Sgr020593 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr020593 vs. NCBI nr
Match: XP_022136552.1 (vam6/Vps39-like protein [Momordica charantia])

HSP 1 Score: 1739.5 bits (4504), Expect = 0.0e+00
Identity = 890/939 (94.78%), Postives = 911/939 (97.02%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFELLKDNPSKIE+I SYGSKLFIGCSDGSLRIYSPE+SG DRSPPSEFHSKS
Sbjct: 1   MVHSAYDSFELLKDNPSKIEAIVSYGSKLFIGCSDGSLRIYSPESSGSDRSPPSEFHSKS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
           MELQKEPYVLE+NVTGFSRRSL+SMEV+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  MELQKEPYVLEKNVTGFSRRSLLSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPD VKSMSWCGENICLGI+R
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDIVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSGALTDVFP GRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGALTDVFPPGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPSV+VIQKPYA+ALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHS HALVVGL NSAY
Sbjct: 241 EAPSVVVIQKPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSNHALVVGLGNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQ TASGNFEEALALCKLLPPEDS+LRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQFTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPSIVLPKTTLVTETEKLMD+TLDGPHLSRGSSGFSDDMES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLVTETEKLMDLTLDGPHLSRGSSGFSDDMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           PPHQL+ESDEN TLESKKMNHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PPHQLVESDENATLESKKMNHNTLMALIKFLQKKRHSIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQS AALELLKGPNYCD+KICEEILQK
Sbjct: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSLAALELLKGPNYCDVKICEEILQK 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           +N+YSALLELYKCNSMHREALKLLHQLVEESKANES  ELIQKF PEMIIDYLKPLCGTD
Sbjct: 541 NNHYSALLELYKCNSMHREALKLLHQLVEESKANES--ELIQKFNPEMIIDYLKPLCGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSM VLESCPTQTIELFLSGNIPADLVN YLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMIVLESCPTQTIELFLSGNIPADLVNFYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEK YSSTRKKLLSALD ISGYHPEVLLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKIYSSTRKKLLSALDSISGYHPEVLLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           PSDALYEERAILLGKMNQHELALSLYVHKIHV E ALSYCDRVYESVAYQQSTKSSGNIY
Sbjct: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVSELALSYCDRVYESVAYQQSTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           LTLLQIYLNPRRTTKNFEKRITNLTSPQ MGTPKLGSGP+FKVKGGRAA+KIAAIEGAED
Sbjct: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQTMGTPKLGSGPTFKVKGGRAARKIAAIEGAED 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
           M+IS SNTDSGRSDGDTDE GEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 MKISHSNTDSGRSDGDTDEMGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSY 940
           NL QFLGPLLRKSSEAYRNS VIKSLRQSENLQ +++ Y
Sbjct: 901 NLRQFLGPLLRKSSEAYRNSLVIKSLRQSENLQVRDELY 937

BLAST of Sgr020593 vs. NCBI nr
Match: XP_022984182.1 (vam6/Vps39-like protein [Cucurbita maxima])

HSP 1 Score: 1704.1 bits (4412), Expect = 0.0e+00
Identity = 873/939 (92.97%), Postives = 904/939 (96.27%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP++SG DRSP S+FHS S
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPKSSGSDRSPTSDFHSMS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
           MELQKEPYVLE+NVTGFSRRSLVSM+V+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  MELQKEPYVLEKNVTGFSRRSLVSMKVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR
Sbjct: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSGALT+VFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGALTNVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPSV+VIQKPYAIALLPRYIEIRSL SPYALIQTIVLRNGRHLI S HALVVGLDNSAY
Sbjct: 241 EAPSVVVIQKPYAIALLPRYIEIRSLWSPYALIQTIVLRNGRHLIDSNHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDS+LRSAKESSIHIRYAH LFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHNLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPSI LPKTTL+TETEKLMDMTLD PHLS GSSGFSD+MES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIALPKTTLITETEKLMDMTLDDPHLSGGSSGFSDEMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           PPHQL+ESD NT+LESKK+NHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PPHQLLESDGNTSLESKKVNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKG NYCD+KICEEILQK
Sbjct: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           + +YSALLELY+CNSMHREALKLLHQLVEESK +ESQ EL QKFKPEMIIDYLK +CGTD
Sbjct: 541 NKHYSALLELYRCNSMHREALKLLHQLVEESKTSESQTELTQKFKPEMIIDYLKTICGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVLDW+ADLSAQHKWDEKTYS TRKKLLSAL+ ISGYHPE+LLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSPTRKKLLSALETISGYHPEILLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           P DAL EERAILLGKMNQHELALSLYVHKIH PE ALSYCDRVYESVA QQSTKSSGNIY
Sbjct: 721 PPDALSEERAILLGKMNQHELALSLYVHKIHAPELALSYCDRVYESVANQQSTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           LTLLQIYLNPRRTTK FEKRITNLTSPQN GTPKLGSGPS KVKG RA+KKIAAIEGAED
Sbjct: 781 LTLLQIYLNPRRTTKYFEKRITNLTSPQNTGTPKLGSGPSSKVKGSRASKKIAAIEGAED 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            +ISLSNT+S RSDGDTDETGEEGSSSIMLDEA+DLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 TKISLSNTESSRSDGDTDETGEEGSSSIMLDEAMDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSY 940
           NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQ +++ Y
Sbjct: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELY 939

BLAST of Sgr020593 vs. NCBI nr
Match: XP_038895209.1 (vacuolar sorting protein 39 [Benincasa hispida])

HSP 1 Score: 1704.1 bits (4412), Expect = 0.0e+00
Identity = 877/939 (93.40%), Postives = 904/939 (96.27%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSP + G DRSP S+FHSKS
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSFGSDRSPSSDFHSKS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            ELQKEPYVLE+NV+GFSRRSLVSMEV+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  TELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPS++VIQ PYA+ALLPRYIEIRSLRSPYALIQTIVLRNGRHLI SKHALVVGLDNSAY
Sbjct: 241 EAPSIVVIQNPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDS+LRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPSIVLPKTTL+TETEKLMD  L  PHLSRGSSGFSDDMES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLITETEKLMD--LGDPHLSRGSSGFSDDMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           PP+QL+ESDENT+LESKKMNHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PPNQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKG NYCD+KICEEILQK
Sbjct: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           + +YSALLELY+CNSMHREALKLLHQLVEESK NESQ E+IQKFKPEMIIDYLKPLCGTD
Sbjct: 541 NKHYSALLELYRCNSMHREALKLLHQLVEESKGNESQTEVIQKFKPEMIIDYLKPLCGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAP LQATYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPTLQATYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVLDW+ADLSAQ KWDEKTYSSTRKKLLSAL+ ISGY PEVLLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLDWYADLSAQCKWDEKTYSSTRKKLLSALESISGYQPEVLLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           PSDAL EERAILLGKMNQHELALSLYVHKIHVPE ALSYCDRVYES   QQ TKSSGNIY
Sbjct: 721 PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYES--NQQPTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKL SG SFKVKGGRAAKKIAAIEGAED
Sbjct: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLASGSSFKVKGGRAAKKIAAIEGAED 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
           M+ISL NTDS RSDGDTDE GEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 MKISLGNTDSSRSDGDTDEPGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSY 940
           NLLQF+GPLLRKSSEAYRNSSVIKSLRQSENLQ +++ Y
Sbjct: 901 NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELY 935

