Sgr019899 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr019899 vs. NCBI nr
Match: XP_022158085.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Momordica charantia])

HSP 1 Score: 3066.6 bits (7949), Expect = 0.0e+00
Identity = 1542/1799 (85.71%), Postives = 1622/1799 (90.16%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPW+ DPANVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCSKCTANFIPAP ID+  AR+DS+KIRVCN+CFKQWGKEPS+ Q+ DNA SSA+S
Sbjct: 61   CGRVFCSKCTANFIPAPPIDTANARDDSDKIRVCNFCFKQWGKEPSAAQDGDNATSSALS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
             S SS+S+SSTKSGYTCHSAISNIASTPCS+  RY+DP+CSG I+DEQD+ +NGK INR 
Sbjct: 121  LSSSSSSMSSTKSGYTCHSAISNIASTPCSTDRRYYDPFCSGGISDEQDSSINGKSINRA 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTD-IDHIAVQGL 240
            +S+MT+SSS+YGYYRSDDEDDDYYYG+YRSDSESRHISQSDDYY AVN D +D IA Q L
Sbjct: 181  SSMMTTSSSFYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNPDEMDRIAAQSL 240

Query: 241  NLDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNN 300
            NLDREN STKGS  IF+ Q PG +E+SSEDSDENDKGSECDFPTY MQNKDNEP+DFQNN
Sbjct: 241  NLDRENTSTKGSPEIFETQRPGINEKSSEDSDENDKGSECDFPTYAMQNKDNEPVDFQNN 300

Query: 301  GLLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKN 360
            G+LWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSS   GE PM GR +EEY+KTAKN
Sbjct: 301  GILWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSLAGGECPMKGRSSEEYRKTAKN 360

Query: 361  VVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGY 420
            VVEGHFRALVGQLLEAENLP+GDE +KDGWLDIITYLSWEAA VLKPDTSKSGGMDPGGY
Sbjct: 361  VVEGHFRALVGQLLEAENLPLGDEPDKDGWLDIITYLSWEAATVLKPDTSKSGGMDPGGY 420

Query: 421  VKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFD 480
            VKVKCVAGGK SESIVVKGVICKKNVAHRRMTR ISKPRFF+LGGALEYQRV+NHLSSFD
Sbjct: 421  VKVKCVAGGKCSESIVVKGVICKKNVAHRRMTRTISKPRFFVLGGALEYQRVTNHLSSFD 480

Query: 481  TLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIAC 540
            TLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIAC
Sbjct: 481  TLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIAC 540

Query: 541  CTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCC 600
            CTGANI+PSVDHVASLKLGYCDAFRVETFVEEHDG DGQA KK  KTLMFFEGCPKPLCC
Sbjct: 541  CTGANIVPSVDHVASLKLGYCDAFRVETFVEEHDGVDGQAGKKFAKTLMFFEGCPKPLCC 600

Query: 601  TILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNP 660
            TILLRGAS DELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+KSPITVALPHNP
Sbjct: 601  TILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPITVALPHNP 660

Query: 661  SNIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTL 720
            S+IDRSISAIPGFTGP P K                     +SSI   NV+K +  EPTL
Sbjct: 661  SSIDRSISAIPGFTGPSPAKSPEPEPSMEIWQCGKDAVLTRQSSIGCPNVIKSNSLEPTL 720

Query: 721  SQLKYNATSTSSSFLKHDVSTAYHYMLSSKFASEASRSLDIKECFMGMTTIKEEAGVDDD 780
              LK NATSTS S    DVS AY+YMLSSKFASEASRSLDIKEC MGM  IKEEAG DDD
Sbjct: 721  RPLKSNATSTSLSSFDQDVSIAYNYMLSSKFASEASRSLDIKECSMGMMAIKEEAGADDD 780

Query: 781  TISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKANLVTKSSREDFPLS 840
            T    SGIGE LSQ GNS YISSNSLDG   S+IDLA L+PEST +NLVTKSSREDFPLS
Sbjct: 781  T---SSGIGETLSQGGNSSYISSNSLDGTIQSYIDLACLKPESTSSNLVTKSSREDFPLS 840

Query: 841  PSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCGM 900
            PSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCGM
Sbjct: 841  PSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCGM 900

Query: 901  PSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIM 960
            PSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIM
Sbjct: 901  PSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIM 960

Query: 961  SDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSV 1020
            SDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSV
Sbjct: 961  SDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSV 1020

Query: 1021 HIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNELK 1080
            H+PPS LEF+HNN DWI KEA EVHNRA+ +FA VC ALPQI KNESG EQEDLGVNEL 
Sbjct: 1021 HLPPSILEFYHNNLDWINKEATEVHNRAEIIFAGVCKALPQISKNESGIEQEDLGVNELN 1080

Query: 1081 HYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRLS 1140
             YIQELEQI +MEKEDFEVH KEVLSGET V QPAVDILEINKLHMHVL+LSYIWVQRLS
Sbjct: 1081 QYIQELEQIISMEKEDFEVHLKEVLSGETRVYQPAVDILEINKLHMHVLYLSYIWVQRLS 1140

Query: 1141 FAATLRNHNSLDSLSNDIPTLEERRLDSVEELV-ELNLASRQDEFFSGCDNDSLPSDSKH 1200
            FAATLRN NS DSLSNDIP     RLDSVEELV  LNLAS QDEFF GCDNDSLPSDSKH
Sbjct: 1141 FAATLRNKNSQDSLSNDIP-----RLDSVEELVAALNLASGQDEFFVGCDNDSLPSDSKH 1200

Query: 1201 EIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
            EIDLGKGGNS QINQSNEME EK++DLGGQEDE CLS V+SEPL VGITI +A+S+GEYP
Sbjct: 1201 EIDLGKGGNSVQINQSNEMESEKHMDLGGQEDEFCLSRVDSEPLGVGITIERAISVGEYP 1260

Query: 1261 IMDNLSGTLDAVWKAETHPNSVTSEESG-SQTNSAILEPLAGHSDFEKCIANLVGVEAAQ 1320
            IM +LS TLDAVWK   HPNS TSEES  S+TNS ILEPLAG SD EKC ANLVGVEAAQ
Sbjct: 1261 IMSDLSDTLDAVWKGAVHPNSGTSEESNDSRTNSGILEPLAGQSDMEKCTANLVGVEAAQ 1320

Query: 1321 LLHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQG 1380
            LLHSALVLR VD++ET   SMSM SS YN+KISLLNVPKPE +DYDPAFISSFRELEKQG
Sbjct: 1321 LLHSALVLRSVDSIET---SMSMPSSAYNTKISLLNVPKPEFSDYDPAFISSFRELEKQG 1380

Query: 1381 HLRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDS 1440
            HLR LM VG NDT+IPVYDDEPTSI+AYSLV+PEYHAQMSEPERV+EAGDT RS P  DS
Sbjct: 1381 HLRFLMHVGVNDTIIPVYDDEPTSIVAYSLVIPEYHAQMSEPERVMEAGDTARSLPLLDS 1440

Query: 1441 ANLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPL 1500
            A   SL SF   VS+ YKS SSNEE+ L   R+QSIQFE+LLLYTKDLHTRVSF+ ESPL
Sbjct: 1441 ATTESLRSFDQTVSDTYKSLSSNEETMLE--RTQSIQFEDLLLYTKDLHTRVSFVKESPL 1500

Query: 1501 GQVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFII 1560
            GQVKYTITCYFAKRFEALRKKCCPSELDYV+SLSRCKRWGAQGGKSNVFFAKTLDERFII
Sbjct: 1501 GQVKYTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFII 1560

Query: 1561 KQVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLV 1620
            KQVTKIELESFIQF  +YFKYLSEAIRTGCPTCLAKVVG+YQVSSKHLKGGKESKTDVLV
Sbjct: 1561 KQVTKIELESFIQFGPSYFKYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKESKTDVLV 1620

Query: 1621 MENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLE 1680
            MENLLF HNVTRLYDLKGS RSRYNSDTSGKNKVLLDQNLIESMPT PIF+ SKAKRLLE
Sbjct: 1621 MENLLFRHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTCPIFLGSKAKRLLE 1680

Query: 1681 RAVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGG 1740
            RAVWNDTSFLASIDVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGILGG
Sbjct: 1681 RAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGG 1740

Query: 1741 PKNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENSWD 1776
            PKNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDEN EH++SWD
Sbjct: 1741 PKNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEHDSSWD 1786

BLAST of Sgr019899 vs. NCBI nr
Match: XP_038877586.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Benincasa hispida])

HSP 1 Score: 2934.1 bits (7605), Expect = 0.0e+00
Identity = 1468/1797 (81.69%), Postives = 1586/1797 (88.26%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENK+S+LVGLVKSWMPW+ DP NVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCSKCT+NFIPAPS D + ARE+SEKIRVCNYCFKQWGKEPS +Q+ D A SSAIS
Sbjct: 61   CGRVFCSKCTSNFIPAPSTDPSNAREESEKIRVCNYCFKQWGKEPSDLQDGDKATSSAIS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
            +S SSTS+ STKSGYTCHSAISN ASTPCS+G +Y+DPYC GAITDEQDNL  GK INR 
Sbjct: 121  RSSSSTSMGSTKSGYTCHSAISNSASTPCSNGHQYYDPYCPGAITDEQDNLRTGKTINRT 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTDIDHIAVQGLN 240
            TSLMTSSSS+YGYYRSDDEDDDYYYG+YRSDSESRHISQSDDYY AVNTD      +GLN
Sbjct: 181  TSLMTSSSSFYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNTD------EGLN 240

Query: 241  LDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNNG 300
            L+REN STKGS V FD Q P  +EESSEDSDENDKGSECDFPTY MQNKDNEPMDFQNNG
Sbjct: 241  LERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYVMQNKDNEPMDFQNNG 300

Query: 301  LLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKNV 360
            +LWLPPEPEDEEDEK LLF+DDDD+CAPGEWGYL SS  SGE  M G+ +E+YKKTAKNV
Sbjct: 301  ILWLPPEPEDEEDEKVLLFEDDDDACAPGEWGYLHSSLSSGECHMKGKSSEDYKKTAKNV 360

Query: 361  VEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420
            VEGHFRALV QLLEAENLP+GDE N+DGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV
Sbjct: 361  VEGHFRALVSQLLEAENLPVGDEPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420

Query: 421  KVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFDT 480
            KVKCVAGGKRSESI+VKG+ICKKNVAHRRMTR ISKPRFF+LGGALEYQRV+NHLSSFDT
Sbjct: 421  KVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDT 480

Query: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540
            LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIA C
Sbjct: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIASC 540

Query: 541  TGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCCT 600
            TGANIIP+VDHVASLKLGYCDAFRVETFVEEHD GDGQ  KK TKTLMFFEGCPKPLCCT
Sbjct: 541  TGANIIPTVDHVASLKLGYCDAFRVETFVEEHDNGDGQGGKKSTKTLMFFEGCPKPLCCT 600

Query: 601  ILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNPS 660
            ILLRGAS +ELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+KSPITVALPHNPS
Sbjct: 601  ILLRGASGEELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPITVALPHNPS 660

Query: 661  NIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTLS 720
            NIDRSISAIPGFT P PVK                     +SSII  NVV L+  EPTL 
Sbjct: 661  NIDRSISAIPGFTCPSPVKSPEPEPIMEIGQSGKDAILSRDSSIISHNVVNLNSLEPTLR 720

Query: 721  QLKYNATSTSSSFLKHDVSTA-YHYMLSSKFASEASRSLDIKECFMGMTTIKEEAGVDDD 780
             LK NATS+S S LK DVSTA  +YMLSSKF+SE  R+LD KE  MGM TIKEEAG DDD
Sbjct: 721  LLKSNATSSSMSLLKQDVSTADCNYMLSSKFSSEERRNLDRKEYSMGMMTIKEEAGDDDD 780

Query: 781  TISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKANLV-TKSSREDFPL 840
            T+ S SG GE LS+  NS  IS N LD N  S  D  +  PES+ ANLV TKSSREDFPL
Sbjct: 781  TVRS-SGKGEPLSRFANSIQISRNLLDANAQSSNDSTSQGPESSNANLVSTKSSREDFPL 840

Query: 841  SPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCG 900
            SPSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLG+FLRD+LFDQNYRCHSCG
Sbjct: 841  SPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGQFLRDNLFDQNYRCHSCG 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960
            MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI
Sbjct: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960

Query: 961  MSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020
            MSDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACF+YAAINVQS
Sbjct: 961  MSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYAAINVQS 1020

Query: 1021 VHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNEL 1080
            VHIPPS +EFHHNN +WI KEANEVHNRAK+LFAEVC ALP IPKNESG+EQED GVNEL
Sbjct: 1021 VHIPPSMIEFHHNNLEWINKEANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNEL 1080

Query: 1081 KHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRL 1140
             +YIQELEQI NMEKE+FEVHF EVLSGET VGQ AVDI +INKLHMHVLFLSYIWVQRL
Sbjct: 1081 NNYIQELEQIVNMEKENFEVHFNEVLSGETTVGQSAVDIFQINKLHMHVLFLSYIWVQRL 1140

Query: 1141 SFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKH 1200
            ++  TLR  +S DS SNDIP +E++ LDS+EELVE  LAS +DEFF G DNDSLP++SK 
Sbjct: 1141 NYVGTLRKKHSSDSSSNDIPAIEDKELDSLEELVEFTLASGRDEFFGGFDNDSLPAESKR 1200

Query: 1201 EIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
            E+DL +GGNSG+I++SNE  REKNIDLGGQED+LC SPVN EPLDVGI I +A S+GEYP
Sbjct: 1201 EVDLRRGGNSGKISRSNEANREKNIDLGGQEDDLCFSPVNFEPLDVGIGIQRANSVGEYP 1260

Query: 1261 IMDNLSGTLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAAQL 1320
            IM + SGTLD VWK   HPN+V  E+S S+TN AILEPLAG SD E+C AN  GVE    
Sbjct: 1261 IMTDFSGTLDEVWKGVIHPNNVIFEDSYSRTNFAILEPLAGQSDTERCTAN--GVETTHF 1320

Query: 1321 LHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQGH 1380
            LH+ALVL R D+VET TSS S+ SS YN+KISLLN+PK +I+DYDPAFISS+RELEKQ H
Sbjct: 1321 LHAALVLTRDDSVETITSSTSIPSSAYNTKISLLNIPKSDISDYDPAFISSYRELEKQSH 1380

Query: 1381 LRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSA 1440
            LRLLM VG++DTV+PVYD+EPTSI+AYSL+MPEY AQMSE E+ +EA DT RS P   SA
Sbjct: 1381 LRLLMHVGSSDTVLPVYDEEPTSIVAYSLLMPEYIAQMSEKEKGMEASDTARSLPIIGSA 1440

Query: 1441 NLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLG 1500
             +N + +F +  S+AYK+  SNEE+ LSM R++S QFE LL +TKDLHTRV F+NE+PLG
Sbjct: 1441 KMNLIRTFDEEGSDAYKTLRSNEETILSMPRTESRQFENLLSFTKDLHTRVCFVNETPLG 1500

Query: 1501 QVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560
             VKYT+TCYFAKRFEALRK CCP+ELDY++SLSRCKRWGAQGGKSNVFFAKTLDERFIIK
Sbjct: 1501 PVKYTVTCYFAKRFEALRKMCCPTELDYIRSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560

Query: 1561 QVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVM 1620
            QVTKIELESFIQFA AYF YLSEAIRTGCPTCLAKVVG+YQVSSKHLKGGKE K DVLVM
Sbjct: 1561 QVTKIELESFIQFARAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVM 1620

Query: 1621 ENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLER 1680
            ENLLF HNVTRLYDLKGS R+RYNSDTSGKNKVLLDQNLIESMPTSPIF+ SKAKRLLER
Sbjct: 1621 ENLLFRHNVTRLYDLKGSSRARYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLER 1680

Query: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGP 1740
            AVWNDTSFLAS+DVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGILGGP
Sbjct: 1681 AVWNDTSFLASVDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGP 1740

Query: 1741 KNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENSW 1775
            KNA+PTVISPHQYKKRFRKAMTTYFLMVPDQWPS+TVSPSESHPDLCDEN E ENSW
Sbjct: 1741 KNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSTTVSPSESHPDLCDENSELENSW 1788

BLAST of Sgr019899 vs. NCBI nr
Match: XP_016899810.1 (PREDICTED: LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucumis melo])

HSP 1 Score: 2888.6 bits (7487), Expect = 0.0e+00
Identity = 1459/1798 (81.15%), Postives = 1566/1798 (87.10%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENK+SDLVGLVKSWMPW+ DPANVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCSKCTANFIPA S D T AREDSEKIRVCNYCFKQWGKEPSS+Q+ D A SSA+S
Sbjct: 61   CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
             S SSTS+ STKSGYTCHSAIS+IASTPCS+  +Y+DPYCS AI DE DNL NGK INR 
Sbjct: 121  VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTDIDHIAVQGLN 240
            TSLMTSSSSY GYYRSDDEDDDYYYG+YRSDSESRHISQSDDYY AVN D       GLN
Sbjct: 181  TSLMTSSSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNID------GGLN 240

Query: 241  LDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNNG 300
            L+REN STKGS V FD Q P  +EESSEDSDENDKGSECDFPTY MQNKD+EPMDFQNNG
Sbjct: 241  LERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNG 300

Query: 301  LLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKNV 360
            +LWLPPEPEDEEDEKALLF+DDDD CAPGEWG+L SS  SGE PM G+ +E+YKKTAKNV
Sbjct: 301  ILWLPPEPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNV 360

Query: 361  VEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420
            VEGHFRALV QLLEAENLPIGD  N+DGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV
Sbjct: 361  VEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420

Query: 421  KVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFDT 480
            KVKCVAGGKRSESI+VKG+ICKKNVAHRRMTR ISKPRFF+LGGALEYQRV+NHLSSFDT
Sbjct: 421  KVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDT 480

Query: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540
            LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC
Sbjct: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540

Query: 541  TGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCCT 600
            TGANIIP+VDHVASLKLGYCDAFRVETFVEEHD GDGQ  KK TKTLMFFEGCPKPLCCT
Sbjct: 541  TGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 600

Query: 601  ILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNPS 660
            ILLRGAS DELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+ SPITVALPHNPS
Sbjct: 601  ILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPS 660

Query: 661  NIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTLS 720
            NIDRSISAIPGFT P PVK                     + SII  NV  L+  EPTLS
Sbjct: 661  NIDRSISAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLS 720

Query: 721  QLKYNATSTSSSFLKHDVSTAYHYMLSSKFASEASRSLDIKECFMGMTTIKEEAGVDDDT 780
             L+ NATSTS SFLK D ST    MLSSKF SE  RSLD KE  MGM TIKEEA  D D 
Sbjct: 721  LLESNATSTSFSFLKQDFST----MLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDP 780

Query: 781  ISSCSGIGEALSQCGNS-CYISSNSLDGNTHSFIDLATLRPESTKANLV-TKSSREDFPL 840
            +SS SG  EA S+  NS   +S NSLD NT S  D  +  PE++  +LV TKSSREDFPL
Sbjct: 781  VSS-SGKREASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPL 840

Query: 841  SPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCG 900
            SPSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRD+LFDQNYRCHSCG
Sbjct: 841  SPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCG 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960
            MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI
Sbjct: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960

Query: 961  MSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020
            MSDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS
Sbjct: 961  MSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020

Query: 1021 VHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNEL 1080
            VHIPPS +EFH NN +WI KEANEVHNRAK+LF EVC ALP I KNESG+EQED G +EL
Sbjct: 1021 VHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADEL 1080

Query: 1081 KHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRL 1140
             +YIQELEQI NMEKE+FEVHF +VLSG T V QP VDI EINKLHMH+LFLSYIWVQRL
Sbjct: 1081 NNYIQELEQIINMEKENFEVHFNKVLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRL 1140

Query: 1141 SFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKH 1200
            ++ ATLR  +S D  SNDI TLEE+ L+S+EELVEL LAS +DEFF GCDNDSLP +SK 
Sbjct: 1141 NYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPESKR 1200

Query: 1201 EIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
            E  LG+ GNSG I++ NE  REKNIDLGGQED+ CLSPVNSEP+DVGI I +A S+GEYP
Sbjct: 1201 EAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVGEYP 1260

Query: 1261 IMDNLSGTLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAAQL 1320
            IM +LSGTLDAVWK   HPNS+  E+S S+TNSAILEPLAG SD EKC AN  G+E A L
Sbjct: 1261 IMTDLSGTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTAN--GIETAHL 1320

Query: 1321 LHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQGH 1380
            LHSALVL   D+VET T+S SM  S YN+KISLLN+PK +I+DY   FISS+RELEKQ H
Sbjct: 1321 LHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSH 1380

Query: 1381 LRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSA 1440
            LRLLM VG++DTV+PVYD+EPTSIIAYSL+MPEY AQMSEP + +EA DT RS P   S 
Sbjct: 1381 LRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGSV 1440

Query: 1441 NLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLG 1500
             +NS+ +  +  S+AYK+  SNEE+ LSM R++S+QFE+L+  TKDLHTRV F+NE+PLG
Sbjct: 1441 KMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPLG 1500

Query: 1501 QVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560
             VKYTITCYFAKRFEALRKKCCPSELD+V+SLSRC+RWGAQGGKSNVFFAKTLDERFIIK
Sbjct: 1501 PVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIK 1560

Query: 1561 QVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVM 1620
            QVTKIELESFIQF+ AYF YLS+AI TGCPTCLAKVVG+YQVSSKHLKGGKE K DVLVM
Sbjct: 1561 QVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVM 1620

Query: 1621 ENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLER 1680
            ENLLF HNVTRLYDLKGS RSRYN DTSG NKVLLDQNLIESMPTSPIF+ SKAKRLLER
Sbjct: 1621 ENLLFRHNVTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKAKRLLER 1680

Query: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGP 1740
            AVWNDTSFLASIDVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGILGGP
Sbjct: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGP 1740

Query: 1741 KNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENSWD 1776
            KNA+PTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDEN E ENSWD
Sbjct: 1741 KNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPENSWD 1785

BLAST of Sgr019899 vs. NCBI nr
Match: XP_023515845.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2869.0 bits (7436), Expect = 0.0e+00
Identity = 1442/1798 (80.20%), Postives = 1570/1798 (87.32%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPW+ DP NVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCSKCTANFIP+PS D + AREDSE IRVCNYCFKQWGKEPS++ + D A  SAIS
Sbjct: 61   CGRVFCSKCTANFIPSPSTDPSNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
             S SSTS+ STKSGYTC SAISNI STPCS+  +Y+DP+CSG+I DEQD+L NGK I R 
Sbjct: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTDIDHIAVQGLN 240
            TSLMTSSSSYYGY RSDDED DYYYG+ RSDSESRHI QSD YY + NT+      +GLN
Sbjct: 181  TSLMTSSSSYYGYCRSDDEDGDYYYGMCRSDSESRHIPQSDHYYCSANTE------EGLN 240

Query: 241  LDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNNG 300
            L+REN+STKGS V FD Q P  DEES EDSDENDKGSECDFPTY MQNKDNE MDFQNNG
Sbjct: 241  LERENMSTKGSPVNFDTQSPRGDEESGEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNG 300

Query: 301  LLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKNV 360
            +LWLPPEPEDEEDEKALLF+ DDD+CAPGEWG+LPSS GSGE PM  + + +Y++TAKNV
Sbjct: 301  ILWLPPEPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNV 360

Query: 361  VEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420
            VEGHFRALV QLLEAENLP+GDE N+DGWLDI+TYLSWEAAAVLKPDTSKSG MDPGGYV
Sbjct: 361  VEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYV 420

Query: 421  KVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFDT 480
            KVKCVAGGKR+ESI+VKG+ICKKNVAHRRM ++ISKPRFF+LGGALEYQRV+NHLSSFDT
Sbjct: 421  KVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDT 480

Query: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540
            LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSK ITLVLN+KRSLLERIACC
Sbjct: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACC 540

Query: 541  TGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCCT 600
            TGANI+P+VDHVA LKLGYCD FRV+TFVEEH  GDGQA KK TKTLMFFEGCPKPLCCT
Sbjct: 541  TGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCT 600

Query: 601  ILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNPS 660
            ILLRGAS DELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+KSPI V LPH PS
Sbjct: 601  ILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHTPS 660

Query: 661  NIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTLS 720
            NIDRSISAIPGFT P PVK                     +SS+I  NV  L+  EP+L 
Sbjct: 661  NIDRSISAIPGFTCPSPVKSPEPEPSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLR 720

Query: 721  QLKYNATSTSSSFLKHDVSTA-YHYMLSSKFASEASRSLDIKECFMGMTTIKEEAGVDDD 780
             +K NATSTS SFLK +VSTA  +Y+LSSK ASEA+R+ D +E  MG+ TIKEEA  DDD
Sbjct: 721  LVKSNATSTSLSFLKQNVSTADRNYILSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDD 780

Query: 781  TISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKANLV-TKSSREDFPL 840
            T+SS SG G  LS  GNS +I S+SLD NT S  D    R ES  ANLV TKSSREDFPL
Sbjct: 781  TVSS-SGKGVPLSH-GNSIHIRSSSLDANTQSSNDSTCQRTESLVANLVTTKSSREDFPL 840

Query: 841  SPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCG 900
            SPSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRD+LFDQNYRCHSCG
Sbjct: 841  SPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCG 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960
            MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI
Sbjct: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960

Query: 961  MSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020
            MSDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINV+S
Sbjct: 961  MSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVKS 1020

Query: 1021 VHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNEL 1080
            VHIPPS ++FHHNN +WI KEANEVHNRA+ LFA VC  LP I K+ESG+EQED GVNEL
Sbjct: 1021 VHIPPSIIDFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNEL 1080

Query: 1081 KHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRL 1140
             +YIQELEQI N+EKEDFEVHF +  SGET VGQP VDI EINKLHMHVLFLSYIWVQRL
Sbjct: 1081 NNYIQELEQIINVEKEDFEVHFNKA-SGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRL 1140

Query: 1141 SFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKH 1200
            S+A TL   +S DS SNDIPTLEE+ LDS+EELVEL LA  QD+ F G DNDSLPS+SKH
Sbjct: 1141 SYAVTLGKKHSPDSSSNDIPTLEEKELDSMEELVELTLAFGQDDLFGGFDNDSLPSESKH 1200

Query: 1201 EIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
            ++DL +GGNSGQ+N+ NE EREKNIDLGGQED+LCL  VNSEPLDVG  + +  S GEYP
Sbjct: 1201 DVDLDRGGNSGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYP 1260

Query: 1261 IMDNLSGTLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAAQL 1320
             M +LSGTLDAVWK   HPNS+  ++   QTNS+ILEPLAG  D EKC AN  GVE A+L
Sbjct: 1261 SMTDLSGTLDAVWKGALHPNSIQFDDRYPQTNSSILEPLAG-PDMEKCTAN--GVETARL 1320

Query: 1321 LHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQGH 1380
            L+SALVL RVD+VETCTS MSM SS Y+SKISLLN+PKP+I+DYDPA I+SFRELEKQGH
Sbjct: 1321 LYSALVLTRVDSVETCTSLMSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGH 1380

Query: 1381 LRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSA 1440
            LR  M V ++DTV+PVYD+EPTSIIAYSL+MPEYHAQMSEPERV+EA DT RS P   S 
Sbjct: 1381 LRFFMHVDSSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSV 1440

Query: 1441 NLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLG 1500
             ++S+ +  +  S+ Y++  SNEE+ LSMAR++S+QFE+LLL+TKDLHTRV F+NE+PLG
Sbjct: 1441 KMSSIKTCDEEGSDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLG 1500

Query: 1501 QVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560
             VKYTITCYFAKRFEALRKKCCPSELDYV+SLSRCKRWGAQGGKSNVFFAKTLDERFIIK
Sbjct: 1501 PVKYTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560

Query: 1561 QVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVM 1620
            QVTKIELESFIQFA AYF YLSE+IRTGCPTCLAKVVG+YQVS+KHLKGGKE K DVLVM
Sbjct: 1561 QVTKIELESFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVM 1620

Query: 1621 ENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLER 1680
            ENLLFGHNVTRLYDLKGS RSRYNSDTSGKNKVLLDQNLIESMPTSPIF+  KAKRLLER
Sbjct: 1621 ENLLFGHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLER 1680

Query: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGP 1740
            AVWNDTSFLASIDVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGILGGP
Sbjct: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGP 1740

Query: 1741 KNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENSWD 1776
            KNA+PTVISPHQYKKRFRKAMTTYFLMVPDQWPSST+SPSESHPDLCDEN E ENSWD
Sbjct: 1741 KNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTISPSESHPDLCDENSEPENSWD 1786

BLAST of Sgr019899 vs. NCBI nr
Match: XP_022961087.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucurbita moschata])

HSP 1 Score: 2867.0 bits (7431), Expect = 0.0e+00
Identity = 1442/1798 (80.20%), Postives = 1570/1798 (87.32%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPW+ DP NVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCSKCTANFIP+PS D T AREDSE IRVCNYCFKQWGKEPS++ + D A  SAIS
Sbjct: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
             S SSTS+ STKSGYTC SAISNI STPCS+  +Y+DP+CSG+I DEQD+L NGK I R 
Sbjct: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTDIDHIAVQGLN 240
            TSLMTSSSSYYGY RSDDED DYYYG+ RSDSESRHI QSD YY + NT+      +GLN
Sbjct: 181  TSLMTSSSSYYGYCRSDDEDGDYYYGMCRSDSESRHIPQSDHYYCSANTE------EGLN 240

Query: 241  LDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNNG 300
            L+REN+STKGS V FD Q P  DEESSEDSDENDKGSECDFPTY MQNKDNE MDFQNNG
Sbjct: 241  LERENMSTKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNG 300

Query: 301  LLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKNV 360
            +LWLPPEPEDEEDEKALLF+ DDD+CAPGEWG+LPSS GSGE PM  + + +Y++TAKNV
Sbjct: 301  ILWLPPEPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNV 360

Query: 361  VEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420
            VEGHFRALV QLLEAENLP+GDE N+DGWLDI+TYLSWEAAAVLKPDTSKSG MDPGGYV
Sbjct: 361  VEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYV 420

Query: 421  KVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFDT 480
            KVKCVAGGKR+ESI+VKG+ICKKNVAHRRM ++ISKPRFF+LGGALEYQRV+NHLSSFDT
Sbjct: 421  KVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDT 480

Query: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540
            LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSK ITLVLN+KRSLLERIACC
Sbjct: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACC 540

Query: 541  TGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCCT 600
            TGANI+P+VDHVA LKLGYCD FRV+TFVEEH  GDGQA KK TKTLMFFEGCPKPLCCT
Sbjct: 541  TGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCT 600

Query: 601  ILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNPS 660
            ILLRGAS DELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+KSPI V LPH+PS
Sbjct: 601  ILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPS 660

Query: 661  NIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTLS 720
            NIDRSIS IPGFT P PVK                     +SS+I  NV  L+  EP+L 
Sbjct: 661  NIDRSISTIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLR 720

Query: 721  QLKYNATSTSSSFLKHDVSTA-YHYMLSSKFASEASRSLDIKECFMGMTTIKEEAGVDDD 780
             +K NATSTS SFLK +VSTA  +YMLSSK ASEA+R+ D +E  MG+ TIKEEA  DDD
Sbjct: 721  LVKSNATSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDD 780

Query: 781  TISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKANLV-TKSSREDFPL 840
            T+SS SG G  LS  GNS +I S+SLD NT S  D    RPES  ANLV TKSSREDFPL
Sbjct: 781  TVSS-SGKGVPLSH-GNSIHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPL 840

Query: 841  SPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCG 900
            SPSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRD+LFDQNYRCHSCG
Sbjct: 841  SPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCG 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960
            MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI
Sbjct: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960

Query: 961  MSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020
            MSDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINV+S
Sbjct: 961  MSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVKS 1020

Query: 1021 VHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNEL 1080
            VHIPPS ++FHHNN +WI KEANEVHNRA+ LFA VC  LP I K+ESG+EQED GVNEL
Sbjct: 1021 VHIPPSIIDFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNEL 1080

Query: 1081 KHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRL 1140
             +YIQELEQI N+EKEDFEVHF +  SGET VGQP VDI EINKLHMHVLFLSYIWVQRL
Sbjct: 1081 NNYIQELEQIINVEKEDFEVHFNKA-SGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRL 1140

Query: 1141 SFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKH 1200
            S+A TL   +S DS SNDIPTLEE+ LDS+EELVEL LA   D+ F G DNDSLPS+SKH
Sbjct: 1141 SYAVTLGKKHSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKH 1200

Query: 1201 EIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
            ++DL +GGNSGQ+N+ NE EREKNIDLGGQED+LCL  VNSEPLDVG  + +  S GEYP
Sbjct: 1201 DVDLDRGGNSGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYP 1260

Query: 1261 IMDNLSGTLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAAQL 1320
             M +LSGTLDAVWK   HPNS+  ++   QT+S+ILEPLAG  D EKC AN  GVE A+L
Sbjct: 1261 SMTDLSGTLDAVWKGALHPNSMLFDDRYPQTSSSILEPLAG-PDMEKCTAN--GVETARL 1320

Query: 1321 LHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQGH 1380
            L+SALVL  VD+VETCTS  SM SS Y+SKISLLN+PKP+I+DYDPA I+SFRELEKQGH
Sbjct: 1321 LYSALVLTGVDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGH 1380

Query: 1381 LRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSA 1440
            LRL M V ++DTV+PVYD+EPTSIIAYSL+MPEYHAQMSEPERV+EA DT RS P   S 
Sbjct: 1381 LRLFMHVDSSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSV 1440

Query: 1441 NLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLG 1500
             ++S+ +  +  S+ Y++  SNEE+ LSMAR++S+QFE+LLL+TKDLHTRV F+NE+PLG
Sbjct: 1441 KMSSIKTCDEEGSDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLG 1500

Query: 1501 QVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560
             VKYTITCYFAKRFEALRKKCCPSELDYV+SLSRCKRWGAQGGKSNVFFAKTLDERFIIK
Sbjct: 1501 PVKYTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560

Query: 1561 QVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVM 1620
            QVTKIELESFIQFA AYF YLSE+IRTGCPTCLAKVVG+YQVS+KHLKGGKE K DVLVM
Sbjct: 1561 QVTKIELESFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVM 1620

Query: 1621 ENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLER 1680
            ENLLFGHNVTRLYDLKGS RSRYNSDTSGKNKVLLDQNLIESMPTSPIF+  KAKRLLER
Sbjct: 1621 ENLLFGHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLER 1680

Query: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGP 1740
            AVWNDTSFLASIDVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGILGGP
Sbjct: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGP 1740

Query: 1741 KNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENSWD 1776
            KNA+PTVISPHQYKKRFRKAMTTYFLMVPDQWPSST+SPSESHPDLCDEN E ENSWD
Sbjct: 1741 KNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTISPSESHPDLCDENSEPENSWD 1786

BLAST of Sgr019899 vs. ExPASy Swiss-Prot
Match: Q9LUM0 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)

HSP 1 Score: 1697.2 bits (4394), Expect = 0.0e+00
Identity = 965/1818 (53.08%), Postives = 1212/1818 (66.67%), Query Frame = 0

Query: 9    SDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRRCGRVFCSK 68
            S++VGL+KSW+PW+ +PA VSRDFWMPD+SCRVCY+CD QFT INRRHHCR CGRVFC K
Sbjct: 12   SEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGK 71

Query: 69   CTANFIPAPSIDSTTAREDSEKIRVCNYCFKQW--GKEPSSVQNDDNAVSSAISQSPSST 128
            CTAN IP    D  T RED E+IRVCNYCF+QW  G     V N      + +S SPS T
Sbjct: 72   CTANSIPFAPSDLRTPREDWERIRVCNYCFRQWEQGDGGPHVSN-----ITELSTSPSET 131

Query: 129  SLSSTKSGYTCHSAISNIASTPCSSG--PRYH---DPYCSGAITDEQDNLLNGKCINRGT 188
            SL S+K+  T +S+   + S P   G   R H   D    G  + E      GK  +R +
Sbjct: 132  SLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRRS 191

Query: 189  SLMTS-----SSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTD---IDH 248
            S + +     S       RSDDE D+  YG Y++D E+ H  ++++YYG +  +   ID 
Sbjct: 192  SFIATDVEDPSRFALNSIRSDDEYDE--YGAYQTDIETSHSPRANEYYGPMEYNGMGIDD 251

Query: 249  IAVQGLNLDRENISTKGSSVIFDNQLPGADEESSED---SDENDKGSECD--FPTYCMQN 308
            +  + L  +  +  +   S +    L     E SE      E+D   EC+   P     +
Sbjct: 252  VPCKHLGGETADQKSLSGSPLIHQCLESLIREGSEQFQKKSEHDGRDECEASSPADISDD 311

Query: 309  KDNEPMDFQNNGLLWLPPEPEDEEDEK--ALLFDDDDDSCAPGEWGYL--PSSFGSGEYP 368
            +  EP+DF+NNGLLW+PPEPE+EEDE+  AL  ++D++  A GEWGYL   +SFGSGEY 
Sbjct: 312  QVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSGEYR 371

Query: 369  MNGRPNEEYKKTAKNVVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVL 428
               R  EE+KK  KNVV+GHFRAL+ QLL+ EN+ + DE  K+ WL+IIT LSWEAA +L
Sbjct: 372  GEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANLL 431

Query: 429  KPDTSKSGGMDPGGYVKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGG 488
            KPD SKSGGMDPGGYVKVKC+A G R +S+VVKGV+CKKNV +RRM+ KI K R  ILGG
Sbjct: 432  KPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILGG 491

Query: 489  ALEYQRVSNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNIT 548
             LEYQRVSN LSSFDTLLQQE DHLKMAVAKI A  PN+LLVEKSVSR AQEYLL+K+I+
Sbjct: 492  GLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDIS 551

Query: 549  LVLNIKRSLLERIACCTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLT 608
            LVLNIKR LL+RIA CTGA IIPSVDH++S KLGYC+ FRV+ + EEH G  GQ  KK+ 
Sbjct: 552  LVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEH-GSTGQVGKKVV 611

Query: 609  KTLMFFEGCPKPLCCTILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPE 668
            KTLM+FE CPKPL  TILLRGA+EDELK+VKHVVQY  FAAYHLALET FLADEGAS PE
Sbjct: 612  KTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS-PE 671

Query: 669  LPMKSPITVALPHNPSNIDRSISAIPGFTGPPPVKESSIIGDNVVKLDYSEPTLSQLKYN 728
            LP+ SPITVALP   ++I+RSIS +PGFT     K  +++     +   S P +S+L   
Sbjct: 672  LPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVP-VSELLST 731

Query: 729  ATSTS-----------SSFLKHDVSTAY---HYMLSSKFASEA--SRSLDIKECFMGMTT 788
             T+ S           S +   +++ ++    + +S         SR+ D+    + + T
Sbjct: 732  TTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSGRSVPVDT 791

Query: 789  IKEEAG----VDDDTISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKA 848
              +++      D+ T +S    G+   +   S  I ++ +  N  +  +L   + ++ + 
Sbjct: 792  PADKSNPIVVADETTNNSLHLSGQGFVR--KSSQIGTSIMVENQDNGSELTIAQQQNNEK 851

Query: 849  NLVTKSSREDFPLSPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRD 908
               T+S +E+FP SPSDHQ+ILVSLS+R VWKGT+C+R+HL RIKYYGSFDKPLGRFLRD
Sbjct: 852  PKETQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLRD 911

Query: 909  HLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCP 968
            HLFDQ+YRC SC MPSEAHVHCYTH QGSLTIS KKL D+ LPGE EGKIWMWHRCL+CP
Sbjct: 912  HLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRCLRCP 971

Query: 969  RTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRT 1028
            R NGFPPAT RV+MSDAAWGLSFGKFLELSFSNHAAASRVA CGH LHRDCLRFYGFG  
Sbjct: 972  RLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGNM 1031

Query: 1029 VACFHYAAINVQSVHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNE 1088
            VACF YA I+V SV++PPS L F++ N+DWI +E +EV  RA+ LF+EV NA+ QI   E
Sbjct: 1032 VACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQIA--E 1091

Query: 1089 SGSEQEDLGVNELKHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHM 1148
             G           +  I ELE++   EK +FE + +++L  E   GQP VDILE+ ++H 
Sbjct: 1092 KG----------FRRRIGELEEVLQKEKAEFEENMQKILHREVNEGQPLVDILELYRIHR 1151

Query: 1149 HVLFLSYIWVQRLSFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFS 1208
             +LF SY+W  RL  A+TL    + D    +    E+  L   + L E+N  +  +   +
Sbjct: 1152 QLLFQSYMWDHRLINASTLHKLENSDDTKRE--ENEKPPLAKSQTLPEMNAGT--NSLLT 1211

Query: 1209 GCDNDSLPSDSKHEIDLGKGGNSGQINQSNEMEREKNIDL------GGQEDELCLSPVNS 1268
            G + +  P       D    G++G +N + + E + N DL      GG+       P  S
Sbjct: 1212 GSEVNLNP-------DGDSTGDTGSLN-NVQKEADTNSDLYQEKDDGGEVSPSKTLPDTS 1271

Query: 1269 EPLDVGITIGKAMSMGEYPIMDNLSGTLDAVW------KAETHPNSVTSEESGSQTNSAI 1328
             PL+  + + +  S G+  +M NLS TLDA W        E   N+  S    + +NS+ 
Sbjct: 1272 YPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVEIPTNNKVSLPPSTMSNSST 1331

Query: 1329 LEPLAGHSDFEKCIANLVGVEAAQLLHSALVLRRVDTVETCTSSMSMS-SSVYNS--KIS 1388
              P++               + A  +  AL  +  +  E   S +S+   + Y S  K  
Sbjct: 1332 FPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSEDSVSWLSVPFLNFYRSINKNF 1391

Query: 1389 LLNVPKPE-IADYDPAFISSFRELEKQGHLRLLMRVGANDTVIPVYDDEPTSIIAYSLVM 1448
            LL+  K +   ++ P +ISSFRE E QG  RLL+ VG ND V+PVYDDEPTS+IAY+L+ 
Sbjct: 1392 LLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDIVVPVYDDEPTSMIAYALMS 1451

Query: 1449 PEYHAQMSEPERVIEAGDTTRSSPPHDSANLNSLNSFKDAVSNAYKSHSSNEESTLSMAR 1508
            PEY  Q S        G++  S P    + LN      D + +  +S+ S +ES LS++ 
Sbjct: 1452 PEYQRQTS------AEGESLVSYP----SELNIPRPVDDTIFDPSRSNGSVDESILSISS 1511

Query: 1509 SQSIQFEELLLYTKDLHTRVSFLNESPLGQVKYTITCYFAKRFEALRKKCCPSELDYVKS 1568
            S+S    + L YTK LH RVS+  +  LG+VKYT+TCY+AKRFEALR  C PSEL+Y++S
Sbjct: 1512 SRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICLPSELEYIRS 1571

Query: 1569 LSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFATAYFKYLSEAIRTGCPT 1628
            LSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+FA AYFKYLSE+I T  PT
Sbjct: 1572 LSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLSESISTKSPT 1631

Query: 1629 CLAKVVGIYQVSSKHLKGGKESKTDVLVMENLLFGHNVTRLYDLKGSCRSRYNSDTSGKN 1688
            CLAK++GIYQV++K LK GKE+K DVL+MENLLFG  V RLYDLKGS R+RYN D+SG N
Sbjct: 1632 CLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRARYNPDSSGSN 1691

Query: 1689 KVLLDQNLIESMPTSPIFVASKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELV 1748
            KVLLDQNLIE+MPTSPIFV +KAKRLLERAVWNDT+FLA  DVMDYSLLVG+DEEK ELV
Sbjct: 1692 KVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVGVDEEKNELV 1751

Query: 1749 LGIIDFMRQYTWDKHLETWVKNSGILGGPKNASPTVISPHQYKKRFRKAMTTYFLMVPDQ 1767
            LGIIDF+RQYTWDKHLE+WVK +GILGGPKN +PTVISP QYK+RFRKAMTTYFLMVPDQ
Sbjct: 1752 LGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAPTVISPKQYKRRFRKAMTTYFLMVPDQ 1782

BLAST of Sgr019899 vs. ExPASy Swiss-Prot
Match: Q0WUR5 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)

HSP 1 Score: 1650.6 bits (4273), Expect = 0.0e+00
Identity = 955/1832 (52.13%), Postives = 1203/1832 (65.67%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            M   ++K    V +VKSW+P K + +N+SRDFWMPD+SC VCY+CD+QFT  NRRHHCR 
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFC+KC AN IP+PS ++  + E+ E+IRVCNYC+KQW +      N  + +S   S
Sbjct: 61   CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAIT-------DEQDNLLN 180
             SPS+ S++ST    T +S+   I ST   S     +P  S  ++        EQ N  +
Sbjct: 121  SSPSARSVAST----TSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQNAKS 180

Query: 181  GKCINRGTSLMTSSSSYYGYY-----RSD---DEDDDYYYGIYRSDSESRHISQSDDYYG 240
             +  +    ++ SS +   ++     RSD   D+DDD     Y+SD  ++  +Q +DYYG
Sbjct: 181  RRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDD-----YQSDF-AQSYAQGNDYYG 240

Query: 241  AVNTD-IDHIAVQGLNLD-RENISTKGSSVIFDNQLPGADEESSEDSDENDKG----SEC 300
            A+N D +DHI       D    I    S    D  L   + E+ + + + + G     E 
Sbjct: 241  AINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKEG 300

Query: 301  DFPTYCMQNKDNEPMDFQNNGLLWLPPEPEDEEDEK-ALLFDDDDDSCAPGEWGYLPSSF 360
              P  C ++ + E +DF+++GLLWLPPEPE+EEDE+ A+L DDD D    G+WGYL  S 
Sbjct: 301  SPP--CEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSN 360

Query: 361  GSGEYPMNGRPNEEYKKTAKNVVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSW 420
               E   + +  ++     KNVVEGHFRALV QLLE +NLP+ +E +++GWLDIIT LSW
Sbjct: 361  SFNEKDFHSK--DKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSW 420

Query: 421  EAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPR 480
            EAA +LKPDTSKSGGMDPGGYVKVKC+  G+RSES+VVKGV+CKKNVAHRRMT KI KPR
Sbjct: 421  EAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPR 480

Query: 481  FFILGGALEYQRVSNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYL 540
              ILGGALEYQR+SN LSSFDTLLQQE+DHLKMAVAKID+H+P++LLVEKSVSR AQEYL
Sbjct: 481  LLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYL 540

Query: 541  LSKNITLVLNIKRSLLERIACCTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQ 600
            L+K+I+LVLNIKRSLLERI+ CTGA I+PS+D + S KLGYCD F VE FVE H     Q
Sbjct: 541  LAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETH-VSPCQ 600

Query: 601  ASKKLTKTLMFFEGCPKPLCCTILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADE 660
             +KK+ KTLMFF+GCPKPL CTILL+GA EDELK+VKHV+QY  FAAYHLALET FLADE
Sbjct: 601  VAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADE 660

Query: 661  GASVPELPMKSPITVALPHNPSNIDRSISAIPGFT-------------GPPPVKESSIIG 720
            GAS+ ELP+++PITVALP  PS ++RSIS IPGFT             G P      + G
Sbjct: 661  GASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTG 720

Query: 721  DNVVKLDYSEPTLSQLKYNATSTSSSFLKHDVSTAY---HYMLSSK----FASEASRSLD 780
            +      + +    +L  N     S  L H++ T Y      ++SK      +  SR L 
Sbjct: 721  NFTSSKTHFQ---GKLDGNDRIDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLS 780

Query: 781  IKECFMGMTTIKEEAGVDDDTISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLR 840
                        EE  V  D  S  SG  E ++  G   Y + +++ GN           
Sbjct: 781  FH---------VEEPSVQKDQWSVLSGATEQVTDGG---YTNDSAVIGN----------- 840

Query: 841  PESTKANLVTKSSREDFPLSPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPL 900
             ++       +SS+ DF  S SDHQ+ILVSLSTRCVWKG++C+RAHLLRIKYYGSFDKPL
Sbjct: 841  -QNFNRQEQMESSKGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPL 900

Query: 901  GRFLRDHLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWH 960
            GRFLRD+LFDQ+  C SC MP+EAH+HCYTH QGSLTIS KKLP+  LPG+ EGKIWMWH
Sbjct: 901  GRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPE-LLPGQREGKIWMWH 960

Query: 961  RCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRF 1020
            RCLKCPR NGFPPATRR++MSDAAWGLSFGKFLELSFSNHAAASRVA CGH LHRDCLRF
Sbjct: 961  RCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRF 1020

Query: 1021 YGFGRTVACFHYAAINVQSVHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALP 1080
            YGFGR VACF YA+IN+ +V +PP+KL F++ N++W+ KE+ EV  +A+ LF EV  AL 
Sbjct: 1021 YGFGRMVACFRYASINIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALS 1080

Query: 1081 QIPKNESGSEQEDLGVNELKHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILE 1140
            QI     G+  +    N++K  ++EL  +    K++++   +++L+     GQP +DIL 
Sbjct: 1081 QISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQMLN-VVKDGQPTIDILL 1140

Query: 1141 INKLHMHVLFLSYIWVQRLSFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASR 1200
            INKL   ++F SY W + L+ AA +  +N L++  N  P +  R + S+E+L +  + S 
Sbjct: 1141 INKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSI 1200

Query: 1201 QDEFFSGCDNDSLPSDSKHEIDLGKG----GNSGQINQSNEMEREKNIDL--------GG 1260
                     NDSL  D+ +E  L +G      SG+     ++  ++  D         GG
Sbjct: 1201 PTHV--AICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDPSEGG 1260

Query: 1261 QEDELCLSPVNSEPLDVGITIGKAMSMGEYPIMDNLSGTLDAVWKAE--THPNSVTSEES 1320
            +++ +  S V          +  A +  ++   D LS TLDA W  E  T  N +    S
Sbjct: 1261 KDNFVESSQV----------VKPAHTESQFQATD-LSDTLDAAWIGEQTTSENGIFRPPS 1320

Query: 1321 -GSQTNSAILEPLAGHSDFEKCIANLVGVEAAQLLHSALVLRRVDTVETCTSSMSM-SSS 1380
              + TN   +  L            L+G E      S L  +   T +  T+ + + S S
Sbjct: 1321 RAASTNGTQIPDL-----------RLLGSE------SELNFKGGPTNDEHTTQVQLPSPS 1380

Query: 1381 VYNS--KISLLNVPKPEIADYDPAFISSFRELEKQGHLRLLMRVGANDTVIPVYDDEPTS 1440
             Y S  K   LN  K  +A+  P ++SS+RELE +   RLL+ +G ND V+PVYDDEPTS
Sbjct: 1381 FYYSLNKNYSLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTS 1440

Query: 1441 IIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSANLNSLNSFKDAVSNAYKSHSSNE 1500
            IIAY+L   EY AQMS  ++  +  D+  S    DS NL SLNS  D   +  +S SS +
Sbjct: 1441 IIAYALTSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSAD 1500

Query: 1501 ESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLGQVKYTITCYFAKRFEALRKKCCP 1560
            E    +  S         LY KDLH R+SF +E P G+VKY++TCY+AK FEALR  CCP
Sbjct: 1501 EQVSQLLHSS--------LYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCP 1560

Query: 1561 SELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFATAYFKYLSE 1620
            SE D+++SL RC++WGAQGGKSNVFFAK+LD+RFIIKQVTK ELESFI+F  AYFKYL+E
Sbjct: 1561 SETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTE 1620

Query: 1621 AIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVMENLLFGHNVTRLYDLKGSCRSRY 1680
            +I T  PT LAK++GIYQVSSKHLKGGKE K DVLVMENLLF  N TRLYDLKGS R+RY
Sbjct: 1621 SISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARY 1680

Query: 1681 NSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLERAVWNDTSFLASIDVMDYSLLVGL 1740
            N DTSG N VLLDQNL+E+MPTSPIFV SKAKRLLERAVWNDTSFLASI VMDYSLLVG+
Sbjct: 1681 NPDTSGSNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGV 1740

Query: 1741 DEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGPKNASPTVISPHQYKKRFRKAMTT 1773
            DEE+ ELVLGIIDFMRQYTWDKHLETWVK SG+LGGPKN++PTVISP QYKKRFRKAMT 
Sbjct: 1741 DEERNELVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTA 1749

BLAST of Sgr019899 vs. ExPASy Swiss-Prot
Match: Q9SSJ8 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 695/1778 (39.09%), Postives = 990/1778 (55.68%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDP-----ANVSRDFWMPDKSCRVCYDCDSQFTFINRR 60
            MG P+  L DL+  V+SW+           ++  +DF +     ++C+DC    T + + 
Sbjct: 1    MGIPDGSLLDLIDKVRSWITSDSSDSLFLLSSSKQDFGIMPIVSKMCHDCG---TKVEQG 60

Query: 61   HHCRRCGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAV 120
            + C  CG  +C  C           S T     +  R C+   ++   +     +  ++ 
Sbjct: 61   YCCLSCGSCWCKSC-----------SDTEESKMKLCRECDAEVRELRVKSYDKVHPRDSP 120

Query: 121  SSAISQSPSSTSLSSTKSGYTCHSAISNIASTPC--SSGPRYHDPYCSGAITDEQDNLLN 180
                S +  S SL+S+     C     N+AS  C  S G      YC             
Sbjct: 121  DPPSSLATESESLASSLEIRDCR----NMASIRCYPSRGEEEEARYC------------- 180

Query: 181  GKCINRGTSLMTSSSSYYGYYRSDDEDDDY-----YYGIYRSDSESRHISQSDDYYGAVN 240
                  G  L++ SS  Y    SD E          +    S   S H S   + +  + 
Sbjct: 181  ------GKQLLSPSSDNY-QDSSDIESGSVSARHELFSCKSSAGSSPHDSPLRNNFSPLG 240

Query: 241  TDIDHIAVQGLNLDRENISTKGSSVIFDNQL-PGADEESSEDSDENDKGSECDFPTYCMQ 300
              + H A    +    +       ++ DN + PG      ED +E              +
Sbjct: 241  RFVQH-AKDLRSPTVCSFDNHQEQLLADNLVKPGQGVLEQEDHEEE-------------E 300

Query: 301  NKDNEPMDFQNNGLLWLPPEPEDEEDE---KALLFDDDDDSCAPGEWGYLPSSFGSGEYP 360
            +K  +P+DF+NNG +W PP PEDE D+       +DD+DD        +  SS  S   P
Sbjct: 301  DKLQQPLDFENNGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHIP 360

Query: 361  MNGRPNEEYKKTAKNVVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVL 420
               +  E   +  + VV  HFRALV +LL  E L   D+ +   WLDI+T L+W+AA  +
Sbjct: 361  TKEKLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFV 420

Query: 421  KPDTSKSGGMDPGGYVKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGG 480
            KPDT   G MDPG YVK+KCVA G ++ESI+++G++C KN+ H+RM  +   PR  +L G
Sbjct: 421  KPDTRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAG 480

Query: 481  ALEYQRVSNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNIT 540
            +LEYQRV+  L+SF+TLLQQE +H+K  +AKI++  PNVLLVEKS S +AQ+YLL K I+
Sbjct: 481  SLEYQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEIS 540

Query: 541  LVLNIKRSLLERIACCTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLT 600
            LVLN+KRSLL+RIA CTGA + PS+D +++ +LG+C+ FR E  +E+H+ G+ Q+++K +
Sbjct: 541  LVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGN-QSNRKPS 600

Query: 601  KTLMFFEGCPKPLCCTILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPE 660
            +TLM+FEGCP+ L CT++LRG+  +ELK+VKHV+QYA FAAYHL+LET FLADEGAS+P+
Sbjct: 601  RTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPK 660

Query: 661  LPMKSPITVALPHNPSNIDRSISAIPGFTGPPPVKESSIIGDNVVKLDYSEPTLSQLKYN 720
            + +K P  V        ID  IS I   T  P   +S  + +     D  E T    ++ 
Sbjct: 661  IRLKQPGMVRTASQRRIIDEGISLI---TQSPTETDSQALLETAAHED--EHTAPMPEHE 720

Query: 721  ATSTSSSFLKHDVSTAYHYMLSSKFASEASRSLD---IKECFMGMTTIKEEAGVDDDTIS 780
               +    L  D      +  SS+  +E S +L+            +  +   + + T+ 
Sbjct: 721  VCES----LCEDFDPTQIFPPSSEVETEQSDTLNGDFANNLVTRSYSSNQLNDLHEPTLC 780

Query: 781  SCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKANLVTKSSREDFPLSPSD 840
              S I E  +Q       S    +G       L   +      +        ++  +   
Sbjct: 781  LSSEIPETPTQ-----QPSGEEDNGRGEEENQLVNPQDLPQHESFYEDDVSSEYFSAADS 840

Query: 841  HQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCGMPSE 900
            HQ+ILVS S+RCV K ++C+R+ LLRIK+YGSFDKPLGR+L+D LFD+   C SC    +
Sbjct: 841  HQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELVD 900

Query: 901  AHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDA 960
            AHV CY+H  G+LTI+ ++LP   LPGE +GKIWMWHRCL+C   +G PPATRRV+MSDA
Sbjct: 901  AHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSDA 960

Query: 961  AWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIP 1020
            AWGLSFGKFLELSFSNHA A+RVA CGH L RDCLRFYGFG  VA F Y+ IN+ +V +P
Sbjct: 961  AWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLLP 1020

Query: 1021 PSKLEFH-HNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNELKHY 1080
            PS LEF+ H  ++WI  EA E+  + ++++ E+ + L ++ +  S  E E     +L   
Sbjct: 1021 PSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQSEACDLHSR 1080

Query: 1081 IQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRLSFA 1140
            I  L      EK++++   + +   E +  Q ++DILE+N+L   ++  ++ W       
Sbjct: 1081 IIGLIDQLVKEKDEYDDALQPIFE-ENLQIQGSLDILELNRLRRALMIGAHAW------- 1140

Query: 1141 ATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKHE-- 1200
                +H                      +L  LN   ++   F   D+++  +   H+  
Sbjct: 1141 ----DH----------------------QLYLLNSQLKKASVFKTGDDNAPRNPEMHDPP 1200

Query: 1201 -IDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
             ID      S + ++ +  + E N D    E+        SE +D    +G   ++ +  
Sbjct: 1201 KIDRRMQEGSDERDEQSHTDSEANGDNKDPENIPSPGTSLSERID-SAWLGSFQNLEKAE 1260

Query: 1261 IMDNLSG--TLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAA 1320
             +    G   +++  +    P  V S +S  +    I + L   S +         +   
Sbjct: 1261 TIAETEGFSAVNSSLRRLARPIRVQSFDSAIRFQERIQKGLPPSSLY---------LSTL 1320

