Sgr020681 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr020681
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Serine hydroxymethyltransferase
Location	tig00153554: 143567 .. 148157 (+)
RNA-Seq Expression	Sgr020681
Synteny	Sgr020681

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTGTCCAATCCCCATTCCAATCTATCTCTTGGATTTTCGTCACATGCTTCGCCACCCCACCGCGCTCAAATTGCTGATGATTCCATCCTGCTTCATCTCGATTCTACTCGACGGGAACCCGCGTATTCGTCCCCCCCAGTCCCTCTTCAGTTGATGGAACCGCAGACGGAGAGTCACAGAGACGAGGATGGAGATGCGGAGAGAGAAGACGATGACTACGACGACAGAGAGGTAGAAGAGTTTCGTATTCTGGGTCATTCTATGTGCTTGAAGCGCCGAAGAGATGGCGAATCTTCATCCTCGTCGGGCTCTACGAAGAGAATTTCGTGTGAATTTGATATGGAGACACGAAGGAGTGCTGTACGGTCGTGGGGTAACCAGAACCTTCGAGTTGCGGACCCTAACCTATTTGGGATTATGGAGAAGGAGAGACAGAGGCAATTCAAGGGCATTGAATTGATTGCTTCCGAAAATTATGTGTGCCGGGCTGTGATGGAAGCACTTGGAAGCCATTTGACTAATAAGTACTCGGAAGGAATGCCTGGTGCGCGTTATTACTGTGGAAATCAATATATAGATGAGATTGAAACCCTGTGTCGTGAGCGCGCATTGGCGGCCTTTGGTCTCGATTCTGATAGTTGGGGAGTTAACGTGCAACCTTATTCATGCACGTCCGCGAACTTTGCTGTCTACACTGGTCTGTTACTACCCGGCGATCGAATCATGGGGTTGGATACTCCTTCTGGTGGGAATACAAGCCATGGCTACTGTACCCCAAATGGCAGAAAAGTTTCTGGGGCCTCAATTTTCTTTGAGAGCTTGCCATATAAGGTGAATCCGCAAACTGGTTATATCGATTATGACAAGCTCGAGGAGAGGGCGCTTGACTTTCGCCCAAAAATACTTATTTGTGGTGGGAGCTCATATCCTCGTGAATGGGATTATGCAAGGTTTAGGCAGATTGCAGATAAGTGTGGAGCAGTTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCGAAGGTGTGAATTTTTCTCCATCTTTAGCAATGATACGGCCCTGGATTATCTGTATCTTTTTTGGGTTAATCTGCTCAGCAAAGTTTTCGGTTTTCTATGTAATATTGTGTCTTCGTTCTCTTGGGTCACGATTTAGTTGGTTGTTTATATAGTCAAGCCATTTGAATATTCTTACTTGACACTCAGAAAAGGCTTCTAAGGTGGTTGTGTGGGAAGTTCATTGTAGTTTTTGATATTTATGATATGTATGGCTATGAAACTTAGGGATAAGGAAATGTATTTGATAAAGGTATTTTGGTAGACATTGATTTTAGGTCTTACCCCCCATTTTAGAAGTGTGCTTGGCCAATACCTCTGGGTACTTATCCTTTCATAGTGGATATGTATTTAACAGGAAAACCGACCTGGGTTGTATTGTTCTTTCTCAGGAGGACAATGTTACCATATTCCCTCAATATTTAACCAAAGCTTTATTTTCCCAGTGAGAACTGATATCTTAGAGAAACACTTGTGAATGCTTTTGGATCTTTAAGCTTATGAATTGTGTATTATTTTTCTTTTCTACTTTTTATCTAAAGATTATTTTTCCTTTCTACTTGTTTCTATAATGCAGTTTCGTTGAACTGTGTATTTATGCATGTTTCACCTTTTTCCGGGGGGGTGGGAGGGGAGTGATTTTATCTTTTGGTCTGCATGTCATTACAGTATGTTTGTAGAACCTTTTTGTAACTTTTTTATGGTGGAGTGCATGTTTTGACGCTTGTTATCTAGTTACTTCTGTTTACACTTTTATTACTCATAGTGAATTGTTGAATCCTTTTTTAATAAAAATAGTTTGTTGCTTTTAGATCTGTAATTTTTTTTCCACTTACATTGTTTGGTCCATCAGTATTACGTATGTGTAAACTTGTAGTTTTAGATGGAGAATTATCCCTACTTTTTCATTAATATTTTGATTTGAATTTTCTTACAGGAATGTGTTAGCCCTTTTGGATACTGTGATGTTGTAACTTCAACTACTCATAAGAGCCTCCGAGGTCCAAGGGGAGGTATAATTTTTTATAGGAAGGGTAGCAAGCCAAGGAAGAGAGCAATGCTTCTGTATCAGGGAAATGAAACTGACCAATATGACTTTGAGGAGAAGATAAACTTTGCTGTTTTCCCTTCTTTACAAGGTGGACCACACAATAACCATATTGCTGCTCTTGCCGTGGCCCTAAAACAAGTGGATACTCCAGAATACAAGGCATATATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTGGCTTCTGCTTTACTTCGAAAGAAGCACAGGCTTGTAACTGGTGGCACGGATAATCACATGTTACTTTGGGATCTAAGGCCCTTGGGATTGACAGGTGCATAATCTTGATTCAAATTTTGCAAATTGATGTGAACATGACATATTTGAGGCGCACGGCAAGCTTTTTATTTGTCTAGACACAAGAGGCGCACTAAAAGTGTTGCTCAAGTAAAGTGAGGGTAGAGATTATGTTATGCATTGTGAGTTGTGATTAGAGGAAATAGAATATTATTACATAATATGAGTTACATAATTTGGAATAATATATCTTTTCTATCAACTTTATAACAATATAAAATAGGATATTTTAGTTAAATATGGTACTTGACTAGGCGCAAATAAGTTTTTGTCAAATACATTATCTATAGTAGCCAATCCGGATATAGCTCAATGGATAAGGCACTTATTACCATCTCAAAGGTTGATGGTTCGATCCTTCACCCCTACAAATGTTGAACTAAAAAAAGTAGTAATAGTAGAGGTTGACGTCTAATACATTAATAAGAAAGCTGAATATATTTTTTAAAATTAGTTGGTTGTTAGAAGTGATAAAAAGTTGACTAAGGCATGCAATTGTCAGGCATGGCAAGTCTTAGCACCATCTCGAAAACACACCAAACGAATGGAAAAGTACAGAAAGGGTGATGAGAAAATAACCCAAAGAGCTAAGAGGATGTGGAAAAGTTTTCCCTATTGGCATTGATAAATAATGATTGCAGTTACGAAAAAAAATTGTCAAGGAAATCCAAGAAGCCTTGTATTGGATGAACTCCCAAATTTCTCCTCTAGCCAAGTTGTGAAACAATCTATGATTTCTTTCTAGTCAAACTTTCAAGAGTGAAGCCTTCATGGCATTTTCTCTCAGGTACTTTGCTTTATTTTTAAAGGGATGCCACGTAGGCAAATAATCCAGGTTTTGCAGAATAGAAAAAGGGAAGCATTGAGCAAAATTAAACTTATCTAGAAGAGTTCCTCAATGATTTTGGTAGAAGGAGCAGTGTACGAATAGACTGTTTTGGGATTTTGAACCATCTGTCTCGAGCAGCAACAGCTAGAAGAGACGAGGCTGAGGATTTCTTCTTTGTATAATAACAGCCGTACTGATGTCCATATAACTAGGTTCCACAGAAATATCTTGTCTTCTTAGGGCTTTCTTTCTGCCAAATGGCTCTAGAAAGCCTCTTGTTAGGAATGGTGGATCAGTTGGCTAGTTGGACAAAGAGGGAGCAACTTGTAAATGTGCCCCTATCGTCCTACTGCAAAACAATGGATTTGTAGCTCATGATTTATCTCTAGAACTACATCTTACATAGATGGGTTGCAGAGTCTATCAAGAAAGATATGGCTTGTAGTGAAATTAGTTCTTAGTATCGTTCAAATTGCGTCAAGTTTTGTCTTTCAAAGGTTGTCTCCTCTACATCCTCCTGTATTGAGCTGATACTGAGATCATAAGTTTATTTTGAAGTTATCAAATAGCTTTTTTCGAAATGAATTCATATGACTTTTCAACTGGATGCAGTACATTGATTCACTGATAAAAAGAATCCTATTATCATAGTGTGCTGTGTGTAGTGGACTGCAATGGATCTCTGGCTTCTTTGTTTATATTATACAATGTAAAAAAATGAAGATAGTTTCTGAATGATGTATAATCTATTACAACTCATAACTTGTTTGGTATTTCATATTTGTATACGTCTGCTTCTTCTAAGTTCTAGTTTTGATGGCACCTGTGGCTGCTTAATCTATTATTCTCTATTTATTATATATTGTGTAATGTTCCTGATGGCATTGTAATTGTAGGTAAGAACTATGAGAAGGTTTGTGAGATATGCCAAATTACTCTCAACAAAATTACAATATTTGGTGATAATGGAATTATTATACCTGGAGGAGTACGGATTGGTGAGTAATACCATCTTATATTTCATATTTTGGGGGAATCCTAAGATTGGAGAGCTTGGTATGCATTCATTATGCGATATAAATTAGCTTTCTGTTATCCTTGTAGGCACCCCAGCAATGACATCTAGAGGCTGTCTGGAATCTGATTTTGAGATAATTGCTGACTTTCTTCATACAGCCGCTCAAATTGCAATTTCAGTACAGCGGGAGCATGGGAAGCTACCAAAGGCTTATCTTAAGGGCCTTCAAAGCAATAATGAAGTTGTTGAGCTCCGAAACCGTGTTGAAAGTTTTTCAGCTCAGTTTGCAATGCCGGGCTTTGAAACTTGA

mRNA sequence

ATGGATTTGTCCAATCCCCATTCCAATCTATCTCTTGGATTTTCGTCACATGCTTCGCCACCCCACCGCGCTCAAATTGCTGATGATTCCATCCTGCTTCATCTCGATTCTACTCGACGGGAACCCGCGTATTCGTCCCCCCCAGTCCCTCTTCAGTTGATGGAACCGCAGACGGAGAGTCACAGAGACGAGGATGGAGATGCGGAGAGAGAAGACGATGACTACGACGACAGAGAGGTAGAAGAGTTTCGTATTCTGGGTCATTCTATGTGCTTGAAGCGCCGAAGAGATGGCGAATCTTCATCCTCGTCGGGCTCTACGAAGAGAATTTCGTGTGAATTTGATATGGAGACACGAAGGAGTGCTGTACGGTCGTGGGGTAACCAGAACCTTCGAGTTGCGGACCCTAACCTATTTGGGATTATGGAGAAGGAGAGACAGAGGCAATTCAAGGGCATTGAATTGATTGCTTCCGAAAATTATGTGTGCCGGGCTGTGATGGAAGCACTTGGAAGCCATTTGACTAATAAGTACTCGGAAGGAATGCCTGGTGCGCGTTATTACTGTGGAAATCAATATATAGATGAGATTGAAACCCTGTGTCGTGAGCGCGCATTGGCGGCCTTTGGTCTCGATTCTGATAGTTGGGGAGTTAACGTGCAACCTTATTCATGCACGTCCGCGAACTTTGCTGTCTACACTGGTCTGTTACTACCCGGCGATCGAATCATGGGGTTGGATACTCCTTCTGGTGGGAATACAAGCCATGGCTACTGTACCCCAAATGGCAGAAAAGTTTCTGGGGCCTCAATTTTCTTTGAGAGCTTGCCATATAAGGTGAATCCGCAAACTGGTTATATCGATTATGACAAGCTCGAGGAGAGGGCGCTTGACTTTCGCCCAAAAATACTTATTTGTGGTGGGAGCTCATATCCTCGTGAATGGGATTATGCAAGGTTTAGGCAGATTGCAGATAAGTGTGGAGCAGTTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCGAAGGAATGTGTTAGCCCTTTTGGATACTGTGATGTTGTAACTTCAACTACTCATAAGAGCCTCCGAGGTCCAAGGGGAGGTATAATTTTTTATAGGAAGGGTAGCAAGCCAAGGAAGAGAGCAATGCTTCTGTATCAGGGAAATGAAACTGACCAATATGACTTTGAGGAGAAGATAAACTTTGCTGTTTTCCCTTCTTTACAAGGTGGACCACACAATAACCATATTGCTGCTCTTGCCGTGGCCCTAAAACAAGTGGATACTCCAGAATACAAGGCATATATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTGGCTTCTGCTTTACTTCGAAAGAAGCACAGGCTTGTAACTGGTGGCACGGATAATCACATGTTACTTTGGGATCTAAGGCCCTTGGGATTGACAGGTAAGAACTATGAGAAGGTTTGTGAGATATGCCAAATTACTCTCAACAAAATTACAATATTTGGTGATAATGGAATTATTATACCTGGAGGAGTACGGATTGGCACCCCAGCAATGACATCTAGAGGCTGTCTGGAATCTGATTTTGAGATAATTGCTGACTTTCTTCATACAGCCGCTCAAATTGCAATTTCAGTACAGCGGGAGCATGGGAAGCTACCAAAGGCTTATCTTAAGGGCCTTCAAAGCAATAATGAAGTTGTTGAGCTCCGAAACCGTGTTGAAAGTTTTTCAGCTCAGTTTGCAATGCCGGGCTTTGAAACTTGA

Coding sequence (CDS)

ATGGATTTGTCCAATCCCCATTCCAATCTATCTCTTGGATTTTCGTCACATGCTTCGCCACCCCACCGCGCTCAAATTGCTGATGATTCCATCCTGCTTCATCTCGATTCTACTCGACGGGAACCCGCGTATTCGTCCCCCCCAGTCCCTCTTCAGTTGATGGAACCGCAGACGGAGAGTCACAGAGACGAGGATGGAGATGCGGAGAGAGAAGACGATGACTACGACGACAGAGAGGTAGAAGAGTTTCGTATTCTGGGTCATTCTATGTGCTTGAAGCGCCGAAGAGATGGCGAATCTTCATCCTCGTCGGGCTCTACGAAGAGAATTTCGTGTGAATTTGATATGGAGACACGAAGGAGTGCTGTACGGTCGTGGGGTAACCAGAACCTTCGAGTTGCGGACCCTAACCTATTTGGGATTATGGAGAAGGAGAGACAGAGGCAATTCAAGGGCATTGAATTGATTGCTTCCGAAAATTATGTGTGCCGGGCTGTGATGGAAGCACTTGGAAGCCATTTGACTAATAAGTACTCGGAAGGAATGCCTGGTGCGCGTTATTACTGTGGAAATCAATATATAGATGAGATTGAAACCCTGTGTCGTGAGCGCGCATTGGCGGCCTTTGGTCTCGATTCTGATAGTTGGGGAGTTAACGTGCAACCTTATTCATGCACGTCCGCGAACTTTGCTGTCTACACTGGTCTGTTACTACCCGGCGATCGAATCATGGGGTTGGATACTCCTTCTGGTGGGAATACAAGCCATGGCTACTGTACCCCAAATGGCAGAAAAGTTTCTGGGGCCTCAATTTTCTTTGAGAGCTTGCCATATAAGGTGAATCCGCAAACTGGTTATATCGATTATGACAAGCTCGAGGAGAGGGCGCTTGACTTTCGCCCAAAAATACTTATTTGTGGTGGGAGCTCATATCCTCGTGAATGGGATTATGCAAGGTTTAGGCAGATTGCAGATAAGTGTGGAGCAGTTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCGAAGGAATGTGTTAGCCCTTTTGGATACTGTGATGTTGTAACTTCAACTACTCATAAGAGCCTCCGAGGTCCAAGGGGAGGTATAATTTTTTATAGGAAGGGTAGCAAGCCAAGGAAGAGAGCAATGCTTCTGTATCAGGGAAATGAAACTGACCAATATGACTTTGAGGAGAAGATAAACTTTGCTGTTTTCCCTTCTTTACAAGGTGGACCACACAATAACCATATTGCTGCTCTTGCCGTGGCCCTAAAACAAGTGGATACTCCAGAATACAAGGCATATATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTGGCTTCTGCTTTACTTCGAAAGAAGCACAGGCTTGTAACTGGTGGCACGGATAATCACATGTTACTTTGGGATCTAAGGCCCTTGGGATTGACAGGTAAGAACTATGAGAAGGTTTGTGAGATATGCCAAATTACTCTCAACAAAATTACAATATTTGGTGATAATGGAATTATTATACCTGGAGGAGTACGGATTGGCACCCCAGCAATGACATCTAGAGGCTGTCTGGAATCTGATTTTGAGATAATTGCTGACTTTCTTCATACAGCCGCTCAAATTGCAATTTCAGTACAGCGGGAGCATGGGAAGCTACCAAAGGCTTATCTTAAGGGCCTTCAAAGCAATAATGAAGTTGTTGAGCTCCGAAACCGTGTTGAAAGTTTTTCAGCTCAGTTTGCAATGCCGGGCTTTGAAACTTGA

Protein sequence

MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTESHRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRRSAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLTGKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAAQIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFET

Homology

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BLAST of Sgr020681 vs. NCBI nr
Match: XP_022149204.1 (serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149205.1 serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149207.1 serine hydroxymethyltransferase 7-like [Momordica charantia])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 567/587 (96.59%), Postives = 577/587 (98.30%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLSN HSNLSLGFSSHASPPHRAQIADDS+LLHLDSTRREP YSSPPVPLQLMEPQTES
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HRDEDG+AEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKR+SCEFDM+ RR
Sbjct: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQ LRVADPNLFGIMEKE+ RQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGLDSD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+ TPEYKAYMQQVKKNAQALASALLRKK+RLVTGGTDNHMLLWDLRPLGLT
Sbjct: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKNYEKVCEICQITLNKITI+GDNGIIIPGGVRIGTPAMTSRGCLESDFE I DFLHTAA
Sbjct: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAISVQREHGKLPKAYLKGLQ+NNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of Sgr020681 vs. NCBI nr
Match: XP_022941930.1 (serine hydroxymethyltransferase 7-like [Cucurbita moschata] >KAG6600365.1 Serine hydroxymethyltransferase 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031023.1 Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 552/587 (94.04%), Postives = 570/587 (97.10%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLS  HSNLSLGFSSHASPPHRAQIADDS+LLHLDS+RRE +YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HR++ GDAER+DD+YDDREVEEFRILGHSMCLKRRRDGESS+SSGS KRISCEFD+ETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHSMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+Q LRVADPNLFGIMEKE+QRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQV TPEYKAYMQQVKKNAQALA+ALLRKK++LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKNYE VCEIC ITLNKITIFGDNGIIIPGGVR+GTPAMTSRGCLESDFE IADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAISVQRE+GKLPK YLKGL SNNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of Sgr020681 vs. NCBI nr
Match: XP_023547556.1 (serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 550/587 (93.70%), Postives = 568/587 (96.76%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLS  HSNLSLGFSSHASPPHRAQIADDS+LLHLDS+RRE +YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HR++ GD ER+DD+YDDREVEEFRILGH MCLKRRRDGESS+SSGS KRISCEFD+ETRR
Sbjct: 61  HRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+Q LRVADPNLFGIMEKE+QRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQV TPEYKAYMQQVKKNAQALA+ALLRKK++LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKNYE VCEIC ITLNKITIFGDNGIIIPGGVR+GTPAMTSRGCLESDFE IADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAISVQRE+GKLPK YLKGL SNNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of Sgr020681 vs. NCBI nr
Match: XP_022979958.1 (serine hydroxymethyltransferase 7-like [Cucurbita maxima])

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 549/587 (93.53%), Postives = 567/587 (96.59%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLS  HSNLSLGFSSHASPPHRAQIADDS+LLHLDS+RRE +YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HR++ GDAER+DD+YDDREVEEFRILGH MCLKRRRDGESS+SS S KRISCEFD+ETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+Q LRVADPNLFGIMEKE+QRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQV TPEYKAYMQQVKKNAQALA+ALLRKK++LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKNYE VCEIC ITLNKITIFGDNGIIIPGGVR+GTPAMTSRGCLESDFE IADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAISVQRE+GKLPK YLKGL SNNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of Sgr020681 vs. NCBI nr
Match: KAG6576983.1 (Serine hydroxymethyltransferase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015003.1 Serine hydroxymethyltransferase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 533/588 (90.65%), Postives = 554/588 (94.22%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLSN HSNLSLG SSHASPPHR QIADDSILLHL+S+R+EP +SSPP+PLQLMEP TES
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HRDE GD EREDD YDDR+VEEFRILGHSMCLKRRRD ESS SS S KR SCE D+ETRR
Sbjct: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQ LR ADPNLFGIMEKE+QRQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQV T EYKAYMQQVKKNAQALASALLRK ++LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKN+E+VCE+C ITLNKITIFGDNGII PGGVRIGTPAMTSRGCLESDFE+I +FLH AA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFET 589
           QIAI VQREHGKLPKA+L+GLQSNNEVVEL  RV SFSA+FAMPG ET
Sbjct: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 588

[Close]

BLAST of Sgr020681 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.6e-239
Identity = 413/591 (69.88%), Postives = 478/591 (80.88%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFS-SHAS----PPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLME 60
           MDLS   +N  LGF  SHAS    P  RA IADDSI L +D + R    +  P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  PQTESHRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGE-SSSSSGSTKRISC-E 120
            + E     D   +      D +E E FRILGH MCLKR+RD     + S   KR S  +
Sbjct: 61  QKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGD 120

Query: 121 FDMETRRSAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSH 180
            D+E+RR+AVR+WG+Q + +ADP++  +MEKE+QRQ +GIELIASEN+VCRAVMEALGSH
Sbjct: 121 SDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSH 180

Query: 181 LTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVY 240
           LTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL+SD WGVNVQPYSCTSANFAVY
Sbjct: 181 LTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVY 240

Query: 241 TGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLE 300
           TGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYKVNPQTGYIDYDKLE
Sbjct: 241 TGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLE 300

Query: 301 ERALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGY 360
           ++ALD+RPKILICGGSSYPR+WD+AR RQIADKCGAVLMCDMA ISGLVA KEC +PF +
Sbjct: 301 DKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDH 360

Query: 361 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQG 420
           CD+VTSTTHK LRGPRGGIIFYR+G K RK+       + +  YD EEKINFAVFPSLQG
Sbjct: 361 CDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQG 420

Query: 421 GPHNNHIAALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWD 480
           GPHNNHIAALA+ALKQV TPEYKAY+QQ+KKNAQALA+ALLR+K RLVTGGTDNH+LLWD
Sbjct: 421 GPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWD 480

Query: 481 LRPLGLTGKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIA 540
           L P+GLTGK YEKVCE+C ITLNK  IFGDNG I PGGVRIGTPAMT+RGC+ESDFE +A
Sbjct: 481 LTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMA 540

Query: 541 DFLHTAAQIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMP 585
           DFL  AAQI  ++QREHGK  K ++K L +N ++ ELRNRVE+F+ Q+ MP
Sbjct: 541 DFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

[Close]

BLAST of Sgr020681 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 3.9e-238
Identity = 408/592 (68.92%), Postives = 484/592 (81.76%), Query Frame = 0


Query: 8   SNLSLGF-SSHASP---PHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTESHR- 67
           S+LSLGF SSHA P   P R  IADDSI L +DS+ R  +   PPVPLQL+E + +    
Sbjct: 7   SDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVTGS 66

Query: 68  -----DEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSS--SSGSTKRISCEFD 127
                +ED +   ++DD D RE E+F +LGH M LKR R G S S  SS   KR   +  
Sbjct: 67  CSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDSG 126

Query: 128 METRRSAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLT 187
           +E+RR+ VR+WGNQ++  ADP +   MEKE+QRQF+GIELIASEN+VCRAVMEALGSHLT
Sbjct: 127 IESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLT 186

Query: 188 NKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTG 247
           NKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSANFAV+TG
Sbjct: 187 NKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTG 246

Query: 248 LLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEER 307
           LL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYKV+P+TGYIDYDKLEE+
Sbjct: 247 LLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEK 306

Query: 308 ALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCD 367
           ALD+RPKILICGGSSYPR+W++ RFR IADKCGAVLM DMAQISGLVAAKE  +PF YCD
Sbjct: 307 ALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCD 366

Query: 368 VVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGP 427
           +VTSTTHKSLRGPRGGIIFY++G KP+K+++ L       QYDFEEKINF+VFPSLQGGP
Sbjct: 367 IVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGP 426

Query: 428 HNNHIAALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLR 487
           HNNHIAALA+ALKQ  +PEYK YM+QVKKNA+ALASAL+ +K +L+TGGTDNH+LLWDL 
Sbjct: 427 HNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLT 486

Query: 488 PLGLTGKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADF 547
           PLGLTGK YEKVCE+C IT+NK+ IF +NG+I PGGVRIG+PAMTSRGCLE +FE +ADF
Sbjct: 487 PLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADF 546

Query: 548 LHTAAQIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFE 588
           L+ AAQIA + QREHGKL K  LK +    E+ +LRN+VE+F+ QFAMP F+
Sbjct: 547 LYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

[Close]

BLAST of Sgr020681 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 1.2e-178
Identity = 298/464 (64.22%), Postives = 362/464 (78.02%), Query Frame = 0


Query: 123 VRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN +L   DP +  ++EKE++RQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY GN++IDEIE LCR RAL AF  D  +WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 303 ILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           +LICGGS+YPR+WDYARFR IADK GA+L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 423 LAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLTGK 482
           LAVALKQ +TP +K Y +QVK NA AL + L+ K +++VT GT+NH++LWDLRPLGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 483 NYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAAQI 542
             EK+C++C ITLNK  +FGD+  + PGGVRIG PAMTSRG +E DFE I +FL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 543 AISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGF 587
            + +Q+ +GKL K + KGL +N ++ +L+  VE FSA + MPGF
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

[Close]

BLAST of Sgr020681 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 9.4e-176
Identity = 291/464 (62.72%), Postives = 354/464 (76.29%), Query Frame = 0


Query: 123 VRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN +L   DP ++ ++EKE+ RQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY G ++IDEIE+LCR R+L AF  + + WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+LPYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 303 ILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           ++ICGG+SYPREWDYARFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E + YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 423 LAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLTGK 482
           LAVALKQV  P +K Y +QVK NA  LAS L+ K + LVT GTDNH++LWDLRPLGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 483 NYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAAQI 542
             EKVCE+C ITLN+  +FGD   + PGGVRIGTPAMTSRG +E DFE I +FLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 543 AISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGF 587
            + +Q ++GK+ K + KGL +N E+ E++  VE F+  F MPGF
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGF 462

[Close]

BLAST of Sgr020681 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.7e-156
Identity = 268/459 (58.39%), Postives = 337/459 (73.42%), Query Frame = 0


Query: 127 GNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGAR 186
           GN  L+  D  +F +M +E+ RQFKG+ELIASEN+  RAVMEALGSH TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 187 YYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDRIMGL 246
           YY G + +DE+ETLC++RAL AF LD   WGVNVQPYS + ANFAVYT LL P DRIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 247 DTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPKILIC 306
           D PSGG+ +HGY T + +K+S +SIFFES+PY++    G IDY +LEE AL F+PK++I 
Sbjct: 126 DLPSGGHLTHGYQT-DKKKISASSIFFESMPYQIGAD-GLIDYQRLEENALLFKPKLIIS 185

Query: 307 GGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLR 366
           G S+YPREWDY R R IADK GA LMCDMA  SGLVAA+   SPF YCDVVTSTTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 367 GPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVA 426
           GPR GIIF+R+G +      +   G E ++YD E KINFAVFPSLQGGPH N IA +AVA
Sbjct: 246 GPRSGIIFFRRGKR------VDGNGKEIEEYDIESKINFAVFPSLQGGPHENVIAGVAVA 305

Query: 427 LKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLTGKNYEK 486
           LK+ D+ E+K Y  QVKKNA A+ +AL+ K ++LVT GTDNH++LWDLRP  LTG  +EK
Sbjct: 306 LKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEK 365

Query: 487 VCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAAQIAISV 546
             +I  IT+NK  + GD   I PGG+RIG+ A+TSRG  E+DFE IADFL     I++ +
Sbjct: 366 AADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEI 425

Query: 547 QREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPG 586
           Q   GK    ++  +  + E+++LR  VE FS++F +PG
Sbjct: 426 QGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

[Close]

BLAST of Sgr020681 vs. ExPASy TrEMBL
Match: A0A6J1D7A8 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111017683 PE=3 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 567/587 (96.59%), Postives = 577/587 (98.30%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLSN HSNLSLGFSSHASPPHRAQIADDS+LLHLDSTRREP YSSPPVPLQLMEPQTES
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HRDEDG+AEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKR+SCEFDM+ RR
Sbjct: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQ LRVADPNLFGIMEKE+ RQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGLDSD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+ TPEYKAYMQQVKKNAQALASALLRKK+RLVTGGTDNHMLLWDLRPLGLT
Sbjct: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKNYEKVCEICQITLNKITI+GDNGIIIPGGVRIGTPAMTSRGCLESDFE I DFLHTAA
Sbjct: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAISVQREHGKLPKAYLKGLQ+NNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of Sgr020681 vs. ExPASy TrEMBL
Match: A0A6J1FV71 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447145 PE=3 SV=1)

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 552/587 (94.04%), Postives = 570/587 (97.10%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLS  HSNLSLGFSSHASPPHRAQIADDS+LLHLDS+RRE +YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HR++ GDAER+DD+YDDREVEEFRILGHSMCLKRRRDGESS+SSGS KRISCEFD+ETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHSMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+Q LRVADPNLFGIMEKE+QRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQV TPEYKAYMQQVKKNAQALA+ALLRKK++LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKNYE VCEIC ITLNKITIFGDNGIIIPGGVR+GTPAMTSRGCLESDFE IADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAISVQRE+GKLPK YLKGL SNNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of Sgr020681 vs. ExPASy TrEMBL
Match: A0A6J1IXT5 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479489 PE=3 SV=1)

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 549/587 (93.53%), Postives = 567/587 (96.59%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLS  HSNLSLGFSSHASPPHRAQIADDS+LLHLDS+RRE +YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HR++ GDAER+DD+YDDREVEEFRILGH MCLKRRRDGESS+SS S KRISCEFD+ETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+Q LRVADPNLFGIMEKE+QRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQV TPEYKAYMQQVKKNAQALA+ALLRKK++LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKNYE VCEIC ITLNKITIFGDNGIIIPGGVR+GTPAMTSRGCLESDFE IADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAISVQRE+GKLPK YLKGL SNNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of Sgr020681 vs. ExPASy TrEMBL
Match: A0A6J1E9K3 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111430628 PE=3 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 532/588 (90.48%), Postives = 555/588 (94.39%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLSN HSNLSLG SSHASPPHR QIADDSILLHL+S+R+EP +SSPP+PLQLMEP TES
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HRDE GDAEREDD YDDR+VEEFRILGHSMCLKRRRD ESS SS S+KR SCE D+ETRR
Sbjct: 61  HRDEVGDAEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSLSSSSSKRNSCERDLETRR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQ LR  DPNLFGIMEKE+QRQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRATDPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQV T EYKAYMQQVKKNAQALASALLRK ++LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKN+E+VCE+C ITLNKITIFGDNGII PGGVRIGTPAMTSRGCLESDFE+I +FLH AA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFET 589
           QIAI VQRE+GKLPKA+L+GLQSNNEVVEL  RV SFSA+FAMPG ET
Sbjct: 541 QIAIVVQRENGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 588

[Close]

BLAST of Sgr020681 vs. ExPASy TrEMBL
Match: A0A6J1JAX6 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482831 PE=3 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 8.2e-308
Identity = 523/588 (88.95%), Postives = 548/588 (93.20%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFSSHASPPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTES 60
           MDLSN +SNLSLG SSHASPPHR QIADDSILLHL+S+R+EP +SSPP+PLQLMEP TES
Sbjct: 1   MDLSNSNSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRISCEFDMETRR 120
           HRDE G AERE    DDR+VEEF ILGHSMCLKRRRD ESS SS S KR SCE D+ETRR
Sbjct: 61  HRDEVGGAERE----DDRDVEEFHILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQ LR ADPNLFGIMEKE+QRQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRE DY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYRRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGS+PR  AMLLYQGNE++QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSRPRTIAMLLYQGNESNQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQV T EYKAYMQQVKKNAQALASALLRK ++LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAA 540
           GKN+E+VCE+C ITLNKITIFGDNGII PGGVRIGTPAMTSRGCLESDFE+I +FLH AA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFET 589
           QIA+ VQREHGKLPK +L+GLQS+NEVVEL  RV SFSA+FAMPG ET
Sbjct: 541 QIAVVVQREHGKLPKPFLRGLQSSNEVVELGYRVASFSAKFAMPGLET 584

[Close]

BLAST of Sgr020681 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 830.1 bits (2143), Expect = 1.1e-240
Identity = 413/591 (69.88%), Postives = 478/591 (80.88%), Query Frame = 0


Query: 1   MDLSNPHSNLSLGFS-SHAS----PPHRAQIADDSILLHLDSTRREPAYSSPPVPLQLME 60
           MDLS   +N  LGF  SHAS    P  RA IADDSI L +D + R    +  P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  PQTESHRDEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGE-SSSSSGSTKRISC-E 120
            + E     D   +      D +E E FRILGH MCLKR+RD     + S   KR S  +
Sbjct: 61  QKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGD 120

Query: 121 FDMETRRSAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSH 180
            D+E+RR+AVR+WG+Q + +ADP++  +MEKE+QRQ +GIELIASEN+VCRAVMEALGSH
Sbjct: 121 SDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSH 180

Query: 181 LTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVY 240
           LTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL+SD WGVNVQPYSCTSANFAVY
Sbjct: 181 LTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVY 240

Query: 241 TGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLE 300
           TGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYKVNPQTGYIDYDKLE
Sbjct: 241 TGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLE 300

Query: 301 ERALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGY 360
           ++ALD+RPKILICGGSSYPR+WD+AR RQIADKCGAVLMCDMA ISGLVA KEC +PF +
Sbjct: 301 DKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDH 360

Query: 361 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQG 420
           CD+VTSTTHK LRGPRGGIIFYR+G K RK+       + +  YD EEKINFAVFPSLQG
Sbjct: 361 CDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQG 420

Query: 421 GPHNNHIAALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWD 480
           GPHNNHIAALA+ALKQV TPEYKAY+QQ+KKNAQALA+ALLR+K RLVTGGTDNH+LLWD
Sbjct: 421 GPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWD 480

Query: 481 LRPLGLTGKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIA 540
           L P+GLTGK YEKVCE+C ITLNK  IFGDNG I PGGVRIGTPAMT+RGC+ESDFE +A
Sbjct: 481 LTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMA 540

Query: 541 DFLHTAAQIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMP 585
           DFL  AAQI  ++QREHGK  K ++K L +N ++ ELRNRVE+F+ Q+ MP
Sbjct: 541 DFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

[Close]

BLAST of Sgr020681 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 825.5 bits (2131), Expect = 2.8e-239
Identity = 408/592 (68.92%), Postives = 484/592 (81.76%), Query Frame = 0


Query: 8   SNLSLGF-SSHASP---PHRAQIADDSILLHLDSTRREPAYSSPPVPLQLMEPQTESHR- 67
           S+LSLGF SSHA P   P R  IADDSI L +DS+ R  +   PPVPLQL+E + +    
Sbjct: 7   SDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVTGS 66

Query: 68  -----DEDGDAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSS--SSGSTKRISCEFD 127
                +ED +   ++DD D RE E+F +LGH M LKR R G S S  SS   KR   +  
Sbjct: 67  CSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDSG 126

Query: 128 METRRSAVRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLT 187
           +E+RR+ VR+WGNQ++  ADP +   MEKE+QRQF+GIELIASEN+VCRAVMEALGSHLT
Sbjct: 127 IESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLT 186

Query: 188 NKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTG 247
           NKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSANFAV+TG
Sbjct: 187 NKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTG 246

Query: 248 LLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEER 307
           LL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYKV+P+TGYIDYDKLEE+
Sbjct: 247 LLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEK 306

Query: 308 ALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCD 367
           ALD+RPKILICGGSSYPR+W++ RFR IADKCGAVLM DMAQISGLVAAKE  +PF YCD
Sbjct: 307 ALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCD 366

Query: 368 VVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGP 427
           +VTSTTHKSLRGPRGGIIFY++G KP+K+++ L       QYDFEEKINF+VFPSLQGGP
Sbjct: 367 IVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGP 426

Query: 428 HNNHIAALAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLR 487
           HNNHIAALA+ALKQ  +PEYK YM+QVKKNA+ALASAL+ +K +L+TGGTDNH+LLWDL 
Sbjct: 427 HNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLT 486

Query: 488 PLGLTGKNYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADF 547
           PLGLTGK YEKVCE+C IT+NK+ IF +NG+I PGGVRIG+PAMTSRGCLE +FE +ADF
Sbjct: 487 PLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADF 546

Query: 548 LHTAAQIAISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGFE 588
           L+ AAQIA + QREHGKL K  LK +    E+ +LRN+VE+F+ QFAMP F+
Sbjct: 547 LYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

[Close]

BLAST of Sgr020681 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 627.9 bits (1618), Expect = 8.4e-180
Identity = 298/464 (64.22%), Postives = 362/464 (78.02%), Query Frame = 0


Query: 123 VRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN +L   DP +  ++EKE++RQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY GN++IDEIE LCR RAL AF  D  +WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 303 ILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           +LICGGS+YPR+WDYARFR IADK GA+L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 423 LAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLTGK 482
           LAVALKQ +TP +K Y +QVK NA AL + L+ K +++VT GT+NH++LWDLRPLGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 483 NYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAAQI 542
             EK+C++C ITLNK  +FGD+  + PGGVRIG PAMTSRG +E DFE I +FL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 543 AISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGF 587
            + +Q+ +GKL K + KGL +N ++ +L+  VE FSA + MPGF
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

[Close]

BLAST of Sgr020681 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 618.2 bits (1593), Expect = 6.7e-177
Identity = 291/464 (62.72%), Postives = 354/464 (76.29%), Query Frame = 0


Query: 123 VRSWGNQNLRVADPNLFGIMEKERQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN +L   DP ++ ++EKE+ RQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY G ++IDEIE+LCR R+L AF  + + WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+LPYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 303 ILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           ++ICGG+SYPREWDYARFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E + YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 423 LAVALKQVDTPEYKAYMQQVKKNAQALASALLRKKHRLVTGGTDNHMLLWDLRPLGLTGK 482
           LAVALKQV  P +K Y +QVK NA  LAS L+ K + LVT GTDNH++LWDLRPLGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 483 NYEKVCEICQITLNKITIFGDNGIIIPGGVRIGTPAMTSRGCLESDFEIIADFLHTAAQI 542
             EKVCE+C ITLN+  +FGD   + PGGVRIGTPAMTSRG +E DFE I +FLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 543 AISVQREHGKLPKAYLKGLQSNNEVVELRNRVESFSAQFAMPGF 587
            + +Q ++GK+ K + KGL +N E+ E++  VE F+  F MPGF
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGF 462

[Close]

BLAST of Sgr020681 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 493.4 bits (1269), Expect = 2.5e-139
Identity = 252/503 (50.10%), Postives = 337/503 (67.00%), Query Frame = 0


Query: 89  SMCLKRRRDGESSSSSGSTKRISCEFDMETRRSAVRSWGNQNLRVADPNLFGIMEKERQR 148
           S  LKR    E  +SS S   +    ++ ++      +G   L   DP +  I+ KE+ R
Sbjct: 48  SSFLKRNLVSEMRASSVSLPNV----EISSKEIPFEDYG---LGEVDPEVRTIITKEKDR 107