BLAST of Sgr020593 vs. NCBI nr
Match: XP_008453745.1 (PREDICTED: vam6/Vps39-like protein [Cucumis melo] >KAA0044743.1 vam6/Vps39-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1701.4 bits (4405), Expect = 0.0e+00
Identity = 875/939 (93.18%), Postives = 906/939 (96.49%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP +S  DRS  S+FH +S
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPNSSASDRSSSSDFHPRS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            ELQKEPYVLE+NV+GFSRRSLVSMEV+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  TELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPSV+VIQ PYA+ALLPRYIEIRSLRSPYALIQTIVLRNGRHLI SKHALVVGLDNSAY
Sbjct: 241 EAPSVVVIQNPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDS+LRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPSIVLPKTTLVTETEKL+D  LD PHLSRGSSGFSDDMES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLVTETEKLVD--LDDPHLSRGSSGFSDDMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           P HQL+ESDENT+LESKK+NHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PVHQLLESDENTSLESKKVNHNTLMALIKFLQKKRHSIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKG NYCD+KICEEILQK
Sbjct: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           + +YSALLELY+CNSMHREALKLLHQLVEESKANESQ EL QKFKPEMIIDYLKPLCGTD
Sbjct: 541 NKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTEL-QKFKPEMIIDYLKPLCGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVL+W+ADLSAQ+KWDEK YSSTRKKLLSAL+ ISGY PEVLLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLEWYADLSAQNKWDEKIYSSTRKKLLSALESISGYQPEVLLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           PSDAL EERAILLGKMNQHELALSLYVHKIHVPE ALSYCDRVYESVA QQ TKSSGNIY
Sbjct: 721 PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGR+AKKIAAIEGAED
Sbjct: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRSAKKIAAIEGAED 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            ++SLSNTDS RSDGDTDE GEEGSSSIMLDEAL+LLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 TKVSLSNTDSSRSDGDTDEPGEEGSSSIMLDEALNLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSY 940
           NLLQF+GPLLRKSSEAYRNSSVIKSLRQSENLQ +++ Y
Sbjct: 901 NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELY 936

BLAST of Sgr020593 vs. NCBI nr
Match: XP_022941902.1 (vam6/Vps39-like protein [Cucurbita moschata] >XP_022941903.1 vam6/Vps39-like protein [Cucurbita moschata])

HSP 1 Score: 1699.5 bits (4400), Expect = 0.0e+00
Identity = 874/996 (87.75%), Postives = 920/996 (92.37%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFEL KDNPSKIESIESYGSKLFIGCSDGSLRIYSP +SG DRS  S+FHS S
Sbjct: 1   MVHSAYDSFELFKDNPSKIESIESYGSKLFIGCSDGSLRIYSPVSSGPDRSLSSDFHSMS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
           MELQKEPY+LE+NV GFSRRSLVSM+V+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  MELQKEPYILEKNVKGFSRRSLVSMKVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121 ANTYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSG LTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGTLTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPSV+VIQ PYA+ALLPRYIE+RSLRSPY+LIQTIVLRNGRHLI SKHA VVGLDNSAY
Sbjct: 241 EAPSVVVIQNPYAVALLPRYIEVRSLRSPYSLIQTIVLRNGRHLIDSKHASVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDS LRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSILRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPS+VLPKTTL+TETEKLMDMTLD PHLSR SS FSDDMES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSLVLPKTTLITETEKLMDMTLDDPHLSRVSSDFSDDMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           PPHQL+ESDE++TLESKKMNHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PPHQLLESDEHSTLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYK R N+PISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQ+
Sbjct: 481 KSYKARANVPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQE 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           +N+YSALLELYKCNSMHREALK+LHQLVEESKAN+SQ E IQ FKPEMIIDYLK LCGTD
Sbjct: 541 NNHYSALLELYKCNSMHREALKILHQLVEESKANKSQPEPIQMFKPEMIIDYLKTLCGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSM +LESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQ TYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMPILESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQGTYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVL+W+ADL+AQHKWDEKTYSSTRKKLLSAL+ ISGYHPEVLLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLEWYADLTAQHKWDEKTYSSTRKKLLSALETISGYHPEVLLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           PSDALYEERAILLGK+NQHELALSLYVHKIHVPE ALSYCDRVYESVAYQQSTKSSGNIY
Sbjct: 721 PSDALYEERAILLGKLNQHELALSLYVHKIHVPELALSYCDRVYESVAYQQSTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           L LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPK+GSGPSFKVKGGRAAKKI AIEGAE+
Sbjct: 781 LILLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKIGSGPSFKVKGGRAAKKIVAIEGAEN 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            +ISLS TDSGR+DGDTDET EEGSS IMLDEALDLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 TKISLSKTDSGRTDGDTDETCEEGSSPIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSYTYTAIGCR---NCCCTV---- 960
           NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQ ++  Y       +   N  C++    
Sbjct: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQVRDDLYKQRKPEIKVTSNSVCSLCNKK 960

Query: 961 ---TAYRSFPTAVPLPELLLKKKSYLLLPTEASQPL 987
              + +  +PT + L   +  + S  +       P+
Sbjct: 961 IGTSVFAVYPTGITLVHFVCFRDSQSMKAVSKGSPI 996

BLAST of Sgr020593 vs. ExPASy Swiss-Prot
Match: Q8L5Y0 (Vacuolar sorting protein 39 OS=Arabidopsis thaliana OX=3702 GN=VPS39 PE=1 SV=1)

HSP 1 Score: 1321.2 bits (3418), Expect = 0.0e+00
Identity = 684/952 (71.85%), Postives = 803/952 (84.35%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVH+AYDSF+LLKD P++I+++ESYGSKLF GC DGSLRIYSP  S    S PSE H   
Sbjct: 1   MVHNAYDSFQLLKDCPARIDAVESYGSKLFAGCYDGSLRIYSPPESSA--SDPSELH--- 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
               +E YVLE+ V GFS++ +V+MEVL SRELLL+LSESIAFH LPNLET+AVITKAKG
Sbjct: 61  ----QETYVLEKTVAGFSKKPIVAMEVLASRELLLSLSESIAFHGLPNLETVAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCF+RQKRVC+F+HDGG GFVEV+++GVPDTVKS+SWCGENICLGI++
Sbjct: 121 ANAYSWDDRRGFLCFSRQKRVCVFKHDGGGGFVEVRDYGVPDTVKSISWCGENICLGIKK 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILN  +G L++VFPSGR+APPLV+SLPSGEL+LGK+NIGVFVDQNGKLLQ  RICWS
Sbjct: 181 EYVILNTANGTLSEVFPSGRVAPPLVISLPSGELILGKENIGVFVDQNGKLLQTERICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAP+ IVIQ PYAIALLPR +E+R LRSPY LIQTIVL+N R L+ S +A++VGLDNS Y
Sbjct: 241 EAPTSIVIQNPYAIALLPRRVEVRLLRSPYPLIQTIVLQNIRRLVKSNNAVIVGLDNSVY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
            LFPV +GAQIVQLTASGNFEEALALCK+LPP++S+LR+AKESSIH R+AHYLF+NGSYE
Sbjct: 301 VLFPVSIGAQIVQLTASGNFEEALALCKVLPPDESSLRAAKESSIHTRFAHYLFENGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDME- 420
           EAMEHF ASQVDIT+VL  YPSI+LPKTT++ + +K++D++ D   LSRGSSG SDDME 
Sbjct: 361 EAMEHFLASQVDITHVLSMYPSIILPKTTIIPQPDKMVDISGDEASLSRGSSGISDDMES 420

