Sgr020644 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr020644
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	BUB1 N-terminal domain-containing protein
Location	tig00153552: 785263 .. 785715 (-)
RNA-Seq Expression	Sgr020644
Synteny	Sgr020644

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTTTGTGGATGATGAAGCAGGTGGTGAGATTGGCAACACAAGTTATGAAAAGATCATTCGAAATCGAGCAAATCACGAGATGTCTTGGGAGGTTCTACAGCATCTGCTTCACGTGCAAATGCGTTTGTGTTCCTTGTGTGGAGAATCTTTTGTCCGAAATGTTGATGATCCTGATGTACAAGATCCTATTCAAGCAAAGTTTAGAGAAGATACAGTTGTCTGTAGGTTTGTTGGTACTGCCATTTCAAATGAGCCAGAGGTGGAAAACATTTGCCACCATGGTTTAGTAGAGCCAACAGTTAACCTCAAAGAAGCCATGGATGATATCAACAACATGTTTGGAAAGCCCATTGAATTTGTAGGACTAGAAGATCCAAGAAGCCCGAAAGGCCACCAGAACTGCAAAATGATTTTGGTGGGTTCTCAATACTCCCTGATGATGAATTAG

mRNA sequence

ATGATTTTTGTGGATGATGAAGCAGGTGGTGAGATTGGCAACACAAGTTATGAAAAGATCATTCGAAATCGAGCAAATCACGAGATGTCTTGGGAGGTTCTACAGCATCTGCTTCACGTGCAAATGCGTTTGTGTTCCTTGTGTGGAGAATCTTTTGTCCGAAATGTTGATGATCCTGATGTACAAGATCCTATTCAAGCAAAGTTTAGAGAAGATACAGTTGTCTGTAGGTTTGTTGGTACTGCCATTTCAAATGAGCCAGAGGTGGAAAACATTTGCCACCATGGTTTAGTAGAGCCAACAGTTAACCTCAAAGAAGCCATGGATGATATCAACAACATGTTTGGAAAGCCCATTGAATTTGTAGGACTAGAAGATCCAAGAAGCCCGAAAGGCCACCAGAACTGCAAAATGATTTTGGTGGGTTCTCAATACTCCCTGATGATGAATTAG

Coding sequence (CDS)

ATGATTTTTGTGGATGATGAAGCAGGTGGTGAGATTGGCAACACAAGTTATGAAAAGATCATTCGAAATCGAGCAAATCACGAGATGTCTTGGGAGGTTCTACAGCATCTGCTTCACGTGCAAATGCGTTTGTGTTCCTTGTGTGGAGAATCTTTTGTCCGAAATGTTGATGATCCTGATGTACAAGATCCTATTCAAGCAAAGTTTAGAGAAGATACAGTTGTCTGTAGGTTTGTTGGTACTGCCATTTCAAATGAGCCAGAGGTGGAAAACATTTGCCACCATGGTTTAGTAGAGCCAACAGTTAACCTCAAAGAAGCCATGGATGATATCAACAACATGTTTGGAAAGCCCATTGAATTTGTAGGACTAGAAGATCCAAGAAGCCCGAAAGGCCACCAGAACTGCAAAATGATTTTGGTGGGTTCTCAATACTCCCTGATGATGAATTAG

Protein sequence

MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSLCGESFVRNVDDPDVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVGLEDPRSPKGHQNCKMILVGSQYSLMMN

Homology

[Close]

BLAST of Sgr020644 vs. NCBI nr
Match: XP_022136432.1 (probable inactive serine/threonine-protein kinase bub1 [Momordica charantia])

HSP 1 Score: 164.9 bits (416), Expect = 5.5e-37
Identity = 86/125 (68.80%), Postives = 98/125 (78.40%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSL---CGESFVRNVD 60
           MIFVDDEAGGEIGNTSYEK   + +    S + +        R  +    C E+ + +VD
Sbjct: 350 MIFVDDEAGGEIGNTSYEK---DGSKSSESRDFVGGSTTSASRANAFVFPCVEN-LEDVD 409

Query: 61  DPDVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGK 120
           DPD+QDPIQAKFREDT+VCRFVGTAIS+EPEVENICHHGLVEPTVNLKEAMDDINNMFG+
Sbjct: 410 DPDIQDPIQAKFREDTIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGE 469

Query: 121 PIEFV 123
           PIEFV
Sbjct: 470 PIEFV 470

[Close]

BLAST of Sgr020644 vs. NCBI nr
Match: XP_022984556.1 (uncharacterized protein LOC111482816 [Cucurbita maxima])

HSP 1 Score: 162.5 bits (410), Expect = 2.7e-36
Identity = 92/145 (63.45%), Postives = 100/145 (68.97%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSLCG-ESFV------ 60
           MIFVDDEAGGE+GNTSYEK            ++ +    V     S  G ++FV      
Sbjct: 319 MIFVDDEAGGELGNTSYEK---------DGSKLSESRYVVGSSTASASGSKAFVFPCVYN 378

Query: 61  ---RNVDDPDVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDD 120
               N DD DVQDPI+AKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDD
Sbjct: 379 PENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDD 438

