Sgr019857 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr019857
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	rRNA N-glycosidase
Location	tig00153419: 1115864 .. 1117431 (+)
RNA-Seq Expression	Sgr019857
Synteny	Sgr019857

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAGTTTTAGTGCTTTGCATTGTTGTTGCCCTTTCTCTTGCCACTCATGGCATTGAAGCTAACCTGTGTTTATCACAGGCAGAATTTCCCGCTGATACTTACAAAAGCTTTATCCTCAATCTGCGTCAACAACTTAGCTTTGAAGCCAATCATCTCTACGGTATACCTGTCTTGGATCACTCTGTGTCAAATTCTCGTCGTTTCCATTCCACACATCTCACAAACGCAAACGATGAAACCATCATTTTGGCCATAGACGTAGAGGATGCTGGAGTTGTGGCATATCAAGCAGGCAGCGAATCATATTTTTTTCCGAATGCTCCACAAGTAGCTTTTCATATTATCTTCTCTAACACCAATCGAAACCTTCTGAATTTCAACAACACCTTTCAATCTATTGAGAAAGCAGCTAATACTACGAGGCTGGAAACTCTCCTTGGAGTGGATCCTTTAGAGTCAGCCATCTCCAACTTGTTTCAATATCGTCGTGAGTTTGTTCCTGTATCACTTCTTGTAGTCATCCAAATGGTCATAGAAGCTTCCAAGTACAAATTCATTGAGCAAAGTATTACTAATAGCATAAGGGATAGATATAATTTCACACCCGGCCTTGCAATCGTGAGCTTAGAGAACAATTGGTCAGAACTTTCTTTACAAATTCAAGCATCAATGTCATTACAAGGACTATTTGGAAGTGTCATCGAGCTTTATAATTCCAACAATGAACTTATTGGAGTGGATAGCATATACTATCCAATCATAGTTGCCAACATTGCTTTGCAACAATATCATTGTCAAGTTAACATTAGGGATGGCATTGTGGTTATGCGCAACTACATAAAGATGCCTGGCATTGGAGACAATGCCCGAAGCATGATCCATGCTATGTAGAAGCACGCACCACACGCATTAGTGGTCGAGAGGGATTTTGTGCAGATGTAGCAGGTGAGTTGTCTGCTGACGGTTCTCGCCTTATTTTGTACCCGTGTGGACAACAAGCGAATCAAAAATGGACATTTCATAGTGATCAGACAATTCGCTCCTTGGGCAAATGCTTGACCACTAATACCTCAAGCTTTGGGAACTATCTAGTGATTTACAATTGTGATAAGGCTACACAAAAAGACATTACTTGGGATGTTTCTATTAGCGGTACGATTATGAACCCAAGCTCTAACCTTGTCTTAACGGCAAATAAAGGCACAAGGAGCACCAACTTGACAGTGGAGGCCAACACATATGCTGCTAGTCAAGGTTGGAGAGTTGGGGATTATGTCGAGCCGATCATAACTTCCATTATAGGGCTTAAGGAGATGTGTTTGGAGGCAACAGAAAATAACACAAACATGTGGTTAGAAGAATGTGTGAAGAACAAGATGGAGCAATCTTGGGCACTTTACAGCGACGGAACTATTCGAGTTAACAATAATCGTAGCTTATGTGTGACCTCTTCTCTCAATAGTTCATCGAAGGTTATCACCATCCTTAGCTGCAATGGGTTAGTTAACCAACGTTGGCAATTCATAGCTGATGGCATAATCTTAAACCCCAAACTGAAATGGTGA

mRNA sequence

ATGAGAGTTTTAGTGCTTTGCATTGTTGTTGCCCTTTCTCTTGCCACTCATGGCATTGAAGCTAACCTGTGTTTATCACAGGCAGAATTTCCCGCTGATACTTACAAAAGCTTTATCCTCAATCTGCGTCAACAACTTAGCTTTGAAGCCAATCATCTCTACGGTATACCTGTCTTGGATCACTCTGTGTCAAATTCTCGTCGTTTCCATTCCACACATCTCACAAACGCAAACGATGAAACCATCATTTTGGCCATAGACGTAGAGGATGCTGGAGTTGTGGCATATCAAGCAGGCAGCGAATCATATTTTTTTCCGAATGCTCCACAAGTAGCTTTTCATATTATCTTCTCTAACACCAATCGAAACCTTCTGAATTTCAACAACACCTTTCAATCTATTGAGAAAGCAGCTAATACTACGAGGCTGGAAACTCTCCTTGGAGTGGATCCTTTAGAGTCAGCCATCTCCAACTTGTTTCAATATCGTCGTGAGTTTGTTCCTGTATCACTTCTTGTAGTCATCCAAATGGTCATAGAAGCTTCCAAGTACAAATTCATTGAGCAAAGTATTACTAATAGCATAAGGGATAGATATAATTTCACACCCGGCCTTGCAATCGTGAGCTTAGAGAACAATTGGTCAGAACTTTCTTTACAAATTCAAGCATCAATGTCATTACAAGGACTATTTGGAAATGCCTGGCATTGGAGACAATGCCCGAAGCATGATCCATGCTATGTAGAAGCACGCACCACACGCATTAGTGGTCGAGAGGGATTTTGTGCAGATGTAGCAGGTGAGTTGTCTGCTGACGGTTCTCGCCTTATTTTGTACCCGTGTGGACAACAAGCGAATCAAAAATGGACATTTCATAGTGATCAGACAATTCGCTCCTTGGGCAAATGCTTGACCACTAATACCTCAAGCTTTGGGAACTATCTAGTGATTTACAATTGTGATAAGGCTACACAAAAAGACATTACTTGGGATGTTTCTATTAGCGGTACGATTATGAACCCAAGCTCTAACCTTGTCTTAACGGCAAATAAAGGCACAAGGAGCACCAACTTGACAGTGGAGGCCAACACATATGCTGCTAGTCAAGGTTGGAGAGTTGGGGATTATGTCGAGCCGATCATAACTTCCATTATAGGGCTTAAGGAGATGTGTTTGGAGGCAACAGAAAATAACACAAACATGTGGTTAGAAGAATGTGTGAAGAACAAGATGGAGCAATCTTGGGCACTTTACAGCGACGGAACTATTCGAGTTAACAATAATCGTAGCTTATGTGTGACCTCTTCTCTCAATAGTTCATCGAAGGTTATCACCATCCTTAGCTGCAATGGGTTAGTTAACCAACGTTGGCAATTCATAGCTGATGGCATAATCTTAAACCCCAAACTGAAATGGTGA

Coding sequence (CDS)