Query: 421 SPPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRY 480
           S P   +ES++N  LESKKM+HNTLMALIK+L K+R  +IEKAT+EGTEEV+ DAVG  Y
Sbjct: 421 SSPRYFLESEDNADLESKKMSHNTLMALIKYLLKRRPAVIEKATSEGTEEVISDAVGKTY 480

Query: 481 --------KKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDI 540
                   KKS KGRG IP++SGAREMAAILDTALLQALL TGQS AA+ELLKG NY D+
Sbjct: 481 GANDSSKSKKSSKGRGMIPLNSGAREMAAILDTALLQALLHTGQSGAAIELLKGVNYSDV 540

Query: 541 KICEEILQKSNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIID 600
           KICEEIL KS NYSALLEL+K NSMH EALKLL+QL +ESK N+SQ ++ Q F PE+II+
Sbjct: 541 KICEEILMKSKNYSALLELFKSNSMHHEALKLLNQLADESKTNQSQTDVTQIFSPELIIE 600

Query: 601 YLKPLCGTDPILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLEL 660
           YLKPLC TDP+LVLE+SM VLESCPTQTI+LFLSGNI ADLVNSYLKQHAPN+Q  YLEL
Sbjct: 601 YLKPLCRTDPMLVLEYSMLVLESCPTQTIDLFLSGNISADLVNSYLKQHAPNMQGRYLEL 660

Query: 661 MLAMNESSISGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGY 720
           M+AMN++++SGNLQNEM+QIYLSEVLD +A  SAQ KWDEK +   RKKLLSAL+ ISGY
Sbjct: 661 MMAMNDTAVSGNLQNEMVQIYLSEVLDLYAAKSAQQKWDEKDHPPERKKLLSALESISGY 720

Query: 721 HPEVLLKRLPSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQ 780
            P+ LLKRLP DALYEERA++LGKMNQHELALS+YVHK+H P+ AL+YCDR+YESV Y  
Sbjct: 721 SPQPLLKRLPRDALYEERAVILGKMNQHELALSIYVHKLHAPDLALAYCDRIYESVTYLP 780

Query: 781 STKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPK-LGSGPSFKVKGGRAAK 840
           S K S NIYLT+LQIYLNP+++ K+F KRI  L S ++  T K + S  S K KGGR +K
Sbjct: 781 SGKPSSNIYLTVLQIYLNPKKSAKDFAKRIVALGSFESSDTTKMMDSVLSSKAKGGR-SK 840

Query: 841 KIAAIEGAEDMRISL-SNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQA 900
           KI AIEGAEDMR+ L S+TDSGRSD DT+E  EEG S++M+ E LDLLSQRW+RINGAQA
Sbjct: 841 KIVAIEGAEDMRVGLSSSTDSGRSDVDTEEPLEEGDSTVMISEVLDLLSQRWERINGAQA 900

Query: 901 LKLLPKETKLQNLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSYTY 942
           LKLLP+ETKL NLL FL PLLR SSEA+RN SVIKSLRQSENLQ KE+ Y +
Sbjct: 901 LKLLPRETKLHNLLPFLAPLLRNSSEAHRNFSVIKSLRQSENLQVKEELYKH 942

BLAST of Sgr020593 vs. ExPASy Swiss-Prot
Match: Q8R5L3 (Vam6/Vps39-like protein OS=Mus musculus OX=10090 GN=Vps39 PE=1 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 6.4e-74
Identity = 248/975 (25.44%), Postives = 450/975 (46.15%), Query Frame = 0

Query: 2   VHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKSM 61
           +H A++   +L+  P +I+ + ++   L +G   G L +Y       D  P      +S 
Sbjct: 1   MHDAFEPVPILEKLPLQIDCLAAWEEWLLVGTKQGHLLLYRIRK---DVVPADVASPESG 60

Query: 62  ELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSE-SIAFHKLPNLETLAVITKAKG 121
              +    LE++   FS++ +  + V+   ++L++L E +I  H L   + +  ++KAKG
Sbjct: 61  SCNRFEVTLEKSNKNFSKK-IQQIHVVSQFKILVSLLENNIYVHDLLTFQQITTVSKAKG 120

Query: 122 ANVYSWD-------DRRGFLCFARQKRVCIFRHDGGRGFVEVK-EFGVPDTVKSMSWCGE 181
           A++++ D       +    +C A +K++ ++     R F E++ +F VPD  KSM+WC  
Sbjct: 121 ASLFTCDLQHTETGEEVLRMCVAVRKKLQLYFWK-DREFHELQGDFSVPDVPKSMAWCEN 180

Query: 182 NICLGIRREYVILNAT-SGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKL 241
           +IC+G +R+Y ++     G++ ++FP+G+   PLV  L  G++ +G+D++ V +++ G  
Sbjct: 181 SICVGFKRDYYLIRVDGKGSIKELFPTGKQLEPLVAPLADGKVAVGQDDLTVVLNEEGIC 240

Query: 242 LQEGRICWSEAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIH-SKHA 301
            Q+  + W++ P  +  Q PY +A+LPRY+EIR+L  P  L+Q+I L+  R +     + 
Sbjct: 241 TQKCALNWTDIPVAMEHQPPYIVAVLPRYVEIRTL-EPRLLVQSIELQRPRFITSGGSNI 300

Query: 302 LVVGLDNSAYGLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIH---I 361
           + V  ++  + L PVP+  QI QL     FE AL L ++    DS     K+  IH    
Sbjct: 301 IYVASNHFVWRLIPVPMATQIQQLLQDKQFELALQLAEMKDDSDSE----KQQQIHHIKN 360

Query: 362 RYAHYLFDNGSYEEAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHL 421
            YA  LF    ++E+M+ F+    D T+V+  YP +      L T+  K           
Sbjct: 361 LYAFNLFCQKRFDESMQVFAKLGTDPTHVMGLYPDL------LPTDYRK----------- 420

Query: 422 SRGSSGFSDDMESPPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGT 481
                           QL   +   TL   ++     +ALI +L +KR  +++K      
Sbjct: 421 ----------------QLQYPNPLPTLSGAEL-EKAHLALIDYLTQKRSQLVKKLND--- 480

Query: 482 EEVVLDAVGDRYKKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPN 541
                    D    +       P     +++  I+DT LL+  L T  +  A  L    N
Sbjct: 481 --------SDHQSSTSPLMEGTPTIKSKKKLLQIIDTTLLKCYLHTNVALVAPLLRLENN 540

Query: 542 YCDIKICEEILQKSNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPE 601
           +C I+  E +L+K++ YS L+ LY+   +H +AL++   LV++SK   S  +       E
Sbjct: 541 HCHIEESEHVLKKAHKYSELIILYEKKGLHEKALQV---LVDQSKKANSPLK-----GHE 600

Query: 602 MIIDYLKPLCGTDPILVLEFSMTVLESCPTQTIELFLSG-----NIPADLVNSYLKQHAP 661
             + YL+ L   +  L+  +S+ VL   P   +++F        ++P D V ++L ++  
Sbjct: 601 RTVQYLQHLGTENLHLIFSYSVWVLRDFPEDGLKIFTEDLPEVESLPRDRVLNFLIENFK 660

