Sgr019189 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr019189 vs. NCBI nr
Match: XP_038882517.1 (subtilisin-like protease SBT5.6 [Benincasa hispida])

HSP 1 Score: 1328.9 bits (3438), Expect = 0.0e+00
Identity = 659/783 (84.16%), Postives = 714/783 (91.19%), Query Frame = 0

Query: 1   MEKSTFVCLLLLLLL-LLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVK 60
           MEKS+F C +LLLLL  + LQAS S   K AYIVYFGEH GDK+W+EIEA HHSYL+SVK
Sbjct: 1   MEKSSFFCFVLLLLLPFIQLQASSSHNHK-AYIVYFGEHHGDKSWEEIEARHHSYLMSVK 60

Query: 61  DTEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSG 120
           ++EE+AK+SLLY+YKHSIN FAA+LT  +ASKLSELDEVVSVI S+KY  HTTRSWEFSG
Sbjct: 61  ESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSG 120

Query: 121 VEEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFE 180
           VEEDKP INDLV RA YGKDVVIGMLDSGVWPKS+SFSDKGMGPIPKSWKGIC TGP F+
Sbjct: 121 VEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQ 180

Query: 181 PSHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFG 240
            +HCNRKIIGARYYLKGYE+ FGRLNET DYRSPCDKDGHGSHTASIAGG+RVYNVSAFG
Sbjct: 181 SAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFG 240

Query: 241 GVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSI 300
           GVAWGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD LAAMDDAIADGVDVLSLSI
Sbjct: 241 GVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSI 300

Query: 301 GKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREF 360
           GK DPYNYTDDGIAIGALHAVK+DIVVSCSAGNYGPTPSALSNIAPWIITVGAST+DREF
Sbjct: 301 GKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREF 360

Query: 361 YSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKAR 420
           YSPVILGNGLKIKG SVAPSKLKRKKMYPLVYAGDI+KPHVPRN++GLCVA SLSHEKA+
Sbjct: 361 YSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAK 420

Query: 421 GKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANI 480
           GKIVLC+RG GISR+AGSLEVKRSGGAGMILGNVPAVGR+ H+DPHFVPATAVSYEDAN+
Sbjct: 421 GKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANM 480

Query: 481 ILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAA 540
           ILKYIKSR NPTA I+PPITIYGSRPAPAMANFSSRGPN +DPH LKPDITAPGVDILAA
Sbjct: 481 ILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAA 540

Query: 541 WSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTT 600
           WS +DSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTT
Sbjct: 541 WSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTT 600

Query: 601 ATARNKAGMPITEDAT-DHS-ATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGM 660
           AT  NK+G P+T+DA+ D+S A+PFS+GSGHF PSKAADPGLVYD++YTDYLHYLCGL M
Sbjct: 601 ATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKM 660

Query: 661 NSIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV-GGSRSVYLFYSRPPPGV 720
           NSIDP+F CP RAL   DLNYPSIAVP+LRGAVRI+RTVTNV GG +SVY F+S  PPGV
Sbjct: 661 NSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPGV 720

Query: 721 IVRASPSILYFRGVGEKKSFTITVSGKAK---IGVDSSGYSFGWYAWSDGIHHVRSPIAV 777
            V ASP+ILYF  VGE+K FTITVSGK K      +++GYSFGW+AW+DGIH+VRSPIAV
Sbjct: 721 AVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIAV 780

BLAST of Sgr019189 vs. NCBI nr
Match: XP_022142085.1 (subtilisin-like protease SBT5.6 [Momordica charantia])

HSP 1 Score: 1326.2 bits (3431), Expect = 0.0e+00
Identity = 665/785 (84.71%), Postives = 713/785 (90.83%), Query Frame = 0

Query: 1   MEK-STFVCLLLLLLLLLPLQASCSDKQKQ-AYIVYFGEHRGDKAWQEIEANHHSYLLSV 60
           MEK STFVCLLLLLLLLL  QAS S    Q AYIVYFGEHRGDKAW EIEA HHSYL SV
Sbjct: 32  MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 91

Query: 61  KDTEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFS 120
           KDTEEEA++SL+YSYKHSINGFAA+L+++EASKLSE+D VV VIRS+KYST TTRSWEFS
Sbjct: 92  KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVRVIRSEKYSTQTTRSWEFS 151

Query: 121 GVEEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGF 180
           GVEEDKPR+NDLVSRAK+GKDVVIGMLDSGVWPKS+SFSDKGMGPIPKSW+GIC TGP F
Sbjct: 152 GVEEDKPRLNDLVSRAKFGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWRGICQTGPAF 211

Query: 181 EPSHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAF 240
           + +HCNRKIIGARYYLKGYEN FGRLNETEDYRSPCDKDGHGSHTAS AGG+RV+NVSAF
Sbjct: 212 QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF 271

Query: 241 GGVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLS 300
           GGVA GTASGGAP  RLAIYKVCWAIP QMKILGNVCFDTD LAAMDDAIADGVDVLSLS
Sbjct: 272 GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS 331

Query: 301 IGKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDRE 360
           IGK DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSN APWIITVGAST+DRE
Sbjct: 332 IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE 391

Query: 361 FYSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKA 420
           FYSPVILGNG KI+G SVAPS LKRKKMYPLVYAGDIMKPHVP+N++G+CVA SLSHEKA
Sbjct: 392 FYSPVILGNGFKIRGLSVAPSMLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA 451

Query: 421 RGKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDAN 480
           RGKIVLCYR  GISRYAGSLEVKRSGGAGMILGNVPAVGRKLH+DPHFVPATAVSYEDAN
Sbjct: 452 RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN 511

Query: 481 IILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILA 540
           IILKYIKSR NPTATI+PPITIYGSRPAPAMANFSSRGPN IDPHILKPDITAPGVDILA
Sbjct: 512 IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA 571

Query: 541 AWSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMT 600
           AWSGEDSPTKLPK LDPR+V YNLYSGTSMSCPHV+AAAALLRAIHP+WSQAAIRSALMT
Sbjct: 572 AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT 631

Query: 601 TATARNKAGMPITEDATDHS--ATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLG 660
           TAT  NK G PIT+DA+DHS  ATPFSYGSGHF PSKAADPGLVYDA+YTDYL +LC L 
Sbjct: 632 TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK 691

Query: 661 M-NSIDPTFKCPARALPAQDLNYPSIAVPKLR----GAVRIRRTVTNVGGSRSVYLFYSR 720
           M +SIDP+F CPARA  A DLNYPS+AVP+LR    G VR+ RTV NVGGS+S Y F + 
Sbjct: 692 MAHSIDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGGVRVVRTVRNVGGSKSAYFFRAS 751

Query: 721 PPPGVIVRASPSILYFRGVGEKKSFTITVSGKAKI-GVDSSGYSFGWYAWSDGIHHVRSP 776
            P GV VRASPS+LYF  +G++K FTIT+SGKA+I GVD SGYSFGW+AW+DGIH VRSP
Sbjct: 752 APAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSP 811

BLAST of Sgr019189 vs. NCBI nr
Match: XP_022926980.1 (subtilisin-like protease SBT5.6 [Cucurbita moschata])

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 643/779 (82.54%), Postives = 702/779 (90.12%), Query Frame = 0

Query: 1   MEKSTFVCLLLLLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKD 60
           ME S+  CLLLLLLLL P  +SC   QKQAYIVYFGEH GDK+W+EIE  HHSYL+SVKD
Sbjct: 1   MEISSLFCLLLLLLLLPP--SSCL-AQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKD 60

Query: 61  TEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGV 120
           TE +AK+SLLYSYKH+INGFAA+LT  +ASKLSEL+EVVSVI S+KY  HTTRSWEFSGV
Sbjct: 61  TENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTTRSWEFSGV 120

Query: 121 EEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFEP 180
           EEDKPR++DLVS+AK GKDVVIGMLDSGVWPKS+SFSDKGMGP+PKSWKGIC TGP FE 
Sbjct: 121 EEDKPRLSDLVSKAKAGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPAFES 180

Query: 181 SHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFGG 240
           SHCNRKIIGARYY+KGYE+ FGRLNET D+RSPCD DGHGSHTASIAGG+RVYNVSAFGG
Sbjct: 181 SHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGG 240

Query: 241 VAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSIG 300
           VA GTASGGAP VRLAIYKVCWAIPNQMK LGNVCFDTD LAAMDDAI+DGVDVLSLSIG
Sbjct: 241 VARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIG 300

Query: 301 KEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREFY 360
           K DPYNYTDDGIAIGALHAVK+DIVVSCSAGN GPTPS LSN+APWIITVGAST+DREFY
Sbjct: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFY 360

Query: 361 SPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKARG 420
           SPVILGNGLKIKG SVAPSKL+R KMYPLVYA DI KPHVPRNE+G C+A SLSH+KA+G
Sbjct: 361 SPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKG 420

Query: 421 KIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANII 480
           KIVLCYRG GISRYAGSLEVKRSGGAGMILG VPAVG +LH+DPHFVPATAVSY+DAN I
Sbjct: 421 KIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRI 480

Query: 481 LKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAAW 540
           LKYIKS RNPTATI+PPITIYGSRPAPAMANF+SRGP+L+DPH LKPDITAPGVDILAAW
Sbjct: 481 LKYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAW 540

Query: 541 SGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTA 600
           S +DSPTKLPK  DPRIVKYN+YSGTSMSCPHVSAAAALLRAIHP+WSQAAIRSALMTTA
Sbjct: 541 SEKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTA 600

Query: 601 TARNKAGMPITEDA-TDHS-ATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMN 660
           T  NK G PIT+D+ TD+S ATPFS+GSGHF P+KAADPGLVYDA+YTDYLHYLCGL MN
Sbjct: 601 TTTNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV-GGSRSVYLFYSRPPPGVI 720
           SID +FKCP RAL   DLNYPSIA+PKL+G VRI+RTVTNV GG +SVY F S  PPGV 
Sbjct: 661 SIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGVA 720

Query: 721 VRASPSILYFRGVGEKKSFTITVSGKAKIGVDSSGYSFGWYAWSDGIHHVRSPIAVSST 777
           VRASPS+LYF  VG++K FTITVSGK K     +GYSFGW+AWSDG+H+VRSPIA+SST
Sbjct: 721 VRASPSVLYFDRVGQRKRFTITVSGKVK----GNGYSFGWFAWSDGVHYVRSPIAISST 772

BLAST of Sgr019189 vs. NCBI nr
Match: XP_023517293.1 (subtilisin-like protease SBT5.6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 640/779 (82.16%), Postives = 702/779 (90.12%), Query Frame = 0

Query: 1   MEKSTFVCLLLLLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKD 60
           ME S+  CLLLLLLLLL   +SC   QKQAYIVYFGEH GDK+W+EIE  HHSYL+SVKD
Sbjct: 1   METSSLFCLLLLLLLLLLPPSSCL-AQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKD 60

Query: 61  TEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGV 120
           TE +AK+SLLYSYKH+INGFAA+LT  +ASKLSEL+EVVSVI S++Y  HTTRSWEFSGV
Sbjct: 61  TENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKRYGMHTTRSWEFSGV 120

Query: 121 EEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFEP 180
           EEDKPR++DLVS+AK GKDVVIGMLDSGVWPKS+SFSDKGMGP+PKSWKGIC TGPGF+ 
Sbjct: 121 EEDKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPGFQS 180

Query: 181 SHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFGG 240
            HCNRKIIGARYY+KGYE+ FGRLNET D+RSPCD DGHGSHTASIAGG+RVYNVSAFGG
Sbjct: 181 PHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGG 240

Query: 241 VAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSIG 300
           VA GTASGGAP VRLAIYKVCWAIPNQMK LGNVCFDTD LAAMDDAI+DGVDVLSLSIG
Sbjct: 241 VARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIG 300

Query: 301 KEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREFY 360
           K DPYNYTDDGIAIGALHAVK+DIVVSCSAGN GPTPS LSN+APWIITVGAST+DREFY
Sbjct: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFY 360

Query: 361 SPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKARG 420
           SPVILGNGLKIKG SVAPSKL+R KMYPLVYA DI KPHVPRNE+G C+A SLSH+KA+G
Sbjct: 361 SPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKG 420

Query: 421 KIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANII 480
           KIVLCYRG GISRYAGSLEVKRSGGAGMILG VPAVG +LH+DPHFVPATAVSY+DAN I
Sbjct: 421 KIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRI 480

Query: 481 LKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAAW 540
           LKYIKS RNPTATI+PPITIYGSRPAPAMA F+SRGP+L+DPH LKPDITAPGVDILAAW
Sbjct: 481 LKYIKSHRNPTATIVPPITIYGSRPAPAMAYFTSRGPSLVDPHFLKPDITAPGVDILAAW 540

Query: 541 SGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTA 600
           S +DSPTKLPK  DPRIVKYN+YSGTSMSCPHVSAAAALLRAIHP+WSQAAIRSALMTTA
Sbjct: 541 SEKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTA 600

Query: 601 TARNKAGMPITEDA-TDHS-ATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMN 660
           T  NK G PIT+D+ TD+S ATPFS+GSGHF P+KAADPGLVYDA+YTDYLHYLCGL MN
Sbjct: 601 TTTNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV-GGSRSVYLFYSRPPPGVI 720
           SIDP+FKCP RAL   DLNYPSIA+PKL+G VRI+RTVTNV GG ++VY F S  PPGV 
Sbjct: 661 SIDPSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKTVYFFESWAPPGVA 720

Query: 721 VRASPSILYFRGVGEKKSFTITVSGKAKIGVDSSGYSFGWYAWSDGIHHVRSPIAVSST 777
           VRASPS+LYF  VG++K FT+TVSGK K     +GYSFGW+AWSDGIH+VRSPIA+SST
Sbjct: 721 VRASPSVLYFDRVGQRKRFTVTVSGKVK----GNGYSFGWFAWSDGIHYVRSPIAISST 774

BLAST of Sgr019189 vs. NCBI nr
Match: XP_004134922.1 (subtilisin-like protease SBT5.6 isoform X2 [Cucumis sativus] >KGN49345.1 hypothetical protein Csa_003109 [Cucumis sativus])

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 646/791 (81.67%), Postives = 707/791 (89.38%), Query Frame = 0

Query: 1   MEKSTFVC----LLLLLLLLLPL----QASCSDKQKQAYIVYFGEHRGDKAWQEIEANHH 60
           MEKS+F+C    LLLLLLLL+ L     AS S+ QK+AYIVYFGEH G+K+ +EI+  HH
Sbjct: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60

Query: 61  SYLLSVKDTEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTT 120
           SYL+ VK++EE+AK+ LLY+YKHSIN FAA+LT  +ASKLS+LDEVVSVI S+KY   TT
Sbjct: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120

Query: 121 RSWEFSGVEEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKP INDLVSRA YGKDVVIGMLDSGVWPKS+SFSDKGMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 HTGPGFEPSHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRV 240
            TGP F+ +HCNRKIIGARYYLKGYE+ FGRLN+T DYRSPCDKDGHGSHTASIAGG+RV
Sbjct: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGV 300
           YNVSAFGGVAWGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD LAAMDDAIADGV
Sbjct: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300

Query: 301 DVLSLSIGKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGA 360
           DVLSLSIGK +PYNYTDDG+AIGALHAVKKDIVVSCSAGNYGPTPSALSN+APWIITVGA
Sbjct: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360

Query: 361 STLDREFYSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAES 420
           ST+DREFYSPVILGNGLKIKG SVAPSKL+RKKMYPLVYAGDIM PH PRN++GLCVA S
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420

Query: 421 LSHEKARGKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAV 480
           LSHEKA+GKIVLC+RG GISR+AGSLEV+RSGGAGMILGNVPAVGR+ H+DPHFVPATAV
Sbjct: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480

Query: 481 SYEDANIILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAP 540
           SYEDANIILKYIKSR+NPTATI+PP+TIYGSRPAPAMANFSSRGPN IDPH LKPDITAP
Sbjct: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540

Query: 541 GVDILAAWSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAI 600
           GVDILAAWS +DSPTKLPKYLDPRIV+YNLYSGTSMSCPHVSAAAALLRAIHP+WSQAAI
Sbjct: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600

Query: 601 RSALMTTATARNKAGMPITEDAT-DHS-ATPFSYGSGHFCPSKAADPGLVYDADYTDYLH 660
           RSALMTT+T  NK G PIT+D+T D+S ATPFS+GSGHF PSKAADPGLVYD++YTDYLH
Sbjct: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660

Query: 661 YLCGLGMNSIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV-GGSRSVYLFY 720
           YLCGL MNSIDP+FKCP RAL   DLNYPSIAVP+LR  VRI+RTVTNV GG ++VY F 
Sbjct: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720

Query: 721 SRPPPGVIVRASPSILYFRGVGEKKSFTITVSGKAKIGVDSS----GYSFGWYAWSDGIH 777
           S  P GV V ASP+ILYF  VGE+K FTIT+S K      SS     YSFGW+AWSDGIH
Sbjct: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780

BLAST of Sgr019189 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 3.8e-257
Identity = 460/790 (58.23%), Postives = 569/790 (72.03%), Query Frame = 0

Query: 10  LLLLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKDTEEEAKASL 69
           L  LL L+PL ASC++ +KQ YIVYFGEH+GDKA+ EIE +HHSYL SVK++EE+A+ASL
Sbjct: 7   LFPLLFLIPLLASCAE-EKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASL 66

Query: 70  LYSYKHSINGFAALLTADEASKLSELDEVVSVIRS--QKYSTHTTRSWEFSGVEEDKP-- 129
           LYSYKHSINGFAA LT D+ASKL +L EVVSV +S  +KY  HTTRSWEF G+EE++   
Sbjct: 67  LYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDS 126

Query: 130 ----RIND----------LVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGI 189
               R ND           + +AK+G  +++G+LDSGVWP+S+SF+DKGMGP+PKSWKGI
Sbjct: 127 DVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGI 186

Query: 190 CHTGPGFEPSHCNRKIIGARYYLKGYENQFGRLNET--EDYRSPCDKDGHGSHTASIAGG 249
           C TG  F  SHCNRKIIGARYY+KGYE  +G  N T  +D+ SP D DGHGSHTAS A G
Sbjct: 187 CQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVG 246

Query: 250 QRVYNVSAFGGVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIA 309
           +RV   SA GG A G+ASGGAP  RLAIYK CWA PN  K+ GN+C + D LAA+DDAIA
Sbjct: 247 RRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIA 306

Query: 310 DGVDVLSLSIGKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIIT 369
           DGV V+S+SIG  +P+ +T DGIA+GALHAVK++IVV+ SAGN GP P  LSN+APWIIT
Sbjct: 307 DGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIIT 366

Query: 370 VGASTLDREFYSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCV 429
           VGASTLDR F   ++LGNG  IK  S+  +  K  K  PLVYA +++ P +  NET  C+
Sbjct: 367 VGASTLDRAFVGGLVLGNGYTIKTDSI--TAFKMDKFAPLVYASNVVVPGIALNETSQCL 426

Query: 430 AESLSHEKARGKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPA 489
             SL  E   GK+VLC RG G SR    +EVKR+GGAGMILGN+ A G ++ SD HFVP 
Sbjct: 427 PNSLKPELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPT 486

Query: 490 TAVSYEDANIILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDI 549
             V+    + IL+YIK+ +NP A I P  T+Y  + AP+M  FSSRGPN++DP+ILKPDI
Sbjct: 487 AGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDI 546

Query: 550 TAPGVDILAAWSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQ 609
           TAPG+ ILAAWSG DSP+K+   +D R+  YN+YSGTSMSCPHV+ A ALL+AIHP WS 
Sbjct: 547 TAPGLYILAAWSGADSPSKMS--VDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSS 606

Query: 610 AAIRSALMTTATARNKAGMPITEDATDHSATPFSYGSGHFCPSKAADPGLVYDADYTDYL 669
           AAIRSALMTTA   N    PI +D T   A PF+ GSGHF P+KAADPGLVYDA Y  YL
Sbjct: 607 AAIRSALMTTAWMTNDKKKPI-QDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYL 666

Query: 670 HYLCGLGMNSIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV--GGSRSVYL 729
            Y C + + +IDPTFKCP++  P  + NYPSIAVP L+  V ++RTVTNV  G S S YL
Sbjct: 667 LYGCSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYL 726

Query: 730 FYSRPPPGVIVRASPSILYFRGVGEKKSFTITV---SGKAKIGVDSSGYSFGWYAWSDGI 775
           F  +PP G+ V+A P+IL F  +G+K+ F I +     +     +   Y FGW++W+D +
Sbjct: 727 FSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKV 786

BLAST of Sgr019189 vs. ExPASy Swiss-Prot
Match: F4KEL0 (Subtilisin-like protease SBT5.5 OS=Arabidopsis thaliana OX=3702 GN=SBT5.5 PE=3 SV=2)

HSP 1 Score: 810.8 bits (2093), Expect = 1.3e-233
Identity = 442/802 (55.11%), Postives = 547/802 (68.20%), Query Frame = 0

Query: 10  LLLLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKDTEEEAKASL 69
           L LLL L+PL ASC+ K+KQ YIVYFGEH+GDKA+ EIEA+HHSYL SVK+TEE+A +SL
Sbjct: 11  LSLLLFLVPLLASCT-KEKQVYIVYFGEHKGDKAFHEIEAHHHSYLQSVKETEEDATSSL 70

Query: 70  LYSYKHSINGFAALLTADEASKLSELDEVVSVIRS--QKYSTHTTRSWEFSGVEE----- 129
           LY    SINGFAA LT D+AS+L EL EVVSV +S  +KY  HTTRSWEF G++E     
Sbjct: 71  LYRRASSINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGLKEEEGED 130

Query: 130 -----DKPR----IND-------LVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPK 189
                D PR    +ND        +  AK+G  V++G++DSGVWP+S SF DKGMGPIP+
Sbjct: 131 YRSDGDAPRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDDKGMGPIPE 190

Query: 190 SWKGICHTGPGFEPSHCNRKIIGARYYLKGYENQFGRLN--ETEDYRSPCDKDGHGSHTA 249
           SWKGIC TG  F  SHCNRKIIGARYY +GYE  +G  N    +D+ SP D DGHGSHTA
Sbjct: 191 SWKGICQTGVAFNSSHCNRKIIGARYYARGYERYYGPFNAEANKDFLSPRDADGHGSHTA 250

Query: 250 SIAGGQRVYNVSAFGGVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAM 309
           S A G+RV  VSA GG+A GTASGGA   RLA+YK CWA+PN+ K   N CFD D LAA 
Sbjct: 251 STAVGRRVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCFDEDMLAAF 310

Query: 310 DDAIADGVDVLSLSIGKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIA 369
           DDAIADGV+V+S+SIG  +P+ Y +DGIAIGALHAVK+DIVV+ SAGN GP    LSN A
Sbjct: 311 DDAIADGVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPARETLSNPA 370

Query: 370 PWIITVGASTLDREFYSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNE 429
           PWIITVGAS+LDR F   + LG+G   +  S+  + LK     PLVYA D++ P V RN+
Sbjct: 371 PWIITVGASSLDRFFVGRLELGDGYVFESDSL--TTLKMDNYAPLVYAPDVVVPGVSRND 430

Query: 430 TGLCVAESLSHEKARGKIVLCYRGVGISRYAG-SLEVKRSGGAGMILGNVPAVGRKLHSD 489
             LC+  +LS +  RGK+VLC RG G     G  LEVKR+GG GMIL N          +
Sbjct: 431 AMLCLPNALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SRDNDAFDVE 490

Query: 490 PHFVPATAVSYEDANIILKYIKSRRNPTATILPPITI-YGSRPAPAMANFSSRGPNLIDP 549
            HFVP   V     + IL YI +   P A I P  T+ Y ++P  ++  FSSR PN +D 
Sbjct: 491 SHFVPTALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPFSSRAPNWVDA 550

Query: 550 HILKPDITAPGVDILAAWSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRA 609
           +ILKPDI APG++ILAAWSG DS +K    +D R++ YNL SGTSMSCPHV+ A ALL++
Sbjct: 551 NILKPDIIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHVAGAIALLKS 610

Query: 610 IHPSWSQAAIRSALMTTATARNKAGMPITEDATDHSATPFSYGSGHFCPSKAADPGLVYD 669
           +HP+WS AAIRSALMTTA+  N+   PI +D     A PF+ GS HF P+KAA PGLVYD
Sbjct: 611 MHPTWSSAAIRSALMTTASMTNEDNEPI-QDYDGSPANPFALGSRHFRPTKAASPGLVYD 670

Query: 670 ADYTDYLHYLCGLGMNSIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV--- 729
           A Y  YL Y C +G+ ++DPTFKCP+R  P  +LNYPSI++P L G V + RTVT V   
Sbjct: 671 ASYQSYLLYCCSVGLTNLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVTRTVTCVGRT 730

Query: 730 GGSRSVYLFYSRPPPGVIVRASPSILYFRGVGEKKSFTI-------TVSGKAKIGVDSSG 775
           G S SVY+F ++PP GV+V+A P++L F  +G+KK F I         +G+A+       
Sbjct: 731 GNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGEAR----RDR 790

BLAST of Sgr019189 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 1.6e-178
Identity = 359/782 (45.91%), Postives = 474/782 (60.61%), Query Frame = 0

Query: 6   FVCLLLLLLLLLPLQASCSDKQKQAYIVYFGEHR-----GDKAWQEIEANHHSYLLSVKD 65
           F  LLLLLL+ +  +   + K   +Y+VYFG H       + A   ++  H+ +L S   
Sbjct: 7   FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66

Query: 66  TEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGV 125
           + E A  ++ YSY   INGFAA L  D A ++S+  EVVSV  ++    HTTRSW+F G+
Sbjct: 67  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126

Query: 126 EEDK-PRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFE 185
           E +     + +  +A++G+D +I  LD+GVWP+S+SF D+G+GPIP  WKGIC       
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKD-A 186

Query: 186 PSHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFG 245
             HCNRK+IGARY+ KGY    G LN + D  SP D DGHGSHT S A G  V  VS F 
Sbjct: 187 TFHCNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFVPGVSIF- 246

Query: 246 GVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSI 305
           G   GTA GG+P  R+A YKVCW       + GN C+D D LAA D AI DG DV+S+S+
Sbjct: 247 GQGNGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGADVISVSL 306

Query: 306 GKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREF 365
           G E P ++ +D +AIG+ HA KK IVV CSAGN GP  S +SN+APW ITVGAST+DREF
Sbjct: 307 GGE-PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREF 366

Query: 366 YSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKAR 425
            S ++LGNG   KG S++ + L   K YP++ + +    +    +  LC   SL   K +
Sbjct: 367 ASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTK 426

Query: 426 GKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANI 485
           GKI++C RG    R      V   GG GM+L N    G  L +DPH +PAT ++ +D+  
Sbjct: 427 GKILVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFA 486

Query: 486 ILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAA 545
           + +YI   + P A I P  T  G +PAP MA+FSS+GP+++ P ILKPDITAPGV ++AA
Sbjct: 487 VSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAA 546

Query: 546 WSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTT 605
           ++G  SPT   +  DPR + +N  SGTSMSCPH+S  A LL+  +PSWS AAIRSA+MTT
Sbjct: 547 YTGAVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTT 606

Query: 606 ATARNKAGMPITEDATDHSATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMNS 665
           AT  +    PI ++AT+  ATPFS+G+GH  P+ A +PGLVYD    DYL++LC LG N+
Sbjct: 607 ATIMDDIPGPI-QNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNA 666

Query: 666 ID------PTFKCPARALPAQDLNYPSIAVPKLRGA-VRIRRTVTNVGGSRSVYLFYSRP 725
                     F C +  +   +LNYPSI VP L  + V + RTV NV G  S+Y      
Sbjct: 667 SQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNV-GRPSMYTVKVNN 726

Query: 726 PPGVIVRASPSILYFRGVGEKKSF-TITVSGKAKIGVDSSGYSFGWYAWSDGIHHVRSPI 774
           P GV V   P+ L F  VGE+K+F  I V  K  +   + GY FG   WSD  H VRSPI
Sbjct: 727 PQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNV---AKGYVFGELVWSDKKHRVRSPI 770

BLAST of Sgr019189 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 3.4e-173
Identity = 354/777 (45.56%), Postives = 475/777 (61.13%), Query Frame = 0

Query: 12  LLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKDTEEEAKASLLY 71
           LLL L     S S   +  YIV+  + +   ++      + S L S+ D+     A LLY
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS-----AELLY 72

Query: 72  SYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGVEEDKPRINDLV 131
           +Y+++I+GF+  LT +EA  L     V+SV+   +Y  HTTR+  F G++E      DL 
Sbjct: 73  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE---HTADLF 132

Query: 132 SRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFEPSHCNRKIIGAR 191
             A    DVV+G+LD+GVWP+S+S+SD+G GPIP SWKG C  G  F  S CNRK+IGAR
Sbjct: 133 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 192

Query: 192 YYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFGGVAWGTASGGAP 251
           ++ +GYE+  G ++E+++ RSP D DGHG+HT+S A G  V   S   G A GTA G AP
Sbjct: 193 FFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL-GYASGTARGMAP 252

Query: 252 SVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSIGKEDPYNYTDDG 311
             R+A+YKVCW        LG  CF +D LAA+D AIAD V+VLS+S+G     +Y  DG
Sbjct: 253 RARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVLSMSLG-GGMSDYYRDG 312

Query: 312 IAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREFYSPVILGNGLKI 371
           +AIGA  A+++ I+VSCSAGN GP+ S+LSN+APWI TVGA TLDR+F +  ILGNG   
Sbjct: 313 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 372

Query: 372 KGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKARGKIVLCYRGVGI 431
            G S+   +    K+ P +YAG+            LC+  +L  EK +GKIV+C RG+  
Sbjct: 373 TGVSLFKGEALPDKLLPFIYAGNASN----ATNGNLCMTGTLIPEKVKGKIVMCDRGINA 432

Query: 432 SRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANIILKYIKSRRNPT 491
               G + VK +GG GMIL N  A G +L +D H +PAT V  +  +II  Y+ +  NPT
Sbjct: 433 RVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 492

Query: 492 ATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAAWSGEDSPTKLPK 551
           A+I    T+ G +P+P +A FSSRGPN I P+ILKPD+ APGV+ILAAW+G   PT L  
Sbjct: 493 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 552

Query: 552 YLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTATARNKAGMPIT 611
             D R V++N+ SGTSMSCPHVS  AALL+++HP WS AAIRSALMTTA    K G P+ 
Sbjct: 553 --DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLL 612

Query: 612 EDATDHSATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMNSID------PTFK 671
           + AT   +TPF +G+GH  P+ A +PGL+YD    DYL +LC L   S          + 
Sbjct: 613 DIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYT 672

Query: 672 C-PARALPAQDLNYPSIAV-PKLRGAVRIRRTVTNVGGSRSVYLFYSRPPPGVIVRASPS 731
           C P+++    DLNYPS AV     GA +  RTVT+VGG+ +  +  +    GV +   P+
Sbjct: 673 CDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 732

Query: 732 ILYFRGVGEKKSFTITVSGKAKIGVDSS----GYSFGWYAWSDGIHHVRSPIAVSST 777
           +L F+   EKKS+T+T +      VDSS      SFG   WSDG H V SP+A+S T
Sbjct: 733 VLNFKEANEKKSYTVTFT------VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Sgr019189 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.9e-168
Identity = 345/774 (44.57%), Postives = 456/774 (58.91%), Query Frame = 0

Query: 13  LLLLLPLQASCSDKQKQAYIVYFGEHR-----GDKAWQEIEANHHSYLLSVKDTEEEAKA 72
           LLLL+ L  S +   K++YIVY G H             +  +H ++L S   + E AK 
Sbjct: 24  LLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKE 83

Query: 73  SLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGVEED-KPR 132
           ++ YSYK  INGFAA+L  +EA+++++  +VVSV  ++    HTT SW F  + ++    
Sbjct: 84  AIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVH 143

Query: 133 INDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFEPSHCNRK 192
            + L ++A YG+D +I  LD+GVWP+S+SFSD+G G +P  WKG CH     +   CNRK
Sbjct: 144 KSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-----KDVPCNRK 203

Query: 193 IIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFGGVAWGTA 252
           +IGARY+ KGY    G L     Y +  D DGHGSHT S A G  V   + F G+  GTA
Sbjct: 204 LIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF-GIGNGTA 263

Query: 253 SGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSIGKEDPYN 312
           SGG+P  R+A YKVCW       + G  CFD D LAA++ AI DGVDVLS S+G  D  +
Sbjct: 264 SGGSPKARVAAYKVCWP-----PVDGAECFDADILAAIEAAIEDGVDVLSASVG-GDAGD 323

Query: 313 YTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREFYSPVILG 372
           Y  DGIAIG+ HAVK  + V CSAGN GP    +SN+APW+ITVGAS++DREF + V L 
Sbjct: 324 YMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELK 383

Query: 373 NGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKARGKIVLCY 432
           NG   KG S++   L  +KMY L+ A D    +    +  LC   SL  +K +GKI++C 
Sbjct: 384 NGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCL 443

Query: 433 RGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANIILKYIKS 492
           RG   +R    ++   +G AGM+L N  A G ++ SD H +PA+ + Y+D   +  Y+ S
Sbjct: 444 RGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSS 503

Query: 493 RRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAAWSGEDSP 552
            ++P   I  P     ++PAP MA+FSSRGPN I P ILKPDITAPGV+I+AA++    P
Sbjct: 504 TKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGP 563

Query: 553 TKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTATARNKA 612
           T L    D R   +N  SGTSMSCPH+S    LL+ +HP WS AAIRSA+MTT+  RN  
Sbjct: 564 TDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNR 623

Query: 613 GMPITEDATDHSATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMNSI------ 672
             P+  D +   A PFSYGSGH  P+KAA PGLVYD    DYL +LC +G N+       
Sbjct: 624 RKPMV-DESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFA 683

Query: 673 -DPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNVGGSRSVYLFYSRPPPGVIVR 732
            DP + C  +     D NYPSI VP L G++ + R + NV G  + Y    R P GV V 
Sbjct: 684 EDPQYTC-RQGANLLDFNYPSITVPNLTGSITVTRKLKNV-GPPATYNARFREPLGVRVS 743

Query: 733 ASPSILYFRGVGEKKSFTITVSGKAKIGVDSSGYSFGWYAWSDGIHHVRSPIAV 774
             P  L F   GE K F +T+     + V  SGY FG   W+D  H+VRSPI V
Sbjct: 744 VEPKQLTFNKTGEVKIFQMTL---RPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774

BLAST of Sgr019189 vs. ExPASy TrEMBL
Match: A0A6J1CJX5 (subtilisin-like protease SBT5.6 OS=Momordica charantia OX=3673 GN=LOC111012300 PE=3 SV=1)

HSP 1 Score: 1326.2 bits (3431), Expect = 0.0e+00
Identity = 665/785 (84.71%), Postives = 713/785 (90.83%), Query Frame = 0

Query: 1   MEK-STFVCLLLLLLLLLPLQASCSDKQKQ-AYIVYFGEHRGDKAWQEIEANHHSYLLSV 60
           MEK STFVCLLLLLLLLL  QAS S    Q AYIVYFGEHRGDKAW EIEA HHSYL SV
Sbjct: 32  MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 91

Query: 61  KDTEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFS 120
           KDTEEEA++SL+YSYKHSINGFAA+L+++EASKLSE+D VV VIRS+KYST TTRSWEFS
Sbjct: 92  KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVRVIRSEKYSTQTTRSWEFS 151

Query: 121 GVEEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGF 180
           GVEEDKPR+NDLVSRAK+GKDVVIGMLDSGVWPKS+SFSDKGMGPIPKSW+GIC TGP F
Sbjct: 152 GVEEDKPRLNDLVSRAKFGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWRGICQTGPAF 211

Query: 181 EPSHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAF 240
           + +HCNRKIIGARYYLKGYEN FGRLNETEDYRSPCDKDGHGSHTAS AGG+RV+NVSAF
Sbjct: 212 QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF 271

Query: 241 GGVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLS 300
           GGVA GTASGGAP  RLAIYKVCWAIP QMKILGNVCFDTD LAAMDDAIADGVDVLSLS
Sbjct: 272 GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS 331

Query: 301 IGKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDRE 360
           IGK DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSN APWIITVGAST+DRE
Sbjct: 332 IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE 391

Query: 361 FYSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKA 420
           FYSPVILGNG KI+G SVAPS LKRKKMYPLVYAGDIMKPHVP+N++G+CVA SLSHEKA
Sbjct: 392 FYSPVILGNGFKIRGLSVAPSMLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA 451

Query: 421 RGKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDAN 480
           RGKIVLCYR  GISRYAGSLEVKRSGGAGMILGNVPAVGRKLH+DPHFVPATAVSYEDAN
Sbjct: 452 RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN 511

Query: 481 IILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILA 540
           IILKYIKSR NPTATI+PPITIYGSRPAPAMANFSSRGPN IDPHILKPDITAPGVDILA
Sbjct: 512 IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA 571

Query: 541 AWSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMT 600
           AWSGEDSPTKLPK LDPR+V YNLYSGTSMSCPHV+AAAALLRAIHP+WSQAAIRSALMT
Sbjct: 572 AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT 631

Query: 601 TATARNKAGMPITEDATDHS--ATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLG 660
           TAT  NK G PIT+DA+DHS  ATPFSYGSGHF PSKAADPGLVYDA+YTDYL +LC L 
Sbjct: 632 TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK 691

Query: 661 M-NSIDPTFKCPARALPAQDLNYPSIAVPKLR----GAVRIRRTVTNVGGSRSVYLFYSR 720
           M +SIDP+F CPARA  A DLNYPS+AVP+LR    G VR+ RTV NVGGS+S Y F + 
Sbjct: 692 MAHSIDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGGVRVVRTVRNVGGSKSAYFFRAS 751

Query: 721 PPPGVIVRASPSILYFRGVGEKKSFTITVSGKAKI-GVDSSGYSFGWYAWSDGIHHVRSP 776
            P GV VRASPS+LYF  +G++K FTIT+SGKA+I GVD SGYSFGW+AW+DGIH VRSP
Sbjct: 752 APAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSP 811

BLAST of Sgr019189 vs. ExPASy TrEMBL
Match: A0A0A0KHZ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520430 PE=3 SV=1)

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 646/791 (81.67%), Postives = 707/791 (89.38%), Query Frame = 0

Query: 1   MEKSTFVC----LLLLLLLLLPL----QASCSDKQKQAYIVYFGEHRGDKAWQEIEANHH 60
           MEKS+F+C    LLLLLLLL+ L     AS S+ QK+AYIVYFGEH G+K+ +EI+  HH
Sbjct: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60

Query: 61  SYLLSVKDTEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTT 120
           SYL+ VK++EE+AK+ LLY+YKHSIN FAA+LT  +ASKLS+LDEVVSVI S+KY   TT
Sbjct: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120

Query: 121 RSWEFSGVEEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKP INDLVSRA YGKDVVIGMLDSGVWPKS+SFSDKGMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 HTGPGFEPSHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRV 240
            TGP F+ +HCNRKIIGARYYLKGYE+ FGRLN+T DYRSPCDKDGHGSHTASIAGG+RV
Sbjct: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGV 300
           YNVSAFGGVAWGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD LAAMDDAIADGV
Sbjct: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300

Query: 301 DVLSLSIGKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGA 360
           DVLSLSIGK +PYNYTDDG+AIGALHAVKKDIVVSCSAGNYGPTPSALSN+APWIITVGA
Sbjct: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360

Query: 361 STLDREFYSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAES 420
           ST+DREFYSPVILGNGLKIKG SVAPSKL+RKKMYPLVYAGDIM PH PRN++GLCVA S
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420

Query: 421 LSHEKARGKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAV 480
           LSHEKA+GKIVLC+RG GISR+AGSLEV+RSGGAGMILGNVPAVGR+ H+DPHFVPATAV
Sbjct: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480

Query: 481 SYEDANIILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAP 540
           SYEDANIILKYIKSR+NPTATI+PP+TIYGSRPAPAMANFSSRGPN IDPH LKPDITAP
Sbjct: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540

Query: 541 GVDILAAWSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAI 600
           GVDILAAWS +DSPTKLPKYLDPRIV+YNLYSGTSMSCPHVSAAAALLRAIHP+WSQAAI
Sbjct: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600

Query: 601 RSALMTTATARNKAGMPITEDAT-DHS-ATPFSYGSGHFCPSKAADPGLVYDADYTDYLH 660
           RSALMTT+T  NK G PIT+D+T D+S ATPFS+GSGHF PSKAADPGLVYD++YTDYLH
Sbjct: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660

Query: 661 YLCGLGMNSIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV-GGSRSVYLFY 720
           YLCGL MNSIDP+FKCP RAL   DLNYPSIAVP+LR  VRI+RTVTNV GG ++VY F 
Sbjct: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720

Query: 721 SRPPPGVIVRASPSILYFRGVGEKKSFTITVSGKAKIGVDSS----GYSFGWYAWSDGIH 777
           S  P GV V ASP+ILYF  VGE+K FTIT+S K      SS     YSFGW+AWSDGIH
Sbjct: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780

BLAST of Sgr019189 vs. ExPASy TrEMBL
Match: A0A6J1EMK8 (subtilisin-like protease SBT5.6 OS=Cucurbita moschata OX=3662 GN=LOC111433935 PE=3 SV=1)

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 643/779 (82.54%), Postives = 702/779 (90.12%), Query Frame = 0

Query: 1   MEKSTFVCLLLLLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKD 60
           ME S+  CLLLLLLLL P  +SC   QKQAYIVYFGEH GDK+W+EIE  HHSYL+SVKD
Sbjct: 1   MEISSLFCLLLLLLLLPP--SSCL-AQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKD 60

Query: 61  TEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGV 120
           TE +AK+SLLYSYKH+INGFAA+LT  +ASKLSEL+EVVSVI S+KY  HTTRSWEFSGV
Sbjct: 61  TENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTTRSWEFSGV 120

Query: 121 EEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFEP 180
           EEDKPR++DLVS+AK GKDVVIGMLDSGVWPKS+SFSDKGMGP+PKSWKGIC TGP FE 
Sbjct: 121 EEDKPRLSDLVSKAKAGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPAFES 180

Query: 181 SHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFGG 240
           SHCNRKIIGARYY+KGYE+ FGRLNET D+RSPCD DGHGSHTASIAGG+RVYNVSAFGG
Sbjct: 181 SHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGG 240

Query: 241 VAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSIG 300
           VA GTASGGAP VRLAIYKVCWAIPNQMK LGNVCFDTD LAAMDDAI+DGVDVLSLSIG
Sbjct: 241 VARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIG 300

Query: 301 KEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREFY 360
           K DPYNYTDDGIAIGALHAVK+DIVVSCSAGN GPTPS LSN+APWIITVGAST+DREFY
Sbjct: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFY 360

Query: 361 SPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKARG 420
           SPVILGNGLKIKG SVAPSKL+R KMYPLVYA DI KPHVPRNE+G C+A SLSH+KA+G
Sbjct: 361 SPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKG 420

Query: 421 KIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANII 480
           KIVLCYRG GISRYAGSLEVKRSGGAGMILG VPAVG +LH+DPHFVPATAVSY+DAN I
Sbjct: 421 KIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRI 480

Query: 481 LKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAAW 540
           LKYIKS RNPTATI+PPITIYGSRPAPAMANF+SRGP+L+DPH LKPDITAPGVDILAAW
Sbjct: 481 LKYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAW 540

Query: 541 SGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTA 600
           S +DSPTKLPK  DPRIVKYN+YSGTSMSCPHVSAAAALLRAIHP+WSQAAIRSALMTTA
Sbjct: 541 SEKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTA 600

Query: 601 TARNKAGMPITEDA-TDHS-ATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMN 660
           T  NK G PIT+D+ TD+S ATPFS+GSGHF P+KAADPGLVYDA+YTDYLHYLCGL MN
Sbjct: 601 TTTNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV-GGSRSVYLFYSRPPPGVI 720
           SID +FKCP RAL   DLNYPSIA+PKL+G VRI+RTVTNV GG +SVY F S  PPGV 
Sbjct: 661 SIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGVA 720

Query: 721 VRASPSILYFRGVGEKKSFTITVSGKAKIGVDSSGYSFGWYAWSDGIHHVRSPIAVSST 777
           VRASPS+LYF  VG++K FTITVSGK K     +GYSFGW+AWSDG+H+VRSPIA+SST
Sbjct: 721 VRASPSVLYFDRVGQRKRFTITVSGKVK----GNGYSFGWFAWSDGVHYVRSPIAISST 772

BLAST of Sgr019189 vs. ExPASy TrEMBL
Match: A0A6J1KU93 (subtilisin-like protease SBT5.6 OS=Cucurbita maxima OX=3661 GN=LOC111497250 PE=3 SV=1)

HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 638/779 (81.90%), Postives = 698/779 (89.60%), Query Frame = 0

Query: 1   MEKSTFVCLLLLLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKD 60
           ME ST  CLLLLLLL     +SC   QKQAYIVYFGEH GDK+W+EIE  HHSYL+SVKD
Sbjct: 1   METSTLFCLLLLLLL---PPSSCL-AQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKD 60

Query: 61  TEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGV 120
           TE +AK+SLLYSYKH+INGFAA+LT  +ASKLSEL+EVVSVI S+ Y  HTTRSWEFSGV
Sbjct: 61  TENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKTYGMHTTRSWEFSGV 120

Query: 121 EEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFEP 180
            EDKPR++DLVS+AK GKDVVIGMLDSGVWPKS+SFSD GMGP+PKSWKGIC TGPGFE 
Sbjct: 121 VEDKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDNGMGPVPKSWKGICQTGPGFES 180

Query: 181 SHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFGG 240
            HCNRKIIGARYY+KGYE+ FGRLNET D+RSPCD DGHGSHTASIAGG+RVYNVSAFGG
Sbjct: 181 PHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGG 240

Query: 241 VAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSIG 300
           VA GTASGGAP VRLAIYKVCWAIPNQMK LGNVCFDTD LAAMDDAI+DGVDVLSLSIG
Sbjct: 241 VARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIG 300

Query: 301 KEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREFY 360
           K DPYNYTDDGIAIGALHAVK+DIVVSCSAGN GPTPS LSN+APWIITVGAST+DREFY
Sbjct: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFY 360

Query: 361 SPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKARG 420
           SPVILGNGLKIKG SVAPSKL+R KMYPLVYA DI KPHVP+NE+G C+A SLSH+KA+G
Sbjct: 361 SPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPQNESGSCIAGSLSHKKAKG 420

Query: 421 KIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANII 480
           KIVLCYRG GISRYAGSLEVKRSGGAGMILG VPAVG +LH+DPHFVPATAVSY+DAN I
Sbjct: 421 KIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRI 480

Query: 481 LKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAAW 540
           LKYIKS RNPTATI+PPITIYGSRPAPAMANF+SRGP+L+DPH LKPDITAPGVDILAAW
Sbjct: 481 LKYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAW 540

Query: 541 SGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTA 600
           S +DSPT LPK+LDPRIVKYN+YSGTSMSCPHVSAAAALLRAIHP+WSQAAIRSALMTT+
Sbjct: 541 SEKDSPTXLPKFLDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTS 600

Query: 601 TARNKAGMPITEDAT-DHS-ATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMN 660
           T  NK G PIT+D+T D+S ATPFS+GSGHF P+KAADPGLVYDA+YTDYLHYLCGL MN
Sbjct: 601 TTTNKYGHPITDDSTSDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV-GGSRSVYLFYSRPPPGVI 720
           SID +FKCP RAL   DLNYPSIA+PKL+G VRI+RTVTNV GG +SVY F S  PPGV 
Sbjct: 661 SIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGVA 720

Query: 721 VRASPSILYFRGVGEKKSFTITVSGKAKIGVDSSGYSFGWYAWSDGIHHVRSPIAVSST 777
           VRASPS+LYF  VG++K FT+TVSGK K     SGYSFGW+AWSDGIH+VRSPIA+SST
Sbjct: 721 VRASPSVLYFDRVGQRKRFTVTVSGKVK----GSGYSFGWFAWSDGIHYVRSPIAISST 771

BLAST of Sgr019189 vs. ExPASy TrEMBL
Match: A0A1S3B024 (LOW QUALITY PROTEIN: subtilisin-like protease SBT5.6 OS=Cucumis melo OX=3656 GN=LOC103484415 PE=3 SV=1)

HSP 1 Score: 1283.5 bits (3320), Expect = 0.0e+00
Identity = 640/793 (80.71%), Postives = 699/793 (88.15%), Query Frame = 0

Query: 1   MEKSTFVC----LLLLLLLLL-----PLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANH 60
           MEKS+F+C    LLLLLLLLL        AS S+ QK+AYIVYFGEH G+K+ +EI+  H
Sbjct: 1   MEKSSFLCHCHVLLLLLLLLLIGPFIQQAASSSNNQKKAYIVYFGEHHGEKSVEEIKERH 60

Query: 61  HSYLLSVKDTEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHT 120
           HSYL+ VK++EE+AK+ LLY+YKHSIN FAA+LT  +ASKLSELDEVVSVI S+KY   T
Sbjct: 61  HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMET 120

Query: 121 TRSWEFSGVEEDKPRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGI 180
           TRSWEFSGVEEDKP INDLVSRA YGKDVVIGMLDSGVWP S+SFSDKGMGP+PKSWKGI
Sbjct: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPNSKSFSDKGMGPLPKSWKGI 180

Query: 181 CHTGPGFEPSHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQR 240
           C TGP F+ +HCNRKIIGARYYLKGYE+ FGRLNET DYRSPCDKDGHGSHTASIAGG+R
Sbjct: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR 240

Query: 241 VYNVSAFGGVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADG 300
           VYNVSAFGGVAWGTASGGAP  RLAIYKVCWAIPNQMK LGNVCFDTD LAAMDDAIADG
Sbjct: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300

Query: 301 VDVLSLSIGKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVG 360
           VDVLSLSIGK +PYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSN+APWIITVG
Sbjct: 301 VDVLSLSIGKSEPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360

Query: 361 ASTLDREFYSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAE 420
           AST+DREFYSPVILGNGLKIKG SVAPSKL+RKKMYPLVYAGDIM PH PRN++GLCVA 
Sbjct: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420

Query: 421 SLSHEKARGKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATA 480
           SLSHEKA+GK VLC+RG GISR+AGSLEV+RSGGAGMILGNVPAVGR+ H+DPHFVPATA
Sbjct: 421 SLSHEKAKGKXVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480

Query: 481 VSYEDANIILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITA 540
           VSYEDAN+ILKYIKSR NPTATI+PP+TIYGSRPAPAMANFSSRGPN IDPH LKPDITA
Sbjct: 481 VSYEDANVILKYIKSRTNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540

Query: 541 PGVDILAAWSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAA 600
           PGVDILAAWS +DSPTKL KYLDPRIV+YNLYSGTSMSCPHVSAAAALLRAIHP+WSQAA
Sbjct: 541 PGVDILAAWSEQDSPTKLSKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600

Query: 601 IRSALMTTATARNKAGMPITEDAT-DHS-ATPFSYGSGHFCPSKAADPGLVYDADYTDYL 660
           IRSALMTT+T  NK+G PIT+D+T D+S ATPFS+GSGHF PSKAADPGLVYD++YTDYL
Sbjct: 601 IRSALMTTSTTNNKSGQPITDDSTPDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660

Query: 661 HYLCGLGMNSIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV-GGSRSVYLF 720
           HYLCGL MNSIDP+F CP R L   DLNYPSIAVP+LR  VRI+RTVTNV GG +SVY F
Sbjct: 661 HYLCGLKMNSIDPSFTCPPRPLHPHDLNYPSIAVPQLRDVVRIKRTVTNVGGGGKSVYFF 720

Query: 721 YSRPPPGVIVRASPSILYFRGVGEKKSFTITVSGKAKIGVDS------SGYSFGWYAWSD 776
            S  PPGV V ASP+ILYF  VGE+K FTIT+S K      S        YSFGW+AWSD
Sbjct: 721 KSEAPPGVAVSASPNILYFNRVGERKKFTITISRKVNSSSSSRRSKKGEEYSFGWFAWSD 780

BLAST of Sgr019189 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 889.0 bits (2296), Expect = 2.7e-258
Identity = 460/790 (58.23%), Postives = 569/790 (72.03%), Query Frame = 0

Query: 10  LLLLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKDTEEEAKASL 69
           L  LL L+PL ASC++ +KQ YIVYFGEH+GDKA+ EIE +HHSYL SVK++EE+A+ASL
Sbjct: 7   LFPLLFLIPLLASCAE-EKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASL 66

Query: 70  LYSYKHSINGFAALLTADEASKLSELDEVVSVIRS--QKYSTHTTRSWEFSGVEEDKP-- 129
           LYSYKHSINGFAA LT D+ASKL +L EVVSV +S  +KY  HTTRSWEF G+EE++   
Sbjct: 67  LYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDS 126

Query: 130 ----RIND----------LVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGI 189
               R ND           + +AK+G  +++G+LDSGVWP+S+SF+DKGMGP+PKSWKGI
Sbjct: 127 DVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGI 186

Query: 190 CHTGPGFEPSHCNRKIIGARYYLKGYENQFGRLNET--EDYRSPCDKDGHGSHTASIAGG 249
           C TG  F  SHCNRKIIGARYY+KGYE  +G  N T  +D+ SP D DGHGSHTAS A G
Sbjct: 187 CQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVG 246

Query: 250 QRVYNVSAFGGVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIA 309
           +RV   SA GG A G+ASGGAP  RLAIYK CWA PN  K+ GN+C + D LAA+DDAIA
Sbjct: 247 RRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIA 306

Query: 310 DGVDVLSLSIGKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIIT 369
           DGV V+S+SIG  +P+ +T DGIA+GALHAVK++IVV+ SAGN GP P  LSN+APWIIT
Sbjct: 307 DGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIIT 366

Query: 370 VGASTLDREFYSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCV 429
           VGASTLDR F   ++LGNG  IK  S+  +  K  K  PLVYA +++ P +  NET  C+
Sbjct: 367 VGASTLDRAFVGGLVLGNGYTIKTDSI--TAFKMDKFAPLVYASNVVVPGIALNETSQCL 426

Query: 430 AESLSHEKARGKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPA 489
             SL  E   GK+VLC RG G SR    +EVKR+GGAGMILGN+ A G ++ SD HFVP 
Sbjct: 427 PNSLKPELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPT 486

Query: 490 TAVSYEDANIILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDI 549
             V+    + IL+YIK+ +NP A I P  T+Y  + AP+M  FSSRGPN++DP+ILKPDI
Sbjct: 487 AGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDI 546

Query: 550 TAPGVDILAAWSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQ 609
           TAPG+ ILAAWSG DSP+K+   +D R+  YN+YSGTSMSCPHV+ A ALL+AIHP WS 
Sbjct: 547 TAPGLYILAAWSGADSPSKMS--VDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSS 606

Query: 610 AAIRSALMTTATARNKAGMPITEDATDHSATPFSYGSGHFCPSKAADPGLVYDADYTDYL 669
           AAIRSALMTTA   N    PI +D T   A PF+ GSGHF P+KAADPGLVYDA Y  YL
Sbjct: 607 AAIRSALMTTAWMTNDKKKPI-QDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYL 666

Query: 670 HYLCGLGMNSIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNV--GGSRSVYL 729
            Y C + + +IDPTFKCP++  P  + NYPSIAVP L+  V ++RTVTNV  G S S YL
Sbjct: 667 LYGCSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYL 726

Query: 730 FYSRPPPGVIVRASPSILYFRGVGEKKSFTITV---SGKAKIGVDSSGYSFGWYAWSDGI 775
           F  +PP G+ V+A P+IL F  +G+K+ F I +     +     +   Y FGW++W+D +
Sbjct: 727 FSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKV 786

BLAST of Sgr019189 vs. TAIR 10
Match: AT5G45640.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 684.9 bits (1766), Expect = 7.7e-197
Identity = 401/811 (49.45%), Postives = 497/811 (61.28%), Query Frame = 0

Query: 10  LLLLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKDTEEEAKASL 69
           L LLL L+PL ASC+ K+KQ                                  E +AS 
Sbjct: 11  LSLLLFLVPLLASCT-KEKQL--------------------------------REERAS- 70

Query: 70  LYSYKHSINGFAALLTADEASKLSELDEVVSVIRS--QKYSTHTTRSWEFSGVEE----- 129
                 SINGFAA LT D+AS+L EL EVVSV +S  +KY  HTTRSWEF G++E     
Sbjct: 71  ------SINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGLKEEEGED 130

Query: 130 -----DKPR----IND-------LVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPK 189
                D PR    +ND        +  AK+G  V++G++DSGVWP+S SF DKGMGPIP+
Sbjct: 131 YRSDGDAPRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDDKGMGPIPE 190

Query: 190 SWKGICHTGPGFEPSHCNRKIIGARYYLKGYENQFGRLN--ETEDYRSPCDKDGHGSHTA 249
           SWKGIC TG  F  SHCN      RYY +GYE  +G  N    +D+ SP D DGHGSHTA
Sbjct: 191 SWKGICQTGVAFNSSHCN------RYYARGYERYYGPFNAEANKDFLSPRDADGHGSHTA 250

Query: 250 SIAGGQRVYNVSAFGGVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAM 309
           S A G+RV  VSA GG+A GTASGGA   RLA+YK CWA+PN+ K   N CFD D LAA 
Sbjct: 251 STAVGRRVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCFDEDMLAAF 310

Query: 310 DDAIADGVDVLSLSIGKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIA 369
           DDAIADGV+V+S+SIG  +P+ Y +DGIAIGALHAVK+DIVV+ SAGN GP    LSN A
Sbjct: 311 DDAIADGVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPARETLSNPA 370

Query: 370 PWIITVGASTLDREFYSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNE 429
           PWIITVGAS+LDR F   + LG+G   +  S+  + LK     PLVYA D++ P V RN+
Sbjct: 371 PWIITVGASSLDRFFVGRLELGDGYVFESDSL--TTLKMDNYAPLVYAPDVVVPGVSRND 430

Query: 430 TGLCVAESLSHEKARGKIVLCYRGVGISRYAG-SLEVKRSGGAGMILGNVPAVGRKLHSD 489
             LC+  +LS +  RGK+VLC RG G     G  LEVKR+GG GMIL N          +
Sbjct: 431 AMLCLPNALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SRDNDAFDVE 490

Query: 490 PHFVPATAVSYEDANIILKYIKSRRNPTATILPPIT----------IYGSRPAPAMANFS 549
            HFVP   V     + IL YI +   P A I P  T          +Y  +PAP M +F 
Sbjct: 491 SHFVPTALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPYKPAPFMTSF- 550

Query: 550 SRGPNLIDPHILKPDITAPGVDILAAWSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHV 609
                        PDI APG++ILAAWSG DS +K    +D R++ YNL SGTSMSCPHV
Sbjct: 551 ------------LPDIIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHV 610

Query: 610 SAAAALLRAIHPSWSQAAIRSALMTTATARNKAGMPITEDATDHSATPFSYGSGHFCPSK 669
           + A ALL+++HP+WS AAIRSALMTTA+  N+   PI +D     A PF+ GS HF P+K
Sbjct: 611 AGAIALLKSMHPTWSSAAIRSALMTTASMTNEDNEPI-QDYDGSPANPFALGSRHFRPTK 670

Query: 670 AADPGLVYDADYTDYLHYLCGLGMNSIDPTFKCPARALPAQDLNYPSIAVPKLRGAVRIR 729
           AA PGLVYDA Y  YL Y C +G+ ++DPTFKCP+R  P  +LNYPSI++P L G V + 
Sbjct: 671 AASPGLVYDASYQSYLLYCCSVGLTNLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVT 730

Query: 730 RTVTNV---GGSRSVYLFYSRPPPGVIVRASPSILYFRGVGEKKSFTI-------TVSGK 775
           RTVT V   G S SVY+F ++PP GV+V+A P++L F  +G+KK F I         +G+
Sbjct: 731 RTVTCVGRTGNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGE 752

BLAST of Sgr019189 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 627.9 bits (1618), Expect = 1.1e-179
Identity = 359/782 (45.91%), Postives = 474/782 (60.61%), Query Frame = 0

Query: 6   FVCLLLLLLLLLPLQASCSDKQKQAYIVYFGEHR-----GDKAWQEIEANHHSYLLSVKD 65
           F  LLLLLL+ +  +   + K   +Y+VYFG H       + A   ++  H+ +L S   
Sbjct: 7   FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66

Query: 66  TEEEAKASLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGV 125
           + E A  ++ YSY   INGFAA L  D A ++S+  EVVSV  ++    HTTRSW+F G+
Sbjct: 67  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126

Query: 126 EEDK-PRINDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFE 185
           E +     + +  +A++G+D +I  LD+GVWP+S+SF D+G+GPIP  WKGIC       
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKD-A 186

Query: 186 PSHCNRKIIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFG 245
             HCNRK+IGARY+ KGY    G LN + D  SP D DGHGSHT S A G  V  VS F 
Sbjct: 187 TFHCNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFVPGVSIF- 246

Query: 246 GVAWGTASGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSI 305
           G   GTA GG+P  R+A YKVCW       + GN C+D D LAA D AI DG DV+S+S+
Sbjct: 247 GQGNGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGADVISVSL 306

Query: 306 GKEDPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREF 365
           G E P ++ +D +AIG+ HA KK IVV CSAGN GP  S +SN+APW ITVGAST+DREF
Sbjct: 307 GGE-PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREF 366

Query: 366 YSPVILGNGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKAR 425
            S ++LGNG   KG S++ + L   K YP++ + +    +    +  LC   SL   K +
Sbjct: 367 ASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTK 426

Query: 426 GKIVLCYRGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANI 485
           GKI++C RG    R      V   GG GM+L N    G  L +DPH +PAT ++ +D+  
Sbjct: 427 GKILVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFA 486

Query: 486 ILKYIKSRRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAA 545
           + +YI   + P A I P  T  G +PAP MA+FSS+GP+++ P ILKPDITAPGV ++AA
Sbjct: 487 VSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAA 546

Query: 546 WSGEDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTT 605
           ++G  SPT   +  DPR + +N  SGTSMSCPH+S  A LL+  +PSWS AAIRSA+MTT
Sbjct: 547 YTGAVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTT 606

Query: 606 ATARNKAGMPITEDATDHSATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMNS 665
           AT  +    PI ++AT+  ATPFS+G+GH  P+ A +PGLVYD    DYL++LC LG N+
Sbjct: 607 ATIMDDIPGPI-QNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNA 666

Query: 666 ID------PTFKCPARALPAQDLNYPSIAVPKLRGA-VRIRRTVTNVGGSRSVYLFYSRP 725
                     F C +  +   +LNYPSI VP L  + V + RTV NV G  S+Y      
Sbjct: 667 SQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNV-GRPSMYTVKVNN 726

Query: 726 PPGVIVRASPSILYFRGVGEKKSF-TITVSGKAKIGVDSSGYSFGWYAWSDGIHHVRSPI 774
           P GV V   P+ L F  VGE+K+F  I V  K  +   + GY FG   WSD  H VRSPI
Sbjct: 727 PQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNV---AKGYVFGELVWSDKKHRVRSPI 770

BLAST of Sgr019189 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 610.1 bits (1572), Expect = 2.4e-174
Identity = 354/777 (45.56%), Postives = 475/777 (61.13%), Query Frame = 0

Query: 12  LLLLLLPLQASCSDKQKQAYIVYFGEHRGDKAWQEIEANHHSYLLSVKDTEEEAKASLLY 71
           LLL L     S S   +  YIV+  + +   ++      + S L S+ D+     A LLY
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS-----AELLY 72

Query: 72  SYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGVEEDKPRINDLV 131
           +Y+++I+GF+  LT +EA  L     V+SV+   +Y  HTTR+  F G++E      DL 
Sbjct: 73  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE---HTADLF 132

Query: 132 SRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFEPSHCNRKIIGAR 191
             A    DVV+G+LD+GVWP+S+S+SD+G GPIP SWKG C  G  F  S CNRK+IGAR
Sbjct: 133 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 192

Query: 192 YYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFGGVAWGTASGGAP 251
           ++ +GYE+  G ++E+++ RSP D DGHG+HT+S A G  V   S   G A GTA G AP
Sbjct: 193 FFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL-GYASGTARGMAP 252

Query: 252 SVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSIGKEDPYNYTDDG 311
             R+A+YKVCW        LG  CF +D LAA+D AIAD V+VLS+S+G     +Y  DG
Sbjct: 253 RARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVLSMSLG-GGMSDYYRDG 312

Query: 312 IAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREFYSPVILGNGLKI 371
           +AIGA  A+++ I+VSCSAGN GP+ S+LSN+APWI TVGA TLDR+F +  ILGNG   
Sbjct: 313 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 372

Query: 372 KGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKARGKIVLCYRGVGI 431
            G S+   +    K+ P +YAG+            LC+  +L  EK +GKIV+C RG+  
Sbjct: 373 TGVSLFKGEALPDKLLPFIYAGNASN----ATNGNLCMTGTLIPEKVKGKIVMCDRGINA 432

Query: 432 SRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANIILKYIKSRRNPT 491
               G + VK +GG GMIL N  A G +L +D H +PAT V  +  +II  Y+ +  NPT
Sbjct: 433 RVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 492

Query: 492 ATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAAWSGEDSPTKLPK 551
           A+I    T+ G +P+P +A FSSRGPN I P+ILKPD+ APGV+ILAAW+G   PT L  
Sbjct: 493 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 552

Query: 552 YLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTATARNKAGMPIT 611
             D R V++N+ SGTSMSCPHVS  AALL+++HP WS AAIRSALMTTA    K G P+ 
Sbjct: 553 --DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLL 612

Query: 612 EDATDHSATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMNSID------PTFK 671
           + AT   +TPF +G+GH  P+ A +PGL+YD    DYL +LC L   S          + 
Sbjct: 613 DIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYT 672

Query: 672 C-PARALPAQDLNYPSIAV-PKLRGAVRIRRTVTNVGGSRSVYLFYSRPPPGVIVRASPS 731
           C P+++    DLNYPS AV     GA +  RTVT+VGG+ +  +  +    GV +   P+
Sbjct: 673 CDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 732

Query: 732 ILYFRGVGEKKSFTITVSGKAKIGVDSS----GYSFGWYAWSDGIHHVRSPIAVSST 777
           +L F+   EKKS+T+T +      VDSS      SFG   WSDG H V SP+A+S T
Sbjct: 733 VLNFKEANEKKSYTVTFT------VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Sgr019189 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 594.3 bits (1531), Expect = 1.4e-169
Identity = 345/774 (44.57%), Postives = 456/774 (58.91%), Query Frame = 0

Query: 13  LLLLLPLQASCSDKQKQAYIVYFGEHR-----GDKAWQEIEANHHSYLLSVKDTEEEAKA 72
           LLLL+ L  S +   K++YIVY G H             +  +H ++L S   + E AK 
Sbjct: 24  LLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKE 83

Query: 73  SLLYSYKHSINGFAALLTADEASKLSELDEVVSVIRSQKYSTHTTRSWEFSGVEED-KPR 132
           ++ YSYK  INGFAA+L  +EA+++++  +VVSV  ++    HTT SW F  + ++    
Sbjct: 84  AIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVH 143

Query: 133 INDLVSRAKYGKDVVIGMLDSGVWPKSESFSDKGMGPIPKSWKGICHTGPGFEPSHCNRK 192
            + L ++A YG+D +I  LD+GVWP+S+SFSD+G G +P  WKG CH     +   CNRK
Sbjct: 144 KSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-----KDVPCNRK 203

Query: 193 IIGARYYLKGYENQFGRLNETEDYRSPCDKDGHGSHTASIAGGQRVYNVSAFGGVAWGTA 252
           +IGARY+ KGY    G L     Y +  D DGHGSHT S A G  V   + F G+  GTA
Sbjct: 204 LIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF-GIGNGTA 263

Query: 253 SGGAPSVRLAIYKVCWAIPNQMKILGNVCFDTDTLAAMDDAIADGVDVLSLSIGKEDPYN 312
           SGG+P  R+A YKVCW       + G  CFD D LAA++ AI DGVDVLS S+G  D  +
Sbjct: 264 SGGSPKARVAAYKVCWP-----PVDGAECFDADILAAIEAAIEDGVDVLSASVG-GDAGD 323

Query: 313 YTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNIAPWIITVGASTLDREFYSPVILG 372
           Y  DGIAIG+ HAVK  + V CSAGN GP    +SN+APW+ITVGAS++DREF + V L 
Sbjct: 324 YMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELK 383

Query: 373 NGLKIKGFSVAPSKLKRKKMYPLVYAGDIMKPHVPRNETGLCVAESLSHEKARGKIVLCY 432
           NG   KG S++   L  +KMY L+ A D    +    +  LC   SL  +K +GKI++C 
Sbjct: 384 NGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCL 443

Query: 433 RGVGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHSDPHFVPATAVSYEDANIILKYIKS 492
           RG   +R    ++   +G AGM+L N  A G ++ SD H +PA+ + Y+D   +  Y+ S
Sbjct: 444 RGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSS 503

Query: 493 RRNPTATILPPITIYGSRPAPAMANFSSRGPNLIDPHILKPDITAPGVDILAAWSGEDSP 552
            ++P   I  P     ++PAP MA+FSSRGPN I P ILKPDITAPGV+I+AA++    P
Sbjct: 504 TKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGP 563

Query: 553 TKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTATARNKA 612
           T L    D R   +N  SGTSMSCPH+S    LL+ +HP WS AAIRSA+MTT+  RN  
Sbjct: 564 TDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNR 623

Query: 613 GMPITEDATDHSATPFSYGSGHFCPSKAADPGLVYDADYTDYLHYLCGLGMNSI------ 672
             P+  D +   A PFSYGSGH  P+KAA PGLVYD    DYL +LC +G N+       
Sbjct: 624 RKPMV-DESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFA 683

Query: 673 -DPTFKCPARALPAQDLNYPSIAVPKLRGAVRIRRTVTNVGGSRSVYLFYSRPPPGVIVR 732
            DP + C  +     D NYPSI VP L G++ + R + NV G  + Y    R P GV V 
Sbjct: 684 EDPQYTC-RQGANLLDFNYPSITVPNLTGSITVTRKLKNV-GPPATYNARFREPLGVRVS 743

Query: 733 ASPSILYFRGVGEKKSFTITVSGKAKIGVDSSGYSFGWYAWSDGIHHVRSPIAV 774
             P  L F   GE K F +T+     + V  SGY FG   W+D  H+VRSPI V
Sbjct: 744 VEPKQLTFNKTGEVKIFQMTL---RPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774