Sgr019711 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr019711 vs. NCBI nr
Match: XP_022150231.1 (isoamylase 2, chloroplastic [Momordica charantia])

HSP 1 Score: 1526.5 bits (3951), Expect = 0.0e+00
Identity = 753/885 (85.08%), Postives = 798/885 (90.17%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATF SSLV+QP+ VH  G+ E+F+LA SDHHT+GH +K+QSGK D+ RMLAHGES+  F
Sbjct: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
            K    NLSKAYA+  +S+EK+G RFG+S KSKE  KVATYLFRTE GDLVNV VRKT S
Sbjct: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
            F V IE+SS+Q  +++ETPMLSWGVYRSDSALVT N QSSLPD TIGT ETPFVNTSEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH+PFYLSFLLK+ VGVDSGSSEIRSHRKTSF VPVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGSMNFSIFSSSAESVVLCLY+DSTSEKPV+ELDLDRYINRSGNIWH SFE   NF S
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGY  KGSKSHK QD VE+SRILVDPYAK+L+TS  KTHGHGLGLPSKLLGQIC+VPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNL MEKLF Y+LNVKRFTVDKSSQL TDIAGTFAGLTKKLLHFKNLGVNAVLL
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIFPFDEKEGPYF SHFFSPTNIYGPSGGS SAINSMKEMVKELHANGIEVMLEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           TA+DG LQGIDDSS YF N+VAN EGKN LNCNYP+VQQLLLDSLRYWVTEFH+DGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NASSLLRGY GELLSRPPLVE IAFDPLLSKTKIIADFWDP E +SKE RFPHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           N KFC++VRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGL LVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           S KLASELSWNCGEEGPTSNIEVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           +A+ DRR FDWDLLK GFGTQTTQFI+FFSSFRARR DLFQSRNFLKGENIDWYGNN SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           PQWEDPSCKFLAVMLRADKEE G SAENP+P+ DIFMAFNASDQS +V LPEPSEG SWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846

Query: 841 RLIDSALSFPGFFSSDP--VPMTGSVTYEMQAHSCALFEAKSSKD 883
           RLID+ALSFPGFFSSD   VP   SVTYEMQAHSCALFEAKS  D
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891

BLAST of Sgr019711 vs. NCBI nr
Match: XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1486.5 bits (3847), Expect = 0.0e+00
Identity = 733/885 (82.82%), Postives = 790/885 (89.27%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATFLSSLVIQPFCV+  G+ ESFKLAV DHHTYG K+KY SGKMD+ARMLAHGES+V  
Sbjct: 1   MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
            K   RNLSK YATS + + KSGQRFG   KSKEH KVATYLFRTE+GDLVNVFV KT S
Sbjct: 61  TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
            F V +EISS+Q  S+++T +LSWGVYRSDSA+VT NFQSSLPD+TIG TETPFV TS G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH PFYLSFLLKY VGVDSGSSEIRSHRKTSF+VPVGFGRGYPSPLG+SI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGS+NFSIFSS+AESVVLCLY+DSTSEKPVLELDLD YINRSG+IWHASFE A NF S
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGYR KGSK H+NQD VEV+RI+VDPYAKIL+ S+ KT G GLGLP K LGQIC+VPAFD
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLGLP-KFLGQICKVPAFD 360

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLF Y+LNVKRFTVDKSSQLP DIAGTF+GLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIF FDEKEG YF  HFFSP N YGPSG S SAINSMK MVK+LHANG+EV+LEVVFTH
Sbjct: 421 EPIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTH 480

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           TA DGALQGIDDSS YF+N+VAN EGK+ LNCNYPIVQQLLLDSLRYWVTEFH+DGFCFI
Sbjct: 481 TAMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NASSLLRG+ GE LSR P VE IAFDPLLSKTK++ADFWDP+ELVS+E  FPHWKRWAEV
Sbjct: 541 NASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEV 600

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           NSKFCN+VRDFFRGEGL+SSLATRLCGSGD+FS+GRGPAFSFNFI RN GL LVDLVSFS
Sbjct: 601 NSKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFS 660

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           + KLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFV+FVSLGVPVLNMGDECGQS+GGS
Sbjct: 661 NSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGS 720

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           VA+NDRR F+WDLLK  FGTQTTQFI+F SSFR+RR DLFQSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           PQWEDPSCKFLAVMLRADKEE GS  EN KPR DIFMAFNASDQSE+V LPEPSEG SWF
Sbjct: 781 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWF 840

Query: 841 RLIDSALSFPGFFSSD--PVPMTGSVTYEMQAHSCALFEAKSSKD 883
           RL+D+ALSFPGFFSSD   VP TGSVTYEMQAHSCALFEAKS  D
Sbjct: 841 RLVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFND 884

BLAST of Sgr019711 vs. NCBI nr
Match: XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1477.6 bits (3824), Expect = 0.0e+00
Identity = 727/883 (82.33%), Postives = 788/883 (89.24%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATFLSSLVIQPFCVH CG+ ES KLAVSDH TYG K KYQSGKMD+ARMLAHGESKV  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
           +K   RNLSKAY TS + + KSGQR G+  KSKE  KVATYLFRTE+GDLVNVFV KT S
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
           +FTV IEISS+Q VS DET +LSW VYRSDSA VTSNFQSS PD T G TETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH PFYLSF+LK+ +GVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGS+NFSIFSS+AESVVLCLY+DSTSEKP+LELDLD YINRSGNIWHASFEGA NF S
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGY+ KG KSH+NQD +EVSRI+VDPYAKIL+ SIPK+   GLG P K LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+Y+LNVKRFT+DKSSQLP DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIF FDEKEGPYF  HFFSPTN YGPSG S SAINSMKEMVKELHANG+EV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           T+ DGALQGIDDSS YF N+VAN E ++ LNCNYPIVQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NAS LL+G+ GELLSRPP VE IAFDP+LSKTK++ADFWDP+ELVSKE RFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           NSKFC+++RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +  LASELSWNCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           VA+NDRR F+WDLLK  FGTQTTQFI+F SSFR+RR DLFQSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           PQWED SCKFLAVMLRADKEE  S  ENPK R DIFMAFNASDQSESV LPEPSEG SWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDSALSFPGFFSSDP--VPMTGSVTYEMQAHSCALFEAKSS 881
           R++D+AL FPGFFSSD   VPMTGSVTYEMQAHSCALFEAKS+
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSA 883

