Sgr019544 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr019544 vs. NCBI nr
Match: ALJ53304.1 (polyphenol oxidase [Luffa aegyptiaca] >APO14263.1 polyphenol oxidase 4 [Luffa aegyptiaca])

HSP 1 Score: 721.1 bits (1860), Expect = 7.5e-204
Identity = 380/592 (64.19%), Postives = 431/592 (72.80%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDR 60
           MASLSSLLSL     SKNQSSS  NR  G++Y+PCRASNG Q P+NSN +  DS+ KFDR
Sbjct: 1   MASLSSLLSLFPCIRSKNQSSSRGNRRLGLSYIPCRASNGVQTPMNSNPQEGDSMSKFDR 60

Query: 61  RDVLFGLGGLYGVATLGGDRSALAYPQSPDTANCRPATIPGGQ-ANCCPPKIHKIIDFVP 120
           RDVLFGLGGLY  A LG DRSALA P SPD  +C P T+   +  +CCPP I  I DFVP
Sbjct: 61  RDVLFGLGGLYSAAALGRDRSALADPISPDVIHCEPITLEADEKVDCCPPSILDITDFVP 120

Query: 121 PVIPKLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQLK 180
           P +  LRVRPAAHLIT +QLAK E+A+ KMK LD DDPRNFYQQA VHCAYC+ AYKQ  
Sbjct: 121 PAVENLRVRPAAHLITQEQLAKLEEAVTKMKALDPDDPRNFYQQARVHCAYCNDAYKQDG 180

Query: 181 FPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYNN- 240
           FP+ DF++HWN LFFPFHRCYLYF ERILG LINDP+FA+PFWNWDAIPGMQMP IY N 
Sbjct: 181 FPELDFDIHWNWLFFPFHRCYLYFHERILGSLINDPDFAIPFWNWDAIPGMQMPAIYIND 240

Query: 241 -PNSPLYDKLRDARHKPPKLLYLNYDG-TEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLG 300
             NSPLYD+LRD  HKPPKLL LNYDG TEDP +DV+ CN R MY+QM +  K+  GF G
Sbjct: 241 DKNSPLYDELRDPNHKPPKLLDLNYDGATEDPSYDVLFCNPRLMYRQMVINSKTAEGFHG 300

Query: 301 KLLRGGEEASYDGMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           K LR G+EA   GMGSIE S HNN+H W GDP  KY EDMG FY+A RDPLFY HH NVD
Sbjct: 301 KPLRAGQEAYRTGMGSIELSAHNNVHTWSGDPQQKYREDMGHFYAAARDPLFYAHHANVD 360

Query: 361 RMWSIWQGLEPG-PRDYTDP----------------------DC---KKLGYVYQDVEIP 420
           RMW+IWQGL  G  RD+TDP                      DC   +KLGY YQ VEIP
Sbjct: 361 RMWTIWQGLIDGKSRDFTDPDWLNASFVFYDENKNAIRIKVQDCLDNEKLGYKYQAVEIP 420

Query: 421 WLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRS 480
           WLHTK IPRQKT        S+  SS A+     + PP+    +VL+S +   V+RP RS
Sbjct: 421 WLHTKPIPRQKT--------SQLKSSKAQ-----ETPPI----EVLNSKMTKEVKRPQRS 480

Query: 481 RTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHK 540
           R+  QKS +DEVLVIE +E D+TSRVHFNVLVNVLD+G DV PGN EFAGSFK+IP GHK
Sbjct: 481 RSKTQKSVEDEVLVIE-MELDTTSRVHFNVLVNVLDDGTDVSPGNTEFAGSFKSIPLGHK 540

Query: 541 NHAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQPIN 563
           + A  SL LVITEL+EELG DDDET+ V I PK+GG+ VTIGGIKIELQPIN
Sbjct: 541 HRANTSLSLVITELIEELGYDDDETVFVTIDPKLGGDLVTIGGIKIELQPIN 574

BLAST of Sgr019544 vs. NCBI nr
Match: KAG6573251.1 (hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 700.3 bits (1806), Expect = 1.4e-197
Identity = 355/590 (60.17%), Postives = 421/590 (71.36%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDR 60
           MAS+S LLS+  YS  KNQ+S+ +N+S G+N+VPCRASNG            DS  KFDR
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNG------------DSTSKFDR 60

Query: 61  RDVLFGLGGLYGVATLGGDRS-ALAYPQSPDTANCRPATIPGGQ-ANCCPPKIHKIIDFV 120
           RDVLFGLGGLYG A L   +S A A P +PD  NC PATI G Q   CCPPKIH IIDF+
Sbjct: 61  RDVLFGLGGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHHIIDFI 120

Query: 121 -PPVIPKLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQ 180
            PP   KLR+RP AH +TP Q   FEKAI  MKRL  +DPRNFY+QA+VHCAYCDYAYKQ
Sbjct: 121 HPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQ 180

Query: 181 LKFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYN 240
           L FP A+ +VHWNALFFPFHR YLYFFERILGHLIN+P F +PFWNWD   GMQMPEIY+
Sbjct: 181 LGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYD 240

Query: 241 NPNSPLYDKLRDARHKPPKLLYLNYDGTEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLGK 300
           +PNSPLYDKLR+ RH+P KLL LNY G ++P +DVVD NLRWM+KQM     +  GFLGK
Sbjct: 241 HPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMIKDADTAEGFLGK 300

Query: 301 LLRGGEEASYD-GMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           LL   +EA+ + GMG+IE SPHNNIH+WVGDPN+K H +MG FYSA RDPLFYGHH NVD
Sbjct: 301 LLVASDEANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVD 360

Query: 361 RMWSIWQGLEPG-PRDYTDP-------------------------DCKKLGYVYQDVEIP 420
           RMWSIWQGL+ G PRD+ +P                         D ++LGYVYQ V++P
Sbjct: 361 RMWSIWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLP 420

Query: 421 WLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRS 480
           WLHTKQ PR K Q K                   QAP VI FPQ L+S + T V+RP + 
Sbjct: 421 WLHTKQTPRLKKQLK-----------------TTQAPQVIKFPQGLNSMVTTTVKRPNKL 480

Query: 481 RTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHK 540
              K+KSE DEVLVIE IE D    VHFNVLVNV+D+G DVCPGN EF G+F  +PHGH 
Sbjct: 481 MNEKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHN 540

Query: 541 NHAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQP 561
           +  K SLR+VITE+++ELG++DDE++ VR+ PK+GG++VTIGG+KIELQP
Sbjct: 541 HRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of Sgr019544 vs. NCBI nr
Match: XP_022994369.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 698.7 bits (1802), Expect = 4.0e-197
Identity = 354/590 (60.00%), Postives = 423/590 (71.69%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDR 60
           MAS+S LLS+  YS  KNQ+S+ +N+S G+N+VPCRASNG            DS  KFDR
Sbjct: 1   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNG------------DSTSKFDR 60

Query: 61  RDVLFGLGGLYGVATLGGDRS-ALAYPQSPDTANCRPATIPGGQ-ANCCPPKIHKIIDFV 120
           RDVLFGLGGLYG A L   +S A A P +PD  NC PATI G +   CCPPKIH IIDF+
Sbjct: 61  RDVLFGLGGLYGAAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFI 120

Query: 121 -PPVIPKLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQ 180
            PP   KLR+R  AH +TP Q   FEKAI  MKRL  +DPRNFY+QA+VHCAYCDYAYKQ
Sbjct: 121 HPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQ 180

Query: 181 LKFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYN 240
           L FP A+ +VHWNALFFPFHR YLYFFERILGHLIN+P+FA+PFWNWD   GMQMPEIY+
Sbjct: 181 LGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYD 240

Query: 241 NPNSPLYDKLRDARHKPPKLLYLNYDGTEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLGK 300
           +PNSPLYDKLR+ RH+P KLL LNY G ++PP+DVVDCNLRWM+KQM     +  GF GK
Sbjct: 241 HPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGK 300

Query: 301 LLRGGEEASYD-GMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           LL   +EA+ + GMG+IE SPHNNIH+WVGDPN+K + +MG FYSA RDPLFYGHH NVD
Sbjct: 301 LLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVD 360

Query: 361 RMWSIWQGLEPG-PRDYTDP-------------------------DCKKLGYVYQDVEIP 420
           RMWSIWQGL+ G PRD+ +P                         D ++LGYVYQ V++P
Sbjct: 361 RMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLP 420

Query: 421 WLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRS 480
           WLHTKQ PR K Q K                   QAP VI FPQ L+S + T V+RP + 
Sbjct: 421 WLHTKQTPRLKKQLK-----------------TTQAPQVIKFPQGLNSMVTTTVKRPKKL 480

Query: 481 RTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHK 540
           R  K+KSE DEVLVIE IE D    VHFNVLVNV+D+G DVCPGN EF G+F  + HGH 
Sbjct: 481 RDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHN 540

Query: 541 NHAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQP 561
           +  K SLR+VITE+++ELG++DDE++ VR+ PK+GG++VTIGG+KIELQP
Sbjct: 541 HRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of Sgr019544 vs. NCBI nr
Match: KAG7012418.1 (hypothetical protein SDJN02_25170, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 695.3 bits (1793), Expect = 4.4e-196
Identity = 353/590 (59.83%), Postives = 418/590 (70.85%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDR 60
           MAS+S LLS+  YS  KNQ+S+ +N+S G+N+VPCRASNG            DS  KFDR
Sbjct: 6   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNG------------DSTSKFDR 65

Query: 61  RDVLFGLGGLYGVATLGGDRS-ALAYPQSPDTANCRPATIPGGQ-ANCCPPKIHKIIDFV 120
           RDVLFGLGGLYG A L   +S A A P +PD  NC PATI G Q   CCPPKIH IIDF+
Sbjct: 66  RDVLFGLGGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFI 125

Query: 121 -PPVIPKLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQ 180
            PP   KLR+RP AH +TP Q   FEKAI  MKRL  +DPRNFY+QA+VHCAYCDYAYKQ
Sbjct: 126 HPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQ 185

Query: 181 LKFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYN 240
           L FP A+ +VHWNALFFPFHR YLYFFERILGHLIN+P F +PFWNWD   GMQMPEIY+
Sbjct: 186 LGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYD 245

Query: 241 NPNSPLYDKLRDARHKPPKLLYLNYDGTEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLGK 300
           +PNSPLYDKLR+ RH+P KLL LNY G ++P +DVVD NLRWM+KQM     +  GF GK
Sbjct: 246 HPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGK 305

Query: 301 LLRGGEEASYD-GMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           LL    EA+ + GMG+IE SPHNNIH+WVGDPN+K H +MG FYSA RDPLFYGHH NVD
Sbjct: 306 LLIASHEANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVD 365

Query: 361 RMWSIWQGLEPG-PRDYTDP-------------------------DCKKLGYVYQDVEIP 420
           RMWSIWQGL+ G PRD+ +P                         D ++LGYVYQ V++P
Sbjct: 366 RMWSIWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLP 425

Query: 421 WLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRS 480
           WLHTKQ PR K Q K                   QAP VI FPQ L+S + T V+RP + 
Sbjct: 426 WLHTKQTPRLKKQLK-----------------TTQAPQVIKFPQGLNSMVTTTVKRPNKL 485

Query: 481 RTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHK 540
              K+KSE DEVLVIE IE D    VHFNVLVNV+D+G DVCPGN EF G+F  +PHGH 
Sbjct: 486 MNEKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHN 545

Query: 541 NHAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQP 561
           +  K SLR+VITE+++ELG++DDE++ VR+ PK+GG++VTIGG+KIE QP
Sbjct: 546 HRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIEHQP 566

BLAST of Sgr019544 vs. NCBI nr
Match: XP_022954613.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 689.9 bits (1779), Expect = 1.9e-194
Identity = 351/590 (59.49%), Postives = 416/590 (70.51%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDR 60
           MAS+S LLS+  YS  KNQ+S+ +N+S G+N VPCRASNG            DS  KFDR
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINQVPCRASNG------------DSTSKFDR 60

Query: 61  RDVLFGLGGLYGVATLGGDRS-ALAYPQSPDTANCRPATIPGGQ-ANCCPPKIHKIIDFV 120
           RDVLFGLGGLYG A L   +S A A P +PD  NC  ATI G +   CCPPKIH IIDF+
Sbjct: 61  RDVLFGLGGLYGAAALTNQQSPASAAPLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFI 120

Query: 121 -PPVIPKLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQ 180
            PP   KLR+RP AH +TP Q   FEKAI  MKRL  +DPRNFY+QA+VHCAYCDYAYKQ
Sbjct: 121 HPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQ 180

Query: 181 LKFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYN 240
           L FP A+ +VHWNALFFPFHR YLYFFERILGHLIN+P F +PFWNWD   GMQMPEIY+
Sbjct: 181 LGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYD 240

Query: 241 NPNSPLYDKLRDARHKPPKLLYLNYDGTEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLGK 300
           +PNSPLYDKLR+ RH+P KLL LNY G ++P +DVVD NLRWM+KQM     +  GF GK
Sbjct: 241 HPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGK 300

Query: 301 LLRGGEEASYD-GMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           LL    EA+ + GMG+IE SPHNNIH+WVGDPN+K H +MG FYSA RDPLFYGHH NVD
Sbjct: 301 LLIASHEANEETGMGTIEKSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVD 360

Query: 361 RMWSIWQGLEPG-PRDYTDP-------------------------DCKKLGYVYQDVEIP 420
           RMWSIWQGL+ G  RD+ +P                         D ++LGYVYQ V++P
Sbjct: 361 RMWSIWQGLKNGKARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLP 420

Query: 421 WLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRS 480
           WLHTKQ PR K Q K                   QAP VI FPQ L+S + T V+RP + 
Sbjct: 421 WLHTKQTPRLKKQLK-----------------TTQAPQVIKFPQGLNSMVTTTVKRPNKL 480

Query: 481 RTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHK 540
              K+KSE DEVLVIE IE D    VHFNVLVNV+D+G DVCPGN EF G+F  +PHGH 
Sbjct: 481 MNEKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHN 540

Query: 541 NHAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQP 561
           +  K SLR+VITE+++ELG++DDE++ VR+ PK+GG++VTIGG+KIELQP
Sbjct: 541 HRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of Sgr019544 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.8e-134
Identity = 270/559 (48.30%), Postives = 343/559 (61.36%), Query Frame = 0

Query: 33  VPCRASNGDQAPVNSNARGDDSVIKFDRRDVLFGLGGLYGVATLGGDRSALAYP-QSPDT 92
           V C+A+N DQ         D +  K DRR+VL GLGGLYGVA +G D  A A P   PD 
Sbjct: 46  VSCKATNNDQ--------NDQAQSKLDRRNVLLGLGGLYGVAGMGTDPFAFAKPIAPPDV 105

Query: 93  ANCRPATIPGG--QANCCPPKIHKIIDFVPPVIPKLRVRPAAHLITPDQLAKFEKAIDKM 152
           + C PA +P G    NCCPP   KIIDF  P   KLR+RP AH +      K+ KA++ M
Sbjct: 106 SKCGPADLPQGAVPTNCCPPPSTKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAMELM 165

Query: 153 KRLDKDDPRNFYQQANVHCAYCDYAYKQLKFPKADFEVHWNALFFPFHRCYLYFFERILG 212
           K L  DDPR+F QQA VHCAYCD AY Q+ FP+ + ++H + LFFPFHR YLYFFE+ILG
Sbjct: 166 KALPDDDPRSFKQQAAVHCAYCDGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEKILG 225

Query: 213 HLINDPEFAMPFWNWDAIPGMQMPEIYNNPNSPLYDKLRDARHKPPKLLYLNYDGTED-- 272
            LINDP FA+PFWNWD+  GM +P IY +P SPLYDKLR A H+PP L+ L+Y+GTED  
Sbjct: 226 KLINDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNV 285

Query: 273 PPFDVVDCNLRWMYKQMKVTGKSTRGFLGKLLRGGEEASYDGMGSIESSPHNNIHDWVGD 332
                ++ NL+ MY+QM    K+ + F G   R G+E    G GSIE +PH  +H W GD
Sbjct: 286 SKETTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPD-PGGGSIEGTPHAPVHLWTGD 345

Query: 333 PNSKYHEDMGTFYSAGRDPLFYGHHGNVDRMWSIWQGLEPGPRDYTDPD----------- 392
                 EDMG FYSAGRDP+F+ HH NVDRMWSIW+ L     D TD D           
Sbjct: 346 NTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNE 405

Query: 393 --------------CKKLGYVYQDVEIPWLHTKQIPRQ-KTQFKKITKESEPSSSSAEPS 452
                          K LGYVYQDV+IPWL +K  PR+ K    K+ K+   + ++   S
Sbjct: 406 NAELVRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASS 465

Query: 453 SEPQAPPVISFPQVLDSNINTVVQRP-YRSRTIKQKSEDDEVLVIEDIEFDSTSRVHFNV 512
           S+  A     FP  L S I+TVV+RP  + R+ K K +++E+LVIE IEFD    V F+V
Sbjct: 466 SKVVAG--TEFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDV 525

Query: 513 LVNVLDEGLDVCPGNLEFAGSFKNIPHGHKNHAKAS--LRLVITELVEELGLDDDETLSV 558
            VN +D+ L   P   EFAGSF ++PH HK+  K +  LRL +T+L+EE+  +DD+++ V
Sbjct: 526 YVNDVDD-LPSGPDKTEFAGSFVSVPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVV 585

BLAST of Sgr019544 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 2.6e-122
Identity = 258/565 (45.66%), Postives = 336/565 (59.47%), Query Frame = 0

Query: 33  VPCRASNGDQAPVNSNARGDDSVIKFDRRDVLFGLGGLYGVATLGGDRSALAYPQS-PDT 92
           V C ++N    P       + S I   RR+VL GLGG+YG  TL  + SALA P S PD 
Sbjct: 46  VTCSSNNNQNNPKEEQ---ELSNIVGHRRNVLIGLGGIYG--TLATNPSALASPISPPDL 105

Query: 93  ANC-RPATIPGG----QANCCPPKIHKIIDFVPPVIPKLRVRPAAHLITPDQLAKFEKAI 152
           + C  P+ +P G      NCCPP   KI DF  P    LRVR AAHL+  + L K++KA 
Sbjct: 106 SKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSNQPLRVRQAAHLVDNEFLEKYKKAT 165

Query: 153 DKMKRLDKDDPRNFYQQANVHCAYCDYAYKQLKFPKADFEVHWNALFFPFHRCYLYFFER 212
           + MK L  +DPRNF QQAN+HCAYCD AY Q+ FP    +VH + LFFPFHR YLYF+ER
Sbjct: 166 ELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGFPDLKLQVHGSWLFFPFHRWYLYFYER 225

Query: 213 ILGHLINDPEFAMPFWNWDAIPGMQMPEIYNNPNSPLYDKLRDARHKPPKLLYLNY--DG 272
           ILG LINDP FA+PFWN+DA  GMQ+P IY +  SPLYD+LR+A H+PP L+ LN+   G
Sbjct: 226 ILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDELRNASHQPPTLIDLNFCDIG 285

Query: 273 TEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLGKLLRGGEEASYDGMGSIESSPHNNIHDW 332
           ++    +++  NL  MY+Q+   GK++R FLG   R G +A   G GSIE+ PH  +H W
Sbjct: 286 SDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRAG-DAEPQGAGSIENVPHAPVHTW 345

Query: 333 VGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVDRMWSIWQGLEPGPRDYTDP--------- 392
            GD      EDMG FYSA RDP+FY HH NVDR+W IW+ L     D+TD          
Sbjct: 346 TGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYIWKTLGGKKHDFTDKDWLESGFLF 405

Query: 393 ----------------DCKKLGYVYQDVEIPWLHTKQIPRQKTQFKKITKESEPSSSSAE 452
                           D  KLGY YQDV IPW   K +PR+    K +  E    +    
Sbjct: 406 YDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKAKPVPRRTKVQKLVEVEVNDGNLRKS 465

Query: 453 PS---SEPQAP-PVISFPQVLDSNINTVVQRPYRSRTIKQKSEDDEVLVIEDIEFDSTSR 512
           P+      Q+P   ++FP VL++ ++ +V+RP + R+ K+K E++EVLVIE IEF     
Sbjct: 466 PTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIA 525

Query: 513 VHFNVLVNVLDEGLDVCPGNLEFAGSFKNIP---HGHKN-HAKASLRLVITELVEELGLD 557
           + F+V +N  DE   V  GN EFAGSF NIP   HGHKN     SLRL IT+L+E+L ++
Sbjct: 526 IKFDVYIN--DEDDKVGAGNTEFAGSFVNIPHSAHGHKNKKIITSLRLGITDLLEDLHVE 585

BLAST of Sgr019544 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 5.3e-120
Identity = 252/571 (44.13%), Postives = 338/571 (59.19%), Query Frame = 0

Query: 24  RNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDRRDVLFGLGGLYGVATLGGDRSAL 83
           R+R    + V C ++NGD    +++   + S  K DRR+VL G+GGLYG A   G    L
Sbjct: 41  RSRRFAPSKVSCNSANGDPNSDSTSDVRETSSGKLDRRNVLLGIGGLYGAAGGLGATKPL 100

Query: 84  AYP---QSPDTANCRPATIPGG--QANCCPPKIHKIIDF-VPPVIPKLRVRPAAHLITPD 143
           A+    Q+PD + C  AT+P G    NCCPP   KIIDF +P     +R RPAAHL++ +
Sbjct: 101 AFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLPSSGSPMRTRPAAHLVSKE 160

Query: 144 QLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQLKFPKADFEVHWNALFFPFH 203
            LAK++KAI+  K L  DDPR+F QQANVHC YC  AY Q+ +   + +VH + LF PFH
Sbjct: 161 YLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVGYTDLELQVHASWLFLPFH 220

Query: 204 RCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYNNPNSPLYDKLRDARHKPPKL 263
           R YLYF ERIL  LI+DP FA+P+W WD   GM MP IY +  S LYD+ R+A+H PP +
Sbjct: 221 RYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSPSSLYDEKRNAKHLPPTV 280

Query: 264 LYLNYDGTED--PPFDVVDCNLRWMYKQMKVTGKSTRGFLGKLLRGGEEASYDGMGSIES 323
           + L+YDGTE   P  ++   NL  MYKQ+     + + FLG   R G +A   G G++E 
Sbjct: 281 IDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLGYPYRAG-DAIDPGAGTLEH 340

Query: 324 SPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVDRMWSIWQGL-EPGPRDYTD 383
           +PHN +H W G  + K  EDMG FY+AGRDP+F+GHH NVDRMW+IW+ +     +D+TD
Sbjct: 341 APHNIVHKWTGLAD-KPSEDMGNFYTAGRDPIFFGHHANVDRMWNIWKTIGGKNRKDFTD 400

Query: 384 P-------------------------DCKKLGYVYQDVEIPWLHTKQIPRQKTQFKKITK 443
                                     D  KL Y YQD+ IPWL        K + K  TK
Sbjct: 401 TDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLP----KNTKAKAKTTTK 460

Query: 444 ESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRSRTIKQKSEDDEVLVIEDIE 503
            S+   + A    +     +  FP+ L+S I   V RP +SR+ K+K +++EVL+I+ IE
Sbjct: 461 SSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSKKEKEDEEEVLLIKGIE 520

Query: 504 FDSTSRVHFNVLVNVLDEGLDVC-PGNLEFAGSFKNIPHGHKNH--AKASLRLVITELVE 558
            D  + V F+V +N  DE   V  P N EFAGSF N+PH H      K +LR  I EL+E
Sbjct: 521 LDRENFVKFDVYIN--DEDYSVSRPKNSEFAGSFVNVPHKHMKEMKTKTNLRFAINELLE 580

BLAST of Sgr019544 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-118
Identity = 258/601 (42.93%), Postives = 345/601 (57.40%), Query Frame = 0

Query: 1   MASLSSLLSLPQYS---GSKNQSSSPRNRSHGV---NYVPCRASNGDQAPVNSNARGDDS 60
           M + SSL+S   +S    +K+Q S      H V   + + C+A+N + +  N N + ++S
Sbjct: 10  MGTYSSLISTNSFSTFLPNKSQLSLSGKSKHYVARRSSISCKATNNNNSN-NQNEQQEES 69

Query: 61  ---VIKFDRRDVLFGLGGLYGVATLGGDRSALAYP-QSPDTANCRPATI-PGGQAN--CC 120
              + K DRR++L GLGGLYG  TL     A A P   PD   C+PA I PGG     CC
Sbjct: 70  SRLLGKLDRRNILIGLGGLYGATTLDRKPFAFADPIAPPDLTTCKPAEITPGGSETVPCC 129

Query: 121 PPKIHKIIDFVPPVIPKLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVH 180
           PP   KI  F P +   LR  PAAH +T + LAKF+KA   M+ L  DDPR+  QQA VH
Sbjct: 130 PPVTTKIKTFKPDLSIPLRTSPAAHQVTDEYLAKFKKAQAAMRALPDDDPRSMVQQAKVH 189

Query: 181 CAYCDYAYKQLKFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAI 240
           CAYC+ AY Q+ F   D +VH++ LFFPFHR YLYF+ERILG LI+DP FA+P+WNWD+ 
Sbjct: 190 CAYCNGAYPQVGFTDNDIQVHFSWLFFPFHRMYLYFYERILGKLIDDPTFALPYWNWDSP 249

Query: 241 PGMQMPEIYNNPNSPLYDKLRDARHKPPKLLYLNYDGTEDPPFDV--VDCNLRWMYKQMK 300
            G  +P+IY + +SPLYD+ R+A H+PP L+ L+Y G +D   +   +D NL  MY+QM 
Sbjct: 250 VGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDLSYGGKDDDVDEQTRIDENLAIMYRQMV 309

Query: 301 VTGKSTRGFLGKLLRGGEEASYDGMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRD 360
              K+   F G   R G   +    G+IE+ PHNNIH WVGDP+  + EDMG FYSAGRD
Sbjct: 310 SGAKTPDLFFGHAYRAGNLNTGKYPGTIENMPHNNIHIWVGDPSQTHQEDMGNFYSAGRD 369

Query: 361 PLFYGHHGNVDRMWSIWQGLEPGPRDYTDPDC--------------------------KK 420
           PLFY HH NVDRMW+IW+ L    +D TD D                           K+
Sbjct: 370 PLFYAHHANVDRMWNIWKTLGGKRKDITDTDWLDAEFLFYDENAELVRVKVRDSLEPEKQ 429

Query: 421 LGYVYQDVEIPWLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSN 480
           L Y Y+ V +PWL TK   R KT+ K   K +    +S              FP  L   
Sbjct: 430 LRYNYEPVSLPWLFTKPTAR-KTKNKTKAKVAATQLTS-------------KFPATLVEV 489

Query: 481 INTVVQRPY-RSRTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEF 540
               V RP  R R+ K+K +++E+L+I+DIEF+ T  V F+V +N   E L       EF
Sbjct: 490 TTVEVARPKPRKRSKKEKVDEEELLIIKDIEFEGTEAVKFDVFINDDAESLS-RRDKSEF 549

Query: 541 AGSFKNIPHGH--KNHAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKI 558
           AGSF ++P G   K   K +L+L IT+L+E+LG +DD ++ V +VP++    +TIGG KI
Sbjct: 550 AGSFVHVPQGKTTKAKTKTNLKLGITDLLEDLGAEDDSSVLVTLVPRVSNSPITIGGFKI 594

BLAST of Sgr019544 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.8e-115
Identity = 245/596 (41.11%), Postives = 338/596 (56.71%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDR 60
           MASLSS   L     S    + P   S  +  V C+ASN ++   N      +     DR
Sbjct: 1   MASLSSQSKLLATPYSFPYHTKPSRVS--LRRVSCKASNDNKDKPND----QEKTFSIDR 60

Query: 61  RDVLFGLGGLYGVATL-GGDRSALAYPQSPDTA--NCRPA--TIPGGQANCCPPKIHKII 120
           R++L GLGGLYG + +   ++S LA P  P     +C P      G    CCPP +   I
Sbjct: 61  RNMLIGLGGLYGASNVFPSNQSTLAAPIQPPVLPDSCHPPEDLAEGVNVLCCPPDVKDPI 120

Query: 121 DFVPPVIP-KLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYA 180
           DF  P  P +LR+RPAAHL  P  + K++KA+  MK L ++DPR+FYQQAN+HCAYC+ A
Sbjct: 121 DFQMPSNPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHCAYCNGA 180

Query: 181 YKQLKFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPE 240
           Y Q+ FP  + +VH + LF PFHR YLYF+ERILG LI+DP FA+PFWNWDA  GM MP 
Sbjct: 181 YDQVGFPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMHMPH 240

Query: 241 IYNNPNSPLYDKLRDARHKPPKLLYLNYDGTEDPPFD--VVDCNLRWMYKQMKVTGKSTR 300
           ++ +PNSPLYD  R+  H P  ++ L++   E P  +   +  NL  MYKQ+ V  K  R
Sbjct: 241 MFIDPNSPLYDAKRNPAHFPDTIVDLDFSSGEAPSHNPRQIGNNLSIMYKQV-VRAKKAR 300

Query: 301 GFLGKLLRGGEEASYDGMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHH 360
            F G+ L+ G      G GS+E +PH NIH W GDP     E+MG FYSAGRDPLFY HH
Sbjct: 301 LFHGRPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPRQSNFENMGNFYSAGRDPLFYAHH 360

Query: 361 GNVDRMWSIWQGLEPGPRDYTDPDC-------------------------KKLGYVYQDV 420
            NVDRMW IW+    G +DY   D                          +KLGY YQ+V
Sbjct: 361 ANVDRMWYIWKN-SLGRKDYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLGYAYQEV 420

Query: 421 EIPWLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRP 480
           +IPW+++K  PR          ++ P  +     +      +  FP  LDS ++  V+RP
Sbjct: 421 DIPWINSKPKPR----------KANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRP 480

Query: 481 YRSRTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPH 540
            +SR+  +K +++EVLVIE I+ +    + F+V +N  DE         EFAGSF N+PH
Sbjct: 481 KKSRSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYINDEDEDPSAA-DQTEFAGSFVNVPH 540

Query: 541 GHKNHAK------ASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIE 558
            H++  K       +L + I+E+++EL +D D+++ V +VP++G  ++TIGG KIE
Sbjct: 541 FHRHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIE 577

BLAST of Sgr019544 vs. ExPASy TrEMBL
Match: A0A172DC01 (Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 3.6e-204
Identity = 380/592 (64.19%), Postives = 431/592 (72.80%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDR 60
           MASLSSLLSL     SKNQSSS  NR  G++Y+PCRASNG Q P+NSN +  DS+ KFDR
Sbjct: 1   MASLSSLLSLFPCIRSKNQSSSRGNRRLGLSYIPCRASNGVQTPMNSNPQEGDSMSKFDR 60

Query: 61  RDVLFGLGGLYGVATLGGDRSALAYPQSPDTANCRPATIPGGQ-ANCCPPKIHKIIDFVP 120
           RDVLFGLGGLY  A LG DRSALA P SPD  +C P T+   +  +CCPP I  I DFVP
Sbjct: 61  RDVLFGLGGLYSAAALGRDRSALADPISPDVIHCEPITLEADEKVDCCPPSILDITDFVP 120

Query: 121 PVIPKLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQLK 180
           P +  LRVRPAAHLIT +QLAK E+A+ KMK LD DDPRNFYQQA VHCAYC+ AYKQ  
Sbjct: 121 PAVENLRVRPAAHLITQEQLAKLEEAVTKMKALDPDDPRNFYQQARVHCAYCNDAYKQDG 180

Query: 181 FPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYNN- 240
           FP+ DF++HWN LFFPFHRCYLYF ERILG LINDP+FA+PFWNWDAIPGMQMP IY N 
Sbjct: 181 FPELDFDIHWNWLFFPFHRCYLYFHERILGSLINDPDFAIPFWNWDAIPGMQMPAIYIND 240

Query: 241 -PNSPLYDKLRDARHKPPKLLYLNYDG-TEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLG 300
             NSPLYD+LRD  HKPPKLL LNYDG TEDP +DV+ CN R MY+QM +  K+  GF G
Sbjct: 241 DKNSPLYDELRDPNHKPPKLLDLNYDGATEDPSYDVLFCNPRLMYRQMVINSKTAEGFHG 300

Query: 301 KLLRGGEEASYDGMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           K LR G+EA   GMGSIE S HNN+H W GDP  KY EDMG FY+A RDPLFY HH NVD
Sbjct: 301 KPLRAGQEAYRTGMGSIELSAHNNVHTWSGDPQQKYREDMGHFYAAARDPLFYAHHANVD 360

Query: 361 RMWSIWQGLEPG-PRDYTDP----------------------DC---KKLGYVYQDVEIP 420
           RMW+IWQGL  G  RD+TDP                      DC   +KLGY YQ VEIP
Sbjct: 361 RMWTIWQGLIDGKSRDFTDPDWLNASFVFYDENKNAIRIKVQDCLDNEKLGYKYQAVEIP 420

Query: 421 WLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRS 480
           WLHTK IPRQKT        S+  SS A+     + PP+    +VL+S +   V+RP RS
Sbjct: 421 WLHTKPIPRQKT--------SQLKSSKAQ-----ETPPI----EVLNSKMTKEVKRPQRS 480

Query: 481 RTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHK 540
           R+  QKS +DEVLVIE +E D+TSRVHFNVLVNVLD+G DV PGN EFAGSFK+IP GHK
Sbjct: 481 RSKTQKSVEDEVLVIE-MELDTTSRVHFNVLVNVLDDGTDVSPGNTEFAGSFKSIPLGHK 540

Query: 541 NHAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQPIN 563
           + A  SL LVITEL+EELG DDDET+ V I PK+GG+ VTIGGIKIELQPIN
Sbjct: 541 HRANTSLSLVITELIEELGYDDDETVFVTIDPKLGGDLVTIGGIKIELQPIN 574

BLAST of Sgr019544 vs. ExPASy TrEMBL
Match: A0A6J1K2N9 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111490108 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.9e-197
Identity = 354/590 (60.00%), Postives = 423/590 (71.69%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDR 60
           MAS+S LLS+  YS  KNQ+S+ +N+S G+N+VPCRASNG            DS  KFDR
Sbjct: 1   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNG------------DSTSKFDR 60

Query: 61  RDVLFGLGGLYGVATLGGDRS-ALAYPQSPDTANCRPATIPGGQ-ANCCPPKIHKIIDFV 120
           RDVLFGLGGLYG A L   +S A A P +PD  NC PATI G +   CCPPKIH IIDF+
Sbjct: 61  RDVLFGLGGLYGAAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFI 120

Query: 121 -PPVIPKLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQ 180
            PP   KLR+R  AH +TP Q   FEKAI  MKRL  +DPRNFY+QA+VHCAYCDYAYKQ
Sbjct: 121 HPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQ 180

Query: 181 LKFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYN 240
           L FP A+ +VHWNALFFPFHR YLYFFERILGHLIN+P+FA+PFWNWD   GMQMPEIY+
Sbjct: 181 LGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYD 240

Query: 241 NPNSPLYDKLRDARHKPPKLLYLNYDGTEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLGK 300
           +PNSPLYDKLR+ RH+P KLL LNY G ++PP+DVVDCNLRWM+KQM     +  GF GK
Sbjct: 241 HPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGK 300

Query: 301 LLRGGEEASYD-GMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           LL   +EA+ + GMG+IE SPHNNIH+WVGDPN+K + +MG FYSA RDPLFYGHH NVD
Sbjct: 301 LLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVD 360

Query: 361 RMWSIWQGLEPG-PRDYTDP-------------------------DCKKLGYVYQDVEIP 420
           RMWSIWQGL+ G PRD+ +P                         D ++LGYVYQ V++P
Sbjct: 361 RMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLP 420

Query: 421 WLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRS 480
           WLHTKQ PR K Q K                   QAP VI FPQ L+S + T V+RP + 
Sbjct: 421 WLHTKQTPRLKKQLK-----------------TTQAPQVIKFPQGLNSMVTTTVKRPKKL 480

Query: 481 RTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHK 540
           R  K+KSE DEVLVIE IE D    VHFNVLVNV+D+G DVCPGN EF G+F  + HGH 
Sbjct: 481 RDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHN 540

Query: 541 NHAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQP 561
           +  K SLR+VITE+++ELG++DDE++ VR+ PK+GG++VTIGG+KIELQP
Sbjct: 541 HRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of Sgr019544 vs. ExPASy TrEMBL
Match: A0A6J1GRK5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456826 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 9.0e-195
Identity = 351/590 (59.49%), Postives = 416/590 (70.51%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSHGVNYVPCRASNGDQAPVNSNARGDDSVIKFDR 60
           MAS+S LLS+  YS  KNQ+S+ +N+S G+N VPCRASNG            DS  KFDR
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINQVPCRASNG------------DSTSKFDR 60

Query: 61  RDVLFGLGGLYGVATLGGDRS-ALAYPQSPDTANCRPATIPGGQ-ANCCPPKIHKIIDFV 120
           RDVLFGLGGLYG A L   +S A A P +PD  NC  ATI G +   CCPPKIH IIDF+
Sbjct: 61  RDVLFGLGGLYGAAALTNQQSPASAAPLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFI 120

Query: 121 -PPVIPKLRVRPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQ 180
            PP   KLR+RP AH +TP Q   FEKAI  MKRL  +DPRNFY+QA+VHCAYCDYAYKQ
Sbjct: 121 HPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQ 180

Query: 181 LKFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYN 240
           L FP A+ +VHWNALFFPFHR YLYFFERILGHLIN+P F +PFWNWD   GMQMPEIY+
Sbjct: 181 LGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYD 240

Query: 241 NPNSPLYDKLRDARHKPPKLLYLNYDGTEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLGK 300
           +PNSPLYDKLR+ RH+P KLL LNY G ++P +DVVD NLRWM+KQM     +  GF GK
Sbjct: 241 HPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGK 300

Query: 301 LLRGGEEASYD-GMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           LL    EA+ + GMG+IE SPHNNIH+WVGDPN+K H +MG FYSA RDPLFYGHH NVD
Sbjct: 301 LLIASHEANEETGMGTIEKSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVD 360

Query: 361 RMWSIWQGLEPG-PRDYTDP-------------------------DCKKLGYVYQDVEIP 420
           RMWSIWQGL+ G  RD+ +P                         D ++LGYVYQ V++P
Sbjct: 361 RMWSIWQGLKNGKARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLP 420

Query: 421 WLHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRS 480
           WLHTKQ PR K Q K                   QAP VI FPQ L+S + T V+RP + 
Sbjct: 421 WLHTKQTPRLKKQLK-----------------TTQAPQVIKFPQGLNSMVTTTVKRPNKL 480

Query: 481 RTIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHK 540
              K+KSE DEVLVIE IE D    VHFNVLVNV+D+G DVCPGN EF G+F  +PHGH 
Sbjct: 481 MNEKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHN 540

Query: 541 NHAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQP 561
           +  K SLR+VITE+++ELG++DDE++ VR+ PK+GG++VTIGG+KIELQP
Sbjct: 541 HRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of Sgr019544 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 5.5e-168
Identity = 319/590 (54.07%), Postives = 396/590 (67.12%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSH-GVNYVPCRASNGDQAPVNSNARGDDSVIKFD 60
           MASLSSLL L  YS +K + S   N+ H  VNYV C+A+        S+  G+DSV KFD
Sbjct: 1   MASLSSLLLLSPYSQTKTKFSISSNKRHVVVNYVSCKAA--------SSNNGEDSVSKFD 60

Query: 61  RRDVLFGLGGLYGVATLGGDRSALAYPQSPDTANCRPATIPGGQANCCPPKIHKIIDFVP 120
           RR+VLFGLGGLYGV T  G  S LA P +PD  NC  A  PGG  NCCPP+I KII FVP
Sbjct: 61  RREVLFGLGGLYGVVTATG--SVLAAPLTPDVFNCDMAQTPGGIKNCCPPRIGKIIHFVP 120

Query: 121 PVIPKLRV-RPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQL 180
           P I +  V RPAAHL+   Q+ + E+ I K+K+LD+DDP +F  QA +HCAYCDYAY QL
Sbjct: 121 PKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQL 180

Query: 181 -KFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYN 240
               +  FEVH NALFFPFHR YLYFFERIL H I +P+FA+PFWNWD+ PGM MPE YN
Sbjct: 181 GSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYN 240

Query: 241 NPNSPLYDKLRDARHKPPKLLYLNYDG-TEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLG 300
            P+S LYD LRD RH P KL+ LNY+G TED  FDVVDCNLRWM  +MKV  +S R FLG
Sbjct: 241 RPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKV--QSPRSFLG 300

Query: 301 KLLRGGEEASYDGMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           ++L+ GE      MG+IE SPHNNIHDWVG   + + EDMG+FYSA  DPLFY HH N+D
Sbjct: 301 RVLKAGETPKPSDMGTIEQSPHNNIHDWVG-TKTPHFEDMGSFYSAALDPLFYAHHTNID 360

Query: 361 RMWSIWQGLEPGPRDYTDP-------------------------DCKKLGYVYQDVEIPW 420
           R+W+IW+ L+  P+D+TDP                         D +KLGYVYQ+V +PW
Sbjct: 361 RLWNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPW 420

Query: 421 LHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRSR 480
           L   +IP      +               + EP+ PP+I+FPQ L+S+I T+V RP  + 
Sbjct: 421 LLLNKIPSPNPWIR---------------TEEPE-PPIINFPQTLNSDITTMVDRPEEAG 480

Query: 481 TIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHKN 540
           +     E D VLV+ED+E+D T+RVHF+VLVNV+DE  D C   LEF GSFKNIPHG  +
Sbjct: 481 S---GEEVDVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHH 540

Query: 541 HAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQPI 562
           H K SLR VITE+++ LGL+ D+ L+V+I P +GGE VT+GGIKI+L+P+
Sbjct: 541 HDKTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 558

BLAST of Sgr019544 vs. ExPASy TrEMBL
Match: A0A0A0LUI1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.6e-167
Identity = 315/590 (53.39%), Postives = 396/590 (67.12%), Query Frame = 0

Query: 1   MASLSSLLSLPQYSGSKNQSSSPRNRSH-GVNYVPCRASNGDQAPVNSNARGDDSVIKFD 60
           MASLSSLL L  YS +K +SS   N+ H  VNYV C+A++ +         G+DSV KFD
Sbjct: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNN---------GEDSVSKFD 60

Query: 61  RRDVLFGLGGLYGVATLGGDRSALAYPQSPDTANCRPATIPGGQANCCPPKIHKIIDFVP 120
           RR+VLFGLGGLYGV T     S LA P +PD  NC  A  PGG  +CCPP+I KII FVP
Sbjct: 61  RREVLFGLGGLYGVVT--ATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVP 120

Query: 121 PVIPKLRV-RPAAHLITPDQLAKFEKAIDKMKRLDKDDPRNFYQQANVHCAYCDYAYKQL 180
           P I +  V RPAAHL+   Q+ + E  I K+K+LD+ DP +F  QA +HCAYCDY Y QL
Sbjct: 121 PKIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQL 180

Query: 181 -KFPKADFEVHWNALFFPFHRCYLYFFERILGHLINDPEFAMPFWNWDAIPGMQMPEIYN 240
               +  F VH NALFFPFHR YLYFFERIL H I +P FA+PFWNWD+ PGM MPE YN
Sbjct: 181 GSITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYN 240

Query: 241 NPNSPLYDKLRDARHKPPKLLYLNYDG-TEDPPFDVVDCNLRWMYKQMKVTGKSTRGFLG 300
            P+S LYD LRD RH P KL+YLNY+G TED  FDVVDCNLRWM  +M+V  +S R FLG
Sbjct: 241 RPSSSLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQV--QSPRSFLG 300

Query: 301 KLLRGGEEASYDGMGSIESSPHNNIHDWVGDPNSKYHEDMGTFYSAGRDPLFYGHHGNVD 360
           ++L+ GE  +   MG+IE SPHNNIHDW+G   + + EDMG+FYSA  DPLFY HH N+D
Sbjct: 301 RVLKAGETPNPTDMGTIEQSPHNNIHDWLG-TKTPHFEDMGSFYSAALDPLFYAHHANID 360

Query: 361 RMWSIWQGLEPGPRDYTDP-------------------------DCKKLGYVYQDVEIPW 420
           R+W+IW+ L+  P+D+ DP                         D +KLGYVYQ+V +PW
Sbjct: 361 RLWNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPW 420

Query: 421 LHTKQIPRQKTQFKKITKESEPSSSSAEPSSEPQAPPVISFPQVLDSNINTVVQRPYRSR 480
           LH  +IP      +  T+ESE              PP+++FPQ+L+S+I T+V RP   R
Sbjct: 421 LHMNKIPTPNPWIR--TEESE--------------PPIVNFPQILNSDITTMVDRPEEDR 480

Query: 481 TIKQKSEDDEVLVIEDIEFDSTSRVHFNVLVNVLDEGLDVCPGNLEFAGSFKNIPHGHKN 540
           +     E DEVLV+ED+E+D T+RVHF+VLVNV+D+  D C   LEF GSFKNIPHG  +
Sbjct: 481 S---GEEVDEVLVLEDVEYDPTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHH 540

Query: 541 HAKASLRLVITELVEELGLDDDETLSVRIVPKIGGERVTIGGIKIELQPI 562
           H K SLR VITE+++ LGL+ D+ L+V+I P +GGE VT+GGIKI+L+P+
Sbjct: 541 HDKTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQLEPL 557