271

Query: 373 LLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDIGLRTEDIACIVSQH-- 432
             G   + G+  ++A L+ ++NP I++    K ++     L   GLR +++  +  ++  
Sbjct: 272 KAGYFCRFGVSKEDAALLILRNPAIMNFDLEKPVISVTGMLKHFGLRQDEVDAVAQKYPY 331

Query: 433 -------------------------IL------LLGSCTLKGP-KTICKELKVGKESLYP 492
                                    IL      LL S TL  P + + +E + G E L  
Sbjct: 332 VFGRNQLKNLPYVLRAIDLHERIFDILKNGNHHLLASYTLMDPDEDLEREYQEGLEELQN 391

Query: 493 AK----HLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLDCNVVTNI 552
           ++    +++K  FL ++G+ EN   M K L+   G   +L +RF  L+ +G+  + +  +
Sbjct: 392 SRTKRHNIQKLDFLHEIGFGENGITM-KVLQHVHGTAVELHDRFQILLNSGIIFSKICML 451

Query: 553 IRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVE-RINTRFSMYRWLRD 601
           IR AP +LNQ    IQ+K+  L   +G  LD +  FPAYLC+++E RI+ RF  ++WL +
Sbjct: 452 IRSAPKILNQKPHSIQDKLRFLCGEMGDSLDYLEVFPAYLCFDLENRISPRFRFHKWLVE 511

BLAST of Sgr020808 vs. ExPASy Swiss-Prot
Match: Q9C6A1 (Transcription termination factor MTERF15, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF15 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.1e-12
Identity = 87/397 (21.91%), Postives = 164/397 (41.31%), Query Frame = 0

Query: 233 HVLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLL- 292
           ++L  YG P S L         + + D       L    +L + + +++ L+S CP++L 
Sbjct: 46  NLLQRYGFPPSSLQHFLSRNNHLLNSDLVETEISLGILLSLKIPQKSLVSLISDCPNVLR 105

Query: 293 ----------------IGCISMEFFK-VLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDT 352
                            G +S    K VLE  SR+GI  D               +  + 
Sbjct: 106 SEFLRKWRVPLSNCGKHGVVSSSAIKSVLEHSSRIGIGPD---------------KFNEC 165

Query: 353 MDFLAKVGYTEIQMHNLFKSNPSLLLEDS---GKKVYVLLGRLVKLGLKMDEAYLMFIQN 412
           +  L  +G+ +  +  +  S P +LL +     +K+  L+G    +G+  D     F   
Sbjct: 166 VRVLKSLGFCDSTVSRILSSFPGVLLVNEIEIRRKIEFLVG----IGIARDNIERFFHVF 225

Query: 413 PEILSGKYVKNILKAVDFLFDIGLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKE 472
           PE+L       +   +D    +G   +D+   +++   +LG    + P+  C EL     
Sbjct: 226 PEVLGIGTETRLKPLLDEFMKMGFSKDDVKKEIAREPRVLGLELGELPR--CLEL----- 285

Query: 473 SLYPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLDCNVVTNI 532
                 +  K   ++++  I            FR  G +++ R DCL K GL       +
Sbjct: 286 -----INTLKCREVIRVSIISEG--------AFRA-GFEVKLRVDCLCKYGLIRRDAFKV 345

Query: 533 IRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVER-INTRFSMYRWLRD 592
           + + P V+    + I++KI+ L N +G+ ++ +   P YL  N+++ I  R+++  +L+ 
Sbjct: 346 VWKEPRVILYEIEDIEKKIEFLTNRMGFHINCLADVPEYLGVNLQKQIVPRYNVIDYLKL 402

Query: 593 KGAAKPNLSLSSVLACSDARFVKYFVDVHPEGLLRFG 608
           KG    ++ L  ++  S  RF   +V  +PE    FG
Sbjct: 406 KGGLGCDIGLKGLIKPSMKRFYNLYVMPYPECERIFG 402

BLAST of Sgr020808 vs. ExPASy Swiss-Prot
Match: Q9FK23 (Transcription termination factor MTERF8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF8 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.0e-07
Identity = 51/218 (23.39%), Postives = 97/218 (44.50%), Query Frame = 0

Query: 378 GLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDIGLRTEDIACIVSQHILLLGSCTLK 437
           GL  ++ + +    P ++S    + +   ++FL + G  +  +   +S+  L+L      
Sbjct: 298 GLTSEQVFKIVHVFPNVISTSKERKLRPRIEFLKECGFDSPGMFKFLSKAPLIL------ 357

Query: 438 GPKTICKELKVGKESLYPAKHLEKTTFLLKLGYIENSDEMAKALKQF-RGRGDQLQERFD 497
                         +L       K  FL+K+GY   + E+A A+    R   D +Q    
Sbjct: 358 --------------ALSENNLSHKLGFLVKIGYKHRTKELAFAMGAVTRTSSDNMQRVIG 417

Query: 498 CLVKAGLDCNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVE 557
             +  GL    +  +  + P VL  +   ++EK++ L   +G  ++ ++AFPA+L Y ++
Sbjct: 418 LYLSYGLSFEDILAMSTKHPQVLQYNYTSLEEKLEYLIEYMGREVEELLAFPAFLGYKLD 477