BLAST of Sgr019711 vs. NCBI nr
Match: KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 726/883 (82.22%), Postives = 787/883 (89.13%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATFLSSLVIQPFCVH CG+ ES KLAVSDH TYG K KYQSGKMD+ARMLAHGESKV  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
           +K   RNLSKAY TS +S+ KSGQR G+  KSKE  KVATYLFRTE+GDLVNVFV KT S
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
           +FTV IEISS+Q VS DET +LSW VYRSDSA VTSNFQSS PD T G TETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH PFYLSF+LK+ +GVDSGSSEIRSHRKTSFSVPVGFGRGYPSP G+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGS+NFSIFSS+AESVVLCLY+DSTSEKP+LELDLD YINRSGNIWHASFEG  NF S
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGY+ KG KSH+NQD +EVSRI+VDPYAKIL+ SIPK+   GLG P K LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+Y+LNVKRFT+DKSSQLP DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIF FDEKEGPYF  HFFSPTN YGPSG S SAINSMKEMVKELHANG+EV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           T+ DGALQGIDDSS YF N+VAN E ++ LNCNYPIVQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NAS LL+G+ GELLSRPP VE IAFDP+LSKTK++ADFWDP+ELVSKE RFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           NSKFC+++RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +  LASELSWNCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           VA+NDRR F+WDLLK  FGTQTTQFI+F SSFR+RR DLFQSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           PQWED SCKFLAVMLRADKEE  S  ENPK R DIFMAFNASDQSESV LPEPSEG SWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDSALSFPGFFSSDP--VPMTGSVTYEMQAHSCALFEAKSS 881
           R++D+AL FPGFFSSD   VPMTGSVTYEMQAHSCALFEAKS+
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSA 883

BLAST of Sgr019711 vs. NCBI nr
Match: XP_004136188.1 (isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein Csa_016566 [Cucumis sativus])

HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 718/885 (81.13%), Postives = 788/885 (89.04%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATFLSSLVIQPFCV+ CG+ ES KLA SDH TYG K KYQ GKMD+ARMLAHGE+KV  
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
           +K   RNLSK YA S +S+ KSGQR G+  KSKE  +VATYLFRTE+GDLVNVFV K  S
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
           +FTV IE+ S+Q VS+DE  +LSWGVYRSDSALVT NF+SS PD+T G TETPFV TSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH PFYLSF+LKY +GVDSGSSEIRSH+KTSFSVPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGS+NFSIFSSSAES+VLCLY+DSTSEKP+LELDLD YINRSGNIWHASFEGA  F S
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGY+ KGSKSH+NQD +EVSRI+VDPYAKIL+ SIPK+ G GLGLPSK LGQI +VP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLF+Y+LNV+RFT+DKSSQLP DIAGTF+GLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIF FDEKEGPYF  HFFSPTN YGPSG S SAINSMKEMVKELHANG+EV+LEVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           T+ +GALQGIDDSS YF N+VAN E K+ LNCNYPIVQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NAS LLRG+ GELLSRPP VE IAFDPLLSKTK++ADFWDP+EL SKE RFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           NSKFC+++RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +  LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           VA+ND+R F+WDLLK  FGTQTTQFI+F SSFR+RR DLFQ+RNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           PQWED SCKFLAVMLRADKEE  S  ENPK R +IFM FNASDQSESV LPEP EG SWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RLIDSALSFPGFFSSDP--VPMTGSVTYEMQAHSCALFEAKSSKD 883
           R++D+AL FPGFFSSD   VPMTGSVTYE+QAHSCALFEAKS+ D
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of Sgr019711 vs. ExPASy Swiss-Prot
Match: Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 927.5 bits (2396), Expect = 1.1e-268
Identity = 460/815 (56.44%), Postives = 595/815 (73.01%), Query Frame = 0

Query: 75  SRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRSSFTVCIEISSLQPV 134
           S+ SIE         +++ +  KV +Y FRT+ G LV V V K R  +++ + +SSL+  
Sbjct: 72  SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLELS 131

Query: 135 SVDETPM-LSWGVYRSDSA-LVTSNFQSSLPDKTIGTTETPFVNTSEGKFVVELEFDAKH 194
             D++ + + WGVYRSDS+  +  +F++S  D    TTET FV +S  + ++ LEFD K 
Sbjct: 132 GDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKE 191

Query: 195 IPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISIS-SDGSMNFSIF 254
            PFYLSF LK   G D    E+ +HR T F +PVGF  G+P PLG+S    D S NFS F
Sbjct: 192 SPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFF 251

Query: 255 SSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSSYGYRFKGSKSH 314
           S S+ +VVLCLYDDST++KP LELDLD Y+NR+G++WHAS +   +F  YGYR K +   
Sbjct: 252 SRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHS 311

Query: 315 KNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFDWDGEVRPNLPM 374
           K   +VE   I++DPYA ++  S+ +          K LG + + P+FDW  +V PN+P+
Sbjct: 312 KEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDVSPNIPL 371

Query: 375 EKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLLEPIFPFDEKEG 434
           EKL +Y+LNVK FT  +SS+LP+++AGTF+G+ +K+ H K LG NAVLLEPIF F E++G
Sbjct: 372 EKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKG 431

Query: 435 PYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTHTAKDGALQGID 494
           PYF  HFFSP +IYGPS    SA+NSMK MVK+LH+ GIEV+LEVVFTHTA  GAL+GID
Sbjct: 432 PYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGID 491

Query: 495 DSSYFMNQVANS-EGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFINASSLLRGYDG 554
           DSSY+    AN  + K+ LNCNYP+VQQL+L+SLRYWVTEFHVDGFCFINASSLLRG  G
Sbjct: 492 DSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHG 551

Query: 555 ELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEVNSKFCNNVRDF 614
           E LSRPPLVE IAFDPLL++TK+IAD WDP E++ KE+RFPHWKRWAE+N+++C NVR+F
Sbjct: 552 EQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNF 611

Query: 615 FRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFSSGKLASELSWN 674
            RG G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN GL+LVD+VSFS  +LASELSWN
Sbjct: 612 LRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWN 671

Query: 675 CGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAYNDRRLFDW 734
           CGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     R+ FDW
Sbjct: 672 CGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDW 731

Query: 735 DLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSPPQWEDPSCKFL 794
           +LL   FGTQ TQFISF +S RARRSD+FQ R+FLK ENI WY N+Q+ P+WEDP+ KFL
Sbjct: 732 NLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFL 791

Query: 795 AVMLR--ADKEETGSSAENPKPR-GDIFMAFNASDQSESVTLPEPSEGMSWFRLIDSALS 854
           A+ ++  +++EET S AE  +P+  D+F+ FNASD  ESV LP   +G  W RL+D+AL 
Sbjct: 792 ALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALP 851

Query: 855 FPGFFSSD------PVPMTGSVTYEMQAHSCALFE 877
           FPGFFS +        P+   V YEM+ +SC LFE
Sbjct: 852 FPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Sgr019711 vs. ExPASy Swiss-Prot
Match: Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 1.7e-213
Identity = 370/714 (51.82%), Postives = 498/714 (69.75%), Query Frame = 0

Query: 185 ELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISISSDG 244
           EL FD    PFYLSFLL      D+  +EIR+HRKTSF VPVG G G P+PLG+SIS DG
Sbjct: 107 ELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPLGMSISGDG 166

Query: 245 SMNFSIFSSSAESVVLCLYDDST----SEKPVLELDLDRYINRSGNIWHASFEGALNFSS 304
           ++NF+++S +A +V L LY  +      ++P LE+DLD YI+R+GN+WH S      + S
Sbjct: 167 AVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSLASVDGYVS 226

Query: 305 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILS--TSIPKTHGHGLGLPS-KLLGQICQVP 364
           Y +   G           + R L+DPYAK++    S    +  G+  PS +    +   P
Sbjct: 227 YAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASLAIAP 286

Query: 365 AFDWDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNA 424
           +++W  +  P LP+EKL +Y+ NV  FT D+SS LP D AGTF GL+ K+ HF++LGVNA
Sbjct: 287 SYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGVNA 346

Query: 425 VLLEPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVV 484
           +LLEP+FPF + +GPYF  HFFSP N+Y   G S SAI SMK+MV+ +H NGIEV+LEVV
Sbjct: 347 ILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLEVV 406

Query: 485 FTHTAKDG------ALQGIDDSSYFM-NQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTE 544
           FTHTA+        +++GID+SSY++ N +A  +  + LNCN+P+ Q+L+LDSLR+WV +
Sbjct: 407 FTHTAEGESECQTISMRGIDNSSYYIANGIAGCKA-SILNCNHPVTQKLILDSLRHWVLD 466

Query: 545 FHVDGFCFINASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRF 604
           FHVDGFCFINA  L+RG  GE LSRPPL+E I FDP+LS TKIIAD W P ++ + +  F
Sbjct: 467 FHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNVQFPF 526

Query: 605 PHWKRWAEVNSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGL 664
           PHWKRWAEVN++F  +VR F + E L+S LATRLCGSGD+FS  RGPAFSFN ++RN GL
Sbjct: 527 PHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSRNSGL 586

Query: 665 TLVDLVSFSSGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGD 724
           +LVDLVSFS+  L SE SWNCGEEGP+ N  VL+ RL+QIRNF+F+LFVSLGVPVLNMGD
Sbjct: 587 SLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNMGD 646

Query: 725 ECGQSSGGSVAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENI 784
           ECG S+ GSV+Y DR   +W  +K  F  + T FISF ++ R+RR D+FQ R FLK ENI
Sbjct: 647 ECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLENI 706

Query: 785 DWYGNNQSPPQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLP 844
            WYG++   P W+DP+  FL + + A+ +E  + +     RGD+++ FNA+++S S  LP
Sbjct: 707 HWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAADS----VRGDLYICFNANEESVSAALP 766

Query: 845 EPSEGMSWFRLIDSALSFPGFFSSDPVP----MTGSVTYEMQAHSCALFEAKSS 881
             +EG  W RL+D++L+FPGFF+++  P    + G  +Y ++AH+C LFE+KS+
Sbjct: 767 ALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESKSA 798

BLAST of Sgr019711 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 6.9e-106
Identity = 258/722 (35.73%), Postives = 378/722 (52.35%), Query Frame = 0

Query: 212 SEIRSHRKTSFSVPVGFGRGYPSPLGISISSDGSMNFSIFSSSAESVVLCLY-----DDS 271
           +E R    +   +P  +  G   PLG+S   +G +NF++FS  A SV+LCL       + 
Sbjct: 84  TEERGCTMSDTEMPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTED 143

Query: 272 TSEKPVLELDLDRYINRSGNIWHASFEG-ALNFSSYGYRFKGSKSHKNQDEVEVSRILVD 331
                V+E  LD+  N++G+IWH   EG   +   YGYR  G +        + S +L+D
Sbjct: 144 EKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLD 203

Query: 332 PYAKILSTSIPKTHGHGLGLPSKLLGQI-CQVPAFDWDGEVR-PNLPMEKLFIYKLNVKR 391
           PYAK++S    K  G      S+  G        FDW  + R PNLP   L IY++NV+ 
Sbjct: 204 PYAKLVSGR--KYFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRA 263

Query: 392 FTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLLEPIFPFDEKE------------- 451
           FT D+SS L +   G++ GL  K+ H   LGVNAV L P+F +DE E             
Sbjct: 264 FTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVN 323

Query: 452 -GPYFSSHFFSPTNIYGPSGGS-TSAINSMKEMVKELHANGIEVMLEVVFTHT--AKDG- 511
              Y + +FF+P + Y  +GG   +A   +K+MVKELH  GIEV+L+VV+ HT  A D  
Sbjct: 324 TWGYSTINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAH 383

Query: 512 ----ALQGIDDSSYFM------NQVANSEG-KNTLNCNYPIVQQLLLDSLRYWVTEFHVD 571
               + +GID+  Y+M       ++ N  G  NTLNCN+P+V++L+LDSLR+WV E+H+D
Sbjct: 384 PYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHID 443

Query: 572 GFCFINASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWK 631
           GF F  AS L RG DG  L  PPL++ IA D +LS+ KIIA+ WD   L     RFP+W 
Sbjct: 444 GFRFDLASVLCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLYLVG-RFPNWD 503

Query: 632 RWAEVNSKFCNNVRDFFRGE-GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNFGLTL 691
           RWAE N K+ +++R F +G+ G+    ATR+ GS D++  + R P    NF+  + G TL
Sbjct: 504 RWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTL 563

