Sgr020483 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr020483 vs. NCBI nr
Match: XP_022158146.1 (ubiquitin carboxyl-terminal hydrolase 23 isoform X1 [Momordica charantia])

HSP 1 Score: 1520.8 bits (3936), Expect = 0.0e+00
Identity = 796/964 (82.57%), Postives = 838/964 (86.93%), Query Frame = 0

Query: 1   MADGVVFKPKHDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLNP 60
           MADG VFKP+HDNPSDCPGTSAD LLSSLFQR ++FHPAR+SF GFDNGGGDFR ETLNP
Sbjct: 1   MADGFVFKPEHDNPSDCPGTSAD-LLSSLFQRRMDFHPARKSFNGFDNGGGDFRFETLNP 60

Query: 61  SSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGLQ 120
           SSS A KSGSNVDHSASKGKKMDGSELLENGLDPELSFEI+FRRI          GAGLQ
Sbjct: 61  SSSLAHKSGSNVDHSASKGKKMDGSELLENGLDPELSFEITFRRI----------GAGLQ 120

Query: 121 NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL 180
           NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL
Sbjct: 121 NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL 180

Query: 181 APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHKI 240
           APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSES SAYEKSLVHKI
Sbjct: 181 APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESPSAYEKSLVHKI 240

Query: 241 FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ 300
           FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ
Sbjct: 241 FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ 300

Query: 301 RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG 360
           RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG
Sbjct: 301 RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG 360

Query: 361 DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFYV 420
           DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDR VFEQQAYMLFYV
Sbjct: 361 DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRTVFEQQAYMLFYV 420

Query: 421 RDRRKVVLKKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKKLNGLSSDELR 480
           RDRRKVV KKS+D ALKENMK ST  NR  S+VNQGLKDNC QN TFEKKLNG +SDELR
Sbjct: 421 RDRRKVVQKKSIDIALKENMKMSTSSNRSDSVVNQGLKDNCAQNCTFEKKLNGPTSDELR 480

Query: 481 KESKDSSNIDPSKTTVLNGPSVRIETEQASKECSVPATISMPISSLKRVLPLKTFGKDII 540
           KESKDS N+ PSKT + NGPSV+IE EQAS ECSVP T+SMP  S + VLPLKTF +DI+
Sbjct: 481 KESKDSPNMGPSKTKISNGPSVQIEIEQASTECSVPVTVSMPTYSSRGVLPLKTFSEDIM 540

Query: 541 PNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNLIDANTS 600
           PNSSPAEDL SLP  MN+ F+ATNS  SLA+AA+IEI HVNRGLGV VAT LNLID NTS
Sbjct: 541 PNSSPAEDLTSLPVCMNNNFVATNSVSSLARAADIEIGHVNRGLGVLVATSLNLIDPNTS 600

Query: 601 AIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNSVGNLSS 660
           AIPQAELLVSHVQN  AA T+ESGCKT EKS PEILP+QP SE+SKVD  NQ  VGNLSS
Sbjct: 601 AIPQAELLVSHVQNGNAALTEESGCKTLEKSYPEILPDQPKSESSKVDIPNQKFVGNLSS 660

Query: 661 EDASNCKKLVPDESNEISSSSTMTDGSLLTKTLDFKNGRRLKRKALKYHVGSLHLSSNIL 720
           EDAS+CKKL+PDESN++S+SS MT   L+TKT D K+GRRLKRK LKYH+GSLHLSSNIL
Sbjct: 661 EDASDCKKLMPDESNKMSTSSIMTSELLVTKTPDSKHGRRLKRKNLKYHLGSLHLSSNIL 720

Query: 721 FKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEKAKSIYLVSTCKS 780
           FKASLSLCKK+KHKRKKRQSAGSKNLT+ESL   DGISSD GPSTSEKAKSIYLVSTCKS
Sbjct: 721 FKASLSLCKKRKHKRKKRQSAGSKNLTEESLSSTDGISSDLGPSTSEKAKSIYLVSTCKS 780

Query: 781 RKKAKHGSKE--------------------------EKQKEISVLATKNELNCSTDFLKR 840
           RKKAKHGS+E                            ++E S LATK +LNCS+D    
Sbjct: 781 RKKAKHGSREGNGISTKNEAHNVEELTDIVDKGSGKRSKEESSELATKKKLNCSSD---- 840

Query: 841 SEKEANHNKSIEANFSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGME 900
           S   ANH +SIE NF KDRK +AN+DG  CVLT GFQN+VVEQWDG KMPS EN F GME
Sbjct: 841 SIIVANHKESIEVNFPKDRKINANQDGFRCVLTNGFQNTVVEQWDGTKMPSFENGFSGME 900

Query: 901 NI-SIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRSGSANE 938
            I SIGYVADEWDEEYDQGKRKKIR+FKHSFGGPNPFQEIA KKSQ KKLKLDRSGSA E
Sbjct: 901 TIRSIGYVADEWDEEYDQGKRKKIREFKHSFGGPNPFQEIATKKSQFKKLKLDRSGSAKE 949

BLAST of Sgr020483 vs. NCBI nr
Match: XP_022158147.1 (ubiquitin carboxyl-terminal hydrolase 23 isoform X2 [Momordica charantia])

HSP 1 Score: 1498.8 bits (3879), Expect = 0.0e+00
Identity = 788/964 (81.74%), Postives = 830/964 (86.10%), Query Frame = 0

Query: 1   MADGVVFKPKHDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLNP 60
           MADG VFKP+HDNPSDCPGTSAD LLSSLFQR ++FHPAR+SF GFDNGGGDFR ETLNP
Sbjct: 1   MADGFVFKPEHDNPSDCPGTSAD-LLSSLFQRRMDFHPARKSFNGFDNGGGDFRFETLNP 60

Query: 61  SSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGLQ 120
           SSS A KSGSNVDHSASKGKKMDGSELLENGLDPELSFEI+FRRI          GAGLQ
Sbjct: 61  SSSLAHKSGSNVDHSASKGKKMDGSELLENGLDPELSFEITFRRI----------GAGLQ 120

Query: 121 NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL 180
           NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL
Sbjct: 121 NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL 180

Query: 181 APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHKI 240
           APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSES SAYEKSLVHKI
Sbjct: 181 APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESPSAYEKSLVHKI 240

Query: 241 FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ 300
           FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ
Sbjct: 241 FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ 300

Query: 301 RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG 360
           RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG
Sbjct: 301 RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG 360

Query: 361 DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFYV 420
           DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDR VFEQQAYMLFYV
Sbjct: 361 DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRTVFEQQAYMLFYV 420

Query: 421 RDRRKVVLKKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKKLNGLSSDELR 480
           RDRRKVV KKS+D ALKENMK ST  NR  S+VNQGLKDNC QN TFEKKLNG +SDELR
Sbjct: 421 RDRRKVVQKKSIDIALKENMKMSTSSNRSDSVVNQGLKDNCAQNCTFEKKLNGPTSDELR 480

Query: 481 KESKDSSNIDPSKTTVLNGPSVRIETEQASKECSVPATISMPISSLKRVLPLKTFGKDII 540
           KESKDS N+ PSKT + NGPSV+IE EQAS ECSVP T+SMP  S + VLPLKTF +DI+
Sbjct: 481 KESKDSPNMGPSKTKISNGPSVQIEIEQASTECSVPVTVSMPTYSSRGVLPLKTFSEDIM 540

Query: 541 PNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNLIDANTS 600
           PNSSPAEDL SLP  MN+ F+ATNS  SLA+AA+IEI HVNRGLGV VAT LNLID NTS
Sbjct: 541 PNSSPAEDLTSLPVCMNNNFVATNSVSSLARAADIEIGHVNRGLGVLVATSLNLIDPNTS 600

Query: 601 AIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNSVGNLSS 660
           AIPQA        N  AA T+ESGCKT EKS PEILP+QP SE+SKVD  NQ  VGNLSS
Sbjct: 601 AIPQA--------NGNAALTEESGCKTLEKSYPEILPDQPKSESSKVDIPNQKFVGNLSS 660

Query: 661 EDASNCKKLVPDESNEISSSSTMTDGSLLTKTLDFKNGRRLKRKALKYHVGSLHLSSNIL 720
           EDAS+CKKL+PDESN++S+SS MT   L+TKT D K+GRRLKRK LKYH+GSLHLSSNIL
Sbjct: 661 EDASDCKKLMPDESNKMSTSSIMTSELLVTKTPDSKHGRRLKRKNLKYHLGSLHLSSNIL 720

Query: 721 FKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEKAKSIYLVSTCKS 780
           FKASLSLCKK+KHKRKKRQSAGSKNLT+ESL   DGISSD GPSTSEKAKSIYLVSTCKS
Sbjct: 721 FKASLSLCKKRKHKRKKRQSAGSKNLTEESLSSTDGISSDLGPSTSEKAKSIYLVSTCKS 780

Query: 781 RKKAKHGSKE--------------------------EKQKEISVLATKNELNCSTDFLKR 840
           RKKAKHGS+E                            ++E S LATK +LNCS+D    
Sbjct: 781 RKKAKHGSREGNGISTKNEAHNVEELTDIVDKGSGKRSKEESSELATKKKLNCSSD---- 840

Query: 841 SEKEANHNKSIEANFSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGME 900
           S   ANH +SIE NF KDRK +AN+DG  CVLT GFQN+VVEQWDG KMPS EN F GME
Sbjct: 841 SIIVANHKESIEVNFPKDRKINANQDGFRCVLTNGFQNTVVEQWDGTKMPSFENGFSGME 900

Query: 901 NI-SIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRSGSANE 938
            I SIGYVADEWDEEYDQGKRKKIR+FKHSFGGPNPFQEIA KKSQ KKLKLDRSGSA E
Sbjct: 901 TIRSIGYVADEWDEEYDQGKRKKIREFKHSFGGPNPFQEIATKKSQFKKLKLDRSGSAKE 941

BLAST of Sgr020483 vs. NCBI nr
Match: XP_022158148.1 (ubiquitin carboxyl-terminal hydrolase 23 isoform X3 [Momordica charantia])

HSP 1 Score: 1464.1 bits (3789), Expect = 0.0e+00
Identity = 767/931 (82.38%), Postives = 808/931 (86.79%), Query Frame = 0

Query: 34  IEFHPARRSFKGFDNGGGDFRLETLNPSSSFAQKSGSNVDHSASKGKKMDGSELLENGLD 93
           ++FHPAR+SF GFDNGGGDFR ETLNPSSS A KSGSNVDHSASKGKKMDGSELLENGLD
Sbjct: 1   MDFHPARKSFNGFDNGGGDFRFETLNPSSSLAHKSGSNVDHSASKGKKMDGSELLENGLD 60

Query: 94  PELSFEISFRRIVSVSSMTSVKGAGLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNS 153
           PELSFEI+FRRI          GAGLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNS
Sbjct: 61  PELSFEITFRRI----------GAGLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNS 120

