Sgr019193 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr019193 vs. NCBI nr
Match: XP_022142258.1 (MACPF domain-containing protein CAD1 [Momordica charantia])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 546/575 (94.96%), Postives = 558/575 (97.04%), Query Frame = 0

Query: 1   MKDPCATQTAA---VTATSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQ 60
           MKDP A+QT A    T+TS+MDALT TL NSIQALGRGFDVTADIRLLYCKGTPGS L+Q
Sbjct: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60

Query: 61  LDDAHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPL 120
           LDDAHTRDL+LTDGV+VPNVP+DIQCSSDNRATENVPVC+FHKMAEYFN KSGISGNIPL
Sbjct: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFNAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQVDAAATKSLAMVGY IPLFKVEL NSNL L EDIKRAVPYTWDPVS
Sbjct: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHL 300
           GPLSYKDKDVTVIFRRRGGDDLVQSHA WA+TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKP TGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSTPATPLRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPS PATPLRSDGASSSSK+RTSEDKKEDSSSNAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575

BLAST of Sgr019193 vs. NCBI nr
Match: XP_038883129.1 (MACPF domain-containing protein CAD1 [Benincasa hispida])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 539/572 (94.23%), Postives = 550/572 (96.15%), Query Frame = 0

Query: 1   MKDPCATQTAAVTATSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLDD 60
           MKDP  TQTAA  ATS MDALTTTL NSI ALGRGFDVTADIRLLYCKGTPGS LV LDD
Sbjct: 1   MKDPRTTQTAAAIATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVHLDD 60

Query: 61  AHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGSF 120
           A TRDL+L+DGV+VPNVP+D+QCSSD+RATENVPVCSFHKMAEYFN KS ISGNIPLGSF
Sbjct: 61  ARTRDLVLSDGVVVPNVPDDVQCSSDSRATENVPVCSFHKMAEYFNQKSDISGNIPLGSF 120

Query: 121 NAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLAS 180
           NAMFNFSGSWQVDAAATKSLAM+GY+IPLFKV L NSNL L EDIKRAVPYTWDPVSLAS
Sbjct: 121 NAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKNSNLVLCEDIKRAVPYTWDPVSLAS 180

Query: 181 FIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGPL 240
           FIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQST  PL
Sbjct: 181 FIENFGTHIITSATIGGRDVVYIRQHQSSPLSELEIEHYVKEVGEQRFLDSKSQSTASPL 240

Query: 241 SYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLARA 300
           SYKDKDVTVIFRRRGGDDLVQSHA WA+TVKSAPDVINMTFTPIVSMLEGVPGVKHLARA
Sbjct: 241 SYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLARA 300

Query: 301 IDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQIT 360
           IDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQIT
Sbjct: 301 IDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQIT 360

Query: 361 VGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRW 420
           VGRKP TGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRW
Sbjct: 361 VGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRW 420

Query: 421 FEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPG 480
           FEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPG
Sbjct: 421 FEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPG 480

Query: 481 CTIRRSVWDHSPSTPATPLRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMSKGP 540
           CTIRRSVWDHSPSTPATPLRSDGASSSSK+R SEDKKEDSS+NAGKLAKIVDLTEMSKGP
Sbjct: 481 CTIRRSVWDHSPSTPATPLRSDGASSSSKTRISEDKKEDSSTNAGKLAKIVDLTEMSKGP 540

Query: 541 QDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           QDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 QDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 572

BLAST of Sgr019193 vs. NCBI nr
Match: XP_008439705.1 (PREDICTED: MACPF domain-containing protein CAD1 [Cucumis melo] >TYK13232.1 MACPF domain-containing protein CAD1 [Cucumis melo var. makuwa])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 539/573 (94.07%), Postives = 553/573 (96.51%), Query Frame = 0

Query: 1   MKDPCAT-QTAAVTATSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLD 60
           MKDP AT QTAA TATS MDALTTTL NSI ALGRGFDVTADIRLLYCKGTPGS LVQLD
Sbjct: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGS 120
           DAHTRDL+L+DGV+VPNVP+D+QCSSD RATENVPVCSFHKMAEYFN KSGISGNIPLGS
Sbjct: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQ+DAAATKSLAM+GY+IP FKV L +SNL L EDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQS   P
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHA WA+TVKSAPDVINMTFTPIVSMLEGVPGVKHLAR
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKP TGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMSKG 540
           GCTIRRSVWDHSPSTPATPLRSDGASSSS++RTSEDKKEDSS+NAGKLAKIVDLTEMSKG
Sbjct: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           PQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 573