Query: 558 -RINTRFSMYRWLRDKGAAKPNLSLSSVLACSDARFVK 594
            RI  R+     L+ +G    N+SL+ +L  S  RF K
Sbjct: 478 SRIKHRYE--EKLKSRG---ENMSLNKLLTVSAERFSK 490

BLAST of Sgr020808 vs. ExPASy TrEMBL
Match: A0A6J1CKT4 (transcription termination factor MTEF18, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111012532 PE=3 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 6.7e-292
Identity = 500/576 (86.81%), Postives = 526/576 (91.32%), Query Frame = 0

Query: 56  MLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKIITHNSSFVSFKSRFFCNCGLDFRPKA 115
           M ILR HERVLKFSKMIAQLNHLLLFAPVVSEK++T+N SFVSFKSRFFCNCG+DF+PKA
Sbjct: 1   MPILRSHERVLKFSKMIAQLNHLLLFAPVVSEKVVTYNPSFVSFKSRFFCNCGIDFQPKA 60

Query: 116 SLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLISRL 175
           SL+ES QSP+TSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQ+LI +L
Sbjct: 61  SLVESVQSPNTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQNLILKL 120

Query: 176 DGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVL 235
           DGEKDV RSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVL
Sbjct: 121 DGEKDVARSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVL 180

Query: 236 CNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIGCI 295
           CNYGIPRSK+GKMYKEAKEIF YD GLLASKLRAYENLGLGKGTVIKLVSCCPSLL G I
Sbjct: 181 CNYGIPRSKMGKMYKEAKEIFDYDYGLLASKLRAYENLGLGKGTVIKLVSCCPSLLXGRI 240

Query: 296 SMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFKSN 355
           SMEFFKVLEKLSRLGI KDW+GGYISC+NTYNWNRML+TMDFLAKVGYTEIQMHNLF+SN
Sbjct: 241 SMEFFKVLEKLSRLGIGKDWIGGYISCRNTYNWNRMLNTMDFLAKVGYTEIQMHNLFESN 300

Query: 356 PSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDIGL 415
           PSLLLEDSGKKVYVL GRLVKLGLKMDEAY MFIQ+PEILSGK+VKNIL+A+DFLFD+GL
Sbjct: 301 PSLLLEDSGKKVYVLFGRLVKLGLKMDEAYSMFIQHPEILSGKFVKNILRAIDFLFDVGL 360

Query: 416 RTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESLY--------------------- 475
            TEDI  IVS HILLLGSCTLKGP+T+CKELKVGKESL                      
Sbjct: 361 GTEDITSIVSHHILLLGSCTLKGPRTVCKELKVGKESLCXIIRDDPSKLFTLASKSKLKS 420

Query: 476 --------PAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLDCN 535
                   P K LEKTTFLLKLGY+ENSDEMAKALK+FRGRGDQLQERFDCLVKAGLDCN
Sbjct: 421 SXQDSCPDPTKRLEKTTFLLKLGYLENSDEMAKALKRFRGRGDQLQERFDCLVKAGLDCN 480

Query: 536 VVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSMYR 595
           VVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDT+VAFPAYLCYN+ERINTRFSMYR
Sbjct: 481 VVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTIVAFPAYLCYNMERINTRFSMYR 540

Query: 596 WLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           WLRDKG+AKPNLSLSSVL CSDARFVKYFVDVHPEG
Sbjct: 541 WLRDKGSAKPNLSLSSVLVCSDARFVKYFVDVHPEG 576

BLAST of Sgr020808 vs. ExPASy TrEMBL
Match: A0A6J1KND2 (transcription termination factor MTEF18, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111495068 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.2e-271
Identity = 473/578 (81.83%), Postives = 503/578 (87.02%), Query Frame = 0

Query: 56  MLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKIIT--HNSSFVSFKSRFFCNCGLDFRP 115
           MLILR H+RVL FSKMI QLNHLL FAPV+SEK IT  HN SFVSFKSR FCN G D  P
Sbjct: 1   MLILRYHKRVLTFSKMITQLNHLLFFAPVLSEKTITQAHNPSFVSFKSRLFCNYGFDIHP 60

Query: 116 KASLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLIS 175
           +ASLIES QSP+TSGRVSR ARTEAQ VLFDYLHCTRSL F DAE+ISKNSPHFLQ LI 
Sbjct: 61  RASLIESPQSPTTSGRVSRNARTEAQKVLFDYLHCTRSLGFADAEYISKNSPHFLQDLIL 120

Query: 176 RLDGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH 235
           +LD EKDV RSLTKYLRYNPINEFEPFFESLGLPPSELP FLPRHLMFLSDDHLMLENFH
Sbjct: 121 KLDSEKDVARSLTKYLRYNPINEFEPFFESLGLPPSELPSFLPRHLMFLSDDHLMLENFH 180

Query: 236 VLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIG 295
           VLCNYGIPRSK+GKMYKEA+E+F+YD GLLASKLRAYE+LGLG GTVIKLVSCCPSLLIG
Sbjct: 181 VLCNYGIPRSKIGKMYKEAREVFAYDYGLLASKLRAYEDLGLGMGTVIKLVSCCPSLLIG 240

Query: 296 CISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFK 355
            I MEFFKVLEKLS+LGIEKDW+GGYIS K+TYNWNR++DT+DFLAKVGYTEIQMH+LFK
Sbjct: 241 QIKMEFFKVLEKLSKLGIEKDWIGGYISYKSTYNWNRLVDTVDFLAKVGYTEIQMHDLFK 300

Query: 356 SNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDI 415
           SNPSLLLEDSGKKVYVL GRLVKLGLKMDEAY ++ QNP ILSG YVKNIL+AVDFLF+I
Sbjct: 301 SNPSLLLEDSGKKVYVLFGRLVKLGLKMDEAYSIYKQNPVILSGNYVKNILRAVDFLFEI 360

Query: 416 GLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESL-------------------- 475
           GL TEDIA IVS  ILLLGSCTLKGPKT+CKELKVGKE L                    
Sbjct: 361 GLGTEDIAGIVSHQILLLGSCTLKGPKTVCKELKVGKEGLCLIIRDDPSKLFTLASKSKL 420

Query: 476 ---------YPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLD 535
                     PAK +EKT FLLKLGY+ENSDE+AKALKQFRGRGDQLQERFDCLV AGLD
Sbjct: 421 KSSEQASCQNPAKQMEKTAFLLKLGYVENSDELAKALKQFRGRGDQLQERFDCLVNAGLD 480

Query: 536 CNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSM 595
           C+VVTNIIR APMVLNQSKDVIQEKIDCLRNCLGYPL T+ AFP YLCYN+ERINTRFSM
Sbjct: 481 CHVVTNIIRHAPMVLNQSKDVIQEKIDCLRNCLGYPLHTIAAFPVYLCYNMERINTRFSM 540

Query: 596 YRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           YRWLRDKGAAKPNLSLS+VLACSDARFVKYFVDVHPEG
Sbjct: 541 YRWLRDKGAAKPNLSLSTVLACSDARFVKYFVDVHPEG 578

BLAST of Sgr020808 vs. ExPASy TrEMBL
Match: A0A6J1EHL0 (transcription termination factor MTEF18, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111434165 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 1.1e-270
Identity = 471/578 (81.49%), Postives = 503/578 (87.02%), Query Frame = 0

Query: 56  MLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKIIT--HNSSFVSFKSRFFCNCGLDFRP 115
           MLILR H+RVLKFSKMI QLNHLL FAPV+SEK IT  HN SF SFKSR FCN G D  P
Sbjct: 1   MLILRYHKRVLKFSKMITQLNHLLFFAPVLSEKTITQAHNPSFFSFKSRLFCNYGFDNHP 60

Query: 116 KASLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLIS 175
           +ASLIES QSP+TSGRVSR ARTEAQ VLFDYLHCTRSL F DAEHISKNSPHFLQ+LI 
Sbjct: 61  RASLIESPQSPTTSGRVSRYARTEAQKVLFDYLHCTRSLGFADAEHISKNSPHFLQNLIM 120

Query: 176 RLDGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH 235
           +LD EKDV RSL KYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH
Sbjct: 121 KLDSEKDVARSLRKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFH 180

Query: 236 VLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIG 295
           VLCNYGIPRSK+GKMYKEA+E+F+YD GLLASKLRAYE+LGLG GTVIKLVSCCPSLLIG
Sbjct: 181 VLCNYGIPRSKMGKMYKEAREVFAYDYGLLASKLRAYEDLGLGMGTVIKLVSCCPSLLIG 240

Query: 296 CISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFK 355
            I MEF KVLEKLS+LGIE+DW+GGYIS K+TYNWNR++DT+DFLAKVGYTEIQMH+LFK
Sbjct: 241 QIKMEFIKVLEKLSKLGIEEDWIGGYISHKSTYNWNRLVDTVDFLAKVGYTEIQMHDLFK 300

Query: 356 SNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDI 415
           SNPSLLLEDSGKKVYVL GRLVKLGLKM+EAY ++ QNP ILSGKYVKNIL+AVDFLFDI
Sbjct: 301 SNPSLLLEDSGKKVYVLFGRLVKLGLKMNEAYSIYKQNPVILSGKYVKNILRAVDFLFDI 360

Query: 416 GLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESL-------------------- 475
           GL TEDIA IVS  ILLLGSCTLKGPKT+CKELKVGKE L                    
Sbjct: 361 GLGTEDIAGIVSHQILLLGSCTLKGPKTVCKELKVGKEGLCLIIRDDPSKLFALASKSKL 420

Query: 476 ---------YPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLD 535
                     PAK +EKTTFLLKLGY+ENSDE+AKA KQFRGRGDQLQERFDCLV AGLD
Sbjct: 421 KSSEQASCQNPAKQMEKTTFLLKLGYVENSDELAKASKQFRGRGDQLQERFDCLVNAGLD 480

Query: 536 CNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSM 595
           C+VVTNI+R APMVLNQSKDVIQEKIDCLRNCLGYPL T+ AFP YLCY +ERINTRFSM
Sbjct: 481 CHVVTNIVRHAPMVLNQSKDVIQEKIDCLRNCLGYPLHTIAAFPVYLCYKMERINTRFSM 540

Query: 596 YRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           YRWLRDKGAAKPNLSLS+VLACSDARFVKYFVDVHPEG
Sbjct: 541 YRWLRDKGAAKPNLSLSTVLACSDARFVKYFVDVHPEG 578

BLAST of Sgr020808 vs. ExPASy TrEMBL
Match: A0A2N9ISM1 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55025 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 2.2e-218
Identity = 381/562 (67.79%), Postives = 447/562 (79.54%), Query Frame = 0

Query: 71  MIAQLNHLLLFAPVVSEKIIT-HNSSFVSFKSRFFCNCGLDFRPKASLIESSQSPSTSGR 130
           MIA+LNH +LF+PV  +K+IT  N SF+ FK R F    L   PK  + ES+ S + + R
Sbjct: 1   MIAKLNHPVLFSPVGYDKVITKQNPSFIFFKVRCFRTSTLTNHPKVIVTESALSSNFTPR 60

Query: 131 VSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLISRLDGEKDVGRSLTKYL 190
            SR+A+ EAQ VLFDYLHCTRS SF DAEHISKNSPHFLQ L+S++D +K V  SLTK+L
Sbjct: 61  FSRLAKNEAQHVLFDYLHCTRSFSFMDAEHISKNSPHFLQILLSKVDTDKQVAHSLTKFL 120

Query: 191 RYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVLCNYGIPRSKLGKMY 250
           RYNPINEFEPFFESLGL PSEL + LPRHLMFLSDDH+ML+NFHVLCNYGIPRSK+GKMY
Sbjct: 121 RYNPINEFEPFFESLGLSPSELSMLLPRHLMFLSDDHVMLDNFHVLCNYGIPRSKMGKMY 180

Query: 251 KEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIGCISMEFFKVLEKLSRL 310
           KEAKEIF YD G+LA KL+AYE LGL K TVIKL+SCCPSLLIG ++ EF KVLE+L  L
Sbjct: 181 KEAKEIFGYDYGILALKLQAYEKLGLSKPTVIKLISCCPSLLIGGVNCEFVKVLERLKEL 240

Query: 311 GIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFKSNPSLLLEDSGKKVYV 370
             E DW+GGY+S K+TYNWNRMLDT+ FL  VGY+E QM  LF +NP+LL E SGK VYV
Sbjct: 241 RFENDWIGGYLSGKSTYNWNRMLDTVIFLDTVGYSEEQMRYLFNTNPALLFEGSGKNVYV 300

Query: 371 LLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDIGLRTEDIACIVSQHIL 430
           L GRL+K GLKM+E Y +FIQNP ILS K +KN+L+AVDFL +I + TE IA I+S HI 
Sbjct: 301 LFGRLLKSGLKMNEIYSLFIQNPNILSVKCIKNLLRAVDFLLEIEMGTEYIASIISNHIQ 360

Query: 431 LLGSCTLKGPKTICKELKVGKESLY-----------------------------PAKHLE 490
           LLGSCTLKGPKT+CKELKVG++ L                              P+KH E
Sbjct: 361 LLGSCTLKGPKTVCKELKVGRDGLCQIIKEDPLKLFNLASKSKIKSSEQASSQDPSKHQE 420

Query: 491 KTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLDCNVVTNIIRQAPMVLN 550
           KTTFL++LGY+ENSDEM KALKQFRGRGDQLQERFDCLV+AGLDCN VTN+++QAPMVLN
Sbjct: 421 KTTFLMRLGYMENSDEMMKALKQFRGRGDQLQERFDCLVQAGLDCNTVTNMVKQAPMVLN 480

Query: 551 QSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSMYRWLRDKGAAKPNLSL 603
           Q+KDVI++KIDCLRN L YPL++VVAFPAYLCY++ERI+ RFSMY WLR++GAAKP LSL
Sbjct: 481 QTKDVIEKKIDCLRNYLAYPLESVVAFPAYLCYDMERISQRFSMYVWLRERGAAKPTLSL 540

BLAST of Sgr020808 vs. ExPASy TrEMBL
Match: A0A7N2KM00 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.9e-218
Identity = 384/577 (66.55%), Postives = 458/577 (79.38%), Query Frame = 0

Query: 56  MLILRKHERVLKFSKMIAQLNHLLLFAPVVSEK-IITHNSSFVSFKSRFFCNCGLDFRPK 115
           ML+ R H+RV +FS+MIA+LNH +LF+ V  +K II  N SF+ FK R F    L    K
Sbjct: 1   MLLSRNHKRVSEFSEMIAKLNHSVLFSHVGYDKPIIRQNPSFILFKVRSFSTSTLTNDAK 60

Query: 116 ASLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLISR 175
            S+ E ++SP+ + R SRIA+ EAQ+VLFDYLHCTRS SFTDAEHISKNSP FLQ LIS+
Sbjct: 61  VSVTELAKSPNFTPRFSRIAKNEAQNVLFDYLHCTRSFSFTDAEHISKNSPRFLQTLISK 120

Query: 176 LDGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHV 235
           +D +K V  SLTK+LRYNPINEFEPFFESLGL PSEL + LPRHLMFLSDDH+ML+NFHV
Sbjct: 121 IDTDKHVAHSLTKFLRYNPINEFEPFFESLGLRPSELSVLLPRHLMFLSDDHVMLDNFHV 180

