Sgr018948 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr018948
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionDelta(3,5)-Delta(2,4)-dienoyl-CoA isomerase
Locationtig00153231: 229546 .. 231353 (+)
RNA-Seq ExpressionSgr018948
SyntenySgr018948
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATTACAAGTTCGTAAAAATCGTTCGCCGGAATCCCAATTCCGCCGTCTTCCACCTCCACCTCCACCGCCCCTCCCATCACAACGCCCTCACGACCGAGCTCTTCTCCGAATTCGGTCGAGCCCTCTCTTCTCTCGACCAAAACCCCGACGTTCGCGTCATCATCCTCTCCGGATCCGGCAAGAACTTCTGCGCCGGAATCGATCTCAAATCCACCAGTTCCAATTTCGAGAAGTTGCTCTCCGGCGACCGCGGCAGGGCCGGAGAGAAGATCCGACGGGAGATCAAGGCCATGCAGGACTCGATCACCGCAATCGAGCAGTGCCGGAAGCCGGTGATTGCGAGCATCCACGGAGGTTGCATCGGCGGAGGGGTTGATATAATCACCGCCTGTGACATAAGGTATTGCACGGAGGACGCGTTCTTCTCAGTGAAGGAGGTTAATTTGGGGATCACCGCCGACCTTGGGACTCTGCAGAGGCTGCCGGCGATCGTTGGGTATGGCAAGGCGGCCGAATTGGCGTTGACGGGTCGAAATTTTTCGGGTTTGGAGGCCAAGGAATTGGGCCTGGTTTCAAGAACCTTCACTTCCATGGAGCTGCTAGAAGACGGAGTGACAAAAATTGCCGAAGGTAATTTCGTCGATTTTAATTTTTAACGTTTCAGTCAATTGAATCATGCATTTGGTCACTACAGAGTTTAGGTTTAATTTTAATTTGATTCTTATAATTTGGGCATAGATTTTAACGTGATCTCCTTTTTTAATATGGTCTCTATCATTTTGTGTGCTGTTGCCCCATCATCACTTTTTTATTTACGTAATAGTAAGTTTGATATTTTTTATTTAGCTGATTATGTTAATTAATTAAATTAATGTTTAAGTCTGAGTGAGTGAAAAAAAAGAGAGAATATGTGAGTTTTCAGGTGGATGAAAAATAGTTTAGGAACGATTTTTAACCAAACTATAAGAACCAAATTGAAACTTGATCTAAATCATAGGGATCAAATTCAGAACTTTAAAATAATATAGACTAAATTGAAATTAAGTTCAAATTATAAAAATTAAATTGATAATTTAATCTTATTGAATCTACATCAGGATATTTTTGTTTAAATTATAAATTTAAACCTTAAAAAAATATTTAATTACGTTCTTAAATTTTCGATTTTTGTTCTAACAAATCTCTATTGATAATACGATTAGTTAAATATTGACGTGAATCAATAGGTGGGAATAATAAAACAAGGAGATGGAGAAAAAAGTCGGTTTAGGATGTATATTTTCTACTTATCCCATCAAATTTTCAACTTGCCCTTATTTGATCCAGTTGGCTTATTTTCTTGTGCCACATCACGGTGTTGAGGATAATAACTTATTAGAATCAGCGTGTTACATCAACATACAATAATAGCTAATATGTTAGATCAATACAGGTAAAAAAAAAAAAGTTCTAACTTATTCTCACTCGGTTGTTCTTTTTCTTTTTTAAATATAGCATTGCAAATGTGGGGTGGAAACACCGTTTTTTTTTAATCACCAAAGCAATCAAAAAACTTGTGGTTATGGATGAAGGAAGAAAGAAAGAGTGAAATTTGATTTTGGTGAATGCAGAAATAGCATCAAAATCTCCGCTGGCTGTGGTTGGGACAAAGGCAGTGTTGCTAAAAAGCAGGGACCTGAACGTAGAACAGGGGTTGGATTATGTTGCGACTTGGAACTCAGGAACCCTCCTCTCAGACGATCTCAAAGAGGCAATCTCTGCCCAAGCTCACAAACGCAACCCTGCTTTTGCCAAGCTCTGA