Query: 121 INNMFGKPIEFVGLEDPRSPKGHQN 136
           INNMFGKPIEFV     R  K H+N
Sbjct: 439 INNMFGKPIEFV---RSRRSKKHEN 451

[Close]

BLAST of Sgr020644 vs. NCBI nr
Match: XP_023552017.1 (uncharacterized protein LOC111809814 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 162.5 bits (410), Expect = 2.7e-36
Identity = 92/144 (63.89%), Postives = 101/144 (70.14%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSLCGESFV------- 60
           MIFVDDEAGGE+GNTSYEK               ++++   M   S   ++FV       
Sbjct: 323 MIFVDDEAGGELGNTSYEK-------DGSKLSESRYVVGSSMASASR-SKAFVFPCVDNP 382

Query: 61  RNV--DDPDVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDI 120
            NV  DD DVQDPI+AKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDI
Sbjct: 383 ENVDDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDI 442

Query: 121 NNMFGKPIEFVGLEDPRSPKGHQN 136
           NNMFGKPIEFV     R  K H+N
Sbjct: 443 NNMFGKPIEFV---RSRRSKKHEN 455

[Close]

BLAST of Sgr020644 vs. NCBI nr
Match: KAG7015406.1 (Mitotic spindle checkpoint protein BUBR1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 161.4 bits (407), Expect = 6.1e-36
Identity = 86/136 (63.24%), Postives = 95/136 (69.85%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKI-IRNRANHEMSWEVLQHLLHVQMRLCSLCGESFVRNVDDP 60
           MIF+DDE GGE+GNTSYEK   +   +  +         H +  +          N DD 
Sbjct: 321 MIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASHSKAFIFPCVDNPENVNDDDS 380

Query: 61  DVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPI 120
           DVQDPI+AKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPI
Sbjct: 381 DVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPI 440

Query: 121 EFVGLEDPRSPKGHQN 136
           EFV     R  K H+N
Sbjct: 441 EFV---RSRRSKKHEN 453

[Close]

BLAST of Sgr020644 vs. NCBI nr
Match: XP_022929208.1 (uncharacterized protein LOC111435869 [Cucurbita moschata])

HSP 1 Score: 160.6 bits (405), Expect = 1.0e-35
Identity = 87/136 (63.97%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSLCGESFVR-NVDDP 60
           MIFVDDE GGE+GNTSYEK     ++                     C ++    N DD 
Sbjct: 323 MIFVDDEVGGELGNTSYEKDGSKLSDSRYVVGSSTASASRSKAFVFPCVDNPENVNDDDS 382

Query: 61  DVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPI 120
           DVQDPI+AKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPI
Sbjct: 383 DVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPI 442

Query: 121 EFVGLEDPRSPKGHQN 136
           EFV     R  K H+N
Sbjct: 443 EFV---RSRRSKKHEN 455

[Close]

BLAST of Sgr020644 vs. ExPASy TrEMBL
Match: A0A6J1C5H4 (probable inactive serine/threonine-protein kinase bub1 OS=Momordica charantia OX=3673 GN=LOC111008141 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 2.7e-37
Identity = 86/125 (68.80%), Postives = 98/125 (78.40%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSL---CGESFVRNVD 60
           MIFVDDEAGGEIGNTSYEK   + +    S + +        R  +    C E+ + +VD
Sbjct: 350 MIFVDDEAGGEIGNTSYEK---DGSKSSESRDFVGGSTTSASRANAFVFPCVEN-LEDVD 409

Query: 61  DPDVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGK 120
           DPD+QDPIQAKFREDT+VCRFVGTAIS+EPEVENICHHGLVEPTVNLKEAMDDINNMFG+
Sbjct: 410 DPDIQDPIQAKFREDTIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGE 469

Query: 121 PIEFV 123
           PIEFV
Sbjct: 470 PIEFV 470

[Close]

BLAST of Sgr020644 vs. ExPASy TrEMBL
Match: A0A6J1J5L0 (uncharacterized protein LOC111482816 OS=Cucurbita maxima OX=3661 GN=LOC111482816 PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.3e-36
Identity = 92/145 (63.45%), Postives = 100/145 (68.97%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSLCG-ESFV------ 60
           MIFVDDEAGGE+GNTSYEK            ++ +    V     S  G ++FV      
Sbjct: 319 MIFVDDEAGGELGNTSYEK---------DGSKLSESRYVVGSSTASASGSKAFVFPCVYN 378

Query: 61  ---RNVDDPDVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDD 120
               N DD DVQDPI+AKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDD
Sbjct: 379 PENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDD 438

Query: 121 INNMFGKPIEFVGLEDPRSPKGHQN 136
           INNMFGKPIEFV     R  K H+N
Sbjct: 439 INNMFGKPIEFV---RSRRSKKHEN 451

[Close]