Query: 149 QFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAA 208
           QF+ +ELIASEN+  RAVMEA+GS LTNKYSEG+PG RYY GN+YID++ETLC+ RALAA
Sbjct: 108 QFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAA 167

Query: 209 FGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSG 268
           F LDS  WGVNVQP S + ANFAVYT +L P DRIMGLD P GG+ SHG+ T   R+VSG
Sbjct: 168 FRLDSTKWGVNVQPLSGSPANFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAK-RRVSG 227

Query: 269 ASIFFESLPYKVNPQTGYIDYDKLEERALDFRPKILICGGSSYPREWDYARFRQIADKCG 328
            SI+FES+PY+++  TG +DYD LE+ A  FRPK++I G S+Y R++DY R R+IAD  G
Sbjct: 228 TSIYFESMPYRLDESTGIVDYDMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSVG 287

Query: 329 AVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLL 388
           A LM DMA ISGLVAA     PF YCD+VT+TTHKSLRGPRGG+IF+RK           
Sbjct: 288 AFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGGMIFFRK----------- 347

Query: 389 YQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVALKQVDTPEYKAYMQQVKKNAQA 448
              +  +  D E  +N AVFP LQGGPHN+ I  LAV LK   +PE+KAY ++V  N +A
Sbjct: 348 ---DPINGVDLESAVNNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKAYQKRVVSNCRA 407

Query: 449 LASALLRKKHRLVTGGTDNHMLLWDLRPLGLTGKNYEKVCEICQITLNKITIFGDNGIII 508
           LA+ L+    +LV+GG+DNH++L DLRP+G+ G   EK+ ++  ITLNK ++ GD   ++
Sbjct: 408 LANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMASITLNKNSVPGDKSALV 467

Query: 509 PGGVRIGTPAMTSRGCLESDFEIIADFLHTAAQIAISVQR-EHGKLPKAYLKGLQS---- 568
           PGG+RIG+PAMT+RG  E DF ++ADF+    +I +  ++   G   + + K + S    
Sbjct: 468 PGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAPGSKLQDFNKFVTSPEFP 527

Query: 569 -NNEVVELRNRVESFSAQFAMPG 586
               V  L+ RVE+F+++F +PG
Sbjct: 528 LKERVKSLKERVETFTSRFPIPG 528

The following BLAST results are available for this feature:

BLAST of Sgr020681 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149204.1	0.0e+00	96.59	serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149205.1 ser...	[more]
XP_022941930.1	0.0e+00	94.04	serine hydroxymethyltransferase 7-like [Cucurbita moschata] >KAG6600365.1 Serine...	[more]
XP_023547556.1	0.0e+00	93.70	serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo]	[more]
XP_022979958.1	0.0e+00	93.53	serine hydroxymethyltransferase 7-like [Cucurbita maxima]	[more]
KAG6576983.1	0.0e+00	90.65	Serine hydroxymethyltransferase 7, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Sgr020681 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WV0	1.6e-239	69.88	Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S...	[more]
Q9LM59	3.9e-238	68.92	Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S...	[more]
O23254	1.2e-178	64.22	Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S...	[more]
Q9SVM4	9.4e-176	62.72	Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S...	[more]
Q54Z26	1.7e-156	58.39	Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ...	[more]

BLAST of Sgr020681 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7A8	0.0e+00	96.59	Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111017683 P...	[more]
A0A6J1FV71	0.0e+00	94.04	Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447145 PE...	[more]
A0A6J1IXT5	0.0e+00	93.53	Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479489 PE=3...	[more]
A0A6J1E9K3	0.0e+00	90.48	Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111430628 PE...	[more]
A0A6J1JAX6	8.2e-308	88.95	Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482831 PE=3...	[more]

BLAST of Sgr020681 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36370.1	1.1e-240	69.88	serine hydroxymethyltransferase 7	[more]
AT1G22020.1	2.8e-239	68.92	serine hydroxymethyltransferase 6	[more]
AT4G13930.1	8.4e-180	64.22	serine hydroxymethyltransferase 4	[more]
AT4G13890.1	6.7e-177	62.72	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]
AT4G32520.1	2.5e-139	50.10	serine hydroxymethyltransferase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 160..440 e-value: 6.9E-194 score: 646.2
IPR039429	Serine hydroxymethyltransferase-like domain	PFAM	PF00464	SHMT	coord: 132..535 e-value: 5.5E-169 score: 562.0
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 135..584 e-value: 6.9E-194 score: 646.2
None	No IPR available	PIRSR	PIRSR000412-50	PIRSR000412-50	coord: 130..586 e-value: 9.7E-168 score: 556.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..18
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..77
None	No IPR available	PANTHER	PTHR11680:SF45	SERINE HYDROXYMETHYLTRANSFERASE	coord: 74..587
IPR001085	Serine hydroxymethyltransferase	PANTHER	PTHR11680	SERINE HYDROXYMETHYLTRANSFERASE	coord: 74..587
IPR001085	Serine hydroxymethyltransferase	HAMAP	MF_00051	SHMT	coord: 130..585 score: 39.605232
IPR001085	Serine hydroxymethyltransferase	CDD	cd00378	SHMT	coord: 135..577 e-value: 0.0 score: 574.15
IPR019798	Serine hydroxymethyltransferase, pyridoxal phosphate binding site	PROSITE	PS00096	SHMT	coord: 355..371
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 121..586

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr020681.1	Sgr020681.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046655	folic acid metabolic process
biological_process	GO:0019264	glycine biosynthetic process from serine
biological_process	GO:0006565	L-serine catabolic process
biological_process	GO:0032259	methylation
biological_process	GO:0035999	tetrahydrofolate interconversion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004372	glycine hydroxymethyltransferase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0070905	serine binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003824	catalytic activity