Query: 662 NLQATYLELMLAMNESSISGNLQNEMLQIYLSEVLDWHAD-----------LSAQHKWDE 721
            L   YLE ++ + E +      N ++Q+Y  +V     D           + A  +  E
Sbjct: 661 ALAIPYLEHIIHVWEET-GSQFHNCLIQLYCEKVQSLMKDYLLSLPTGKSPVPAGEEGGE 720

Query: 722 KTYSSTRKKLLSALDCISGYHPEVLLKRLPSDALYEERAILLGKMNQHELALSLYVHKIH 781
                 R+KLL  L+  S Y P  L+   P D L EERA+LLG+M +HE AL +YVH + 
Sbjct: 721 --LGEYRQKLLMFLEISSHYDPGRLICDFPFDGLLEERALLLGRMGKHEQALFIYVHVLK 780

Query: 782 VPEWALSYCDRVYESVAYQQSTKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMG 841
             + A  YC + Y+     Q+ + + ++YL+LL++YL+P                     
Sbjct: 781 DTKMAKEYCHKHYD-----QNKEGNKDVYLSLLRMYLSP--------------------- 840

Query: 842 TPKLGSGPSFKVKGGRAAKKIAAIEGAEDMRISLSNTDSGRSDGDTDETGEEGSSSIMLD 901
                  PS    G               +++ L    +                   L 
Sbjct: 841 -------PSIHCLG--------------PIKLELLEPQAN------------------LQ 844

Query: 902 EALDLLSQRWDRINGAQALKLLPKETKLQNLLQFLGPLLRKSSEAYRNSSVIKSLRQSEN 947
            AL +L   + +++  +A+ LLP  T++ ++  FL  +L ++++  R + V+K+L  +E 
Sbjct: 901 AALQVLELHYSKLDTTKAINLLPANTQINDIRIFLEKVLEENAQKKRFNQVLKNLLHAEF 844

BLAST of Sgr020593 vs. ExPASy Swiss-Prot
Match: Q96JC1 (Vam6/Vps39-like protein OS=Homo sapiens OX=9606 GN=VPS39 PE=1 SV=2)

HSP 1 Score: 277.3 bits (708), Expect = 9.3e-73
Identity = 249/973 (25.59%), Postives = 448/973 (46.04%), Query Frame = 0

Query: 2   VHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKSM 61
           +H A++   +L+  P +I+ + ++   L +G   G L +Y       D  P      +S 
Sbjct: 1   MHDAFEPVPILEKLPLQIDCLAAWEEWLLVGTKQGHLLLYRIRK---DVVPADVASPESG 60

Query: 62  ELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSE-SIAFHKLPNLETLAVITKAKG 121
              +    LE++   FS++ +  + V+   ++L++L E +I  H L   + +  ++KAKG
Sbjct: 61  SCNRFEVTLEKSNKNFSKK-IQQIHVVSQFKILVSLLENNIYVHDLLTFQQITTVSKAKG 120

Query: 122 ANVYSWD-------DRRGFLCFARQKRVCIFRHDGGRGFVEVK-EFGVPDTVKSMSWCGE 181
           A++++ D       +    +C A +K++ ++     R F E++ +F VPD  KSM+WC  
Sbjct: 121 ASLFTCDLQHTETGEEVLRMCVAVKKKLQLYFWK-DREFHELQGDFSVPDVPKSMAWCEN 180

Query: 182 NICLGIRREYVILNAT-SGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKL 241
           +IC+G +R+Y ++     G++ ++FP+G+   PLV  L  G++ +G+D++ V +++ G  
Sbjct: 181 SICVGFKRDYYLIRVDGKGSIKELFPTGKQLEPLVAPLADGKVAVGQDDLTVVLNEEGIC 240

Query: 242 LQEGRICWSEAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIH-SKHA 301
            Q+  + W++ P  +  Q PY IA+LPRY+EIR+   P  L+Q+I L+  R +     + 
Sbjct: 241 TQKCALNWTDIPVAMEHQPPYIIAVLPRYVEIRTF-EPRLLVQSIELQRPRFITSGGSNI 300

Query: 302 LVVGLDNSAYGLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIH---I 361
           + V  ++  + L PVP+  QI QL     FE AL L ++    DS     K+  IH    
Sbjct: 301 IYVASNHFVWRLIPVPMATQIQQLLQDKQFELALQLAEMKDDSDSE----KQQQIHHIKN 360

Query: 362 RYAHYLFDNGSYEEAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHL 421
            YA  LF    ++E+M+ F+    D T+V+  YP +      L T+  K +      P L
Sbjct: 361 LYAFNLFCQKRFDESMQVFAKLGTDPTHVMGLYPDL------LPTDYRKQLQYPNPLPVL 420

Query: 422 SRGSSGFSDDMESPPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGT 481
           S                          E +K +    +ALI +L +KR  +++K      
Sbjct: 421 SGA------------------------ELEKAH----LALIDYLTQKRSQLVKKLND--- 480

Query: 482 EEVVLDAVGDRYKKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPN 541
                    D    +       P     +++  I+DT LL+  L T  +  A  L    N
Sbjct: 481 --------SDHQSSTSPLMEGTPTIKSKKKLLQIIDTTLLKCYLHTNVALVAPLLRLENN 540

Query: 542 YCDIKICEEILQKSNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPE 601
           +C I+  E +L+K++ YS L+ LY+   +H +AL++   LV++SK   S  +       E
Sbjct: 541 HCHIEESEHVLKKAHKYSELIILYEKKGLHEKALQV---LVDQSKKANSPLK-----GHE 600

Query: 602 MIIDYLKPLCGTDPILVLEFSMTVLESCPTQTIELFLSG-----NIPADLVNSYLKQHAP 661
             + YL+ L   +  L+  +S+ VL   P   +++F        ++P D V  +L ++  
Sbjct: 601 RTVQYLQHLGTENLHLIFSYSVWVLRDFPEDGLKIFTEDLPEVESLPRDRVLGFLIENFK 660

Query: 662 NLQATYLELMLAMNESSISGNLQNEMLQIYLSEV--------LDWHADLSAQHKWDEK-T 721
            L   YLE ++ + E +      N ++Q+Y  +V        L + A  +     +E+  
Sbjct: 661 GLAIPYLEHIIHVWEET-GSRFHNCLIQLYCEKVQGLMKEYLLSFPAGKTPVPAGEEEGE 720

Query: 722 YSSTRKKLLSALDCISGYHPEVLLKRLPSDALYEERAILLGKMNQHELALSLYVHKIHVP 781
               R+KLL  L+  S Y P  L+   P D L EERA+LLG+M +HE AL +YVH +   
Sbjct: 721 LGEYRQKLLMFLEISSYYDPGRLICDFPFDGLLEERALLLGRMGKHEQALFIYVHILKDT 780

Query: 782 EWALSYCDRVYESVAYQQSTKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTP 841
             A  YC + Y+     ++   + ++YL+LL++YL+P                       
Sbjct: 781 RMAEEYCHKHYD-----RNKDGNKDVYLSLLRMYLSP----------------------- 840

Query: 842 KLGSGPSFKVKGGRAAKKIAAIEGAEDMRISLSNTDSGRSDGDTDETGEEGSSSIMLDEA 901
                PS    G               +++ L    +                   L  A
Sbjct: 841 -----PSIHCLG--------------PIKLELLEPKAN------------------LQAA 844

Query: 902 LDLLSQRWDRINGAQALKLLPKETKLQNLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQ 947
           L +L     +++  +AL LLP  T++ ++  FL  +L ++++  R + V+K+L  +E L+
Sbjct: 901 LQVLELHHSKLDTTKALNLLPANTQINDIRIFLEKVLEENAQKKRFNQVLKNLLHAEFLR 844

BLAST of Sgr020593 vs. ExPASy Swiss-Prot
Match: A4IG72 (Transforming growth factor-beta receptor-associated protein 1 homolog OS=Danio rerio OX=7955 GN=tgfbrap1 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 8.5e-34
Identity = 169/651 (25.96%), Postives = 276/651 (42.40%), Query Frame = 0

Query: 155 VKEFGVPDTVKSMSWCGENICLGIRREYVILNATSGALTDVFP-SGRLAPPLVVSLPSGE 214
           +KE   P+   ++S  G NICL +  +Y+ILN ++GA  D+FP       P+V  +   E
Sbjct: 161 LKEVTTPEQPCALSLDGYNICLALSTQYMILNYSTGASQDLFPYDCEERKPIVKRIGREE 220

Query: 215 LLL-GKDNIGVFVDQNGKLLQEGRICWSEAPSVIVIQKPYAIALLPRYIEIRSLRSPYAL 274
            LL     +G+F +  G + Q   + WSE      +  PY +AL   ++ + S+     L
Sbjct: 221 FLLAAPGGLGMFANAEG-ISQRAPVSWSENVIAAAVCFPYVVALDEGFVTVHSMLD-QQL 280

Query: 275 IQTIVLRNGRHLIHSKHALVVGLDNSAYGLFPVPLGAQIVQLTASGNFEEALALCKLLPP 334
            QT+  R+G+ L   +  +VV    + Y L P+PL  QI  L AS   EEAL L +    
Sbjct: 281 KQTLSFRDGQLLQDFEGKVVVASSKAVYMLVPLPLERQIQDLLASHRVEEALTLTE---A 340

Query: 335 EDSNLRSAKESSIH---IRYAHYL-FDNGSYEEAMEHFSASQVDITYVLPFYPSIVLPKT 394
              N+   K   +H   ++ A ++ F    + EA EHF   Q+D+  ++  YP ++LP +
Sbjct: 341 AQRNIPKEKYQILHRRILQQAGFIQFGQLQFLEAKEHFRKGQLDVRELISLYP-LLLPAS 400

Query: 395 TLVTETEKLMDMTLDGPHLSRGSSGFSDDMESPPHQLMESDENTTLESKKMNHNTLMALI 454
           +  T     +    D  HL++G                  D+      K+        LI
Sbjct: 401 SSFTRCHPPLHEFADLNHLTQG------------------DQEKVQRFKRF-------LI 460

Query: 455 KFLQKKRHIIIEKATAEGTEEVVLDAVGDRYKKSYKGRGNIPISSGAREMAAILDTALLQ 514
            +L    H +     A G  E V                               DTALL+
Sbjct: 461 SYL----HEVRSSDIANGFHEDV-------------------------------DTALLK 520

Query: 515 ALLFTGQSFAALELLKGPNYCDIKICEEILQKSNNYSALLELYKCNSMHREALKLLHQLV 574
               T    + L+LL   N C +      L+K + Y AL  LY  N     AL++  ++V
Sbjct: 521 LYAETSHE-SLLDLLASENACLLADSAPWLEKHHKYYALGLLYHYNGQDAAALQMWVKIV 580

Query: 575 EESKANESQAELIQKFKPEMIIDYLKPLCGTDPILVLEFSMTVLESCPTQTIELFLS--- 634
                + ++ +L      E ++D+L      D  LV   +   L+      +++F     
Sbjct: 581 NGDLQDSTRPDLF-----EYVVDFLSFCSNLD--LVWRHADWALQKDQKIGVQIFTKRPT 640

Query: 635 -----GNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISGNLQNEMLQIYLSEVLDWH 694
                G + AD V +YL++H+  L   YLE  L + +          +  +Y  +VL   
Sbjct: 641 SEERRGQLNADDVITYLQKHSQAL-LLYLE-HLVLEKKLQKEKYHTHLAVLYAEKVL--- 700

Query: 695 ADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL-PSDALYEERAILLGKMNQH 754
             L ++    E+  S+ R+KL   L   + Y  ++LL ++  S+ L  ERA L GK+ +H
Sbjct: 701 -GLISRPSTSEEQLSAARQKLQRLLKESNLYRVQLLLGKIQDSELLLLERATLHGKLEEH 727

Query: 755 ELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIYLTLLQIYLNP 791
           + AL + VH++     A  YC     S    Q +    N++  LL +YL+P
Sbjct: 761 DKALHVLVHQLKDSSAAEEYCSWASAS----QDSSYRQNLFHQLLSVYLDP 727

BLAST of Sgr020593 vs. ExPASy Swiss-Prot
Match: Q8WUH2 (Transforming growth factor-beta receptor-associated protein 1 OS=Homo sapiens OX=9606 GN=TGFBRAP1 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 2.6e-30
Identity = 231/952 (24.26%), Postives = 362/952 (38.03%), Query Frame = 0

Query: 19  IESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKSMELQ---KEPYVLERNVT 78
           IE +E  G  L++G +D  +  +  E   V   P +   +K ++     K+P    R  +
Sbjct: 27  IECVECCGRDLYVGTNDCFVYHFLLEERPVPAGPATFTATKQLQRHLGFKKPVNELRAAS 86

Query: 79  GFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKGA-------NVYSWDD 138
             +R             LL+    SI+   + NLE +    + KGA       N  S D 
Sbjct: 87  ALNR-------------LLVLCDNSISLVNMLNLEPVPSGARIKGAATFALNENPVSGDP 146

Query: 139 RRGFLCFARQKRVCIFRHDGGRGFVE-VKEFGVPDTVKSMSWCGENICLGIRREYVILNA 198
               +C    KR  I         V+ VKE    +   +++  G  +CL +  +Y+I N 
Sbjct: 147 FCVEVCIISVKRRTIQMFLVYEDRVQIVKEVSTAEQPLAVAVDGHFLCLALTTQYIIHNY 206

Query: 199 TSGALTDVFP-SGRLAPPLVVSLPSGELLL-GKDNIGVFVDQNGKLLQEGRICWSEAPSV 258
           ++G   D+FP      PP+V  +   E LL G   +G+F    G + Q   + WSE    
Sbjct: 207 STGVSQDLFPYCSEERPPIVKRIGRQEFLLAGPGGLGMFATVAG-ISQRAPVHWSENVIG 266

Query: 259 IVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAYGLFPV 318
             +  PY IAL   +I + S+       QT+  + G  L   +  ++V      Y L P+
Sbjct: 267 AAVSFPYVIALDDEFITVHSMLDQQQK-QTLPFKEGHILQDFEGRVIVATSKGVYILVPL 326

Query: 319 PLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNG-------S 378
           PL  QI  L AS   EEAL L K         R+  +    + Y   L   G        
Sbjct: 327 PLEKQIQDLLASRRVEEALVLAK------GARRNIPKEKFQVMYRRILQQAGFIQFAQLQ 386

Query: 379 YEEAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDM 438
           + EA E F + Q+D+  ++  YP  +LP ++  T +                        
Sbjct: 387 FLEAKELFRSGQLDVRELISLYP-FLLPTSSSFTRS------------------------ 446