BLAST of Sgr020644 vs. ExPASy TrEMBL
Match: A0A6J1ERG0 (uncharacterized protein LOC111435869 OS=Cucurbita moschata OX=3662 GN=LOC111435869 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 5.0e-36
Identity = 87/136 (63.97%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSLCGESFVR-NVDDP 60
           MIFVDDE GGE+GNTSYEK     ++                     C ++    N DD 
Sbjct: 323 MIFVDDEVGGELGNTSYEKDGSKLSDSRYVVGSSTASASRSKAFVFPCVDNPENVNDDDS 382

Query: 61  DVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPI 120
           DVQDPI+AKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPI
Sbjct: 383 DVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPI 442

Query: 121 EFVGLEDPRSPKGHQN 136
           EFV     R  K H+N
Sbjct: 443 EFV---RSRRSKKHEN 455

[Close]

BLAST of Sgr020644 vs. ExPASy TrEMBL
Match: A0A1S3CCD9 (uncharacterized protein LOC103499203 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499203 PE=4 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.9e-35
Identity = 83/125 (66.40%), Postives = 92/125 (73.60%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSL---CGESFVRNVD 60
           MIFVDDE+GGEIGNTS EK+         S +V+        R  +    C ++     D
Sbjct: 343 MIFVDDESGGEIGNTSIEKV---GLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENMDD 402

Query: 61  DPDVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGK 120
           D D QDP+QAKFREDT+VCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGK
Sbjct: 403 DSDAQDPMQAKFREDTIVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGK 462

Query: 121 PIEFV 123
           PIEFV
Sbjct: 463 PIEFV 464

[Close]

BLAST of Sgr020644 vs. ExPASy TrEMBL
Match: A0A5D3D6J0 (Mad3_BUB1_I domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00980 PE=4 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.9e-35
Identity = 83/125 (66.40%), Postives = 92/125 (73.60%), Query Frame = 0


Query: 1   MIFVDDEAGGEIGNTSYEKIIRNRANHEMSWEVLQHLLHVQMRLCSL---CGESFVRNVD 60
           MIFVDDE+GGEIGNTS EK+         S +V+        R  +    C ++     D
Sbjct: 343 MIFVDDESGGEIGNTSIEKV---GLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENMDD 402

Query: 61  DPDVQDPIQAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGK 120
           D D QDP+QAKFREDT+VCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGK
Sbjct: 403 DSDAQDPMQAKFREDTIVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGK 462

Query: 121 PIEFV 123
           PIEFV
Sbjct: 463 PIEFV 464

[Close]

BLAST of Sgr020644 vs. TAIR 10
Match: AT5G05510.1 (Mad3/BUB1 homology region 1 )

HSP 1 Score: 101.3 bits (251), Expect = 7.0e-22
Identity = 48/67 (71.64%), Postives = 57/67 (85.07%), Query Frame = 0


Query: 57  DDPDVQDPIQAKFREDTVVCRFVGTAISNEPE-VENICHHGLVEPTVNLKEAMDDINNMF 116
           ++ D   P +A+FREDTVV RFVG+ IS+EPE VEN CHHGLV+PTVNLKEAM+DINNMF
Sbjct: 324 EEADRNTPPRARFREDTVVRRFVGSTISDEPEAVENACHHGLVDPTVNLKEAMEDINNMF 383

Query: 117 GKPIEFV 123
           G+PI FV
Sbjct: 384 GEPINFV 390

The following BLAST results are available for this feature:

BLAST of Sgr020644 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136432.1	5.5e-37	68.80	probable inactive serine/threonine-protein kinase bub1 [Momordica charantia]	[more]
XP_022984556.1	2.7e-36	63.45	uncharacterized protein LOC111482816 [Cucurbita maxima]	[more]
XP_023552017.1	2.7e-36	63.89	uncharacterized protein LOC111809814 [Cucurbita pepo subsp. pepo]	[more]
KAG7015406.1	6.1e-36	63.24	Mitotic spindle checkpoint protein BUBR1 [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022929208.1	1.0e-35	63.97	uncharacterized protein LOC111435869 [Cucurbita moschata]	[more]

BLAST of Sgr020644 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Sgr020644 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5H4	2.7e-37	68.80	probable inactive serine/threonine-protein kinase bub1 OS=Momordica charantia OX...	[more]
A0A6J1J5L0	1.3e-36	63.45	uncharacterized protein LOC111482816 OS=Cucurbita maxima OX=3661 GN=LOC111482816...	[more]
A0A6J1ERG0	5.0e-36	63.97	uncharacterized protein LOC111435869 OS=Cucurbita moschata OX=3662 GN=LOC1114358...	[more]
A0A1S3CCD9	1.9e-35	66.40	uncharacterized protein LOC103499203 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5D3D6J0	1.9e-35	66.40	Mad3_BUB1_I domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of Sgr020644 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05510.1	7.0e-22	71.64	Mad3/BUB1 homology region 1	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015661	Mitotic spindle checkpoint protein Bub1/Mad3	PANTHER	PTHR14030	MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1	coord: 2..129
None	No IPR available	PANTHER	PTHR14030:SF2	OS11G0128700 PROTEIN	coord: 2..129

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr020644.1	Sgr020644.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051754	meiotic sister chromatid cohesion, centromeric
biological_process	GO:0007094	mitotic spindle assembly checkpoint signaling
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0004672	protein kinase activity