Query: 236 LCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIGC 295
           LC YGIPRSK+GKMYKEAKEIF YD G+LA KL+AYENLGL K TVIKL+SCCPSLLIG 
Sbjct: 181 LCEYGIPRSKIGKMYKEAKEIFGYDYGILALKLQAYENLGLSKSTVIKLISCCPSLLIGG 240

Query: 296 ISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFKS 355
           ++ EF KVLE+L  L  E DW+GG +S K+TYNWNRMLDT+DFL KVGYT+ QMH+LF +
Sbjct: 241 VNCEFVKVLERLKELRFENDWIGGCLSGKSTYNWNRMLDTVDFLDKVGYTQEQMHHLFNT 300

Query: 356 NPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDIG 415
           NP++L E SGKKVY+L GRL+KL LK++E Y +FIQNP ILS K +KN+L+A+DFL +I 
Sbjct: 301 NPAILFEGSGKKVYILFGRLLKLDLKLNEIYSLFIQNPNILSVKCIKNLLRAMDFLLEIE 360

Query: 416 LRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESL--------------------- 475
           + TE IA I+S HI LLGS TLKGP+++CKELKVG++ L                     
Sbjct: 361 METEYIASIISNHIQLLGSRTLKGPRSVCKELKVGRDGLCQIIKEDPLKLFNLVSKSKIK 420

Query: 476 --------YPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLDC 535
                    P+KH EKTTFL +LGY+ENSDEM KALKQFRGRGDQLQERFDCLV+AGLD 
Sbjct: 421 SNEQESSQVPSKHQEKTTFLSRLGYMENSDEMTKALKQFRGRGDQLQERFDCLVQAGLDY 480

Query: 536 NVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSMY 595
           N+VTNI++QAP VLNQSKDVI+ KID LRN LGYPL++VV+FPAYL Y++ERI+ RFSMY
Sbjct: 481 NIVTNIVKQAPTVLNQSKDVIERKIDYLRNYLGYPLESVVSFPAYLGYDMERISQRFSMY 540

Query: 596 RWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
            WLR++GAAKP LSLS++LACSD RFVKYFVDVHPEG
Sbjct: 541 VWLRERGAAKPTLSLSTILACSDTRFVKYFVDVHPEG 577

BLAST of Sgr020808 vs. TAIR 10
Match: AT4G19650.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 561.2 bits (1445), Expect = 1.0e-159
Identity = 298/578 (51.56%), Postives = 379/578 (65.57%), Query Frame = 0

Query: 54  LAMLILRKHERVLKFSKMIAQLNHLLLFAPVVSEKIITHNSSFVSFKSRFFCNCGLDFRP 113
           +AML+ R   RV K   MI+ LN+ + F                                
Sbjct: 30  VAMLLSRNQRRVHKLLNMISNLNYCITF-------------------------------- 89

Query: 114 KASLIESSQSPSTSGRVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLIS 173
                      S+  R + + R +A  V  +  +    L++   EHISKNSP F+  L+S
Sbjct: 90  -----------SSIPRQNPVQRLKAVFVRINLSYNNTRLTY-QLEHISKNSPCFMSTLLS 149

Query: 174 RL-DGEKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENF 233
           ++ D +KDV + LTK+LRYNPINEFEPFFESLGL P E   FLPR LMFLSDD +M ENF
Sbjct: 150 KIDDNQKDVSKGLTKFLRYNPINEFEPFFESLGLCPYEFETFLPRKLMFLSDDGIMFENF 209

Query: 234 HVLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLI 293
           H LCNYGIPR K+G+MYKEA+EIF Y+ G+LA KLR YENLGL K TVIKLV+ CP LL+
Sbjct: 210 HALCNYGIPRGKIGRMYKEAREIFRYESGMLAMKLRGYENLGLSKATVIKLVTSCPLLLV 269

Query: 294 GCISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLF 353
           G I  EF  V++KL  L +  DW+G Y+S + TY+W R+L+T++FL KVG  E ++ +L 
Sbjct: 270 GGIDAEFSSVVDKLKGLQVGCDWLGRYLSDRKTYSWRRILETIEFLDKVGCKEEKLSSLL 329

Query: 354 KSNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFD 413
           K+ P+L++E SGKK YVL GRL K GL+++E Y +FI NPE+LS K VKNI K +DFL  
Sbjct: 330 KTYPALVIEGSGKKFYVLFGRLFKAGLQVNEIYRLFIDNPEMLSDKCVKNIQKTLDFLIA 389

Query: 414 IGLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESLY------------------ 473
           I + T+ I  I+  H+ L+GSC+L  P+T C  L V ++ L                   
Sbjct: 390 IRMETQFITKILLSHMELIGSCSLPAPRTACLSLNVKQDELCKILKKEPLRLFCFVSTTK 449

Query: 474 ----------PAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLD 533
                       K+LEKT FLL+LGY+ENSDEM KALKQFRGRGDQLQERFDCLVKAGL+
Sbjct: 450 KRKSKPLSEDSRKYLEKTEFLLRLGYVENSDEMVKALKQFRGRGDQLQERFDCLVKAGLN 509

Query: 534 CNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINTRFSM 593
            NVVT IIR APM+LN SKDVI++KI  L   LGYP++++V FPAYLCY+++RI+ RFSM
Sbjct: 510 YNVVTEIIRHAPMILNLSKDVIEKKIHSLTELLGYPIESLVRFPAYLCYDMQRIHHRFSM 563

Query: 594 YRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           Y WLR++ AAKP LS S++L C DARFVKYFV+VHPEG
Sbjct: 570 YLWLRERDAAKPMLSPSTILTCGDARFVKYFVNVHPEG 563

BLAST of Sgr020808 vs. TAIR 10
Match: AT5G45113.1 (mitochondrial transcription termination factor-related / mTERF-related )

HSP 1 Score: 375.6 bits (963), Expect = 8.0e-104
Identity = 187/402 (46.52%), Postives = 265/402 (65.92%), Query Frame = 0

Query: 228 MLENFHVLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCC 287
           M ENFHVLC YGIPR K+G++YKEA+EIF Y+ G+LASKL  YE L L K  VIKLV+CC
Sbjct: 1   MFENFHVLCYYGIPRDKIGRLYKEAREIFVYENGVLASKLEPYEILVLRKAIVIKLVTCC 60

Query: 288 PSLLIGCISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQ 347
           P LL+G I  EF  V+ KL  L +  DW+  Y+S + TYNW R+L+TM+ L KVG+ E +
Sbjct: 61  PLLLVGGIDCEFVSVVNKLKGLNLGCDWLARYLSVRKTYNWRRILETMELLEKVGFKEKK 120

Query: 348 MHNLFKSNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAV 407
           + NL K+ P L+ E SG K Y++  +  K+GL+M+E   + I N E+L  K VK IL+A+
Sbjct: 121 LSNLLKAYPDLVGETSGNKAYIMFEKFHKVGLQMNEIDKLLIDNSEMLLEKSVKRILEAL 180

Query: 408 DFLFDIGLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESL-------------- 467
            FL  I +  + +   +  H+  + S +L  P+ +   LK+ ++ L              
Sbjct: 181 KFLKCIRIEKQFVVRFLQCHMKHICSSSLLVPRAVWNRLKIRRDELCQIIKEEPLRLFSI 240

Query: 468 -------------YPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVK 527
                          +++ EKTTFLLKLGY+ENSDEM +ALK+F+GRGD+LQERFDC VK
Sbjct: 241 ASKTNKGRIELDSLDSRNAEKTTFLLKLGYVENSDEMVRALKKFQGRGDELQERFDCFVK 300

Query: 528 AGLDCNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVERINT 587
           AGLD NVV+ ++++AP +LN+ KD+I++KI  L + L YP+++V+  P YLCY+++RI+ 
Sbjct: 301 AGLDYNVVSQLVKRAPHILNRPKDIIEKKIIMLIDYLVYPIESVIESPTYLCYSMKRIHQ 360

Query: 588 RFSMYRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
           RF+MY WLR++ A  P L+L +V+  S+   V YFV+ HPEG
Sbjct: 361 RFTMYIWLRERDAVIPRLTLGTVVGISNTLIVPYFVNTHPEG 402

BLAST of Sgr020808 vs. TAIR 10
Match: AT5G06810.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 372.5 bits (955), Expect = 6.8e-103
Identity = 208/531 (39.17%), Postives = 308/531 (58.00%), Query Frame = 0

Query: 111  FRPKASLIESSQSPSTSG-RVSRIARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQ 170
            F  + +L+++  S    G R     R  AQ  +FDY + TR L F  AE +SKN+P F  
Sbjct: 598  FATQRALVDAEVSGEKWGLRTRNEIRKVAQVAMFDYFYQTRGLQFLVAESMSKNAPVFND 657

Query: 171  HLISRLDG-------EKDVGRSLTKYLRYNPINEFEPFFESLGLPPSELPLFLPRHLMFL 230
            +L+ +L+G       + D+ +++T++L ++P+NEFEPF ESLGL PSE    +P   MFL
Sbjct: 658  NLLKKLNGCDVDVDDDDDIVKAITRFLWFHPVNEFEPFLESLGLKPSEFSHLIPCDKMFL 717

Query: 231  SDDHLMLENFHVLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIK 290
            ++D  +LEN+HV  NYGI R K+GK++KEA+E+F Y+ G+LASK+++YE+LG  K  + K
Sbjct: 718  NEDAFLLENYHVFWNYGIGREKMGKIFKEAREVFGYETGVLASKIKSYEDLGFSKLFLSK 777

Query: 291  LVSCCPSLLIGCISMEFFKVLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVG 350
            L+ C PS+LIG +++   KV+E L  +G   DWV   +S + +Y+W+ M   + FL  + 
Sbjct: 778  LIVCSPSILIGDMNVGLAKVMEMLKAIGFGVDWVTENLSEEVSYDWSSMHRCLSFLRDLY 837

Query: 351  YTEIQMHNLFKSNPSLLLEDSGKKVYVLLGRLVKLGLKMDEAYLMFIQNPEILS-GKYVK 410
              E ++  L +  P L+ EDSG+   +L G   KLG    E   +F + P+  S GK+V 
Sbjct: 838  VDENELCELIRKMPRLIFEDSGEWTLILAGFEAKLGSSRSELSSLFQKFPQCQSLGKFVL 897

Query: 411  NILKAVDFLFDIGLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKESL-------- 470
            N+     FL DI +  ++I  I   H L +G   LK   T+   LK GK  L        
Sbjct: 898  NLRHCFLFLKDIEMDDDEIGKIFRLHSLWIGVSRLKQTSTLLINLKGGKGRLCQVIQENP 957

Query: 471  --------------YPAKHLE--------KTTFLLKLGYIENSDEMAKALKQFRGRGDQL 530
                           PA   +        KT FLL LGY ENS+EM +ALK FRG+G +L
Sbjct: 958  EEMKKWIMGLRVQPLPATGYKVNTKSKTMKTQFLLDLGYKENSEEMERALKNFRGKGSEL 1017

Query: 531  QERFDCLVKAGLDCNVVTNIIRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYL 590
            +ERF+ LV  GL    V ++++  P +L Q+ D+++ K++ L   LGYPL T+V FP  L
Sbjct: 1018 RERFNVLVSFGLTEKDVKDMVKACPSILTQACDILESKVNYLVKELGYPLSTLVTFPTCL 1077

Query: 591  CYNVERINTRFSMYRWLRDKGAAKPNLSLSSVLACSDARFVKYFVDVHPEG 603
             Y ++R+  RFSM+ WL+D+G A P L +S++L CSD  F   FV+ HP+G
Sbjct: 1078 KYTLQRMKLRFSMFSWLQDRGKADPKLQVSTILVCSDKFFATRFVNRHPDG 1128

BLAST of Sgr020808 vs. TAIR 10
Match: AT3G60400.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 237.7 bits (605), Expect = 2.6e-62
Identity = 159/510 (31.18%), Postives = 261/510 (51.18%), Query Frame = 0

Query: 133 IARTEAQDVLFDYLHCTRSLSFTDAEHISKNSPHFLQHLISRLD-GEKDVGRSLTKYLRY 192
           +A  +AQ  + DYLH TRSLS+T AE I+ N+   +++LI +LD       +SL K+L Y
Sbjct: 32  LAIGKAQQAITDYLHTTRSLSYTHAEQIASNASVSIRNLILKLDFSVPTFSKSLRKHLSY 91

Query: 193 NPINEFEPFFESLGLPPSELPLFLPRHLMFLSDDHLMLENFHVLCNYGIPRSKLGKMYKE 252
           +PINEFE FFES+G+  SE+  FLP    F S+D  +L+    L  +G P +KLGK+YKE
Sbjct: 92  HPINEFEFFFESIGIDYSEVSEFLPEKKFFFSEDRTVLDAAFALSGFGFPWNKLGKLYKE 151

Query: 253 AKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLLIGC--ISMEFFKVLEKLSRL 312
            + +F    G + S+L  ++++G     VI      P  L G   +  E   +  KL RL
Sbjct: 152 ERLVFVQRPGEIESRLLKFKDIGFSTVAVIGTCLAIPRTLCGGGELGSEIRCLFVKLKRL 211

Query: 313 GIEKDWVGGYISCKNTYNWNRMLDTMDFLAKVGYTEIQMHNLFKSNPSLLLEDSGKKVYV 372
             E D    ++  +N  +W  +   +     +G    +M  L   N SL LE S + +  
Sbjct: 212 FDEFD--SHHLFEENVDSWLAVSRKIRIFYDLGCENEEMWELMCRNKSLFLEYSEEALMN 271

Query: 373 LLGRLVKLGLKMDEAYLMFIQNPEILSGKYVKNILKAVDFLFDIGLRTEDIACIVSQH-- 432
             G   + G+  ++A L+ ++NP I++    K ++     L   GLR +++  +  ++  
Sbjct: 272 KAGYFCRFGVSKEDAALLILRNPAIMNFDLEKPVISVTGMLKHFGLRQDEVDAVAQKYPY 331

Query: 433 -------------------------IL------LLGSCTLKGP-KTICKELKVGKESLYP 492
                                    IL      LL S TL  P + + +E + G E L  
Sbjct: 332 VFGRNQLKNLPYVLRAIDLHERIFDILKNGNHHLLASYTLMDPDEDLEREYQEGLEELQN 391

Query: 493 AK----HLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLDCNVVTNI 552
           ++    +++K  FL ++G+ EN   M K L+   G   +L +RF  L+ +G+  + +  +
Sbjct: 392 SRTKRHNIQKLDFLHEIGFGENGITM-KVLQHVHGTAVELHDRFQILLNSGIIFSKICML 451

Query: 553 IRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVE-RINTRFSMYRWLRD 601
           IR AP +LNQ    IQ+K+  L   +G  LD +  FPAYLC+++E RI+ RF  ++WL +
Sbjct: 452 IRSAPKILNQKPHSIQDKLRFLCGEMGDSLDYLEVFPAYLCFDLENRISPRFRFHKWLVE 511

BLAST of Sgr020808 vs. TAIR 10
Match: AT1G74120.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-13
Identity = 87/397 (21.91%), Postives = 164/397 (41.31%), Query Frame = 0

Query: 233 HVLCNYGIPRSKLGKMYKEAKEIFSYDCGLLASKLRAYENLGLGKGTVIKLVSCCPSLL- 292
           ++L  YG P S L         + + D       L    +L + + +++ L+S CP++L 
Sbjct: 46  NLLQRYGFPPSSLQHFLSRNNHLLNSDLVETEISLGILLSLKIPQKSLVSLISDCPNVLR 105

Query: 293 ----------------IGCISMEFFK-VLEKLSRLGIEKDWVGGYISCKNTYNWNRMLDT 352
                            G +S    K VLE  SR+GI  D               +  + 
Sbjct: 106 SEFLRKWRVPLSNCGKHGVVSSSAIKSVLEHSSRIGIGPD---------------KFNEC 165

Query: 353 MDFLAKVGYTEIQMHNLFKSNPSLLLEDS---GKKVYVLLGRLVKLGLKMDEAYLMFIQN 412
           +  L  +G+ +  +  +  S P +LL +     +K+  L+G    +G+  D     F   
Sbjct: 166 VRVLKSLGFCDSTVSRILSSFPGVLLVNEIEIRRKIEFLVG----IGIARDNIERFFHVF 225

Query: 413 PEILSGKYVKNILKAVDFLFDIGLRTEDIACIVSQHILLLGSCTLKGPKTICKELKVGKE 472
           PE+L       +   +D    +G   +D+   +++   +LG    + P+  C EL     
Sbjct: 226 PEVLGIGTETRLKPLLDEFMKMGFSKDDVKKEIAREPRVLGLELGELPR--CLEL----- 285

Query: 473 SLYPAKHLEKTTFLLKLGYIENSDEMAKALKQFRGRGDQLQERFDCLVKAGLDCNVVTNI 532
                 +  K   ++++  I            FR  G +++ R DCL K GL       +
Sbjct: 286 -----INTLKCREVIRVSIISEG--------AFRA-GFEVKLRVDCLCKYGLIRRDAFKV 345

Query: 533 IRQAPMVLNQSKDVIQEKIDCLRNCLGYPLDTVVAFPAYLCYNVER-INTRFSMYRWLRD 592
           + + P V+    + I++KI+ L N +G+ ++ +   P YL  N+++ I  R+++  +L+ 
Sbjct: 346 VWKEPRVILYEIEDIEKKIEFLTNRMGFHINCLADVPEYLGVNLQKQIVPRYNVIDYLKL 402

Query: 593 KGAAKPNLSLSSVLACSDARFVKYFVDVHPEGLLRFG 608
           KG    ++ L  ++  S  RF   +V  +PE    FG
Sbjct: 406 KGGLGCDIGLKGLIKPSMKRFYNLYVMPYPECERIFG 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142385.11.4e-29186.81transcription termination factor MTEF18, mitochondrial [Momordica charantia][more]
XP_023000703.14.6e-27181.83transcription termination factor MTEF18, mitochondrial-like [Cucurbita maxima][more]
XP_022927279.12.3e-27081.49transcription termination factor MTEF18, mitochondrial-like [Cucurbita moschata][more]
XP_023519412.15.1e-27081.49transcription termination factor MTEF18, mitochondrial-like [Cucurbita pepo subs... [more]
XP_038893610.18.7e-27080.28transcription termination factor MTEF18, mitochondrial [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9M2193.7e-6131.18Transcription termination factor MTEF18, mitochondrial OS=Arabidopsis thaliana O... [more]
Q9C6A13.1e-1221.91Transcription termination factor MTERF15, mitochondrial OS=Arabidopsis thaliana ... [more]
Q9FK233.0e-0723.39Transcription termination factor MTERF8, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1CKT46.7e-29286.81transcription termination factor MTEF18, mitochondrial OS=Momordica charantia OX... [more]
A0A6J1KND22.2e-27181.83transcription termination factor MTEF18, mitochondrial-like OS=Cucurbita maxima ... [more]
A0A6J1EHL01.1e-27081.49transcription termination factor MTEF18, mitochondrial-like OS=Cucurbita moschat... [more]
A0A2N9ISM12.2e-21867.79Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55025 PE=3 SV=1[more]
A0A7N2KM002.9e-21866.55Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G19650.11.0e-15951.56Mitochondrial transcription termination factor family protein [more]
AT5G45113.18.0e-10446.52mitochondrial transcription termination factor-related / mTERF-related [more]
AT5G06810.16.8e-10339.17Mitochondrial transcription termination factor family protein [more]
AT3G60400.12.6e-6231.18Mitochondrial transcription termination factor family protein [more]
AT1G74120.12.2e-1321.91Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 509..540
e-value: 5.6
score: 15.9
coord: 541..574
e-value: 140.0
score: 10.5
coord: 385..416
e-value: 830.0
score: 4.5
coord: 349..380
e-value: 3000.0
score: 0.2
coord: 246..276
e-value: 770.0
score: 4.8
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 489..591
e-value: 1.7E-14
score: 53.5
coord: 324..432
e-value: 1.1E-8
score: 34.4
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 456..606
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 68..454
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 180..366
e-value: 3.3E-13
score: 51.4
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 367..607
e-value: 3.7E-46
score: 159.4
NoneNo IPR availablePANTHERPTHR13068:SF103MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEINcoord: 68..454
NoneNo IPR availablePANTHERPTHR13068:SF103MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEINcoord: 456..606

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr020808.1Sgr020808.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0032502 developmental process
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0009507 chloroplast
molecular_function GO:0003690 double-stranded DNA binding