Sgr020619 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr020619 vs. NCBI nr
Match: XP_022985135.1 (uncharacterized protein LOC111483218, partial [Cucurbita maxima])

HSP 1 Score: 169.5 bits (428), Expect = 2.1e-38
Identity = 99/147 (67.35%), Postives = 108/147 (73.47%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQSSTSDSVSTVHSFSSSSSSIPPSS 60
           MGKSESAK CKRH NHN LPGICPSCLRERL Q  QSS   S++   S S+SSSS   SS
Sbjct: 56  MGKSESAKECKRHQNHNQLPGICPSCLRERLQQLQQSS---SINYSDSRSTSSSSFLKSS 115

Query: 61  ELFFSAESPRRRHHRRNASELVT---ASDFFGDKLKKSGSIGLDAGCYHGGGGGKKKKGG 120
           E FFS  +  RR H RNASELVT   A+D FGDK+KKS SI + A    GGGGG KKK G
Sbjct: 116 ESFFSGNTSWRRRHTRNASELVTGSMAADLFGDKVKKSSSIRISADGGGGGGGGGKKKVG 175

Query: 121 FWSKLLLRPKALYFHSQPKV-SREILG 144
           FWS+ L+RPKALYFHS PKV SREILG
Sbjct: 176 FWSRFLVRPKALYFHSHPKVSSREILG 199

BLAST of Sgr020619 vs. NCBI nr
Match: XP_023553119.1 (uncharacterized protein LOC111810620 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 164.1 bits (414), Expect = 9.0e-37
Identity = 99/147 (67.35%), Postives = 107/147 (72.79%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQSSTSDSVSTVHSFSSSSSSIPPSS 60
           MGKSESA+ CKRH NHN LPGICPSCLRERL Q  QSS S S     S S+SSSS   SS
Sbjct: 1   MGKSESARECKRHQNHNQLPGICPSCLRERLQQLQQSSISYS----DSRSTSSSSFLKSS 60

Query: 61  ELFFSAESPRRRHHRRNASELVT---ASDFFGDKLKKSGSIGLDAGCYHGGGGGKKKKGG 120
           E FFS  +  RR H RNASELVT   A+D FGDK+KKS SI + A    GGGGG KKK G
Sbjct: 61  ESFFSGNTSWRRRHTRNASELVTGSMAADLFGDKVKKSSSIRISAD-GGGGGGGGKKKVG 120

Query: 121 FWSKLLLRPKALYFHSQPKV-SREILG 144
           FWS+ L+RPKALYFHS PKV SREILG
Sbjct: 121 FWSRFLVRPKALYFHSHPKVSSREILG 142

BLAST of Sgr020619 vs. NCBI nr
Match: XP_022929275.1 (uncharacterized protein LOC111435907 [Cucurbita moschata])

HSP 1 Score: 160.6 bits (405), Expect = 9.9e-36
Identity = 96/147 (65.31%), Postives = 104/147 (70.75%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQSSTSDSVSTVHSFSSSSSSIPPSS 60
           MGKSESAK CKRH NHN LPGICPSCLRERL Q  QS    S+S   S S+SSSS   SS
Sbjct: 1   MGKSESAKECKRHQNHNQLPGICPSCLRERLQQLQQS----SISYADSRSTSSSSFLKSS 60

Query: 61  ELFFSAESPRRRHHRRNASELVT---ASDFFGDKLKKSGSIGLDAGCYHGGGGGKKKKGG 120
           E FFS  +  RR H RNASEL T   A+D FGDK+KKS SI + A      GGG KKK G
Sbjct: 61  ESFFSGNTSWRRRHTRNASELFTGSMAADLFGDKVKKSSSIRISA-----DGGGGKKKVG 120

Query: 121 FWSKLLLRPKALYFHSQPKV-SREILG 144
           FWS+ L+RPKALYFHS PKV SREILG
Sbjct: 121 FWSRFLVRPKALYFHSHPKVSSREILG 138

BLAST of Sgr020619 vs. NCBI nr
Match: XP_022989609.1 (uncharacterized protein LOC111486643 [Cucurbita maxima])

HSP 1 Score: 157.5 bits (397), Expect = 8.4e-35
Identity = 95/151 (62.91%), Postives = 106/151 (70.20%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQSST--SDSVSTVHSFSSSSSSIPP 60
           MGKSESAK CKRHPNH LLPGICPSCLRE L +F+  S+  SDS ST   FSSSSSS P 
Sbjct: 1   MGKSESAKECKRHPNHRLLPGICPSCLRESLQRFNNQSSIYSDSAST---FSSSSSSFPH 60