Query: 692 VDLVSF------SSGK-----LASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSL 751
            DLVS+      ++G+          SWNCG EG T+++ VL  R +Q++NF   L +S 
Sbjct: 564 CDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQ 623

Query: 752 GVPVLNMGDECGQSS-GGSVAYNDRRL---FDWDLLKRGFGTQTTQFISFFSSFRARRSD 811
           G P++ MGDE G +  G + +Y        F W+ L++    +   F  F    + R S+
Sbjct: 624 GTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQ---RRDGHFRFFSEMIKFRHSN 683

Query: 812 -LFQSRNFLKGENIDWYGNNQSPPQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFM 871
            + +   FL   ++ W+ +      WE+   KFLA            +  +    GDI++
Sbjct: 684 PILRRDRFLNKNDVTWHED-----CWENQESKFLAF-----------TVHDHNSGGDIYL 743

Query: 872 AFNASDQSESVTLPEPSEGMSWFRLIDSALSFPGFFSSDPVPMTGSVTYEMQAHSCALFE 879
           AFNA D      +P P     W R++D+ L  P     + VP TG   Y +  +S  L +
Sbjct: 744 AFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEGVPFTGP-KYRIAPYSSILLK 781

BLAST of Sgr019711 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 384.0 bits (985), Expect = 4.5e-105
Identity = 259/706 (36.69%), Postives = 371/706 (52.55%), Query Frame = 0

Query: 231 GYPSPLGISISSDGSMNFSIFSSSAESVVLCLY-----DDSTSEKPVLELDLDRYINRSG 290
           G  SPLG+S   D  +NF++FS +A SV LCL       D T +  ++EL LD  +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEG-ALNFSSYGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGH-GL 350
           + WH   E   LN   YGYR  G    +     + S +L+DPYAK++        GH   
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVK-------GHSSF 207

Query: 351 GLPSKLLGQI-----CQVPAFDWDGEVR-PNLPMEKLFIYKLNVKRFTVDKSSQLPTDIA 410
           G  S+   Q       +   FDW  + + PN+P + L IY++NV+ FT D+SS +   I 
Sbjct: 208 GDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIG 267

Query: 411 GTFAGLTKKLLHFKNLGVNAVLLEPIFPFDEKE--------------GPYFSSHFFSPTN 470
           G++ G  +K+ H ++LG+NAV L P+F FDE E                Y + +FF+P +
Sbjct: 268 GSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMS 327

Query: 471 IYGP-SGGSTSAINSMKEMVKELHANGIEVMLEVVFTHTAK-------DGALQGIDDSSY 530
            Y    G    A    KEMVK LH+ GIEV+L+VV+ HT +         + +GID+  Y
Sbjct: 328 RYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVY 387

Query: 531 FM----NQVANSEG-KNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFINASSLLRGYDG 590
           +M    NQ+ N  G  NTLNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L R  DG
Sbjct: 388 YMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDG 447

Query: 591 ELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEVNSKFCNNVRDF 650
             LS PPL+  IA D +LS+ KIIA+ WD   L     +FP+W RWAE N  + ++VR F
Sbjct: 448 SPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVG-KFPNWDRWAEWNGMYRDDVRRF 507

Query: 651 FRGE-GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNFGLTLVDLVSF------SSGK 710
            +G+ G+  S ATR+ GS D++  + R P    NF+  + G TL DLVS+      ++G+
Sbjct: 508 IKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGE 567

Query: 711 LASE-----LSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS- 770
             ++      SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  
Sbjct: 568 GGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRY 627

Query: 771 GGSVAY-NDRRL--FDWDLLKRGFGTQTTQFISFFSSFRARRS-DLFQSRNFLKGENIDW 830
           G + +Y +D  L  F W  L      +   F  F    + R S  + +  NFL    I W
Sbjct: 628 GNNNSYGHDTSLNNFQWKELD---AKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITW 687

Query: 831 YGNNQSPPQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEP 879
           + +N     W++   KFLA  L    +  G          DI++AFNA D      +P+P
Sbjct: 688 HEDN-----WDNSESKFLAFTLH---DGIGGR--------DIYVAFNAHDYFVKALIPQP 747

BLAST of Sgr019711 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.9e-103
Identity = 250/695 (35.97%), Postives = 363/695 (52.23%), Query Frame = 0

Query: 231 GYPSPLGISISSDGSMNFSIFSSSAESVVLCLY--DDSTSEKPVLELDLDRYINRSGNIW 290
           G P+PLG + + DG +NF+++S+ A +  LCL+  DD  +++   E+ LD   NR+GN+W
Sbjct: 99  GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158

Query: 291 HASFEGALNFSSYGYRFKGSKSHKNQDEVEVSRILVDPYAK-ILSTSIPKTHGHGLGLPS 350
           H   EG L+   YGYRF G  +       +VS ++VDPYAK ++S       G G     
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218

Query: 351 KLLGQI-CQVPAFDWDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKK 410
           ++ G I      FDW G++    P + L IY+++++ FT   SS +  +  GT+ G   K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNV--EHPGTYIGAISK 278

Query: 411 LLHFKNLGVNAVLLEPIFPFDEKEGPYFSS------------HFFSPTNIYGPSGG---- 470
           L + K LGVN V L P   F+E E  YFS             +FFSP   Y  SGG    
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELE--YFSCSSKMNFWGYSTINFFSPMIRYS-SGGIRNC 338

Query: 471 STSAINSMKEMVKELHANGIEVMLEVVFTHTAKDG------ALQGIDDSSYFM----NQV 530
              AIN  K  V+E H  GIEV+++VVF HTA+        + +GID+S+Y+M     + 
Sbjct: 339 GRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEF 398

Query: 531 ANSEG-KNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFINASSLLRGYD---------- 590
            N  G  NT NCN+P+V++ ++D LRYWVTE HVDGF F  AS + RG            
Sbjct: 399 YNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGS 458

Query: 591 ---------GELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEVN 650
                    G  L+ PPL+++I+ DP+L   K+IA+ WD   L     +FPHWK W+E N
Sbjct: 459 PVEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLYQVG-QFPHWKIWSEWN 518

Query: 651 SKFCNNVRDFFRG-EGLVSSLATRLCGSGDIF-SDGRGPAFSFNFIARNFGLTLVDLVSF 710
            K+ + VR F +G +G     A  LCGS  ++ + GR P  S NF+  + G TL DLV++
Sbjct: 519 GKYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTY 578

Query: 711 SSGKLAS-----------ELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLN 770
           +    +S            LSWNCGEEG  + + V   R +Q+RNF   L VS GVP+  
Sbjct: 579 NKKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFY 638

Query: 771 MGDECGQSSGG--SVAYNDR--RLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRN 830
           MGDE G + GG  +   +D     F WD  K+   +   +F S  + FR ++ +     +
Sbjct: 639 MGDEYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLAD 698

Query: 831 FLKGENIDWYGNNQSPPQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQ 859
           F   + + W+G+    P W + S +F+A    + K+ET         +G+I++AFNAS  
Sbjct: 699 FPTAQRLHWHGHQPGKPDWSETS-RFVAF---STKDET---------KGEIYVAFNASHL 758

BLAST of Sgr019711 vs. ExPASy TrEMBL
Match: A0A6J1DAX4 (isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 SV=1)

HSP 1 Score: 1526.5 bits (3951), Expect = 0.0e+00
Identity = 753/885 (85.08%), Postives = 798/885 (90.17%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATF SSLV+QP+ VH  G+ E+F+LA SDHHT+GH +K+QSGK D+ RMLAHGES+  F
Sbjct: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
            K    NLSKAYA+  +S+EK+G RFG+S KSKE  KVATYLFRTE GDLVNV VRKT S
Sbjct: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
            F V IE+SS+Q  +++ETPMLSWGVYRSDSALVT N QSSLPD TIGT ETPFVNTSEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH+PFYLSFLLK+ VGVDSGSSEIRSHRKTSF VPVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGSMNFSIFSSSAESVVLCLY+DSTSEKPV+ELDLDRYINRSGNIWH SFE   NF S
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGY  KGSKSHK QD VE+SRILVDPYAK+L+TS  KTHGHGLGLPSKLLGQIC+VPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNL MEKLF Y+LNVKRFTVDKSSQL TDIAGTFAGLTKKLLHFKNLGVNAVLL
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIFPFDEKEGPYF SHFFSPTNIYGPSGGS SAINSMKEMVKELHANGIEVMLEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           TA+DG LQGIDDSS YF N+VAN EGKN LNCNYP+VQQLLLDSLRYWVTEFH+DGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NASSLLRGY GELLSRPPLVE IAFDPLLSKTKIIADFWDP E +SKE RFPHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           N KFC++VRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGL LVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           S KLASELSWNCGEEGPTSNIEVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           +A+ DRR FDWDLLK GFGTQTTQFI+FFSSFRARR DLFQSRNFLKGENIDWYGNN SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           PQWEDPSCKFLAVMLRADKEE G SAENP+P+ DIFMAFNASDQS +V LPEPSEG SWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846

Query: 841 RLIDSALSFPGFFSSDP--VPMTGSVTYEMQAHSCALFEAKSSKD 883
           RLID+ALSFPGFFSSD   VP   SVTYEMQAHSCALFEAKS  D
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891

BLAST of Sgr019711 vs. ExPASy TrEMBL
Match: A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1477.6 bits (3824), Expect = 0.0e+00
Identity = 727/883 (82.33%), Postives = 788/883 (89.24%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATFLSSLVIQPFCVH CG+ ES KLAVSDH TYG K KYQSGKMD+ARMLAHGESKV  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
           +K   RNLSKAY TS + + KSGQR G+  KSKE  KVATYLFRTE+GDLVNVFV KT S
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
           +FTV IEISS+Q VS DET +LSW VYRSDSA VTSNFQSS PD T G TETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH PFYLSF+LK+ +GVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGS+NFSIFSS+AESVVLCLY+DSTSEKP+LELDLD YINRSGNIWHASFEGA NF S
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGY+ KG KSH+NQD +EVSRI+VDPYAKIL+ SIPK+   GLG P K LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+Y+LNVKRFT+DKSSQLP DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIF FDEKEGPYF  HFFSPTN YGPSG S SAINSMKEMVKELHANG+EV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           T+ DGALQGIDDSS YF N+VAN E ++ LNCNYPIVQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NAS LL+G+ GELLSRPP VE IAFDP+LSKTK++ADFWDP+ELVSKE RFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           NSKFC+++RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +  LASELSWNCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           VA+NDRR F+WDLLK  FGTQTTQFI+F SSFR+RR DLFQSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           PQWED SCKFLAVMLRADKEE  S  ENPK R DIFMAFNASDQSESV LPEPSEG SWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDSALSFPGFFSSDP--VPMTGSVTYEMQAHSCALFEAKSS 881
           R++D+AL FPGFFSSD   VPMTGSVTYEMQAHSCALFEAKS+
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSA 883

BLAST of Sgr019711 vs. ExPASy TrEMBL
Match: A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 726/883 (82.22%), Postives = 787/883 (89.13%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATFLSSLVIQPFCVH CG+ ES KLAVSDH TYG K KYQSGKMD+ARMLAHGESKV  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
           +K   RNLSKAY TS +S+ KSGQR G+  KSKE  KVATYLFRTE+GDLVNVFV KT S
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
           +FTV IEISS+Q VS DET +LSW VYRSDSA VTSNFQSS PD T G TETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH PFYLSF+LK+ +GVDSGSSEIRSHRKTSFSVPVGFGRGYPSP G+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGS+NFSIFSS+AESVVLCLY+DSTSEKP+LELDLD YINRSGNIWHASFEG  NF S
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGY+ KG KSH+NQD +EVSRI+VDPYAKIL+ SIPK+   GLG P K LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+Y+LNVKRFT+DKSSQLP DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIF FDEKEGPYF  HFFSPTN YGPSG S SAINSMKEMVKELHANG+EV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           T+ DGALQGIDDSS YF N+VAN E ++ LNCNYPIVQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NAS LL+G+ GELLSRPP VE IAFDP+LSKTK++ADFWDP+ELVSKE RFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           NSKFC+++RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +  LASELSWNCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           VA+NDRR F+WDLLK  FGTQTTQFI+F SSFR+RR DLFQSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           PQWED SCKFLAVMLRADKEE  S  ENPK R DIFMAFNASDQSESV LPEPSEG SWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDSALSFPGFFSSDP--VPMTGSVTYEMQAHSCALFEAKSS 881
           R++D+AL FPGFFSSD   VPMTGSVTYEMQAHSCALFEAKS+
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSA 883

BLAST of Sgr019711 vs. ExPASy TrEMBL
Match: A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 718/885 (81.13%), Postives = 788/885 (89.04%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATFLSSLVIQPFCV+ CG+ ES KLA SDH TYG K KYQ GKMD+ARMLAHGE+KV  
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
           +K   RNLSK YA S +S+ KSGQR G+  KSKE  +VATYLFRTE+GDLVNVFV K  S
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
           +FTV IE+ S+Q VS+DE  +LSWGVYRSDSALVT NF+SS PD+T G TETPFV TSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH PFYLSF+LKY +GVDSGSSEIRSH+KTSFSVPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGS+NFSIFSSSAES+VLCLY+DSTSEKP+LELDLD YINRSGNIWHASFEGA  F S
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGY+ KGSKSH+NQD +EVSRI+VDPYAKIL+ SIPK+ G GLGLPSK LGQI +VP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLF+Y+LNV+RFT+DKSSQLP DIAGTF+GLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIF FDEKEGPYF  HFFSPTN YGPSG S SAINSMKEMVKELHANG+EV+LEVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           T+ +GALQGIDDSS YF N+VAN E K+ LNCNYPIVQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NAS LLRG+ GELLSRPP VE IAFDPLLSKTK++ADFWDP+EL SKE RFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           NSKFC+++RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +  LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           VA+ND+R F+WDLLK  FGTQTTQFI+F SSFR+RR DLFQ+RNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           PQWED SCKFLAVMLRADKEE  S  ENPK R +IFM FNASDQSESV LPEP EG SWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RLIDSALSFPGFFSSDP--VPMTGSVTYEMQAHSCALFEAKSSKD 883
           R++D+AL FPGFFSSD   VPMTGSVTYE+QAHSCALFEAKS+ D
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of Sgr019711 vs. ExPASy TrEMBL
Match: A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1460.7 bits (3780), Expect = 0.0e+00
Identity = 721/885 (81.47%), Postives = 783/885 (88.47%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHYCGINESFKLAVSDHHTYGHKMKYQSGKMDDARMLAHGESKVSF 60
           MATFLSSL IQPFC +  G  ESFKLAVSD+HTYG KM YQSGK+D+ARM AHGESKV F
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  LKYPPRNLSKAYATSRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRS 120
            K   RN+ +AYA SR+S+ KSGQ+FG   K++E  KVATYLFRTE+GDLV  FV K  S
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 SFTVCIEISSLQPVSVDETPMLSWGVYRSDSALVTSNFQSSLPDKTIGTTETPFVNTSEG 180
            F   IEISSL+  S+DET +LSWGVYRS+SA+VT NFQS LP++TIG TETPFV TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFVVELEFDAKHIPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISI 240
           KF VELEFDAKH PFYLSFLLKY VGVDSGSSEI+SHRKTSFSVPVGFGRGYPSPLG+SI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSS 300
           S DGS+NFSIFSS+AESVVLCLY+DSTSEKPVLELDLD YINRSGNIWHASFEGA NF S
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFD 360
           YGYR +GSK H+NQDE EVSRILVDPYAKIL+ SIPKT G GLGLPSK LGQIC VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLFIY+LNVK FTVDKSSQLP D AGTF+GLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTH 480
           EPIFPFDEK+GPYF  HFFSPTNIY PSG S S++NS+KEMVKELHANG+EV+LEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 TAKDGALQGIDDSS-YFMNQVANSEGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFI 540
           TA DGALQGIDDS  YF+N+VANSEGK+ LNCN+PIVQQLLLDSLR+WVTEFH+DGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASSLLRGYDGELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEV 600
           NASSLL+G+ GELLSRPP VE IAFDPLLSKTKIIADFWDP+E V KE RFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCNNVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFS 660
           NSKF N++++FFRGEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SGKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           S KLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSP 780
           VA+ DR+ F+WD LK  FGTQTTQFI+F SSFR+RR DLFQSRNFLK ENIDW+ NNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEPSEGMSWF 840
           P WEDPSCKFLAVMLRADKEE+GS  ENPKP+ DIFMAFNASDQS SV LPEPSEG +WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RLIDSALSFPGFFSSD--PVPMTGSVTYEMQAHSCALFEAKSSKD 883
           RL+D+ALSFPGFFSSD  PVP T  VTY MQAHSC LFEAKS  D
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884

BLAST of Sgr019711 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 927.5 bits (2396), Expect = 7.7e-270
Identity = 460/815 (56.44%), Postives = 595/815 (73.01%), Query Frame = 0

Query: 75  SRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRSSFTVCIEISSLQPV 134
           S+ SIE         +++ +  KV +Y FRT+ G LV V V K R  +++ + +SSL+  
Sbjct: 72  SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLELS 131

Query: 135 SVDETPM-LSWGVYRSDSA-LVTSNFQSSLPDKTIGTTETPFVNTSEGKFVVELEFDAKH 194
             D++ + + WGVYRSDS+  +  +F++S  D    TTET FV +S  + ++ LEFD K 
Sbjct: 132 GDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKE 191

Query: 195 IPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISIS-SDGSMNFSIF 254
            PFYLSF LK   G D    E+ +HR T F +PVGF  G+P PLG+S    D S NFS F
Sbjct: 192 SPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFF 251

Query: 255 SSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSSYGYRFKGSKSH 314
           S S+ +VVLCLYDDST++KP LELDLD Y+NR+G++WHAS +   +F  YGYR K +   
Sbjct: 252 SRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHS 311

Query: 315 KNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFDWDGEVRPNLPM 374
           K   +VE   I++DPYA ++  S+ +          K LG + + P+FDW  +V PN+P+
Sbjct: 312 KEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDVSPNIPL 371

Query: 375 EKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLLEPIFPFDEKEG 434
           EKL +Y+LNVK FT  +SS+LP+++AGTF+G+ +K+ H K LG NAVLLEPIF F E++G
Sbjct: 372 EKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKG 431

Query: 435 PYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTHTAKDGALQGID 494
           PYF  HFFSP +IYGPS    SA+NSMK MVK+LH+ GIEV+LEVVFTHTA  GAL+GID
Sbjct: 432 PYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGID 491

Query: 495 DSSYFMNQVANS-EGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFINASSLLRGYDG 554
           DSSY+    AN  + K+ LNCNYP+VQQL+L+SLRYWVTEFHVDGFCFINASSLLRG  G
Sbjct: 492 DSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHG 551

Query: 555 ELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEVNSKFCNNVRDF 614
           E LSRPPLVE IAFDPLL++TK+IAD WDP E++ KE+RFPHWKRWAE+N+++C NVR+F
Sbjct: 552 EQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNF 611

Query: 615 FRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFSSGKLASELSWN 674
            RG G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN GL+LVD+VSFS  +LASELSWN
Sbjct: 612 LRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWN 671

Query: 675 CGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAYNDRRLFDW 734
           CGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     R+ FDW
Sbjct: 672 CGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDW 731

Query: 735 DLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSPPQWEDPSCKFL 794
           +LL   FGTQ TQFISF +S RARRSD+FQ R+FLK ENI WY N+Q+ P+WEDP+ KFL
Sbjct: 732 NLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFL 791

Query: 795 AVMLR--ADKEETGSSAENPKPR-GDIFMAFNASDQSESVTLPEPSEGMSWFRLIDSALS 854
           A+ ++  +++EET S AE  +P+  D+F+ FNASD  ESV LP   +G  W RL+D+AL 
Sbjct: 792 ALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALP 851

Query: 855 FPGFFSSD------PVPMTGSVTYEMQAHSCALFE 877
           FPGFFS +        P+   V YEM+ +SC LFE
Sbjct: 852 FPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Sgr019711 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 927.5 bits (2396), Expect = 7.7e-270
Identity = 460/815 (56.44%), Postives = 595/815 (73.01%), Query Frame = 0

Query: 75  SRVSIEKSGQRFGVSSKSKEHTKVATYLFRTEYGDLVNVFVRKTRSSFTVCIEISSLQPV 134
           S+ SIE         +++ +  KV +Y FRT+ G LV V V K R  +++ + +SSL+  
Sbjct: 72  SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLELS 131

Query: 135 SVDETPM-LSWGVYRSDSA-LVTSNFQSSLPDKTIGTTETPFVNTSEGKFVVELEFDAKH 194
             D++ + + WGVYRSDS+  +  +F++S  D    TTET FV +S  + ++ LEFD K 
Sbjct: 132 GDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKE 191

Query: 195 IPFYLSFLLKYRVGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGISIS-SDGSMNFSIF 254
            PFYLSF LK   G D    E+ +HR T F +PVGF  G+P PLG+S    D S NFS F
Sbjct: 192 SPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFF 251

Query: 255 SSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFSSYGYRFKGSKSH 314
           S S+ +VVLCLYDDST++KP LELDLD Y+NR+G++WHAS +   +F  YGYR K +   
Sbjct: 252 SRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHS 311

Query: 315 KNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAFDWDGEVRPNLPM 374
           K   +VE   I++DPYA ++  S+ +          K LG + + P+FDW  +V PN+P+
Sbjct: 312 KEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDVSPNIPL 371

Query: 375 EKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNLGVNAVLLEPIFPFDEKEG 434
           EKL +Y+LNVK FT  +SS+LP+++AGTF+G+ +K+ H K LG NAVLLEPIF F E++G
Sbjct: 372 EKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKG 431

Query: 435 PYFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTHTAKDGALQGID 494
           PYF  HFFSP +IYGPS    SA+NSMK MVK+LH+ GIEV+LEVVFTHTA  GAL+GID
Sbjct: 432 PYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGID 491

Query: 495 DSSYFMNQVANS-EGKNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFINASSLLRGYDG 554
           DSSY+    AN  + K+ LNCNYP+VQQL+L+SLRYWVTEFHVDGFCFINASSLLRG  G
Sbjct: 492 DSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHG 551

Query: 555 ELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEVNSKFCNNVRDF 614
           E LSRPPLVE IAFDPLL++TK+IAD WDP E++ KE+RFPHWKRWAE+N+++C NVR+F
Sbjct: 552 EQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNF 611

Query: 615 FRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVSFSSGKLASELSWN 674
            RG G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN GL+LVD+VSFS  +LASELSWN
Sbjct: 612 LRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWN 671

Query: 675 CGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAYNDRRLFDW 734
           CGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     R+ FDW
Sbjct: 672 CGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDW 731

Query: 735 DLLKRGFGTQTTQFISFFSSFRARRSDLFQSRNFLKGENIDWYGNNQSPPQWEDPSCKFL 794
           +LL   FGTQ TQFISF +S RARRSD+FQ R+FLK ENI WY N+Q+ P+WEDP+ KFL
Sbjct: 732 NLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFL 791

Query: 795 AVMLR--ADKEETGSSAENPKPR-GDIFMAFNASDQSESVTLPEPSEGMSWFRLIDSALS 854
           A+ ++  +++EET S AE  +P+  D+F+ FNASD  ESV LP   +G  W RL+D+AL 
Sbjct: 792 ALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALP 851

Query: 855 FPGFFSSD------PVPMTGSVTYEMQAHSCALFE 877
           FPGFFS +        P+   V YEM+ +SC LFE
Sbjct: 852 FPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Sgr019711 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 384.0 bits (985), Expect = 3.2e-106
Identity = 259/706 (36.69%), Postives = 371/706 (52.55%), Query Frame = 0

Query: 231 GYPSPLGISISSDGSMNFSIFSSSAESVVLCLY-----DDSTSEKPVLELDLDRYINRSG 290
           G  SPLG+S   D  +NF++FS +A SV LCL       D T +  ++EL LD  +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEG-ALNFSSYGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGH-GL 350
           + WH   E   LN   YGYR  G    +     + S +L+DPYAK++        GH   
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVK-------GHSSF 207

Query: 351 GLPSKLLGQI-----CQVPAFDWDGEVR-PNLPMEKLFIYKLNVKRFTVDKSSQLPTDIA 410
           G  S+   Q       +   FDW  + + PN+P + L IY++NV+ FT D+SS +   I 
Sbjct: 208 GDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIG 267

Query: 411 GTFAGLTKKLLHFKNLGVNAVLLEPIFPFDEKE--------------GPYFSSHFFSPTN 470
           G++ G  +K+ H ++LG+NAV L P+F FDE E                Y + +FF+P +
Sbjct: 268 GSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMS 327

Query: 471 IYGP-SGGSTSAINSMKEMVKELHANGIEVMLEVVFTHTAK-------DGALQGIDDSSY 530
            Y    G    A    KEMVK LH+ GIEV+L+VV+ HT +         + +GID+  Y
Sbjct: 328 RYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVY 387

Query: 531 FM----NQVANSEG-KNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFINASSLLRGYDG 590
           +M    NQ+ N  G  NTLNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L R  DG
Sbjct: 388 YMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDG 447

Query: 591 ELLSRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKRWAEVNSKFCNNVRDF 650
             LS PPL+  IA D +LS+ KIIA+ WD   L     +FP+W RWAE N  + ++VR F
Sbjct: 448 SPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVG-KFPNWDRWAEWNGMYRDDVRRF 507

Query: 651 FRGE-GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNFGLTLVDLVSF------SSGK 710
            +G+ G+  S ATR+ GS D++  + R P    NF+  + G TL DLVS+      ++G+
Sbjct: 508 IKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGE 567

Query: 711 LASE-----LSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS- 770
             ++      SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  
Sbjct: 568 GGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRY 627

Query: 771 GGSVAY-NDRRL--FDWDLLKRGFGTQTTQFISFFSSFRARRS-DLFQSRNFLKGENIDW 830
           G + +Y +D  L  F W  L      +   F  F    + R S  + +  NFL    I W
Sbjct: 628 GNNNSYGHDTSLNNFQWKELD---AKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITW 687

Query: 831 YGNNQSPPQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFNASDQSESVTLPEP 879
           + +N     W++   KFLA  L    +  G          DI++AFNA D      +P+P
Sbjct: 688 HEDN-----WDNSESKFLAFTLH---DGIGGR--------DIYVAFNAHDYFVKALIPQP 747

BLAST of Sgr019711 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 354.4 bits (908), Expect = 2.7e-97
Identity = 239/690 (34.64%), Postives = 347/690 (50.29%), Query Frame = 0

Query: 231 GYPSPLGISISSDGSMNFSIFSSSAESVVLCL--YDDSTSEKPVLELDLDRYINRSGNIW 290
           G PSP G ++  DG +NFS++S+++ S  +CL    D    K   E+ LD   NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 291 HASFEGALNFSSYGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSK 350
           H    G      YGYRF G  S +     + S IL+DPYAK +   I +     LG    
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAI---ISRDEFGVLGPDDN 191

Query: 351 LLGQI-CQVPA----FDWDGEVRPNLPMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGL 410
              Q+ C VP     FDW+G++   LP + L IY+++V+ FT  +SS++  +  GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251

Query: 411 TKKLLHFKNLGVNAVLLEPIFPFDEKE---------------GPYFSSHFFSPTNIYGPS 470
            +KL H K LG+N + L P   F+E E                 Y +  FFSP   Y  +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311

Query: 471 GGST---SAINSMKEMVKELHANGIEVMLEVVFTHTAKDG------ALQGIDDSSYFM-- 530
             +     AIN  K +VKE H  GIEV+++VV  HTA+        + +G+D+S Y+M  
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371

Query: 531 --NQVANSEG-KNTLNCNYPIVQQLLLDSLRYWVTEFHVDGFCFINASSLLRG------- 590
              +  N  G  NT NCN+P+V+Q +LD LRYWVTE HVDGF F   S + R        
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431

Query: 591 ------YDGELL------SRPPLVEVIAFDPLLSKTKIIADFWDPRELVSKEIRFPHWKR 650
                  +G+LL      S PP++++I+ DP+L   K+IA+ WD   L    + FPHW  
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGM-FPHWGI 491

Query: 651 WAEVNSKFCNNVRDFFRG-EGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVD 710
           W+E N KF + VR F +G +G   + A  LCGS +++  GR P  S NFI  + G TL D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551

Query: 711 LVSFSS-GKLAS----------ELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGV 770
           LV++++   LA+            SWNCGEEG  ++I V   R +Q+RNF   L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611

Query: 771 PVLNMGDECGQSSGGS----VAYNDRRLFDWDLLKRGFGTQTTQFISFFSSFRARRSDLF 830
           P++ MGDE G + GG+       N    F WD  K    +   +F      FR     L 
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRDECESL- 671

Query: 831 QSRNFLKGENIDWYGNNQSPPQWEDPSCKFLAVMLRADKEETGSSAENPKPRGDIFMAFN 850
              +F   + + W+G     P W + S +F+A  L    ++            +I++AFN
Sbjct: 672 GLNDFPTAKRLQWHGLAPEIPNWSETS-RFVAFSLVDSVKK------------EIYVAFN 731

BLAST of Sgr019711 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 68.9 bits (167), Expect = 2.3e-11
Identity = 97/416 (23.32%), Postives = 158/416 (37.98%), Query Frame = 0

Query: 192 HIPFYLSF----------LLKYRVGVDSGSSEIRSHRKTSFSVPVGFGR--GYPSPLGIS 251
           HI  Y SF          L+K ++ V    +E R  + T   +P        Y  PLG  
Sbjct: 152 HIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEGRLIQGTGLQLPGVLDELFSYDGPLGAH 211

Query: 252 ISSDGSMNFSIFSSSAESVVLCLYDDSTSEKPVLELDLDRYINRSGNIWHASFEGALNFS 311
            + +G ++  +++ +A++V +C+Y +   + P+    L      +  +W  S EGA ++ 
Sbjct: 212 FTPEG-VSLHLWAPTAQAVSVCIYKNPLDKSPMEICPL----KEANGVW--STEGACSWG 271

Query: 312 SYGYRFKGSKSHKNQDEVEVSRILVDPYAKILSTSIPKTHGHGLGLPSKLLGQICQVPAF 371
              Y +K S  H +  ++E      DPYA+ LS    KT    L            +   
Sbjct: 272 GCYYVYKVSVYHPSTMKLETC-YANDPYARGLSADGRKTFLVNLDSD--------DLKPE 331

Query: 372 DWD--GEVRPNL-PMEKLFIYKLNVKRFTVDKSSQLPTDIAGTFAGLTKKLLHFKNL--- 431
            WD   + +P L     + IY+L+V+ F+ +  +  P +  G  A  +K     K+L   
Sbjct: 332 GWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKDSAGVKHLQKL 391

Query: 432 ---GVNAVLLEPIFPF----DEKEG----------------------------------P 491
              G+  + L P F F    DEKE                                    
Sbjct: 392 VDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWG 451

Query: 492 YFSSHFFSPTNIYGPSGGSTSAINSMKEMVKELHANGIEVMLEVVFTHT------AKDGA 539
           Y    +  P   Y         I   ++MV+ L+  G+ V+L+VV+ H        K+  
Sbjct: 452 YNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHLHASGPHDKESV 511