Query: 1321 QLLHSALVLRRV--DTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELE 1380
            +  H++   R +  D V     + S    +   K+ L       I    P +ISS  ++ 
Sbjct: 1321 RSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQKLDL-------IVGSAPTYISSASQM- 1380

Query: 1381 KQGHLRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPP 1440
              G   L+ + G ND V+PVYDD+P S+++Y++   EY       E ++  G  + SS  
Sbjct: 1381 ADGARMLIPQRGLNDIVVPVYDDDPASVVSYAINSKEY------KEWIVNKGLASSSS-- 1440

Query: 1441 HDSANLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNE 1500
              S+NLN+  S   A S  ++S S + +         S    +    T     R S  + 
Sbjct: 1441 --SSNLNNRESEPSAFS-TWRSLSMDVDYIQHAVYGSSQDDRKSPHLTISFSDRASSSST 1500

Query: 1501 SPLGQVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDER 1560
            +  G+VK+++TCYFA +F+ LRK CCPSE+D+V+SLSRC+RW AQGGKSNV+FAK+LDER
Sbjct: 1501 ATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFAKSLDER 1560

Query: 1561 FIIKQVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTD 1620
            FIIKQV K EL+SF  FA  YFKYL E++ +G PTCLAK++GIYQVS KH KGGKE+K D
Sbjct: 1561 FIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGGKETKMD 1620

Query: 1621 VLVMENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKR 1680
            ++VMENL +   ++R+YDLKGS RSRYN +TSG +KVLLD NL+E++ T PIF+ SKAKR
Sbjct: 1621 LMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLETLRTEPIFLGSKAKR 1648

Query: 1681 LLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGI 1740
             LERA+WNDT+FLAS+DVMDYSLLVG DEE++ELVLGIIDFMRQYTWDKHLETWVK SGI
Sbjct: 1681 SLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGIIDFMRQYTWDKHLETWVKASGI 1648

Query: 1741 LGGPKNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPS 1752
            LGGPKNASPT++SP QYK+RFRKAMTTYFL VP+ W S
Sbjct: 1741 LGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEPWTS 1648

BLAST of Sgr019899 vs. ExPASy Swiss-Prot
Match: Q9XID0 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.5e-200
Identity = 529/1512 (34.99%), Postives = 771/1512 (50.99%), Query Frame = 0

Query: 263  DEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNNGLLWLPPEPEDEEDEKALLFDDD 322
            DE+S E+S EN       F +    + D+ P        +W PPEPE+ EDE   +F DD
Sbjct: 69   DEKSKENSVEN-----VQFLSDREDDSDDVP--------VWEPPEPENPEDEVDGVFADD 128

Query: 323  DDSCAPGEWGYLPSSFG--SGEYPMNGRPNEEYKKTAKNVVEGHFRALVGQLLEAENLPI 382
            DD C  G      S  G  S E     +  EE ++      +  F+ +V QL+++    I
Sbjct: 129  DDDCCDGSKWNKASLLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIKSAGFSI 188

Query: 383  GDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIVVKGVI 442
             +ES    W +I+  L WEAA++LKP       +DP  Y+KVKC+A G   +S V KG++
Sbjct: 189  -EESGY--WFEIVARLCWEAASMLKP-AIDGKSVDPTEYIKVKCIATGSCVDSEVFKGLV 248

Query: 443  CKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFDTLLQQE---LDHLKMAVAKID 502
             KK+ A + M  K   PR  ++ G L +    +  SS  ++ Q     L ++K  V  I+
Sbjct: 249  FKKHAALKHMATKYEHPRIMLVEGVLGHP--ISGFSSLQSVNQDNEYLLKYVKPVVDIIE 308

Query: 503  AHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANIIPSVDHVASLKL 562
            A  P+V+LVEKSVSR  Q+ +L K +TLV ++K   L+RI+ C G+ I+ SVD ++S KL
Sbjct: 309  ASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPIL-SVDSLSSQKL 368

Query: 563  GYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCCTILLRGASEDELKQVKHV 622
             +CD+FR+E  VEEH+   G++ KK TKTLMF EGCP  L CTILL+G   + LK+VK V
Sbjct: 369  KHCDSFRIEKIVEEHNAA-GESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKEV 428

Query: 623  VQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNPSNIDRSISAIPGFTGPPP 682
            VQY+   AYHL LE  FLAD       +  K   +             +  I  F+  P 
Sbjct: 429  VQYSFILAYHLMLEASFLADRHTMFSTIFAKEATS------------CVVEIENFSPSPS 488

Query: 683  VKES-SIIGDNVVKLDYSEPTL---SQLKYNATSTSSSFLKHDVS-TAYHYMLSSKFASE 742
             +ES S   D  V   + E T+    +       T  S   H  S   Y+ ++ + F+S 
Sbjct: 489  PRESPSEAVDIPVSNGFDEQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSL 548

Query: 743  ASRSLDIKECFMGMTTIKEEAGVDDDTISSCSGIGEALSQCGNSCYISSNSLDGNTHSFI 802
            ++R       ++G     E   V  DT  S +   +++ +       ++   +      +
Sbjct: 549  SAR----LSKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEED---TAEKNEDKQPLLL 608

Query: 803  DLATLRPESTKANLVTKSSREDFPLSPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYG 862
            D   L   S+  +   KS  E+   S  + Q+ILV +S R   +G +CD+ H   IK+Y 
Sbjct: 609  D-PELPVNSSSDDGDNKSQTENDIESTLESQSILVLVSKRNALRGIMCDQRHFSHIKFYK 668

Query: 863  SFDKPLGRFLRDHLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLP-DFFLPGEPE 922
             FD PL +FLRD +F+Q   C +C    EAH++ Y H    LTI  K++P    L GE +
Sbjct: 669  HFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKGLAGEAK 728

Query: 923  GKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFL 982
            GKIWMW RC KC   N    +T+RV++S AA  LSFGKFLELSFS     +R + CGH  
Sbjct: 729  GKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQTFLNRSSSCGHSF 788

Query: 983  HRDCLRFYGFGRTVACFHYAAINVQSVHIPPSKLEFH-HNNRDWIYKEANEVHNRAKSLF 1042
              D L F+G G  VA   Y+ +   +V +PP KLE        W+ KE   V  +  SLF
Sbjct: 789  DSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQTVFTKGISLF 848

Query: 1043 AEVCNALPQIPKNESGSEQEDLGVNELKHYIQELEQICNMEKEDFEVHFKEVLSGETIVG 1102
             +    L ++    + S   DL     +  +  +E++   E+  FE + K        + 
Sbjct: 849  EDAAGFLKRLRSQFTNS---DLRYQRARKLLSNIEELLKHERCIFEENIKNSFDKAKTID 908

Query: 1103 QPAVDILEINKLHMHVLFLSYIWVQRLSFAATLRNHNSLDSLSNDIPTLEERRLDSVEE- 1162
              +  +L +N++   +L  + IW  R            L SL      L +R L S +E 
Sbjct: 909  DVSHRLLRLNRMRWELLLQALIWNYR------------LQSL-----VLSDRLLPSSDET 968

Query: 1163 -LVELNLASRQDEFFSGCDNDSLPSDSKHEIDLGKGGNSGQINQSNEMEREKNIDLG--- 1222
             + E  L +  +   +  +ND+  SDS         G++G I+    +   K+I +    
Sbjct: 969  KIYEQGLKTVSEAGMTRYENDNKVSDS---------GSNGGID--TPLVEHKDIPIAGAS 1028

Query: 1223 -GQEDELCLSPVNSEPLDVGITIGKAMSMGEYPIMDNLSGTLDAVWKAETHPNSVTSEES 1282
             G  D++  S V  +  +   T+  +      PI ++           +TH        +
Sbjct: 1029 VGDNDQMAESYVPED--NESQTLCSSSPDTTSPINNHF----------DTHLAVNVHSTN 1088

Query: 1283 GSQTNSAILEPLAGHS-DFEKCIAN----LVGVEAAQLLHSALVLRRVDTVETCTSSMSM 1342
            G + + +I  P+ G S D E   +N    L   E   L    L  +R+  +E        
Sbjct: 1089 GQEADKSI--PVTGESLDDEVSTSNGPHILGWDEWFWLPFEELRSKRIVDIE-------- 1148

Query: 1343 SSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQGHLRLLMRVGANDTVIPVYDDEPT 1402
                Y  K   +N       ++    + +  ++  +   RL + +  +D ++  Y+DE +
Sbjct: 1149 --KEYLLKFEYVN-------NFTQENLQTVNQIITEESSRLRISLRDDDFIVSDYEDELS 1208

Query: 1403 SIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSANLNSLNSFKDAVSNAYKSHSSN 1462
            S+IA +L      A ++  E          S  P       SL  F D         S  
Sbjct: 1209 SLIACAL------AHLNNEE----------SKKPLSRCIHGSLQGFLD-----NNQDSKQ 1268

Query: 1463 EESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLGQVKYTITCYFAKRFEALRKKCC 1522
             +  +S   S+S    E L   + L   V+F +   +G+ KY+I   +A  F  LRK+CC
Sbjct: 1269 TDRDVSRFSSESTNRLETLPPPEVL---VTFGSVKSVGKPKYSIVSLYADDFRDLRKRCC 1328

Query: 1523 PSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFATAYFKYLS 1582
             SELDY+ SLSRCK W A+GGKS   FAKTLD+RFI+K++ K E ESF+ FAT YFKY+ 
Sbjct: 1329 SSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATEYFKYMK 1388

Query: 1583 EAIRTGCPTCLAKVVGIYQVSSKHLK-GGKESKTDVLVMENLLFGHNVTRLYDLKGSCRS 1642
            ++   G  TCLAKV+GI+QV+ +  K GGKE + D++VMENL F   VTR YDLKG+  +
Sbjct: 1389 DSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDLKGALHA 1448

Query: 1643 RYN-SDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLERAVWNDTSFLASIDVMDYSLL 1702
            R+  +  +G++ VLLDQN +  M  SP++V+  +K+ L+RAV+NDTSFL SI+VMDYSLL
Sbjct: 1449 RFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQRAVYNDTSFLTSINVMDYSLL 1450

Query: 1703 VGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGPKNASPTVISPHQYKKRFRKA 1750
            VG+D+E  ELV GIID++RQYTWDK LETWVK+S ++  PKN  PTVISP  YK RFRK 
Sbjct: 1509 VGVDDENHELVCGIIDYLRQYTWDKQLETWVKSSLVV--PKNVQPTVISPIDYKTRFRKF 1450

BLAST of Sgr019899 vs. ExPASy Swiss-Prot
Match: Q9Z1T6 (1-phosphatidylinositol 3-phosphate 5-kinase OS=Mus musculus OX=10090 GN=Pikfyve PE=1 SV=3)

HSP 1 Score: 416.8 bits (1070), Expect = 1.3e-114
Identity = 514/2120 (24.25%), Postives = 835/2120 (39.39%), Query Frame = 0

Query: 8    LSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRRCGRVFCS 67
            LS ++  +K  M  K   +++ + +WMPD  C+ CYDC  +FT   RRHHCR CG++FCS
Sbjct: 131  LSTVLKRLKEIMEGKSQDSDL-KQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCS 190

Query: 68   KCTANFIPAPSIDSTTAREDSEKIRVCNYCFK-----QWGKEPSSVQNDDNAVS------ 127
            +C    IP   +  T        +R C YC K         + +S+  D NA+S      
Sbjct: 191  RCCNQEIPGKFMGYT------GDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSV 250

Query: 128  SAISQSPSSTSLSSTKSGY-----------------TCHSAISN---------------- 187
            S +  S   T + S K+                   + +SA+S                 
Sbjct: 251  SILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARN 310

Query: 188  -----------------IASTPCSSGPRYHDPYCSGAITDEQDNLL-------------- 247
                             + S   S  P+ +  Y     T+++  +L              
Sbjct: 311  RSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKIC 370

Query: 248  ---------------------------------NGKCINRGTSLMTSSSSYYGYYRSDDE 307
                                             NG    R  ++    +   G +     
Sbjct: 371  HHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVS 430

Query: 308  DDDYYYGIYRSDSESRHISQSDDYYGAVNTDIDHI-AVQGL------------NLDRENI 367
              D    ++R +       QS ++    + D D + +V+G             + D E I
Sbjct: 431  HHDQ---LFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQI 490

Query: 368  STKGSSVIFDNQLPG-----ADEESSEDSDENDKGS---ECDFPTYCMQNKDNEPMDFQN 427
            + +G   + ++  P      +  +S+ DSD     S   E D   + ++     P     
Sbjct: 491  AEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYP----- 550

Query: 428  NGLLWLPPEPEDEEDE-----------------KALLFDDDDDSCAPGEWGYLPSSFGSG 487
                 +PP P D+++                  K  LF+   +  +  E  + P  +   
Sbjct: 551  ----HVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSK-ELPFTPLGWHHN 610

Query: 488  EYPMNGRPNEEYKKTAK--NVVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWE 547
               +    NEE +   +  +    H  AL+ QLL+ E+L          W DII  L  +
Sbjct: 611  NLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESL-------SSSWRDIIVSLVCQ 670

Query: 548  AAAVLKPDTS-KSGGMDPGGYVKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPR 607
                ++PD   +   MD   +V +K + GGK+ +S+VV G +C KN+AH++M   I  P+
Sbjct: 671  VVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPK 730

Query: 608  FFILGGALEY-QRVSNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 667
              +L  ++EY  R     +  D ++ QE + LK  V +I    P ++LVEK+VSR AQ+ 
Sbjct: 731  ILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDM 790

Query: 668  LLSKNITLVLNIKRSLLERIACCTGANIIPSVDHVASL-KLGYCDAFRVETFVEEHDGGD 727
            LL   ITLV+N+K  +LERI+  T  +++ S+D + +   LG C  F ++ F        
Sbjct: 791  LLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIF-------- 850

Query: 728  GQASKKLTKTLMFFEGCPKPLCCTILLRGASEDELKQVKHVVQYATFAAYHLALETCFLA 787
             Q   + TKTLMFFEGCP+ L CTI LRG S+ EL +VK ++ +    AYH  LE  FL 
Sbjct: 851  -QLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLM 910

Query: 788  DEGASVPELPMKSPITVALPHNPSNIDRSISAIPGFTGP--PPVKESSIIGDNVVKL--- 847
            DE A  P L M+SP    L         S   + G + P  P     ++  ++   L   
Sbjct: 911  DEFAMPPTL-MQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESR 970

Query: 848  -----------------------DYSEPTL------------------------------ 907
                                   DY+ PT                               
Sbjct: 971  TVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNE 1030

Query: 908  -------SQLKYNATSTSSSFLKHDVSTAYHYMLSSKFASEASRSL----------DIKE 967
                   +  + +  S   +F          Y+     +SE  R            D+  
Sbjct: 1031 QPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVIL 1090

Query: 968  CFMGMTTIKE-----EAGVDDDT--------------------------------ISSCS 1027
            C   + T +E     E G+   T                                +S   
Sbjct: 1091 CISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQ 1150

Query: 1028 GIGEAL-------------------SQCGNS--------------CYISSNSLDGNTHSF 1087
            G+  ++                      G+S                I S  LD   HS 
Sbjct: 1151 GMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSK 1210

Query: 1088 IDLATLRPESTKANLVTKSSRED--------FP-----LSPSDHQNILVSLSTRCVWKG- 1147
                T      K+   T+S  E         +P     L+P++HQ + V  S+       
Sbjct: 1211 DASCT---SGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSN 1270

Query: 1148 --TICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHS--CGMPSEAHVHCYTHPQGS 1207
              + C    ++ +++YG  D  LG FL  + F  +Y+C S  C  P   H+  + H QG 
Sbjct: 1271 APSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGC 1330

Query: 1208 LTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLEL 1267
            + I  K+L D  +PG  +  I  +  C  C +       T  V +S+ +W +SF K+LEL
Sbjct: 1331 VQIILKEL-DSPVPGY-QHTILTYSWCRICKQ------VTPVVALSNESWSMSFAKYLEL 1390

Query: 1268 SFSNHAAASRVAG--CGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSKLEFHHNN 1327
             F  H    R     CGH +H D  +++ + + VA F Y+ I +  V +P  K+      
Sbjct: 1391 RFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKI------ 1450

Query: 1328 RDWIYKEAN---EVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNELKHYIQELEQIC 1387
              +I ++A     +    K  F +V                          Y+   E++ 
Sbjct: 1451 --FIKRQAPLKVSLLQDLKDFFQKVSQV-----------------------YLAVDERLA 1510

Query: 1388 NMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRLSFAATLRNHNS 1447
            +++ + F    +E +  E I  Q  ++  E              W +++       + ++
Sbjct: 1511 SLKTDTFSKTREEKM--EDIFAQKEMEEGEFKN-----------WTEKMQARLMSSSVDT 1570

Query: 1448 LDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKHEIDLGKGGNSG 1507
               L +   +L  ++    E L   N  SR  + F        PS       L +G  S 
Sbjct: 1571 PQQLQSIFESLIAKKQSLCEVLQAWN--SRLQDLFQQEKGRKRPSVPPSPGRLRQGEES- 1630

Query: 1508 QINQSNEMEREKNIDL-GGQEDELCLSPVNSEPLDVGITIGKAMSMGEYPIMDNLSGTLD 1567
            +IN  +   R  +  L  G++++  L+ ++S+                       S T  
Sbjct: 1631 KINAMDTSPRNISPGLHNGEKEDRFLTTLSSQ-----------------------SSTSS 1690

Query: 1568 AVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAAQLLHSALVLRRV 1627
               +  T P ++  +  G  T+   L+  +G  D      +L+G   +Q       ++  
Sbjct: 1691 THLQLPTPPEALAEQVVGGPTD---LDSASGSEDVFD--GHLLGSTDSQ-------VKEK 1750

Query: 1628 DTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQGHLRLLMRVGAN 1687
             T++   +++   +S YN       +P P    +DP       E E+             
Sbjct: 1751 STMKAIFANLLPGNS-YNP------IPFP----FDPDKHYLMYEHER------------- 1810

Query: 1688 DTVIPVYDDEPTSIIAYSLVMPEYHAQMSE----------------------------PE 1747
               I V + EP+SIIA++L   EY   + E                            P 
Sbjct: 1811 -VPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPI 1870

Query: 1748 RV--IEAGDTTRS--SPPHDSANLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQ-- 1750
            R+  I  G T R+  + P  +   + + SF    +      SS +  TL  A S   Q  
Sbjct: 1871 RLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVG 1930

BLAST of Sgr019899 vs. ExPASy TrEMBL
Match: A0A6J1DUT5 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Momordica charantia OX=3673 GN=LOC111024654 PE=4 SV=1)

HSP 1 Score: 3066.6 bits (7949), Expect = 0.0e+00
Identity = 1542/1799 (85.71%), Postives = 1622/1799 (90.16%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPW+ DPANVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCSKCTANFIPAP ID+  AR+DS+KIRVCN+CFKQWGKEPS+ Q+ DNA SSA+S
Sbjct: 61   CGRVFCSKCTANFIPAPPIDTANARDDSDKIRVCNFCFKQWGKEPSAAQDGDNATSSALS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
             S SS+S+SSTKSGYTCHSAISNIASTPCS+  RY+DP+CSG I+DEQD+ +NGK INR 
Sbjct: 121  LSSSSSSMSSTKSGYTCHSAISNIASTPCSTDRRYYDPFCSGGISDEQDSSINGKSINRA 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTD-IDHIAVQGL 240
            +S+MT+SSS+YGYYRSDDEDDDYYYG+YRSDSESRHISQSDDYY AVN D +D IA Q L
Sbjct: 181  SSMMTTSSSFYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNPDEMDRIAAQSL 240

Query: 241  NLDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNN 300
            NLDREN STKGS  IF+ Q PG +E+SSEDSDENDKGSECDFPTY MQNKDNEP+DFQNN
Sbjct: 241  NLDRENTSTKGSPEIFETQRPGINEKSSEDSDENDKGSECDFPTYAMQNKDNEPVDFQNN 300

Query: 301  GLLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKN 360
            G+LWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSS   GE PM GR +EEY+KTAKN
Sbjct: 301  GILWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSLAGGECPMKGRSSEEYRKTAKN 360

Query: 361  VVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGY 420
            VVEGHFRALVGQLLEAENLP+GDE +KDGWLDIITYLSWEAA VLKPDTSKSGGMDPGGY
Sbjct: 361  VVEGHFRALVGQLLEAENLPLGDEPDKDGWLDIITYLSWEAATVLKPDTSKSGGMDPGGY 420

Query: 421  VKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFD 480
            VKVKCVAGGK SESIVVKGVICKKNVAHRRMTR ISKPRFF+LGGALEYQRV+NHLSSFD
Sbjct: 421  VKVKCVAGGKCSESIVVKGVICKKNVAHRRMTRTISKPRFFVLGGALEYQRVTNHLSSFD 480

Query: 481  TLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIAC 540
            TLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIAC
Sbjct: 481  TLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIAC 540

Query: 541  CTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCC 600
            CTGANI+PSVDHVASLKLGYCDAFRVETFVEEHDG DGQA KK  KTLMFFEGCPKPLCC
Sbjct: 541  CTGANIVPSVDHVASLKLGYCDAFRVETFVEEHDGVDGQAGKKFAKTLMFFEGCPKPLCC 600

Query: 601  TILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNP 660
            TILLRGAS DELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+KSPITVALPHNP
Sbjct: 601  TILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPITVALPHNP 660

Query: 661  SNIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTL 720
            S+IDRSISAIPGFTGP P K                     +SSI   NV+K +  EPTL
Sbjct: 661  SSIDRSISAIPGFTGPSPAKSPEPEPSMEIWQCGKDAVLTRQSSIGCPNVIKSNSLEPTL 720

Query: 721  SQLKYNATSTSSSFLKHDVSTAYHYMLSSKFASEASRSLDIKECFMGMTTIKEEAGVDDD 780
              LK NATSTS S    DVS AY+YMLSSKFASEASRSLDIKEC MGM  IKEEAG DDD
Sbjct: 721  RPLKSNATSTSLSSFDQDVSIAYNYMLSSKFASEASRSLDIKECSMGMMAIKEEAGADDD 780

Query: 781  TISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKANLVTKSSREDFPLS 840
            T    SGIGE LSQ GNS YISSNSLDG   S+IDLA L+PEST +NLVTKSSREDFPLS
Sbjct: 781  T---SSGIGETLSQGGNSSYISSNSLDGTIQSYIDLACLKPESTSSNLVTKSSREDFPLS 840

Query: 841  PSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCGM 900
            PSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCGM
Sbjct: 841  PSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCGM 900

Query: 901  PSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIM 960
            PSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIM
Sbjct: 901  PSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIM 960

Query: 961  SDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSV 1020
            SDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSV
Sbjct: 961  SDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSV 1020

Query: 1021 HIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNELK 1080
            H+PPS LEF+HNN DWI KEA EVHNRA+ +FA VC ALPQI KNESG EQEDLGVNEL 
Sbjct: 1021 HLPPSILEFYHNNLDWINKEATEVHNRAEIIFAGVCKALPQISKNESGIEQEDLGVNELN 1080

Query: 1081 HYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRLS 1140
             YIQELEQI +MEKEDFEVH KEVLSGET V QPAVDILEINKLHMHVL+LSYIWVQRLS
Sbjct: 1081 QYIQELEQIISMEKEDFEVHLKEVLSGETRVYQPAVDILEINKLHMHVLYLSYIWVQRLS 1140

Query: 1141 FAATLRNHNSLDSLSNDIPTLEERRLDSVEELV-ELNLASRQDEFFSGCDNDSLPSDSKH 1200
            FAATLRN NS DSLSNDIP     RLDSVEELV  LNLAS QDEFF GCDNDSLPSDSKH
Sbjct: 1141 FAATLRNKNSQDSLSNDIP-----RLDSVEELVAALNLASGQDEFFVGCDNDSLPSDSKH 1200

Query: 1201 EIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
            EIDLGKGGNS QINQSNEME EK++DLGGQEDE CLS V+SEPL VGITI +A+S+GEYP
Sbjct: 1201 EIDLGKGGNSVQINQSNEMESEKHMDLGGQEDEFCLSRVDSEPLGVGITIERAISVGEYP 1260

Query: 1261 IMDNLSGTLDAVWKAETHPNSVTSEESG-SQTNSAILEPLAGHSDFEKCIANLVGVEAAQ 1320
            IM +LS TLDAVWK   HPNS TSEES  S+TNS ILEPLAG SD EKC ANLVGVEAAQ
Sbjct: 1261 IMSDLSDTLDAVWKGAVHPNSGTSEESNDSRTNSGILEPLAGQSDMEKCTANLVGVEAAQ 1320

Query: 1321 LLHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQG 1380
            LLHSALVLR VD++ET   SMSM SS YN+KISLLNVPKPE +DYDPAFISSFRELEKQG
Sbjct: 1321 LLHSALVLRSVDSIET---SMSMPSSAYNTKISLLNVPKPEFSDYDPAFISSFRELEKQG 1380

Query: 1381 HLRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDS 1440
            HLR LM VG NDT+IPVYDDEPTSI+AYSLV+PEYHAQMSEPERV+EAGDT RS P  DS
Sbjct: 1381 HLRFLMHVGVNDTIIPVYDDEPTSIVAYSLVIPEYHAQMSEPERVMEAGDTARSLPLLDS 1440

Query: 1441 ANLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPL 1500
            A   SL SF   VS+ YKS SSNEE+ L   R+QSIQFE+LLLYTKDLHTRVSF+ ESPL
Sbjct: 1441 ATTESLRSFDQTVSDTYKSLSSNEETMLE--RTQSIQFEDLLLYTKDLHTRVSFVKESPL 1500

Query: 1501 GQVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFII 1560
            GQVKYTITCYFAKRFEALRKKCCPSELDYV+SLSRCKRWGAQGGKSNVFFAKTLDERFII
Sbjct: 1501 GQVKYTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFII 1560

Query: 1561 KQVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLV 1620
            KQVTKIELESFIQF  +YFKYLSEAIRTGCPTCLAKVVG+YQVSSKHLKGGKESKTDVLV
Sbjct: 1561 KQVTKIELESFIQFGPSYFKYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKESKTDVLV 1620

Query: 1621 MENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLE 1680
            MENLLF HNVTRLYDLKGS RSRYNSDTSGKNKVLLDQNLIESMPT PIF+ SKAKRLLE
Sbjct: 1621 MENLLFRHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTCPIFLGSKAKRLLE 1680

Query: 1681 RAVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGG 1740
            RAVWNDTSFLASIDVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGILGG
Sbjct: 1681 RAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGG 1740

Query: 1741 PKNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENSWD 1776
            PKNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDEN EH++SWD
Sbjct: 1741 PKNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEHDSSWD 1786

BLAST of Sgr019899 vs. ExPASy TrEMBL
Match: A0A1S4DVR6 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103487265 PE=4 SV=1)

HSP 1 Score: 2888.6 bits (7487), Expect = 0.0e+00
Identity = 1459/1798 (81.15%), Postives = 1566/1798 (87.10%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENK+SDLVGLVKSWMPW+ DPANVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCSKCTANFIPA S D T AREDSEKIRVCNYCFKQWGKEPSS+Q+ D A SSA+S
Sbjct: 61   CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
             S SSTS+ STKSGYTCHSAIS+IASTPCS+  +Y+DPYCS AI DE DNL NGK INR 
Sbjct: 121  VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTDIDHIAVQGLN 240
            TSLMTSSSSY GYYRSDDEDDDYYYG+YRSDSESRHISQSDDYY AVN D       GLN
Sbjct: 181  TSLMTSSSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNID------GGLN 240

Query: 241  LDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNNG 300
            L+REN STKGS V FD Q P  +EESSEDSDENDKGSECDFPTY MQNKD+EPMDFQNNG
Sbjct: 241  LERENTSTKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNG 300

Query: 301  LLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKNV 360
            +LWLPPEPEDEEDEKALLF+DDDD CAPGEWG+L SS  SGE PM G+ +E+YKKTAKNV
Sbjct: 301  ILWLPPEPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNV 360

Query: 361  VEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420
            VEGHFRALV QLLEAENLPIGD  N+DGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV
Sbjct: 361  VEGHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420

Query: 421  KVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFDT 480
            KVKCVAGGKRSESI+VKG+ICKKNVAHRRMTR ISKPRFF+LGGALEYQRV+NHLSSFDT
Sbjct: 421  KVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDT 480

Query: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540
            LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC
Sbjct: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540

Query: 541  TGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCCT 600
            TGANIIP+VDHVASLKLGYCDAFRVETFVEEHD GDGQ  KK TKTLMFFEGCPKPLCCT
Sbjct: 541  TGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 600

Query: 601  ILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNPS 660
            ILLRGAS DELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+ SPITVALPHNPS
Sbjct: 601  ILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPS 660

Query: 661  NIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTLS 720
            NIDRSISAIPGFT P PVK                     + SII  NV  L+  EPTLS
Sbjct: 661  NIDRSISAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLS 720

Query: 721  QLKYNATSTSSSFLKHDVSTAYHYMLSSKFASEASRSLDIKECFMGMTTIKEEAGVDDDT 780
             L+ NATSTS SFLK D ST    MLSSKF SE  RSLD KE  MGM TIKEEA  D D 
Sbjct: 721  LLESNATSTSFSFLKQDFST----MLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDP 780

Query: 781  ISSCSGIGEALSQCGNS-CYISSNSLDGNTHSFIDLATLRPESTKANLV-TKSSREDFPL 840
            +SS SG  EA S+  NS   +S NSLD NT S  D  +  PE++  +LV TKSSREDFPL
Sbjct: 781  VSS-SGKREASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPL 840

Query: 841  SPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCG 900
            SPSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRD+LFDQNYRCHSCG
Sbjct: 841  SPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCG 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960
            MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI
Sbjct: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960

Query: 961  MSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020
            MSDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS
Sbjct: 961  MSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020

Query: 1021 VHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNEL 1080
            VHIPPS +EFH NN +WI KEANEVHNRAK+LF EVC ALP I KNESG+EQED G +EL
Sbjct: 1021 VHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADEL 1080

Query: 1081 KHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRL 1140
             +YIQELEQI NMEKE+FEVHF +VLSG T V QP VDI EINKLHMH+LFLSYIWVQRL
Sbjct: 1081 NNYIQELEQIINMEKENFEVHFNKVLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRL 1140

Query: 1141 SFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKH 1200
            ++ ATLR  +S D  SNDI TLEE+ L+S+EELVEL LAS +DEFF GCDNDSLP +SK 
Sbjct: 1141 NYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPESKR 1200

Query: 1201 EIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
            E  LG+ GNSG I++ NE  REKNIDLGGQED+ CLSPVNSEP+DVGI I +A S+GEYP
Sbjct: 1201 EAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVGEYP 1260

Query: 1261 IMDNLSGTLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAAQL 1320
            IM +LSGTLDAVWK   HPNS+  E+S S+TNSAILEPLAG SD EKC AN  G+E A L
Sbjct: 1261 IMTDLSGTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTAN--GIETAHL 1320

Query: 1321 LHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQGH 1380
            LHSALVL   D+VET T+S SM  S YN+KISLLN+PK +I+DY   FISS+RELEKQ H
Sbjct: 1321 LHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSH 1380

Query: 1381 LRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSA 1440
            LRLLM VG++DTV+PVYD+EPTSIIAYSL+MPEY AQMSEP + +EA DT RS P   S 
Sbjct: 1381 LRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGSV 1440

Query: 1441 NLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLG 1500
             +NS+ +  +  S+AYK+  SNEE+ LSM R++S+QFE+L+  TKDLHTRV F+NE+PLG
Sbjct: 1441 KMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPLG 1500

Query: 1501 QVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560
             VKYTITCYFAKRFEALRKKCCPSELD+V+SLSRC+RWGAQGGKSNVFFAKTLDERFIIK
Sbjct: 1501 PVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIK 1560

Query: 1561 QVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVM 1620
            QVTKIELESFIQF+ AYF YLS+AI TGCPTCLAKVVG+YQVSSKHLKGGKE K DVLVM
Sbjct: 1561 QVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVM 1620

Query: 1621 ENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLER 1680
            ENLLF HNVTRLYDLKGS RSRYN DTSG NKVLLDQNLIESMPTSPIF+ SKAKRLLER
Sbjct: 1621 ENLLFRHNVTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKAKRLLER 1680

Query: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGP 1740
            AVWNDTSFLASIDVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGILGGP
Sbjct: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGP 1740

Query: 1741 KNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENSWD 1776
            KNA+PTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDEN E ENSWD
Sbjct: 1741 KNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPENSWD 1785

BLAST of Sgr019899 vs. ExPASy TrEMBL
Match: A0A6J1H961 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111461703 PE=4 SV=1)

HSP 1 Score: 2867.0 bits (7431), Expect = 0.0e+00
Identity = 1442/1798 (80.20%), Postives = 1570/1798 (87.32%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPW+ DP NVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCSKCTANFIP+PS D T AREDSE IRVCNYCFKQWGKEPS++ + D A  SAIS
Sbjct: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
             S SSTS+ STKSGYTC SAISNI STPCS+  +Y+DP+CSG+I DEQD+L NGK I R 
Sbjct: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTDIDHIAVQGLN 240
            TSLMTSSSSYYGY RSDDED DYYYG+ RSDSESRHI QSD YY + NT+      +GLN
Sbjct: 181  TSLMTSSSSYYGYCRSDDEDGDYYYGMCRSDSESRHIPQSDHYYCSANTE------EGLN 240

Query: 241  LDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNNG 300
            L+REN+STKGS V FD Q P  DEESSEDSDENDKGSECDFPTY MQNKDNE MDFQNNG
Sbjct: 241  LERENMSTKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNG 300

Query: 301  LLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKNV 360
            +LWLPPEPEDEEDEKALLF+ DDD+CAPGEWG+LPSS GSGE PM  + + +Y++TAKNV
Sbjct: 301  ILWLPPEPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNV 360

Query: 361  VEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420
            VEGHFRALV QLLEAENLP+GDE N+DGWLDI+TYLSWEAAAVLKPDTSKSG MDPGGYV
Sbjct: 361  VEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYV 420

Query: 421  KVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFDT 480
            KVKCVAGGKR+ESI+VKG+ICKKNVAHRRM ++ISKPRFF+LGGALEYQRV+NHLSSFDT
Sbjct: 421  KVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDT 480

Query: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540
            LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSK ITLVLN+KRSLLERIACC
Sbjct: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACC 540

Query: 541  TGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCCT 600
            TGANI+P+VDHVA LKLGYCD FRV+TFVEEH  GDGQA KK TKTLMFFEGCPKPLCCT
Sbjct: 541  TGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCT 600

Query: 601  ILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNPS 660
            ILLRGAS DELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+KSPI V LPH+PS
Sbjct: 601  ILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPS 660

Query: 661  NIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTLS 720
            NIDRSIS IPGFT P PVK                     +SS+I  NV  L+  EP+L 
Sbjct: 661  NIDRSISTIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLR 720

Query: 721  QLKYNATSTSSSFLKHDVSTA-YHYMLSSKFASEASRSLDIKECFMGMTTIKEEAGVDDD 780
             +K NATSTS SFLK +VSTA  +YMLSSK ASEA+R+ D +E  MG+ TIKEEA  DDD
Sbjct: 721  LVKSNATSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDD 780

Query: 781  TISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKANLV-TKSSREDFPL 840
            T+SS SG G  LS  GNS +I S+SLD NT S  D    RPES  ANLV TKSSREDFPL
Sbjct: 781  TVSS-SGKGVPLSH-GNSIHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPL 840

Query: 841  SPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCG 900
            SPSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRD+LFDQNYRCHSCG
Sbjct: 841  SPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCG 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960
            MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI
Sbjct: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960

Query: 961  MSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020
            MSDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINV+S
Sbjct: 961  MSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVKS 1020

Query: 1021 VHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNEL 1080
            VHIPPS ++FHHNN +WI KEANEVHNRA+ LFA VC  LP I K+ESG+EQED GVNEL
Sbjct: 1021 VHIPPSIIDFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNEL 1080

Query: 1081 KHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRL 1140
             +YIQELEQI N+EKEDFEVHF +  SGET VGQP VDI EINKLHMHVLFLSYIWVQRL
Sbjct: 1081 NNYIQELEQIINVEKEDFEVHFNKA-SGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRL 1140

Query: 1141 SFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKH 1200
            S+A TL   +S DS SNDIPTLEE+ LDS+EELVEL LA   D+ F G DNDSLPS+SKH
Sbjct: 1141 SYAVTLGKKHSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKH 1200

Query: 1201 EIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
            ++DL +GGNSGQ+N+ NE EREKNIDLGGQED+LCL  VNSEPLDVG  + +  S GEYP
Sbjct: 1201 DVDLDRGGNSGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYP 1260

Query: 1261 IMDNLSGTLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAAQL 1320
             M +LSGTLDAVWK   HPNS+  ++   QT+S+ILEPLAG  D EKC AN  GVE A+L
Sbjct: 1261 SMTDLSGTLDAVWKGALHPNSMLFDDRYPQTSSSILEPLAG-PDMEKCTAN--GVETARL 1320

Query: 1321 LHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQGH 1380
            L+SALVL  VD+VETCTS  SM SS Y+SKISLLN+PKP+I+DYDPA I+SFRELEKQGH
Sbjct: 1321 LYSALVLTGVDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGH 1380

Query: 1381 LRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSA 1440
            LRL M V ++DTV+PVYD+EPTSIIAYSL+MPEYHAQMSEPERV+EA DT RS P   S 
Sbjct: 1381 LRLFMHVDSSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSV 1440

Query: 1441 NLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLG 1500
             ++S+ +  +  S+ Y++  SNEE+ LSMAR++S+QFE+LLL+TKDLHTRV F+NE+PLG
Sbjct: 1441 KMSSIKTCDEEGSDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLG 1500

Query: 1501 QVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560
             VKYTITCYFAKRFEALRKKCCPSELDYV+SLSRCKRWGAQGGKSNVFFAKTLDERFIIK
Sbjct: 1501 PVKYTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560

Query: 1561 QVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVM 1620
            QVTKIELESFIQFA AYF YLSE+IRTGCPTCLAKVVG+YQVS+KHLKGGKE K DVLVM
Sbjct: 1561 QVTKIELESFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVM 1620

Query: 1621 ENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLER 1680
            ENLLFGHNVTRLYDLKGS RSRYNSDTSGKNKVLLDQNLIESMPTSPIF+  KAKRLLER
Sbjct: 1621 ENLLFGHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLER 1680

Query: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGP 1740
            AVWNDTSFLASIDVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGILGGP
Sbjct: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGP 1740

Query: 1741 KNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENSWD 1776
            KNA+PTVISPHQYKKRFRKAMTTYFLMVPDQWPSST+SPSESHPDLCDEN E ENSWD
Sbjct: 1741 KNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTISPSESHPDLCDENSEPENSWD 1786

BLAST of Sgr019899 vs. ExPASy TrEMBL
Match: A0A0A0M3A5 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_1G662800 PE=4 SV=1)

HSP 1 Score: 2862.8 bits (7420), Expect = 0.0e+00
Identity = 1440/1798 (80.09%), Postives = 1564/1798 (86.99%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENK+SDLVGLVKSWMPW+ DP NVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCS+CTANFIPAPS D+T AREDSEKIRVCNYCFKQWGKEPSS+Q+ D A SSA+S
Sbjct: 61   CGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
             S SSTS+ STKSGYTCHSAIS+IASTPCS+G +Y+DPYCS A+ DE DNL NGK INR 
Sbjct: 121  LSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTDIDHIAVQGLN 240
            TSLMTSSSSYYGYYRSDDEDDDYYYG+YRSDSESRHISQSDDYY AVN D      +G N
Sbjct: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNID------EGSN 240

Query: 241  LDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNNG 300
            L+REN STKGS V FD Q P  +EESSEDSDENDKGSECDFP+Y MQNK++EPMDFQNNG
Sbjct: 241  LERENTSTKGSPVNFDTQSPRGNEESSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNG 300

Query: 301  LLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKNV 360
            +LWLPPEPEDEEDEKALLF+DDDD CAPGEWGYL SS  SGE PM G+ +E+YKKTAKNV
Sbjct: 301  ILWLPPEPEDEEDEKALLFEDDDDVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNV 360

Query: 361  VEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420
            VEGHFRALV QLLEAENLPIGD   +DGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV
Sbjct: 361  VEGHFRALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420

Query: 421  KVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFDT 480
            KVKCVAGGKRSESI+VKG+ICKKNVAHRRMTR ISKPRFF+LGGALEYQRV+NHLSSFDT
Sbjct: 421  KVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDT 480

Query: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540
            LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC
Sbjct: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540

Query: 541  TGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCCT 600
            TGANIIP+VDHVASLKLGYCDAFRVETFVEEHD GDGQ  KK TKTLMFFEGCPKPLCCT
Sbjct: 541  TGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCT 600

Query: 601  ILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNPS 660
            ILLRGAS DELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+ SPITVALPHNPS
Sbjct: 601  ILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPS 660

Query: 661  NIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTLS 720
            N+DRSISAIPGFT P PVK                     +SSII  NVV L+  EPT S
Sbjct: 661  NVDRSISAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDSSIISHNVVNLNSLEPTSS 720

Query: 721  QLKYNATSTSSSFLKHDVSTAYHYMLSSKFASEASRSLDIKECFMGMTTIKEEAGVDDDT 780
             L+ NATS S SFLK D ST    M+SS F SE  RSL  KE  MGM  IKEEAG DDD 
Sbjct: 721  LLESNATSPSFSFLKQDFST----MISSMFDSEGKRSLYSKEYSMGMMNIKEEAGEDDDP 780

Query: 781  ISSCSGIGEALSQCGNS-CYISSNSLDGNTHSFIDLATLRPESTKANLV-TKSSREDFPL 840
            +SS SG  EA S+  N+   ++ NSLD NT S  +  +  PE+   +LV TKSSREDFPL
Sbjct: 781  VSS-SGKREASSRHANTKVLMNRNSLDANTQSPNNSTSQGPENFNTDLVTTKSSREDFPL 840

Query: 841  SPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCG 900
            SPSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRD+LFDQNYRCHSCG
Sbjct: 841  SPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCG 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960
            MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI
Sbjct: 901  MPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVI 960

Query: 961  MSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020
            MSDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS
Sbjct: 961  MSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQS 1020

Query: 1021 VHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNEL 1080
            VHIPPS +EFH NN +WI KEANEVHNRAK+LF EVC ALP IPKNESG+EQED GV+EL
Sbjct: 1021 VHIPPSMIEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQEDFGVDEL 1080

Query: 1081 KHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRL 1140
             +YIQELEQI NMEKE+FEVHF ++LSG T V QP VDI EINKLHMH+LFLSYIWVQRL
Sbjct: 1081 NNYIQELEQIINMEKENFEVHFNKLLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRL 1140

Query: 1141 SFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKH 1200
            ++ ATLR  +S D  SNDI  L E+ L+S+EELVEL LAS +DEFF GCDNDSLP++SK 
Sbjct: 1141 NYVATLRKKHSPDGSSNDISILVEKELNSLEELVELTLASGRDEFFGGCDNDSLPAESKR 1200

Query: 1201 EIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
            E  LG+ GNSG I++ N+ +REKNIDLGGQED+ CLSPVN +P+DVGI I +A S+GEYP
Sbjct: 1201 EAGLGREGNSGNISRLNKTDREKNIDLGGQEDDFCLSPVNLDPVDVGIGIRRANSVGEYP 1260

Query: 1261 IMDNLSGTLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAAQL 1320
            IM  + GTLDAVWK   HPNS+  ++S S+T SAILEPLAG SD EKC AN   +E A L
Sbjct: 1261 IM-TVPGTLDAVWKGVIHPNSLIFDDSFSRTTSAILEPLAGQSDMEKCTAN--RIETAHL 1320

Query: 1321 LHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELEKQGH 1380
            LHSALVL R D+VET  +S SM  S YN+KISLLN+PK +I+DY   FISS+RELEKQ H
Sbjct: 1321 LHSALVLTRDDSVETIPNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSH 1380

Query: 1381 LRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSA 1440
            LRLLM VG++DTV+PVYD+EPTSIIAYS++MPEY AQMSEP + +EA DT RS P   S 
Sbjct: 1381 LRLLMHVGSSDTVVPVYDEEPTSIIAYSMLMPEYIAQMSEPAKGMEASDTARSLPVIGSV 1440

Query: 1441 NLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLG 1500
             +NS+ +  +  S+AYK+  SNEE+ LSM R++S+QFE+L+  +KDLHTRV F+NE+PLG
Sbjct: 1441 KMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMSKDLHTRVCFINETPLG 1500

Query: 1501 QVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1560
             VKYTITCYFAKRFEALRKKCCPSELD+V+SLSRC+RWGAQGGKSNVFFAKTLDERFIIK
Sbjct: 1501 PVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIK 1560

Query: 1561 QVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVM 1620
            QVTKIELESFIQF+ AYF YLS+AI TGCPTCLAKVVG+YQVSSKHLKGGKE K DVLVM
Sbjct: 1561 QVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVM 1620

Query: 1621 ENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLER 1680
            ENLLF HNVTRLYDLKGS RSRYN DTSGKNKVLLDQNLIESMPTSPIF+ SKAKRLLER
Sbjct: 1621 ENLLFRHNVTRLYDLKGSSRSRYNPDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLER 1680

Query: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGP 1740
            AVWNDTSFLASIDVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGILGGP
Sbjct: 1681 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGP 1740

Query: 1741 KNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENSWD 1776
            KNA+PTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDEN E ENSWD
Sbjct: 1741 KNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPENSWD 1784

BLAST of Sgr019899 vs. ExPASy TrEMBL
Match: A0A6J1JKD8 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111485374 PE=4 SV=1)

HSP 1 Score: 2857.0 bits (7405), Expect = 0.0e+00
Identity = 1442/1802 (80.02%), Postives = 1568/1802 (87.01%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSW+PW+ DP NVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWIPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFCSKCTANFIP+PS D T AREDSE IRVCNYCFKQWGKEPS++ + + A  SAIS
Sbjct: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGNKATCSAIS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAITDEQDNLLNGKCINRG 180
             S SSTS+ STKSGYTC SAISNI STPCS+  +Y+DP+CS +I DEQD+L NGK I R 
Sbjct: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSVSIADEQDSLRNGKTIKRS 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTDIDHIAVQGLN 240
            TSLMTSSSSYYGY RSDDED DYYYG+ RSDSESRHISQS  YY + NT+      +GLN
Sbjct: 181  TSLMTSSSSYYGYCRSDDEDGDYYYGMCRSDSESRHISQSGHYYCSANTE------EGLN 240

Query: 241  LDRENISTKGSSVIFDNQLPGADEESSEDSDENDKGSECDFPTYCMQNKDNEPMDFQNNG 300
            L+REN+STKGS V FD Q P  DEESSEDSDENDKGSECDFPTY MQNKDNE MDFQNNG
Sbjct: 241  LERENMSTKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNG 300

Query: 301  LLWLPPEPEDEEDEKALLFDDDDDSCAPGEWGYLPSSFGSGEYPMNGRPNEEYKKTAKNV 360
            +LWLPPEPEDEEDEKALLF+ DDD+CAPGEWG+LPSS GSGE PM  + + +Y++TAKNV
Sbjct: 301  ILWLPPEPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNV 360

Query: 361  VEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYV 420
            VEGHFRALV QLLEAENLP+GDE N+DGWLDI+TYLSWEAAAVLKPDTSKSGGMDPGGYV
Sbjct: 361  VEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGGMDPGGYV 420

Query: 421  KVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGGALEYQRVSNHLSSFDT 480
            KVKCVAGGKR+ESI+VKG+ICKKNVAHRRMT++ISKPRFF+LGGALEYQRV+NHLSSFDT
Sbjct: 421  KVKCVAGGKRTESIIVKGIICKKNVAHRRMTKEISKPRFFVLGGALEYQRVTNHLSSFDT 480

Query: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACC 540
            LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSK ITLVLN+KRSLLERIACC
Sbjct: 481  LLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACC 540

Query: 541  TGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLTKTLMFFEGCPKPLCCT 600
            TGANI+P+VDHVA LKLGYCD FRV+TFVEEH  GDGQA KK TKTLMFFEGCPKPLCCT
Sbjct: 541  TGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCT 600

Query: 601  ILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPELPMKSPITVALPHNPS 660
            ILLRGAS DELK+VKHVVQYATFAAYHLALETCFLADEGASVPELP+KSPI V LPH+PS
Sbjct: 601  ILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPS 660

Query: 661  NIDRSISAIPGFTGPPPVK---------------------ESSIIGDNVVKLDYSEPTLS 720
            NIDRSISAIPGFT P PVK                     +SSII  NV  L+  EP+L 
Sbjct: 661  NIDRSISAIPGFTCPSPVKSPEPEPSMETGQSGNDAVPTRDSSIISHNVDNLNSLEPSLR 720

Query: 721  QLKYNATSTSSSFLKHDVSTA-YHYMLSSKFASEASRSLDIKECFMGMTTIKEE----AG 780
             +K NATST+ SFLK +VSTA  +YMLSSK ASEA+R+ D +E  MGM TIKEE    A 
Sbjct: 721  LVKSNATSTNLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGMMTIKEEAKEDAK 780

Query: 781  VDDDTISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKANLV-TKSSRE 840
             DDDT+SS SG G  L++ GNS +I SNSLD NT    D    R ES  ANLV TKSSRE
Sbjct: 781  EDDDTVSS-SGKGVPLTR-GNSIHIRSNSLDANTQGSNDSTCQRTESLIANLVTTKSSRE 840

Query: 841  DFPLSPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRC 900
            DFPLSPSD QNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRD+LFDQNYRC
Sbjct: 841  DFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRC 900

Query: 901  HSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPAT 960
            HSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPAT
Sbjct: 901  HSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPAT 960

Query: 961  RRVIMSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI 1020
            RRVIMSDAAWGLSFGKFLELSFSN+AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI
Sbjct: 961  RRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI 1020

Query: 1021 NVQSVHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLG 1080
            NV+SVHIPPS +EFHHNN +WI KEANEVHNRA+ LFAEVC  LP I K+ESG+EQED G
Sbjct: 1021 NVKSVHIPPSIIEFHHNNLEWINKEANEVHNRAEVLFAEVCKTLPLILKHESGTEQEDFG 1080

Query: 1081 VNELKHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIW 1140
            VNEL +YIQELEQI N+EKEDFEVHF +  SGET VGQP VDI EINKLHMHVLFLSYIW
Sbjct: 1081 VNELNNYIQELEQIINVEKEDFEVHFNKA-SGETTVGQPTVDIFEINKLHMHVLFLSYIW 1140

Query: 1141 VQRLSFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPS 1200
            VQRLS+A TL   +S DS SNDIPTLEE+ LDS+EELVEL LA  QD+ F G DNDSLPS
Sbjct: 1141 VQRLSYAVTLGKKHSPDSSSNDIPTLEEKGLDSMEELVELTLAFGQDDLFGGFDNDSLPS 1200

Query: 1201 DSKHEIDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSM 1260
            +SKH++DL +GGNSGQ+N+  E EREKNIDLGGQED+LCL  VNSEPLDVG  + +  S 
Sbjct: 1201 ESKHDVDLDRGGNSGQMNRFYETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSE 1260

Query: 1261 GEYPIMDNLSGTLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVE 1320
            GEY  M +LSGTL AVWK   HPNS+  ++   QTNS+ILEPLAG  D EKC AN  GVE
Sbjct: 1261 GEYSSMTDLSGTLVAVWKGAIHPNSILFDDRYPQTNSSILEPLAG-PDMEKCTAN--GVE 1320

Query: 1321 AAQLLHSALVLRRVDTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELE 1380
               LL+SALVL RVD+VETCTS  SM SS Y+SKISLLN+PKP+I+DYDPA I+SFRELE
Sbjct: 1321 ITHLLYSALVLTRVDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELE 1380

Query: 1381 KQGHLRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPP 1440
            +QGHLRL M VG++DTV+PVYD+EPTSIIAYSL+MPEYHAQMSEPERV+EA DT RS P 
Sbjct: 1381 RQGHLRLFMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPT 1440

Query: 1441 HDSANLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNE 1500
              S  + S+ +  +  S+ Y++  SNEE+ LSMAR++S+QFE+LLL+TKDLHTRV F+NE
Sbjct: 1441 IGSVKMGSIKTCDEEGSDVYRTLGSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINE 1500

Query: 1501 SPLGQVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDER 1560
            +PLG VKYTITCYFAKRFEALRKKCCPSELDYV+SLSRCKRWGAQGGKSNVFFAKTLDER
Sbjct: 1501 TPLGPVKYTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDER 1560

Query: 1561 FIIKQVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTD 1620
            FIIKQVTKIELESFIQFA AYF YLSE+IRTGCPTCLAKVVG+YQV +KHLKGGKE K D
Sbjct: 1561 FIIKQVTKIELESFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVFTKHLKGGKEFKID 1620

Query: 1621 VLVMENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKR 1680
            VLVMENLLFGHNVTRLYDLKGS RSRYNSDTSGKNKVLLDQNLIESMPTSPIF+  KAKR
Sbjct: 1621 VLVMENLLFGHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKR 1680

Query: 1681 LLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGI 1740
            LLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELV+GIIDFMRQYTWDKHLETWVKNSGI
Sbjct: 1681 LLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGI 1740

Query: 1741 LGGPKNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENLEHENS 1776
            LGGPKNA+PTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDEN E ENS
Sbjct: 1741 LGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPENS 1790

BLAST of Sgr019899 vs. TAIR 10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 1697.2 bits (4394), Expect = 0.0e+00
Identity = 965/1818 (53.08%), Postives = 1212/1818 (66.67%), Query Frame = 0

Query: 9    SDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRRCGRVFCSK 68
            S++VGL+KSW+PW+ +PA VSRDFWMPD+SCRVCY+CD QFT INRRHHCR CGRVFC K
Sbjct: 12   SEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGK 71

Query: 69   CTANFIPAPSIDSTTAREDSEKIRVCNYCFKQW--GKEPSSVQNDDNAVSSAISQSPSST 128
            CTAN IP    D  T RED E+IRVCNYCF+QW  G     V N      + +S SPS T
Sbjct: 72   CTANSIPFAPSDLRTPREDWERIRVCNYCFRQWEQGDGGPHVSN-----ITELSTSPSET 131

Query: 129  SLSSTKSGYTCHSAISNIASTPCSSG--PRYH---DPYCSGAITDEQDNLLNGKCINRGT 188
            SL S+K+  T +S+   + S P   G   R H   D    G  + E      GK  +R +
Sbjct: 132  SLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRRS 191

Query: 189  SLMTS-----SSSYYGYYRSDDEDDDYYYGIYRSDSESRHISQSDDYYGAVNTD---IDH 248
            S + +     S       RSDDE D+  YG Y++D E+ H  ++++YYG +  +   ID 
Sbjct: 192  SFIATDVEDPSRFALNSIRSDDEYDE--YGAYQTDIETSHSPRANEYYGPMEYNGMGIDD 251

Query: 249  IAVQGLNLDRENISTKGSSVIFDNQLPGADEESSED---SDENDKGSECD--FPTYCMQN 308
            +  + L  +  +  +   S +    L     E SE      E+D   EC+   P     +
Sbjct: 252  VPCKHLGGETADQKSLSGSPLIHQCLESLIREGSEQFQKKSEHDGRDECEASSPADISDD 311

Query: 309  KDNEPMDFQNNGLLWLPPEPEDEEDEK--ALLFDDDDDSCAPGEWGYL--PSSFGSGEYP 368
            +  EP+DF+NNGLLW+PPEPE+EEDE+  AL  ++D++  A GEWGYL   +SFGSGEY 
Sbjct: 312  QVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSGEYR 371

Query: 369  MNGRPNEEYKKTAKNVVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVL 428
               R  EE+KK  KNVV+GHFRAL+ QLL+ EN+ + DE  K+ WL+IIT LSWEAA +L
Sbjct: 372  GEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANLL 431

Query: 429  KPDTSKSGGMDPGGYVKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGG 488
            KPD SKSGGMDPGGYVKVKC+A G R +S+VVKGV+CKKNV +RRM+ KI K R  ILGG
Sbjct: 432  KPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILGG 491

Query: 489  ALEYQRVSNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNIT 548
             LEYQRVSN LSSFDTLLQQE DHLKMAVAKI A  PN+LLVEKSVSR AQEYLL+K+I+
Sbjct: 492  GLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDIS 551

Query: 549  LVLNIKRSLLERIACCTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLT 608
            LVLNIKR LL+RIA CTGA IIPSVDH++S KLGYC+ FRV+ + EEH G  GQ  KK+ 
Sbjct: 552  LVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEH-GSTGQVGKKVV 611

Query: 609  KTLMFFEGCPKPLCCTILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPE 668
            KTLM+FE CPKPL  TILLRGA+EDELK+VKHVVQY  FAAYHLALET FLADEGAS PE
Sbjct: 612  KTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS-PE 671

Query: 669  LPMKSPITVALPHNPSNIDRSISAIPGFTGPPPVKESSIIGDNVVKLDYSEPTLSQLKYN 728
            LP+ SPITVALP   ++I+RSIS +PGFT     K  +++     +   S P +S+L   
Sbjct: 672  LPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVP-VSELLST 731

Query: 729  ATSTS-----------SSFLKHDVSTAY---HYMLSSKFASEA--SRSLDIKECFMGMTT 788
             T+ S           S +   +++ ++    + +S         SR+ D+    + + T
Sbjct: 732  TTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSGRSVPVDT 791

Query: 789  IKEEAG----VDDDTISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKA 848
              +++      D+ T +S    G+   +   S  I ++ +  N  +  +L   + ++ + 
Sbjct: 792  PADKSNPIVVADETTNNSLHLSGQGFVR--KSSQIGTSIMVENQDNGSELTIAQQQNNEK 851

Query: 849  NLVTKSSREDFPLSPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRD 908
               T+S +E+FP SPSDHQ+ILVSLS+R VWKGT+C+R+HL RIKYYGSFDKPLGRFLRD
Sbjct: 852  PKETQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLRD 911

Query: 909  HLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCP 968
            HLFDQ+YRC SC MPSEAHVHCYTH QGSLTIS KKL D+ LPGE EGKIWMWHRCL+CP
Sbjct: 912  HLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRCLRCP 971

Query: 969  RTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRT 1028
            R NGFPPAT RV+MSDAAWGLSFGKFLELSFSNHAAASRVA CGH LHRDCLRFYGFG  
Sbjct: 972  RLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGNM 1031

Query: 1029 VACFHYAAINVQSVHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNE 1088
            VACF YA I+V SV++PPS L F++ N+DWI +E +EV  RA+ LF+EV NA+ QI   E
Sbjct: 1032 VACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQIA--E 1091

Query: 1089 SGSEQEDLGVNELKHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHM 1148
             G           +  I ELE++   EK +FE + +++L  E   GQP VDILE+ ++H 
Sbjct: 1092 KG----------FRRRIGELEEVLQKEKAEFEENMQKILHREVNEGQPLVDILELYRIHR 1151

Query: 1149 HVLFLSYIWVQRLSFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFS 1208
             +LF SY+W  RL  A+TL    + D    +    E+  L   + L E+N  +  +   +
Sbjct: 1152 QLLFQSYMWDHRLINASTLHKLENSDDTKRE--ENEKPPLAKSQTLPEMNAGT--NSLLT 1211

Query: 1209 GCDNDSLPSDSKHEIDLGKGGNSGQINQSNEMEREKNIDL------GGQEDELCLSPVNS 1268
            G + +  P       D    G++G +N + + E + N DL      GG+       P  S
Sbjct: 1212 GSEVNLNP-------DGDSTGDTGSLN-NVQKEADTNSDLYQEKDDGGEVSPSKTLPDTS 1271

Query: 1269 EPLDVGITIGKAMSMGEYPIMDNLSGTLDAVW------KAETHPNSVTSEESGSQTNSAI 1328
             PL+  + + +  S G+  +M NLS TLDA W        E   N+  S    + +NS+ 
Sbjct: 1272 YPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVEIPTNNKVSLPPSTMSNSST 1331

Query: 1329 LEPLAGHSDFEKCIANLVGVEAAQLLHSALVLRRVDTVETCTSSMSMS-SSVYNS--KIS 1388
              P++               + A  +  AL  +  +  E   S +S+   + Y S  K  
Sbjct: 1332 FPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSEDSVSWLSVPFLNFYRSINKNF 1391

Query: 1389 LLNVPKPE-IADYDPAFISSFRELEKQGHLRLLMRVGANDTVIPVYDDEPTSIIAYSLVM 1448
            LL+  K +   ++ P +ISSFRE E QG  RLL+ VG ND V+PVYDDEPTS+IAY+L+ 
Sbjct: 1392 LLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDIVVPVYDDEPTSMIAYALMS 1451

Query: 1449 PEYHAQMSEPERVIEAGDTTRSSPPHDSANLNSLNSFKDAVSNAYKSHSSNEESTLSMAR 1508
            PEY  Q S        G++  S P    + LN      D + +  +S+ S +ES LS++ 
Sbjct: 1452 PEYQRQTS------AEGESLVSYP----SELNIPRPVDDTIFDPSRSNGSVDESILSISS 1511

Query: 1509 SQSIQFEELLLYTKDLHTRVSFLNESPLGQVKYTITCYFAKRFEALRKKCCPSELDYVKS 1568
            S+S    + L YTK LH RVS+  +  LG+VKYT+TCY+AKRFEALR  C PSEL+Y++S
Sbjct: 1512 SRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICLPSELEYIRS 1571

Query: 1569 LSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFATAYFKYLSEAIRTGCPT 1628
            LSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+FA AYFKYLSE+I T  PT
Sbjct: 1572 LSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLSESISTKSPT 1631

Query: 1629 CLAKVVGIYQVSSKHLKGGKESKTDVLVMENLLFGHNVTRLYDLKGSCRSRYNSDTSGKN 1688
            CLAK++GIYQV++K LK GKE+K DVL+MENLLFG  V RLYDLKGS R+RYN D+SG N
Sbjct: 1632 CLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRARYNPDSSGSN 1691

Query: 1689 KVLLDQNLIESMPTSPIFVASKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELV 1748
            KVLLDQNLIE+MPTSPIFV +KAKRLLERAVWNDT+FLA  DVMDYSLLVG+DEEK ELV
Sbjct: 1692 KVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVGVDEEKNELV 1751

Query: 1749 LGIIDFMRQYTWDKHLETWVKNSGILGGPKNASPTVISPHQYKKRFRKAMTTYFLMVPDQ 1767
            LGIIDF+RQYTWDKHLE+WVK +GILGGPKN +PTVISP QYK+RFRKAMTTYFLMVPDQ
Sbjct: 1752 LGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAPTVISPKQYKRRFRKAMTTYFLMVPDQ 1782

BLAST of Sgr019899 vs. TAIR 10
Match: AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 1651.0 bits (4274), Expect = 0.0e+00
Identity = 955/1831 (52.16%), Postives = 1203/1831 (65.70%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            M   ++K    V +VKSW+P K + +N+SRDFWMPD+SC VCY+CD+QFT  NRRHHCR 
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFC+KC AN IP+PS ++  + E+ E+IRVCNYC+KQW +      N  + +S   S
Sbjct: 61   CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAIT-------DEQDNLLN 180
             SPS+ S++ST    T +S+   I ST   S     +P  S  ++        EQ N  +
Sbjct: 121  SSPSARSVAST----TSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQNAKS 180

Query: 181  GKCINRGTSLMTSSSSYYGYY----RSD---DEDDDYYYGIYRSDSESRHISQSDDYYGA 240
             +  +    ++ SS +   ++    RSD   D+DDD     Y+SD  ++  +Q +DYYGA
Sbjct: 181  RRSSDHYGHVLDSSDNQVEFFVNSGRSDGEADDDDD-----YQSDF-AQSYAQGNDYYGA 240

Query: 241  VNTD-IDHIAVQGLNLD-RENISTKGSSVIFDNQLPGADEESSEDSDENDKG----SECD 300
            +N D +DHI       D    I    S    D  L   + E+ + + + + G     E  
Sbjct: 241  INLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKEGS 300

Query: 301  FPTYCMQNKDNEPMDFQNNGLLWLPPEPEDEEDEK-ALLFDDDDDSCAPGEWGYLPSSFG 360
             P  C ++ + E +DF+++GLLWLPPEPE+EEDE+ A+L DDD D    G+WGYL  S  
Sbjct: 301  PP--CEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNS 360

Query: 361  SGEYPMNGRPNEEYKKTAKNVVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWE 420
              E   + +  ++     KNVVEGHFRALV QLLE +NLP+ +E +++GWLDIIT LSWE
Sbjct: 361  FNEKDFHSK--DKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWE 420

Query: 421  AAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRF 480
            AA +LKPDTSKSGGMDPGGYVKVKC+  G+RSES+VVKGV+CKKNVAHRRMT KI KPR 
Sbjct: 421  AATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRL 480

Query: 481  FILGGALEYQRVSNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLL 540
             ILGGALEYQR+SN LSSFDTLLQQE+DHLKMAVAKID+H+P++LLVEKSVSR AQEYLL
Sbjct: 481  LILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLL 540

Query: 541  SKNITLVLNIKRSLLERIACCTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQA 600
            +K+I+LVLNIKRSLLERI+ CTGA I+PS+D + S KLGYCD F VE FVE H     Q 
Sbjct: 541  AKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETH-VSPCQV 600

Query: 601  SKKLTKTLMFFEGCPKPLCCTILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEG 660
            +KK+ KTLMFF+GCPKPL CTILL+GA EDELK+VKHV+QY  FAAYHLALET FLADEG
Sbjct: 601  AKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEG 660

Query: 661  ASVPELPMKSPITVALPHNPSNIDRSISAIPGFT-------------GPPPVKESSIIGD 720
            AS+ ELP+++PITVALP  PS ++RSIS IPGFT             G P      + G+
Sbjct: 661  ASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGN 720

Query: 721  NVVKLDYSEPTLSQLKYNATSTSSSFLKHDVSTAY---HYMLSSK----FASEASRSLDI 780
                  + +    +L  N     S  L H++ T Y      ++SK      +  SR L  
Sbjct: 721  FTSSKTHFQ---GKLDGNDRIDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLSF 780

Query: 781  KECFMGMTTIKEEAGVDDDTISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRP 840
                       EE  V  D  S  SG  E ++  G   Y + +++ GN            
Sbjct: 781  H---------VEEPSVQKDQWSVLSGATEQVTDGG---YTNDSAVIGN------------ 840

Query: 841  ESTKANLVTKSSREDFPLSPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLG 900
            ++       +SS+ DF  S SDHQ+ILVSLSTRCVWKG++C+RAHLLRIKYYGSFDKPLG
Sbjct: 841  QNFNRQEQMESSKGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLG 900

Query: 901  RFLRDHLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHR 960
            RFLRD+LFDQ+  C SC MP+EAH+HCYTH QGSLTIS KKLP+  LPG+ EGKIWMWHR
Sbjct: 901  RFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPE-LLPGQREGKIWMWHR 960

Query: 961  CLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFY 1020
            CLKCPR NGFPPATRR++MSDAAWGLSFGKFLELSFSNHAAASRVA CGH LHRDCLRFY
Sbjct: 961  CLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFY 1020

Query: 1021 GFGRTVACFHYAAINVQSVHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALPQ 1080
            GFGR VACF YA+IN+ +V +PP+KL F++ N++W+ KE+ EV  +A+ LF EV  AL Q
Sbjct: 1021 GFGRMVACFRYASINIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQ 1080

Query: 1081 IPKNESGSEQEDLGVNELKHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEI 1140
            I     G+  +    N++K  ++EL  +    K++++   +++L+     GQP +DIL I
Sbjct: 1081 ISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQMLN-VVKDGQPTIDILLI 1140

Query: 1141 NKLHMHVLFLSYIWVQRLSFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQ 1200
            NKL   ++F SY W + L+ AA +  +N L++  N  P +  R + S+E+L +  + S  
Sbjct: 1141 NKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSIP 1200

Query: 1201 DEFFSGCDNDSLPSDSKHEIDLGKG----GNSGQINQSNEMEREKNIDL--------GGQ 1260
                    NDSL  D+ +E  L +G      SG+     ++  ++  D         GG+
Sbjct: 1201 THV--AICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDPSEGGK 1260

Query: 1261 EDELCLSPVNSEPLDVGITIGKAMSMGEYPIMDNLSGTLDAVWKAE--THPNSVTSEES- 1320
            ++ +  S V          +  A +  ++   D LS TLDA W  E  T  N +    S 
Sbjct: 1261 DNFVESSQV----------VKPAHTESQFQATD-LSDTLDAAWIGEQTTSENGIFRPPSR 1320

Query: 1321 GSQTNSAILEPLAGHSDFEKCIANLVGVEAAQLLHSALVLRRVDTVETCTSSMSM-SSSV 1380
             + TN   +  L            L+G E      S L  +   T +  T+ + + S S 
Sbjct: 1321 AASTNGTQIPDL-----------RLLGSE------SELNFKGGPTNDEHTTQVQLPSPSF 1380

Query: 1381 YNS--KISLLNVPKPEIADYDPAFISSFRELEKQGHLRLLMRVGANDTVIPVYDDEPTSI 1440
            Y S  K   LN  K  +A+  P ++SS+RELE +   RLL+ +G ND V+PVYDDEPTSI
Sbjct: 1381 YYSLNKNYSLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSI 1440

Query: 1441 IAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSANLNSLNSFKDAVSNAYKSHSSNEE 1500
            IAY+L   EY AQMS  ++  +  D+  S    DS NL SLNS  D   +  +S SS +E
Sbjct: 1441 IAYALTSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADE 1500

Query: 1501 STLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLGQVKYTITCYFAKRFEALRKKCCPS 1560
                +  S         LY KDLH R+SF +E P G+VKY++TCY+AK FEALR  CCPS
Sbjct: 1501 QVSQLLHSS--------LYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPS 1560

Query: 1561 ELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFATAYFKYLSEA 1620
            E D+++SL RC++WGAQGGKSNVFFAK+LD+RFIIKQVTK ELESFI+F  AYFKYL+E+
Sbjct: 1561 ETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTES 1620

Query: 1621 IRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVMENLLFGHNVTRLYDLKGSCRSRYN 1680
            I T  PT LAK++GIYQVSSKHLKGGKE K DVLVMENLLF  N TRLYDLKGS R+RYN
Sbjct: 1621 ISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYN 1680

Query: 1681 SDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLERAVWNDTSFLASIDVMDYSLLVGLD 1740
             DTSG N VLLDQNL+E+MPTSPIFV SKAKRLLERAVWNDTSFLASI VMDYSLLVG+D
Sbjct: 1681 PDTSGSNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVD 1740

Query: 1741 EEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGPKNASPTVISPHQYKKRFRKAMTTY 1773
            EE+ ELVLGIIDFMRQYTWDKHLETWVK SG+LGGPKN++PTVISP QYKKRFRKAMT Y
Sbjct: 1741 EERNELVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTAY 1748

BLAST of Sgr019899 vs. TAIR 10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 1650.6 bits (4273), Expect = 0.0e+00
Identity = 955/1832 (52.13%), Postives = 1203/1832 (65.67%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            M   ++K    V +VKSW+P K + +N+SRDFWMPD+SC VCY+CD+QFT  NRRHHCR 
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFC+KC AN IP+PS ++  + E+ E+IRVCNYC+KQW +      N  + +S   S
Sbjct: 61   CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAIT-------DEQDNLLN 180
             SPS+ S++ST    T +S+   I ST   S     +P  S  ++        EQ N  +
Sbjct: 121  SSPSARSVAST----TSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQNAKS 180

Query: 181  GKCINRGTSLMTSSSSYYGYY-----RSD---DEDDDYYYGIYRSDSESRHISQSDDYYG 240
             +  +    ++ SS +   ++     RSD   D+DDD     Y+SD  ++  +Q +DYYG
Sbjct: 181  RRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDD-----YQSDF-AQSYAQGNDYYG 240

Query: 241  AVNTD-IDHIAVQGLNLD-RENISTKGSSVIFDNQLPGADEESSEDSDENDKG----SEC 300
            A+N D +DHI       D    I    S    D  L   + E+ + + + + G     E 
Sbjct: 241  AINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKEG 300

Query: 301  DFPTYCMQNKDNEPMDFQNNGLLWLPPEPEDEEDEK-ALLFDDDDDSCAPGEWGYLPSSF 360
              P  C ++ + E +DF+++GLLWLPPEPE+EEDE+ A+L DDD D    G+WGYL  S 
Sbjct: 301  SPP--CEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSN 360

Query: 361  GSGEYPMNGRPNEEYKKTAKNVVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSW 420
               E   + +  ++     KNVVEGHFRALV QLLE +NLP+ +E +++GWLDIIT LSW
Sbjct: 361  SFNEKDFHSK--DKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSW 420

Query: 421  EAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPR 480
            EAA +LKPDTSKSGGMDPGGYVKVKC+  G+RSES+VVKGV+CKKNVAHRRMT KI KPR
Sbjct: 421  EAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPR 480

Query: 481  FFILGGALEYQRVSNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYL 540
              ILGGALEYQR+SN LSSFDTLLQQE+DHLKMAVAKID+H+P++LLVEKSVSR AQEYL
Sbjct: 481  LLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYL 540

Query: 541  LSKNITLVLNIKRSLLERIACCTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQ 600
            L+K+I+LVLNIKRSLLERI+ CTGA I+PS+D + S KLGYCD F VE FVE H     Q
Sbjct: 541  LAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETH-VSPCQ 600

Query: 601  ASKKLTKTLMFFEGCPKPLCCTILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADE 660
             +KK+ KTLMFF+GCPKPL CTILL+GA EDELK+VKHV+QY  FAAYHLALET FLADE
Sbjct: 601  VAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADE 660

Query: 661  GASVPELPMKSPITVALPHNPSNIDRSISAIPGFT-------------GPPPVKESSIIG 720
            GAS+ ELP+++PITVALP  PS ++RSIS IPGFT             G P      + G
Sbjct: 661  GASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTG 720

Query: 721  DNVVKLDYSEPTLSQLKYNATSTSSSFLKHDVSTAY---HYMLSSK----FASEASRSLD 780
            +      + +    +L  N     S  L H++ T Y      ++SK      +  SR L 
Sbjct: 721  NFTSSKTHFQ---GKLDGNDRIDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLS 780

Query: 781  IKECFMGMTTIKEEAGVDDDTISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLR 840
                        EE  V  D  S  SG  E ++  G   Y + +++ GN           
Sbjct: 781  FH---------VEEPSVQKDQWSVLSGATEQVTDGG---YTNDSAVIGN----------- 840

Query: 841  PESTKANLVTKSSREDFPLSPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPL 900
             ++       +SS+ DF  S SDHQ+ILVSLSTRCVWKG++C+RAHLLRIKYYGSFDKPL
Sbjct: 841  -QNFNRQEQMESSKGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPL 900

Query: 901  GRFLRDHLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWH 960
            GRFLRD+LFDQ+  C SC MP+EAH+HCYTH QGSLTIS KKLP+  LPG+ EGKIWMWH
Sbjct: 901  GRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPE-LLPGQREGKIWMWH 960

Query: 961  RCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRF 1020
            RCLKCPR NGFPPATRR++MSDAAWGLSFGKFLELSFSNHAAASRVA CGH LHRDCLRF
Sbjct: 961  RCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRF 1020

Query: 1021 YGFGRTVACFHYAAINVQSVHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALP 1080
            YGFGR VACF YA+IN+ +V +PP+KL F++ N++W+ KE+ EV  +A+ LF EV  AL 
Sbjct: 1021 YGFGRMVACFRYASINIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALS 1080

Query: 1081 QIPKNESGSEQEDLGVNELKHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILE 1140
            QI     G+  +    N++K  ++EL  +    K++++   +++L+     GQP +DIL 
Sbjct: 1081 QISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQMLN-VVKDGQPTIDILL 1140

Query: 1141 INKLHMHVLFLSYIWVQRLSFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASR 1200
            INKL   ++F SY W + L+ AA +  +N L++  N  P +  R + S+E+L +  + S 
Sbjct: 1141 INKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSI 1200

Query: 1201 QDEFFSGCDNDSLPSDSKHEIDLGKG----GNSGQINQSNEMEREKNIDL--------GG 1260
                     NDSL  D+ +E  L +G      SG+     ++  ++  D         GG
Sbjct: 1201 PTHV--AICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDPSEGG 1260

Query: 1261 QEDELCLSPVNSEPLDVGITIGKAMSMGEYPIMDNLSGTLDAVWKAE--THPNSVTSEES 1320
            +++ +  S V          +  A +  ++   D LS TLDA W  E  T  N +    S
Sbjct: 1261 KDNFVESSQV----------VKPAHTESQFQATD-LSDTLDAAWIGEQTTSENGIFRPPS 1320

Query: 1321 -GSQTNSAILEPLAGHSDFEKCIANLVGVEAAQLLHSALVLRRVDTVETCTSSMSM-SSS 1380
              + TN   +  L            L+G E      S L  +   T +  T+ + + S S
Sbjct: 1321 RAASTNGTQIPDL-----------RLLGSE------SELNFKGGPTNDEHTTQVQLPSPS 1380

Query: 1381 VYNS--KISLLNVPKPEIADYDPAFISSFRELEKQGHLRLLMRVGANDTVIPVYDDEPTS 1440
             Y S  K   LN  K  +A+  P ++SS+RELE +   RLL+ +G ND V+PVYDDEPTS
Sbjct: 1381 FYYSLNKNYSLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTS 1440

Query: 1441 IIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSANLNSLNSFKDAVSNAYKSHSSNE 1500
            IIAY+L   EY AQMS  ++  +  D+  S    DS NL SLNS  D   +  +S SS +
Sbjct: 1441 IIAYALTSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSAD 1500

Query: 1501 ESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLGQVKYTITCYFAKRFEALRKKCCP 1560
            E    +  S         LY KDLH R+SF +E P G+VKY++TCY+AK FEALR  CCP
Sbjct: 1501 EQVSQLLHSS--------LYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCP 1560

Query: 1561 SELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFATAYFKYLSE 1620
            SE D+++SL RC++WGAQGGKSNVFFAK+LD+RFIIKQVTK ELESFI+F  AYFKYL+E
Sbjct: 1561 SETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTE 1620

Query: 1621 AIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVMENLLFGHNVTRLYDLKGSCRSRY 1680
            +I T  PT LAK++GIYQVSSKHLKGGKE K DVLVMENLLF  N TRLYDLKGS R+RY
Sbjct: 1621 SISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARY 1680

Query: 1681 NSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLERAVWNDTSFLASIDVMDYSLLVGL 1740
            N DTSG N VLLDQNL+E+MPTSPIFV SKAKRLLERAVWNDTSFLASI VMDYSLLVG+
Sbjct: 1681 NPDTSGSNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGV 1740

Query: 1741 DEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGPKNASPTVISPHQYKKRFRKAMTT 1773
            DEE+ ELVLGIIDFMRQYTWDKHLETWVK SG+LGGPKN++PTVISP QYKKRFRKAMT 
Sbjct: 1741 DEERNELVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTA 1749

BLAST of Sgr019899 vs. TAIR 10
Match: AT4G33240.3 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 1649.0 bits (4269), Expect = 0.0e+00
Identity = 955/1832 (52.13%), Postives = 1199/1832 (65.45%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDPANVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            M   ++K    V +VKSW+P K + +N+SRDFWMPD+SC VCY+CD+QFT  NRRHHCR 
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAVSSAIS 120
            CGRVFC+KC AN IP+PS ++  + E+ E+IRVCNYC+KQW +      N  + +S   S
Sbjct: 61   CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121  QSPSSTSLSSTKSGYTCHSAISNIASTPCSSGPRYHDPYCSGAIT-------DEQDNLLN 180
             SPS+ S++ST    T +S+   I ST   S     +P  S  ++        EQ N  +
Sbjct: 121  SSPSARSVAST----TSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQNAKS 180

Query: 181  GKCINRGTSLMTSSSSYYGYY-----RSD---DEDDDYYYGIYRSDSESRHISQSDDYYG 240
             +  +    ++ SS +   ++     RSD   D+DDD     Y+SD  ++  +Q +DYYG
Sbjct: 181  RRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDD-----YQSDF-AQSYAQGNDYYG 240

Query: 241  AVNTD-IDHIAVQGLNLD-RENISTKGSSVIFDNQLPGADEESSEDSDENDKG----SEC 300
            A+N D +DHI       D    I    S    D  L   + E+ + + + + G     E 
Sbjct: 241  AINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKEG 300

Query: 301  DFPTYCMQNKDNEPMDFQNNGLLWLPPEPEDEEDEK-ALLFDDDDDSCAPGEWGYLPSSF 360
              P  C ++ + E +DF+++GLLWLPPEPE+EEDE+ A+L DDD D    G+WGYL  S 
Sbjct: 301  SPP--CEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSN 360

Query: 361  GSGEYPMNGRPNEEYKKTAKNVVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSW 420
               E   + +  ++     KNVVEGHFRALV QLLE +NLP+ +E +++GWLDIIT LSW
Sbjct: 361  SFNEKDFHSK--DKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSW 420

Query: 421  EAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPR 480
            EAA +LKPDTSKSGGMDPGGYVKVKC+  G+RSES+VVKGV+CKKNVAHRRMT KI KPR
Sbjct: 421  EAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPR 480

Query: 481  FFILGGALEYQRVSNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYL 540
              ILGGALEYQR+SN LSSFDTLLQQE+DHLKMAVAKID+H+P++LLVEKSVSR AQEYL
Sbjct: 481  LLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYL 540

Query: 541  LSKNITLVLNIKRSLLERIACCTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQ 600
            L+K+I+LVLNIKRSLLERI+ CTGA I+PS+D + S KLGYCD F VE FVE H     Q
Sbjct: 541  LAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETH-VSPCQ 600

Query: 601  ASKKLTKTLMFFEGCPKPLCCTILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADE 660
             +KK+ KTLMFF+GCPKPL CTILL+GA EDELK+VKHV+QY  FAAYHLALET FLADE
Sbjct: 601  VAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADE 660

Query: 661  GASVPELPMKSPITVALPHNPSNIDRSISAIPGFT-------------GPPPVKESSIIG 720
            GAS+ ELP+++PITVALP  PS ++RSIS IPGFT             G P      + G
Sbjct: 661  GASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTG 720

Query: 721  DNVVKLDYSEPTLSQLKYNATSTSSSFLKHDVSTAY---HYMLSSK----FASEASRSLD 780
            +      + +    +L  N     S  L H++ T Y      ++SK      +  SR L 
Sbjct: 721  NFTSSKTHFQ---GKLDGNDRIDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLS 780

Query: 781  IKECFMGMTTIKEEAGVDDDTISSCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLR 840
                        EE  V  D  S  SG  E ++  G   Y + +++ GN           
Sbjct: 781  FH---------VEEPSVQKDQWSVLSGATEQVTDGG---YTNDSAVIGN----------- 840

Query: 841  PESTKANLVTKSSREDFPLSPSDHQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPL 900
             ++       +SS+ DF  S SDHQ+ILVSLSTRCVWKG++C+RAHLLRIKYYGSFDKPL
Sbjct: 841  -QNFNRQEQMESSKGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPL 900

Query: 901  GRFLRDHLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWH 960
            GRFLRD+LFDQ+  C SC MP+EAH+HCYTH QGSLTIS KKLP+  LPG+ EGKIWMWH
Sbjct: 901  GRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPE-LLPGQREGKIWMWH 960

Query: 961  RCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRF 1020
            RCLKCPR NGFPPATRR++MSDAAWGLSFGKFLELSFSNHAAASRVA CGH LHRDCLRF
Sbjct: 961  RCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRF 1020

Query: 1021 YGFGRTVACFHYAAINVQSVHIPPSKLEFHHNNRDWIYKEANEVHNRAKSLFAEVCNALP 1080
            YGFGR VACF YA+IN+ +V +PP+KL F++ N++W+ KE+ EV  +A+ LF EV  AL 
Sbjct: 1021 YGFGRMVACFRYASINIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALS 1080

Query: 1081 QIPKNESGSEQEDLGVNELKHYIQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILE 1140
            QI     G+  +    N++K  ++EL  +    K++++     V       GQP +DIL 
Sbjct: 1081 QISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKQMLNVVKD-----GQPTIDILL 1140

Query: 1141 INKLHMHVLFLSYIWVQRLSFAATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASR 1200
            INKL   ++F SY W + L+ AA +  +N L++  N  P +  R + S+E+L +  + S 
Sbjct: 1141 INKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSI 1200

Query: 1201 QDEFFSGCDNDSLPSDSKHEIDLGKG----GNSGQINQSNEMEREKNIDL--------GG 1260
                     NDSL  D+ +E  L +G      SG+     ++  ++  D         GG
Sbjct: 1201 PTHV--AICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDPSEGG 1260

Query: 1261 QEDELCLSPVNSEPLDVGITIGKAMSMGEYPIMDNLSGTLDAVWKAE--THPNSVTSEES 1320
            +++ +  S V          +  A +  ++   D LS TLDA W  E  T  N +    S
Sbjct: 1261 KDNFVESSQV----------VKPAHTESQFQATD-LSDTLDAAWIGEQTTSENGIFRPPS 1320

Query: 1321 -GSQTNSAILEPLAGHSDFEKCIANLVGVEAAQLLHSALVLRRVDTVETCTSSMSM-SSS 1380
              + TN   +  L            L+G E      S L  +   T +  T+ + + S S
Sbjct: 1321 RAASTNGTQIPDL-----------RLLGSE------SELNFKGGPTNDEHTTQVQLPSPS 1380

Query: 1381 VYNS--KISLLNVPKPEIADYDPAFISSFRELEKQGHLRLLMRVGANDTVIPVYDDEPTS 1440
             Y S  K   LN  K  +A+  P ++SS+RELE +   RLL+ +G ND V+PVYDDEPTS
Sbjct: 1381 FYYSLNKNYSLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTS 1440

Query: 1441 IIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPPHDSANLNSLNSFKDAVSNAYKSHSSNE 1500
            IIAY+L   EY AQMS  ++  +  D+  S    DS NL SLNS  D   +  +S SS +
Sbjct: 1441 IIAYALTSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSAD 1500

Query: 1501 ESTLSMARSQSIQFEELLLYTKDLHTRVSFLNESPLGQVKYTITCYFAKRFEALRKKCCP 1560
            E    +  S         LY KDLH R+SF +E P G+VKY++TCY+AK FEALR  CCP
Sbjct: 1501 EQVSQLLHSS--------LYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCP 1560

Query: 1561 SELDYVKSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFATAYFKYLSE 1620
            SE D+++SL RC++WGAQGGKSNVFFAK+LD+RFIIKQVTK ELESFI+F  AYFKYL+E
Sbjct: 1561 SETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTE 1620

Query: 1621 AIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTDVLVMENLLFGHNVTRLYDLKGSCRSRY 1680
            +I T  PT LAK++GIYQVSSKHLKGGKE K DVLVMENLLF  N TRLYDLKGS R+RY
Sbjct: 1621 SISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARY 1680

Query: 1681 NSDTSGKNKVLLDQNLIESMPTSPIFVASKAKRLLERAVWNDTSFLASIDVMDYSLLVGL 1740
            N DTSG N VLLDQNL+E+MPTSPIFV SKAKRLLERAVWNDTSFLASI VMDYSLLVG+
Sbjct: 1681 NPDTSGSNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGV 1740

Query: 1741 DEEKQELVLGIIDFMRQYTWDKHLETWVKNSGILGGPKNASPTVISPHQYKKRFRKAMTT 1773
            DEE+ ELVLGIIDFMRQYTWDKHLETWVK SG+LGGPKN++PTVISP QYKKRFRKAMT 
Sbjct: 1741 DEERNELVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTA 1745

BLAST of Sgr019899 vs. TAIR 10
Match: AT1G71010.1 (FORMS APLOID AND BINUCLEATE CELLS 1C )

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 695/1778 (39.09%), Postives = 990/1778 (55.68%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWKPDP-----ANVSRDFWMPDKSCRVCYDCDSQFTFINRR 60
            MG P+  L DL+  V+SW+           ++  +DF +     ++C+DC    T + + 
Sbjct: 1    MGIPDGSLLDLIDKVRSWITSDSSDSLFLLSSSKQDFGIMPIVSKMCHDCG---TKVEQG 60

Query: 61   HHCRRCGRVFCSKCTANFIPAPSIDSTTAREDSEKIRVCNYCFKQWGKEPSSVQNDDNAV 120
            + C  CG  +C  C           S T     +  R C+   ++   +     +  ++ 
Sbjct: 61   YCCLSCGSCWCKSC-----------SDTEESKMKLCRECDAEVRELRVKSYDKVHPRDSP 120

Query: 121  SSAISQSPSSTSLSSTKSGYTCHSAISNIASTPC--SSGPRYHDPYCSGAITDEQDNLLN 180
                S +  S SL+S+     C     N+AS  C  S G      YC             
Sbjct: 121  DPPSSLATESESLASSLEIRDCR----NMASIRCYPSRGEEEEARYC------------- 180

Query: 181  GKCINRGTSLMTSSSSYYGYYRSDDEDDDY-----YYGIYRSDSESRHISQSDDYYGAVN 240
                  G  L++ SS  Y    SD E          +    S   S H S   + +  + 
Sbjct: 181  ------GKQLLSPSSDNY-QDSSDIESGSVSARHELFSCKSSAGSSPHDSPLRNNFSPLG 240

Query: 241  TDIDHIAVQGLNLDRENISTKGSSVIFDNQL-PGADEESSEDSDENDKGSECDFPTYCMQ 300
              + H A    +    +       ++ DN + PG      ED +E              +
Sbjct: 241  RFVQH-AKDLRSPTVCSFDNHQEQLLADNLVKPGQGVLEQEDHEEE-------------E 300

Query: 301  NKDNEPMDFQNNGLLWLPPEPEDEEDE---KALLFDDDDDSCAPGEWGYLPSSFGSGEYP 360
            +K  +P+DF+NNG +W PP PEDE D+       +DD+DD        +  SS  S   P
Sbjct: 301  DKLQQPLDFENNGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHIP 360

Query: 361  MNGRPNEEYKKTAKNVVEGHFRALVGQLLEAENLPIGDESNKDGWLDIITYLSWEAAAVL 420
               +  E   +  + VV  HFRALV +LL  E L   D+ +   WLDI+T L+W+AA  +
Sbjct: 361  TKEKLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFV 420

Query: 421  KPDTSKSGGMDPGGYVKVKCVAGGKRSESIVVKGVICKKNVAHRRMTRKISKPRFFILGG 480
            KPDT   G MDPG YVK+KCVA G ++ESI+++G++C KN+ H+RM  +   PR  +L G
Sbjct: 421  KPDTRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAG 480

Query: 481  ALEYQRVSNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNIT 540
            +LEYQRV+  L+SF+TLLQQE +H+K  +AKI++  PNVLLVEKS S +AQ+YLL K I+
Sbjct: 481  SLEYQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEIS 540

Query: 541  LVLNIKRSLLERIACCTGANIIPSVDHVASLKLGYCDAFRVETFVEEHDGGDGQASKKLT 600
            LVLN+KRSLL+RIA CTGA + PS+D +++ +LG+C+ FR E  +E+H+ G+ Q+++K +
Sbjct: 541  LVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGN-QSNRKPS 600

Query: 601  KTLMFFEGCPKPLCCTILLRGASEDELKQVKHVVQYATFAAYHLALETCFLADEGASVPE 660
            +TLM+FEGCP+ L CT++LRG+  +ELK+VKHV+QYA FAAYHL+LET FLADEGAS+P+
Sbjct: 601  RTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPK 660

Query: 661  LPMKSPITVALPHNPSNIDRSISAIPGFTGPPPVKESSIIGDNVVKLDYSEPTLSQLKYN 720
            + +K P  V        ID  IS I   T  P   +S  + +     D  E T    ++ 
Sbjct: 661  IRLKQPGMVRTASQRRIIDEGISLI---TQSPTETDSQALLETAAHED--EHTAPMPEHE 720

Query: 721  ATSTSSSFLKHDVSTAYHYMLSSKFASEASRSLD---IKECFMGMTTIKEEAGVDDDTIS 780
               +    L  D      +  SS+  +E S +L+            +  +   + + T+ 
Sbjct: 721  VCES----LCEDFDPTQIFPPSSEVETEQSDTLNGDFANNLVTRSYSSNQLNDLHEPTLC 780

Query: 781  SCSGIGEALSQCGNSCYISSNSLDGNTHSFIDLATLRPESTKANLVTKSSREDFPLSPSD 840
              S I E  +Q       S    +G       L   +      +        ++  +   
Sbjct: 781  LSSEIPETPTQ-----QPSGEEDNGRGEEENQLVNPQDLPQHESFYEDDVSSEYFSAADS 840

Query: 841  HQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDHLFDQNYRCHSCGMPSE 900
            HQ+ILVS S+RCV K ++C+R+ LLRIK+YGSFDKPLGR+L+D LFD+   C SC    +
Sbjct: 841  HQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELVD 900

Query: 901  AHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDA 960
            AHV CY+H  G+LTI+ ++LP   LPGE +GKIWMWHRCL+C   +G PPATRRV+MSDA
Sbjct: 901  AHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSDA 960

Query: 961  AWGLSFGKFLELSFSNHAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIP 1020
            AWGLSFGKFLELSFSNHA A+RVA CGH L RDCLRFYGFG  VA F Y+ IN+ +V +P
Sbjct: 961  AWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLLP 1020

Query: 1021 PSKLEFH-HNNRDWIYKEANEVHNRAKSLFAEVCNALPQIPKNESGSEQEDLGVNELKHY 1080
            PS LEF+ H  ++WI  EA E+  + ++++ E+ + L ++ +  S  E E     +L   
Sbjct: 1021 PSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQSEACDLHSR 1080

Query: 1081 IQELEQICNMEKEDFEVHFKEVLSGETIVGQPAVDILEINKLHMHVLFLSYIWVQRLSFA 1140
            I  L      EK++++   + +   E +  Q ++DILE+N+L   ++  ++ W       
Sbjct: 1081 IIGLIDQLVKEKDEYDDALQPIFE-ENLQIQGSLDILELNRLRRALMIGAHAW------- 1140

Query: 1141 ATLRNHNSLDSLSNDIPTLEERRLDSVEELVELNLASRQDEFFSGCDNDSLPSDSKHE-- 1200
                +H                      +L  LN   ++   F   D+++  +   H+  
Sbjct: 1141 ----DH----------------------QLYLLNSQLKKASVFKTGDDNAPRNPEMHDPP 1200

Query: 1201 -IDLGKGGNSGQINQSNEMEREKNIDLGGQEDELCLSPVNSEPLDVGITIGKAMSMGEYP 1260
             ID      S + ++ +  + E N D    E+        SE +D    +G   ++ +  
Sbjct: 1201 KIDRRMQEGSDERDEQSHTDSEANGDNKDPENIPSPGTSLSERID-SAWLGSFQNLEKAE 1260

Query: 1261 IMDNLSG--TLDAVWKAETHPNSVTSEESGSQTNSAILEPLAGHSDFEKCIANLVGVEAA 1320
             +    G   +++  +    P  V S +S  +    I + L   S +         +   
Sbjct: 1261 TIAETEGFSAVNSSLRRLARPIRVQSFDSAIRFQERIQKGLPPSSLY---------LSTL 1320

Query: 1321 QLLHSALVLRRV--DTVETCTSSMSMSSSVYNSKISLLNVPKPEIADYDPAFISSFRELE 1380
            +  H++   R +  D V     + S    +   K+ L       I    P +ISS  ++ 
Sbjct: 1321 RSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQKLDL-------IVGSAPTYISSASQM- 1380

Query: 1381 KQGHLRLLMRVGANDTVIPVYDDEPTSIIAYSLVMPEYHAQMSEPERVIEAGDTTRSSPP 1440
              G   L+ + G ND V+PVYDD+P S+++Y++   EY       E ++  G  + SS  
Sbjct: 1381 ADGARMLIPQRGLNDIVVPVYDDDPASVVSYAINSKEY------KEWIVNKGLASSSS-- 1440

Query: 1441 HDSANLNSLNSFKDAVSNAYKSHSSNEESTLSMARSQSIQFEELLLYTKDLHTRVSFLNE 1500
              S+NLN+  S   A S  ++S S + +         S    +    T     R S  + 
Sbjct: 1441 --SSNLNNRESEPSAFS-TWRSLSMDVDYIQHAVYGSSQDDRKSPHLTISFSDRASSSST 1500

Query: 1501 SPLGQVKYTITCYFAKRFEALRKKCCPSELDYVKSLSRCKRWGAQGGKSNVFFAKTLDER 1560
            +  G+VK+++TCYFA +F+ LRK CCPSE+D+V+SLSRC+RW AQGGKSNV+FAK+LDER
Sbjct: 1501 ATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFAKSLDER 1560

Query: 1561 FIIKQVTKIELESFIQFATAYFKYLSEAIRTGCPTCLAKVVGIYQVSSKHLKGGKESKTD 1620
            FIIKQV K EL+SF  FA  YFKYL E++ +G PTCLAK++GIYQVS KH KGGKE+K D
Sbjct: 1561 FIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGGKETKMD 1620

Query: 1621 VLVMENLLFGHNVTRLYDLKGSCRSRYNSDTSGKNKVLLDQNLIESMPTSPIFVASKAKR 1680
            ++VMENL +   ++R+YDLKGS RSRYN +TSG +KVLLD NL+E++ T PIF+ SKAKR
Sbjct: 1621 LMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLETLRTEPIFLGSKAKR 1648

Query: 1681 LLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVLGIIDFMRQYTWDKHLETWVKNSGI 1740
             LERA+WNDT+FLAS+DVMDYSLLVG DEE++ELVLGIIDFMRQYTWDKHLETWVK SGI
Sbjct: 1681 SLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGIIDFMRQYTWDKHLETWVKASGI 1648

Query: 1741 LGGPKNASPTVISPHQYKKRFRKAMTTYFLMVPDQWPS 1752
            LGGPKNASPT++SP QYK+RFRKAMTTYFL VP+ W S
Sbjct: 1741 LGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEPWTS 1648