Query: 61  SSELFFSAESPR--RRHHRRNASELV---TASDFFGDKLKKSGSIGLDAGCYHGGGGGKK 120
           SSE FFS +SPR  R HH+RNASE+V    A+D FGDKLK SG              G K
Sbjct: 61  SSEFFFSGDSPRRTRHHHKRNASEMVMRSRAADLFGDKLKMSGE-------------GGK 120

Query: 121 KKGGFWSKLLLRPKALYFHSQPKVS-REILG 144
           KKGGFWS+L+   KA +FHSQPKVS REI+G
Sbjct: 121 KKGGFWSRLMGGRKAFHFHSQPKVSCREIIG 135

BLAST of Sgr020619 vs. NCBI nr
Match: XP_023544931.1 (uncharacterized protein LOC111804380 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 155.6 bits (392), Expect = 3.2e-34
Identity = 94/151 (62.25%), Postives = 106/151 (70.20%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQSST--SDSVSTVHSFSSSSSSIPP 60
           MGKSESAK CKRHPNH LLPGICPSCLRE L +F+  S+  SDSVST   FSSSSSS P 
Sbjct: 1   MGKSESAKDCKRHPNHRLLPGICPSCLRESLQRFNNQSSIYSDSVST---FSSSSSSFPH 60

Query: 61  SSELFFSAESPR--RRHHRRNASELV---TASDFFGDKLKKSGSIGLDAGCYHGGGGGKK 120
           SSE FFS +SPR  R HH+RNASE+V    A+D FGDK+K              GG G K
Sbjct: 61  SSEFFFSGDSPRRTRHHHKRNASEMVMRSRAADLFGDKVKM-------------GGEGGK 120

Query: 121 KKGGFWSKLLLRPKALYFHSQPKV-SREILG 144
           KKGGFWS+L+   KA +FHSQPKV  REI+G
Sbjct: 121 KKGGFWSRLMGGRKAFHFHSQPKVCCREIMG 135

BLAST of Sgr020619 vs. ExPASy TrEMBL
Match: A0A6J1JAI6 (uncharacterized protein LOC111483218 OS=Cucurbita maxima OX=3661 GN=LOC111483218 PE=4 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 1.0e-38
Identity = 99/147 (67.35%), Postives = 108/147 (73.47%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQSSTSDSVSTVHSFSSSSSSIPPSS 60
           MGKSESAK CKRH NHN LPGICPSCLRERL Q  QSS   S++   S S+SSSS   SS
Sbjct: 56  MGKSESAKECKRHQNHNQLPGICPSCLRERLQQLQQSS---SINYSDSRSTSSSSFLKSS 115

Query: 61  ELFFSAESPRRRHHRRNASELVT---ASDFFGDKLKKSGSIGLDAGCYHGGGGGKKKKGG 120
           E FFS  +  RR H RNASELVT   A+D FGDK+KKS SI + A    GGGGG KKK G
Sbjct: 116 ESFFSGNTSWRRRHTRNASELVTGSMAADLFGDKVKKSSSIRISADGGGGGGGGGKKKVG 175

Query: 121 FWSKLLLRPKALYFHSQPKV-SREILG 144
           FWS+ L+RPKALYFHS PKV SREILG
Sbjct: 176 FWSRFLVRPKALYFHSHPKVSSREILG 199

BLAST of Sgr020619 vs. ExPASy TrEMBL
Match: A0A6J1ETY8 (uncharacterized protein LOC111435907 OS=Cucurbita moschata OX=3662 GN=LOC111435907 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.8e-36
Identity = 96/147 (65.31%), Postives = 104/147 (70.75%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQSSTSDSVSTVHSFSSSSSSIPPSS 60
           MGKSESAK CKRH NHN LPGICPSCLRERL Q  QS    S+S   S S+SSSS   SS
Sbjct: 1   MGKSESAKECKRHQNHNQLPGICPSCLRERLQQLQQS----SISYADSRSTSSSSFLKSS 60

Query: 61  ELFFSAESPRRRHHRRNASELVT---ASDFFGDKLKKSGSIGLDAGCYHGGGGGKKKKGG 120
           E FFS  +  RR H RNASEL T   A+D FGDK+KKS SI + A      GGG KKK G
Sbjct: 61  ESFFSGNTSWRRRHTRNASELFTGSMAADLFGDKVKKSSSIRISA-----DGGGGKKKVG 120

Query: 121 FWSKLLLRPKALYFHSQPKV-SREILG 144
           FWS+ L+RPKALYFHS PKV SREILG
Sbjct: 121 FWSRFLVRPKALYFHSHPKVSSREILG 138