BLAST of Sgr019193 vs. NCBI nr
Match: XP_022926839.1 (MACPF domain-containing protein CAD1 [Cucurbita moschata])

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 535/573 (93.37%), Postives = 546/573 (95.29%), Query Frame = 0

Query: 1   MKDPCATQTAAVTA-TSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLD 60
           MKDPC T  AA T  TS MDALT TL NSIQALGRGFDVTADIRLLYCKGTPGS LVQLD
Sbjct: 1   MKDPCTTYAAAATTPTSEMDALTATLCNSIQALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGS 120
           DA TRDL L+DGV+VPNVP+ + CSSD RATENVPVCSFHKMAEYFN KS +SGNIPLGS
Sbjct: 61  DASTRDLHLSDGVVVPNVPDHVHCSSDTRATENVPVCSFHKMAEYFNQKSNLSGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQVDAAATKSLAM+GYFIPLFKVEL NS L LHE+IKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQVDAAATKSLAMIGYFIPLFKVELKNSTLVLHEEIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIE+YVKEVGEQRFLDSKSQSTVGP
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIENYVKEVGEQRFLDSKSQSTVGP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHA WAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKP TGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGA KWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGALKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMSKG 540
           GCT+RRSVWDHSPST ATP RSDGASSSSK++T EDKKEDSSSNAGKLAKIVDLTEMSKG
Sbjct: 481 GCTVRRSVWDHSPSTSATPSRSDGASSSSKTKTGEDKKEDSSSNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573

BLAST of Sgr019193 vs. NCBI nr
Match: XP_011658263.1 (MACPF domain-containing protein CAD1 [Cucumis sativus] >KAE8647726.1 hypothetical protein Csa_003370 [Cucumis sativus])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 534/573 (93.19%), Postives = 549/573 (95.81%), Query Frame = 0

Query: 1   MKDPCAT-QTAAVTATSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLD 60
           MKDP AT QT+A T TS MDALTTTL NSI ALGRGFDVTADIRLLYCKGTPGS LVQLD
Sbjct: 1   MKDPRATYQTSAATPTSNMDALTTTLRNSIAALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGS 120
           DAH RDL+L+DGV+VPNVP+D+QCSSD RATENVPVCSFHKMAEYFN KS ISGNIPLGS
Sbjct: 61  DAHNRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSDISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQVDAAATKSLAM+GY+IPLFKV L +SNL L EDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLCEDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYV+EVGEQRF DSKS+ST  P
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVEEVGEQRFFDSKSRSTAAP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHA WA+TVKSAPDVINMTFTPIVSMLEGVPGVKHL R
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLVR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKP TGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMSKG 540
           GCTIRRSVWDHSPSTPATPLRSDGASSSS++RTSEDKKEDSS+NAGKLAKIVDLTEMSKG
Sbjct: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573

BLAST of Sgr019193 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 1.1e-253
Identity = 416/554 (75.09%), Postives = 481/554 (86.82%), Query Frame = 0

Query: 19  DALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLDDAHTRDLLLTDGVIVPNVP 78
           +ALTTTL N+IQALGRGFDVT+D+RLLYCKG PGS LV++++   RDL L+ G ++PNVP
Sbjct: 15  EALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVP 74

Query: 79  EDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGSFNAMFNFSGSWQVDAAATK 138
            DI CS  N  T+ + VCSFH+MAE FN++SG+ GNIPLG FNAMFN++GSWQVDAA+TK
Sbjct: 75  ADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTK 134

Query: 139 SLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGR 198
           SLA+VGYFIPL+ V+L    L LH +I+RAVP +WDP SLASFIEN+GTHI+TS TIGGR
Sbjct: 135 SLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGR 194

Query: 199 DVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFRRRGGDD 258
           DVVYIRQHQSSPL  SEIE+YV ++ + RF +++SQS  GPL YKDKD+TVIFRRRGGDD
Sbjct: 195 DVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGGDD 254

Query: 259 LVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFL 318
           L QSHA WAETV +APD+INMTFTPIVS+LEGVPG++HL RAI+LYLEYKPPIEDLQYFL
Sbjct: 255 LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFL 314

Query: 319 DFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPATGLRLNLEGCKQ 378
           D+QI RAWAPEQ+NLQRKEP+C SLQFSLMGPKL+IS DQ+TVGRKP TGLRL+LEG KQ
Sbjct: 315 DYQIARAWAPEQSNLQRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQ 374

Query: 379 NRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIE 438
           NRL+IHLQHLVSLPKILQPHWDSHV IGAPKW GPEEQDSRWFEPIKWKNFSHVST+PIE
Sbjct: 375 NRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIE 434

Query: 439 YTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATP 498
           +TE +IGDLSGVHIVTGAQLGVWDFG+KNVL+LKLLFSKVPGCTIRRSVWDH+P   +  
Sbjct: 435 HTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGR 494

Query: 499 LRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDK 558
           L   G S+SS +       E+ S  +GKLAKIVD +EM KGPQD+PGHWLVTGAKLGV+K
Sbjct: 495 LEPGGPSTSSST-------EEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEK 554

Query: 559 GRIVLRIKYSLLNY 573
           G+IVLR+KYSLLNY
Sbjct: 555 GKIVLRVKYSLLNY 561

BLAST of Sgr019193 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 6.4e-121
Identity = 249/590 (42.20%), Postives = 350/590 (59.32%), Query Frame = 0

Query: 28  SIQALGRGFDVTADIRLLYCK-GTPGSTLVQLDDAHTR-DLLLTDGVIVPNVPEDIQCSS 87
           +I ++G G+D+  D+RL YCK G+  S L+ + +     +++L  G+ +PNV + I+C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 88  DNRATENVPVCSFHKMAEYFNLKSGISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMVGY 147
             R      +  F +MAE FN +  ++G IP G FNAMF FS  WQ DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 148 FIPLFKVELINSNLALHEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQ 207
           FI L+ V L  S + L E +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 208 HQSSPLSESEIEHYVKEVGEQRFLDSK--------------------------SQSTVGP 267
             SS L   +++  +KEV ++RF+++                             S++G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 268 LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 327
            + K+  V +  RR G D+    H  W +TV+  PDVI+M+F PI S+L GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313

Query: 328 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL----QRKEPLCPSLQFSLMGPKLYIS 387
           AI+LYL YKPPIE+L  FL+FQ+ R WAP  + L    QRK+  C SLQFS  GPKLY++
Sbjct: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVN 373

Query: 388 PDQITVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEE 447
              + VG++P TG+RL LEG + NRLAIHLQHL SLPKI Q   D + +I        E 
Sbjct: 374 TTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI------RQES 433

Query: 448 QDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLF 507
            D R++E + WKN+SHV T P+E  +    DLS   +VTGAQL V   G KNVL+L+L F
Sbjct: 434 HDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFKNVLFLRLCF 493

Query: 508 SKVPGCT-IRRSVWDHSPS-TPATPLRSDGASSS-SKSRTSEDKKEDSSSN--------- 567
           S+V G T ++ S WD +    P + L S   S   + ++    +  D + N         
Sbjct: 494 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 553

Query: 568 ----AGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSL 570
               A KL K VD +EM++GPQ+ PG+W+V+GA+L V+KG+I L++KYSL
Sbjct: 554 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Sgr019193 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 2.2e-113
Identity = 235/599 (39.23%), Postives = 334/599 (55.76%), Query Frame = 0

Query: 28  SIQALGRGFDVTADIRLLYCKGTP-GSTLVQLDDAHTRDLLLTDGVIVPNVPEDIQCSSD 87
           ++  +G G+D+ +D+R   CK TP GS LV++D    RDL+   G++V NV   I+C   
Sbjct: 18  AVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCDKG 77

Query: 88  NRATENVPVCSFHKMAEYFNLKSGISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMVGYF 147
            R      + SF++M+E FN    +SG IP G FN MF FS  W  DA++ K+LA  G+F
Sbjct: 78  ERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDGWF 137

Query: 148 IPLFKVELINSNLALHEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQH 207
           I L+ VE++   L L +++KR VP +WD  +LA FIE +GTH++   T+GG+DV++++Q 
Sbjct: 138 ISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVKQM 197