mRNA sequence

ATGGAAGATTACAAGTTCGTAAAAATCGTTCGCCGGAATCCCAATTCCGCCGTCTTCCACCTCCACCTCCACCGCCCCTCCCATCACAACGCCCTCACGACCGAGCTCTTCTCCGAATTCGGTCGAGCCCTCTCTTCTCTCGACCAAAACCCCGACGTTCGCGTCATCATCCTCTCCGGATCCGGCAAGAACTTCTGCGCCGGAATCGATCTCAAATCCACCAGTTCCAATTTCGAGAAGTTGCTCTCCGGCGACCGCGGCAGGGCCGGAGAGAAGATCCGACGGGAGATCAAGGCCATGCAGGACTCGATCACCGCAATCGAGCAGTGCCGGAAGCCGGTGATTGCGAGCATCCACGGAGGTTGCATCGGCGGAGGGGTTGATATAATCACCGCCTGTGACATAAGGTATTGCACGGAGGACGCGTTCTTCTCAGTGAAGGAGGTTAATTTGGGGATCACCGCCGACCTTGGGACTCTGCAGAGGCTGCCGGCGATCGTTGGGTATGGCAAGGCGGCCGAATTGGCGTTGACGGGTCGAAATTTTTCGGGTTTGGAGGCCAAGGAATTGGGCCTGGTTTCAAGAACCTTCACTTCCATGGAGCTGCTAGAAGACGGAGTGACAAAAATTGCCGAAGAAATAGCATCAAAATCTCCGCTGGCTGTGGTTGGGACAAAGGCAGTGTTGCTAAAAAGCAGGGACCTGAACGTAGAACAGGGGTTGGATTATGTTGCGACTTGGAACTCAGGAACCCTCCTCTCAGACGATCTCAAAGAGGCAATCTCTGCCCAAGCTCACAAACGCAACCCTGCTTTTGCCAAGCTCTGA

Coding sequence (CDS)

ATGGAAGATTACAAGTTCGTAAAAATCGTTCGCCGGAATCCCAATTCCGCCGTCTTCCACCTCCACCTCCACCGCCCCTCCCATCACAACGCCCTCACGACCGAGCTCTTCTCCGAATTCGGTCGAGCCCTCTCTTCTCTCGACCAAAACCCCGACGTTCGCGTCATCATCCTCTCCGGATCCGGCAAGAACTTCTGCGCCGGAATCGATCTCAAATCCACCAGTTCCAATTTCGAGAAGTTGCTCTCCGGCGACCGCGGCAGGGCCGGAGAGAAGATCCGACGGGAGATCAAGGCCATGCAGGACTCGATCACCGCAATCGAGCAGTGCCGGAAGCCGGTGATTGCGAGCATCCACGGAGGTTGCATCGGCGGAGGGGTTGATATAATCACCGCCTGTGACATAAGGTATTGCACGGAGGACGCGTTCTTCTCAGTGAAGGAGGTTAATTTGGGGATCACCGCCGACCTTGGGACTCTGCAGAGGCTGCCGGCGATCGTTGGGTATGGCAAGGCGGCCGAATTGGCGTTGACGGGTCGAAATTTTTCGGGTTTGGAGGCCAAGGAATTGGGCCTGGTTTCAAGAACCTTCACTTCCATGGAGCTGCTAGAAGACGGAGTGACAAAAATTGCCGAAGAAATAGCATCAAAATCTCCGCTGGCTGTGGTTGGGACAAAGGCAGTGTTGCTAAAAAGCAGGGACCTGAACGTAGAACAGGGGTTGGATTATGTTGCGACTTGGAACTCAGGAACCCTCCTCTCAGACGATCTCAAAGAGGCAATCTCTGCCCAAGCTCACAAACGCAACCCTGCTTTTGCCAAGCTCTGA

Protein sequence

MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSGSGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHGGCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGRNFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL
Homology
BLAST of Sgr018948 vs. NCBI nr
Match: XP_022979902.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita maxima])