BLAST of Sgr020619 vs. ExPASy TrEMBL
Match: A0A6J1JPT9 (uncharacterized protein LOC111486643 OS=Cucurbita maxima OX=3661 GN=LOC111486643 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.1e-35
Identity = 95/151 (62.91%), Postives = 106/151 (70.20%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQSST--SDSVSTVHSFSSSSSSIPP 60
           MGKSESAK CKRHPNH LLPGICPSCLRE L +F+  S+  SDS ST   FSSSSSS P 
Sbjct: 1   MGKSESAKECKRHPNHRLLPGICPSCLRESLQRFNNQSSIYSDSAST---FSSSSSSFPH 60

Query: 61  SSELFFSAESPR--RRHHRRNASELV---TASDFFGDKLKKSGSIGLDAGCYHGGGGGKK 120
           SSE FFS +SPR  R HH+RNASE+V    A+D FGDKLK SG              G K
Sbjct: 61  SSEFFFSGDSPRRTRHHHKRNASEMVMRSRAADLFGDKLKMSGE-------------GGK 120

Query: 121 KKGGFWSKLLLRPKALYFHSQPKVS-REILG 144
           KKGGFWS+L+   KA +FHSQPKVS REI+G
Sbjct: 121 KKGGFWSRLMGGRKAFHFHSQPKVSCREIIG 135

BLAST of Sgr020619 vs. ExPASy TrEMBL
Match: A0A0A0KZB2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G026920 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.3e-33
Identity = 94/137 (68.61%), Postives = 101/137 (73.72%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQS-STSDSVSTVHSFSSSSSSIPPS 60
           MGKSESAK CKRHPNHNLLPGICPSCLRE+L QFHQS + SDS ST   FSS SSS   S
Sbjct: 1   MGKSESAKECKRHPNHNLLPGICPSCLREKLQQFHQSPNYSDSQST---FSSPSSS---S 60

Query: 61  SELFFSAESPR--RRHHRRNASELVTAS---DFFGDKLKKSGSIGLDAGCYHGGGGGKKK 120
           S+ FFSA+S R  RRHHRRNASE VT S   D   DKLKK+GSI +      G G   KK
Sbjct: 61  SDFFFSADSSRRHRRHHRRNASEFVTGSMAVDLLADKLKKTGSIRISTDGGTGAGVKGKK 120

Query: 121 KGGFWSKLLLRPKALYF 132
           K GFWS+LLLRPKALYF
Sbjct: 121 KLGFWSRLLLRPKALYF 131

BLAST of Sgr020619 vs. ExPASy TrEMBL
Match: A0A5D3CZT1 (DUF740 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00010 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 3.8e-33
Identity = 94/138 (68.12%), Postives = 101/138 (73.19%), Query Frame = 0

Query: 1   MGKSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQS-STSDSVSTVHSFSSSSSSIPPS 60
           MGKSESAK CKRHPN+NLLPGICPSCLRE+L QFHQS + SDS ST   FSS SSS   S
Sbjct: 1   MGKSESAKECKRHPNYNLLPGICPSCLREKLQQFHQSPNYSDSQST---FSSPSSS---S 60

Query: 61  SELFFSAESP---RRRHHRRNASELVTAS---DFFGDKLKKSGSIGLDAGCYHGGGGGKK 120
           S+ FFSA+S    RR HHRRNASE VT S   D   DKLKKSGSI + A    G G   K
Sbjct: 61  SDFFFSADSSRRHRRHHHRRNASEFVTGSMAVDLLADKLKKSGSIRISADGSTGAGVKGK 120

Query: 121 KKGGFWSKLLLRPKALYF 132
           KK GFWS+LLLRPKALYF
Sbjct: 121 KKLGFWSRLLLRPKALYF 132

BLAST of Sgr020619 vs. TAIR 10
Match: AT1G35210.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 9 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF740 (InterPro:IPR008004); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G22470.1); Has 83 Blast hits to 83 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 81; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 41.2 bits (95), Expect = 8.2e-04
Identity = 21/53 (39.62%), Postives = 33/53 (62.26%), Query Frame = 0

Query: 3  KSESAKGCKRHPNHNLLPGICPSCLRERLLQFHQSSTSDSVSTVHSFSSSSSS 56
          K+++A  CK+HP H   PG+C  CL ERL  F ++++S    +    S+SSS+
Sbjct: 24 KTKNAVFCKKHPKHRQSPGVCSLCLNERLSLFIKAASSRRPRSRQILSTSSST 76