Query: 154 CHVAGFCALCAIQKHVSRALQSSGRILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLE 213
           CHVAGFCALCAIQKHVSRALQSSGRILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLE
Sbjct: 121 CHVAGFCALCAIQKHVSRALQSSGRILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLE 180

Query: 214 SMHRCCLPLGLPSESASAYEKSLVHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVK 273
           SMHRCCLPLGLPSES SAYEKSLVHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVK
Sbjct: 181 SMHRCCLPLGLPSESPSAYEKSLVHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVK 240

Query: 274 ADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNL 333
           ADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNL
Sbjct: 241 ADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNL 300

Query: 334 EEKIHKKIQFGPTLDLKPFVSGSYADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMW 393
           EEKIHKKIQFGPTLDLKPFVSGSYADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMW
Sbjct: 301 EEKIHKKIQFGPTLDLKPFVSGSYADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMW 360

Query: 394 YALDDNRVSHVGDRIVFEQQAYMLFYVRDRRKVVLKKSVDGALKENMKTSTGLNRMASIV 453
           YALDDNRVSHVGDR VFEQQAYMLFYVRDRRKVV KKS+D ALKENMK ST  NR  S+V
Sbjct: 361 YALDDNRVSHVGDRTVFEQQAYMLFYVRDRRKVVQKKSIDIALKENMKMSTSSNRSDSVV 420

Query: 454 NQGLKDNCVQNSTFEKKLNGLSSDELRKESKDSSNIDPSKTTVLNGPSVRIETEQASKEC 513
           NQGLKDNC QN TFEKKLNG +SDELRKESKDS N+ PSKT + NGPSV+IE EQAS EC
Sbjct: 421 NQGLKDNCAQNCTFEKKLNGPTSDELRKESKDSPNMGPSKTKISNGPSVQIEIEQASTEC 480

Query: 514 SVPATISMPISSLKRVLPLKTFGKDIIPNSSPAEDLPSLPRRMNSIFLATNSECSLAKAA 573
           SVP T+SMP  S + VLPLKTF +DI+PNSSPAEDL SLP  MN+ F+ATNS  SLA+AA
Sbjct: 481 SVPVTVSMPTYSSRGVLPLKTFSEDIMPNSSPAEDLTSLPVCMNNNFVATNSVSSLARAA 540

Query: 574 NIEISHVNRGLGVSVATPLNLIDANTSAIPQAELLVSHVQNDTAASTQESGCKTSEKSDP 633
           +IEI HVNRGLGV VAT LNLID NTSAIPQAELLVSHVQN  AA T+ESGCKT EKS P
Sbjct: 541 DIEIGHVNRGLGVLVATSLNLIDPNTSAIPQAELLVSHVQNGNAALTEESGCKTLEKSYP 600

Query: 634 EILPEQPISENSKVDTSNQNSVGNLSSEDASNCKKLVPDESNEISSSSTMTDGSLLTKTL 693
           EILP+QP SE+SKVD  NQ  VGNLSSEDAS+CKKL+PDESN++S+SS MT   L+TKT 
Sbjct: 601 EILPDQPKSESSKVDIPNQKFVGNLSSEDASDCKKLMPDESNKMSTSSIMTSELLVTKTP 660

Query: 694 DFKNGRRLKRKALKYHVGSLHLSSNILFKASLSLCKKKKHKRKKRQSAGSKNLTDESLFG 753
           D K+GRRLKRK LKYH+GSLHLSSNILFKASLSLCKK+KHKRKKRQSAGSKNLT+ESL  
Sbjct: 661 DSKHGRRLKRKNLKYHLGSLHLSSNILFKASLSLCKKRKHKRKKRQSAGSKNLTEESLSS 720

Query: 754 RDGISSDFGPSTSEKAKSIYLVSTCKSRKKAKHGSKE----------------------- 813
            DGISSD GPSTSEKAKSIYLVSTCKSRKKAKHGS+E                       
Sbjct: 721 TDGISSDLGPSTSEKAKSIYLVSTCKSRKKAKHGSREGNGISTKNEAHNVEELTDIVDKG 780

Query: 814 ---EKQKEISVLATKNELNCSTDFLKRSEKEANHNKSIEANFSKDRKQSANRDGLHCVLT 873
                ++E S LATK +LNCS+D    S   ANH +SIE NF KDRK +AN+DG  CVLT
Sbjct: 781 SGKRSKEESSELATKKKLNCSSD----SIIVANHKESIEVNFPKDRKINANQDGFRCVLT 840

Query: 874 QGFQNSVVEQWDGIKMPSSENEFIGMENI-SIGYVADEWDEEYDQGKRKKIRQFKHSFGG 933
            GFQN+VVEQWDG KMPS EN F GME I SIGYVADEWDEEYDQGKRKKIR+FKHSFGG
Sbjct: 841 NGFQNTVVEQWDGTKMPSFENGFSGMETIRSIGYVADEWDEEYDQGKRKKIREFKHSFGG 900

Query: 934 PNPFQEIAMKKSQLKKLKLDRSGSANEPFRI 938
           PNPFQEIA KKSQ KKLKLDRSGSA EPFRI
Sbjct: 901 PNPFQEIATKKSQFKKLKLDRSGSAKEPFRI 917

BLAST of Sgr020483 vs. NCBI nr
Match: XP_038889714.1 (ubiquitin carboxyl-terminal hydrolase 23 isoform X1 [Benincasa hispida])

HSP 1 Score: 1382.9 bits (3578), Expect = 0.0e+00
Identity = 747/964 (77.49%), Postives = 801/964 (83.09%), Query Frame = 0

Query: 1   MADGVVFKPK-HDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLN 60
           MADGVVFKP   DN SDCP TS D L+ SLFQ+ +E+ PARR+FKGFDNGGGDF L TLN
Sbjct: 1   MADGVVFKPGFDDNQSDCPETSTD-LVFSLFQKRVEYVPARRTFKGFDNGGGDFELTTLN 60

Query: 61  PSSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGL 120
           PSSSF QKSGSNVDH+A KGKK+DGSELLENGLDPELSFEI+FRRI          GAGL
Sbjct: 61  PSSSFGQKSGSNVDHTAPKGKKLDGSELLENGLDPELSFEITFRRI----------GAGL 120

Query: 121 QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI 180
           QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI
Sbjct: 121 QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI 180

Query: 181 LAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHK 240
           LAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMH+CCLPLGLPSES SAYEKSLVHK
Sbjct: 181 LAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHKCCLPLGLPSESPSAYEKSLVHK 240

Query: 241 IFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQC 300
           IFGGRLRSQVKCMQCS+CSN FDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQC
Sbjct: 241 IFGGRLRSQVKCMQCSFCSNKFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQC 300

Query: 301 QRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYAD 360
           QRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKI FGPTLDL PFVSGSYAD
Sbjct: 301 QRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIHFGPTLDLTPFVSGSYAD 360

Query: 361 GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFY 420
           G LKYTLYGVLVHHGGSTRSGHYYCYVRTSS MWYALDDNRVSHVGDR V+EQQAYMLFY
Sbjct: 361 GALKYTLYGVLVHHGGSTRSGHYYCYVRTSSAMWYALDDNRVSHVGDRTVYEQQAYMLFY 420

Query: 421 VRDRRKVVLKKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKKLNGLSSDEL 480
           VRDRRKVV KK VD ALKENM+TST LNR  SIVN+ LKDN VQN TFEKKLN   +D L
Sbjct: 421 VRDRRKVVPKKPVDVALKENMRTSTNLNRTDSIVNRDLKDNRVQNCTFEKKLNEPINDGL 480

Query: 481 RKESKDSSNIDPSKTTVLNGPSVRIETEQASKECSVPATISMPISSLKRVLPLKTFGKDI 540
            KESKDSSN  PSK T+ N PSV+IETE ASK C VP T+SM ISS K V P KT  KDI
Sbjct: 481 IKESKDSSNEGPSK-TISNKPSVQIETELASKGCLVPETVSMAISS-KEVSPQKTSNKDI 540

Query: 541 IPNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNLIDANT 600
           +PNSS A +LP LPR MNS    T+SE SLAK  +I+I+HV+RGLGV V+T LNLIDANT
Sbjct: 541 VPNSSSAVNLPMLPRCMNSNLHVTSSESSLAKVDHIDINHVDRGLGVQVSTSLNLIDANT 600

Query: 601 SAIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNSVGNLS 660
           SAI QA        ND AASTQESGCKT EKSDPEILP Q + E+SK   SN  SVGN +
Sbjct: 601 SAILQA--------NDNAASTQESGCKTLEKSDPEILPSQQMLESSKFGDSNHISVGNST 660

Query: 661 SEDASNCKKLVPDESNEISSSSTMTDGSLLTKTLDFKNGRRLKRKALKYHVGSLHLSSNI 720
           S D SNCKKL+PDESN+I SSSTM +G LL+KTLD K+GRRLKRK LKYH+GSLHLSSNI
Sbjct: 661 SGDVSNCKKLMPDESNKI-SSSTMVEGPLLSKTLDSKHGRRLKRKHLKYHLGSLHLSSNI 720

Query: 721 LFKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEKAKSIYLVSTCK 780
           LFKASLSLCKKKKH+RKK +SA  K  T  SLF RD +SSDFGPSTSEK+KS+Y +STCK
Sbjct: 721 LFKASLSLCKKKKHRRKKCRSAVFKCPTGGSLFSRDDMSSDFGPSTSEKSKSVYSISTCK 780

Query: 781 SRKKAKHG---------SKEEKQKEI-----------------SVLATKNELNCSTDFLK 840
           +R K+KHG          KE+ + E+                 S  AT N++NCSTD   
Sbjct: 781 ARNKSKHGFRDSNGSSARKEDLKVELPTDIVDKESGKRRTEAGSAPATMNQMNCSTD--- 840

Query: 841 RSEKEANHNKSIEANFSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGM 900
            S   ANHN SI+A + KDRK+S N+DGLH V T GFQN+VVEQW+GI MPSSEN F G 
Sbjct: 841 -SFIVANHNDSIKAIYPKDRKRSVNQDGLHHVHTNGFQNTVVEQWNGIDMPSSENGFTGT 900

Query: 901 ENISIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRSGSANE 938
           ENISIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIA KKSQLKKLKL+RSGSANE
Sbjct: 901 ENISIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIATKKSQLKKLKLERSGSANE 938

BLAST of Sgr020483 vs. NCBI nr
Match: XP_023537278.1 (ubiquitin carboxyl-terminal hydrolase 23 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1380.2 bits (3571), Expect = 0.0e+00
Identity = 743/964 (77.07%), Postives = 802/964 (83.20%), Query Frame = 0

Query: 1   MADGVVFKPK-HDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLN 60
           MADGVVFKP+  DN SDCPG S D L+ SLFQ+ +++ PARR+FKGFDNGGGDF L TLN
Sbjct: 1   MADGVVFKPELGDNQSDCPGASTD-LVFSLFQKRVDYVPARRTFKGFDNGGGDFELTTLN 60

Query: 61  PSSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGL 120
           P+SSF QKSGSNVDHSA KGKK+DGSELLENGLDPELSFEI+FRRI          GAGL
Sbjct: 61  PNSSFGQKSGSNVDHSALKGKKLDGSELLENGLDPELSFEITFRRI----------GAGL 120

Query: 121 QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI 180
           QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI
Sbjct: 121 QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI 180

Query: 181 LAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHK 240
           LAPKDLVSNLRCISRNF+NARQEDAHEYMVNLLESMHRCCLPLGLPSES SAYEKSLVHK
Sbjct: 181 LAPKDLVSNLRCISRNFKNARQEDAHEYMVNLLESMHRCCLPLGLPSESPSAYEKSLVHK 240

Query: 241 IFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQC 300
           IFGGRLRSQVKCMQCS+CSN FDPFLDLSLDIVKADSI+KAFKNFTTPELLDGGERQYQC
Sbjct: 241 IFGGRLRSQVKCMQCSFCSNKFDPFLDLSLDIVKADSIHKAFKNFTTPELLDGGERQYQC 300

Query: 301 QRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYAD 360
           QRCKQKVKALKQFTV+KAPYVLTIHLKRFQSYNLEEKIHKKIQF PTLDL PFVSGSYAD
Sbjct: 301 QRCKQKVKALKQFTVYKAPYVLTIHLKRFQSYNLEEKIHKKIQFSPTLDLAPFVSGSYAD 360

Query: 361 GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFY 420
           GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDR VFEQQAYMLFY
Sbjct: 361 GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRTVFEQQAYMLFY 420

Query: 421 VRDRRKVVLKKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKKLNGLSSDEL 480
           VRDRRKVV KK VD A KENMKTST LN+  SIVN G KD C QN TFEKKLNG S+DEL
Sbjct: 421 VRDRRKVVPKKPVDIASKENMKTSTNLNKPGSIVNPGQKDICTQNRTFEKKLNGPSNDEL 480

Query: 481 RKESKDSSNIDPSKTTVLNGPSVRIETEQASKECSVPATISMPISSLKRVLPLKTFGKDI 540
            KE KDSSN+  SKTT+ NGPSV  ETE+ASKECSVP   SMP S    +  LKTF K+I
Sbjct: 481 VKELKDSSNVGTSKTTISNGPSVPRETERASKECSVPEVSSMPKSPSVGISSLKTFNKEI 540

Query: 541 IPNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNLIDANT 600
           IPNSS A DLPSLPR MNS F ATNS+ SLAKAA+ EI+HV+R LGVSVAT L +IDAN 
Sbjct: 541 IPNSSSAGDLPSLPRGMNSSFHATNSKSSLAKAADTEINHVDRSLGVSVATSLRVIDANA 600

Query: 601 SAIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNSVGNLS 660
           SAI QA        ++ AAS QESGCKT +KSDPE++P QP+SE+SKV  S+Q S GNLS
Sbjct: 601 SAILQA-------NDNAAASIQESGCKTLKKSDPEVVPNQPMSESSKVGISDQISAGNLS 660

Query: 661 SEDASNCKKLVPDESNEISSSSTMTDGSLLTKTLDFKNGRRLKRKALKYHVGSLHLSSNI 720
           S DASNCKK+ PDESN+I SSSTM +G LL+KTLD K+GR LKRK LKY  GSLHLSSNI
Sbjct: 661 SGDASNCKKM-PDESNKI-SSSTMVEGLLLSKTLDSKHGRGLKRKLLKYPSGSLHLSSNI 720

Query: 721 LFKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEKAKSIYLVSTCK 780
           LFK SLSLCKKKKH+RKKRQS GSK LT ESLF R+ +SSDFGPSTSEK+KSI LVS  K
Sbjct: 721 LFKTSLSLCKKKKHRRKKRQSPGSKTLTVESLFSRNDVSSDFGPSTSEKSKSICLVS--K 780

Query: 781 SRKKAKHGSKE--------------------EKQKE------ISVLATKNELNCSTDFLK 840
           SRKK KHGS+E                    +K+ E       SV+AT N LNCSTD   
Sbjct: 781 SRKKDKHGSRESNGNCARKEGHKVESLTDIVDKEGEKRSTEAHSVVATMNTLNCSTD--- 840

Query: 841 RSEKEANHNKSIEANFSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGM 900
            S   ANHN S+E N  KDRK+S+N+DG    LT GFQ++VV+QWDGI MPSS N F GM
Sbjct: 841 -SIIVANHNGSVETNCLKDRKRSSNQDGSRRALTNGFQDTVVKQWDGIDMPSSGNGFTGM 900

Query: 901 ENISIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRSGSANE 938
           EN+SIGYVADEWDEEYDQGKRKKIRQFKH+F GPNPFQEIA KKSQ KK+K++R GS NE
Sbjct: 901 ENVSIGYVADEWDEEYDQGKRKKIRQFKHTFDGPNPFQEIATKKSQFKKVKVERCGSGNE 938

BLAST of Sgr020483 vs. ExPASy Swiss-Prot
Match: Q9FPS4 (Ubiquitin carboxyl-terminal hydrolase 23 OS=Arabidopsis thaliana OX=3702 GN=UBP23 PE=2 SV=2)

HSP 1 Score: 600.1 bits (1546), Expect = 4.2e-170
Identity = 399/940 (42.45%), Postives = 523/940 (55.64%), Query Frame = 0

Query: 9   PKHDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLNPSSSFAQKS 68
           P  +N   C    A    S++F R IEFHPAR+ F GF NG  DF++ETLNP SS  +  
Sbjct: 18  PSSNNNGSC--AVASSTASAVF-RKIEFHPARKPFNGFSNGRSDFKIETLNPCSSNQRLL 77

Query: 69  GSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGLQNLGNTCFL 128
                 SA   KK D S+LLE+G +P+L+F I+FR+I          GAGLQNLGNTCFL
Sbjct: 78  ------SAPSAKKPDSSDLLEHGFEPDLTFSITFRKI----------GAGLQNLGNTCFL 137

Query: 129 NSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRILAPKDLVSN 188
           NSVLQCLTYTEPLAA LQ+  HQ  CHVAGFCALCAIQKHV  A Q++GRILAPKDLVSN
Sbjct: 138 NSVLQCLTYTEPLAATLQTAAHQKYCHVAGFCALCAIQKHVRTARQANGRILAPKDLVSN 197

Query: 189 LRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHKIFGGRLRSQ 248
           LRCISRNFRN RQEDAHEYM+NLLE MH+C LP G+PSES+ AY +SLVHKIFGG LRSQ
Sbjct: 198 LRCISRNFRNCRQEDAHEYMINLLECMHKCSLPSGVPSESSDAYRRSLVHKIFGGSLRSQ 257

Query: 249 VKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKA 308
           VKC QCS+CSN FDPFLDLSLDI KADS+ +A   FT  ELLD G + YQC+RCKQKVKA
Sbjct: 258 VKCEQCSHCSNKFDPFLDLSLDISKADSLQRALSRFTAVELLDNGAKVYQCERCKQKVKA 317

Query: 309 LKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADGDLKYTLYG 368
            KQ TV KAPYVLT+HLKRF+++   EKI +K+ F   +D+KPFVSG + +G+LKYTLYG
Sbjct: 318 KKQLTVSKAPYVLTVHLKRFEAHR-SEKIDRKVDFTSAIDMKPFVSGPH-EGNLKYTLYG 377

Query: 369 VLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFYVRDRRKVVL 428
           VLVH+G S+ SGHY C+VRTSSGMWY+LDDNRV  V ++ VF Q+AYMLFYVRDR+  V 
Sbjct: 378 VLVHYGRSSHSGHYACFVRTSSGMWYSLDDNRVVQVSEKTVFNQKAYMLFYVRDRQNAVP 437

Query: 429 KKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKK-------LNGLSSDELRK 488
           K SV    KE+  T    NR + IV   +KD  V  ST  K+        NGL+      
Sbjct: 438 KNSVPVVKKESFAT----NRASLIVASNIKDQ-VNGSTVIKECGFGALVANGLA------ 497

Query: 489 ESKDSSNIDPSKTTVLNGPSVRIETEQ-------ASKECSVPATISMPISSLKRVLPLKT 548
                 +  PS   VL    +  +  Q        +KE       S P+ +     P   
Sbjct: 498 ---PLKSCGPSTPAVLTQKDLNAKETQNNAISNVEAKEILETENGSAPVKTCDLAAPTVL 557

Query: 549 FGKDIIPNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNL 608
             KD+       +++P LP+         N E SL K                       
Sbjct: 558 VQKDLNTKEIFQKEVP-LPQ--------ANGEGSLVKE---------------------- 617

Query: 609 IDANTSAIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNS 668
            D+  + +   E +  H+     A T         K++  +  E+  S N+  + +N   
Sbjct: 618 -DSKAACLILPEKVSPHLDGSANAQTLVKLPTLGPKAENSV--EEKNSLNNLNEPANSLK 677

Query: 669 VGNLSSEDASNCKKLVPDES--NEISSSSTMTDGSLLTKTLDF----KNGRRLKRKALKY 728
           V N+S  +    K ++ D++  + +  S+T  +   LT   +     K  R+ K K LK 
Sbjct: 678 VINVSVGNPPVEKAVLIDQTMGHHLEESATSIESLKLTSERETLTTPKKTRKPKTKTLKV 737

Query: 729 HVGSLHLSSNILFKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEK 788
                       FK +L L KKK  +R++  +  +  +  E L  +  +          K
Sbjct: 738 EFK--------FFKLALGLRKKKVQRRERLSTTVAGEIISEELLSKKRV----------K 797

Query: 789 AKSIYLVSTCKSRKKAKHGSKEEKQKEISVLATKNELNCSTDFLKRSEKEANHNKSIEAN 848
            +   L++  K    +      ++Q+ +           S+D  + S+      +S+   
Sbjct: 798 YQDTSLIAPSKMISSSDGAVTSDQQQPVG----------SSDLSEASQNAKRKRESVLL- 849

Query: 849 FSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGMENISIGYVADEWDEE 908
               +K++ N      +LT+G   +VV +WD     S +         SIGYVADEWDEE
Sbjct: 858 ----QKEAVN------ILTRGVPETVVAKWDEEISASQKRGSKSEGASSIGYVADEWDEE 849

Query: 909 YDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRS 929
           YD+GK+KKIR  + S+ GPNPFQ +A K+ +  K K  +S
Sbjct: 918 YDRGKKKKIRIKEESYRGPNPFQMLASKRQKETKKKWTQS 849

BLAST of Sgr020483 vs. ExPASy Swiss-Prot
Match: E1B9W9 (Ubiquitin carboxyl-terminal hydrolase 42 OS=Bos taurus OX=9913 GN=USP42 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 4.9e-78
Identity = 151/365 (41.37%), Postives = 225/365 (61.64%), Query Frame = 0

Query: 57  TLNPSSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKG 116
           +L P       S S+V   +    + D  + L +G+ P         +I      T   G
Sbjct: 53  SLGPVPGAVVYSSSSVPEKSKPSPQKD--QALGDGIAPPQKVLFPSEKICLKWQQTHRVG 112

Query: 117 AGLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSS 176
           AGLQNLGNTCF N+ LQCLTYT PLA Y+ S +H  +CH  GFC +C +Q H+++AL + 
Sbjct: 113 AGLQNLGNTCFANAALQCLTYTPPLANYMLSHEHSKTCHAEGFCMMCTMQAHITQALSNP 172

Query: 177 GRILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSL 236
           G ++ P  +++ +R I+R+FR   QEDAHE++   +++M + CL  G           +L
Sbjct: 173 GDVIKPMFVINEMRRIARHFRFGNQEDAHEFLQYTVDAMQKACLN-GSNKLDRHTQATTL 232

Query: 237 VHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQ 296
           V +IFGG LRS+VKC+ C   S+TFDP+LD++L+I  A S+ KA + F  PE LD GE  
Sbjct: 233 VCQIFGGYLRSRVKCLNCKGVSDTFDPYLDITLEIKAAQSVNKALEQFVKPEQLD-GENS 292

Query: 297 YQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGS 356
           Y+C +CK+ V A K+FT+H++  VLT+ LKRF ++    KI K +++   LD++P++S  
Sbjct: 293 YKCSKCKKMVPASKRFTIHRSSNVLTLSLKRFANFT-GGKIAKDVKYPEYLDIRPYMSQP 352

Query: 357 YADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYM 416
             +  + Y LY VLVH G +  +GHY+CY++ S+G+WY ++D+ VS    R V  QQAY+
Sbjct: 353 NGE-PIIYVLYAVLVHTGFNCHAGHYFCYIKASNGLWYQMNDSIVSTSDIRSVLSQQAYV 411

Query: 417 LFYVR 422
           LFY+R
Sbjct: 413 LFYIR 411

BLAST of Sgr020483 vs. ExPASy Swiss-Prot
Match: Q9H9J4 (Ubiquitin carboxyl-terminal hydrolase 42 OS=Homo sapiens OX=9606 GN=USP42 PE=1 SV=3)

HSP 1 Score: 294.3 bits (752), Expect = 4.9e-78
Identity = 151/365 (41.37%), Postives = 225/365 (61.64%), Query Frame = 0

Query: 57  TLNPSSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKG 116
           +L P       S S+V   +    + D  + L +G+ P         +I      T   G
Sbjct: 53  SLGPVPGAVVYSSSSVPDKSKPSPQKD--QALGDGIAPPQKVLFPSEKICLKWQQTHRVG 112

Query: 117 AGLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSS 176
           AGLQNLGNTCF N+ LQCLTYT PLA Y+ S +H  +CH  GFC +C +Q H+++AL + 
Sbjct: 113 AGLQNLGNTCFANAALQCLTYTPPLANYMLSHEHSKTCHAEGFCMMCTMQAHITQALSNP 172

Query: 177 GRILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSL 236
           G ++ P  +++ +R I+R+FR   QEDAHE++   +++M + CL  G           +L
Sbjct: 173 GDVIKPMFVINEMRRIARHFRFGNQEDAHEFLQYTVDAMQKACLN-GSNKLDRHTQATTL 232

Query: 237 VHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQ 296
           V +IFGG LRS+VKC+ C   S+TFDP+LD++L+I  A S+ KA + F  PE LD GE  
Sbjct: 233 VCQIFGGYLRSRVKCLNCKGVSDTFDPYLDITLEIKAAQSVNKALEQFVKPEQLD-GENS 292

Query: 297 YQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGS 356
           Y+C +CK+ V A K+FT+H++  VLT+ LKRF ++    KI K +++   LD++P++S  
Sbjct: 293 YKCSKCKKMVPASKRFTIHRSSNVLTLSLKRFANFT-GGKIAKDVKYPEYLDIRPYMSQP 352

Query: 357 YADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYM 416
             +  + Y LY VLVH G +  +GHY+CY++ S+G+WY ++D+ VS    R V  QQAY+
Sbjct: 353 NGE-PIVYVLYAVLVHTGFNCHAGHYFCYIKASNGLWYQMNDSIVSTSDIRSVLSQQAYV 411

Query: 417 LFYVR 422
           LFY+R
Sbjct: 413 LFYIR 411

BLAST of Sgr020483 vs. ExPASy Swiss-Prot
Match: B2RQC2 (Ubiquitin carboxyl-terminal hydrolase 42 OS=Mus musculus OX=10090 GN=Usp42 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 8.4e-78
Identity = 151/364 (41.48%), Postives = 224/364 (61.54%), Query Frame = 0

Query: 58  LNPSSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGA 117
           L P       S S+V   +      D  ++L +G+ P         +I      +   GA
Sbjct: 53  LGPVPGAVVYSNSSVPEKSKPSPPKD--QVLGDGIAPPQKVLFPSEKICLKWQQSHRVGA 112

Query: 118 GLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSG 177
           GLQNLGNTCF N+ LQCLTYT PLA Y+ S +H  +CH  GFC +C +Q H+++AL + G
Sbjct: 113 GLQNLGNTCFANAALQCLTYTPPLANYMLSHEHSKTCHAEGFCMMCTMQTHITQALSNPG 172

Query: 178 RILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLV 237
            ++ P  +++ +R I+R+FR   QEDAHE++   +++M + CL  G           +LV
Sbjct: 173 DVIKPMFVINEMRRIARHFRFGNQEDAHEFLQYTVDAMQKACLN-GSNKLDRHTQATTLV 232

Query: 238 HKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQY 297
            +IFGG LRS+VKC+ C   S+TFDP+LD++L+I  A S+ KA + F  PE LD GE  Y
Sbjct: 233 CQIFGGYLRSRVKCLNCKGVSDTFDPYLDITLEIKAAQSVTKALEQFVKPEQLD-GENSY 292

Query: 298 QCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSY 357
           +C +CK+ V A K+FT+H++  VLTI LKRF ++    KI K +++   LD++P++S   
Sbjct: 293 KCSKCKKMVPASKRFTIHRSSNVLTISLKRFANFT-GGKIAKDVKYPEYLDIRPYMSQPN 352

Query: 358 ADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYML 417
            +  + Y LY VLVH G +  +GHY+CY++ S+G+WY ++D+ VS    R V  QQAY+L
Sbjct: 353 GE-PIIYVLYAVLVHTGFNCHAGHYFCYIKASNGLWYQMNDSIVSTSDIRAVLNQQAYVL 410

Query: 418 FYVR 422
           FY+R
Sbjct: 413 FYIR 410

BLAST of Sgr020483 vs. ExPASy Swiss-Prot
Match: Q9P275 (Ubiquitin carboxyl-terminal hydrolase 36 OS=Homo sapiens OX=9606 GN=USP36 PE=1 SV=4)

HSP 1 Score: 293.1 bits (749), Expect = 1.1e-77
Identity = 192/538 (35.69%), Postives = 281/538 (52.23%), Query Frame = 0

Query: 32  RSIEFHPARRSFKGFDNGGGDFRLETLNPSSSFAQKSGSNVDHSASK-GKKM------DG 91
           + IEF PA +SF  +       +   LNP +  A +  S  D  A + G +       DG
Sbjct: 38  QKIEFEPASKSF-SYQLEALKSKYVLLNPKTEGASRHKSGDDPPARRQGSEHTYESCGDG 97

Query: 92  SELLENGLDPELSFEISFRRIVSVSSMTSVKGAGLQNLGNTCFLNSVLQCLTYTEPLAAY 151
               +  L P     + + R+  V       GAGL NLGNTCFLN+ +QCLTYT PLA Y
Sbjct: 98  VPAPQKVLFPTERLSLRWERVFRV-------GAGLHNLGNTCFLNATIQCLTYTPPLANY 157

Query: 152 LQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRILAPKDLVSNLRCISRNFRNARQEDA 211
           L S +H  SCH   FC LC +Q H+ +A  +SG  + P   + +L+ I+R+FR   QEDA
Sbjct: 158 LLSKEHARSCHQGSFCMLCVMQNHIVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDA 217

Query: 212 HEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHKIFGGRLRSQVKCMQCSYCSNTFDPF 271
           HE++   +++M + CL  G           +LVH+IFGG LRS+VKC  C   S+T+DP+
Sbjct: 218 HEFLRYTIDAMQKACLN-GCAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPY 277

Query: 272 LDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKALKQFTVHKAPYVLTIH 331
           LD++L+I +A +I +A + F   ++L  GE  Y C +CK+KV A K+FT+H+   VLT+ 
Sbjct: 278 LDVALEIRQAANIVRALELFVKADVL-SGENAYMCAKCKKKVPASKRFTIHRTSNVLTLS 337

Query: 332 LKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADGDLKYTLYGVLVHHGGSTRSGHYYC 391
           LKRF +++   KI K + +   L+++P++S +  D  + Y LY VLVH G S  +GHYYC
Sbjct: 338 LKRFANFS-GGKITKDVGYPEFLNIRPYMSQNNGD-PVMYGLYAVLVHSGYSCHAGHYYC 397

Query: 392 YVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFYVRDRRKVVLKKSVDGALKENMKTST 451
           YV+ S+G WY ++D+ V     ++V  QQAY+LFY+R       KKS +G +      S+
Sbjct: 398 YVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFYLRIPGS---KKSPEGLISRT--GSS 457

Query: 452 GLNRMASIVNQGLKDNCVQNSTFEKKLNGLSSDELRKESKDSSNIDPSKTTVLNGPSVRI 511
            L    S++    K N           NG+ S  L  + +DS  +    TT         
Sbjct: 458 SLPGRPSVIPDHSKKNIG---------NGIISSPLTGKRQDSGTMKKPHTT--------- 517

Query: 512 ETEQASKECSVPATISMPISSLKRVLPLKTFGKDI---IPNSSPAEDLPSLPRRMNSI 560
                         I +PIS     L LK+    I   +P+ SP+  L   P  M +I
Sbjct: 518 ------------EEIGVPISRNGSTLGLKSQNGCIPPKLPSGSPSPKLSQTPTHMPTI 528

BLAST of Sgr020483 vs. ExPASy TrEMBL
Match: A0A6J1E056 (Ubiquitin carboxyl-terminal hydrolase OS=Momordica charantia OX=3673 GN=LOC111024701 PE=3 SV=1)

HSP 1 Score: 1520.8 bits (3936), Expect = 0.0e+00
Identity = 796/964 (82.57%), Postives = 838/964 (86.93%), Query Frame = 0

Query: 1   MADGVVFKPKHDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLNP 60
           MADG VFKP+HDNPSDCPGTSAD LLSSLFQR ++FHPAR+SF GFDNGGGDFR ETLNP
Sbjct: 1   MADGFVFKPEHDNPSDCPGTSAD-LLSSLFQRRMDFHPARKSFNGFDNGGGDFRFETLNP 60

Query: 61  SSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGLQ 120
           SSS A KSGSNVDHSASKGKKMDGSELLENGLDPELSFEI+FRRI          GAGLQ
Sbjct: 61  SSSLAHKSGSNVDHSASKGKKMDGSELLENGLDPELSFEITFRRI----------GAGLQ 120

Query: 121 NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL 180
           NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL
Sbjct: 121 NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL 180

Query: 181 APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHKI 240
           APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSES SAYEKSLVHKI
Sbjct: 181 APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESPSAYEKSLVHKI 240

Query: 241 FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ 300
           FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ
Sbjct: 241 FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ 300

Query: 301 RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG 360
           RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG
Sbjct: 301 RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG 360

Query: 361 DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFYV 420
           DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDR VFEQQAYMLFYV
Sbjct: 361 DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRTVFEQQAYMLFYV 420

Query: 421 RDRRKVVLKKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKKLNGLSSDELR 480
           RDRRKVV KKS+D ALKENMK ST  NR  S+VNQGLKDNC QN TFEKKLNG +SDELR
Sbjct: 421 RDRRKVVQKKSIDIALKENMKMSTSSNRSDSVVNQGLKDNCAQNCTFEKKLNGPTSDELR 480

Query: 481 KESKDSSNIDPSKTTVLNGPSVRIETEQASKECSVPATISMPISSLKRVLPLKTFGKDII 540
           KESKDS N+ PSKT + NGPSV+IE EQAS ECSVP T+SMP  S + VLPLKTF +DI+
Sbjct: 481 KESKDSPNMGPSKTKISNGPSVQIEIEQASTECSVPVTVSMPTYSSRGVLPLKTFSEDIM 540

Query: 541 PNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNLIDANTS 600
           PNSSPAEDL SLP  MN+ F+ATNS  SLA+AA+IEI HVNRGLGV VAT LNLID NTS
Sbjct: 541 PNSSPAEDLTSLPVCMNNNFVATNSVSSLARAADIEIGHVNRGLGVLVATSLNLIDPNTS 600

Query: 601 AIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNSVGNLSS 660
           AIPQAELLVSHVQN  AA T+ESGCKT EKS PEILP+QP SE+SKVD  NQ  VGNLSS
Sbjct: 601 AIPQAELLVSHVQNGNAALTEESGCKTLEKSYPEILPDQPKSESSKVDIPNQKFVGNLSS 660

Query: 661 EDASNCKKLVPDESNEISSSSTMTDGSLLTKTLDFKNGRRLKRKALKYHVGSLHLSSNIL 720
           EDAS+CKKL+PDESN++S+SS MT   L+TKT D K+GRRLKRK LKYH+GSLHLSSNIL
Sbjct: 661 EDASDCKKLMPDESNKMSTSSIMTSELLVTKTPDSKHGRRLKRKNLKYHLGSLHLSSNIL 720

Query: 721 FKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEKAKSIYLVSTCKS 780
           FKASLSLCKK+KHKRKKRQSAGSKNLT+ESL   DGISSD GPSTSEKAKSIYLVSTCKS
Sbjct: 721 FKASLSLCKKRKHKRKKRQSAGSKNLTEESLSSTDGISSDLGPSTSEKAKSIYLVSTCKS 780

Query: 781 RKKAKHGSKE--------------------------EKQKEISVLATKNELNCSTDFLKR 840
           RKKAKHGS+E                            ++E S LATK +LNCS+D    
Sbjct: 781 RKKAKHGSREGNGISTKNEAHNVEELTDIVDKGSGKRSKEESSELATKKKLNCSSD---- 840

Query: 841 SEKEANHNKSIEANFSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGME 900
           S   ANH +SIE NF KDRK +AN+DG  CVLT GFQN+VVEQWDG KMPS EN F GME
Sbjct: 841 SIIVANHKESIEVNFPKDRKINANQDGFRCVLTNGFQNTVVEQWDGTKMPSFENGFSGME 900

Query: 901 NI-SIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRSGSANE 938
            I SIGYVADEWDEEYDQGKRKKIR+FKHSFGGPNPFQEIA KKSQ KKLKLDRSGSA E
Sbjct: 901 TIRSIGYVADEWDEEYDQGKRKKIREFKHSFGGPNPFQEIATKKSQFKKLKLDRSGSAKE 949

BLAST of Sgr020483 vs. ExPASy TrEMBL
Match: A0A6J1DWF7 (Ubiquitin carboxyl-terminal hydrolase OS=Momordica charantia OX=3673 GN=LOC111024701 PE=3 SV=1)

HSP 1 Score: 1498.8 bits (3879), Expect = 0.0e+00
Identity = 788/964 (81.74%), Postives = 830/964 (86.10%), Query Frame = 0

Query: 1   MADGVVFKPKHDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLNP 60
           MADG VFKP+HDNPSDCPGTSAD LLSSLFQR ++FHPAR+SF GFDNGGGDFR ETLNP
Sbjct: 1   MADGFVFKPEHDNPSDCPGTSAD-LLSSLFQRRMDFHPARKSFNGFDNGGGDFRFETLNP 60

Query: 61  SSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGLQ 120
           SSS A KSGSNVDHSASKGKKMDGSELLENGLDPELSFEI+FRRI          GAGLQ
Sbjct: 61  SSSLAHKSGSNVDHSASKGKKMDGSELLENGLDPELSFEITFRRI----------GAGLQ 120

Query: 121 NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL 180
           NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL
Sbjct: 121 NLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRIL 180

Query: 181 APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHKI 240
           APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSES SAYEKSLVHKI
Sbjct: 181 APKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESPSAYEKSLVHKI 240

Query: 241 FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ 300
           FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ
Sbjct: 241 FGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQ 300

Query: 301 RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG 360
           RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG
Sbjct: 301 RCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADG 360

Query: 361 DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFYV 420
           DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDR VFEQQAYMLFYV
Sbjct: 361 DLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRTVFEQQAYMLFYV 420

Query: 421 RDRRKVVLKKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKKLNGLSSDELR 480
           RDRRKVV KKS+D ALKENMK ST  NR  S+VNQGLKDNC QN TFEKKLNG +SDELR
Sbjct: 421 RDRRKVVQKKSIDIALKENMKMSTSSNRSDSVVNQGLKDNCAQNCTFEKKLNGPTSDELR 480

Query: 481 KESKDSSNIDPSKTTVLNGPSVRIETEQASKECSVPATISMPISSLKRVLPLKTFGKDII 540
           KESKDS N+ PSKT + NGPSV+IE EQAS ECSVP T+SMP  S + VLPLKTF +DI+
Sbjct: 481 KESKDSPNMGPSKTKISNGPSVQIEIEQASTECSVPVTVSMPTYSSRGVLPLKTFSEDIM 540

Query: 541 PNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNLIDANTS 600
           PNSSPAEDL SLP  MN+ F+ATNS  SLA+AA+IEI HVNRGLGV VAT LNLID NTS
Sbjct: 541 PNSSPAEDLTSLPVCMNNNFVATNSVSSLARAADIEIGHVNRGLGVLVATSLNLIDPNTS 600

Query: 601 AIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNSVGNLSS 660
           AIPQA        N  AA T+ESGCKT EKS PEILP+QP SE+SKVD  NQ  VGNLSS
Sbjct: 601 AIPQA--------NGNAALTEESGCKTLEKSYPEILPDQPKSESSKVDIPNQKFVGNLSS 660

Query: 661 EDASNCKKLVPDESNEISSSSTMTDGSLLTKTLDFKNGRRLKRKALKYHVGSLHLSSNIL 720
           EDAS+CKKL+PDESN++S+SS MT   L+TKT D K+GRRLKRK LKYH+GSLHLSSNIL
Sbjct: 661 EDASDCKKLMPDESNKMSTSSIMTSELLVTKTPDSKHGRRLKRKNLKYHLGSLHLSSNIL 720

Query: 721 FKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEKAKSIYLVSTCKS 780
           FKASLSLCKK+KHKRKKRQSAGSKNLT+ESL   DGISSD GPSTSEKAKSIYLVSTCKS
Sbjct: 721 FKASLSLCKKRKHKRKKRQSAGSKNLTEESLSSTDGISSDLGPSTSEKAKSIYLVSTCKS 780

Query: 781 RKKAKHGSKE--------------------------EKQKEISVLATKNELNCSTDFLKR 840
           RKKAKHGS+E                            ++E S LATK +LNCS+D    
Sbjct: 781 RKKAKHGSREGNGISTKNEAHNVEELTDIVDKGSGKRSKEESSELATKKKLNCSSD---- 840

Query: 841 SEKEANHNKSIEANFSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGME 900
           S   ANH +SIE NF KDRK +AN+DG  CVLT GFQN+VVEQWDG KMPS EN F GME
Sbjct: 841 SIIVANHKESIEVNFPKDRKINANQDGFRCVLTNGFQNTVVEQWDGTKMPSFENGFSGME 900

Query: 901 NI-SIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRSGSANE 938
            I SIGYVADEWDEEYDQGKRKKIR+FKHSFGGPNPFQEIA KKSQ KKLKLDRSGSA E
Sbjct: 901 TIRSIGYVADEWDEEYDQGKRKKIREFKHSFGGPNPFQEIATKKSQFKKLKLDRSGSAKE 941

BLAST of Sgr020483 vs. ExPASy TrEMBL
Match: A0A6J1DVA3 (Ubiquitin carboxyl-terminal hydrolase OS=Momordica charantia OX=3673 GN=LOC111024701 PE=3 SV=1)

HSP 1 Score: 1464.1 bits (3789), Expect = 0.0e+00
Identity = 767/931 (82.38%), Postives = 808/931 (86.79%), Query Frame = 0

Query: 34  IEFHPARRSFKGFDNGGGDFRLETLNPSSSFAQKSGSNVDHSASKGKKMDGSELLENGLD 93
           ++FHPAR+SF GFDNGGGDFR ETLNPSSS A KSGSNVDHSASKGKKMDGSELLENGLD
Sbjct: 1   MDFHPARKSFNGFDNGGGDFRFETLNPSSSLAHKSGSNVDHSASKGKKMDGSELLENGLD 60

Query: 94  PELSFEISFRRIVSVSSMTSVKGAGLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNS 153
           PELSFEI+FRRI          GAGLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNS
Sbjct: 61  PELSFEITFRRI----------GAGLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNS 120

Query: 154 CHVAGFCALCAIQKHVSRALQSSGRILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLE 213
           CHVAGFCALCAIQKHVSRALQSSGRILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLE
Sbjct: 121 CHVAGFCALCAIQKHVSRALQSSGRILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLE 180

Query: 214 SMHRCCLPLGLPSESASAYEKSLVHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVK 273
           SMHRCCLPLGLPSES SAYEKSLVHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVK
Sbjct: 181 SMHRCCLPLGLPSESPSAYEKSLVHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVK 240

Query: 274 ADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNL 333
           ADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNL
Sbjct: 241 ADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNL 300

Query: 334 EEKIHKKIQFGPTLDLKPFVSGSYADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMW 393
           EEKIHKKIQFGPTLDLKPFVSGSYADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMW
Sbjct: 301 EEKIHKKIQFGPTLDLKPFVSGSYADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMW 360

Query: 394 YALDDNRVSHVGDRIVFEQQAYMLFYVRDRRKVVLKKSVDGALKENMKTSTGLNRMASIV 453
           YALDDNRVSHVGDR VFEQQAYMLFYVRDRRKVV KKS+D ALKENMK ST  NR  S+V
Sbjct: 361 YALDDNRVSHVGDRTVFEQQAYMLFYVRDRRKVVQKKSIDIALKENMKMSTSSNRSDSVV 420

Query: 454 NQGLKDNCVQNSTFEKKLNGLSSDELRKESKDSSNIDPSKTTVLNGPSVRIETEQASKEC 513
           NQGLKDNC QN TFEKKLNG +SDELRKESKDS N+ PSKT + NGPSV+IE EQAS EC
Sbjct: 421 NQGLKDNCAQNCTFEKKLNGPTSDELRKESKDSPNMGPSKTKISNGPSVQIEIEQASTEC 480

Query: 514 SVPATISMPISSLKRVLPLKTFGKDIIPNSSPAEDLPSLPRRMNSIFLATNSECSLAKAA 573
           SVP T+SMP  S + VLPLKTF +DI+PNSSPAEDL SLP  MN+ F+ATNS  SLA+AA
Sbjct: 481 SVPVTVSMPTYSSRGVLPLKTFSEDIMPNSSPAEDLTSLPVCMNNNFVATNSVSSLARAA 540

Query: 574 NIEISHVNRGLGVSVATPLNLIDANTSAIPQAELLVSHVQNDTAASTQESGCKTSEKSDP 633
           +IEI HVNRGLGV VAT LNLID NTSAIPQAELLVSHVQN  AA T+ESGCKT EKS P
Sbjct: 541 DIEIGHVNRGLGVLVATSLNLIDPNTSAIPQAELLVSHVQNGNAALTEESGCKTLEKSYP 600

Query: 634 EILPEQPISENSKVDTSNQNSVGNLSSEDASNCKKLVPDESNEISSSSTMTDGSLLTKTL 693
           EILP+QP SE+SKVD  NQ  VGNLSSEDAS+CKKL+PDESN++S+SS MT   L+TKT 
Sbjct: 601 EILPDQPKSESSKVDIPNQKFVGNLSSEDASDCKKLMPDESNKMSTSSIMTSELLVTKTP 660

Query: 694 DFKNGRRLKRKALKYHVGSLHLSSNILFKASLSLCKKKKHKRKKRQSAGSKNLTDESLFG 753
           D K+GRRLKRK LKYH+GSLHLSSNILFKASLSLCKK+KHKRKKRQSAGSKNLT+ESL  
Sbjct: 661 DSKHGRRLKRKNLKYHLGSLHLSSNILFKASLSLCKKRKHKRKKRQSAGSKNLTEESLSS 720

Query: 754 RDGISSDFGPSTSEKAKSIYLVSTCKSRKKAKHGSKE----------------------- 813
            DGISSD GPSTSEKAKSIYLVSTCKSRKKAKHGS+E                       
Sbjct: 721 TDGISSDLGPSTSEKAKSIYLVSTCKSRKKAKHGSREGNGISTKNEAHNVEELTDIVDKG 780

Query: 814 ---EKQKEISVLATKNELNCSTDFLKRSEKEANHNKSIEANFSKDRKQSANRDGLHCVLT 873
                ++E S LATK +LNCS+D    S   ANH +SIE NF KDRK +AN+DG  CVLT
Sbjct: 781 SGKRSKEESSELATKKKLNCSSD----SIIVANHKESIEVNFPKDRKINANQDGFRCVLT 840

Query: 874 QGFQNSVVEQWDGIKMPSSENEFIGMENI-SIGYVADEWDEEYDQGKRKKIRQFKHSFGG 933
            GFQN+VVEQWDG KMPS EN F GME I SIGYVADEWDEEYDQGKRKKIR+FKHSFGG
Sbjct: 841 NGFQNTVVEQWDGTKMPSFENGFSGMETIRSIGYVADEWDEEYDQGKRKKIREFKHSFGG 900

Query: 934 PNPFQEIAMKKSQLKKLKLDRSGSANEPFRI 938
           PNPFQEIA KKSQ KKLKLDRSGSA EPFRI
Sbjct: 901 PNPFQEIATKKSQFKKLKLDRSGSAKEPFRI 917

BLAST of Sgr020483 vs. ExPASy TrEMBL
Match: A0A6J1I1H6 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468591 PE=3 SV=1)

HSP 1 Score: 1380.2 bits (3571), Expect = 0.0e+00
Identity = 744/964 (77.18%), Postives = 804/964 (83.40%), Query Frame = 0

Query: 1   MADGVVFKPK-HDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLN 60
           MADGVVFKP+  DN SDCPGTS D L+ SLFQ+ +++ PARR+FKGFDNGGGDF L TLN
Sbjct: 1   MADGVVFKPELGDNQSDCPGTSTD-LVFSLFQKRVDYVPARRTFKGFDNGGGDFELTTLN 60

Query: 61  PSSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGL 120
           P+SSF QKSGSNVDHSA KGKK+DGSELLENGLDPELSFEI+FRRI          GAGL
Sbjct: 61  PNSSFGQKSGSNVDHSALKGKKLDGSELLENGLDPELSFEITFRRI----------GAGL 120

Query: 121 QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI 180
           QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI
Sbjct: 121 QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI 180

Query: 181 LAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHK 240
           LAPKDLVSNLRCISRNF+NARQEDAHEYMVNLLESMHRCCLPLGLPSES SAYEKSLVHK
Sbjct: 181 LAPKDLVSNLRCISRNFKNARQEDAHEYMVNLLESMHRCCLPLGLPSESPSAYEKSLVHK 240

Query: 241 IFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQC 300
           IFGGRLRSQVKCMQCS+CSN FDPFLDLSLDIVKADSI+KAFKNFTTPELLDGGERQYQC
Sbjct: 241 IFGGRLRSQVKCMQCSFCSNKFDPFLDLSLDIVKADSIHKAFKNFTTPELLDGGERQYQC 300

Query: 301 QRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYAD 360
           QRCKQKVKALKQFTV+KAPYVLTIHLKRFQSYNLEEKIHKKIQF PTLDL PFVSGSYAD
Sbjct: 301 QRCKQKVKALKQFTVYKAPYVLTIHLKRFQSYNLEEKIHKKIQFSPTLDLAPFVSGSYAD 360

Query: 361 GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFY 420
           GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDR VFEQQAYMLFY
Sbjct: 361 GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRTVFEQQAYMLFY 420

Query: 421 VRDRRKVVLKKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKKLNGLSSDEL 480
           VRDRRKVV KK VD A KENMKTST LN+  SIVN G KDNC QN TFEKKLNG S+DEL
Sbjct: 421 VRDRRKVVPKKPVDIASKENMKTSTNLNKPGSIVNPGQKDNCTQNRTFEKKLNGPSNDEL 480

Query: 481 RKESKDSSNIDPSKTTVLNGPSVRIETEQASKECSVPATISMPISSLKRVLPLKTFGKDI 540
            KE KDSSN+  SKTT+ NGPSV  ETE+ASKECSV    SMP S    +  LKTF K+I
Sbjct: 481 VKELKDSSNVGTSKTTISNGPSVPRETERASKECSVLEASSMPKSPSVGISSLKTFNKEI 540

Query: 541 IPNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNLIDANT 600
           IPNSS AEDLP LPR MNS F ATNS+ SLAKAA+ EI+H +R LGVSVAT L++IDAN 
Sbjct: 541 IPNSSSAEDLPFLPRGMNSSFHATNSKSSLAKAADTEINHFDRSLGVSVATSLSVIDANA 600

Query: 601 SAIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNSVGNLS 660
           SAI QA        ND AAS QESGCKT +KSDPEI+P QPISE+SKV  S+Q SVGNLS
Sbjct: 601 SAILQA--------NDNAASIQESGCKTLKKSDPEIVPNQPISESSKVGISDQISVGNLS 660

Query: 661 SEDASNCKKLVPDESNEISSSSTMTDGSLLTKTLDFKNGRRLKRKALKYHVGSLHLSSNI 720
           S DASNCKK+ PD+SN+I SSSTM +G LL+KTLD K+GR LKRK LKY  GSLHLSSNI
Sbjct: 661 SADASNCKKM-PDKSNKI-SSSTMVEGLLLSKTLDSKHGRGLKRKLLKYPSGSLHLSSNI 720

Query: 721 LFKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEKAKSIYLVSTCK 780
           LFKASLSLCKKKKH+RKKRQS GSK+L  +SLF R+ +SSDFGPSTSEK++SI LVS  K
Sbjct: 721 LFKASLSLCKKKKHRRKKRQSPGSKSLAVKSLFSRNNVSSDFGPSTSEKSESICLVS--K 780

Query: 781 SRKKAKHGSKE--------------------EKQKE------ISVLATKNELNCSTDFLK 840
           SRKK KHGS+E                    +K+ E       SV+AT N LNCSTD   
Sbjct: 781 SRKKDKHGSRESNGNCARKEGHKVESLTDIVDKEGEKRSTEAHSVVATMNTLNCSTD--- 840

Query: 841 RSEKEANHNKSIEANFSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGM 900
            S   AN N S+E NF KDRK+++N+D     LT GFQ+SVV+QWDGI MPSS N F GM
Sbjct: 841 -SIIVANDNGSVETNFLKDRKRNSNQDDSRRALTNGFQDSVVKQWDGIDMPSSGNGFTGM 900

Query: 901 ENISIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRSGSANE 938
           EN+SIGYVADEWDEEYDQGKRKKIRQFKH+F GPNPFQEIA KKSQ KK+K++R GS NE
Sbjct: 901 ENVSIGYVADEWDEEYDQGKRKKIRQFKHTFDGPNPFQEIATKKSQFKKVKVERCGSGNE 937

BLAST of Sgr020483 vs. ExPASy TrEMBL
Match: A0A6J1FGV5 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111444069 PE=3 SV=1)

HSP 1 Score: 1377.5 bits (3564), Expect = 0.0e+00
Identity = 741/964 (76.87%), Postives = 804/964 (83.40%), Query Frame = 0

Query: 1   MADGVVFKPK-HDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLN 60
           MADGVVFKP+  DN SDCPGTS D L+ SLFQ+ +++ PARR+FKGFDNGGGDF L TLN
Sbjct: 1   MADGVVFKPELGDNQSDCPGTSTD-LVFSLFQKRVDYVPARRTFKGFDNGGGDFELTTLN 60

Query: 61  PSSSFAQKSGSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGL 120
           P+SSF QKSGSNVDHSA KGKK+DGSELLENGLDPELSFEI+FRRI          GAGL
Sbjct: 61  PNSSFGQKSGSNVDHSALKGKKLDGSELLENGLDPELSFEITFRRI----------GAGL 120

Query: 121 QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI 180
           QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI
Sbjct: 121 QNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRI 180

Query: 181 LAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHK 240
           LAPKDLVSNLRCISRNF+NARQEDAHEYMVNLLESMHRCCLPLGLPSES SAYEKSLVHK
Sbjct: 181 LAPKDLVSNLRCISRNFKNARQEDAHEYMVNLLESMHRCCLPLGLPSESPSAYEKSLVHK 240

Query: 241 IFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQC 300
           IFGGRLRSQVKCMQCS+CSN FDPFLDLSLDIVKADSI+KAFKNFTTPELLDGGERQYQC
Sbjct: 241 IFGGRLRSQVKCMQCSFCSNKFDPFLDLSLDIVKADSIHKAFKNFTTPELLDGGERQYQC 300

Query: 301 QRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYAD 360
           QRCKQKVKALKQFTV+KAPYVLTIHLKRFQSYNLEEKIHKKIQF PTLDL PFVSGSYAD
Sbjct: 301 QRCKQKVKALKQFTVYKAPYVLTIHLKRFQSYNLEEKIHKKIQFSPTLDLAPFVSGSYAD 360

Query: 361 GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFY 420
           GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDR VFEQQAYMLFY
Sbjct: 361 GDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRTVFEQQAYMLFY 420

Query: 421 VRDRRKVVLKKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKKLNGLSSDEL 480
           VRDRRKVV KK VD A KENMKTST LN+  SIVN G K NC QN TFEKKLNG S+DEL
Sbjct: 421 VRDRRKVVPKKPVDIASKENMKTSTNLNKPGSIVNPGQKVNCTQNRTFEKKLNGPSNDEL 480

Query: 481 RKESKDSSNIDPSKTTVLNGPSVRIETEQASKECSVPATISMPISSLKRVLPLKTFGKDI 540
            KE KDSSN+  SKTT+ NGPSV  ETE+ASKECSVP   SMP S    +  LKTF K+I
Sbjct: 481 VKELKDSSNVGTSKTTISNGPSVPRETERASKECSVPEASSMPKSPSLGISSLKTFNKEI 540

Query: 541 IPNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNLIDANT 600
           IPN S AEDLPSLPR MNS F ATNS+ SLAKAA+ EI+HV+R LGVSVAT L++IDAN 
Sbjct: 541 IPNPSSAEDLPSLPRGMNSSFHATNSKSSLAKAADSEINHVDRSLGVSVATSLSVIDANA 600

Query: 601 SAIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNSVGNLS 660
           SAI QA        ++ AAS  ESGCKT +KSDPE++P QP+SE+SKV  S+Q S GNLS
Sbjct: 601 SAILQA-------NDNAAASILESGCKTLKKSDPEVVPNQPMSESSKVGISDQISAGNLS 660

Query: 661 SEDASNCKKLVPDESNEISSSSTMTDGSLLTKTLDFKNGRRLKRKALKYHVGSLHLSSNI 720
           S DASNCKK+ PDESN+I SSSTM +G LL+KTLD K+GR LKRK LKY  GSLHLSSNI
Sbjct: 661 SGDASNCKKM-PDESNKI-SSSTMLEGLLLSKTLDSKHGRGLKRKLLKYPSGSLHLSSNI 720

Query: 721 LFKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEKAKSIYLVSTCK 780
           LFK SLSLCKKKKH+RKKRQS GSK+LT ESLF R+ +SSDFGPSTSEK+KSI LVS  K
Sbjct: 721 LFKTSLSLCKKKKHRRKKRQSPGSKSLTVESLFSRNDLSSDFGPSTSEKSKSICLVS--K 780

Query: 781 SRKKAKHGSK-------------------------EEKQKEI-SVLATKNELNCSTDFLK 840
           SRKK KHGS+                         E++  E+ SV+AT N LNCST    
Sbjct: 781 SRKKDKHGSRESNGNCARKEGHKVESVTNIVDKEGEKRSTEVHSVVATMNTLNCST---- 840

Query: 841 RSEKEANHNKSIEANFSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGM 900
            S   ANHN+S+E N  KDRK+S+N+DG    LT GFQ++VV+QWDGI MPSS N F GM
Sbjct: 841 HSIIVANHNESVETNGLKDRKRSSNQDGSCRALTNGFQDTVVKQWDGIDMPSSGNGFTGM 900

Query: 901 ENISIGYVADEWDEEYDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRSGSANE 938
           EN+SIGYVADEWDEEYDQGKRKKIRQFKH+F GPNPFQEIA KKSQ KK+K++R GS NE
Sbjct: 901 ENVSIGYVADEWDEEYDQGKRKKIRQFKHTFDGPNPFQEIATKKSQFKKVKVERCGSGNE 938

BLAST of Sgr020483 vs. TAIR 10
Match: AT5G57990.1 (ubiquitin-specific protease 23 )

HSP 1 Score: 600.1 bits (1546), Expect = 3.0e-171
Identity = 399/940 (42.45%), Postives = 523/940 (55.64%), Query Frame = 0

Query: 9   PKHDNPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLNPSSSFAQKS 68
           P  +N   C    A    S++F R IEFHPAR+ F GF NG  DF++ETLNP SS  +  
Sbjct: 18  PSSNNNGSC--AVASSTASAVF-RKIEFHPARKPFNGFSNGRSDFKIETLNPCSSNQRLL 77

Query: 69  GSNVDHSASKGKKMDGSELLENGLDPELSFEISFRRIVSVSSMTSVKGAGLQNLGNTCFL 128
                 SA   KK D S+LLE+G +P+L+F I+FR+I          GAGLQNLGNTCFL
Sbjct: 78  ------SAPSAKKPDSSDLLEHGFEPDLTFSITFRKI----------GAGLQNLGNTCFL 137

Query: 129 NSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSGRILAPKDLVSN 188
           NSVLQCLTYTEPLAA LQ+  HQ  CHVAGFCALCAIQKHV  A Q++GRILAPKDLVSN
Sbjct: 138 NSVLQCLTYTEPLAATLQTAAHQKYCHVAGFCALCAIQKHVRTARQANGRILAPKDLVSN 197

Query: 189 LRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHKIFGGRLRSQ 248
           LRCISRNFRN RQEDAHEYM+NLLE MH+C LP G+PSES+ AY +SLVHKIFGG LRSQ
Sbjct: 198 LRCISRNFRNCRQEDAHEYMINLLECMHKCSLPSGVPSESSDAYRRSLVHKIFGGSLRSQ 257

Query: 249 VKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKA 308
           VKC QCS+CSN FDPFLDLSLDI KADS+ +A   FT  ELLD G + YQC+RCKQKVKA
Sbjct: 258 VKCEQCSHCSNKFDPFLDLSLDISKADSLQRALSRFTAVELLDNGAKVYQCERCKQKVKA 317

Query: 309 LKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVSGSYADGDLKYTLYG 368
            KQ TV KAPYVLT+HLKRF+++   EKI +K+ F   +D+KPFVSG + +G+LKYTLYG
Sbjct: 318 KKQLTVSKAPYVLTVHLKRFEAHR-SEKIDRKVDFTSAIDMKPFVSGPH-EGNLKYTLYG 377

Query: 369 VLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFYVRDRRKVVL 428
           VLVH+G S+ SGHY C+VRTSSGMWY+LDDNRV  V ++ VF Q+AYMLFYVRDR+  V 
Sbjct: 378 VLVHYGRSSHSGHYACFVRTSSGMWYSLDDNRVVQVSEKTVFNQKAYMLFYVRDRQNAVP 437

Query: 429 KKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEKK-------LNGLSSDELRK 488
           K SV    KE+  T    NR + IV   +KD  V  ST  K+        NGL+      
Sbjct: 438 KNSVPVVKKESFAT----NRASLIVASNIKDQ-VNGSTVIKECGFGALVANGLA------ 497

Query: 489 ESKDSSNIDPSKTTVLNGPSVRIETEQ-------ASKECSVPATISMPISSLKRVLPLKT 548
                 +  PS   VL    +  +  Q        +KE       S P+ +     P   
Sbjct: 498 ---PLKSCGPSTPAVLTQKDLNAKETQNNAISNVEAKEILETENGSAPVKTCDLAAPTVL 557

Query: 549 FGKDIIPNSSPAEDLPSLPRRMNSIFLATNSECSLAKAANIEISHVNRGLGVSVATPLNL 608
             KD+       +++P LP+         N E SL K                       
Sbjct: 558 VQKDLNTKEIFQKEVP-LPQ--------ANGEGSLVKE---------------------- 617

Query: 609 IDANTSAIPQAELLVSHVQNDTAASTQESGCKTSEKSDPEILPEQPISENSKVDTSNQNS 668
            D+  + +   E +  H+     A T         K++  +  E+  S N+  + +N   
Sbjct: 618 -DSKAACLILPEKVSPHLDGSANAQTLVKLPTLGPKAENSV--EEKNSLNNLNEPANSLK 677

Query: 669 VGNLSSEDASNCKKLVPDES--NEISSSSTMTDGSLLTKTLDF----KNGRRLKRKALKY 728
           V N+S  +    K ++ D++  + +  S+T  +   LT   +     K  R+ K K LK 
Sbjct: 678 VINVSVGNPPVEKAVLIDQTMGHHLEESATSIESLKLTSERETLTTPKKTRKPKTKTLKV 737

Query: 729 HVGSLHLSSNILFKASLSLCKKKKHKRKKRQSAGSKNLTDESLFGRDGISSDFGPSTSEK 788
                       FK +L L KKK  +R++  +  +  +  E L  +  +          K
Sbjct: 738 EFK--------FFKLALGLRKKKVQRRERLSTTVAGEIISEELLSKKRV----------K 797

Query: 789 AKSIYLVSTCKSRKKAKHGSKEEKQKEISVLATKNELNCSTDFLKRSEKEANHNKSIEAN 848
            +   L++  K    +      ++Q+ +           S+D  + S+      +S+   
Sbjct: 798 YQDTSLIAPSKMISSSDGAVTSDQQQPVG----------SSDLSEASQNAKRKRESVLL- 849

Query: 849 FSKDRKQSANRDGLHCVLTQGFQNSVVEQWDGIKMPSSENEFIGMENISIGYVADEWDEE 908
               +K++ N      +LT+G   +VV +WD     S +         SIGYVADEWDEE
Sbjct: 858 ----QKEAVN------ILTRGVPETVVAKWDEEISASQKRGSKSEGASSIGYVADEWDEE 849

Query: 909 YDQGKRKKIRQFKHSFGGPNPFQEIAMKKSQLKKLKLDRS 929
           YD+GK+KKIR  + S+ GPNPFQ +A K+ +  K K  +S
Sbjct: 918 YDRGKKKKIRIKEESYRGPNPFQMLASKRQKETKKKWTQS 849

BLAST of Sgr020483 vs. TAIR 10
Match: AT3G14400.1 (ubiquitin-specific protease 25 )

HSP 1 Score: 229.9 bits (585), Expect = 8.1e-60
Identity = 159/466 (34.12%), Postives = 247/466 (53.00%), Query Frame = 0

Query: 118 GLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSC--HVAG----FCALCAIQKHVSR 177
           GL+NLGNTC+LNSVLQCLT+T PLA +  + KH + C  +V G     C  C ++K ++R
Sbjct: 25  GLRNLGNTCYLNSVLQCLTFTPPLANFCLTHKHSSHCDTYVDGERKRDCPFCIVEKRIAR 84

Query: 178 ALQSSGRILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASA 237
           +L       AP  + S L+  + +F+  RQEDAHE++  ++++ H   L L     + + 
Sbjct: 85  SLSVDLTTDAPNKISSCLKIFAEHFKLGRQEDAHEFLRYVIDACHNTSLRLKKLRYNGNE 144

Query: 238 --YEKSLVHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPEL 297
                S+V +IFGG L+SQVKC+ C   SN  D  +D+SL+I+++ S+ ++ + F   E+
Sbjct: 145 PFNGNSVVKEIFGGALQSQVKCLSCGAESNKADEIMDISLEILQSSSVKESLQKFFQSEI 204

Query: 298 LDGGERQYQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDL 357
           LDG  + Y+C+ C++ V A KQ ++ +AP +L I LKRF       KI K I FG  L L
Sbjct: 205 LDGNNK-YRCESCEKLVTARKQMSILQAPNILVIQLKRFGGI-FGGKIDKAISFGEILVL 264

Query: 358 KPFVSGSYADGDLKYTLYGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIV 417
             F+S +  D   +Y L+G++VH G S  SGHYY YV+ S G WY  +D+ VS    + V
Sbjct: 265 SNFMSKASKDPQPEYKLFGIIVHSGFSPESGHYYAYVKDSLGRWYCCNDSFVSLSTLQEV 324

Query: 418 FEQQAYMLFYVRDRRKVVLKKSVDGALKENMKTSTGLNRMASIVNQGLKDNCVQNSTFEK 477
             ++AY+LF+ R  ++    K++   +  N  TS  +N        G + +  Q   F  
Sbjct: 325 LSEKAYILFFSRSNQRPASAKTL---VTSNGTTSHEVN--------GCETSNPQK--FIG 384

Query: 478 KLNGLSSDELRKESKDSSNIDPSKTTVLNGPSVRIE------------TEQASKECSVPA 537
            LNG +     ++S    N+  SK      P  R E             E+A        
Sbjct: 385 PLNGFNMKPQAEQSFQKGNLASSKPHKFIRPKPRAEQAPLEDNLLSSKVEKAPLRPHAKV 444

Query: 538 TISMPISSLKRVLPLK---TFGKD--IIPNSSPAEDLPSLPRRMNS 559
           +IS+ + + KRV P+    +F +D  I P ++    +  LP ++NS
Sbjct: 445 SISVNLGA-KRVSPVNGRLSFHQDENIAPKANKENSVSVLPTKVNS 474

BLAST of Sgr020483 vs. TAIR 10
Match: AT5G46740.1 (ubiquitin-specific protease 21 )

HSP 1 Score: 218.8 bits (556), Expect = 1.9e-56
Identity = 148/416 (35.58%), Postives = 215/416 (51.68%), Query Frame = 0

Query: 13  NPSDCPGTSADELLSSLFQRSIEFHPARRSFKGFDNGGGDFRLETLNPSSSFAQKSGSNV 72
           NP +    + DE  SS   +++   PA+ S    +N      L   N S S+  +     
Sbjct: 74  NPEENRDVNPDESSSSPSDKTLIAPPAQISPVSNNN-----HLRITNTSDSYLYRPPRRY 133

Query: 73  DHSASKGKKMDGSELLENGLDPELSFEIS--FRRIVSVSSMTSVKGAGLQNLGNTCFLNS 132
               S     D  EL  N ++P    ++S  + RI      T V GAGL N GNTCF+ S
Sbjct: 134 IEYES-----DDDEL--NKMEPTKPLQLSWWYPRI----EPTGV-GAGLYNSGNTCFIAS 193

Query: 133 VLQCLTYTEPLAAYLQSGKHQNSCHVAG--FCALCAIQKHVSRALQSSGRILAPKDLVSN 192
           VLQC T+T PL   L+S  + N C+     FC + A++ H+  AL+SSG  +       N
Sbjct: 194 VLQCFTHTVPLIDSLRSFMYGNPCNCGNEKFCVMQALRDHIELALRSSGYGINIDRFRDN 253

Query: 193 LRCISRNFRNARQEDAHEYMVNLLESMHRCCLPLGLPSESASAYEKSLVHKIFGGRLRSQ 252
           L   S +F    QEDAHE++ + L+ + RCCL       S S+ + ++V  +FGG L S 
Sbjct: 254 LTYFSSDFMINHQEDAHEFLQSFLDKLERCCLDPKNQLGSVSSQDLNIVDNVFGGGLMST 313

Query: 253 VKCMQCSYCSNTFDPFLDLSLDIVKADSIYKAFKNFTTPELLDGGERQYQCQRCKQKVKA 312
           + C  C+  SNTF+P L  SL+I   ++++KA ++FT  E L   E Q  C  CK+KV  
Sbjct: 314 LCCCNCNSVSNTFEPSLGWSLEIEDVNTLWKALESFTCVEKL---EDQLTCDNCKEKVTK 373

Query: 313 LKQFTVHKAPYVLTIHLKRFQSYNLE-EKIHKKIQFGPTLDLKPFVSGSY-ADGDLKYTL 372
            KQ    K P V T HLKRF +  +  EKI   I+F   LDL PF+S ++  +   +Y L
Sbjct: 374 EKQLRFDKLPPVATFHLKRFTNDGVTMEKIFDHIEFPLELDLSPFMSSNHDPEVSTRYHL 433

Query: 373 YGVLVHHGGSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQQAYMLFYVRD 423
           Y  + H G     GHY  YVR++   W+  DD++V+ + +  V  + AY+LFY R+
Sbjct: 434 YAFVEHIGIRATFGHYSSYVRSAPETWHNFDDSKVTRISEERVLSRPAYILFYARE 469

BLAST of Sgr020483 vs. TAIR 10
Match: AT1G17110.1 (ubiquitin-specific protease 15 )

HSP 1 Score: 218.0 bits (554), Expect = 3.2e-56
Identity = 127/309 (41.10%), Postives = 181/309 (58.58%), Query Frame = 0

Query: 118 GLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSG 177
           GL N GN+C+ N+VLQ LT T+PL AYL    H  SC    +C +C +++HV   L+ SG
Sbjct: 439 GLVNCGNSCYANAVLQSLTCTKPLVAYLLRRSHSRSCSGKDWCLMCELEQHV-MMLRESG 498

Query: 178 RILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPL--GLPSESASAYEKS 237
             L+   ++S++R I+    +  QEDAHE++  L+ SM   CL    G         E +
Sbjct: 499 GPLSASRILSHMRSINCQIGDGSQEDAHEFLRLLVASMQSICLERLGGETKVDPRLQETT 558

Query: 238 LVHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVK-ADSIYKAFKNFTTPELLDGGE 297
           LV  +FGGRLRS+VKC++C + S  ++  +DL+L+I    +S+  A   FT PE LD GE
Sbjct: 559 LVQHMFGGRLRSKVKCLRCDHESERYENIMDLTLEIYGWVESLQDALTQFTRPEDLD-GE 618

Query: 298 RQYQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVS 357
             Y+C RC   V+A K+ ++H+AP +LTI LKRFQ      KI+K I F   LD+ PF++
Sbjct: 619 NMYRCSRCAGYVRARKELSIHEAPNILTIVLKRFQE-GRYGKINKCISFPEMLDMIPFMT 678

Query: 358 GSYADGDLKYTLYGVLVHHG--GSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQ 417
            +  D    Y LY V+VH     ++ SGHY  YV+   G WY +DD+ +  V    V  +
Sbjct: 679 RT-GDVPPLYMLYAVIVHLDTLNASFSGHYISYVKDLRGNWYRIDDSEIHPVPMTQVMSE 738

Query: 418 QAYMLFYVR 422
            AYMLFY+R
Sbjct: 739 GAYMLFYMR 743

BLAST of Sgr020483 vs. TAIR 10
Match: AT1G17110.2 (ubiquitin-specific protease 15 )

HSP 1 Score: 218.0 bits (554), Expect = 3.2e-56
Identity = 127/309 (41.10%), Postives = 181/309 (58.58%), Query Frame = 0

Query: 118 GLQNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQNSCHVAGFCALCAIQKHVSRALQSSG 177
           GL N GN+C+ N+VLQ LT T+PL AYL    H  SC    +C +C +++HV   L+ SG
Sbjct: 443 GLVNCGNSCYANAVLQSLTCTKPLVAYLLRRSHSRSCSGKDWCLMCELEQHV-MMLRESG 502

Query: 178 RILAPKDLVSNLRCISRNFRNARQEDAHEYMVNLLESMHRCCLPL--GLPSESASAYEKS 237
             L+   ++S++R I+    +  QEDAHE++  L+ SM   CL    G         E +
Sbjct: 503 GPLSASRILSHMRSINCQIGDGSQEDAHEFLRLLVASMQSICLERLGGETKVDPRLQETT 562

Query: 238 LVHKIFGGRLRSQVKCMQCSYCSNTFDPFLDLSLDIVK-ADSIYKAFKNFTTPELLDGGE 297
           LV  +FGGRLRS+VKC++C + S  ++  +DL+L+I    +S+  A   FT PE LD GE
Sbjct: 563 LVQHMFGGRLRSKVKCLRCDHESERYENIMDLTLEIYGWVESLQDALTQFTRPEDLD-GE 622

Query: 298 RQYQCQRCKQKVKALKQFTVHKAPYVLTIHLKRFQSYNLEEKIHKKIQFGPTLDLKPFVS 357
             Y+C RC   V+A K+ ++H+AP +LTI LKRFQ      KI+K I F   LD+ PF++
Sbjct: 623 NMYRCSRCAGYVRARKELSIHEAPNILTIVLKRFQE-GRYGKINKCISFPEMLDMIPFMT 682

Query: 358 GSYADGDLKYTLYGVLVHHG--GSTRSGHYYCYVRTSSGMWYALDDNRVSHVGDRIVFEQ 417
            +  D    Y LY V+VH     ++ SGHY  YV+   G WY +DD+ +  V    V  +
Sbjct: 683 RT-GDVPPLYMLYAVIVHLDTLNASFSGHYISYVKDLRGNWYRIDDSEIHPVPMTQVMSE 742

Query: 418 QAYMLFYVR 422
            AYMLFY+R
Sbjct: 743 GAYMLFYMR 747