Query: 439 ESPPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDR 498
             P H+  + ++ T  + +KM            + KR ++        TE      V + 
Sbjct: 447 HPPLHEYADLNQLTQGDQEKM-----------AKCKRFLMSYLNEVRSTE------VANG 506

Query: 499 YKKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEIL 558
           YK+                    +DTALL+ L       + L+LL   N+C +      L
Sbjct: 507 YKED-------------------IDTALLK-LYAEADHDSLLDLLVTENFCLLTDSAAWL 566

Query: 559 QKSNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCG 618
           +K   Y AL  LY  N+    A++L   +V     + ++++L      E I+D+L   C 
Sbjct: 567 EKHKKYFALGLLYHYNNQDAAAVQLWVNIVNGDVQDSTRSDLY-----EYIVDFL-TYC- 626

Query: 619 TDPILVLEFSMTVLESCPTQTIELFLSGNI---------PADLVNSYLKQHAPNLQATYL 678
            D  LV  ++  VL+      +++F    +         P D++N  LK++ P     YL
Sbjct: 627 LDEELVWAYADWVLQKSEEVGVQVFTKRPLDEQQKNSFNPDDIINC-LKKY-PKALVKYL 686

Query: 679 ELMLAMNESSISGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCIS 738
           E  L +++          +  +YL EVL   A  SA  K  E T   T+ KL   L    
Sbjct: 687 E-HLVIDKRLQKEEYHTHLAVLYLEEVLLQRA--SASGKGAEAT--ETQAKLRRLLQKSD 746

Query: 739 GYHPEVLLKRLPSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAY 798
            Y    LL+RL    L  E AIL GK+ +HE AL + VH++     A  YC    E    
Sbjct: 747 LYRVHFLLERLQGAGLPMESAILHGKLGEHEKALHILVHELQDFAAAEDYCLWCSEG--- 800

Query: 799 QQSTKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAA 858
            +       ++ TLL IYL+                           +GP+         
Sbjct: 807 -RDPPHRQQLFHTLLAIYLH---------------------------AGPT--------- 800

Query: 859 KKIAAIEGAEDMRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQA 918
                   A ++ ++                            A+DLL++     + AQ 
Sbjct: 867 --------AHELAVA----------------------------AVDLLNRHATEFDAAQV 800

Query: 919 LKLLPKETKLQNLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSYTY 942
           L++LP    +Q L  FL   +R S  A R   V   L +SENL      YTY
Sbjct: 927 LQMLPDTWSVQLLCPFLMGAMRDSIHARRTMQVALGLARSENL-----IYTY 800

BLAST of Sgr020593 vs. ExPASy TrEMBL
Match: A0A6J1C5T4 (vam6/Vps39-like protein OS=Momordica charantia OX=3673 GN=LOC111008226 PE=4 SV=1)

HSP 1 Score: 1739.5 bits (4504), Expect = 0.0e+00
Identity = 890/939 (94.78%), Postives = 911/939 (97.02%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFELLKDNPSKIE+I SYGSKLFIGCSDGSLRIYSPE+SG DRSPPSEFHSKS
Sbjct: 1   MVHSAYDSFELLKDNPSKIEAIVSYGSKLFIGCSDGSLRIYSPESSGSDRSPPSEFHSKS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
           MELQKEPYVLE+NVTGFSRRSL+SMEV+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  MELQKEPYVLEKNVTGFSRRSLLSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPD VKSMSWCGENICLGI+R
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDIVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSGALTDVFP GRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGALTDVFPPGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPSV+VIQKPYA+ALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHS HALVVGL NSAY
Sbjct: 241 EAPSVVVIQKPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSNHALVVGLGNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQ TASGNFEEALALCKLLPPEDS+LRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQFTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPSIVLPKTTLVTETEKLMD+TLDGPHLSRGSSGFSDDMES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLVTETEKLMDLTLDGPHLSRGSSGFSDDMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           PPHQL+ESDEN TLESKKMNHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PPHQLVESDENATLESKKMNHNTLMALIKFLQKKRHSIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQS AALELLKGPNYCD+KICEEILQK
Sbjct: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSLAALELLKGPNYCDVKICEEILQK 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           +N+YSALLELYKCNSMHREALKLLHQLVEESKANES  ELIQKF PEMIIDYLKPLCGTD
Sbjct: 541 NNHYSALLELYKCNSMHREALKLLHQLVEESKANES--ELIQKFNPEMIIDYLKPLCGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSM VLESCPTQTIELFLSGNIPADLVN YLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMIVLESCPTQTIELFLSGNIPADLVNFYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEK YSSTRKKLLSALD ISGYHPEVLLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKIYSSTRKKLLSALDSISGYHPEVLLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           PSDALYEERAILLGKMNQHELALSLYVHKIHV E ALSYCDRVYESVAYQQSTKSSGNIY
Sbjct: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVSELALSYCDRVYESVAYQQSTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           LTLLQIYLNPRRTTKNFEKRITNLTSPQ MGTPKLGSGP+FKVKGGRAA+KIAAIEGAED
Sbjct: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQTMGTPKLGSGPTFKVKGGRAARKIAAIEGAED 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
           M+IS SNTDSGRSDGDTDE GEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 MKISHSNTDSGRSDGDTDEMGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSY 940
           NL QFLGPLLRKSSEAYRNS VIKSLRQSENLQ +++ Y
Sbjct: 901 NLRQFLGPLLRKSSEAYRNSLVIKSLRQSENLQVRDELY 937

BLAST of Sgr020593 vs. ExPASy TrEMBL
Match: A0A6J1J1I5 (vam6/Vps39-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482579 PE=4 SV=1)

HSP 1 Score: 1704.1 bits (4412), Expect = 0.0e+00
Identity = 873/939 (92.97%), Postives = 904/939 (96.27%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP++SG DRSP S+FHS S
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPKSSGSDRSPTSDFHSMS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
           MELQKEPYVLE+NVTGFSRRSLVSM+V+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  MELQKEPYVLEKNVTGFSRRSLVSMKVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR
Sbjct: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSGALT+VFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGALTNVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPSV+VIQKPYAIALLPRYIEIRSL SPYALIQTIVLRNGRHLI S HALVVGLDNSAY
Sbjct: 241 EAPSVVVIQKPYAIALLPRYIEIRSLWSPYALIQTIVLRNGRHLIDSNHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDS+LRSAKESSIHIRYAH LFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHNLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPSI LPKTTL+TETEKLMDMTLD PHLS GSSGFSD+MES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIALPKTTLITETEKLMDMTLDDPHLSGGSSGFSDEMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           PPHQL+ESD NT+LESKK+NHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PPHQLLESDGNTSLESKKVNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKG NYCD+KICEEILQK
Sbjct: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           + +YSALLELY+CNSMHREALKLLHQLVEESK +ESQ EL QKFKPEMIIDYLK +CGTD
Sbjct: 541 NKHYSALLELYRCNSMHREALKLLHQLVEESKTSESQTELTQKFKPEMIIDYLKTICGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVLDW+ADLSAQHKWDEKTYS TRKKLLSAL+ ISGYHPE+LLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSPTRKKLLSALETISGYHPEILLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           P DAL EERAILLGKMNQHELALSLYVHKIH PE ALSYCDRVYESVA QQSTKSSGNIY
Sbjct: 721 PPDALSEERAILLGKMNQHELALSLYVHKIHAPELALSYCDRVYESVANQQSTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           LTLLQIYLNPRRTTK FEKRITNLTSPQN GTPKLGSGPS KVKG RA+KKIAAIEGAED
Sbjct: 781 LTLLQIYLNPRRTTKYFEKRITNLTSPQNTGTPKLGSGPSSKVKGSRASKKIAAIEGAED 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            +ISLSNT+S RSDGDTDETGEEGSSSIMLDEA+DLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 TKISLSNTESSRSDGDTDETGEEGSSSIMLDEAMDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSY 940
           NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQ +++ Y
Sbjct: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELY 939

BLAST of Sgr020593 vs. ExPASy TrEMBL
Match: A0A5A7TMM2 (Vam6/Vps39-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00280 PE=4 SV=1)

HSP 1 Score: 1701.4 bits (4405), Expect = 0.0e+00
Identity = 875/939 (93.18%), Postives = 906/939 (96.49%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP +S  DRS  S+FH +S
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPNSSASDRSSSSDFHPRS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            ELQKEPYVLE+NV+GFSRRSLVSMEV+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  TELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPSV+VIQ PYA+ALLPRYIEIRSLRSPYALIQTIVLRNGRHLI SKHALVVGLDNSAY
Sbjct: 241 EAPSVVVIQNPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDS+LRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPSIVLPKTTLVTETEKL+D  LD PHLSRGSSGFSDDMES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLVTETEKLVD--LDDPHLSRGSSGFSDDMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           P HQL+ESDENT+LESKK+NHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PVHQLLESDENTSLESKKVNHNTLMALIKFLQKKRHSIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKG NYCD+KICEEILQK
Sbjct: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           + +YSALLELY+CNSMHREALKLLHQLVEESKANESQ EL QKFKPEMIIDYLKPLCGTD
Sbjct: 541 NKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTEL-QKFKPEMIIDYLKPLCGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVL+W+ADLSAQ+KWDEK YSSTRKKLLSAL+ ISGY PEVLLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLEWYADLSAQNKWDEKIYSSTRKKLLSALESISGYQPEVLLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           PSDAL EERAILLGKMNQHELALSLYVHKIHVPE ALSYCDRVYESVA QQ TKSSGNIY
Sbjct: 721 PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGR+AKKIAAIEGAED
Sbjct: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRSAKKIAAIEGAED 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            ++SLSNTDS RSDGDTDE GEEGSSSIMLDEAL+LLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 TKVSLSNTDSSRSDGDTDEPGEEGSSSIMLDEALNLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSY 940
           NLLQF+GPLLRKSSEAYRNSSVIKSLRQSENLQ +++ Y
Sbjct: 901 NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELY 936

BLAST of Sgr020593 vs. ExPASy TrEMBL
Match: A0A1S3BX35 (vam6/Vps39-like protein OS=Cucumis melo OX=3656 GN=LOC103494389 PE=4 SV=1)

HSP 1 Score: 1701.4 bits (4405), Expect = 0.0e+00
Identity = 875/939 (93.18%), Postives = 906/939 (96.49%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP +S  DRS  S+FH +S
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPNSSASDRSSSSDFHPRS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            ELQKEPYVLE+NV+GFSRRSLVSMEV+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  TELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPSV+VIQ PYA+ALLPRYIEIRSLRSPYALIQTIVLRNGRHLI SKHALVVGLDNSAY
Sbjct: 241 EAPSVVVIQNPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDS+LRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPSIVLPKTTLVTETEKL+D  LD PHLSRGSSGFSDDMES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLVTETEKLVD--LDDPHLSRGSSGFSDDMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           P HQL+ESDENT+LESKK+NHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PVHQLLESDENTSLESKKVNHNTLMALIKFLQKKRHSIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKG NYCD+KICEEILQK
Sbjct: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           + +YSALLELY+CNSMHREALKLLHQLVEESKANESQ EL QKFKPEMIIDYLKPLCGTD
Sbjct: 541 NKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTEL-QKFKPEMIIDYLKPLCGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVL+W+ADLSAQ+KWDEK YSSTRKKLLSAL+ ISGY PEVLLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLEWYADLSAQNKWDEKIYSSTRKKLLSALESISGYQPEVLLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           PSDAL EERAILLGKMNQHELALSLYVHKIHVPE ALSYCDRVYESVA QQ TKSSGNIY
Sbjct: 721 PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGR+AKKIAAIEGAED
Sbjct: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRSAKKIAAIEGAED 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            ++SLSNTDS RSDGDTDE GEEGSSSIMLDEAL+LLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 TKVSLSNTDSSRSDGDTDEPGEEGSSSIMLDEALNLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSY 940
           NLLQF+GPLLRKSSEAYRNSSVIKSLRQSENLQ +++ Y
Sbjct: 901 NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELY 936

BLAST of Sgr020593 vs. ExPASy TrEMBL
Match: A0A6J1FTD4 (vam6/Vps39-like protein OS=Cucurbita moschata OX=3662 GN=LOC111447123 PE=4 SV=1)

HSP 1 Score: 1699.5 bits (4400), Expect = 0.0e+00
Identity = 874/996 (87.75%), Postives = 920/996 (92.37%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVHSAYDSFEL KDNPSKIESIESYGSKLFIGCSDGSLRIYSP +SG DRS  S+FHS S
Sbjct: 1   MVHSAYDSFELFKDNPSKIESIESYGSKLFIGCSDGSLRIYSPVSSGPDRSLSSDFHSMS 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
           MELQKEPY+LE+NV GFSRRSLVSM+V+DSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  MELQKEPYILEKNVKGFSRRSLVSMKVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121 ANTYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILNATSG LTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS
Sbjct: 181 EYVILNATSGTLTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAPSV+VIQ PYA+ALLPRYIE+RSLRSPY+LIQTIVLRNGRHLI SKHA VVGLDNSAY
Sbjct: 241 EAPSVVVIQNPYAVALLPRYIEVRSLRSPYSLIQTIVLRNGRHLIDSKHASVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDS LRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSILRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDMES 420
           EAMEHF ASQVDITYVLPFYPS+VLPKTTL+TETEKLMDMTLD PHLSR SS FSDDMES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSLVLPKTTLITETEKLMDMTLDDPHLSRVSSDFSDDMES 420

Query: 421 PPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRYK 480
           PPHQL+ESDE++TLESKKMNHNTLMALIKFLQKKRH IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PPHQLLESDEHSTLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQK 540
           KSYK R N+PISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQ+
Sbjct: 481 KSYKARANVPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDIKICEEILQE 540

Query: 541 SNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIIDYLKPLCGTD 600
           +N+YSALLELYKCNSMHREALK+LHQLVEESKAN+SQ E IQ FKPEMIIDYLK LCGTD
Sbjct: 541 NNHYSALLELYKCNSMHREALKILHQLVEESKANKSQPEPIQMFKPEMIIDYLKTLCGTD 600

Query: 601 PILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           P+LVLEFSM +LESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQ TYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMPILESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQGTYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGYHPEVLLKRL 720
           SGNLQNEMLQIYLSEVL+W+ADL+AQHKWDEKTYSSTRKKLLSAL+ ISGYHPEVLLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLEWYADLTAQHKWDEKTYSSTRKKLLSALETISGYHPEVLLKRL 720