Query: 208 QSSPLSESEIEHYVKEVGEQRF----LDSKSQSTVGP----------------------- 267
           + S     EI+  +K  G++RF    ++SKS ++V                         
Sbjct: 198 RKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVSSA 257

Query: 268 --LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHL 327
             +  K++++  +  RRGG DL QSH  W  TV  AP+VI+M F PI S+L G+PG   L
Sbjct: 258 VVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGTGFL 317

Query: 328 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPE----QTNLQRKEPLCPSLQFSLMGPKLY 387
           + A++LYL YKPPIE+L  FL+FQ+ R WAP        L+R +   PSLQFSLMGPKLY
Sbjct: 318 SHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPKLY 377

Query: 388 ISPDQITVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQ-PHWDSHVAIGAPKWHG 447
           ++  ++  G +P TGLR  LEG K N LAIHLQHL + P  L   H D++  I  P   G
Sbjct: 378 VNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVEKG 437

Query: 448 PEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLK 507
                  ++ P+KW  FSHV T P++Y  A   D +   IVT A L V   G + VL+L+
Sbjct: 438 -------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKVLFLR 497

Query: 508 LLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSKSR-----------TSEDKKEDSS 567
           L FS       R+S WD+     +T  R  G  S   +R           T    K D +
Sbjct: 498 LGFSLDASAVTRKSCWDNL----STNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDIN 557

Query: 568 SNA----------GKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLL 571
           S             KL  +VD  E+ +GP++ PG+W+VTGAKL V+ G+I ++ KYSLL
Sbjct: 558 SAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of Sgr019193 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.9e-109
Identity = 236/614 (38.44%), Postives = 332/614 (54.07%), Query Frame = 0

Query: 28  SIQALGRGFDVTADIRLLYCKGTPGST----LVQLDDAHTRDLLLTDGVIVPNVPEDIQC 87
           ++++LG+GFD+TAD RL YCK   GS     LV LD    R+L +    +  NV  DI C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 88  SSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGSFNAMFNF-SGSWQVDAAATKSLAM 147
               R      +  F+KM+EYFN +S ++G IP G+FNA F F SGSW  DAA  KSL +
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 148 VGYFIPLFKVELINSN-LALHEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVV 207
               + LF + + N N L L + ++ AVP +WDP  LA FIE +GTH+IT  ++GG+DVV
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 208 YIRQHQSSPLSESEIEHYVKEVGEQRF------------------------------LDS 267
            +RQ +SS L    + H++ ++G+Q F                               D 
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDD 251

Query: 268 KSQSTVGPLSYKDKD-VTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEG 327
           K        S   ++ +TVI  +RGGD   +SH+ W  TV   PD IN  F PI S+L+ 
Sbjct: 252 KQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKD 311

Query: 328 VPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL-----QRKEPLCPSLQF 387
           VPG   L+ A+ LYL YKPP+ DLQYFLDF   RAWAP   +L            P+L  
Sbjct: 312 VPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHI 371

Query: 388 SLMGPKLYISPDQITVGRKPATGLRLNLEGCKQNRLAIHLQHLVSL-PKILQPHWDSHVA 447
           + MGPKLY++   +T  + P TG+R  LEG K NRLAIHLQHL +    + +   D H+ 
Sbjct: 372 NFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI- 431

Query: 448 IGAPKWHGPEE--QDSRWFEPIKWKNFSHVSTAPIEY------TEANIGDLSGV-HIVTG 507
                W G ++   + R+FEP+  K FSHV T P++Y      T +N    + V  IVTG
Sbjct: 432 -----WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 491

Query: 508 AQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP----------STPATPLRSDGAS 567
           AQL V   G+K+VL+L+L ++KV    + ++ W H P          S+ + PL S    
Sbjct: 492 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 551

Query: 568 SSSKSRTSEDKKEDSSSNAG--------KLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVD 572
            +   +   +   DS    G        K+ K VDL+++ +GPQ  PGHWLVTG +L +D
Sbjct: 552 HNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLD 611

BLAST of Sgr019193 vs. ExPASy TrEMBL
Match: A0A6J1CMV2 (MACPF domain-containing protein CAD1 OS=Momordica charantia OX=3673 GN=LOC111012420 PE=4 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 546/575 (94.96%), Postives = 558/575 (97.04%), Query Frame = 0

Query: 1   MKDPCATQTAA---VTATSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQ 60
           MKDP A+QT A    T+TS+MDALT TL NSIQALGRGFDVTADIRLLYCKGTPGS L+Q
Sbjct: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60

Query: 61  LDDAHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPL 120
           LDDAHTRDL+LTDGV+VPNVP+DIQCSSDNRATENVPVC+FHKMAEYFN KSGISGNIPL
Sbjct: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFNAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQVDAAATKSLAMVGY IPLFKVEL NSNL L EDIKRAVPYTWDPVS
Sbjct: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHL 300
           GPLSYKDKDVTVIFRRRGGDDLVQSHA WA+TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKP TGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSTPATPLRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPS PATPLRSDGASSSSK+RTSEDKKEDSSSNAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575

BLAST of Sgr019193 vs. ExPASy TrEMBL
Match: A0A5D3CPE6 (MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008470 PE=4 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 539/573 (94.07%), Postives = 553/573 (96.51%), Query Frame = 0

Query: 1   MKDPCAT-QTAAVTATSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLD 60
           MKDP AT QTAA TATS MDALTTTL NSI ALGRGFDVTADIRLLYCKGTPGS LVQLD
Sbjct: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGS 120
           DAHTRDL+L+DGV+VPNVP+D+QCSSD RATENVPVCSFHKMAEYFN KSGISGNIPLGS
Sbjct: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQ+DAAATKSLAM+GY+IP FKV L +SNL L EDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQS   P
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHA WA+TVKSAPDVINMTFTPIVSMLEGVPGVKHLAR
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKP TGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMSKG 540
           GCTIRRSVWDHSPSTPATPLRSDGASSSS++RTSEDKKEDSS+NAGKLAKIVDLTEMSKG
Sbjct: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           PQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 573

BLAST of Sgr019193 vs. ExPASy TrEMBL
Match: A0A1S3AZC7 (MACPF domain-containing protein CAD1 OS=Cucumis melo OX=3656 GN=LOC103484421 PE=4 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 539/573 (94.07%), Postives = 553/573 (96.51%), Query Frame = 0

Query: 1   MKDPCAT-QTAAVTATSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLD 60
           MKDP AT QTAA TATS MDALTTTL NSI ALGRGFDVTADIRLLYCKGTPGS LVQLD
Sbjct: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGS 120
           DAHTRDL+L+DGV+VPNVP+D+QCSSD RATENVPVCSFHKMAEYFN KSGISGNIPLGS
Sbjct: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQ+DAAATKSLAM+GY+IP FKV L +SNL L EDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQS   P
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHA WA+TVKSAPDVINMTFTPIVSMLEGVPGVKHLAR
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKP TGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMSKG 540
           GCTIRRSVWDHSPSTPATPLRSDGASSSS++RTSEDKKEDSS+NAGKLAKIVDLTEMSKG
Sbjct: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           PQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 573

BLAST of Sgr019193 vs. ExPASy TrEMBL
Match: A0A6J1EGA5 (MACPF domain-containing protein CAD1 OS=Cucurbita moschata OX=3662 GN=LOC111433834 PE=4 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 535/573 (93.37%), Postives = 546/573 (95.29%), Query Frame = 0

Query: 1   MKDPCATQTAAVTA-TSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLD 60
           MKDPC T  AA T  TS MDALT TL NSIQALGRGFDVTADIRLLYCKGTPGS LVQLD
Sbjct: 1   MKDPCTTYAAAATTPTSEMDALTATLCNSIQALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGS 120
           DA TRDL L+DGV+VPNVP+ + CSSD RATENVPVCSFHKMAEYFN KS +SGNIPLGS
Sbjct: 61  DASTRDLHLSDGVVVPNVPDHVHCSSDTRATENVPVCSFHKMAEYFNQKSNLSGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQVDAAATKSLAM+GYFIPLFKVEL NS L LHE+IKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQVDAAATKSLAMIGYFIPLFKVELKNSTLVLHEEIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIE+YVKEVGEQRFLDSKSQSTVGP
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIENYVKEVGEQRFLDSKSQSTVGP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHA WAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKP TGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGA KWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGALKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMSKG 540
           GCT+RRSVWDHSPST ATP RSDGASSSSK++T EDKKEDSSSNAGKLAKIVDLTEMSKG
Sbjct: 481 GCTVRRSVWDHSPSTSATPSRSDGASSSSKTKTGEDKKEDSSSNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573

BLAST of Sgr019193 vs. ExPASy TrEMBL
Match: A0A5A7U9L9 (MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G002000 PE=4 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 539/600 (89.83%), Postives = 553/600 (92.17%), Query Frame = 0

Query: 1   MKDPCAT-QTAAVTATSAMDALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLD 60
           MKDP AT QTAA TATS MDALTTTL NSI ALGRGFDVTADIRLLYCKGTPGS LVQLD
Sbjct: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLLLTDGVIVPNVPEDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGS 120
           DAHTRDL+L+DGV+VPNVP+D+QCSSD RATENVPVCSFHKMAEYFN KSGISGNIPLGS
Sbjct: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQ+DAAATKSLAM+GY+IP FKV L +SNL L EDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQS   P
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAP 240

Query: 241 LSYKDK---------------------------DVTVIFRRRGGDDLVQSHANWAETVKS 300
           LSYKDK                           DVTVIFRRRGGDDLVQSHA WA+TVKS
Sbjct: 241 LSYKDKVSRQQASSLFSWTVFSEHFTHDPSFLQDVTVIFRRRGGDDLVQSHAQWAKTVKS 300

Query: 301 APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTN 360
           APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTN
Sbjct: 301 APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTN 360

Query: 361 LQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLP 420
           LQRKEPLCPSLQFSLMGPKLYISPDQITVGRKP TGLRLNLEGCKQNRLAIHLQHLVSLP
Sbjct: 361 LQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLP 420

Query: 421 KILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHI 480
           KILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHI
Sbjct: 421 KILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHI 480

Query: 481 VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSKSRT 540
           VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSS++RT
Sbjct: 481 VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSRTRT 540

Query: 541 SEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573
           SEDKKEDSS+NAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 SEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 600

BLAST of Sgr019193 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 877.1 bits (2265), Expect = 7.7e-255
Identity = 416/554 (75.09%), Postives = 481/554 (86.82%), Query Frame = 0

Query: 19  DALTTTLFNSIQALGRGFDVTADIRLLYCKGTPGSTLVQLDDAHTRDLLLTDGVIVPNVP 78
           +ALTTTL N+IQALGRGFDVT+D+RLLYCKG PGS LV++++   RDL L+ G ++PNVP
Sbjct: 15  EALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVP 74

Query: 79  EDIQCSSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGSFNAMFNFSGSWQVDAAATK 138
            DI CS  N  T+ + VCSFH+MAE FN++SG+ GNIPLG FNAMFN++GSWQVDAA+TK
Sbjct: 75  ADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTK 134

Query: 139 SLAMVGYFIPLFKVELINSNLALHEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGR 198
           SLA+VGYFIPL+ V+L    L LH +I+RAVP +WDP SLASFIEN+GTHI+TS TIGGR
Sbjct: 135 SLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGR 194

Query: 199 DVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFRRRGGDD 258
           DVVYIRQHQSSPL  SEIE+YV ++ + RF +++SQS  GPL YKDKD+TVIFRRRGGDD
Sbjct: 195 DVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGGDD 254

Query: 259 LVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFL 318
           L QSHA WAETV +APD+INMTFTPIVS+LEGVPG++HL RAI+LYLEYKPPIEDLQYFL
Sbjct: 255 LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFL 314

Query: 319 DFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPATGLRLNLEGCKQ 378
           D+QI RAWAPEQ+NLQRKEP+C SLQFSLMGPKL+IS DQ+TVGRKP TGLRL+LEG KQ
Sbjct: 315 DYQIARAWAPEQSNLQRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQ 374

Query: 379 NRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIE 438
           NRL+IHLQHLVSLPKILQPHWDSHV IGAPKW GPEEQDSRWFEPIKWKNFSHVST+PIE
Sbjct: 375 NRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIE 434

Query: 439 YTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATP 498
           +TE +IGDLSGVHIVTGAQLGVWDFG+KNVL+LKLLFSKVPGCTIRRSVWDH+P   +  
Sbjct: 435 HTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGR 494

Query: 499 LRSDGASSSSKSRTSEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDK 558
           L   G S+SS +       E+ S  +GKLAKIVD +EM KGPQD+PGHWLVTGAKLGV+K
Sbjct: 495 LEPGGPSTSSST-------EEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEK 554

Query: 559 GRIVLRIKYSLLNY 573
           G+IVLR+KYSLLNY
Sbjct: 555 GKIVLRVKYSLLNY 561

BLAST of Sgr019193 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 436.0 bits (1120), Expect = 4.6e-122
Identity = 249/590 (42.20%), Postives = 350/590 (59.32%), Query Frame = 0

Query: 28  SIQALGRGFDVTADIRLLYCK-GTPGSTLVQLDDAHTR-DLLLTDGVIVPNVPEDIQCSS 87
           +I ++G G+D+  D+RL YCK G+  S L+ + +     +++L  G+ +PNV + I+C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 88  DNRATENVPVCSFHKMAEYFNLKSGISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMVGY 147
             R      +  F +MAE FN +  ++G IP G FNAMF FS  WQ DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 148 FIPLFKVELINSNLALHEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQ 207
           FI L+ V L  S + L E +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 208 HQSSPLSESEIEHYVKEVGEQRFLDSK--------------------------SQSTVGP 267
             SS L   +++  +KEV ++RF+++                             S++G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 268 LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 327
            + K+  V +  RR G D+    H  W +TV+  PDVI+M+F PI S+L GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313

Query: 328 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL----QRKEPLCPSLQFSLMGPKLYIS 387
           AI+LYL YKPPIE+L  FL+FQ+ R WAP  + L    QRK+  C SLQFS  GPKLY++
Sbjct: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVN 373

Query: 388 PDQITVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEE 447
              + VG++P TG+RL LEG + NRLAIHLQHL SLPKI Q   D + +I        E 
Sbjct: 374 TTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI------RQES 433

Query: 448 QDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLF 507
            D R++E + WKN+SHV T P+E  +    DLS   +VTGAQL V   G KNVL+L+L F
Sbjct: 434 HDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFKNVLFLRLCF 493

Query: 508 SKVPGCT-IRRSVWDHSPS-TPATPLRSDGASSS-SKSRTSEDKKEDSSSN--------- 567
           S+V G T ++ S WD +    P + L S   S   + ++    +  D + N         
Sbjct: 494 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 553

Query: 568 ----AGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSL 570
               A KL K VD +EM++GPQ+ PG+W+V+GA+L V+KG+I L++KYSL
Sbjct: 554 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Sgr019193 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 411.0 bits (1055), Expect = 1.6e-114
Identity = 235/599 (39.23%), Postives = 334/599 (55.76%), Query Frame = 0

Query: 28  SIQALGRGFDVTADIRLLYCKGTP-GSTLVQLDDAHTRDLLLTDGVIVPNVPEDIQCSSD 87
           ++  +G G+D+ +D+R   CK TP GS LV++D    RDL+   G++V NV   I+C   
Sbjct: 18  AVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCDKG 77

Query: 88  NRATENVPVCSFHKMAEYFNLKSGISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMVGYF 147
            R      + SF++M+E FN    +SG IP G FN MF FS  W  DA++ K+LA  G+F
Sbjct: 78  ERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDGWF 137

Query: 148 IPLFKVELINSNLALHEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQH 207
           I L+ VE++   L L +++KR VP +WD  +LA FIE +GTH++   T+GG+DV++++Q 
Sbjct: 138 ISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVKQM 197

Query: 208 QSSPLSESEIEHYVKEVGEQRF----LDSKSQSTVGP----------------------- 267
           + S     EI+  +K  G++RF    ++SKS ++V                         
Sbjct: 198 RKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVSSA 257

Query: 268 --LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHL 327
             +  K++++  +  RRGG DL QSH  W  TV  AP+VI+M F PI S+L G+PG   L
Sbjct: 258 VVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGTGFL 317

Query: 328 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPE----QTNLQRKEPLCPSLQFSLMGPKLY 387
           + A++LYL YKPPIE+L  FL+FQ+ R WAP        L+R +   PSLQFSLMGPKLY
Sbjct: 318 SHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPKLY 377

Query: 388 ISPDQITVGRKPATGLRLNLEGCKQNRLAIHLQHLVSLPKILQ-PHWDSHVAIGAPKWHG 447
           ++  ++  G +P TGLR  LEG K N LAIHLQHL + P  L   H D++  I  P   G
Sbjct: 378 VNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVEKG 437

Query: 448 PEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLK 507
                  ++ P+KW  FSHV T P++Y  A   D +   IVT A L V   G + VL+L+
Sbjct: 438 -------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKVLFLR 497

Query: 508 LLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSKSR-----------TSEDKKEDSS 567
           L FS       R+S WD+     +T  R  G  S   +R           T    K D +
Sbjct: 498 LGFSLDASAVTRKSCWDNL----STNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDIN 557

Query: 568 SNA----------GKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLL 571
           S             KL  +VD  E+ +GP++ PG+W+VTGAKL V+ G+I ++ KYSLL
Sbjct: 558 SAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of Sgr019193 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 397.9 bits (1021), Expect = 1.4e-110
Identity = 236/614 (38.44%), Postives = 332/614 (54.07%), Query Frame = 0

Query: 28  SIQALGRGFDVTADIRLLYCKGTPGST----LVQLDDAHTRDLLLTDGVIVPNVPEDIQC 87
           ++++LG+GFD+TAD RL YCK   GS     LV LD    R+L +    +  NV  DI C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 88  SSDNRATENVPVCSFHKMAEYFNLKSGISGNIPLGSFNAMFNF-SGSWQVDAAATKSLAM 147
               R      +  F+KM+EYFN +S ++G IP G+FNA F F SGSW  DAA  KSL +
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 148 VGYFIPLFKVELINSN-LALHEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVV 207
               + LF + + N N L L + ++ AVP +WDP  LA FIE +GTH+IT  ++GG+DVV
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 208 YIRQHQSSPLSESEIEHYVKEVGEQRF------------------------------LDS 267
            +RQ +SS L    + H++ ++G+Q F                               D 
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDD 251

Query: 268 KSQSTVGPLSYKDKD-VTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEG 327
           K        S   ++ +TVI  +RGGD   +SH+ W  TV   PD IN  F PI S+L+ 
Sbjct: 252 KQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKD 311

Query: 328 VPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL-----QRKEPLCPSLQF 387
           VPG   L+ A+ LYL YKPP+ DLQYFLDF   RAWAP   +L            P+L  
Sbjct: 312 VPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHI 371

Query: 388 SLMGPKLYISPDQITVGRKPATGLRLNLEGCKQNRLAIHLQHLVSL-PKILQPHWDSHVA 447
           + MGPKLY++   +T  + P TG+R  LEG K NRLAIHLQHL +    + +   D H+ 
Sbjct: 372 NFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI- 431

Query: 448 IGAPKWHGPEE--QDSRWFEPIKWKNFSHVSTAPIEY------TEANIGDLSGV-HIVTG 507
                W G ++   + R+FEP+  K FSHV T P++Y      T +N    + V  IVTG
Sbjct: 432 -----WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 491

Query: 508 AQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP----------STPATPLRSDGAS 567
           AQL V   G+K+VL+L+L ++KV    + ++ W H P          S+ + PL S    
Sbjct: 492 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 551

Query: 568 SSSKSRTSEDKKEDSSSNAG--------KLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVD 572
            +   +   +   DS    G        K+ K VDL+++ +GPQ  PGHWLVTG +L +D
Sbjct: 552 HNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLD 611

BLAST of Sgr019193 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 229.6 bits (584), Expect = 6.5e-60
Identity = 122/310 (39.35%), Postives = 178/310 (57.42%), Query Frame = 0

Query: 28  SIQALGRGFDVTADIRLLYCK-GTPGSTLVQLDDAHTR-DLLLTDGVIVPNVPEDIQCSS 87
           +I ++G G+D+  D+RL YCK G+  S L+ + +     +++L  G+ +PNV + I+C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 88  DNRATENVPVCSFHKMAEYFNLKSGISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMVGY 147
             R      +  F +MAE FN +  ++G IP G FNAMF FS  WQ DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 148 FIPLFKVELINSNLALHEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQ 207
           FI L+ V L  S + L E +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 208 HQSSPLSESEIEHYVKEVGEQRFLDSK--------------------------SQSTVGP 267
             SS L   +++  +KEV ++RF+++                             S++G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 268 LSYKDKDVTVIFRRRGGDDLVQSHANWAETVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 310
            + K+  V +  RR G D+    H  W +TV+  PDVI+M+F PI S+L GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313