Homology
BLAST of Sgr019312 vs. NCBI nr
Match:
XP_022156755.1 (asparagine--tRNA ligase, cytoplasmic 2 [Momordica charantia])
HSP 1 Score: 1068.9 bits (2763), Expect = 1.6e-308
Identity = 544/620 (87.74%), Postives = 578/620 (93.23%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKE---P 60
MAAQQPA FTP FSKYSKRVLLK++LES+DGGLGFV QK+V+GGWVKSSKEVV++ P
Sbjct: 1 MAAQQPAAFTPAAFSKYSKRVLLKSVLESADGGLGFVDQKLVVGGWVKSSKEVVRDTARP 60
Query: 61 PPPAPSGERPAAEAPKAKDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPL 120
PP + + P+AKDVSCIEIIQSRIP VRSLL+ILYGN++ GEKLETPVHKPPL
Sbjct: 61 PPDSGAAVEALPPPPRAKDVSCIEIIQSRIPIVRSLLRILYGNSFHAGEKLETPVHKPPL 120
Query: 121 PSIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVE 180
PSIAFL VNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEG+LKR SV GKHVV+L+V+
Sbjct: 121 PSIAFLAVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGILKRESVPGKHVVRLEVD 180
Query: 181 KILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 240
KILH+GKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTF+THTFFQNHG
Sbjct: 181 KILHVGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFSTHTFFQNHG 240
Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEKSNL 300
FLYVQLPTITTTDSEGFSEKF VTTL GKAGNKVEPTEDKETDGVSLETVKAAVMEKS L
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFQVTTLIGKAGNKVEPTEDKETDGVSLETVKAAVMEKSKL 300
Query: 301 VKELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYLT 360
VKELERSESNKEALLAAV+DL+KTNELALQLEAR+K KSKSKKDKVNFSENFFSQETYLT
Sbjct: 301 VKELERSESNKEALLAAVKDLEKTNELALQLEAREKLKSKSKKDKVNFSENFFSQETYLT 360
Query: 361 VSGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDL 420
VSGQLHLESYACALGNVYSCGPRFRADKIES KHAAEMWMVEVEIAFAQLE+AM CADDL
Sbjct: 361 VSGQLHLESYACALGNVYSCGPRFRADKIESPKHAAEMWMVEVEIAFAQLENAMTCADDL 420
Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQ 480
VKFLSKWVLEHCLDDMKFVQKRIDKTSI RLESFISSSFEK+SYTAAIEIL K TDQKSQ
Sbjct: 421 VKFLSKWVLEHCLDDMKFVQKRIDKTSIQRLESFISSSFEKISYTAAIEILGKATDQKSQ 480
Query: 481 HLKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPK 540
HLK L+FGAALTADHLSYLAD+IYKKPVI Y YPK TKPFNVRLNDDGKTVAAFDVVLPK
Sbjct: 481 HLKKLEFGAALTADHLSYLADEIYKKPVIFYGYPKETKPFNVRLNDDGKTVAAFDVVLPK 540
Query: 541 GGVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTT 600
GVVISGSQKEERID+LM RM+EMGLP+EQ+EWY+EI RHGTVKHSGFS+AFDRF+LFTT
Sbjct: 541 AGVVISGSQKEERIDILMKRMREMGLPREQHEWYMEIQRHGTVKHSGFSLAFDRFVLFTT 600
Query: 601 GLTDVKDVIPFPRSFGKAGN 618
GLTD+KDVIPFPRS G A N
Sbjct: 601 GLTDIKDVIPFPRSLGNASN 620
BLAST of Sgr019312 vs. NCBI nr
Match:
XP_038899280.1 (asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida])
HSP 1 Score: 1034.6 bits (2674), Expect = 3.4e-298
Identity = 532/620 (85.81%), Postives = 569/620 (91.77%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKE-PPP 60
MAA+ P VFTPVTFSKYS RVLLKT+L+++DGGL FVGQK+VIGGWVKSSKEV++E P
Sbjct: 1 MAAEHPTVFTPVTFSKYSNRVLLKTLLDTADGGLAFVGQKLVIGGWVKSSKEVIREAAPS 60
Query: 61 PAPSGERPAAEA--PKAKDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPL 120
PA + R A A PKAKDVSCIEIIQSRIP VRSLLK+LYGNN GEKLET V KPPL
Sbjct: 61 PADASVRAVANALPPKAKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLETAVPKPPL 120
Query: 121 PSIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVE 180
PS AFL VNDGSCVASLQVVVESSLHHP+ LMPTGTCILVEG+LK+V V GKHVV+L+VE
Sbjct: 121 PSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKQVPVPGKHVVRLEVE 180
Query: 181 KILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 240
KILH+GKVEQ+KYPLS+K++PMD LRKFSYFRPRTTTVASVMRI NALTFATHTFFQNH
Sbjct: 181 KILHVGKVEQQKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNHS 240
Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEKSNL 300
FLYVQLPTITTTDSEGFSEKFHVTTLAGK G KVEPTEDKE DGVSLETVKAA+ EKSNL
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLAGK-GKKVEPTEDKEHDGVSLETVKAAITEKSNL 300
Query: 301 VKELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYLT 360
VKELERSESNKEAL+AAVQDLKKTNELALQLEA++KSK+KS+KDKV NFF QETYLT
Sbjct: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAKEKSKTKSRKDKV-IPGNFFPQETYLT 360
Query: 361 VSGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDL 420
SGQLHLE+YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA+LE+AMNCADDL
Sbjct: 361 ASGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELENAMNCADDL 420
Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQ 480
VKFLSKWVL+HCLDDMKFVQKRIDKTSIHRLES IS SFEKVSY AAIEILEK TDQKSQ
Sbjct: 421 VKFLSKWVLDHCLDDMKFVQKRIDKTSIHRLESVISCSFEKVSYIAAIEILEKATDQKSQ 480
Query: 481 HLKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPK 540
HLK LKFGAALT DHLSYLAD I+KKPVIVY+YPK TKPFNVRLNDDGKTVAAFDVVLPK
Sbjct: 481 HLKKLKFGAALTVDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPK 540
Query: 541 GGVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTT 600
GGVVISGSQKEER D+LMTRMKEMGLP+EQYEWYLEI RHGTVKHSGFS+AFDRF+LFTT
Sbjct: 541 GGVVISGSQKEERFDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTT 600
Query: 601 GLTDVKDVIPFPRSFGKAGN 618
GLTD+KDVIPFPRSFGK N
Sbjct: 601 GLTDIKDVIPFPRSFGKVSN 618
BLAST of Sgr019312 vs. NCBI nr
Match:
XP_008458080.1 (PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo])
HSP 1 Score: 1030.0 bits (2662), Expect = 8.3e-297
Identity = 527/619 (85.14%), Postives = 572/619 (92.41%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKE-PPP 60
MAAQQP VF+P+TFSKYS RVLLKT+L+++DGGL FVGQK+V+GGWVKSSKEV+++ PP
Sbjct: 1 MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60
Query: 61 PAPSGERPAAEAPKA-KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLP 120
PAP+ P PK KDVSCIEIIQSRIP VRSLLK+LYGNN GEKL+TPVHKPPLP
Sbjct: 61 PAPADSLP----PKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120
Query: 121 SIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVEK 180
S AFL +NDGSCVASLQVVVESSLHHP+ L+PTGTCILVEGVLK+V V GKHVV+L+VEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
Query: 181 ILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGF 240
ILH+GKVEQEKYPLS+K++PMD LRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+ F
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEKSNLV 300
LYVQLPTITTTDSEGFSEKFHVTTLAGK G +VE TEDKE DG+SLETVKAA+ EKSNLV
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGK-GKRVESTEDKEPDGLSLETVKAAITEKSNLV 300
Query: 301 KELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYLTV 360
KELERSESN+EAL+AAVQDLKKTNELALQLEAR+KSKSKS+KDKV NF QETYLTV
Sbjct: 301 KELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFLPQETYLTV 360
Query: 361 SGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLV 420
SGQLHLE+YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA+L+DAMNCADDLV
Sbjct: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLV 420
Query: 421 KFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQH 480
KFLSKWVLEHC+DDMKFVQKRIDKTSIHRLES IS SFEKVSYTAAIEILEK TDQKSQH
Sbjct: 421 KFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQH 480
Query: 481 LKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKG 540
LK LKFGAALTADHLSYLAD I+KKPVIVY+YPK TKPFNVRLNDDGKTVAAFDVVLPKG
Sbjct: 481 LKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540
Query: 541 GVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTG 600
GVVISGSQKEER+D+LMTRMKEMGLP+EQYEWYLEI RHGTVKHSGFS+AFDRF+LFTTG
Sbjct: 541 GVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTG 600
Query: 601 LTDVKDVIPFPRSFGKAGN 618
LTD+KDVIPFPRSFGK N
Sbjct: 601 LTDIKDVIPFPRSFGKVIN 613
BLAST of Sgr019312 vs. NCBI nr
Match:
TYJ96325.1 (asparagine--tRNA ligase [Cucumis melo var. makuwa])
HSP 1 Score: 1028.5 bits (2658), Expect = 2.4e-296
Identity = 525/619 (84.81%), Postives = 572/619 (92.41%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKE-PPP 60
MAAQQP VF+P+TFSKYS RVLLKT+L+++DGGL FVGQK+V+GGWVKSSKEV+++ PP
Sbjct: 1 MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60
Query: 61 PAPSGERPAAEAPKA-KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLP 120
PAP+ + PK KDVSCIEIIQSRIP VRSLLK+LYGNN GEKL+TPVHKPPLP
Sbjct: 61 PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120
Query: 121 SIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVEK 180
S AFL +NDGSCVASLQVVVESSLHHP+ L+PTGTCILVEGVLK+V V GKHVV+L+VEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
Query: 181 ILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGF 240
ILH+GKVEQEKYPLS+K++PMD LRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+ F
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEKSNLV 300
LYVQLPTITTTDSEGFSEKFHVTTLAGK G +VE TEDKE DG+SLETVKAA+ EKSNLV
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGK-GKRVESTEDKEPDGLSLETVKAAITEKSNLV 300
Query: 301 KELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYLTV 360
KELERSESN+EAL+AAVQDLKKTN+LALQLEAR+KSKSKS+KDKV NF QETYLTV
Sbjct: 301 KELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKV-IPGNFLPQETYLTV 360
Query: 361 SGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLV 420
SGQLHLE+YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA+L+DAMNCADDLV
Sbjct: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLV 420
Query: 421 KFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQH 480
KFLSKWVLEHC+DDMKFVQKRIDKTSIHRLES IS SFEKVSYTAAIEILEK TDQKSQH
Sbjct: 421 KFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQH 480
Query: 481 LKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKG 540
LK LKFGAALTADHLSYLAD I+KKPVIVY+YPK TKPFNVRLNDDGKTVAAFDVVLPKG
Sbjct: 481 LKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540
Query: 541 GVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTG 600
GVVISGSQKEER+D+LMTRMKEMGLP+EQYEWYLEI RHGTVKHSGFS+AFDRF+LFTTG
Sbjct: 541 GVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTG 600
Query: 601 LTDVKDVIPFPRSFGKAGN 618
LTD+KDVIPFPRSFGK N
Sbjct: 601 LTDIKDVIPFPRSFGKVIN 617
BLAST of Sgr019312 vs. NCBI nr
Match:
XP_004149331.1 (asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus])
HSP 1 Score: 1021.5 bits (2640), Expect = 2.9e-294
Identity = 522/619 (84.33%), Postives = 567/619 (91.60%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKE-PPP 60
MAAQQP VFTP+TFSKYS RVLLKT+L+++DGGL FVGQK V+GGWVKSSKEV+++ PP
Sbjct: 1 MAAQQPTVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
Query: 61 PAPSGERPAAEAPKA-KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLP 120
PAP+ P PK KDVSCIEIIQSRIP VRSLLK+LYGN+ DGEKLETPVHKPPLP
Sbjct: 61 PAPADSLP----PKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLP 120
Query: 121 SIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVEK 180
S AFL +NDGSCVASLQVVVESSLHHP+ L+PTGTCILVEGVLK+V V GKHVV+L+VEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
Query: 181 ILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGF 240
ILH+GKVEQEKYPLS+K++PMD LRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+ F
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEKSNLV 300
LYVQLPTITTTDSEGFSEKFHVTTLAGK G +VEPTEDKE DG+SLETVKAA+ EKSNLV
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGK-GKRVEPTEDKEPDGLSLETVKAAITEKSNLV 300
Query: 301 KELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYLTV 360
KELERSESN+EAL+ AVQDLKKTNELALQLEAR+KSKSKS+KDKV NFF QETYLTV
Sbjct: 301 KELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFFPQETYLTV 360
Query: 361 SGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLV 420
SGQLHLE+YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA+L+DAMNCADDLV
Sbjct: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLV 420
Query: 421 KFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQH 480
KFLSKWVLEHC+DDMKFVQKRIDK SIHRLES IS SFEKVSYT AIEILEK T+QKS H
Sbjct: 421 KFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPH 480
Query: 481 LKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKG 540
LK LKFGAALT DHLSYLAD I+KKPVIV +YPK TKPFNVRLNDDGKTVAAFDVVLPKG
Sbjct: 481 LKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540
Query: 541 GVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTG 600
GVVISGSQKEER+D+LMTRM+EMGLP+EQYEWYLEI RHG VKHSGFS+AFDRF+LFTTG
Sbjct: 541 GVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTG 600
Query: 601 LTDVKDVIPFPRSFGKAGN 618
LTD+KDVIPFPRSFGK N
Sbjct: 601 LTDIKDVIPFPRSFGKVSN 613
BLAST of Sgr019312 vs. ExPASy Swiss-Prot
Match:
Q9SW95 (Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 PE=1 SV=2)
HSP 1 Score: 621.3 bits (1601), Expect = 1.2e-176
Identity = 333/623 (53.45%), Postives = 446/623 (71.59%), Query Frame = 0
Query: 11 PVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKEPPPP------APSG 70
P+T SKYSKRV LKT+L+ SD G G G+++VIGGWVKS++ V K PPP APS
Sbjct: 20 PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79
Query: 71 ERPAAEAPKAKDVSCIEIIQSRIPFVRSLLKILY--GNNYQDGEKLETPVHK--PPLPSI 130
+A ++ C EIIQS++ + +L G Y +K E K PP +
Sbjct: 80 SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEYV 139
Query: 131 AFLVVNDGSCVASLQVVVESSLH--HPSQLMPTGTCILVEGVLK-RVSVTGKHVVQLQVE 190
+ +++DGS ++SLQVVV+S+L +QLM GTCI+ EGVL+ ++ + KHV++L+ E
Sbjct: 140 FYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEAE 199
Query: 191 KILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 250
K+LH+G V+ EKYPLSKK++P+ +LR FS+FRPRTTTV SV R+ +ALT A+HTF Q HG
Sbjct: 200 KLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYHG 259
Query: 251 FLYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTED-KETDGVSLETVKAAVMEKSN 310
F YVQ+P ITTT GF E F VTTL GK +K E +E DG S++TVKA + EK+
Sbjct: 260 FQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVIKEKTR 319
Query: 311 LVKELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKS--KKDKVNFSENFFSQET 370
L+ L+RS+SN+E ++AAV DLKKTN+LA Q+E + KSK+ + K +K++FS++FF ++T
Sbjct: 320 LIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFGRDT 379
Query: 371 YLTVSGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCA 430
YLT SG+ HLESYA ALG VY+ GPRF ADKI++A+H AE W VE E+AFA+L+DAM+CA
Sbjct: 380 YLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAMDCA 439
Query: 431 DDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQ 490
D+ KFL K+VLE+ +DMKF+ KR+DKT RLE+ SSS + SYT I +L+K T
Sbjct: 440 DEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKATTT 499
Query: 491 KSQHLKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVV 550
K + ++G ALT +HLSYL D+IYK PVIV+ YPK K F VRLNDD KTVAAFD+V
Sbjct: 500 KFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAFDLV 559
Query: 551 LPKGGVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLL 610
+PK GVVI+GSQ EER ++L R+ E G +E++EWYL++ RHGTVKHSG S++ ++ LL
Sbjct: 560 VPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQMLL 619
Query: 611 FTTGLTDVKDVIPFPRSFGKAGN 618
+ TGL D+KD IPFPRS+GKA N
Sbjct: 620 YATGLPDIKDAIPFPRSWGKANN 638
BLAST of Sgr019312 vs. ExPASy Swiss-Prot
Match:
Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)
HSP 1 Score: 472.6 bits (1215), Expect = 6.7e-132
Identity = 262/611 (42.88%), Postives = 374/611 (61.21%), Query Frame = 0
Query: 15 SKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKEPPPPAPSGERPAAEAPKA 74
+++S RVL++TIL+ DGG G GQ + IGGWVKS ++ G+R
Sbjct: 27 AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD----------QGKR-------- 86
Query: 75 KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLPSIAFLVVNDGSCVASL 134
+ +FL VNDGSC A+L
Sbjct: 87 --------------------------------------------TFSFLAVNDGSCPANL 146
Query: 135 QVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGK---HVVQLQVEKILHIGKVEQEKYP 194
QV+V+ SL+ S L+ TGTC+ V+GVLK V GK ++L V K++ +G V+ KYP
Sbjct: 147 QVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASKYP 206
Query: 195 LSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGFLYVQLPTITTTDS 254
L K ++ ++ LR + R RT ++++V RIRNAL FATH+FFQ H FLY+ P ITT+D
Sbjct: 207 LPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 266
Query: 255 EGFSEKFHVTTLAGKAGNKVEPTEDKETDG-----VSLETVKAAVMEKSNLVKELERSES 314
EG E F TTL N E E D +E + V+E+ N+V EL+ +++
Sbjct: 267 EGAGEMFQATTLI----NYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKA 326
Query: 315 NKEALLAAVQDLKKTNELALQLEARDKSK-SKSKKD-KVNFSENFFSQETYLTVSGQLHL 374
+KEA+ AAV +LK E ++ R + + KKD +++S++FF ++ +LTVSGQL +
Sbjct: 327 SKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQV 386
Query: 375 ESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLVKFLSKW 434
E+YACAL NVY+ GP FRA+ +++H AE WMVE EIAFA LED MNCA+ VK++ W
Sbjct: 387 ETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNW 446
Query: 435 VLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQHLKNLKF 494
+LE C DM+ + K D I RL+ S+ F +++YT AIE+LE+ + + N+++
Sbjct: 447 LLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEW 506
Query: 495 GAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 554
G L ++H YL + +++KP+IVY+YPKG K F +RLNDD KTVAA DV++PK G +I G
Sbjct: 507 GIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGG 566
Query: 555 SQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTGLTDVKD 614
SQ+EER D++ R++EMGLP E YEWYL++ R+GTVKH GF + F+R +LF TGL +++D
Sbjct: 567 SQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRD 570
Query: 615 VIPFPRSFGKA 616
VIPFPR GKA
Sbjct: 627 VIPFPRYPGKA 570
BLAST of Sgr019312 vs. ExPASy Swiss-Prot
Match:
Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)
HSP 1 Score: 434.1 bits (1115), Expect = 2.6e-120
Identity = 251/613 (40.95%), Postives = 361/613 (58.89%), Query Frame = 0
Query: 15 SKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKEPPPPAPSGERPAAEAPKA 74
+++S RVL+++IL GG GQK+ IGGWVK+ ++ K
Sbjct: 27 AQFSDRVLIRSIL---GGGAKLAGQKVRIGGWVKTGRQQGK------------------- 86
Query: 75 KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLPSIAFLVVNDGSCVASL 134
+ AFL VNDGSC A+L
Sbjct: 87 -------------------------------------------GTFAFLEVNDGSCPANL 146
Query: 135 QVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHV---VQLQVEKILHIGKVEQEKYP 194
QV+V+SSL+ S+L+ TGTC+ V+GVLK + GK + ++L VE ++ +G V+ YP
Sbjct: 147 QVMVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPTTYP 206
Query: 195 LSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGFLYVQLPTITTTDS 254
L K ++ + LR + R RT +++V RIRNAL FATH+FFQ H FLY+ P ITT+D
Sbjct: 207 LPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 266
Query: 255 EGFSEKFHVTTLAGKAGNKVEPTEDKETDG-----VSLETVKAAVMEKSNLVKELERSES 314
EG E F VTTL N E E D +E + V E+ V +L+ +++
Sbjct: 267 EGAGEMFQVTTLI----NHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKA 326
Query: 315 NKEALLAAVQDLKKTNELALQLEARDKSKSKSKKD--KVNFSENFFSQETYLTVSGQLHL 374
+KE + A+V L +E R + K K+ K+++S +FF ++ +LTVSGQL +
Sbjct: 327 SKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQV 386
Query: 375 ESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLVKFLSKW 434
E+YACAL +VY+ GP FRA+ +++H AE WMVE EIAFA + D MNCA+ VK++ KW
Sbjct: 387 ETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKW 446
Query: 435 VLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQHLKN-LK 494
+++ C DDM+ + K +D+ RL +SF++V+YT AIE LEK Q N ++
Sbjct: 447 LMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVE 506
Query: 495 FGAALTADHLSYLAD-DIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKGGVVI 554
+G L ++H YL + + +KP+IVY+YPKG K F +RLNDD KTVAA DV++PK G +I
Sbjct: 507 WGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELI 566
Query: 555 SGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTGLTDV 614
GSQ+EER D++ R++EMGLP E YEWYL++ R+GTVKH GF + F+R + F TG+ ++
Sbjct: 567 GGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNI 569
Query: 615 KDVIPFPRSFGKA 616
+DVIPFPR GKA
Sbjct: 627 RDVIPFPRYPGKA 569
BLAST of Sgr019312 vs. ExPASy Swiss-Prot
Match:
Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)
HSP 1 Score: 357.5 bits (916), Expect = 3.1e-97
Identity = 199/501 (39.72%), Postives = 292/501 (58.28%), Query Frame = 0
Query: 119 SIAFLVVNDGSCVASLQVVVESSLHHPSQL---MPTGTCILVEGVLKRVSVTGKHV-VQL 178
+ F+ +NDGS +++ Q++ + QL + TG + G + V GK +++
Sbjct: 37 TFTFIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVSVSAIGTI--VESPGKEQNLEM 96
Query: 179 QVEKILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQ 238
Q I IGK + E Y L KKR + LR ++ RPRT T+ +V R+RNAL FATH FFQ
Sbjct: 97 QATAITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQ 156
Query: 239 NHGFLYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEK 298
GFLY+ P IT +D EG + F VTTL
Sbjct: 157 KRGFLYIHTPIITGSDCEGAGKMFQVTTL------------------------------- 216
Query: 299 SNLVKELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQET 358
+ N A ++ + +V+++++FF + T
Sbjct: 217 ----------DQNNPA--------------------------RTPEGRVDYTQDFFGKPT 276
Query: 359 YLTVSGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCA 418
YLTVSGQL+ E YACAL +VY+ GP FRA+ +++H AE WM+E E+AFA L D M+CA
Sbjct: 277 YLTVSGQLNGEIYACALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCA 336
Query: 419 DDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQ 478
+D +K++ K+VL++C +DM+F K + I RLE I++SFE+ SYT A+ ILEK D+
Sbjct: 337 EDYLKYILKYVLDNCQEDMEFFNKHVATDLISRLEHVINTSFERASYTYAVRILEK-ADK 396
Query: 479 KSQHLKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVV 538
K ++ +K+G L ++H +LA++ + KPVI+ DYPK K F +R N+D KTVAA DV+
Sbjct: 397 KFEY--PVKWGLDLQSEHERFLAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMDVL 456
Query: 539 LPKGGVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLL 598
+PK G +I GSQ+EER+ +L +++KE LP E+Y WYLE+ + G+V HSGF F+R +
Sbjct: 457 VPKVGEIIGGSQREERLSVLESKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQ 465
Query: 599 FTTGLTDVKDVIPFPRSFGKA 616
FTTG+ +++DVIPFPR GKA
Sbjct: 517 FTTGMENIRDVIPFPRHPGKA 465
BLAST of Sgr019312 vs. ExPASy Swiss-Prot
Match:
P52276 (Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=asnS PE=3 SV=2)
HSP 1 Score: 346.3 bits (887), Expect = 7.2e-94
Identity = 196/501 (39.12%), Postives = 287/501 (57.29%), Query Frame = 0
Query: 114 KPPLPSIAFLVVNDGSCVASLQVVVESSLHHPSQL---MPTGTCILVEGVLKRVSVTGKH 173
K L +F+ VNDGS +A+LQVV++ SL +L + TG ++VEG L G+
Sbjct: 28 KRTLKDFSFVEVNDGSSLANLQVVLDGSLADYDRLLSQLQTGAALVVEGKLAPSPGKGQR 87
Query: 174 VVQLQVEKILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATH 233
V+L+ K+ +G + YPL KKR + LR + RPRT T+ +VMR+RNA A H
Sbjct: 88 -VELKATKLELLGGADPGSYPLQKKRHSFEFLRTIGHLRPRTNTIGAVMRVRNACATAIH 147
Query: 234 TFFQNHGFLYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAA 293
FFQ GFL+V P IT +D EG + F VTTL
Sbjct: 148 QFFQERGFLWVHTPIITASDCEGAGDLFKVTTL--------------------------- 207
Query: 294 VMEKSNLVKELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFF 353
+L+R N E +++S++FF
Sbjct: 208 ---------DLQRVPKNGEG--------------------------------IDYSQDFF 267
Query: 354 SQETYLTVSGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDA 413
++ YLTVSGQL E A A NVY+ GP FRA+ +++H AE WMVE E+AF LE
Sbjct: 268 GKQAYLTVSGQLEAEVMALAFQNVYTFGPTFRAENSNTSRHLAEFWMVEPEMAFCDLEGD 327
Query: 414 MNCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEK 473
A++ +K++ K+VLE C +DM+F +RID T + ++ I++ F ++Y+ AI++LEK
Sbjct: 328 RQWAEEFLKYIFKFVLEKCPEDMEFFDQRIDNTVLATADNIINNEFAWLTYSEAIKLLEK 387
Query: 474 TTDQKSQHLKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAA 533
DQK ++ + +G L ++H YLA+ ++K+P IV DYPK K F +RLNDDGKTVAA
Sbjct: 388 -ADQKFEY--PVAWGVDLQSEHERYLAETVFKRPTIVTDYPKDIKAFYMRLNDDGKTVAA 447
Query: 534 FDVVLPKGGVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFD 593
D++ PK G +I GSQ+EER+D+L RM+E G+P+E WYL++ R+G+V H+GF + F+
Sbjct: 448 MDILAPKIGEIIGGSQREERLDILTQRMQEQGVPEEDLWWYLDLRRYGSVPHAGFGLGFE 456
Query: 594 RFLLFTTGLTDVKDVIPFPRS 612
R + F TG+ +++DVIPFPR+
Sbjct: 508 RIVQFMTGMANIRDVIPFPRT 456
BLAST of Sgr019312 vs. ExPASy TrEMBL
Match:
A0A6J1DR84 (Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111023596 PE=3 SV=1)
HSP 1 Score: 1068.9 bits (2763), Expect = 7.8e-309
Identity = 544/620 (87.74%), Postives = 578/620 (93.23%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKE---P 60
MAAQQPA FTP FSKYSKRVLLK++LES+DGGLGFV QK+V+GGWVKSSKEVV++ P
Sbjct: 1 MAAQQPAAFTPAAFSKYSKRVLLKSVLESADGGLGFVDQKLVVGGWVKSSKEVVRDTARP 60
Query: 61 PPPAPSGERPAAEAPKAKDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPL 120
PP + + P+AKDVSCIEIIQSRIP VRSLL+ILYGN++ GEKLETPVHKPPL
Sbjct: 61 PPDSGAAVEALPPPPRAKDVSCIEIIQSRIPIVRSLLRILYGNSFHAGEKLETPVHKPPL 120
Query: 121 PSIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVE 180
PSIAFL VNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEG+LKR SV GKHVV+L+V+
Sbjct: 121 PSIAFLAVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGILKRESVPGKHVVRLEVD 180
Query: 181 KILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 240
KILH+GKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTF+THTFFQNHG
Sbjct: 181 KILHVGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFSTHTFFQNHG 240
Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEKSNL 300
FLYVQLPTITTTDSEGFSEKF VTTL GKAGNKVEPTEDKETDGVSLETVKAAVMEKS L
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFQVTTLIGKAGNKVEPTEDKETDGVSLETVKAAVMEKSKL 300
Query: 301 VKELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYLT 360
VKELERSESNKEALLAAV+DL+KTNELALQLEAR+K KSKSKKDKVNFSENFFSQETYLT
Sbjct: 301 VKELERSESNKEALLAAVKDLEKTNELALQLEAREKLKSKSKKDKVNFSENFFSQETYLT 360
Query: 361 VSGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDL 420
VSGQLHLESYACALGNVYSCGPRFRADKIES KHAAEMWMVEVEIAFAQLE+AM CADDL
Sbjct: 361 VSGQLHLESYACALGNVYSCGPRFRADKIESPKHAAEMWMVEVEIAFAQLENAMTCADDL 420
Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQ 480
VKFLSKWVLEHCLDDMKFVQKRIDKTSI RLESFISSSFEK+SYTAAIEIL K TDQKSQ
Sbjct: 421 VKFLSKWVLEHCLDDMKFVQKRIDKTSIQRLESFISSSFEKISYTAAIEILGKATDQKSQ 480
Query: 481 HLKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPK 540
HLK L+FGAALTADHLSYLAD+IYKKPVI Y YPK TKPFNVRLNDDGKTVAAFDVVLPK
Sbjct: 481 HLKKLEFGAALTADHLSYLADEIYKKPVIFYGYPKETKPFNVRLNDDGKTVAAFDVVLPK 540
Query: 541 GGVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTT 600
GVVISGSQKEERID+LM RM+EMGLP+EQ+EWY+EI RHGTVKHSGFS+AFDRF+LFTT
Sbjct: 541 AGVVISGSQKEERIDILMKRMREMGLPREQHEWYMEIQRHGTVKHSGFSLAFDRFVLFTT 600
Query: 601 GLTDVKDVIPFPRSFGKAGN 618
GLTD+KDVIPFPRS G A N
Sbjct: 601 GLTDIKDVIPFPRSLGNASN 620
BLAST of Sgr019312 vs. ExPASy TrEMBL
Match:
A0A1S3C6Z5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1)
HSP 1 Score: 1030.0 bits (2662), Expect = 4.0e-297
Identity = 527/619 (85.14%), Postives = 572/619 (92.41%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKE-PPP 60
MAAQQP VF+P+TFSKYS RVLLKT+L+++DGGL FVGQK+V+GGWVKSSKEV+++ PP
Sbjct: 1 MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60
Query: 61 PAPSGERPAAEAPKA-KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLP 120
PAP+ P PK KDVSCIEIIQSRIP VRSLLK+LYGNN GEKL+TPVHKPPLP
Sbjct: 61 PAPADSLP----PKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120
Query: 121 SIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVEK 180
S AFL +NDGSCVASLQVVVESSLHHP+ L+PTGTCILVEGVLK+V V GKHVV+L+VEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
Query: 181 ILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGF 240
ILH+GKVEQEKYPLS+K++PMD LRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+ F
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEKSNLV 300
LYVQLPTITTTDSEGFSEKFHVTTLAGK G +VE TEDKE DG+SLETVKAA+ EKSNLV
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGK-GKRVESTEDKEPDGLSLETVKAAITEKSNLV 300
Query: 301 KELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYLTV 360
KELERSESN+EAL+AAVQDLKKTNELALQLEAR+KSKSKS+KDKV NF QETYLTV
Sbjct: 301 KELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFLPQETYLTV 360
Query: 361 SGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLV 420
SGQLHLE+YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA+L+DAMNCADDLV
Sbjct: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLV 420
Query: 421 KFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQH 480
KFLSKWVLEHC+DDMKFVQKRIDKTSIHRLES IS SFEKVSYTAAIEILEK TDQKSQH
Sbjct: 421 KFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQH 480
Query: 481 LKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKG 540
LK LKFGAALTADHLSYLAD I+KKPVIVY+YPK TKPFNVRLNDDGKTVAAFDVVLPKG
Sbjct: 481 LKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540
Query: 541 GVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTG 600
GVVISGSQKEER+D+LMTRMKEMGLP+EQYEWYLEI RHGTVKHSGFS+AFDRF+LFTTG
Sbjct: 541 GVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTG 600
Query: 601 LTDVKDVIPFPRSFGKAGN 618
LTD+KDVIPFPRSFGK N
Sbjct: 601 LTDIKDVIPFPRSFGKVIN 613
BLAST of Sgr019312 vs. ExPASy TrEMBL
Match:
A0A5D3BD07 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00300 PE=3 SV=1)
HSP 1 Score: 1028.5 bits (2658), Expect = 1.2e-296
Identity = 525/619 (84.81%), Postives = 572/619 (92.41%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKE-PPP 60
MAAQQP VF+P+TFSKYS RVLLKT+L+++DGGL FVGQK+V+GGWVKSSKEV+++ PP
Sbjct: 1 MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60
Query: 61 PAPSGERPAAEAPKA-KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLP 120
PAP+ + PK KDVSCIEIIQSRIP VRSLLK+LYGNN GEKL+TPVHKPPLP
Sbjct: 61 PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120
Query: 121 SIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVEK 180
S AFL +NDGSCVASLQVVVESSLHHP+ L+PTGTCILVEGVLK+V V GKHVV+L+VEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
Query: 181 ILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGF 240
ILH+GKVEQEKYPLS+K++PMD LRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+ F
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEKSNLV 300
LYVQLPTITTTDSEGFSEKFHVTTLAGK G +VE TEDKE DG+SLETVKAA+ EKSNLV
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGK-GKRVESTEDKEPDGLSLETVKAAITEKSNLV 300
Query: 301 KELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYLTV 360
KELERSESN+EAL+AAVQDLKKTN+LALQLEAR+KSKSKS+KDKV NF QETYLTV
Sbjct: 301 KELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKV-IPGNFLPQETYLTV 360
Query: 361 SGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLV 420
SGQLHLE+YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA+L+DAMNCADDLV
Sbjct: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLV 420
Query: 421 KFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQH 480
KFLSKWVLEHC+DDMKFVQKRIDKTSIHRLES IS SFEKVSYTAAIEILEK TDQKSQH
Sbjct: 421 KFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQH 480
Query: 481 LKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKG 540
LK LKFGAALTADHLSYLAD I+KKPVIVY+YPK TKPFNVRLNDDGKTVAAFDVVLPKG
Sbjct: 481 LKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540
Query: 541 GVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTG 600
GVVISGSQKEER+D+LMTRMKEMGLP+EQYEWYLEI RHGTVKHSGFS+AFDRF+LFTTG
Sbjct: 541 GVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTG 600
Query: 601 LTDVKDVIPFPRSFGKAGN 618
LTD+KDVIPFPRSFGK N
Sbjct: 601 LTDIKDVIPFPRSFGKVIN 617
BLAST of Sgr019312 vs. ExPASy TrEMBL
Match:
A0A0A0K8G1 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1)
HSP 1 Score: 1021.5 bits (2640), Expect = 1.4e-294
Identity = 522/619 (84.33%), Postives = 567/619 (91.60%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKE-PPP 60
MAAQQP VFTP+TFSKYS RVLLKT+L+++DGGL FVGQK V+GGWVKSSKEV+++ PP
Sbjct: 1 MAAQQPTVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
Query: 61 PAPSGERPAAEAPKA-KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLP 120
PAP+ P PK KDVSCIEIIQSRIP VRSLLK+LYGN+ DGEKLETPVHKPPLP
Sbjct: 61 PAPADSLP----PKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLP 120
Query: 121 SIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVEK 180
S AFL +NDGSCVASLQVVVESSLHHP+ L+PTGTCILVEGVLK+V V GKHVV+L+VEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
Query: 181 ILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGF 240
ILH+GKVEQEKYPLS+K++PMD LRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+ F
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEKSNLV 300
LYVQLPTITTTDSEGFSEKFHVTTLAGK G +VEPTEDKE DG+SLETVKAA+ EKSNLV
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGK-GKRVEPTEDKEPDGLSLETVKAAITEKSNLV 300
Query: 301 KELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYLTV 360
KELERSESN+EAL+ AVQDLKKTNELALQLEAR+KSKSKS+KDKV NFF QETYLTV
Sbjct: 301 KELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFFPQETYLTV 360
Query: 361 SGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLV 420
SGQLHLE+YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA+L+DAMNCADDLV
Sbjct: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLV 420
Query: 421 KFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQH 480
KFLSKWVLEHC+DDMKFVQKRIDK SIHRLES IS SFEKVSYT AIEILEK T+QKS H
Sbjct: 421 KFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPH 480
Query: 481 LKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKG 540
LK LKFGAALT DHLSYLAD I+KKPVIV +YPK TKPFNVRLNDDGKTVAAFDVVLPKG
Sbjct: 481 LKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540
Query: 541 GVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTG 600
GVVISGSQKEER+D+LMTRM+EMGLP+EQYEWYLEI RHG VKHSGFS+AFDRF+LFTTG
Sbjct: 541 GVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTG 600
Query: 601 LTDVKDVIPFPRSFGKAGN 618
LTD+KDVIPFPRSFGK N
Sbjct: 601 LTDIKDVIPFPRSFGKVSN 613
BLAST of Sgr019312 vs. ExPASy TrEMBL
Match:
A0A6J1GR04 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456649 PE=3 SV=1)
HSP 1 Score: 1018.1 bits (2631), Expect = 1.6e-293
Identity = 523/622 (84.08%), Postives = 564/622 (90.68%), Query Frame = 0
Query: 1 MAAQQPAVFTPVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKEPPPP 60
MAAQQP VFTPVTFSKY+KRVLL+T+ ES DGGLGFV +K+V+GGWVKSSKEV+ + P
Sbjct: 1 MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
Query: 61 APSGERPAAE---APKAKDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPL 120
AA KAKD SCIEI+QSRIP VRSLL++LYGNN GEKLET VH+PPL
Sbjct: 61 LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120
Query: 121 PSIAFLVVNDGSCVASLQVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHVVQLQVE 180
PS AFLVVNDGSCVA LQVVVESSLH P LMP+GTCILVEG+LKR+ V GKH V+L+VE
Sbjct: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180
Query: 181 KILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 240
KILH+GKVE+EKYPLS+KR+PMD+LRKFSYFRPRT+TVASVMRIRNALTFATHTFFQNH
Sbjct: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240
Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLAGK-AGNKVEPTEDKETDGVSLETVKAAVMEKSN 300
FLYVQLPTITTTDSEGFSEKFHVTTLA K AG +VEPTEDKETDGVSLETVKAAVMEKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300
Query: 301 LVKELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQETYL 360
LVKELERSE NKEAL AAVQDLK+TNELALQLEAR KSK +S KDKVNF ENFF QETYL
Sbjct: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360
Query: 361 TVSGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADD 420
TVSGQLHLE+YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA+LEDAMNCADD
Sbjct: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420
Query: 421 LVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTT-DQK 480
LVKFLSKWVL+HC+DDMKFV+KRIDKTSI RLESFIS SFEKVSYTAAIE LEK T DQK
Sbjct: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480
Query: 481 SQHLKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVL 540
QHLK+LKFGAALTADHLSYLAD IYKKPVIVY+YP+GTKPFNVRLNDDGKTVAAFDVVL
Sbjct: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540
Query: 541 PKGGVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLF 600
PKGGVVISGSQKEER+D+LM RM EMGLP+EQYEWYL+I RHG VKHSGFS+AFDRFLLF
Sbjct: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600
Query: 601 TTGLTDVKDVIPFPRSFGKAGN 618
TTGLTD+KDVIPFPRSFGKA +
Sbjct: 601 TTGLTDIKDVIPFPRSFGKASD 622
BLAST of Sgr019312 vs. TAIR 10
Match:
AT3G07420.1 (asparaginyl-tRNA synthetase 2 )
HSP 1 Score: 621.3 bits (1601), Expect = 8.3e-178
Identity = 333/623 (53.45%), Postives = 446/623 (71.59%), Query Frame = 0
Query: 11 PVTFSKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKEPPPP------APSG 70
P+T SKYSKRV LKT+L+ SD G G G+++VIGGWVKS++ V K PPP APS
Sbjct: 20 PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79
Query: 71 ERPAAEAPKAKDVSCIEIIQSRIPFVRSLLKILY--GNNYQDGEKLETPVHK--PPLPSI 130
+A ++ C EIIQS++ + +L G Y +K E K PP +
Sbjct: 80 SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEYV 139
Query: 131 AFLVVNDGSCVASLQVVVESSLH--HPSQLMPTGTCILVEGVLK-RVSVTGKHVVQLQVE 190
+ +++DGS ++SLQVVV+S+L +QLM GTCI+ EGVL+ ++ + KHV++L+ E
Sbjct: 140 FYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEAE 199
Query: 191 KILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 250
K+LH+G V+ EKYPLSKK++P+ +LR FS+FRPRTTTV SV R+ +ALT A+HTF Q HG
Sbjct: 200 KLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYHG 259
Query: 251 FLYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTED-KETDGVSLETVKAAVMEKSN 310
F YVQ+P ITTT GF E F VTTL GK +K E +E DG S++TVKA + EK+
Sbjct: 260 FQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVIKEKTR 319
Query: 311 LVKELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKS--KKDKVNFSENFFSQET 370
L+ L+RS+SN+E ++AAV DLKKTN+LA Q+E + KSK+ + K +K++FS++FF ++T
Sbjct: 320 LIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFGRDT 379
Query: 371 YLTVSGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCA 430
YLT SG+ HLESYA ALG VY+ GPRF ADKI++A+H AE W VE E+AFA+L+DAM+CA
Sbjct: 380 YLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAMDCA 439
Query: 431 DDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQ 490
D+ KFL K+VLE+ +DMKF+ KR+DKT RLE+ SSS + SYT I +L+K T
Sbjct: 440 DEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKATTT 499
Query: 491 KSQHLKNLKFGAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVV 550
K + ++G ALT +HLSYL D+IYK PVIV+ YPK K F VRLNDD KTVAAFD+V
Sbjct: 500 KFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAFDLV 559
Query: 551 LPKGGVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLL 610
+PK GVVI+GSQ EER ++L R+ E G +E++EWYL++ RHGTVKHSG S++ ++ LL
Sbjct: 560 VPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQMLL 619
Query: 611 FTTGLTDVKDVIPFPRSFGKAGN 618
+ TGL D+KD IPFPRS+GKA N
Sbjct: 620 YATGLPDIKDAIPFPRSWGKANN 638
BLAST of Sgr019312 vs. TAIR 10
Match:
AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )
HSP 1 Score: 472.6 bits (1215), Expect = 4.7e-133
Identity = 262/611 (42.88%), Postives = 374/611 (61.21%), Query Frame = 0
Query: 15 SKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKEPPPPAPSGERPAAEAPKA 74
+++S RVL++TIL+ DGG G GQ + IGGWVKS ++ G+R
Sbjct: 27 AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD----------QGKR-------- 86
Query: 75 KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLPSIAFLVVNDGSCVASL 134
+ +FL VNDGSC A+L
Sbjct: 87 --------------------------------------------TFSFLAVNDGSCPANL 146
Query: 135 QVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGK---HVVQLQVEKILHIGKVEQEKYP 194
QV+V+ SL+ S L+ TGTC+ V+GVLK V GK ++L V K++ +G V+ KYP
Sbjct: 147 QVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASKYP 206
Query: 195 LSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGFLYVQLPTITTTDS 254
L K ++ ++ LR + R RT ++++V RIRNAL FATH+FFQ H FLY+ P ITT+D
Sbjct: 207 LPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 266
Query: 255 EGFSEKFHVTTLAGKAGNKVEPTEDKETDG-----VSLETVKAAVMEKSNLVKELERSES 314
EG E F TTL N E E D +E + V+E+ N+V EL+ +++
Sbjct: 267 EGAGEMFQATTLI----NYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKA 326
Query: 315 NKEALLAAVQDLKKTNELALQLEARDKSK-SKSKKD-KVNFSENFFSQETYLTVSGQLHL 374
+KEA+ AAV +LK E ++ R + + KKD +++S++FF ++ +LTVSGQL +
Sbjct: 327 SKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQV 386
Query: 375 ESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLVKFLSKW 434
E+YACAL NVY+ GP FRA+ +++H AE WMVE EIAFA LED MNCA+ VK++ W
Sbjct: 387 ETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNW 446
Query: 435 VLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQHLKNLKF 494
+LE C DM+ + K D I RL+ S+ F +++YT AIE+LE+ + + N+++
Sbjct: 447 LLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEW 506
Query: 495 GAALTADHLSYLADDIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 554
G L ++H YL + +++KP+IVY+YPKG K F +RLNDD KTVAA DV++PK G +I G
Sbjct: 507 GIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGG 566
Query: 555 SQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTGLTDVKD 614
SQ+EER D++ R++EMGLP E YEWYL++ R+GTVKH GF + F+R +LF TGL +++D
Sbjct: 567 SQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRD 570
Query: 615 VIPFPRSFGKA 616
VIPFPR GKA
Sbjct: 627 VIPFPRYPGKA 570
BLAST of Sgr019312 vs. TAIR 10
Match:
AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )
HSP 1 Score: 434.1 bits (1115), Expect = 1.9e-121
Identity = 251/613 (40.95%), Postives = 361/613 (58.89%), Query Frame = 0
Query: 15 SKYSKRVLLKTILESSDGGLGFVGQKMVIGGWVKSSKEVVKEPPPPAPSGERPAAEAPKA 74
+++S RVL+++IL GG GQK+ IGGWVK+ ++ K
Sbjct: 27 AQFSDRVLIRSIL---GGGAKLAGQKVRIGGWVKTGRQQGK------------------- 86
Query: 75 KDVSCIEIIQSRIPFVRSLLKILYGNNYQDGEKLETPVHKPPLPSIAFLVVNDGSCVASL 134
+ AFL VNDGSC A+L
Sbjct: 87 -------------------------------------------GTFAFLEVNDGSCPANL 146
Query: 135 QVVVESSLHHPSQLMPTGTCILVEGVLKRVSVTGKHV---VQLQVEKILHIGKVEQEKYP 194
QV+V+SSL+ S+L+ TGTC+ V+GVLK + GK + ++L VE ++ +G V+ YP
Sbjct: 147 QVMVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPTTYP 206
Query: 195 LSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGFLYVQLPTITTTDS 254
L K ++ + LR + R RT +++V RIRNAL FATH+FFQ H FLY+ P ITT+D
Sbjct: 207 LPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 266
Query: 255 EGFSEKFHVTTLAGKAGNKVEPTEDKETDG-----VSLETVKAAVMEKSNLVKELERSES 314
EG E F VTTL N E E D +E + V E+ V +L+ +++
Sbjct: 267 EGAGEMFQVTTLI----NHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKA 326
Query: 315 NKEALLAAVQDLKKTNELALQLEARDKSKSKSKKD--KVNFSENFFSQETYLTVSGQLHL 374
+KE + A+V L +E R + K K+ K+++S +FF ++ +LTVSGQL +
Sbjct: 327 SKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQV 386
Query: 375 ESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLVKFLSKW 434
E+YACAL +VY+ GP FRA+ +++H AE WMVE EIAFA + D MNCA+ VK++ KW
Sbjct: 387 ETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKW 446
Query: 435 VLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQKSQHLKN-LK 494
+++ C DDM+ + K +D+ RL +SF++V+YT AIE LEK Q N ++
Sbjct: 447 LMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVE 506
Query: 495 FGAALTADHLSYLAD-DIYKKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDVVLPKGGVVI 554
+G L ++H YL + + +KP+IVY+YPKG K F +RLNDD KTVAA DV++PK G +I
Sbjct: 507 WGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELI 566
Query: 555 SGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFLLFTTGLTDV 614
GSQ+EER D++ R++EMGLP E YEWYL++ R+GTVKH GF + F+R + F TG+ ++
Sbjct: 567 GGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNI 569
Query: 615 KDVIPFPRSFGKA 616
+DVIPFPR GKA
Sbjct: 627 RDVIPFPRYPGKA 569
BLAST of Sgr019312 vs. TAIR 10
Match:
AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )
HSP 1 Score: 330.1 bits (845), Expect = 3.8e-90
Identity = 189/498 (37.95%), Postives = 291/498 (58.43%), Query Frame = 0
Query: 119 SIAFLVVNDGSCVASLQVVVESSLHHPSQL----MPTGTCILVEGVLKRVSVTGKHVVQL 178
S+ F+ +NDGSC+++LQ V+ S Q+ + TG + V+G + T K V+L
Sbjct: 126 SVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVASQGT-KQKVEL 185
Query: 179 QVEKILHIGKVEQEKYPLSKKRMPMDILRKFSYFRPRTTTVASVMRIRNALTFATHTFFQ 238
+VEKI+ +G+ + YP+ KKR+ + LR ++ RPRT T +V R+RN L +ATH FFQ
Sbjct: 186 KVEKIIVVGECD-SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQ 245
Query: 239 NHGFLYVQLPTITTTDSEGFSEKFHVTTLAGKAGNKVEPTEDKETDGVSLETVKAAVMEK 298
GF++V P IT +D EG E+F VTTL P+ + TD S++ +
Sbjct: 246 ESGFVWVASPIITASDCEGAGEQFCVTTLI--------PSSHENTD-TSIDAI------- 305
Query: 299 SNLVKELERSESNKEALLAAVQDLKKTNELALQLEARDKSKSKSKKDKVNFSENFFSQET 358
K+K +++S++FF +
Sbjct: 306 -----------------------------------------PKTKGGLIDWSQDFFGKPA 365
Query: 359 YLTVSGQLHLESYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCA 418
+LTVSGQL+ E+YA AL +VY+ GP FRA+ +++H AE WM+E E+AFA L+D M CA
Sbjct: 366 FLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACA 425
Query: 419 DDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFISSSFEKVSYTAAIEILEKTTDQ 478
++++ K+VL++C +DM+F I+K I RL F ++ YT AIEIL K +
Sbjct: 426 TAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKK 485
Query: 479 KSQHLKNLKFGAALTADHLSYLADDIY-KKPVIVYDYPKGTKPFNVRLNDDGKTVAAFDV 538
+K+G L ++H Y+ ++ + +PVI+ DYPK K F +R NDDGKTVAA D+
Sbjct: 486 FD---FPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDM 545
Query: 539 VLPKGGVVISGSQKEERIDLLMTRMKEMGLPKEQYEWYLEILRHGTVKHSGFSVAFDRFL 598
++P+ G +I GSQ+EER+++L R+ E+ L KE Y WYL++ R+G+V H+GF + F+R +
Sbjct: 546 LVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLV 561
Query: 599 LFTTGLTDVKDVIPFPRS 612
F TG+ +++DVIPFPR+
Sbjct: 606 QFVTGIDNIRDVIPFPRT 561
BLAST of Sgr019312 vs. TAIR 10
Match:
AT1G68420.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 83.6 bits (205), Expect = 6.2e-16
Identity = 36/77 (46.75%), Postives = 58/77 (75.32%), Query Frame = 0
Query: 371 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAQLEDAMNCADDLVKFLSKWVLEHCL 430
+G+VY+ P FRA+K +++H AE WMVEVE+AFA +E+AMNC++ +VK + +LE C
Sbjct: 1 MGDVYTFAPTFRAEKSHTSRHLAEFWMVEVELAFAGVEEAMNCSEAVVKDMCTTLLEKCR 60
Query: 431 DDMKFVQKRIDKTSIHR 448
DDM+++ +++D+ I R
Sbjct: 61 DDMEYMVEKVDEFCIDR 77
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022156755.1 | 1.6e-308 | 87.74 | asparagine--tRNA ligase, cytoplasmic 2 [Momordica charantia] | [more] |
| XP_038899280.1 | 3.4e-298 | 85.81 | asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida] | [more] |
| XP_008458080.1 | 8.3e-297 | 85.14 | PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo] | [more] |
| TYJ96325.1 | 2.4e-296 | 84.81 | asparagine--tRNA ligase [Cucumis melo var. makuwa] | [more] |
| XP_004149331.1 | 2.9e-294 | 84.33 | asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9SW95 | 1.2e-176 | 53.45 | Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 ... | [more] |
| Q9SW96 | 6.7e-132 | 42.88 | Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ... | [more] |
| Q9SSK1 | 2.6e-120 | 40.95 | Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ... | [more] |
| Q6MEC9 | 3.1e-97 | 39.72 | Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... | [more] |
| P52276 | 7.2e-94 | 39.12 | Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DR84 | 7.8e-309 | 87.74 | Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111023596 PE=3 SV=1 | [more] |
| A0A1S3C6Z5 | 4.0e-297 | 85.14 | Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1 | [more] |
| A0A5D3BD07 | 1.2e-296 | 84.81 | Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... | [more] |
| A0A0A0K8G1 | 1.4e-294 | 84.33 | Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1 | [more] |
| A0A6J1GR04 | 1.6e-293 | 84.08 | Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456649 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G07420.1 | 8.3e-178 | 53.45 | asparaginyl-tRNA synthetase 2 | [more] |
| AT5G56680.1 | 4.7e-133 | 42.88 | Class II aminoacyl-tRNA and biotin synthetases superfamily protein | [more] |
| AT1G70980.1 | 1.9e-121 | 40.95 | Class II aminoacyl-tRNA and biotin synthetases superfamily protein | [more] |
| AT4G17300.1 | 3.8e-90 | 37.95 | Class II aminoacyl-tRNA and biotin synthetases superfamily protein | [more] |
| AT1G68420.1 | 6.2e-16 | 46.75 | Class II aaRS and biotin synthetases superfamily protein | [more] |