ATGAGAGTTTTAGTGCTTTGCATTGTTGTTGCCCTTTCTCTTGCCACTCATGGCATTGAAGCTAACCTGTGTTTATCACAGGCAGAATTTCCCGCTGATACTTACAAAAGCTTTATCCTCAATCTGCGTCAACAACTTAGCTTTGAAGCCAATCATCTCTACGGTATACCTGTCTTGGATCACTCTGTGTCAAATTCTCGTCGTTTCCATTCCACACATCTCACAAACGCAAACGATGAAACCATCATTTTGGCCATAGACGTAGAGGATGCTGGAGTTGTGGCATATCAAGCAGGCAGCGAATCATATTTTTTTCCGAATGCTCCACAAGTAGCTTTTCATATTATCTTCTCTAACACCAATCGAAACCTTCTGAATTTCAACAACACCTTTCAATCTATTGAGAAAGCAGCTAATACTACGAGGCTGGAAACTCTCCTTGGAGTGGATCCTTTAGAGTCAGCCATCTCCAACTTGTTTCAATATCGTCGTGAGTTTGTTCCTGTATCACTTCTTGTAGTCATCCAAATGGTCATAGAAGCTTCCAAGTACAAATTCATTGAGCAAAGTATTACTAATAGCATAAGGGATAGATATAATTTCACACCCGGCCTTGCAATCGTGAGCTTAGAGAACAATTGGTCAGAACTTTCTTTACAAATTCAAGCATCAATGTCATTACAAGGACTATTTGGAAATGCCTGGCATTGGAGACAATGCCCGAAGCATGATCCATGCTATGTAGAAGCACGCACCACACGCATTAGTGGTCGAGAGGGATTTTGTGCAGATGTAGCAGGTGAGTTGTCTGCTGACGGTTCTCGCCTTATTTTGTACCCGTGTGGACAACAAGCGAATCAAAAATGGACATTTCATAGTGATCAGACAATTCGCTCCTTGGGCAAATGCTTGACCACTAATACCTCAAGCTTTGGGAACTATCTAGTGATTTACAATTGTGATAAGGCTACACAAAAAGACATTACTTGGGATGTTTCTATTAGCGGTACGATTATGAACCCAAGCTCTAACCTTGTCTTAACGGCAAATAAAGGCACAAGGAGCACCAACTTGACAGTGGAGGCCAACACATATGCTGCTAGTCAAGGTTGGAGAGTTGGGGATTATGTCGAGCCGATCATAACTTCCATTATAGGGCTTAAGGAGATGTGTTTGGAGGCAACAGAAAATAACACAAACATGTGGTTAGAAGAATGTGTGAAGAACAAGATGGAGCAATCTTGGGCACTTTACAGCGACGGAACTATTCGAGTTAACAATAATCGTAGCTTATGTGTGACCTCTTCTCTCAATAGTTCATCGAAGGTTATCACCATCCTTAGCTGCAATGGGTTAGTTAACCAACGTTGGCAATTCATAGCTGATGGCATAATCTTAAACCCCAAACTGAAATGGTGA

Protein sequence

MRVLVLCIVVALSLATHGIEANLCLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPVLDHSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGSESYFFPNAPQVAFHIIFSNTNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVIEASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFGNAWHWRQCPKHDPCYVEARTTRISGREGFCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIYNCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVEPIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLNSSSKVITILSCNGLVNQRWQFIADGIILNPKLKW

Homology

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BLAST of Sgr019857 vs. NCBI nr
Match: U3KRF8.2 (RecName: Full=Seed lectin; Short=SGSL; Contains: RecName: Full=Seed lectin Aalpha chain; Contains: RecName: Full=Seed lectin Abeta chain; Contains: RecName: Full=Seed lectin B chain [Trichosanthes anguina])

HSP 1 Score: 558.1 bits (1437), Expect = 7.1e-155
Identity = 293/483 (60.66%), Postives = 358/483 (74.12%), Query Frame = 0


Query: 20  EANLCLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPVLDHSVSNSRRFHSTHLTNAND 79
           +ANL LS+A   + TYK+FI  +R++L  E   LYGIPVL HS+SNS RF+   LT+  D
Sbjct: 3   QANLRLSEAN--SGTYKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQD 62

Query: 80  ETIILAIDVEDAGVVAYQ-AGS-ESYFFPNAPQVAFHIIFSNTNRNLLNFNNTFQSIEKA 139
           E+I LAIDVED   VAYQ AGS ESYFF NAPQ+AFH +F++T++N+LNF+NTF+S+E A
Sbjct: 63  ESITLAIDVEDMVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENA 122

Query: 140 ANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVIEASKYKFIEQSITNSIRD 199
           A TTR   +LGVDPL+ AISNLF    + +P+S LV+IQMV+EASK++FIEQS+  S ++
Sbjct: 123 AGTTRQTIVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKN 182

Query: 200 RYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFGNA----------------------- 259
              F P LAIVSLE+NWSE+SLQIQAS SLQGLFG+                        
Sbjct: 183 EKTFLPDLAIVSLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILA 242

Query: 260 ------WHWRQCPKHDPCYVEARTTRISGREGFCADVAGELSADGSRLILYPCGQQANQK 319
                 +H +     + C VE RTTRISGR+  C DVAG L++DGSRLILYPCGQQ NQK
Sbjct: 243 NVALQLYHCQVSTGDNECLVETRTTRISGRDALCVDVAGALTSDGSRLILYPCGQQVNQK 302

Query: 320 WTFHSDQTIRSLGKCLTTNTSSFGNYLVIYNCDKATQKDITWDVSISGTIMNPS-SNLVL 379
           WTFHSD T+RSLGKCL TN S FGN +VIY+C K   +DI+WDVS+ GTIMNP+  +L L
Sbjct: 303 WTFHSDGTVRSLGKCLATNNSKFGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLAL 362

Query: 380 TANKGTRSTNLTVEANTYAASQGWRVGDYVEPIITSIIGLKEMCLEATENNTNMWLEECV 439
           T+NK TRSTNLT+E NTY+ASQGWRVG+YV+PII SI+GL +MCLEAT+ NTNMWLEECV
Sbjct: 363 TSNKATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGNTNMWLEECV 422

Query: 440 KNKMEQSWALYSDGTIRVNNNRSLCVT--SSLNSSSKVITILSCNGLVNQRWQFIADGII 469
            N+ EQSWALYSDGTIRV++NR LCVT  SS   + KVITIL+C+G  NQRW F+ADG I
Sbjct: 423 PNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSNNQRWVFLADGSI 482

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BLAST of Sgr019857 vs. NCBI nr
Match: XP_022156703.1 (seed lectin-like [Momordica charantia] >BAH05018.1 galactose-binding type-2 ribosome-inactivating protein [Momordica charantia])

HSP 1 Score: 533.9 bits (1374), Expect = 1.4e-147
Identity = 285/510 (55.88%), Postives = 356/510 (69.80%), Query Frame = 0


Query: 1   MRVLVLCIVVALSLATHGIEANLCLSQAEFPADTYKSFILNLRQQLSFEANH-LYGIPVL 60
           MRVL + IVVALSL  +GIE NL LSQ+ F ADTYKSFI NLR+QL+  A++   GIP+L
Sbjct: 3   MRVLAVYIVVALSLTINGIECNLSLSQSNFSADTYKSFIKNLRKQLTIGASYGSAGIPIL 62

Query: 61  DHSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGSESYFFPNAPQVAFHIIFSN 120
            HSV    RF    LTN ++ETI LAI+VEDAG  AY+A   SYFF NAP +A ++IF++
Sbjct: 63  KHSVPICERFLLVDLTNGDNETITLAINVEDAGFAAYRAADRSYFFQNAPPIASYVIFTD 122

Query: 121 TNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVI 180
           TN+N++NFNNTF+SIE    TTR ET LG+   E++I +LF +   +VP S LV+IQMV+
Sbjct: 123 TNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVPTSFLVLIQMVL 182

Query: 181 EASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFGN------ 240
           EA+K+KFIEQ + +SI D  +FTPGLA++SLE NW++LSLQ+QAS SL G+FG+      
Sbjct: 183 EAAKFKFIEQKVIHSIMDMEDFTPGLAMLSLEENWTQLSLQLQASESLNGVFGDSVSLYN 242

Query: 241 --------------------AWHWRQCP---------------KHDPCYVEARTTRISGR 300
                               A+   QCP                ++ C  + RTTRISGR
Sbjct: 243 SMDEPIGVDSMYYPILTANMAFQLYQCPYGVIRMMPTITMPNQNNEQCSPQQRTTRISGR 302

Query: 301 EGFCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIY 360
           +G C DV G L+ADGSR+ILYPCGQQ NQ+WTF+ D TIRSLGKCL T+  S G+ +VI 
Sbjct: 303 DGLCVDVYGALTADGSRVILYPCGQQQNQQWTFYPDNTIRSLGKCLATSALSSGSNVVIT 362

Query: 361 NCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVE 420
           NCD     D  W VS SGT+MN SS+LVLTAN  T  TNLT E N +AA Q WR+G+YVE
Sbjct: 363 NCDYLRYDD-GWMVSSSGTMMNKSSHLVLTANAATSRTNLTGENNVFAAKQAWRIGNYVE 422

Query: 421 PIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLN 469
           PI+T+IIGL+ MCLEAT+N+TN+WLE CVKNK +Q WALYSD TIRVNNNR+LCV+SS +
Sbjct: 423 PIVTTIIGLRHMCLEATDNDTNVWLESCVKNKTKQYWALYSDDTIRVNNNRNLCVSSSTD 482

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BLAST of Sgr019857 vs. NCBI nr
Match: XP_038884532.1 (seed lectin-like [Benincasa hispida])

HSP 1 Score: 498.0 bits (1281), Expect = 8.7e-137
Identity = 274/509 (53.83%), Postives = 352/509 (69.16%), Query Frame = 0


Query: 1   MRVLVLCIVVALSLATHGIEANLCLSQAEFP-ADTYKSFILNLRQQLSFEAN-HLYGIPV 60
           MRVLV  +++AL +A  GI A      A+ P +D YK++I N+RQ+ + + +  LYGIP+
Sbjct: 15  MRVLV-ALIIALCIAIDGITA------AKTPISDRYKTYIQNVRQKFASQTHKQLYGIPM 74

Query: 61  LDHSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAG-SESYFFPNAPQVAFHIIF 120
           L HS+SNS RF      N   +TI LA+D +D  VVAY AG ++SYFF NAP   F I+F
Sbjct: 75  LQHSLSNSDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNAPLFTFDILF 134

Query: 121 SNTNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQM 180
            NT++ LL+++NTF+SIE AANTTR    LG++P  SAISNLF YR +   VS LVV QM
Sbjct: 135 PNTHQTLLSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLAAVSFLVVFQM 194

Query: 181 VIEASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFG----- 240
           + EA+K+KFIEQ+I N I++  +FTP LAIVSLE+NWS+LSLQIQ+S SLQGLFG     
Sbjct: 195 IFEAAKFKFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVML 254

Query: 241 -----------------------------NAWHWRQCP---KHDPCYVEARTTRISGREG 300
                                        N   + + P   ++  CYVE  TTRISGR+G
Sbjct: 255 HNSKNEAIEVDSIYFPIILNNLALQLYHCNTQDYIKLPSLVENSRCYVEEHTTRISGRDG 314

Query: 301 FCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIYNC 360
            CADV     ADGS +I +PC QQANQ+WTF+SD+TIRSLGKCL  + SS     VIY+C
Sbjct: 315 LCADV-----ADGSHVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKSSSNPLAVIYDC 374

Query: 361 DKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVEPI 420
            K +Q+D TW+VS+SGTIMN  ++ VLT+N G   + LT+E N Y  +QGWRVG++V+PI
Sbjct: 375 SKVSQQDATWEVSLSGTIMNTYNDSVLTSNNG---STLTMEDNAYDGNQGWRVGNFVDPI 434

Query: 421 ITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLNSS 470
           +TSIIG+KEMCLEATE NTN+WLEECVK+K+EQSW LYSDG+IRVNNN SLCVT+S++S 
Sbjct: 435 VTSIIGVKEMCLEATEGNTNIWLEECVKDKIEQSWTLYSDGSIRVNNNHSLCVTASIHSR 494

[Close]

BLAST of Sgr019857 vs. NCBI nr
Match: XP_004140357.1 (seed lectin [Cucumis sativus] >KGN51070.1 hypothetical protein Csa_008446 [Cucumis sativus])

HSP 1 Score: 487.6 bits (1254), Expect = 1.2e-133
Identity = 268/511 (52.45%), Postives = 336/511 (65.75%), Query Frame = 0


Query: 1   MRVLVLCIVVALSLATHGIEANLCLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPVLD 60
           MRVL L  +V LS+AT+ +     +       D Y+S+I  +RQ+   + + LYGIPVL 
Sbjct: 1   MRVL-LAFIVTLSIATNALSKRPVV-------DVYQSYIQTIRQKFGSKTHKLYGIPVLH 60

Query: 61  HSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAG-SESYFFPNAPQVAFHIIFSN 120
           HS+SNS RFH     N   +TI  A+D +D  VVAY AG ++SYFF NAP+ AF I+F  
Sbjct: 61  HSLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPK 120

Query: 121 TNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVI 180
           TN+NLLNF+N+F+SIE AANTTR  T LG+ P  +AI+NLF Y     PVS L+V QMV 
Sbjct: 121 TNQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVF 180

Query: 181 EASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFG------- 240
           E++K+KFIEQ I NSI +   FTP LA++SLE+NWSELSL+IQAS SLQGLFG       
Sbjct: 181 ESAKFKFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYN 240

Query: 241 ---------------------------NAWHWRQCP-------KHDPCYVEARTTRISGR 300
                                      N   + + P       ++  CYV+ RT RISG+
Sbjct: 241 SKNEAVEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQ 300

Query: 301 EGFCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIY 360
           +G CADVA     DGS +I  PCGQQANQ+WTFH D TIRS  KCL  N S      VI 
Sbjct: 301 DGLCADVA----RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQ 360

Query: 361 NCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVE 420
           NC+K +Q+D TWDVSISGTIMNP+ +LVLT+  G    +L+++ N Y  +QGWRVG+YVE
Sbjct: 361 NCNKVSQEDATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVE 420

Query: 421 PIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLN 470
           PII SIIG+K+MCLEATE NTN+WLEECVKNK+EQSWA++SDG+IRVNN+ SLC+T+S  
Sbjct: 421 PIIASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSI 480

[Close]

BLAST of Sgr019857 vs. NCBI nr
Match: XP_038884101.1 (seed lectin-like [Benincasa hispida])

HSP 1 Score: 484.2 bits (1245), Expect = 1.3e-132
Identity = 262/511 (51.27%), Postives = 335/511 (65.56%), Query Frame = 0


Query: 1   MRVLVLCIVVALSLATHGIEAN--LCLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPV 60
           M  L   I+V LSLAT  I+ N  L LS ++   D YK+FI  +R++L+   + LY IP+
Sbjct: 1   MTSLTFYIIVFLSLATRSIDGNQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPI 60

Query: 61  LDHSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGSESYFFPNAPQVAFHIIFS 120
           L +S+  +  F + +L NAN+ETI+LAIDV + GVV Y++ + SY F NAP  A  I+F 
Sbjct: 61  LKNSLPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFP 120

Query: 121 NTNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMV 180
           +T R LL F++ ++SIEKA+  +RL+TLLG DPL SAISNLF Y +E +P S LV++QMV
Sbjct: 121 STCRVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMV 180

Query: 181 IEASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFGNAWHW- 240
           +E  K+KFIEQS+  S++  YNF P LA++SL++NW++LSLQIQAS SLQGLFG A ++ 
Sbjct: 181 LEGVKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFY 240

Query: 241 -------------------------RQC------------PKHDPCYVEARTTRISGREG 300
                                     QC                PCY++ +T+ ISGREG
Sbjct: 241 DSNNRIIQVDSIYYPIIITNIALQLYQCNVSTNFIRMPSITSDSPCYIQTQTSSISGREG 300

Query: 301 FCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIYNC 360
           FC D    +  DG+ +I  PC  Q+N+KW+F  D TIR   KCLT +TS F   +V+YNC
Sbjct: 301 FCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRF---VVLYNC 360

Query: 361 DKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVEPI 420
            K   K   W+V+I GTI NPSS LVLTAN  T +T L VEAN Y   QGWRVG+YVEPI
Sbjct: 361 SKVEAKSTKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPI 420

Query: 421 ITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLNSS 472
           I SIIGL++MCLEAT NNTNMWLE CV NK EQ WA+YSDG+IRVN+ R+LCVTSS + S
Sbjct: 421 IGSIIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRS 480

[Close]

BLAST of Sgr019857 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 558.1 bits (1437), Expect = 9.3e-158
Identity = 293/483 (60.66%), Postives = 358/483 (74.12%), Query Frame = 0


Query: 20  EANLCLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPVLDHSVSNSRRFHSTHLTNAND 79
           +ANL LS+A   + TYK+FI  +R++L  E   LYGIPVL HS+SNS RF+   LT+  D
Sbjct: 3   QANLRLSEAN--SGTYKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQD 62

Query: 80  ETIILAIDVEDAGVVAYQ-AGS-ESYFFPNAPQVAFHIIFSNTNRNLLNFNNTFQSIEKA 139
           E+I LAIDVED   VAYQ AGS ESYFF NAPQ+AFH +F++T++N+LNF+NTF+S+E A
Sbjct: 63  ESITLAIDVEDMVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENA 122

Query: 140 ANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVIEASKYKFIEQSITNSIRD 199
           A TTR   +LGVDPL+ AISNLF    + +P+S LV+IQMV+EASK++FIEQS+  S ++
Sbjct: 123 AGTTRQTIVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKN 182

Query: 200 RYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFGNA----------------------- 259
              F P LAIVSLE+NWSE+SLQIQAS SLQGLFG+                        
Sbjct: 183 EKTFLPDLAIVSLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILA 242

Query: 260 ------WHWRQCPKHDPCYVEARTTRISGREGFCADVAGELSADGSRLILYPCGQQANQK 319
                 +H +     + C VE RTTRISGR+  C DVAG L++DGSRLILYPCGQQ NQK
Sbjct: 243 NVALQLYHCQVSTGDNECLVETRTTRISGRDALCVDVAGALTSDGSRLILYPCGQQVNQK 302

Query: 320 WTFHSDQTIRSLGKCLTTNTSSFGNYLVIYNCDKATQKDITWDVSISGTIMNPS-SNLVL 379
           WTFHSD T+RSLGKCL TN S FGN +VIY+C K   +DI+WDVS+ GTIMNP+  +L L
Sbjct: 303 WTFHSDGTVRSLGKCLATNNSKFGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLAL 362

Query: 380 TANKGTRSTNLTVEANTYAASQGWRVGDYVEPIITSIIGLKEMCLEATENNTNMWLEECV 439
           T+NK TRSTNLT+E NTY+ASQGWRVG+YV+PII SI+GL +MCLEAT+ NTNMWLEECV
Sbjct: 363 TSNKATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGNTNMWLEECV 422

Query: 440 KNKMEQSWALYSDGTIRVNNNRSLCVT--SSLNSSSKVITILSCNGLVNQRWQFIADGII 469
            N+ EQSWALYSDGTIRV++NR LCVT  SS   + KVITIL+C+G  NQRW F+ADG I
Sbjct: 423 PNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSNNQRWVFLADGSI 482

[Close]

BLAST of Sgr019857 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 350.1 bits (897), Expect = 3.8e-95
Identity = 206/518 (39.77%), Postives = 292/518 (56.37%), Query Frame = 0


Query: 3   VLVLCIVVALSLATHGIEANLCLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPVL--D 62
           VL +C V    +    +  NL  +++     TY+ F+ NLR+ ++     + G+PVL  +
Sbjct: 15  VLAICSVGIQGIDYPSVSFNLDGAKSA----TYRDFLSNLRKTVATGTYEVNGLPVLRRE 74

Query: 63  HSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGSESYFFPNAPQVAFHIIFSNT 122
             V    RF    LTN N  T+ LA+DV +  VVA+   + SYFF +A +V    +F  T
Sbjct: 75  SEVQVKSRFVLVPLTNYNGNTVTLAVDVTNLYVVAFSGNANSYFFKDATEVQKSNLFVGT 134

Query: 123 NRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVIE 182
            +N L+F   + ++E AANT R    LG  PL+ AI++L  Y  + V  SLLVVIQMV E
Sbjct: 135 KQNTLSFTGNYDNLETAANTRRESIELGPSPLDGAITSL--YHGDSVARSLLVVIQMVSE 194

Query: 183 ASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQI-QASMSLQGLFG-----NA 242
           A+++++IEQ +  S++   +FTP   ++S+ENNWS +SL+I QA  ++   FG     N 
Sbjct: 195 AARFRYIEQEVRRSLQQATSFTPNALMLSMENNWSSMSLEIQQAGNNVSPFFGTVQLLNY 254

Query: 243 WHWRQ----------------------CPKHD--------------------PCYVEART 302
            H  +                       P +D                     C +    
Sbjct: 255 DHTHRLVDNFEELYKITGIAILLFRCSSPSNDNAIRMPLDLAGEDNKYNDGETCTLRTSF 314

Query: 303 TR-ISGREGFCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSF 362
           TR I GR+G C DV      DG+ L L+PCG Q NQ+WTF SD TIRS+GKC+T N  + 
Sbjct: 315 TRNIVGRDGLCVDVRNGYDTDGTPLQLWPCGTQRNQRWTFDSDDTIRSMGKCMTANGLNN 374

Query: 363 GNYLVIYNCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGW 422
           G+ +VI+NC  A +  I W+V I G+I+NPSS LV+TA +    T L +E N YAASQGW
Sbjct: 375 GSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIYAASQGW 434

Query: 423 RVGDYVEPIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSL 470
            V + V+PI+ SI+G KEMCL++   N  +W+E+C    ++Q WALY D TIRVN+ R L
Sbjct: 435 TVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYGDRTIRVNSTRGL 494

[Close]

BLAST of Sgr019857 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.6e-85
Identity = 203/537 (37.80%), Postives = 283/537 (52.70%), Query Frame = 0


Query: 2   RVLVLCIVVALSLATHGIEANLCLSQAEFP--------ADTYKSFILNLRQQLSFEANHL 61
           ++L L ++    L  HG   +  ++   +P        ADTY+ F+  L++++    +  
Sbjct: 6   KLLYLAVLAICGLGIHGALTHPRVTPPVYPSVSFNLTGADTYEPFLRALQEKVILGNHTA 65

Query: 62  YGIPVL--DHSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGSESYFFPNAPQV 121
           + +PVL  +  VS+S RF    LTN + +T+ LAIDV +  VVA+ +  +SYFF  +  V
Sbjct: 66  FDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVNLYVVAFSSNGKSYFFSGSTAV 125

Query: 122 AFHIIFSNTNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQY---RREFVP 181
               +F +T +  LNF   + S+E+     R+   LG   L+ AIS+L  Y     +  P
Sbjct: 126 QRDNLFVDTTQEELNFTGNYTSLERQVGFGRVYIPLGPKSLDQAISSLRTYTLTAGDTKP 185

Query: 182 VS--LLVVIQMVIEASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMS 241
           ++  LLVVIQMV EA+++++IE  I  SI D   FTP L ++S+ENNWS +S +IQ +  
Sbjct: 186 LARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDLLMLSMENNWSSMSSEIQQAQP 245

Query: 242 LQGLFGNAWHWRQ--------------------------CPK------------------ 301
             G+F      R                           C                    
Sbjct: 246 -GGIFAGVVQLRDERNNSIEVTNFRRLFELTYIAVLLYGCAPVTSSSYSNNAIDAQIIKM 305

Query: 302 --------HDPCYVEARTTRISGREGFCADVAGELSADGSRLILYPCGQQANQKWTFHSD 361
                      C V   T RISG +G C DV      DG+ + L PCG + NQ WTF +D
Sbjct: 306 PVFRGGEYEKVCSVVEVTRRISGWDGLCVDVRYGHYIDGNPVQLRPCGNECNQLWTFRTD 365

Query: 362 QTIRSLGKCLTTNTSSFGNYLVIYNCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTR 421
            TIR LGKCLT ++S     ++IY+C+    +   W VSI GTI NP S LVLTA +   
Sbjct: 366 GTIRWLGKCLTASSS-----VMIYDCNTVPPEATKWVVSIDGTITNPHSGLVLTAPQAAE 425

Query: 422 STNLTVEANTYAASQGWRVGDYVEPIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQS 472
            T L++E N +AA QGW VGD VEP++T I+G K+MCL     N  +WLE+CV N+++Q 
Sbjct: 426 GTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLRENGENNFVWLEDCVLNRVQQE 485

[Close]

BLAST of Sgr019857 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 6.1e-85
Identity = 204/538 (37.92%), Postives = 283/538 (52.60%), Query Frame = 0


Query: 2   RVLVLCIVVALSLATHGIEANLCLSQAEFP--------ADTYKSFILNLRQQLSFEANHL 61
           ++L L ++    L  HG   +  ++   +P        ADTY  F+  L++++    +  
Sbjct: 6   KLLYLVVLAICGLGIHGALTHTRVTPPVYPSVSFNLTGADTYGPFLRALQEKVILGNHTA 65

Query: 62  YGIPVL--DHSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGSESYFFPNAPQV 121
           + +PVL  +  VS+S RF    LTN + +T+ LAIDV +  VVA+ +   SYFF  +  V
Sbjct: 66  FDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVNLYVVAFSSNGRSYFFSGSTAV 125

Query: 122 AFHIIFSNTNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQY---RREFVP 181
               +F +T +  LNF   + S+E+     R+   LG   L  AIS+L  Y     +  P
Sbjct: 126 QRDNLFVDTTQEELNFTGNYISLERQVGFGRVYIPLGPKSLAQAISSLRTYTLSAGDTKP 185

Query: 182 VS--LLVVIQMVIEASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMS 241
           ++  LLVVIQMV EA+++++IE  I  SI D   FTP L ++S+ENNWS +S +IQ +  
Sbjct: 186 LARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDLLMLSMENNWSSMSSEIQQAQP 245

Query: 242 LQGLFGNAWHWRQCPKHDP----------------------------------------- 301
             G+F      R   +++P                                         
Sbjct: 246 -GGIFPGVVQLRD-ERNNPIEVTNFRRLFELTYIAVLLYGCAPVTSNSYTNNAIDAQIIK 305

Query: 302 ------------CYVEARTTRISGREGFCADVAGELSADGSRLILYPCGQQANQKWTFHS 361
                       C V   T RISG +G C DV      DG+ + L PCG + NQ WTF +
Sbjct: 306 MPVFRGGGYEKVCSVVEVTRRISGWDGLCVDVRDGHYIDGNTVQLGPCGNECNQLWTFRT 365

Query: 362 DQTIRSLGKCLTTNTSSFGNYLVIYNCDKATQKDITWDVSISGTIMNPSSNLVLTANKGT 421
           D TIR LGKCLTT++S     ++IY+C+    +   W VS  GTI NP S LVLTA +  
Sbjct: 366 DGTIRWLGKCLTTSSS-----VMIYDCNTVPPEATKWVVSTDGTITNPRSGLVLTAPQAA 425

Query: 422 RSTNLTVEANTYAASQGWRVGDYVEPIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQ 472
             T L++E N +AA QGW VGD VEP++T I+G K+MCL     N  +WLE+CV N++EQ
Sbjct: 426 EGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLTENGENNFVWLEDCVLNRVEQ 485

[Close]

BLAST of Sgr019857 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.1e-81
Identity = 203/534 (38.01%), Postives = 284/534 (53.18%), Query Frame = 0


Query: 3   VLVLCIVVALSLATHG----------IEANLCLSQAEFPADTYKSFILNLRQQLSFEANH 62
           +L L ++    L  HG          +  ++  +  E  ++ Y+ F+  LR ++   ++ 
Sbjct: 7   ILYLVVLAICGLGIHGAHPTHSAPPTVYPSVSFNLTEANSNEYRHFLQELRGKVILGSHR 66

Query: 63  LYGIPVL--DHSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGSESYFFPNAPQ 122
            + +PVL  +  VS+S RF    LTN + + + LAIDV    VVA+     SYFF  + +
Sbjct: 67  AFDLPVLNPESKVSDSDRFVLVRLTNPSRKKVTLAIDVVTFYVVAFAQNDRSYFFSGSSE 126

Query: 123 VAFHIIFSNTNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYR-----RE 182
           V    +F +T +  LNF   + S+E      R+   LG   L  +IS+L  Y+      +
Sbjct: 127 VQRENLFVDTTQEDLNFKGDYTSLEHQVGFGRVYIPLGPKSLAQSISSLSTYKSSAGDNK 186

Query: 183 FVPVSLLVVIQMVIEASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASM 242
            +  SLLVVIQMV EA+++++I+  I  SI D   FTP L ++S+EN WS +S +IQ + 
Sbjct: 187 RLARSLLVVIQMVSEAARFRYIQLRIQASITDAKEFTPDLLMLSMENKWSSMSSEIQQAQ 246

Query: 243 -------------------------SLQGLFGNAWHWRQCP--KHDPCY----------- 302
                                     L  L   A     CP     P Y           
Sbjct: 247 PGGAFAQVVKLLDQRNHPIDVTNFRRLFQLTSVAVLLHGCPTVTKMPAYIIKMPVFNGGE 306

Query: 303 -------VEARTTRISGREGFCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSL 362
                  VE  T RI GR+GFCA+V      DG+ + L  CG+Q+NQ+WTF +D TI+SL
Sbjct: 307 DEERCSVVEEVTRRIGGRDGFCAEVKNGDEKDGTPVQLSSCGEQSNQQWTFSTDGTIQSL 366

Query: 363 GKCLTTNTSSFGNYLVIYNCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTV 422
           GKCLTT++S     ++IYNC     +   W VSI GTI NP S LVLTA K    T +++
Sbjct: 367 GKCLTTSSS-----VMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSL 426

Query: 423 EANTYAASQGWRVGDYVEPIITSIIGLKEMCLEATENNTNMWLEEC-VK--NKMEQSWAL 472
           E N +AA QGW VG+ VEP++T I+G ++MCLE    N ++ L +C VK  +K++Q WAL
Sbjct: 427 EKNVHAARQGWIVGN-VEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWAL 486

[Close]

BLAST of Sgr019857 vs. ExPASy TrEMBL
Match: B7X8M2 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MCL1 PE=1 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 6.9e-148
Identity = 285/510 (55.88%), Postives = 356/510 (69.80%), Query Frame = 0


Query: 1   MRVLVLCIVVALSLATHGIEANLCLSQAEFPADTYKSFILNLRQQLSFEANH-LYGIPVL 60
           MRVL + IVVALSL  +GIE NL LSQ+ F ADTYKSFI NLR+QL+  A++   GIP+L
Sbjct: 3   MRVLAVYIVVALSLTINGIECNLSLSQSNFSADTYKSFIKNLRKQLTIGASYGSAGIPIL 62

Query: 61  DHSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGSESYFFPNAPQVAFHIIFSN 120
            HSV    RF    LTN ++ETI LAI+VEDAG  AY+A   SYFF NAP +A ++IF++
Sbjct: 63  KHSVPICERFLLVDLTNGDNETITLAINVEDAGFAAYRAADRSYFFQNAPPIASYVIFTD 122

Query: 121 TNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVI 180
           TN+N++NFNNTF+SIE    TTR ET LG+   E++I +LF +   +VP S LV+IQMV+
Sbjct: 123 TNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVPTSFLVLIQMVL 182

Query: 181 EASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFGN------ 240
           EA+K+KFIEQ + +SI D  +FTPGLA++SLE NW++LSLQ+QAS SL G+FG+      
Sbjct: 183 EAAKFKFIEQKVIHSIMDMEDFTPGLAMLSLEENWTQLSLQLQASESLNGVFGDSVSLYN 242

Query: 241 --------------------AWHWRQCP---------------KHDPCYVEARTTRISGR 300
                               A+   QCP                ++ C  + RTTRISGR
Sbjct: 243 SMDEPIGVDSMYYPILTANMAFQLYQCPYGVIRMMPTITMPNQNNEQCSPQQRTTRISGR 302

Query: 301 EGFCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIY 360
           +G C DV G L+ADGSR+ILYPCGQQ NQ+WTF+ D TIRSLGKCL T+  S G+ +VI 
Sbjct: 303 DGLCVDVYGALTADGSRVILYPCGQQQNQQWTFYPDNTIRSLGKCLATSALSSGSNVVIT 362

Query: 361 NCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVE 420
           NCD     D  W VS SGT+MN SS+LVLTAN  T  TNLT E N +AA Q WR+G+YVE
Sbjct: 363 NCDYLRYDD-GWMVSSSGTMMNKSSHLVLTANAATSRTNLTGENNVFAAKQAWRIGNYVE 422

Query: 421 PIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLN 469
           PI+T+IIGL+ MCLEAT+N+TN+WLE CVKNK +Q WALYSD TIRVNNNR+LCV+SS +
Sbjct: 423 PIVTTIIGLRHMCLEATDNDTNVWLESCVKNKTKQYWALYSDDTIRVNNNRNLCVSSSTD 482