Query: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQSTKSSGNIY 780
           PSDALYEERAILLGK+NQHELALSLYVHKIHVPE ALSYCDRVYESVAYQQSTKSSGNIY
Sbjct: 721 PSDALYEERAILLGKLNQHELALSLYVHKIHVPELALSYCDRVYESVAYQQSTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           L LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPK+GSGPSFKVKGGRAAKKI AIEGAE+
Sbjct: 781 LILLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKIGSGPSFKVKGGRAAKKIVAIEGAEN 840

Query: 841 MRISLSNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            +ISLS TDSGR+DGDTDET EEGSS IMLDEALDLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 TKISLSKTDSGRTDGDTDETCEEGSSPIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSYTYTAIGCR---NCCCTV---- 960
           NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQ ++  Y       +   N  C++    
Sbjct: 901 NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQVRDDLYKQRKPEIKVTSNSVCSLCNKK 960

Query: 961 ---TAYRSFPTAVPLPELLLKKKSYLLLPTEASQPL 987
              + +  +PT + L   +  + S  +       P+
Sbjct: 961 IGTSVFAVYPTGITLVHFVCFRDSQSMKAVSKGSPI 996

BLAST of Sgr020593 vs. TAIR 10
Match: AT4G36630.1 (Vacuolar sorting protein 39 )

HSP 1 Score: 1321.2 bits (3418), Expect = 0.0e+00
Identity = 684/952 (71.85%), Postives = 803/952 (84.35%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPEASGVDRSPPSEFHSKS 60
           MVH+AYDSF+LLKD P++I+++ESYGSKLF GC DGSLRIYSP  S    S PSE H   
Sbjct: 1   MVHNAYDSFQLLKDCPARIDAVESYGSKLFAGCYDGSLRIYSPPESSA--SDPSELH--- 60

Query: 61  MELQKEPYVLERNVTGFSRRSLVSMEVLDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
               +E YVLE+ V GFS++ +V+MEVL SRELLL+LSESIAFH LPNLET+AVITKAKG
Sbjct: 61  ----QETYVLEKTVAGFSKKPIVAMEVLASRELLLSLSESIAFHGLPNLETVAVITKAKG 120

Query: 121 ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180
           AN YSWDDRRGFLCF+RQKRVC+F+HDGG GFVEV+++GVPDTVKS+SWCGENICLGI++
Sbjct: 121 ANAYSWDDRRGFLCFSRQKRVCVFKHDGGGGFVEVRDYGVPDTVKSISWCGENICLGIKK 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240
           EYVILN  +G L++VFPSGR+APPLV+SLPSGEL+LGK+NIGVFVDQNGKLLQ  RICWS
Sbjct: 181 EYVILNTANGTLSEVFPSGRVAPPLVISLPSGELILGKENIGVFVDQNGKLLQTERICWS 240

Query: 241 EAPSVIVIQKPYAIALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSKHALVVGLDNSAY 300
           EAP+ IVIQ PYAIALLPR +E+R LRSPY LIQTIVL+N R L+ S +A++VGLDNS Y
Sbjct: 241 EAPTSIVIQNPYAIALLPRRVEVRLLRSPYPLIQTIVLQNIRRLVKSNNAVIVGLDNSVY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSNLRSAKESSIHIRYAHYLFDNGSYE 360
            LFPV +GAQIVQLTASGNFEEALALCK+LPP++S+LR+AKESSIH R+AHYLF+NGSYE
Sbjct: 301 VLFPVSIGAQIVQLTASGNFEEALALCKVLPPDESSLRAAKESSIHTRFAHYLFENGSYE 360

Query: 361 EAMEHFSASQVDITYVLPFYPSIVLPKTTLVTETEKLMDMTLDGPHLSRGSSGFSDDME- 420
           EAMEHF ASQVDIT+VL  YPSI+LPKTT++ + +K++D++ D   LSRGSSG SDDME 
Sbjct: 361 EAMEHFLASQVDITHVLSMYPSIILPKTTIIPQPDKMVDISGDEASLSRGSSGISDDMES 420

Query: 421 SPPHQLMESDENTTLESKKMNHNTLMALIKFLQKKRHIIIEKATAEGTEEVVLDAVGDRY 480
           S P   +ES++N  LESKKM+HNTLMALIK+L K+R  +IEKAT+EGTEEV+ DAVG  Y
Sbjct: 421 SSPRYFLESEDNADLESKKMSHNTLMALIKYLLKRRPAVIEKATSEGTEEVISDAVGKTY 480

Query: 481 --------KKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGPNYCDI 540
                   KKS KGRG IP++SGAREMAAILDTALLQALL TGQS AA+ELLKG NY D+
Sbjct: 481 GANDSSKSKKSSKGRGMIPLNSGAREMAAILDTALLQALLHTGQSGAAIELLKGVNYSDV 540

Query: 541 KICEEILQKSNNYSALLELYKCNSMHREALKLLHQLVEESKANESQAELIQKFKPEMIID 600
           KICEEIL KS NYSALLEL+K NSMH EALKLL+QL +ESK N+SQ ++ Q F PE+II+
Sbjct: 541 KICEEILMKSKNYSALLELFKSNSMHHEALKLLNQLADESKTNQSQTDVTQIFSPELIIE 600

Query: 601 YLKPLCGTDPILVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLEL 660
           YLKPLC TDP+LVLE+SM VLESCPTQTI+LFLSGNI ADLVNSYLKQHAPN+Q  YLEL
Sbjct: 601 YLKPLCRTDPMLVLEYSMLVLESCPTQTIDLFLSGNISADLVNSYLKQHAPNMQGRYLEL 660

Query: 661 MLAMNESSISGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKTYSSTRKKLLSALDCISGY 720
           M+AMN++++SGNLQNEM+QIYLSEVLD +A  SAQ KWDEK +   RKKLLSAL+ ISGY
Sbjct: 661 MMAMNDTAVSGNLQNEMVQIYLSEVLDLYAAKSAQQKWDEKDHPPERKKLLSALESISGY 720

Query: 721 HPEVLLKRLPSDALYEERAILLGKMNQHELALSLYVHKIHVPEWALSYCDRVYESVAYQQ 780
            P+ LLKRLP DALYEERA++LGKMNQHELALS+YVHK+H P+ AL+YCDR+YESV Y  
Sbjct: 721 SPQPLLKRLPRDALYEERAVILGKMNQHELALSIYVHKLHAPDLALAYCDRIYESVTYLP 780

Query: 781 STKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPK-LGSGPSFKVKGGRAAK 840
           S K S NIYLT+LQIYLNP+++ K+F KRI  L S ++  T K + S  S K KGGR +K
Sbjct: 781 SGKPSSNIYLTVLQIYLNPKKSAKDFAKRIVALGSFESSDTTKMMDSVLSSKAKGGR-SK 840

Query: 841 KIAAIEGAEDMRISL-SNTDSGRSDGDTDETGEEGSSSIMLDEALDLLSQRWDRINGAQA 900
           KI AIEGAEDMR+ L S+TDSGRSD DT+E  EEG S++M+ E LDLLSQRW+RINGAQA
Sbjct: 841 KIVAIEGAEDMRVGLSSSTDSGRSDVDTEEPLEEGDSTVMISEVLDLLSQRWERINGAQA 900

Query: 901 LKLLPKETKLQNLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQEKEKSYTY 942
           LKLLP+ETKL NLL FL PLLR SSEA+RN SVIKSLRQSENLQ KE+ Y +
Sbjct: 901 LKLLPRETKLHNLLPFLAPLLRNSSEAHRNFSVIKSLRQSENLQVKEELYKH 942