HSP 1 Score: 459.1 bits (1180), Expect = 2.6e-125
Identity = 232/275 (84.36%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           MEDYK +KI R+NPNS VFHL LHRPSH+N LTTELF E  RALSSLDQNPDVRVIILSG
Sbjct: 1   MEDYKSIKIERQNPNSGVFHLRLHRPSHYNTLTTELFGELRRALSSLDQNPDVRVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFE LLS +RGRAGE++RRE+KAMQDSITAIE+CRKPVIA IHG
Sbjct: 61  SGKHFCAGIDLKSTASNFENLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GCIGGGVD+ TACD+RYCT +A FSVKEVNL ITADLGTLQRLP IVG+GKA ELALTGR
Sbjct: 121 GCIGGGVDMATACDLRYCTAEAVFSVKEVNLAITADLGTLQRLPGIVGFGKAVELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
            FSGLEAKELGLVS TF S   LE+GV KIAEEIASKSPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 EFSGLEAKELGLVSATFASKMELENGVIKIAEEIASKSPLAVVGTKAVLLKSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLS DLKEAISA+AHKRNPAF+KL
Sbjct: 241 LDYVATWNSGTLLSADLKEAISARAHKRNPAFSKL 275

BLAST of Sgr018948 vs. NCBI nr
Match: XP_023526792.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 457.6 bits (1176), Expect = 7.6e-125
Identity = 231/275 (84.00%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           MEDYK +KI R+NP+S VFHL LHRPSH+NALTTELF+E  RALSSLDQNPDVRVIILSG
Sbjct: 1   MEDYKSIKIERQNPSSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFE LLS +RGRAGE++RRE+KAMQDSITAIE+CRKPVIA IHG
Sbjct: 61  SGKHFCAGIDLKSTASNFENLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GCIGGGVDI TACD+RYCT +A FSVKEVNL ITADLGTLQRLP IVG+GKA ELALTGR
Sbjct: 121 GCIGGGVDIATACDLRYCTAEAVFSVKEVNLAITADLGTLQRLPGIVGFGKAVELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
            FSGLEAKELGLVS TF S   L++GV KIAEEIASKSPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 EFSGLEAKELGLVSTTFASKMELDNGVIKIAEEIASKSPLAVVGTKAVLLKSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLS DLKEAISA+AHKRNP F+KL
Sbjct: 241 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

BLAST of Sgr018948 vs. NCBI nr
Match: XP_022956301.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita moschata] >KAG7018444.1 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 457.2 bits (1175), Expect = 9.9e-125
Identity = 230/275 (83.64%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           MEDYK +KI R+NPNS VFHL LHRPSH+NALTTELF+E  RALSSLDQNPDVRVIILSG
Sbjct: 1   MEDYKSIKIERQNPNSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFE LLS +RGRAGE++RRE+KAMQDSITAIE+CRKPVIA IHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEDLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GCIGGGVDI TACD+RYCT +A FSVKE+NL ITADLGTLQRLP IVG+GKA ELALTGR
Sbjct: 121 GCIGGGVDIATACDLRYCTAEAVFSVKEINLAITADLGTLQRLPGIVGFGKAVELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
            FSG EAKELGLVS TF S   L++GV KIAEEIASKSPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 EFSGFEAKELGLVSTTFASKMELDNGVIKIAEEIASKSPLAVVGTKAVLLKSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLS DLKEAISA+AHKRNP F+KL
Sbjct: 241 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

BLAST of Sgr018948 vs. NCBI nr
Match: XP_004152122.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis sativus] >KGN53032.1 hypothetical protein Csa_014540 [Cucumis sativus])

HSP 1 Score: 455.7 bits (1171), Expect = 2.9e-124
Identity = 229/275 (83.27%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           MEDYK +KI R+NPNS VFHL LHRPSH+NALTT+LF+E  +A S LDQNPDV VIILSG
Sbjct: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFEK LS +RGRAGE+IRREIK MQ+SITAIE+CRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GC+GGG+DI+TACD+RYCT +A FSV+EV L ITADLGTLQRLP IVGYGKAAELALTGR
Sbjct: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
           NFSGLEAKELGLVSRTF S+  L+DGV KIAEEIAS SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLSDDLKEAISAQA KR P F+KL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQASKRKPKFSKL 275

BLAST of Sgr018948 vs. NCBI nr
Match: XP_008454026.1 (PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo] >TYK17024.1 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 450.7 bits (1158), Expect = 9.2e-123
Identity = 227/275 (82.55%), Postives = 251/275 (91.27%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           ME Y+ +KI R++PNS VFHL LHRPSH+NALTT+LF+E  +A S LDQNPDV VIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFEK LS +RGRAGE+IRREIK MQ+SITAIE+CRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GCIGGG+DI+TACD+RYCT +A FSV+EV L ITADLGTLQRLP IVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTF SM  L+DGV KIAEEIAS+SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLSDDLKEAISAQA+KR P F+KL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of Sgr018948 vs. ExPASy Swiss-Prot
Match: Q9FHR8 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=DCI1 PE=1 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 3.4e-104
Identity = 193/276 (69.93%), Postives = 230/276 (83.33%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           ME YK ++I+R+N +S+VFHL ++RPSH NAL+ + F EF +ALSSLDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSG-DRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIH 120
           +GK+FC+GIDL S SS   +  SG DRGR+ E++RR+IK+MQ +ITAIEQCRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTG 180
           G CIGGGVD+ITACDIRYC+EDAFFS+KEV+L I ADLGTLQRLP+IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RNFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQ 240
           R FSG EAK+LGLVS+ F S   L++GVT IAE I  KSPLAV GTKAVLL+SR+++VEQ
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQ 242

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           GLDYVATWNS  L+SDDL EA+SAQ  KR P FAKL
Sbjct: 243 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

BLAST of Sgr018948 vs. ExPASy Swiss-Prot
Match: Q62651 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ech1 PE=1 SV=2)

HSP 1 Score: 223.0 bits (567), Expect = 4.1e-57
Identity = 116/279 (41.58%), Postives = 171/279 (61.29%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           + D+ +  I   +    V H+ L+RP   NA+    + E       + ++ D R +++SG
Sbjct: 49  VSDHNYESIQVTSAQKHVLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSG 108

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           +GK F +GIDL   +S+  +    D  R    +R  I   Q + T IE+C KPVIA+IHG
Sbjct: 109 AGKMFTSGIDLMDMASDILQPPGDDVARIAWYLRDLISRYQKTFTVIEKCPKPVIAAIHG 168

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAA-ELALTG 180
           GCIGGGVD+I+ACDIRYCT+DAFF VKEV++G+ AD+GTLQRLP ++G      EL  T 
Sbjct: 169 GCIGGGVDLISACDIRYCTQDAFFQVKEVDVGLAADVGTLQRLPKVIGNRSLVNELTFTA 228

Query: 181 RNFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQ 240
           R     EA + GLVSR F   +++ +    +A +I+SKSP+AV G+K  L+ SRD +V++
Sbjct: 229 RKMMADEALDSGLVSRVFPDKDVMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDE 288

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQAHKRNP---AFAKL 276
            LDY+ATWN   L + D+ +++ A   K++     F+KL
Sbjct: 289 SLDYMATWNMSMLQTQDIIKSVQAAMEKKDSKSITFSKL 327

BLAST of Sgr018948 vs. ExPASy Swiss-Prot
Match: O35459 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus OX=10090 GN=Ech1 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.4e-57
Identity = 114/279 (40.86%), Postives = 169/279 (60.57%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           + D+ +  I   +    V H+ L+RP   NA+    + E       + ++ D R +++SG
Sbjct: 49  VSDHNYESIQVTSAQKHVLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSG 108

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           +GK F +GIDL   +S   +    D  R    +R  I   Q + T IE+C KPVIA+IHG
Sbjct: 109 AGKMFTSGIDLMDMASELMQPSGDDAARIAWYLRDLISKYQKTFTVIEKCPKPVIAAIHG 168

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVG-YGKAAELALTG 180
           GCIGGGVD+++ACDIRYCT+DAFF +KEV++G+ AD+GTLQRLP ++G      EL  + 
Sbjct: 169 GCIGGGVDLVSACDIRYCTQDAFFQIKEVDMGLAADVGTLQRLPKVIGNQSLVNELTFSA 228

Query: 181 RNFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQ 240
           R     EA + GLVSR F   + + +    +A +I+SKSP+AV G+K  L+ SRD +V++
Sbjct: 229 RKMMADEALDSGLVSRVFQDKDAMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDE 288

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQAHKRNP---AFAKL 276
            LDY+ATWN   L + D+ +++ A   KR+     F+KL
Sbjct: 289 SLDYMATWNMSMLQTQDIIKSVQAAMEKRDTKSITFSKL 327

BLAST of Sgr018948 vs. ExPASy Swiss-Prot
Match: Q5RFG0 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii OX=9601 GN=ECH1 PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 9.2e-57
Identity = 116/277 (41.88%), Postives = 168/277 (60.65%), Query Frame = 0

Query: 3   DYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSGSG 62
           D+ +  +   +    V H+ L+RP+  NA+    + E     + + ++ D R +++SG+G
Sbjct: 52  DHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAG 111

Query: 63  KNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHGGC 122
           K F AG+DL   +S+  +    D  R    +R  I   Q++   IE+C KPVIA++HGGC
Sbjct: 112 KMFTAGVDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIEKCPKPVIAAVHGGC 171

Query: 123 IGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVG-YGKAAELALTGRN 182
           IGGGVD++TACDIRYC +DAFF VKEV++G+ AD+GTLQRLP ++G      ELA T R 
Sbjct: 172 IGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARK 231

Query: 183 FSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQGL 242
               EA   GLVSR F   E++ D    +A EI+SKSP+AV  TK  LL SRD +V + L
Sbjct: 232 MMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESL 291

Query: 243 DYVATWNSGTLLSDDLKEAISAQAHK---RNPAFAKL 276
           +YVA+WN   L + DL +++ A       ++  F+KL
Sbjct: 292 NYVASWNMSMLQTQDLMKSVQAATENKELKSVTFSKL 328

BLAST of Sgr018948 vs. ExPASy Swiss-Prot
Match: Q13011 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens OX=9606 GN=ECH1 PE=1 SV=2)

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-56
Identity = 114/267 (42.70%), Postives = 163/267 (61.05%), Query Frame = 0

Query: 3   DYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSGSG 62
           D+ +  +   +    V H+ L+RP+  NA+    + E     + + ++ D R +++SG+G
Sbjct: 52  DHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAG 111

Query: 63  KNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHGGC 122
           K F AGIDL   +S+  +    D  R    +R  I   Q++   IE+C KPVIA++HGGC
Sbjct: 112 KMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGC 171

Query: 123 IGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVG-YGKAAELALTGRN 182
           IGGGVD++TACDIRYC +DAFF VKEV++G+ AD+GTLQRLP ++G      ELA T R 
Sbjct: 172 IGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARK 231

Query: 183 FSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQGL 242
               EA   GLVSR F   E++ D    +A EI+SKSP+AV  TK  LL SRD +V + L
Sbjct: 232 MMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESL 291

Query: 243 DYVATWNSGTLLSDDLKEAISAQAHKR 269
           +YVA+WN   L + DL +++ A    +
Sbjct: 292 NYVASWNMSMLQTQDLVKSVQATTENK 318

BLAST of Sgr018948 vs. ExPASy TrEMBL
Match: A0A6J1IPZ5 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111479454 PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.3e-125
Identity = 232/275 (84.36%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           MEDYK +KI R+NPNS VFHL LHRPSH+N LTTELF E  RALSSLDQNPDVRVIILSG
Sbjct: 1   MEDYKSIKIERQNPNSGVFHLRLHRPSHYNTLTTELFGELRRALSSLDQNPDVRVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFE LLS +RGRAGE++RRE+KAMQDSITAIE+CRKPVIA IHG
Sbjct: 61  SGKHFCAGIDLKSTASNFENLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GCIGGGVD+ TACD+RYCT +A FSVKEVNL ITADLGTLQRLP IVG+GKA ELALTGR
Sbjct: 121 GCIGGGVDMATACDLRYCTAEAVFSVKEVNLAITADLGTLQRLPGIVGFGKAVELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
            FSGLEAKELGLVS TF S   LE+GV KIAEEIASKSPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 EFSGLEAKELGLVSATFASKMELENGVIKIAEEIASKSPLAVVGTKAVLLKSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLS DLKEAISA+AHKRNPAF+KL
Sbjct: 241 LDYVATWNSGTLLSADLKEAISARAHKRNPAFSKL 275

BLAST of Sgr018948 vs. ExPASy TrEMBL
Match: A0A6J1GW60 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111458038 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.8e-125
Identity = 230/275 (83.64%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           MEDYK +KI R+NPNS VFHL LHRPSH+NALTTELF+E  RALSSLDQNPDVRVIILSG
Sbjct: 1   MEDYKSIKIERQNPNSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFE LLS +RGRAGE++RRE+KAMQDSITAIE+CRKPVIA IHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEDLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GCIGGGVDI TACD+RYCT +A FSVKE+NL ITADLGTLQRLP IVG+GKA ELALTGR
Sbjct: 121 GCIGGGVDIATACDLRYCTAEAVFSVKEINLAITADLGTLQRLPGIVGFGKAVELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
            FSG EAKELGLVS TF S   L++GV KIAEEIASKSPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 EFSGFEAKELGLVSTTFASKMELDNGVIKIAEEIASKSPLAVVGTKAVLLKSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLS DLKEAISA+AHKRNP F+KL
Sbjct: 241 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

BLAST of Sgr018948 vs. ExPASy TrEMBL
Match: A0A0A0KXB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011790 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.4e-124
Identity = 229/275 (83.27%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           MEDYK +KI R+NPNS VFHL LHRPSH+NALTT+LF+E  +A S LDQNPDV VIILSG
Sbjct: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFEK LS +RGRAGE+IRREIK MQ+SITAIE+CRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GC+GGG+DI+TACD+RYCT +A FSV+EV L ITADLGTLQRLP IVGYGKAAELALTGR
Sbjct: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
           NFSGLEAKELGLVSRTF S+  L+DGV KIAEEIAS SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLSDDLKEAISAQA KR P F+KL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQASKRKPKFSKL 275

BLAST of Sgr018948 vs. ExPASy TrEMBL
Match: A0A5D3D1B6 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001700 PE=3 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.5e-123
Identity = 227/275 (82.55%), Postives = 251/275 (91.27%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           ME Y+ +KI R++PNS VFHL LHRPSH+NALTT+LF+E  +A S LDQNPDV VIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFEK LS +RGRAGE+IRREIK MQ+SITAIE+CRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GCIGGG+DI+TACD+RYCT +A FSV+EV L ITADLGTLQRLP IVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTF SM  L+DGV KIAEEIAS+SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLSDDLKEAISAQA+KR P F+KL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of Sgr018948 vs. ExPASy TrEMBL
Match: A0A1S3BXS5 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103494575 PE=3 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.5e-123
Identity = 227/275 (82.55%), Postives = 251/275 (91.27%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           ME Y+ +KI R++PNS VFHL LHRPSH+NALTT+LF+E  +A S LDQNPDV VIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHG 120
           SGK+FCAGIDLKST+SNFEK LS +RGRAGE+IRREIK MQ+SITAIE+CRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGR 180
           GCIGGG+DI+TACD+RYCT +A FSV+EV L ITADLGTLQRLP IVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTF SM  L+DGV KIAEEIAS+SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           LDYVATWNSGTLLSDDLKEAISAQA+KR P F+KL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of Sgr018948 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 379.4 bits (973), Expect = 2.4e-105
Identity = 193/276 (69.93%), Postives = 230/276 (83.33%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           ME YK ++I+R+N +S+VFHL ++RPSH NAL+ + F EF +ALSSLDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSG-DRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIH 120
           +GK+FC+GIDL S SS   +  SG DRGR+ E++RR+IK+MQ +ITAIEQCRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTG 180
           G CIGGGVD+ITACDIRYC+EDAFFS+KEV+L I ADLGTLQRLP+IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RNFSGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQ 240
           R FSG EAK+LGLVS+ F S   L++GVT IAE I  KSPLAV GTKAVLL+SR+++VEQ
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQ 242

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQAHKRNPAFAKL 276
           GLDYVATWNS  L+SDDL EA+SAQ  KR P FAKL
Sbjct: 243 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

BLAST of Sgr018948 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 291.2 bits (744), Expect = 8.7e-79
Identity = 147/214 (68.69%), Postives = 179/214 (83.64%), Query Frame = 0

Query: 1   MEDYKFVKIVRRNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSG 60
           ME YK ++I+R+N +S+VFHL ++RPSH NAL+ + F EF +ALSSLDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKNFCAGIDLKSTSSNFEKLLSG-DRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIH 120
           +GK+FC+GIDL S SS   +  SG DRGR+ E++RR+IK+MQ +ITAIEQCRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCIGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTG 180
           G CIGGGVD+ITACDIRYC+EDAFFS+KEV+L I ADLGTLQRLP+IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RNFSGLEAKELGLVSRTFTSMELLEDGVTKIAEE 214
           R FSG EAK+LGLVS+ F S   L++GVT IAE+
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAED 216

BLAST of Sgr018948 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 126.3 bits (316), Expect = 3.7e-29
Identity = 82/270 (30.37%), Postives = 131/270 (48.52%), Query Frame = 0

Query: 5   KFVKIVR-RNPNSAVFHLHLHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSGSGK 64
           +FVK+ R    +S +  ++L RP   NA+  E+      A  S+ Q+   RV+++     
Sbjct: 41  EFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIRSLVP 100

Query: 65  N-FCAGIDLKSTSSNFEKLLSGDRGRAGEKIRREIKAMQDSITAIEQCRKPVIASIHGGC 124
             FCAG DLK             R  +  ++   + +++   + IE    P IA+I G  
Sbjct: 101 GVFCAGADLKER-----------RTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAA 160

Query: 125 IGGGVDIITACDIRYCTEDAFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGRNF 184
           +GGG+++  ACD+R C E+A F + E  L I    G  QRL  +VG   + EL  TGR  
Sbjct: 161 LGGGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKI 220

Query: 185 SGLEAKELGLVSRTFTSMELLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQGLD 244
             +EA   GLV+   T+ E  E  + ++A++I  K PLA+   K  + +  + N+  GL+
Sbjct: 221 DAIEAANKGLVNICVTAGEAHEKAI-EMAQQINEKGPLAIKMAKKAIDEGIETNMASGLE 280

Query: 245 YVATWNSGTLLSDDLKEAISAQAHKRNPAF 273
                    L + D  E ++A A KR P +
Sbjct: 281 VEEMCYQKLLNTQDRLEGLAAFAEKRKPLY 298

BLAST of Sgr018948 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 98.6 bits (244), Expect = 8.3e-21
Identity = 64/220 (29.09%), Postives = 107/220 (48.64%), Query Frame = 0

Query: 23  LHRPSHHNALTTELFSEFGRALSSLDQNPDVRVIILSGSGKNFCAGIDLKSTSSNFEKLL 82
           ++RP   N+LT  +  +  +A   +D +  V+V+I +GSG++FC+G+DL +  S F    
Sbjct: 24  INRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRSFCSGVDLTAAESVF---- 83

Query: 83  SGDRGRAGEKIRREIKAMQ-DSITAIEQCRKPVIASIHGGCIGGGVDIITACDIRYCTED 142
                      + ++K  + D +  +E+ RKP+I +I+G  I  G ++  ACDI   +  
Sbjct: 84  -----------KGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALACDILVASRG 143

Query: 143 AFFSVKEVNLGITADLGTLQRLPAIVGYGKAAELALTGRNFSGLEAKELGLVSRTFTSME 202
           A F       GI    G  Q+L  I+G  KA E++LT    +   A +LG V+      E
Sbjct: 144 AKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFVNHVVEEGE 203

Query: 203 LLEDGVTKIAEEIASKSPLAVVGTKAVLLKSRDLNVEQGL 242
            L+    +IAE I       V+  K+V+     L++   L
Sbjct: 204 ALKK-AREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHAL 227

BLAST of Sgr018948 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 80.9 bits (198), Expect = 1.8e-15
Identity = 50/166 (30.12%), Postives = 82/166 (49.40%), Query Frame = 0

Query: 30  NALTTELFSEFGRALSSLDQNPDVRVIILSGSGKNFCAGIDLKSTSSNFEKL-LSGDRGR 89
           N+L + + S         +Q  DV+ I+L G+   F  G D+    + F+++  +GD   
Sbjct: 27  NSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGRFSGGFDI----NVFQQVHKTGDLSL 86

Query: 90  AGE-KIRREIKAMQDSITAIEQCRKPVIASIHGGCIGGGVDIITACDIRYCTEDAFFSVK 149
             E  +      M+DS       RKPV+A++ G  +GGG+++  AC  R     A   + 
Sbjct: 87  MPEVSVELVCNLMEDS-------RKPVVAAVEGLALGGGLELAMACHARVAAPKAQLGLP 146

Query: 150 EVNLGITADLGTLQRLPAIVGYGKAAELALTGRNFSGLEAKELGLV 194
           E+ LG+    G  QRLP +VG  KA ++ L  ++ S  E  +LGL+
Sbjct: 147 ELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISSEEGHKLGLI 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022979902.12.6e-12584.36delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita maxima][more]
XP_023526792.17.6e-12584.00delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita pepo subsp. ... [more]
XP_022956301.19.9e-12583.64delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita moschata] >K... [more]
XP_004152122.12.9e-12483.27delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis sativus] >KGN5... [more]
XP_008454026.19.2e-12382.55PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis mel... [more]
Match NameE-valueIdentityDescription
Q9FHR83.4e-10469.93Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana... [more]
Q626514.1e-5741.58Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus ... [more]
O354595.4e-5740.86Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus OX=10... [more]
Q5RFG09.2e-5741.88Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii OX=96... [more]
Q130111.2e-5642.70Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens OX=96... [more]
Match NameE-valueIdentityDescription
A0A6J1IPZ51.3e-12584.36delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita maxima OX=... [more]
A0A6J1GW604.8e-12583.64delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita moschata O... [more]
A0A0A0KXB81.4e-12483.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011790 PE=3 SV=1[more]
A0A5D3D1B64.5e-12382.55Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. ma... [more]
A0A1S3BXS54.5e-12382.55delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
AT5G43280.12.4e-10569.93delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
AT5G43280.28.7e-7968.69delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
AT4G16800.13.7e-2930.37ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT4G16210.18.3e-2129.09enoyl-CoA hydratase/isomerase A [more]
AT4G29010.11.8e-1530.12Enoyl-CoA hydratase/isomerase family [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.226.10coord: 1..217
e-value: 3.4E-65
score: 221.5
NoneNo IPR availableCDDcd06558crotonase-likecoord: 17..214
e-value: 1.60924E-69
score: 211.265
IPR014748Enoyl-CoA hydratase, C-terminalGENE3D1.10.12.10coord: 218..275
e-value: 6.0E-24
score: 86.0
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 15..273
e-value: 5.0E-51
score: 173.4
IPR045002Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase Ech1-likePANTHERPTHR43149ENOYL-COA HYDRATASEcoord: 2..275
IPR018376Enoyl-CoA hydratase/isomerase, conserved sitePROSITEPS00166ENOYL_COA_HYDRATASEcoord: 115..135
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 10..273

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr018948.1Sgr018948.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044281 small molecule metabolic process
cellular_component GO:0005777 peroxisome
molecular_function GO:0051750 delta3,5-delta2,4-dienoyl-CoA isomerase activity
molecular_function GO:0003824 catalytic activity