[Close]

BLAST of Sgr019857 vs. ExPASy TrEMBL
Match: A0A0A0KN71 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 5.7e-134
Identity = 268/511 (52.45%), Postives = 336/511 (65.75%), Query Frame = 0


Query: 1   MRVLVLCIVVALSLATHGIEANLCLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPVLD 60
           MRVL L  +V LS+AT+ +     +       D Y+S+I  +RQ+   + + LYGIPVL 
Sbjct: 1   MRVL-LAFIVTLSIATNALSKRPVV-------DVYQSYIQTIRQKFGSKTHKLYGIPVLH 60

Query: 61  HSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAG-SESYFFPNAPQVAFHIIFSN 120
           HS+SNS RFH     N   +TI  A+D +D  VVAY AG ++SYFF NAP+ AF I+F  
Sbjct: 61  HSLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPK 120

Query: 121 TNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVI 180
           TN+NLLNF+N+F+SIE AANTTR  T LG+ P  +AI+NLF Y     PVS L+V QMV 
Sbjct: 121 TNQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVF 180

Query: 181 EASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFG------- 240
           E++K+KFIEQ I NSI +   FTP LA++SLE+NWSELSL+IQAS SLQGLFG       
Sbjct: 181 ESAKFKFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYN 240

Query: 241 ---------------------------NAWHWRQCP-------KHDPCYVEARTTRISGR 300
                                      N   + + P       ++  CYV+ RT RISG+
Sbjct: 241 SKNEAVEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQ 300

Query: 301 EGFCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIY 360
           +G CADVA     DGS +I  PCGQQANQ+WTFH D TIRS  KCL  N S      VI 
Sbjct: 301 DGLCADVA----RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQ 360

Query: 361 NCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVE 420
           NC+K +Q+D TWDVSISGTIMNP+ +LVLT+  G    +L+++ N Y  +QGWRVG+YVE
Sbjct: 361 NCNKVSQEDATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVE 420

Query: 421 PIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLN 470
           PII SIIG+K+MCLEATE NTN+WLEECVKNK+EQSWA++SDG+IRVNN+ SLC+T+S  
Sbjct: 421 PIIASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSI 480

[Close]

BLAST of Sgr019857 vs. ExPASy TrEMBL
Match: A0A5A7V3Q8 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G00010 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.4e-132
Identity = 266/511 (52.05%), Postives = 335/511 (65.56%), Query Frame = 0


Query: 1   MRVLVLCIVVALSLATHGIEANLCLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPVLD 60
           MRVL L  VV LS AT+ +     +       D Y+S+I  +RQ+   + + LYGIPVL 
Sbjct: 1   MRVL-LAFVVTLSFATNALSKRPVV-------DVYQSYIQTIRQKFGSKTHKLYGIPVLQ 60

Query: 61  HSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGS-ESYFFPNAPQVAFHIIFSN 120
           HS+SNS RFH     N   +TI LA+D +D  VVAY AG+ +SYFF NAP+ AF I+F N
Sbjct: 61  HSLSNSDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPN 120

Query: 121 TNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVI 180
           TN+NLLNF+++F+SIEKAANTTR  T LG     +AI+NLF Y     PVS L+V QM+ 
Sbjct: 121 TNQNLLNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIF 180

Query: 181 EASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFG------- 240
           E++K+ FIEQ I NSI+    FTP LA++SLE+NWSELSLQIQAS SLQGLFG       
Sbjct: 181 ESAKFNFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYN 240

Query: 241 ---------------------------NAWHWRQCP-------KHDPCYVEARTTRISGR 300
                                      N   + + P       ++  CYV+ RT RISGR
Sbjct: 241 SKNEAIEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISGR 300

Query: 301 EGFCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIY 360
           +G C DVA     DGS++I  PCGQQANQ+WTF+ D TIRS  KCL    S    + VI 
Sbjct: 301 DGLCVDVA----RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKANPFAVIL 360

Query: 361 NCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVE 420
           +C+K +Q+D TWDVSISGTIMNP+ +LVLT+N G    +L+++ N Y  +QGWRVG++VE
Sbjct: 361 DCNKVSQQDATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVE 420

Query: 421 PIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLN 470
           P+I SIIG+K+MCLEATE NTN+WLEECVKNK+EQSWA+YSDG+IRVNN+ SLCVT+S  
Sbjct: 421 PLIVSIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSI 480

[Close]

BLAST of Sgr019857 vs. ExPASy TrEMBL
Match: A0A1S3CIM5 (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.4e-132
Identity = 266/511 (52.05%), Postives = 335/511 (65.56%), Query Frame = 0


Query: 1   MRVLVLCIVVALSLATHGIEANLCLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPVLD 60
           MRVL L  VV LS AT+ +     +       D Y+S+I  +RQ+   + + LYGIPVL 
Sbjct: 1   MRVL-LAFVVTLSFATNALSKRPVV-------DVYQSYIQTIRQKFGSKTHKLYGIPVLQ 60

Query: 61  HSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGS-ESYFFPNAPQVAFHIIFSN 120
           HS+SNS RFH     N   +TI LA+D +D  VVAY AG+ +SYFF NAP+ AF I+F N
Sbjct: 61  HSLSNSDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPN 120

Query: 121 TNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVI 180
           TN+NLLNF+++F+SIEKAANTTR  T LG     +AI+NLF Y     PVS L+V QM+ 
Sbjct: 121 TNQNLLNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIF 180

Query: 181 EASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFG------- 240
           E++K+ FIEQ I NSI+    FTP LA++SLE+NWSELSLQIQAS SLQGLFG       
Sbjct: 181 ESAKFNFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYN 240

Query: 241 ---------------------------NAWHWRQCP-------KHDPCYVEARTTRISGR 300
                                      N   + + P       ++  CYV+ RT RISGR
Sbjct: 241 SKNEAIEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISGR 300

Query: 301 EGFCADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIY 360
           +G C DVA     DGS++I  PCGQQANQ+WTF+ D TIRS  KCL    S    + VI 
Sbjct: 301 DGLCVDVA----RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKANPFAVIL 360

Query: 361 NCDKATQKDITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVE 420
           +C+K +Q+D TWDVSISGTIMNP+ +LVLT+N G    +L+++ N Y  +QGWRVG++VE
Sbjct: 361 DCNKVSQQDATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVE 420

Query: 421 PIITSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLN 470
           P+I SIIG+K+MCLEATE NTN+WLEECVKNK+EQSWA+YSDG+IRVNN+ SLCVT+S  
Sbjct: 421 PLIVSIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSI 480

[Close]

BLAST of Sgr019857 vs. ExPASy TrEMBL
Match: A0A5D3D0Q4 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G00170 PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 4.5e-131
Identity = 259/510 (50.78%), Postives = 335/510 (65.69%), Query Frame = 0


Query: 1   MRVLVLCIVVALSLATHGIEANL-CLSQAEFPADTYKSFILNLRQQLSFEANHLYGIPVL 60
           M  L   I+  LSLAT  I+ N   LS ++   D YK+FI  +RQ+L+   + LY IP+L
Sbjct: 1   MTSLTFYIIAFLSLATRRIDGNQHSLSSSDLLVDGYKNFINVIRQRLTENTSKLYDIPIL 60

Query: 61  DHSVSNSRRFHSTHLTNANDETIILAIDVEDAGVVAYQAGSESYFFPNAPQVAFHIIFSN 120
             S+  ++RF + ++ N NDETI LAID  + GV+ Y++ + SY F +AP VAF I+F  
Sbjct: 61  KSSLPPTQRFMTINIVNQNDETISLAIDKVNLGVLGYRSNNTSYIFLDAPLVAFDIVFPK 120

Query: 121 TNRNLLNFNNTFQSIEKAANTTRLETLLGVDPLESAISNLFQYRREFVPVSLLVVIQMVI 180
           T R LL FN+ ++SIEKA+ TTRL+TLLG++PL SAISNLF Y R  +P S LV++QMV+
Sbjct: 121 TCRVLLGFNSNYESIEKASGTTRLQTLLGLEPLNSAISNLFHYHRASIPESFLVILQMVL 180

Query: 181 EASKYKFIEQSITNSIRDRYNFTPGLAIVSLENNWSELSLQIQASMSLQGLFGN------ 240
           E SK+KFIE+S+  S++  YNF PGLAIVSLE+NW +LS Q+QAS SLQGLFG       
Sbjct: 181 EGSKFKFIERSVIQSLKYGYNFKPGLAIVSLEDNWEKLSSQVQASPSLQGLFGEAIKLYD 240

Query: 241 --------------------AWHWRQC-----------PKHDPCYVEARTTRISGREGFC 300
                               A+    C              D C V+ RT  I+G+ GFC
Sbjct: 241 SNNKVIEVDSIYYAIITTNIAFQLHHCNISTNFIRMPSDVGDSCNVQTRTATITGQNGFC 300

Query: 301 ADVAGELSADGSRLILYPCGQQANQKWTFHSDQTIRSLGKCLTTNTSSFGNYLVIYNCDK 360
            D +  L  D + +ILY CG Q+NQ+WTF SD+TIR   KCLT +TS F   +V+YNC +
Sbjct: 301 VDASNGLHQDDNSIILYRCGYQSNQEWTFLSDKTIRYSNKCLTFDTSRF---VVLYNCSE 360

Query: 361 ATQK-DITWDVSISGTIMNPSSNLVLTANKGTRSTNLTVEANTYAASQGWRVGDYVEPII 420
             +K +I W+V+I GTI NPSS LVLT N  T S+ L VE N +  SQGWRVG+YVEP+ 
Sbjct: 361 VEKKSNIRWNVAIDGTISNPSSGLVLTTNPSTNSSQLIVEVNKFTTSQGWRVGNYVEPLT 420

Query: 421 TSIIGLKEMCLEATENNTNMWLEECVKNKMEQSWALYSDGTIRVNNNRSLCVTSSLNSSS 472
            SIIGL+EMCLEAT NNTN+WLE+CVKNK EQ WA+Y DG+IRVN+ R+LCV+SS N   
Sbjct: 421 GSIIGLEEMCLEATNNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLCVSSSSNRIL 480

The following BLAST results are available for this feature:

BLAST of Sgr019857 vs. NCBI nr


Match Name	E-value	Identity	Description
U3KRF8.2	7.1e-155	60.66	RecName: Full=Seed lectin; Short=SGSL; Contains: RecName: Full=Seed lectin Aalph...	[more]
XP_022156703.1	1.4e-147	55.88	seed lectin-like [Momordica charantia] >BAH05018.1 galactose-binding type-2 ribo...	[more]
XP_038884532.1	8.7e-137	53.83	seed lectin-like [Benincasa hispida]	[more]
XP_004140357.1	1.2e-133	52.45	seed lectin [Cucumis sativus] >KGN51070.1 hypothetical protein Csa_008446 [Cucum...	[more]
XP_038884101.1	1.3e-132	51.27	seed lectin-like [Benincasa hispida]	[more]

BLAST of Sgr019857 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
U3KRF8	9.3e-158	60.66	Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2	[more]
P33183	3.8e-95	39.77	Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2	[more]
Q41358	3.6e-85	37.80	Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1	[more]
O22415	6.1e-85	37.92	Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=...	[more]
P93543	1.1e-81	38.01	Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1	[more]

BLAST of Sgr019857 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
B7X8M2	6.9e-148	55.88	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MCL1 PE=1 SV=1	[more]
A0A0A0KN71	5.7e-134	52.45	rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1	[more]
A0A5A7V3Q8	1.4e-132	52.05	rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G0...	[more]
A0A1S3CIM5	1.4e-132	52.05	rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1	[more]
A0A5D3D0Q4	4.5e-131	50.78	rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G...	[more]

BLAST of Sgr019857 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 33..46 score: 39.66 coord: 171..191 score: 51.38 coord: 81..96 score: 44.57 coord: 205..222 score: 48.83 coord: 142..156 score: 42.11
IPR000772	Ricin B, lectin domain	SMART	SM00458	ricin_3	coord: 249..374 e-value: 4.7E-24 score: 95.9 coord: 378..471 e-value: 2.8E-6 score: 36.9
IPR000772	Ricin B, lectin domain	PFAM	PF00652	Ricin_B_lectin	coord: 253..371 e-value: 9.5E-22 score: 77.6 coord: 386..469 e-value: 2.8E-14 score: 53.5
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 377..472 score: 23.958
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 249..374 score: 28.522499
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 384..469 e-value: 8.12819E-14 score: 65.9898
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 254..371 e-value: 4.26262E-21 score: 86.7906
None	No IPR available	GENE3D	2.80.10.50		coord: 248..378 e-value: 5.9E-36 score: 125.6
None	No IPR available	GENE3D	2.80.10.50		coord: 379..471 e-value: 6.7E-34 score: 118.3
None	No IPR available	GENE3D	6.10.250.2640		coord: 21..63 e-value: 1.4E-8 score: 36.2
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 247..331 coord: 30..232 coord: 345..469
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 32..221 e-value: 3.7E-24 score: 85.4
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 247..331 coord: 30..232 coord: 345..469
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 67..184 e-value: 1.4E-24 score: 88.5
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 185..247 e-value: 1.5E-13 score: 52.4
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 28..235
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 245..373
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 380..469

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr019857.1	Sgr019857.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity