Sgr019437 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr019437
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	9-cis-epoxycarotenoid dioxygenase
Location	tig00153347: 604509 .. 606277 (-)
RNA-Seq Expression	Sgr019437
Synteny	Sgr019437

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCCTCCGTCTCTCCTCCTCCTCCTCCTCTGCCACCGTTGTTGCGGACTTGGGCCTGAACTTGGGCTTGGGCTTTCCCACAAGGCCCATTAACATTGGGAAGAAGCCCAATTGTTGCGCTCTAAACTCCCCGTCTGTGTTCCATTTCCCGAAACAGCCCAATAAACGACCACAAATTATGGAAGAGGACACCTCCAGGTGTCGTCAACCTCCGCACTGGAATTTTCTGCAACGAGGTGCGTCCTTCGCGCTCGACATGCTCGAACAAGCCTTGCTCTCCAATAACGACAACCGGAATCTTCCAAAAACCTTCGACCCACGTGTTCAAATCGCCGGGAACTACGCTCCGGTGCCGAACAACCAGTCTGTCACTCTCTGCCGATCGCCGGGGCCATCCCTGACAGTATCCAAGGCGTCTATCTTCGAAACGGCGCGAACCCATTATTTGAACCCCTCGCCGGCCACCACTTATTCGACGGGGACGGTATGGTTCACGCCGTCACGATCGACGGCGGCCGTGCCACTGCCAGTTATGCATGCCGGTTCACTGAAACGGAGAGACTTATTCAAGAACGCGCCATCGGCCGGCCTGTCTTCCCCAAGGCCATCGGCGAGCTCCACGGACATTCCGGCATTGCCCGTCTTCTTCTGTTCTTTACGAGGGGGCTATTCGGCCTTATAGACCACAGTCGCGGCACTGGAGTGGCGAACGCCGGCTTGGTTTACTTTAACGGCCGTATTCTGGCCATGTCGGAGGACGACCTTCCGTATCACGTTCGTATCACCCCCTCCGGCGACCTCGTAACCGCCGGGCGATATAACTTTGACGACCAGCTCAACTCTCCGATGATCGCTCACCCCAAAGTCGACCCCGATTCCGGAGAGCTCTTTACCCTCAGCTACAACTTCGTTCAGAAGCCATACCTGAAATACTTCTGGTTCTCACGGGATGGGAAAATGTTGCAGGAAGTCGAAATTCCGGTAGAAACTCCAACAATGATGCACGATTTCGCCATCACGGAGAATTTGGTAGTGATACCAGACCAGCAAGTCGTTTTCAATCTCCACAAAATGGTCAAAGGCGGCTCTCCGGTGATGTACGACGAGAAAAAGAAGTCCAGATTCGGGTTCCTACCGAAAAACGCTCGGGACTCCTCAAATATCATCTGGGTCGAATCGCCGGATACGTTCTGTTTCCACCTGTGGAATGCTTGGGAAGAAACAGAGTCGGAGGACGTCGTCGTGATCGGGTCATGCATGACGCCACCGGACTCCATTTTCAACGAACGCGACGAGGATTTGAAGGTCGTGCTTTCGGAGATCCGGTTCAATTTAAAGACCGGGGAATCAAGTCGGCGGCCGATAATTCCAGAATCGGAGCAAGTGAACCTGGAAGCCGGGATGGTGAACCGGAGGCGACTCGGGAGGAAAAGCCAGTACGCTTACCTGGCGATAACGGAACCATGGCCGAAAGTGTGTGGGTTCGCGAAGGTGGATGTTTCATCGGGAGAGATGAAGAAGTACATTTACGGCGAGCAGAGGTACGGCGGAGAGCCTTACTTCTTGGGAAGAGAGATGAGCTCCGAGAGAGAAGACGATGGTTATATACTGGTATTGGTGCACGACGAGAGGACGGGGAGATCAGAGCTTGAGATTGTGAACGCCATGAATATGGAGTTGGAAGCTTCTGTGAAGTTGCCGACGCGAGTACCGTACGGTTTCCATGGGACGTTTATCCATGCGAAGGACTTGGCAAATCAAGCTTAG

mRNA sequence

ATGCTCCTCCGTCTCTCCTCCTCCTCCTCCTCTGCCACCGTTGTTGCGGACTTGGGCCTGAACTTGGGCTTGGGCTTTCCCACAAGGCCCATTAACATTGGGAAGAAGCCCAATTGTTGCGCTCTAAACTCCCCGTCTGTGTTCCATTTCCCGAAACAGCCCAATAAACGACCACAAATTATGGAAGAGGACACCTCCAGGTGTCGTCAACCTCCGCACTGGAATTTTCTGCAACGAGGTGCGTCCTTCGCGCTCGACATGCTCGAACAAGCCTTGCTCTCCAATAACGACAACCGGAATCTTCCAAAAACCTTCGACCCACGTGTTCAAATCGCCGGGAACTACGCTCCGGTGCCGAACAACCATATCCAAGGCGTCTATCTTCGAAACGGCGCGAACCCATTATTTGAACCCCTCGCCGGCCACCACTTATTCGACGGGGACGGTATGGTTCACGCCGTCACGATCGACGGCGGCCGTGCCACTGCCAGTTATGCATGCCGGTTCACTGAAACGGAGAGACTTATTCAAGAACGCGCCATCGGCCGGCCTGTCTTCCCCAAGGCCATCGGCGAGCTCCACGGACATTCCGGCATTGCCCGTCTTCTTCTGTTCTTTACGAGGGGGCTATTCGGCCTTATAGACCACAGTCGCGGCACTGGAGTGGCGAACGCCGGCTTGGTTTACTTTAACGGCCGTATTCTGGCCATGTCGGAGGACGACCTTCCGTATCACGTTCGTATCACCCCCTCCGGCGACCTCGTAACCGCCGGGCGATATAACTTTGACGACCAGCTCAACTCTCCGATGATCGCTCACCCCAAAGTCGACCCCGATTCCGGAGAGCTCTTTACCCTCAGCTACAACTTCGTTCAGAAGCCATACCTGAAATACTTCTGGTTCTCACGGGATGGGAAAATGTTGCAGGAAGTCGAAATTCCGGTAGAAACTCCAACAATGATGCACGATTTCGCCATCACGGAGAATTTGGTAGTGATACCAGACCAGCAAGTCGTTTTCAATCTCCACAAAATGGTCAAAGGCGGCTCTCCGGTGATGTACGACGAGAAAAAGAAGTCCAGATTCGGGTTCCTACCGAAAAACGCTCGGGACTCCTCAAATATCATCTGGGTCGAATCGCCGGATACGTTCTGTTTCCACCTGTGGAATGCTTGGGAAGAAACAGAGTCGGAGGACGTCGTCGTGATCGGGTCATGCATGACGCCACCGGACTCCATTTTCAACGAACGCGACGAGGATTTGAAGGTCGTGCTTTCGGAGATCCGGTTCAATTTAAAGACCGGGGAATCAAGTCGGCGGCCGATAATTCCAGAATCGGAGCAAGTGAACCTGGAAGCCGGGATGGTGAACCGGAGGCGACTCGGGAGGAAAAGCCAGTACGCTTACCTGGCGATAACGGAACCATGGCCGAAAGTGTGTGGGTTCGCGAAGGTGGATGTTTCATCGGGAGAGATGAAGAAGTACATTTACGGCGAGCAGAGGTACGGCGGAGAGCCTTACTTCTTGGGAAGAGAGATGAGCTCCGAGAGAGAAGACGATGGTTATATACTGGTATTGGTGCACGACGAGAGGACGGGGAGATCAGAGCTTGAGATTGTGAACGCCATGAATATGGAGTTGGAAGCTTCTGTGAAGTTGCCGACGCGAGTACCGTACGGTTTCCATGGGACGTTTATCCATGCGAAGGACTTGGCAAATCAAGCTTAG

Coding sequence (CDS)

ATGCTCCTCCGTCTCTCCTCCTCCTCCTCCTCTGCCACCGTTGTTGCGGACTTGGGCCTGAACTTGGGCTTGGGCTTTCCCACAAGGCCCATTAACATTGGGAAGAAGCCCAATTGTTGCGCTCTAAACTCCCCGTCTGTGTTCCATTTCCCGAAACAGCCCAATAAACGACCACAAATTATGGAAGAGGACACCTCCAGGTGTCGTCAACCTCCGCACTGGAATTTTCTGCAACGAGGTGCGTCCTTCGCGCTCGACATGCTCGAACAAGCCTTGCTCTCCAATAACGACAACCGGAATCTTCCAAAAACCTTCGACCCACGTGTTCAAATCGCCGGGAACTACGCTCCGGTGCCGAACAACCATATCCAAGGCGTCTATCTTCGAAACGGCGCGAACCCATTATTTGAACCCCTCGCCGGCCACCACTTATTCGACGGGGACGGTATGGTTCACGCCGTCACGATCGACGGCGGCCGTGCCACTGCCAGTTATGCATGCCGGTTCACTGAAACGGAGAGACTTATTCAAGAACGCGCCATCGGCCGGCCTGTCTTCCCCAAGGCCATCGGCGAGCTCCACGGACATTCCGGCATTGCCCGTCTTCTTCTGTTCTTTACGAGGGGGCTATTCGGCCTTATAGACCACAGTCGCGGCACTGGAGTGGCGAACGCCGGCTTGGTTTACTTTAACGGCCGTATTCTGGCCATGTCGGAGGACGACCTTCCGTATCACGTTCGTATCACCCCCTCCGGCGACCTCGTAACCGCCGGGCGATATAACTTTGACGACCAGCTCAACTCTCCGATGATCGCTCACCCCAAAGTCGACCCCGATTCCGGAGAGCTCTTTACCCTCAGCTACAACTTCGTTCAGAAGCCATACCTGAAATACTTCTGGTTCTCACGGGATGGGAAAATGTTGCAGGAAGTCGAAATTCCGGTAGAAACTCCAACAATGATGCACGATTTCGCCATCACGGAGAATTTGGTAGTGATACCAGACCAGCAAGTCGTTTTCAATCTCCACAAAATGGTCAAAGGCGGCTCTCCGGTGATGTACGACGAGAAAAAGAAGTCCAGATTCGGGTTCCTACCGAAAAACGCTCGGGACTCCTCAAATATCATCTGGGTCGAATCGCCGGATACGTTCTGTTTCCACCTGTGGAATGCTTGGGAAGAAACAGAGTCGGAGGACGTCGTCGTGATCGGGTCATGCATGACGCCACCGGACTCCATTTTCAACGAACGCGACGAGGATTTGAAGGTCGTGCTTTCGGAGATCCGGTTCAATTTAAAGACCGGGGAATCAAGTCGGCGGCCGATAATTCCAGAATCGGAGCAAGTGAACCTGGAAGCCGGGATGGTGAACCGGAGGCGACTCGGGAGGAAAAGCCAGTACGCTTACCTGGCGATAACGGAACCATGGCCGAAAGTGTGTGGGTTCGCGAAGGTGGATGTTTCATCGGGAGAGATGAAGAAGTACATTTACGGCGAGCAGAGGTACGGCGGAGAGCCTTACTTCTTGGGAAGAGAGATGAGCTCCGAGAGAGAAGACGATGGTTATATACTGGTATTGGTGCACGACGAGAGGACGGGGAGATCAGAGCTTGAGATTGTGAACGCCATGAATATGGAGTTGGAAGCTTCTGTGAAGTTGCCGACGCGAGTACCGTACGGTTTCCATGGGACGTTTATCCATGCGAAGGACTTGGCAAATCAAGCTTAG

Protein sequence

MLLRLSSSSSSATVVADLGLNLGLGFPTRPINIGKKPNCCALNSPSVFHFPKQPNKRPQIMEEDTSRCRQPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAGNYAPVPNNHIQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGGRATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRGTGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPDSGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVVFNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESPDTFCFHLWNAWEETESEDVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNRRRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREMSSEREDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA

Homology

[Close]

BLAST of Sgr019437 vs. NCBI nr
Match: XP_038894014.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 925.2 bits (2390), Expect = 2.7e-265
Identity = 445/580 (76.72%), Postives = 497/580 (85.69%), Query Frame = 0


Query: 20  LNLGLGFPTRPINI-GKKPN------CCALNSPSVFHFPKQPNKRPQIMEEDTSRCRQPP 79
           + LGLG PTRPINI  KKPN         LNSPSVFHFPKQPNK+P+I E+ T +CR P 
Sbjct: 1   MGLGLGSPTRPINIVKKKPNQRNEVISSPLNSPSVFHFPKQPNKQPRITEDVTFKCRHPS 60

Query: 80  HWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAGNYAPVPNNH---------- 139
           HWN LQ+GAS A D+LEQA L NNDNRNLPKTFDP VQIAGNYAPVP +           
Sbjct: 61  HWNLLQKGASMAFDILEQAFL-NNDNRNLPKTFDPHVQIAGNYAPVPEHPVSHSLPVTGV 120

Query: 140 ----IQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGGRATASYACRFTETERLIQE 199
               I GVYLRNGANP FEPLAGHHLFDGDGM+HAVTI GGR  ASYACRFTETERL+QE
Sbjct: 121 IPDCINGVYLRNGANPFFEPLAGHHLFDGDGMIHAVTIGGGR--ASYACRFTETERLVQE 180

Query: 200 RAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRGTGVANAGLVYFNGRILAMS 259
           +AIGRPVFPKA+GELHGHSGIARLLLF+ RGLFGLIDH  GTGVANAGLVYFNGR+LAMS
Sbjct: 181 KAIGRPVFPKAVGELHGHSGIARLLLFYARGLFGLIDHHHGTGVANAGLVYFNGRLLAMS 240

Query: 260 EDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPDSGELFTLSYNFVQKPYLKY 319
           EDDLPYHVRITPSGDL+TAGRYNF+ QLNSPM+AHPK+DPD+GELF LSYNF  KPYLKY
Sbjct: 241 EDDLPYHVRITPSGDLITAGRYNFEGQLNSPMVAHPKIDPDTGELFALSYNFTHKPYLKY 300

Query: 320 FWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVVFNLHKMVKGGSPVMYDEKK 379
           F FS DGK+  E+EIPVE PTMMHDFAITEN +VIPDQQV+FNL KM+ GGSPVMYDEKK
Sbjct: 301 FQFSPDGKISPEIEIPVEIPTMMHDFAITENFIVIPDQQVIFNLKKMLTGGSPVMYDEKK 360

Query: 380 KSRFGFLPKNARDSSNIIWVESP-DTFCFHLWNAWEETESEDVVVIGSCMTPPDSIFNER 439
             RFGFL KNARDSSN+ WV+SP DTFCFHLWNAWEETES++++VIGSCMTPPDSIFNER
Sbjct: 361 NPRFGFLLKNARDSSNLTWVDSPADTFCFHLWNAWEETESDEIIVIGSCMTPPDSIFNER 420

Query: 440 DEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNRRRLGRKSQYAYLAITEPWP 499
           DE+ K VL+EIRFNL+TGES+RR II ESE VNLE G+VNR RLGRK++Y YLAITEPWP
Sbjct: 421 DEEFKAVLTEIRFNLRTGESARRAIISESEDVNLEVGVVNRNRLGRKTRYVYLAITEPWP 480

Query: 500 KVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREM--SSEREDDGYILVLVHDERTGRS 559
           K CGFAKVDVSSGE+KKYIYG++RYGGEP+FLGREM    EREDDGYI+VLVHDE+  RS
Sbjct: 481 KACGFAKVDVSSGEIKKYIYGDERYGGEPFFLGREMGCDCEREDDGYIVVLVHDEKKWRS 540

Query: 560 ELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
           EL+IVNA++++LEA V+LP+RVPYGFHGTF+HA DL +QA
Sbjct: 541 ELQIVNAIDLKLEACVELPSRVPYGFHGTFVHANDLTHQA 577

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BLAST of Sgr019437 vs. NCBI nr
Match: XP_022927145.1 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita moschata])

HSP 1 Score: 907.1 bits (2343), Expect = 7.5e-260
Identity = 446/599 (74.46%), Postives = 499/599 (83.31%), Query Frame = 0


Query: 1   MLLRLSSSSSSATVVADLGLNLGLGFPTRPINIGKKPN------CCALNSPSVFHFPKQP 60
           M + LSSSS+S      L L LGLGFPTRPI   KKPN       C LNSPSVFH+PKQP
Sbjct: 1   MAMALSSSSAS------LHLGLGLGFPTRPIR--KKPNLRNEVISCTLNSPSVFHYPKQP 60

Query: 61  NKRPQIMEEDTSRCR-QPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAG 120
           NKRP+I E+D S+CR  PP WN LQ+GAS A D+LEQALL N DN NLPK FDP VQIAG
Sbjct: 61  NKRPRITEDDASKCRHHPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNFDPHVQIAG 120

Query: 121 NYAPVPNNH--------------IQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGG 180
           NYAPVP                 I GVYLRNGANPLFEPL GHHLFDGDGM+HAVTI GG
Sbjct: 121 NYAPVPEQSVSHSLPVTGAIPDCINGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGG 180

Query: 181 RATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRG 240
               SYACRFTETERL+QER+IGRPVFPK IGELHGHSGIARLLLF+ RGLFGLIDH  G
Sbjct: 181 --CTSYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHG 240

Query: 241 TGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPD 300
           TGVANAGLVYFNG +LAMSE+DLPYHVRITPSGDLVTAGRYNF+ QL+SPM+AHPK+DPD
Sbjct: 241 TGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPD 300

Query: 301 SGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVV 360
           SGELF LSYNF  KPYL +F FS DGKM  ++EIPVE PTMMHDFAITEN VVIPDQQVV
Sbjct: 301 SGELFALSYNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVV 360

Query: 361 FNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESP-DTFCFHLWNAWEETESE 420
           FNL KM+ GGSPVMYD+KK  RFGFLPKNA DSSN+IWV+SP DTFCFHLWNAWEETES 
Sbjct: 361 FNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESN 420

Query: 421 DVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNR 480
           +VVVIGSCMTPPDSIFN +DEDLK +L+EIRFNL TG+S+RRPI+ + E+VNLE GMVN+
Sbjct: 421 EVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQ 480

Query: 481 RRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREMSS--E 540
             +GRK++YAYLAITEPWPK CGFAKVD+ SGE+KKYIYG+QRYGGEPYFLGRE+ S  E
Sbjct: 481 SLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCE 540

Query: 541 REDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
           REDDG+I+VLVHDE+  RSEL+IVNA++++LEASV+LP RVPYGFHGTF+HA  L +QA
Sbjct: 541 REDDGFIMVLVHDEKKWRSELQIVNAIDLKLEASVELPNRVPYGFHGTFVHASQLPHQA 588

[Close]

BLAST of Sgr019437 vs. NCBI nr
Match: XP_023001406.1 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita maxima])

HSP 1 Score: 906.7 bits (2342), Expect = 9.9e-260
Identity = 445/607 (73.31%), Postives = 504/607 (83.03%), Query Frame = 0


Query: 1   MLLRLSSSSSS--------ATVVADLGLNLGLGFPTRPINIGKKPN------CCALNSPS 60
           M + LSSSS+S           ++ + +  GLGF TRPI   KKPN       CALNSPS
Sbjct: 1   MAMALSSSSASPHFPFDRRLLEISTVAMGFGLGFLTRPIR--KKPNLRNEVIACALNSPS 60

Query: 61  VFHFPKQPNKRPQIMEEDTSRCR-QPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTF 120
           VFH+PKQPNKRP+I E+D S+CR +PP WN LQ+GAS A D+LEQALL N DN NLPK F
Sbjct: 61  VFHYPKQPNKRPRITEDDASKCRHRPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNF 120

Query: 121 DPRVQIAGNYAPVPNNH--------------IQGVYLRNGANPLFEPLAGHHLFDGDGMV 180
           DP VQIAGNYAPVP                 I GVYLRNGANPLF+PL GHHLFDGDGM+
Sbjct: 121 DPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCINGVYLRNGANPLFDPLGGHHLFDGDGMI 180

Query: 181 HAVTIDGGRATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLF 240
           HAVTI GG   ASYACRFTETERL+QER+IGRP+FPK IGELHGHSGIARLLLF+ RGLF
Sbjct: 181 HAVTIGGG--CASYACRFTETERLVQERSIGRPIFPKTIGELHGHSGIARLLLFYARGLF 240

Query: 241 GLIDHSRGTGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMI 300
           GLIDH  GTGVANAGLVYFNGR+LAMSE+DLPYHVRITPSGDLVTAGRYNF+  L+SPM+
Sbjct: 241 GLIDHRHGTGVANAGLVYFNGRLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGHLDSPMV 300

Query: 301 AHPKVDPDSGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLV 360
           AHPK+DPDSGELF LSYNF  KPYL +F FS DGKM  ++EIPVE PTMMHDFAITEN V
Sbjct: 301 AHPKIDPDSGELFALSYNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFV 360

Query: 361 VIPDQQVVFNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESP-DTFCFHLWN 420
           VIPDQQVVFNL KM+ GGSPVMYDEKK  RFGFLPKNA DSSN+IWV+SP DTFCFHLWN
Sbjct: 361 VIPDQQVVFNLRKMINGGSPVMYDEKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWN 420

Query: 421 AWEETESEDVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVN 480
           AWEETES +VVVIGSCMTPPDSIFN +DEDLK +L+EIRFNL TG+S+RRPI+ +SE+VN
Sbjct: 421 AWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKSEEVN 480

Query: 481 LEAGMVNRRRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLG 540
           LE GMVN+  +GRK++YAYLAITEPWPK CGFAKVD+ SGE+KKYIYG+QRYGGEPYFLG
Sbjct: 481 LEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLG 540

Query: 541 REMSS--EREDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHA 576
           RE+ S  EREDDG+I+VLVHDE+  RSEL+IVNA++++LEASV+LPTRVPYGFHGTF+HA
Sbjct: 541 REVYSDCEREDDGFIMVLVHDEKKWRSELQIVNAIDLKLEASVELPTRVPYGFHGTFVHA 600

[Close]

BLAST of Sgr019437 vs. NCBI nr
Match: XP_023520051.1 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 906.0 bits (2340), Expect = 1.7e-259
Identity = 445/599 (74.29%), Postives = 500/599 (83.47%), Query Frame = 0


Query: 1   MLLRLSSSSSSATVVADLGLNLGLGFPTRPINIGKKPN------CCALNSPSVFHFPKQP 60
           M + LSSSS+S        L  GLGFPTRPI   KKPN       CALNSPSVFH+PKQP
Sbjct: 1   MAMALSSSSASP------HLGFGLGFPTRPIR--KKPNLRNEVISCALNSPSVFHYPKQP 60

Query: 61  NKRPQIMEEDTSRCR-QPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAG 120
           +KRP+I E+D S+CR  PP WN LQ+GAS A D+LEQALL N DN NLPK FDP VQIAG
Sbjct: 61  SKRPRITEDDASKCRHHPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNFDPHVQIAG 120

Query: 121 NYAPVPNNH--------------IQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGG 180
           NYAPVP                 I GVYLRNGANPLFEPL GHHLFDGDGM+HAVTI GG
Sbjct: 121 NYAPVPEQSVSHSLPVTGAIPDCINGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGG 180

Query: 181 RATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRG 240
              ASYACRFTETERL+QER+IGRPVFPK IGELHGHSGIARLLLF+ RGLFGLIDH  G
Sbjct: 181 --CASYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHG 240

Query: 241 TGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPD 300
           TGVANAGLVYFNGR+LAMSE+DLPYHVRITPSGDLVTAGRYNF+ QL+SPM+AHPK+DPD
Sbjct: 241 TGVANAGLVYFNGRLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPD 300

Query: 301 SGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVV 360
           SGELF LSYNF  KPYL +F FS DGKM  ++EIPVE PTMMHDFAITEN VVIPDQQ+V
Sbjct: 301 SGELFALSYNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQIV 360

Query: 361 FNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESP-DTFCFHLWNAWEETESE 420
           FNL KM+ GGSPVMYDEKK  RFGFLPKNA DSSN+IWV+SP DTFCFHLWNAWEETES 
Sbjct: 361 FNLRKMINGGSPVMYDEKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESN 420

Query: 421 DVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNR 480
           +VVVIGSCMTPPDSIFN +DEDLK +L+EIRFNL TG+S+RRPI+ +SE+VNLE GMVN+
Sbjct: 421 EVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKSEEVNLEVGMVNQ 480

Query: 481 RRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREMSS--E 540
             +GRK++YAYLAITEPWPK CGFAKVD+ SGE+KKYIYG+Q YGGEPYFLGR++ S  E
Sbjct: 481 SLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQLYGGEPYFLGRDVYSDCE 540

Query: 541 REDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
           REDDG+I+V VHDE+  RSEL+IVNA++++LEASV+LPTRVPYGFHGTF+HA  L +QA
Sbjct: 541 REDDGFIMVFVHDEKKWRSELQIVNAIDLKLEASVELPTRVPYGFHGTFVHASHLPDQA 588

[Close]

BLAST of Sgr019437 vs. NCBI nr
Match: XP_008446856.1 (PREDICTED: probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucumis melo] >KAA0053987.1 putative 9-cis-epoxycarotenoid dioxygenase NCED5 [Cucumis melo var. makuwa])

HSP 1 Score: 892.1 bits (2304), Expect = 2.5e-255
Identity = 435/599 (72.62%), Postives = 492/599 (82.14%), Query Frame = 0


Query: 6   SSSSSSATVVAD-----LGLNLGLGFPTRPINI-GKKPN------CCALNSPSVFHFPKQ 65
           SSSS+S   V +     LGL LGLGFP  PINI  KKPN        ALNSP VFHFPKQ
Sbjct: 4   SSSSTSPHFVLEQKGLGLGLGLGLGFPKNPINIVKKKPNQRKEVISFALNSPFVFHFPKQ 63

Query: 66  PNKRPQIMEEDTSRCRQPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAG 125
            NK P+I E  TS+CR P HWN LQ+ AS A D+LEQ  L NNDN+NLPK FDP  QI G
Sbjct: 64  TNKPPRITEHHTSKCRHPSHWNLLQKVASTAFDILEQTFL-NNDNQNLPKNFDPHFQIVG 123

Query: 126 NYAPVPN--------------NHIQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGG 185
           NYAPVP               + I GVYLRNGANP F+PLAGHHLFDGDGM+HAVTI GG
Sbjct: 124 NYAPVPEQPVSHSLPVTGVIPDWINGVYLRNGANPYFQPLAGHHLFDGDGMIHAVTIGGG 183

Query: 186 RATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRG 245
           R  ASYACRFTETERL+QE++IGRPVFPKA+GELHGHSGIARLLLF+ RG FGLIDH RG
Sbjct: 184 R--ASYACRFTETERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGFFGLIDHRRG 243

Query: 246 TGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPD 305
           TGVANAGLVYFNGR+LAMSEDDLPY VRITPSGDL+T GRYNF+ QLNSPM+AHPK+DP+
Sbjct: 244 TGVANAGLVYFNGRLLAMSEDDLPYQVRITPSGDLITVGRYNFEGQLNSPMVAHPKIDPE 303

Query: 306 SGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVV 365
           SGELF LSYNF  KPYLK+F  + DGKM  +++IP++ PTM+HDFAITE  VVIPDQQVV
Sbjct: 304 SGELFALSYNFTHKPYLKHFRLTPDGKMSPKIDIPLDIPTMIHDFAITEKFVVIPDQQVV 363

Query: 366 FNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESP-DTFCFHLWNAWEETESE 425
           FNL KM+ GGSPVMYDEKK  RFGFLPKNA DSSN+ WV+SP DTFCFHLWNAWEETES+
Sbjct: 364 FNLQKMLTGGSPVMYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETESD 423

Query: 426 DVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNR 485
           DVVVI SCMTPPDSIFNERDE+ K VL+EIRFNL+TG+SS RPI+ ESE +NLE G+VNR
Sbjct: 424 DVVVIASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRPIVSESEHMNLEVGVVNR 483

Query: 486 RRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREM--SSE 545
           +RLGRK+QY YLAIT+PWPK CGFAKVDVSSGE+KKYIYG++RYGGEPYFLGREM    E
Sbjct: 484 KRLGRKTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGCE 543

Query: 546 REDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
            EDDGYI+V VHDE+  RSEL+IVNA++++LEA V+L +RVPYGFHGTF+HA DL +QA
Sbjct: 544 EEDDGYIMVFVHDEKEWRSELQIVNAIDLKLEACVELKSRVPYGFHGTFVHANDLRHQA 599

[Close]

BLAST of Sgr019437 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 1.0e-227
Identity = 387/588 (65.82%), Postives = 460/588 (78.23%), Query Frame = 0


Query: 4   RLSSSSSSATVVADLGLNLGLGFPTRPINIGKKPNCCALNSPSVFHFPKQ---PNKRPQI 63
           R+S S S +T       +L    P++ I++            ++   PKQ   P  +PQ 
Sbjct: 9   RVSHSFSPST-----SYSLDFTLPSKSISMKNHTTTKTKIHSALLTLPKQNNTPKNQPQF 68

Query: 64  MEEDTSRCRQPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAGNYAPVPN 123
                    Q  HWNF Q+ A+ ALD++E AL+S      LPKT DPRVQIAGN+APVP 
Sbjct: 69  ---------QTSHWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVPE 128

Query: 124 NH--------------IQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGGRATASYA 183
                           I GVY+RNGANPLFEP+AGHHLFDGDGMVHAVT++ G  + SY+
Sbjct: 129 QSVRHNLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTVENG--SVSYS 188

Query: 184 CRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRGTGVANAG 243
           CRFTETERL+QER +G PVFPKAIGELHGHSGIARLLLF+ RG+FGL+DHS GTGVANAG
Sbjct: 189 CRFTETERLVQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAG 248

Query: 244 LVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPDSGELFTL 303
           LV+FN R+LAMSEDD+PYHV++ PSGDL T GRYNFDDQL S MIAHPK+DP SGELF L
Sbjct: 249 LVFFNNRLLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFAL 308

Query: 304 SYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVVFNLHKMV 363
           SY+ VQKPYLK F FS DG+   +VEIP++ PTMMHDFAITEN VVIPDQQVVF L +M+
Sbjct: 309 SYDVVQKPYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMI 368

Query: 364 KGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESPDTFCFHLWNAWEETESEDVVVIGSC 423
           KGGSPV+YD+ KKSRFG LPKNA +S NIIWVES +TFCFHLWNAWEE E+++V+VIGSC
Sbjct: 369 KGGSPVIYDKNKKSRFGILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSC 428

Query: 424 MTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNRRRLGRKSQ 483
           MTPPDSIFNE +E+LK VLSEIR NLKTGES+RR ++  S+QVNLEAGMVNR +LGRK+Q
Sbjct: 429 MTPPDSIFNECNENLKSVLSEIRLNLKTGESTRRQLLSPSDQVNLEAGMVNRNKLGRKTQ 488

Query: 484 YAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREMSSEREDDGYILVL 543
           +AYLAI EPWPKV GFAKVD+S+GE+KK+IYG++RYGGEP FL R ++SE+EDDGYIL  
Sbjct: 489 FAYLAIAEPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAF 548

Query: 544 VHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQ 575
            HDE+T +SEL+IVNAM +ELEA+VKLP+RVPYGFHGTFI +KDL NQ
Sbjct: 549 CHDEKTWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQNQ 580

[Close]

BLAST of Sgr019437 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 769.2 bits (1985), Expect = 3.2e-221
Identity = 370/560 (66.07%), Postives = 445/560 (79.46%), Query Frame = 0


Query: 41  ALNSPSVFHFPKQPN----------KRPQIMEEDTSRCRQPPHWNFLQRGASFALDMLEQ 100
           +L +P + HFPKQ +            P+  E + S       WN +Q+ A+ ALD +E 
Sbjct: 49  SLQAPPILHFPKQSSNYQTPKNNTISHPK-QENNNSSSSSTSKWNLVQKAAAMALDAVES 108

Query: 101 ALLSNNDNRNLPKTFDPRVQIAGNYAPVPNNH--------------IQGVYLRNGANPLF 160
           AL  +     LPKT DPRVQI+GN+APVP N               +QGVY+RNGANPLF
Sbjct: 109 ALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLPVTGKIPKCVQGVYVRNGANPLF 168

Query: 161 EPLAGHHLFDGDGMVHAVTIDGGRATASYACRFTETERLIQERAIGRPVFPKAIGELHGH 220
           EP AGHH FDGDGMVHAV    G  +ASYACRFTETERL+QE+A+GRPVFPKAIGELHGH
Sbjct: 169 EPTAGHHFFDGDGMVHAVQFKNG--SASYACRFTETERLVQEKALGRPVFPKAIGELHGH 228

Query: 221 SGIARLLLFFTRGLFGLIDHSRGTGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVT 280
           SGIARL+LF+ RGLFGL+DHS+GTGVANAGLVYFN R+LAMSEDDLPYHV++TP+GDL T
Sbjct: 229 SGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKT 288

Query: 281 AGRYNFDDQLNSPMIAHPKVDPDSGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVE 340
            GR++FD QL S MIAHPK+DP SGELF LSY+ +QKPYLKYF FS++G+   +VEIPVE
Sbjct: 289 EGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVE 348

Query: 341 TPTMMHDFAITENLVVIPDQQVVFNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNII 400
            PTMMHDFAITEN VVIPDQQVVF + +M++GGSPV+YD+ K SRFG L K A+D S++ 
Sbjct: 349 DPTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSDLK 408

Query: 401 WVESPDTFCFHLWNAWEETESEDVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGE 460
           WVE PD FCFHLWNAWEE E++++VVIGSCMTPPDSIFNE DE LK VLSEIR NLKTG+
Sbjct: 409 WVEVPDCFCFHLWNAWEEAETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGK 468

Query: 461 SSRRPIIPE-SEQVNLEAGMVNRRRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKY 520
           S+R+ II    EQVNLEAGMVNR +LGRK++YAYLAI EPWPKV GFAKV++ +GE++K+
Sbjct: 469 STRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIAEPWPKVSGFAKVNLFTGEVEKF 528

Query: 521 IYGEQRYGGEPYFLGREMSSEREDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPT 576
           IYG+ +YGGEP FL R+ +S+ EDDGYIL  VHDE+  +SEL+IVNAM+++LEA+VKLP+
Sbjct: 529 IYGDNKYGGEPLFLPRDPNSKEEDDGYILAFVHDEKEWKSELQIVNAMSLKLEATVKLPS 588

[Close]

BLAST of Sgr019437 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 2.2e-214
Identity = 361/547 (66.00%), Postives = 432/547 (78.98%), Query Frame = 0


Query: 43  NSPSVFHFPKQPNKRPQIMEEDTSRCRQPPHWNFLQRGASFALDMLEQALLSNNDNRNLP 102
           ++P++ +FP  P+  P I E+DTSR      WN LQR AS ALD  E ALL    ++ LP
Sbjct: 51  DTPNLLNFPNYPSPNPIIPEKDTSR------WNPLQRAASAALDFAETALLRRERSKPLP 110

Query: 103 KTFDPRVQIAGNYAPVPNNH--------------IQGVYLRNGANPLFEPLAGHHLFDGD 162
           KT DPR QI+GNYAPVP                 I GVYLRNGANPLFEP++GHHLFDGD
Sbjct: 111 KTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGD 170

Query: 163 GMVHAVTIDGGRATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTR 222
           GMVHAV I  G   ASY+CRFTETERL+QE+ +G P+FPKAIGELHGHSGIARL+LF+ R
Sbjct: 171 GMVHAVKITNG--DASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYAR 230

Query: 223 GLFGLIDHSRGTGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNS 282
           GLFGL++H  GTGVANAGLVYF+ R+LAMSEDDLPY VR+T +GDL T GR++FD QL+S
Sbjct: 231 GLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSS 290

Query: 283 PMIAHPKVDPDSGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITE 342
            MIAHPK+DP + ELF LSY+ V+KPYLKYF FS +G+   +VEIP+ +PTMMHDFAITE
Sbjct: 291 AMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITE 350

Query: 343 NLVVIPDQQVVFNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESPDTFCFHL 402
           N VVIPDQQVVF L  M  G SPV YD +K SRFG LP+NA+D+S ++WVESP+TFCFHL
Sbjct: 351 NFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHL 410

Query: 403 WNAWEETESEDVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQ 462
           WNAWE  E+++VVVIGSCMTP DSIFNE DE L  VLSEIR NLKTG+S+RR IIP S Q
Sbjct: 411 WNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQ 470

Query: 463 VNLEAGMVNRRRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYF 522
           +NLEAGMVNR  LGRK++YAYLAI EPWPKV GFAKVD+S+GE+K + YG ++YGGEP+F
Sbjct: 471 MNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFF 530

Query: 523 LGREMSSEREDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHA 576
           L R + S+ EDDGYI+  VHDE +  SEL IVNA+ +ELEA+VKLP+RVPYGFHGTF+++
Sbjct: 531 LPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNS 589

[Close]

BLAST of Sgr019437 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.4e-211
Identity = 350/519 (67.44%), Postives = 420/519 (80.92%), Query Frame = 0


Query: 71  PPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAGNYAPVPNNH-------- 130
           P  WNFLQ+ A+  LDM+E AL+S+     LPKT DP+VQIAGN+APVP +         
Sbjct: 102 PQKWNFLQKAAATGLDMVETALVSHESKHPLPKTADPKVQIAGNFAPVPEHAADQALPVV 161

Query: 131 ------IQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGGRATASYACRFTETERLI 190
                 I GVY+RNGANPL+EP+AGHH FDGDGMVHAV    G   ASYACRFTET+RL 
Sbjct: 162 GKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFTNG--AASYACRFTETQRLA 221

Query: 191 QERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRGTGVANAGLVYFNGRILA 250
           QE+++GRPVFPKAIGELHGHSGIARLLLF+ R LF L+D S G GVANAGLVYFN  +LA
Sbjct: 222 QEKSLGRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNHLLA 281

Query: 251 MSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPDSGELFTLSYNFVQKPYL 310
           MSEDDLPYHVRIT +GDL T GRY+F+ QLNS MIAHPK+DP +G+L  LSY+ VQKPYL
Sbjct: 282 MSEDDLPYHVRITSNGDLTTVGRYDFNGQLNSTMIAHPKLDPVNGDLHALSYDVVQKPYL 341

Query: 311 KYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVVFNLHKMVKGGSPVMYDE 370
           KYF FS DG    +VEIP++ PTMMHDFAITEN VV+PDQQVVF L +M+ GGSPV+YD+
Sbjct: 342 KYFRFSADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPVVYDK 401

Query: 371 KKKSRFGFLPKNARDSSNIIWVESPDTFCFHLWNAWEETESEDVVVIGSCMTPPDSIFNE 430
            K SRFG L KNA+D++ + W+++P+ FCFHLWNAWEE E++++VVIGSCMTP DSIFNE
Sbjct: 402 NKTSRFGILDKNAKDANAMRWIDAPECFCFHLWNAWEEPETDEIVVIGSCMTPADSIFNE 461

Query: 431 RDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNRRRLGRKSQYAYLAITEPW 490
            DE LK VLSEIR NL+TG+S+RRPII ++EQVNLEAGMVNR +LGRK+Q+AYLA+ EPW
Sbjct: 462 CDESLKSVLSEIRLNLRTGKSTRRPIISDAEQVNLEAGMVNRNKLGRKTQFAYLALAEPW 521

Query: 491 PKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREMSSEREDDGYILVLVHDERTGRSE 550
           PKV GFAKVD+ SGE++KY+YGE+++GGEP FL    + E E DGYIL  VHDE+  +SE
Sbjct: 522 PKVSGFAKVDLFSGEVQKYMYGEEKFGGEPLFL---PNGEEEGDGYILAFVHDEKEWKSE 581

Query: 551 LEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
           L+IVNA N++LEAS+KLP+RVPYGFHGTFIH+KDL  QA
Sbjct: 582 LQIVNAQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQA 615

[Close]

BLAST of Sgr019437 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 8.8e-211
Identity = 359/562 (63.88%), Postives = 434/562 (77.22%), Query Frame = 0


Query: 28  TRPINIGKKPNCCALNSPSVFHFPKQPNKRPQIMEEDTSRCRQPPHWNFLQRGASFALDM 87
           TR +N+       AL++P   HFPKQ +  P I+ +  ++       N  QR A+ ALD 
Sbjct: 45  TRKLNVSS-----ALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDA 104

Query: 88  LEQALLSNNDNRNLPKTFDPRVQIAGNYAPVPN--------------NHIQGVYLRNGAN 147
            E  L+S+     LPKT DP VQIAGN+APV                + I+GVY+RNGAN
Sbjct: 105 AEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGAN 164

Query: 148 PLFEPLAGHHLFDGDGMVHAVTIDGGRATASYACRFTETERLIQERAIGRPVFPKAIGEL 207
           PL EP+ GHH FDGDGMVHAV  + G  +ASYACRFT+T R +QER +GRPVFPKAIGEL
Sbjct: 165 PLHEPVTGHHFFDGDGMVHAVKFEHG--SASYACRFTQTNRFVQERQLGRPVFPKAIGEL 224

Query: 208 HGHSGIARLLLFFTRGLFGLIDHSRGTGVANAGLVYFNGRILAMSEDDLPYHVRITPSGD 267
           HGH+GIARL+LF+ R   G++D + GTGVANAGLVYFNGR+LAMSEDDLPY V+ITP+GD
Sbjct: 225 HGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGD 284

Query: 268 LVTAGRYNFDDQLNSPMIAHPKVDPDSGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEI 327
           L T GR++FD QL S MIAHPKVDP+SGELF LSY+ V KPYLKYF FS DG    +VEI
Sbjct: 285 LKTVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI 344

Query: 328 PVETPTMMHDFAITENLVVIPDQQVVFNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSS 387
            ++ PTMMHDFAITEN VV+PDQQVVF L +M++GGSPV+YD+ K +RFG L K A DSS
Sbjct: 345 QLDQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSS 404

Query: 388 NIIWVESPDTFCFHLWNAWEETESEDVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLK 447
           NI W+++PD FCFHLWNAWEE E+++VVVIGSCMTPPDSIFNE DE+LK VLSEIR NLK
Sbjct: 405 NIKWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLK 464

Query: 448 TGESSRRPIIP-ESEQVNLEAGMVNRRRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEM 507
           TGES+RRPII  E +QVNLEAGMVNR  LGRK+++AYLA+ EPWPKV GFAKVD+++GE+
Sbjct: 465 TGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEV 524

Query: 508 KKYIYGEQRYGGEPYFLGREMSSEREDDGYILVLVHDERTGRSELEIVNAMNMELEASVK 567
           KK++YG+ RYGGEP FL  E     ED+GYIL  VHDE+T +SEL+IVNA+++E+EA+VK
Sbjct: 525 KKHLYGDNRYGGEPLFLPGE--GGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVK 584

Query: 568 LPTRVPYGFHGTFIHAKDLANQ 575
           LP+RVPYGFHGTFI A DLA Q
Sbjct: 585 LPSRVPYGFHGTFIGADDLAKQ 597

[Close]

BLAST of Sgr019437 vs. ExPASy TrEMBL
Match: A0A6J1EN25 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111434081 PE=3 SV=1)

HSP 1 Score: 907.1 bits (2343), Expect = 3.7e-260
Identity = 446/599 (74.46%), Postives = 499/599 (83.31%), Query Frame = 0


Query: 1   MLLRLSSSSSSATVVADLGLNLGLGFPTRPINIGKKPN------CCALNSPSVFHFPKQP 60
           M + LSSSS+S      L L LGLGFPTRPI   KKPN       C LNSPSVFH+PKQP
Sbjct: 1   MAMALSSSSAS------LHLGLGLGFPTRPIR--KKPNLRNEVISCTLNSPSVFHYPKQP 60

Query: 61  NKRPQIMEEDTSRCR-QPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAG 120
           NKRP+I E+D S+CR  PP WN LQ+GAS A D+LEQALL N DN NLPK FDP VQIAG
Sbjct: 61  NKRPRITEDDASKCRHHPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNFDPHVQIAG 120

Query: 121 NYAPVPNNH--------------IQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGG 180
           NYAPVP                 I GVYLRNGANPLFEPL GHHLFDGDGM+HAVTI GG
Sbjct: 121 NYAPVPEQSVSHSLPVTGAIPDCINGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGG 180

Query: 181 RATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRG 240
               SYACRFTETERL+QER+IGRPVFPK IGELHGHSGIARLLLF+ RGLFGLIDH  G
Sbjct: 181 --CTSYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHG 240

Query: 241 TGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPD 300
           TGVANAGLVYFNG +LAMSE+DLPYHVRITPSGDLVTAGRYNF+ QL+SPM+AHPK+DPD
Sbjct: 241 TGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPD 300

Query: 301 SGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVV 360
           SGELF LSYNF  KPYL +F FS DGKM  ++EIPVE PTMMHDFAITEN VVIPDQQVV
Sbjct: 301 SGELFALSYNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVV 360

Query: 361 FNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESP-DTFCFHLWNAWEETESE 420
           FNL KM+ GGSPVMYD+KK  RFGFLPKNA DSSN+IWV+SP DTFCFHLWNAWEETES 
Sbjct: 361 FNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESN 420

Query: 421 DVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNR 480
           +VVVIGSCMTPPDSIFN +DEDLK +L+EIRFNL TG+S+RRPI+ + E+VNLE GMVN+
Sbjct: 421 EVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQ 480

Query: 481 RRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREMSS--E 540
             +GRK++YAYLAITEPWPK CGFAKVD+ SGE+KKYIYG+QRYGGEPYFLGRE+ S  E
Sbjct: 481 SLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCE 540

Query: 541 REDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
           REDDG+I+VLVHDE+  RSEL+IVNA++++LEASV+LP RVPYGFHGTF+HA  L +QA
Sbjct: 541 REDDGFIMVLVHDEKKWRSELQIVNAIDLKLEASVELPNRVPYGFHGTFVHASQLPHQA 588

[Close]

BLAST of Sgr019437 vs. ExPASy TrEMBL
Match: A0A6J1KII7 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111495550 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 4.8e-260
Identity = 445/607 (73.31%), Postives = 504/607 (83.03%), Query Frame = 0


Query: 1   MLLRLSSSSSS--------ATVVADLGLNLGLGFPTRPINIGKKPN------CCALNSPS 60
           M + LSSSS+S           ++ + +  GLGF TRPI   KKPN       CALNSPS
Sbjct: 1   MAMALSSSSASPHFPFDRRLLEISTVAMGFGLGFLTRPIR--KKPNLRNEVIACALNSPS 60

Query: 61  VFHFPKQPNKRPQIMEEDTSRCR-QPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTF 120
           VFH+PKQPNKRP+I E+D S+CR +PP WN LQ+GAS A D+LEQALL N DN NLPK F
Sbjct: 61  VFHYPKQPNKRPRITEDDASKCRHRPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNF 120

Query: 121 DPRVQIAGNYAPVPNNH--------------IQGVYLRNGANPLFEPLAGHHLFDGDGMV 180
           DP VQIAGNYAPVP                 I GVYLRNGANPLF+PL GHHLFDGDGM+
Sbjct: 121 DPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCINGVYLRNGANPLFDPLGGHHLFDGDGMI 180

Query: 181 HAVTIDGGRATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLF 240
           HAVTI GG   ASYACRFTETERL+QER+IGRP+FPK IGELHGHSGIARLLLF+ RGLF
Sbjct: 181 HAVTIGGG--CASYACRFTETERLVQERSIGRPIFPKTIGELHGHSGIARLLLFYARGLF 240

Query: 241 GLIDHSRGTGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMI 300
           GLIDH  GTGVANAGLVYFNGR+LAMSE+DLPYHVRITPSGDLVTAGRYNF+  L+SPM+
Sbjct: 241 GLIDHRHGTGVANAGLVYFNGRLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGHLDSPMV 300

Query: 301 AHPKVDPDSGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLV 360
           AHPK+DPDSGELF LSYNF  KPYL +F FS DGKM  ++EIPVE PTMMHDFAITEN V
Sbjct: 301 AHPKIDPDSGELFALSYNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFV 360

Query: 361 VIPDQQVVFNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESP-DTFCFHLWN 420
           VIPDQQVVFNL KM+ GGSPVMYDEKK  RFGFLPKNA DSSN+IWV+SP DTFCFHLWN
Sbjct: 361 VIPDQQVVFNLRKMINGGSPVMYDEKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWN 420

Query: 421 AWEETESEDVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVN 480
           AWEETES +VVVIGSCMTPPDSIFN +DEDLK +L+EIRFNL TG+S+RRPI+ +SE+VN
Sbjct: 421 AWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKSEEVN 480

Query: 481 LEAGMVNRRRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLG 540
           LE GMVN+  +GRK++YAYLAITEPWPK CGFAKVD+ SGE+KKYIYG+QRYGGEPYFLG
Sbjct: 481 LEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLG 540

Query: 541 REMSS--EREDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHA 576
           RE+ S  EREDDG+I+VLVHDE+  RSEL+IVNA++++LEASV+LPTRVPYGFHGTF+HA
Sbjct: 541 REVYSDCEREDDGFIMVLVHDEKKWRSELQIVNAIDLKLEASVELPTRVPYGFHGTFVHA 600

[Close]

BLAST of Sgr019437 vs. ExPASy TrEMBL
Match: A0A5A7UHG6 (Putative 9-cis-epoxycarotenoid dioxygenase NCED5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G00330 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.2e-255
Identity = 435/599 (72.62%), Postives = 492/599 (82.14%), Query Frame = 0


Query: 6   SSSSSSATVVAD-----LGLNLGLGFPTRPINI-GKKPN------CCALNSPSVFHFPKQ 65
           SSSS+S   V +     LGL LGLGFP  PINI  KKPN        ALNSP VFHFPKQ
Sbjct: 4   SSSSTSPHFVLEQKGLGLGLGLGLGFPKNPINIVKKKPNQRKEVISFALNSPFVFHFPKQ 63

Query: 66  PNKRPQIMEEDTSRCRQPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAG 125
            NK P+I E  TS+CR P HWN LQ+ AS A D+LEQ  L NNDN+NLPK FDP  QI G
Sbjct: 64  TNKPPRITEHHTSKCRHPSHWNLLQKVASTAFDILEQTFL-NNDNQNLPKNFDPHFQIVG 123

Query: 126 NYAPVPN--------------NHIQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGG 185
           NYAPVP               + I GVYLRNGANP F+PLAGHHLFDGDGM+HAVTI GG
Sbjct: 124 NYAPVPEQPVSHSLPVTGVIPDWINGVYLRNGANPYFQPLAGHHLFDGDGMIHAVTIGGG 183

Query: 186 RATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRG 245
           R  ASYACRFTETERL+QE++IGRPVFPKA+GELHGHSGIARLLLF+ RG FGLIDH RG
Sbjct: 184 R--ASYACRFTETERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGFFGLIDHRRG 243

Query: 246 TGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPD 305
           TGVANAGLVYFNGR+LAMSEDDLPY VRITPSGDL+T GRYNF+ QLNSPM+AHPK+DP+
Sbjct: 244 TGVANAGLVYFNGRLLAMSEDDLPYQVRITPSGDLITVGRYNFEGQLNSPMVAHPKIDPE 303

Query: 306 SGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVV 365
           SGELF LSYNF  KPYLK+F  + DGKM  +++IP++ PTM+HDFAITE  VVIPDQQVV
Sbjct: 304 SGELFALSYNFTHKPYLKHFRLTPDGKMSPKIDIPLDIPTMIHDFAITEKFVVIPDQQVV 363

Query: 366 FNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESP-DTFCFHLWNAWEETESE 425
           FNL KM+ GGSPVMYDEKK  RFGFLPKNA DSSN+ WV+SP DTFCFHLWNAWEETES+
Sbjct: 364 FNLQKMLTGGSPVMYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETESD 423

Query: 426 DVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNR 485
           DVVVI SCMTPPDSIFNERDE+ K VL+EIRFNL+TG+SS RPI+ ESE +NLE G+VNR
Sbjct: 424 DVVVIASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRPIVSESEHMNLEVGVVNR 483

Query: 486 RRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREM--SSE 545
           +RLGRK+QY YLAIT+PWPK CGFAKVDVSSGE+KKYIYG++RYGGEPYFLGREM    E
Sbjct: 484 KRLGRKTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGCE 543

Query: 546 REDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
            EDDGYI+V VHDE+  RSEL+IVNA++++LEA V+L +RVPYGFHGTF+HA DL +QA
Sbjct: 544 EEDDGYIMVFVHDEKEWRSELQIVNAIDLKLEACVELKSRVPYGFHGTFVHANDLRHQA 599

[Close]

BLAST of Sgr019437 vs. ExPASy TrEMBL
Match: A0A1S3BG38 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489449 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.2e-255
Identity = 435/599 (72.62%), Postives = 492/599 (82.14%), Query Frame = 0


Query: 6   SSSSSSATVVAD-----LGLNLGLGFPTRPINI-GKKPN------CCALNSPSVFHFPKQ 65
           SSSS+S   V +     LGL LGLGFP  PINI  KKPN        ALNSP VFHFPKQ
Sbjct: 4   SSSSTSPHFVLEQKGLGLGLGLGLGFPKNPINIVKKKPNQRKEVISFALNSPFVFHFPKQ 63

Query: 66  PNKRPQIMEEDTSRCRQPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAG 125
            NK P+I E  TS+CR P HWN LQ+ AS A D+LEQ  L NNDN+NLPK FDP  QI G
Sbjct: 64  TNKPPRITEHHTSKCRHPSHWNLLQKVASTAFDILEQTFL-NNDNQNLPKNFDPHFQIVG 123

Query: 126 NYAPVPN--------------NHIQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGG 185
           NYAPVP               + I GVYLRNGANP F+PLAGHHLFDGDGM+HAVTI GG
Sbjct: 124 NYAPVPEQPVSHSLPVTGVIPDWINGVYLRNGANPYFQPLAGHHLFDGDGMIHAVTIGGG 183

Query: 186 RATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRG 245
           R  ASYACRFTETERL+QE++IGRPVFPKA+GELHGHSGIARLLLF+ RG FGLIDH RG
Sbjct: 184 R--ASYACRFTETERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGFFGLIDHRRG 243

Query: 246 TGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPD 305
           TGVANAGLVYFNGR+LAMSEDDLPY VRITPSGDL+T GRYNF+ QLNSPM+AHPK+DP+
Sbjct: 244 TGVANAGLVYFNGRLLAMSEDDLPYQVRITPSGDLITVGRYNFEGQLNSPMVAHPKIDPE 303

Query: 306 SGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVV 365
           SGELF LSYNF  KPYLK+F  + DGKM  +++IP++ PTM+HDFAITE  VVIPDQQVV
Sbjct: 304 SGELFALSYNFTHKPYLKHFRLTPDGKMSPKIDIPLDIPTMIHDFAITEKFVVIPDQQVV 363

Query: 366 FNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESP-DTFCFHLWNAWEETESE 425
           FNL KM+ GGSPVMYDEKK  RFGFLPKNA DSSN+ WV+SP DTFCFHLWNAWEETES+
Sbjct: 364 FNLQKMLTGGSPVMYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETESD 423

Query: 426 DVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNR 485
           DVVVI SCMTPPDSIFNERDE+ K VL+EIRFNL+TG+SS RPI+ ESE +NLE G+VNR
Sbjct: 424 DVVVIASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRPIVSESEHMNLEVGVVNR 483

Query: 486 RRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREM--SSE 545
           +RLGRK+QY YLAIT+PWPK CGFAKVDVSSGE+KKYIYG++RYGGEPYFLGREM    E
Sbjct: 484 KRLGRKTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGCE 543

Query: 546 REDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
            EDDGYI+V VHDE+  RSEL+IVNA++++LEA V+L +RVPYGFHGTF+HA DL +QA
Sbjct: 544 EEDDGYIMVFVHDEKEWRSELQIVNAIDLKLEACVELKSRVPYGFHGTFVHANDLRHQA 599

[Close]

BLAST of Sgr019437 vs. ExPASy TrEMBL
Match: A0A0A0M030 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G435760 PE=3 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 7.6e-250
Identity = 426/596 (71.48%), Postives = 485/596 (81.38%), Query Frame = 0


Query: 6   SSSSSSATVVADLGLNLGLGFPTRPINI-GKKPN--------CCALNSPSVFHFPKQPNK 65
           SS+S+S   V D     GLGFP +PINI  KKPN           +NSP VFHFPKQPNK
Sbjct: 4   SSTSTSPHFVLD---QKGLGFPIKPINIVKKKPNQRKEVISSALKVNSPFVFHFPKQPNK 63

Query: 66  RPQIMEEDTSRCRQPPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAGNYA 125
            P+I E  T + R P HWN LQ+ AS A D+LEQA L NN NRNLPK FDP  QI GNYA
Sbjct: 64  GPRITEHYTFKSRHPSHWNLLQKVASTAFDILEQAFL-NNHNRNLPKNFDPHFQIVGNYA 123

Query: 126 PVPN--------------NHIQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGGRAT 185
           PVP               + I GVYLRNGANP F+PL+GHHLFDGDGM+HAVTI  GR  
Sbjct: 124 PVPEQPVSHSLPVTGVIPHWINGVYLRNGANPFFQPLSGHHLFDGDGMIHAVTIGEGR-- 183

Query: 186 ASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRGTGV 245
           ASYACRFT+TERL+QE++IGRPVFPKA+GELHGHSGIARLLLF+ RGLFGLIDH RGTGV
Sbjct: 184 ASYACRFTQTERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGLFGLIDHRRGTGV 243

Query: 246 ANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPDSGE 305
           ANAGLVYFNGR+LAMSEDDLPY VRITPSGDL+T GRYNF+ QLNSPM+AHPK+DPDSGE
Sbjct: 244 ANAGLVYFNGRLLAMSEDDLPYQVRITPSGDLITIGRYNFEGQLNSPMVAHPKIDPDSGE 303

Query: 306 LFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVVFNL 365
           LF LSYNF  KPYLK+F  + DGKM  E++IP+E PTM+HDFAITE  VVIPDQQVVFNL
Sbjct: 304 LFALSYNFTHKPYLKHFRLTPDGKMSPEIDIPLEIPTMIHDFAITEKFVVIPDQQVVFNL 363

Query: 366 HKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESP-DTFCFHLWNAWEETESEDVV 425
            KM+ GGSPV+YDEKK  RFGFLPKNA DSSN+ WV+SP DTFCFHLWNAWEETES+D+V
Sbjct: 364 QKMLSGGSPVVYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETESDDIV 423

Query: 426 VIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNRRRL 485
           VI SCMTPPDSIFNERDE+ K VL+EIRFNL+TG+SS R II ESE VNLE G+VNR+R+
Sbjct: 424 VIASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRRIISESEHVNLEVGVVNRKRV 483

Query: 486 GRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREM--SSERED 545
           GRK+QY YLAIT+PWPK CGFAKVDVSSGE+KKYIYG++RYGGEPYFLGREM    E ED
Sbjct: 484 GRKTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGCEEED 543

Query: 546 DGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
           DG+I+V VHDE+  RSEL++VNA +++LEA V+L +RVPYGFHGTF+HA DL +QA
Sbjct: 544 DGHIMVFVHDEKKWRSELQVVNATDLKLEACVELSSRVPYGFHGTFVHANDLIHQA 593

[Close]

BLAST of Sgr019437 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 746.5 bits (1926), Expect = 1.6e-215
Identity = 361/547 (66.00%), Postives = 432/547 (78.98%), Query Frame = 0


Query: 43  NSPSVFHFPKQPNKRPQIMEEDTSRCRQPPHWNFLQRGASFALDMLEQALLSNNDNRNLP 102
           ++P++ +FP  P+  P I E+DTSR      WN LQR AS ALD  E ALL    ++ LP
Sbjct: 51  DTPNLLNFPNYPSPNPIIPEKDTSR------WNPLQRAASAALDFAETALLRRERSKPLP 110

Query: 103 KTFDPRVQIAGNYAPVPNNH--------------IQGVYLRNGANPLFEPLAGHHLFDGD 162
           KT DPR QI+GNYAPVP                 I GVYLRNGANPLFEP++GHHLFDGD
Sbjct: 111 KTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGD 170

Query: 163 GMVHAVTIDGGRATASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTR 222
           GMVHAV I  G   ASY+CRFTETERL+QE+ +G P+FPKAIGELHGHSGIARL+LF+ R
Sbjct: 171 GMVHAVKITNG--DASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYAR 230

Query: 223 GLFGLIDHSRGTGVANAGLVYFNGRILAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNS 282
           GLFGL++H  GTGVANAGLVYF+ R+LAMSEDDLPY VR+T +GDL T GR++FD QL+S
Sbjct: 231 GLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSS 290

Query: 283 PMIAHPKVDPDSGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITE 342
            MIAHPK+DP + ELF LSY+ V+KPYLKYF FS +G+   +VEIP+ +PTMMHDFAITE
Sbjct: 291 AMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITE 350

Query: 343 NLVVIPDQQVVFNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSSNIIWVESPDTFCFHL 402
           N VVIPDQQVVF L  M  G SPV YD +K SRFG LP+NA+D+S ++WVESP+TFCFHL
Sbjct: 351 NFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHL 410

Query: 403 WNAWEETESEDVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQ 462
           WNAWE  E+++VVVIGSCMTP DSIFNE DE L  VLSEIR NLKTG+S+RR IIP S Q
Sbjct: 411 WNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQ 470

Query: 463 VNLEAGMVNRRRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYF 522
           +NLEAGMVNR  LGRK++YAYLAI EPWPKV GFAKVD+S+GE+K + YG ++YGGEP+F
Sbjct: 471 MNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFF 530

Query: 523 LGREMSSEREDDGYILVLVHDERTGRSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHA 576
           L R + S+ EDDGYI+  VHDE +  SEL IVNA+ +ELEA+VKLP+RVPYGFHGTF+++
Sbjct: 531 LPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNS 589

[Close]

BLAST of Sgr019437 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 734.6 bits (1895), Expect = 6.2e-212
Identity = 359/562 (63.88%), Postives = 434/562 (77.22%), Query Frame = 0


Query: 28  TRPINIGKKPNCCALNSPSVFHFPKQPNKRPQIMEEDTSRCRQPPHWNFLQRGASFALDM 87
           TR +N+       AL++P   HFPKQ +  P I+ +  ++       N  QR A+ ALD 
Sbjct: 45  TRKLNVSS-----ALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDA 104

Query: 88  LEQALLSNNDNRNLPKTFDPRVQIAGNYAPVPN--------------NHIQGVYLRNGAN 147
            E  L+S+     LPKT DP VQIAGN+APV                + I+GVY+RNGAN
Sbjct: 105 AEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGAN 164

Query: 148 PLFEPLAGHHLFDGDGMVHAVTIDGGRATASYACRFTETERLIQERAIGRPVFPKAIGEL 207
           PL EP+ GHH FDGDGMVHAV  + G  +ASYACRFT+T R +QER +GRPVFPKAIGEL
Sbjct: 165 PLHEPVTGHHFFDGDGMVHAVKFEHG--SASYACRFTQTNRFVQERQLGRPVFPKAIGEL 224

Query: 208 HGHSGIARLLLFFTRGLFGLIDHSRGTGVANAGLVYFNGRILAMSEDDLPYHVRITPSGD 267
           HGH+GIARL+LF+ R   G++D + GTGVANAGLVYFNGR+LAMSEDDLPY V+ITP+GD
Sbjct: 225 HGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGD 284

Query: 268 LVTAGRYNFDDQLNSPMIAHPKVDPDSGELFTLSYNFVQKPYLKYFWFSRDGKMLQEVEI 327
           L T GR++FD QL S MIAHPKVDP+SGELF LSY+ V KPYLKYF FS DG    +VEI
Sbjct: 285 LKTVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI 344

Query: 328 PVETPTMMHDFAITENLVVIPDQQVVFNLHKMVKGGSPVMYDEKKKSRFGFLPKNARDSS 387
            ++ PTMMHDFAITEN VV+PDQQVVF L +M++GGSPV+YD+ K +RFG L K A DSS
Sbjct: 345 QLDQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSS 404

Query: 388 NIIWVESPDTFCFHLWNAWEETESEDVVVIGSCMTPPDSIFNERDEDLKVVLSEIRFNLK 447
           NI W+++PD FCFHLWNAWEE E+++VVVIGSCMTPPDSIFNE DE+LK VLSEIR NLK
Sbjct: 405 NIKWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLK 464

Query: 448 TGESSRRPIIP-ESEQVNLEAGMVNRRRLGRKSQYAYLAITEPWPKVCGFAKVDVSSGEM 507
           TGES+RRPII  E +QVNLEAGMVNR  LGRK+++AYLA+ EPWPKV GFAKVD+++GE+
Sbjct: 465 TGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEV 524

Query: 508 KKYIYGEQRYGGEPYFLGREMSSEREDDGYILVLVHDERTGRSELEIVNAMNMELEASVK 567
           KK++YG+ RYGGEP FL  E     ED+GYIL  VHDE+T +SEL+IVNA+++E+EA+VK
Sbjct: 525 KKHLYGDNRYGGEPLFLPGE--GGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVK 584

Query: 568 LPTRVPYGFHGTFIHAKDLANQ 575
           LP+RVPYGFHGTFI A DLA Q
Sbjct: 585 LPSRVPYGFHGTFIGADDLAKQ 597

[Close]

BLAST of Sgr019437 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 722.2 bits (1863), Expect = 3.2e-208
Identity = 351/521 (67.37%), Postives = 419/521 (80.42%), Query Frame = 0


Query: 70  QPPHWNFLQRGASFALDMLEQALLSNNDNRN-LPKTFDPRVQIAGNYAPVPNNH------ 129
           Q P  N +QR A+  LD +E A++S+   R+  PKT DP VQIAGN+ PVP         
Sbjct: 141 QNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNLP 200

Query: 130 --------IQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGGRATASYACRFTETER 189
                   IQGVY+RNGANPL +P++GHHLFDGDGMVHAV  D G  + SYACRFTET R
Sbjct: 201 VTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRFDNG--SVSYACRFTETNR 260

Query: 190 LIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRGTGVANAGLVYFNGRI 249
           L+QER  GRPVFPKAIGELHGH GIA+L+LF TRGLFGL+D + G GVANAGLVYFNG +
Sbjct: 261 LVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHL 320

Query: 250 LAMSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPDSGELFTLSYNFVQKP 309
           LAMSEDDLPYHV++T +GDL T+GRY+FD QL S MIAHPK+DP++ ELF LSY+ V KP
Sbjct: 321 LAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALSYDVVSKP 380

Query: 310 YLKYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVVFNLHKMVKGGSPVMY 369
           YLKYF F+ DG+   +VEIP++ PTM+HDFAITEN VVIPDQQVVF L +M++GGSPV+Y
Sbjct: 381 YLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVY 440

Query: 370 DEKKKSRFGFLPKNARDSSNIIWVESPDTFCFHLWNAWEETESEDVVVIGSCMTPPDSIF 429
           DEKKKSRFG L KNA+D+S+I W+E PD FCFHLWN+WEE E+++VVVIGSCMTPPDSIF
Sbjct: 441 DEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCMTPPDSIF 500

Query: 430 NERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNRRRLGRKSQYAYLAITE 489
           NE DE L+ VLSEIR NLKTGES+RRP+I  SEQVNLEAGMVNR  LGRK++YAYLA+TE
Sbjct: 501 NEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNRNLLGRKTRYAYLALTE 560

Query: 490 PWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREMSSEREDDGYILVLVHDERTGR 549
           PWPKV GFAKVD+S+GE++KYIYGE +YGGEP FL        ED GYI+V VHDE   +
Sbjct: 561 PWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFL--PSGDGEEDGGYIMVFVHDEEKVK 620

Query: 550 SELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQA 576
           SEL+++NA+NM+LEA+V LP+RVPYGFHGTFI  +DL+ QA
Sbjct: 621 SELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQA 655

[Close]

BLAST of Sgr019437 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 697.2 bits (1798), Expect = 1.1e-200
Identity = 335/520 (64.42%), Postives = 411/520 (79.04%), Query Frame = 0


Query: 71  PPHWNFLQRGASFALDMLEQALLSNNDNRNLPKTFDPRVQIAGNYAPVPNNH-------- 130
           P   N  Q+ A+ A+D  E+AL+S+  +  LPKT DPRVQIAGNY+PVP +         
Sbjct: 66  PLRLNIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVE 125

Query: 131 ------IQGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGGRATASYACRFTETERLI 190
                 I GVY+RNGANP+FEP AGHHLFDGDGMVHAV I  G  +ASYACRFT+TERL+
Sbjct: 126 GTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNG--SASYACRFTKTERLV 185

Query: 191 QERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRGTGVANAGLVYFNGRILA 250
           QE+ +GRPVFPKAIGELHGHSGIARL+LF+ RGL GLI++  G GVANAGLVYFN R+LA
Sbjct: 186 QEKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLA 245

Query: 251 MSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPDSGELFTLSYNFVQKPYL 310
           MSEDDLPY ++IT +GDL T GRY+FD QL S MIAHPK+DP + EL  LSY+ V+KPYL
Sbjct: 246 MSEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYL 305

Query: 311 KYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVVFNLHKMVKGGSPVMYDE 370
           KYF FS DG    E+EIP+ETPTM+HDFAITEN VVIPDQQVVF L +M+ G SPV++D 
Sbjct: 306 KYFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDG 365

Query: 371 KKKSRFGFLPKNARDSSNIIWVESPDTFCFHLWNAWEETESEDVVVIGSCMTPPDSIFNE 430
           +K SR G +PK+A ++S IIWV SP+TFCFHLWNAWE  E+E++VVIGSCM+P DSIFNE
Sbjct: 366 EKVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNE 425

Query: 431 RDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNRRRLGRKSQYAYLAITEPW 490
           RDE L+ VLSEIR NL+T +++RR ++  +E VNLE GMVNR RLGRK+++A+LAI  PW
Sbjct: 426 RDESLRSVLSEIRINLRTRKTTRRSLL-VNEDVNLEIGMVNRNRLGRKTRFAFLAIAYPW 485

Query: 491 PKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREMSS--EREDDGYILVLVHDERTGR 550
           PKV GFAKVD+ +GEMKKYIYG ++YGGEP+FL     +  E EDDGYI   VHDE T  
Sbjct: 486 PKVSGFAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKT 545

Query: 551 SELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQ 575
           SEL+I+NA+N++LEA++KLP+RVPYGFHGTF+ + +L +Q
Sbjct: 546 SELQIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

[Close]

BLAST of Sgr019437 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 580.5 bits (1495), Expect = 1.5e-165
Identity = 288/521 (55.28%), Postives = 371/521 (71.21%), Query Frame = 0


Query: 72  PHWNFLQRGASFALDMLEQAL-LSNNDNRNLPKTFDPRVQIAGNYAPVPNNHIQ------ 131
           P+ N LQ+ A+  LD +E ++ +    NR LPK  DP VQ++GN+APV    +Q      
Sbjct: 61  PNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVV 120

Query: 132 --------GVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIDGGRATASYACRFTETERLI 191
                   GVY+RNGANP+F PLAGHHLFDGDGM+HAV+I G     SY+CR+T+T RL+
Sbjct: 121 GQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSI-GFDNQVSYSCRYTKTNRLV 180

Query: 192 QERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHSRGTGVANAGLVYFNGRILA 251
           QE A+GR VFPK IGELHGHSG+ARL LF  R   GL+D +RG GVANAG+V+FNGR+LA
Sbjct: 181 QETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGRLLA 240

Query: 252 MSEDDLPYHVRITPSGDLVTAGRYNFDDQLNSPMIAHPKVDPDSGELFTLSYNFVQKPYL 311
           MSEDDLPY V+I   GDL T GR+ FDDQ++S +IAHPKVD  +G+L TLSYN ++KP+L
Sbjct: 241 MSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKKPHL 300

Query: 312 KYFWFSRDGKMLQEVEIPVETPTMMHDFAITENLVVIPDQQVVFNLHKMVKGGSPVMYDE 371
           +Y  F+  GK  ++VEI +  PTM+HDFAITEN VVIPDQQ+VF L +M++GGSPV+Y +
Sbjct: 301 RYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYVK 360

Query: 372 KKKSRFGFLPKNARDSSNIIWVESPDTFCFHLWNAWEE-TESED--VVVIGSCMTPPDSI 431
           +K +RFG L K     S+I WV+ PD FCFHLWNAWEE TE  D  +VVIGSCM+PPD+I
Sbjct: 361 EKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTI 420

Query: 432 FNERDEDLKVVLSEIRFNLKTGESSRRPIIPESEQVNLEAGMVNRRRLGRKSQYAYLAIT 491
           F+E  E  +V LSEIR N++T ES+R+ I+     VNLEAG +NR  +GRKSQ+ Y+AI 
Sbjct: 421 FSESGEPTRVELSEIRLNMRTKESNRKVIV---TGVNLEAGHINRSYVGRKSQFVYIAIA 480

Query: 492 EPWPKVCGFAKVDVSSGEMKKYIYGEQRYGGEPYFLGREMSSEREDDGYILVLVHDERTG 551
           +PWPK  G AKVD+ +G + ++ YG  R+GGEP F+        ED GY++  V DE   
Sbjct: 481 DPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE--GEGEEDKGYVMGFVRDEEKD 540

Query: 552 RSELEIVNAMNMELEASVKLPTRVPYGFHGTFIHAKDLANQ 575
            SE  +V+A +M+  A+V+LP RVPYGFHGTF+    L  Q
Sbjct: 541 ESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

The following BLAST results are available for this feature:

BLAST of Sgr019437 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038894014.1	2.7e-265	76.72	9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida]	[more]
XP_022927145.1	7.5e-260	74.46	probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita mosch...	[more]
XP_023001406.1	9.9e-260	73.31	probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita maxim...	[more]
XP_023520051.1	1.7e-259	74.29	probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita pepo ...	[more]
XP_008446856.1	2.5e-255	72.62	PREDICTED: probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucu...	[more]

BLAST of Sgr019437 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
K4CJJ1	1.0e-227	65.82	9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O...	[more]
O24023	3.2e-221	66.07	9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O...	[more]
Q9C6Z1	2.2e-214	66.00	Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis t...	[more]
Q9M6E8	1.4e-211	67.44	9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=...	[more]
Q9LRR7	8.8e-211	63.88	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O...	[more]

BLAST of Sgr019437 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EN25	3.7e-260	74.46	probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita mos...	[more]
A0A6J1KII7	4.8e-260	73.31	probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita max...	[more]
A0A5A7UHG6	1.2e-255	72.62	Putative 9-cis-epoxycarotenoid dioxygenase NCED5 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BG38	1.2e-255	72.62	probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucumis melo ...	[more]
A0A0A0M030	7.6e-250	71.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G435760 PE=3 SV=1	[more]

BLAST of Sgr019437 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30100.1	1.6e-215	66.00	nine-cis-epoxycarotenoid dioxygenase 5	[more]
AT3G14440.1	6.2e-212	63.88	nine-cis-epoxycarotenoid dioxygenase 3	[more]
AT1G78390.1	3.2e-208	67.37	nine-cis-epoxycarotenoid dioxygenase 9	[more]
AT4G18350.1	1.1e-200	64.42	nine-cis-epoxycarotenoid dioxygenase 2	[more]
AT3G24220.1	1.5e-165	55.28	nine-cis-epoxycarotenoid dioxygenase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004294	Carotenoid oxygenase	PFAM	PF03055	RPE65	coord: 122..566 e-value: 4.1E-106 score: 355.9
IPR004294	Carotenoid oxygenase	PANTHER	PTHR10543	BETA-CAROTENE DIOXYGENASE	coord: 38..575
None	No IPR available	PANTHER	PTHR10543:SF123	9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATED	coord: 38..575

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr019437.1	Sgr019437.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016121	carotene catabolic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0010436	carotenoid dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen