Sgr018975 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr018975
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionDNA-directed RNA polymerases II and V subunit 8A-like
Locationtig00153231: 649164 .. 651991 (-)
RNA-Seq ExpressionSgr018975
SyntenySgr018975
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGAGCTTCTCTTTGATGATATTTTTAAGATTGAGCGATTAAACCCAGATGGTAAAAAGTTCGATAAAGGTGCGTTTATGCAGAATCGGTACTCTTTTTTTTTTTATTTAGACAATATTTTACCACATCTTCCTTGTTCAAGGTTCTTGTCAGCCGACTATGATAGATTTTATTGCCATACATTTGTAAAATCATTGTGTTTCATTAGATTCCTCTATAATCGTGAAGTTAAGTCTTAGAGTCCAGTTTAATCATATTCTTTGTGTTGGTTATGAAAATGTTTTTGAAGCACAGGATACAAGAAAAAGATTTTTACTTGTCTGTTAGTGCCATTGCAGAGTCAGTTTCTACCATTTATTTAGACTTTTGTGGGGATAGTTGCAGTTGCAGAGCCCCTTTTATGGGAGGATACTTGGTTCCGAACAGATAGGATTGAGAAACCTCCTGAATGTATACCATATGTTAGGCAAAGAATGAGAGAGTTAGGTAGGTAGAAAGGACGTGCGCGCTCATCGGTCCTTCAAGTATAGAAATAAGTGGGGATCACCTTTTTTTTGCTGCTTTGAAAATGGAGGAGATGCTAAGTGGGGATCACTTTTGTAATTCTGTTACTAACAAAATGGAAAAACACCGAGAGGAATGTGTGATGTGAGCACATATGTGTGTAATGTAAGTTTAGTTAAGGTCACACTGGAAATTTTAGGAGGGAAAGGGGAGAACAAACCACTTGAAAGTTTGCCACATTTAGTACATTTTCTTATGTGAGTTTTATCATAGTACTACCTAGTCATAGTTTTCCCGTTTATATTCTTTATCTGTTACCAAAAATTATCTGATTCTTTTGGGTTTTTTTATTAGACGGCCAATTTTCCTCTTTTTTTTTTCTCTATAGATTCTTTTTGGTAAGGGATCAATGAGTTAGCGTCCCTCCTATCTATTAACCAAGGAGTGAATCTGATTCGTGGTAATAGGGAACCACATGTATGGTCTCTTGATCCTCTGATGTTTCACTTGTTAGTCTTTGTAGACTCCATTAGTTTCTCCTCCTTCTTTCGTCTCAAAACTTTGGAAGTTGATGATCCTGAAAAATATTTCGGTCTTTTCCAATCTTGTCACCATTGGGAGGATTGACAACTTTGACTTCATACAGAGAGCCTTTACTTTGTCCTTAGCCCCAACTGGTGTATTTGTATAGACACGCTTTGGAACACCTAAACCACCTATTGTATCTTTGCCTTTTTCCTACCAAGGTGTTGTGTCGTTTGTGGCGAACTTTTGGCTTGTCTATGATCCAGAGTTTGAGTATTCGCAGGATGATAGAGAAGTTAATTTGGCTCTTTCATTCCAGGACAAAGGACATTAAATTTTGGCAATATCTTTTTTTTAATTTATTTTTGCTATTGTATGGAATATTTGATTGGAAAGAAACAAGAGAATCTTCTCAGATAGGTCGTGGGAGGAGGTTTGGGGTTGAGACATTTTAGTGTCTTTCTTTGGATTCATACTTTTAGACTCTTTTGTAGTTGGGAACCCTTTGCTTTGGATTACCAATAGCTGGGGATCCTTTTGTAAGTTTTTGTTAGTCCCTGCATTTGCTGACCACTTTTTGTGGCTTTTTTCCGTTGTTTCATTTATTTTGTTAATGATAGCTGGATTTTTTTTTTTAAATCAGTAATGCTATAATAAATGTCTTATTATGAGCCATATGAAATTTGTGAAAAATAAAAACTTTGCAACACATTGAGGCAAATGGCATTTATGTTTTCACAATCTGTCCTACAGTTACTCGAATCGAGGCAAAGAGCGAGAAGTTTGACATGTTTATGCATCTAGATATAAATACAGAAATATACAACTTGAAAGAAGGTGAGAAGTTTTCGATGGCATTGGCTCCAACTCTAAATCTAGATGGAACCCCTGACACAGGCTATTATCTTCAGGTAACTTGTTCTTCCAATGGTTAGTCTGTTGCTCGCTGGATGTAGTGTAGTATTTGTAAGAAAATAATTCCCTGCTACAGCGGTTACTGGCTGACTGAAAAATTTAGTTGTCCATTGAATTATAAATCACTAGTTTTGAAATCTCACTTTGACATTAATAGGTAACAACGAACTTTCAATCAACTAGGAAATATTTGCTTTCTATAGTTATTGCATATGTCTATTTACTGTCATGACTGGTCTTTCGCTGTTTTGTGAGAACTGTATTTGTACCTTCCTCTGTTCAAATATTAATTTGATCTTTGAAATTCTCGAGCCTTGAATATTTATGTCCATTTAATTATCGTTAATCTGTGTAACAGGGCAATCGGAAGTCGCTTGCTGACAGATACGAATATGTCATGCATGGGAAGCTGTACAGAATATCGGAAAGTTCTGGACATGGCGGTAAAGCGTACGTGCTATAACTTCTCAAACTTTTTCAATTTTGAACACTACAATTATATTGTTGAGCCATCAAAGAAGCAAGAGCTTGACATTGCCGAAGTTACATTTTGCTATATTTGAGCAAGAATCTGTCTGAGAGCATTGTCACCTGTCTGTAATGGCTACCTAAATGGCTGTCATGTGGGACAGAATTCATTTTAGTTTCTTTTTCTGAACAGTTTAGGAACTTACATATTTTCTAGTTGAATTATTATCTTTTAAAAAATGTATTTCATCTGAATCCAGGGAAATAAATGTTTCTTTTGGTGGGCTTCTGATGATGCTGAAGGGGGATCCCTCACATTGCAGCAAGTTTGAGCTTGATCAGAGGCTGTTTCTTCTCATCAGGAAGGTGTGAAGCTCCGTCATTTGAATTGAACTCTGATGACTTACGATAA

mRNA sequence

ATGGTTGAGCTTCTCTTTGATGATATTTTTAAGATTGAGCGATTAAACCCAGATGGTAAAAAGTTCGATAAAGTTACTCGAATCGAGGCAAAGAGCGAGAAGTTTGACATGTTTATGCATCTAGATATAAATACAGAAATATACAACTTGAAAGAAGGTGAGAAGTTTTCGATGGCATTGGCTCCAACTCTAAATCTAGATGGAACCCCTGACACAGGCTATTATCTTCAGGGCAATCGGAAGTCGCTTGCTGACAGATACGAATATGTCATGCATGGGAAGCTGTACAGAATATCGGAAAGTTCTGGACATGGCGGTAAAGCGGGGATCCCTCACATTGCAGCAAGTTTGAGCTTGATCAGAGGCTGTTTCTTCTCATCAGGAAGGTGTGAAGCTCCGTCATTTGAATTGAACTCTGATGACTTACGATAA

Coding sequence (CDS)

ATGGTTGAGCTTCTCTTTGATGATATTTTTAAGATTGAGCGATTAAACCCAGATGGTAAAAAGTTCGATAAAGTTACTCGAATCGAGGCAAAGAGCGAGAAGTTTGACATGTTTATGCATCTAGATATAAATACAGAAATATACAACTTGAAAGAAGGTGAGAAGTTTTCGATGGCATTGGCTCCAACTCTAAATCTAGATGGAACCCCTGACACAGGCTATTATCTTCAGGGCAATCGGAAGTCGCTTGCTGACAGATACGAATATGTCATGCATGGGAAGCTGTACAGAATATCGGAAAGTTCTGGACATGGCGGTAAAGCGGGGATCCCTCACATTGCAGCAAGTTTGAGCTTGATCAGAGGCTGTTTCTTCTCATCAGGAAGGTGTGAAGCTCCGTCATTTGAATTGAACTCTGATGACTTACGATAA

Protein sequence

MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMALAPTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGIPHIAASLSLIRGCFFSSGRCEAPSFELNSDDLR
Homology
BLAST of Sgr018975 vs. NCBI nr
Match: XP_022932746.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_022932747.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_023538873.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo] >XP_023538874.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 219.9 bits (559), Expect = 1.4e-53
Identity = 107/110 (97.27%), Postives = 109/110 (99.09%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGI 111
           APTLNLDGTPDTGY+LQGNRKSLADRYEYVMHGKL+RISESSGHGGKA I
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAEI 110

BLAST of Sgr018975 vs. NCBI nr
Match: KAG6597681.1 (DNA-directed RNA polymerases II and V subunit 8A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 219.9 bits (559), Expect = 1.4e-53
Identity = 107/110 (97.27%), Postives = 109/110 (99.09%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL
Sbjct: 178 MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 237

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGI 111
           APTLNLDGTPDTGY+LQGNRKSLADRYEYVMHGKL+RISESSGHGGKA I
Sbjct: 238 APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAEI 287

BLAST of Sgr018975 vs. NCBI nr
Match: KAG7029125.1 (DNA-directed RNA polymerases II and V subunit 8A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 219.5 bits (558), Expect = 1.8e-53
Identity = 106/108 (98.15%), Postives = 108/108 (100.00%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKA 109
           APTLNLDGTPDTGY+LQGNRKSLADRYEYVMHGKL+RISESSGHGGKA
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKA 108

BLAST of Sgr018975 vs. NCBI nr
Match: XP_022145813.1 (DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica charantia] >XP_022145814.1 DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica charantia] >XP_022145815.1 DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica charantia])

HSP 1 Score: 216.9 bits (551), Expect = 1.2e-52
Identity = 105/110 (95.45%), Postives = 108/110 (98.18%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGI 111
           APTLNLDGTPDTGYYLQGNRKSLADRYEY+M GKL+RISESSGHGG+A I
Sbjct: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYIMQGKLFRISESSGHGGRAEI 110

BLAST of Sgr018975 vs. NCBI nr
Match: XP_022972118.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita maxima] >XP_022972119.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita maxima])

HSP 1 Score: 216.5 bits (550), Expect = 1.5e-52
Identity = 106/110 (96.36%), Postives = 108/110 (98.18%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGI 111
           APTLNLDGTPDTGY+LQGNRKSLADRYEYVMHGKL+RISESSGH GKA I
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHVGKAEI 110

BLAST of Sgr018975 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.1e-37
Identity = 68/105 (64.76%), Postives = 90/105 (85.71%), Query Frame = 0

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLD+NTE+Y L  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKA 109
           LNLDGTPDTGY+  G +K+LAD+YEY+MHGKLY+ISE  G   KA
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKA 110

BLAST of Sgr018975 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 2.5e-37
Identity = 69/105 (65.71%), Postives = 89/105 (84.76%), Query Frame = 0

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLD+NTE+Y L  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKA 109
           LNLDGTPDTGY+  G +K+LAD+YEY+MHGKLY+ISE  G   KA
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKA 110

BLAST of Sgr018975 vs. ExPASy Swiss-Prot
Match: Q54YW8 (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 2.7e-20
Identity = 48/122 (39.34%), Postives = 76/122 (62.30%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           M  +LF+D F+++ ++PDGKKFD+V+R    SE ++M + +DI T IY L   E+F M L
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGIPHIAASLSLI 120
           A +LNLDG+ D       N+ SLAD+Y+YVM+GK+++  + +     +        L L+
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENSSSKVSVFASFGGLLVLL 120

Query: 121 RG 123
           +G
Sbjct: 121 QG 121

BLAST of Sgr018975 vs. ExPASy Swiss-Prot
Match: P52434 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=9606 GN=POLR2H PE=1 SV=4)

HSP 1 Score: 99.4 bits (246), Expect = 3.6e-20
Identity = 48/99 (48.48%), Postives = 71/99 (71.72%), Query Frame = 0

Query: 1  MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
          M  +LF+DIF ++ ++P+GKKFD+V+R+  +SE F M + LD+N +IY +  G+KF + +
Sbjct: 1  MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI 60

Query: 61 APTLNLDGTPDTG-YYLQGNRKSLADRYEYVMHGKLYRI 99
          A TL  DGT D G Y    +R S AD++EYVM+GK+YRI
Sbjct: 61 ASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRI 99

BLAST of Sgr018975 vs. ExPASy Swiss-Prot
Match: Q923G2 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Mus musculus OX=10090 GN=Polr2h PE=1 SV=3)

HSP 1 Score: 99.4 bits (246), Expect = 3.6e-20
Identity = 48/99 (48.48%), Postives = 71/99 (71.72%), Query Frame = 0

Query: 1  MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
          M  +LF+DIF ++ ++P+GKKFD+V+R+  +SE F M + LD+N +IY +  G+KF + +
Sbjct: 1  MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI 60

Query: 61 APTLNLDGTPDTG-YYLQGNRKSLADRYEYVMHGKLYRI 99
          A TL  DGT D G Y    +R S AD++EYVM+GK+YRI
Sbjct: 61 ASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRI 99

BLAST of Sgr018975 vs. ExPASy TrEMBL
Match: A0A6J1EXM3 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita moschata OX=3662 GN=LOC111439203 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 6.7e-54
Identity = 107/110 (97.27%), Postives = 109/110 (99.09%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGI 111
           APTLNLDGTPDTGY+LQGNRKSLADRYEYVMHGKL+RISESSGHGGKA I
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAEI 110

BLAST of Sgr018975 vs. ExPASy TrEMBL
Match: A0A6J1CWD5 (DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015176 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 5.7e-53
Identity = 105/110 (95.45%), Postives = 108/110 (98.18%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGI 111
           APTLNLDGTPDTGYYLQGNRKSLADRYEY+M GKL+RISESSGHGG+A I
Sbjct: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYIMQGKLFRISESSGHGGRAEI 110

BLAST of Sgr018975 vs. ExPASy TrEMBL
Match: A0A6J1I8Y6 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita maxima OX=3661 GN=LOC111470753 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 7.4e-53
Identity = 106/110 (96.36%), Postives = 108/110 (98.18%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGI 111
           APTLNLDGTPDTGY+LQGNRKSLADRYEYVMHGKL+RISESSGH GKA I
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHVGKAEI 110

BLAST of Sgr018975 vs. ExPASy TrEMBL
Match: A0A0A0KYV5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.4e-51
Identity = 104/110 (94.55%), Postives = 107/110 (97.27%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGI 111
           APTLNLDGTPDTGY+LQGNRKSLADRYEYVM GKL+RISESSGHGGKA I
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEI 110

BLAST of Sgr018975 vs. ExPASy TrEMBL
Match: A0A1S3BXS2 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN=LOC103494598 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.8e-51
Identity = 103/110 (93.64%), Postives = 107/110 (97.27%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+N+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKAGI 111
           APTLNLDGTPDTGY+LQGNRKSLADRYEYVM GKL+RISESSGHGGKA I
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEI 110

BLAST of Sgr018975 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 157.5 bits (397), Expect = 7.8e-39
Identity = 68/105 (64.76%), Postives = 90/105 (85.71%), Query Frame = 0

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLD+NTE+Y L  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKA 109
           LNLDGTPDTGY+  G +K+LAD+YEY+MHGKLY+ISE  G   KA
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKA 110

BLAST of Sgr018975 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 156.4 bits (394), Expect = 1.7e-38
Identity = 69/105 (65.71%), Postives = 89/105 (84.76%), Query Frame = 0

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLD+NTE+Y L  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYYLQGNRKSLADRYEYVMHGKLYRISESSGHGGKA 109
           LNLDGTPDTGY+  G +K+LAD+YEY+MHGKLY+ISE  G   KA
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKA 110

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022932746.11.4e-5397.27DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_0... [more]
KAG6597681.11.4e-5397.27DNA-directed RNA polymerases II and V subunit 8A, partial [Cucurbita argyrosperm... [more]
KAG7029125.11.8e-5398.15DNA-directed RNA polymerases II and V subunit 8A [Cucurbita argyrosperma subsp. ... [more]
XP_022145813.11.2e-5295.45DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica char... [more]
XP_022972118.11.5e-5296.36DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita maxima] >XP_022... [more]
Match NameE-valueIdentityDescription
Q9M1A81.1e-3764.76DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=... [more]
O810972.5e-3765.71DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702... [more]
Q54YW82.7e-2039.34DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc... [more]
P524343.6e-2048.48DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=96... [more]
Q923G23.6e-2048.48DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Mus musculus OX=10... [more]
Match NameE-valueIdentityDescription
A0A6J1EXM36.7e-5497.27DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita moschata OX=3... [more]
A0A6J1CWD55.7e-5395.45DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 OS=Momordica ch... [more]
A0A6J1I8Y67.4e-5396.36DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita maxima OX=366... [more]
A0A0A0KYV51.4e-5194.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1[more]
A0A1S3BXS21.8e-5193.64DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT3G59600.17.8e-3964.76RNA polymerase Rpb8 [more]
AT1G54250.11.7e-3865.71RNA polymerase Rpb8 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 2..139
e-value: 2.4E-37
score: 140.0
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 7..104
e-value: 4.5E-35
score: 120.8
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..107
NoneNo IPR availableGENE3D2.40.50.140coord: 6..118
e-value: 6.3E-39
score: 135.2
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 4..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr018975.1Sgr018975.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity