Sgr018807 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr018807
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	4-coumarate--CoA ligase
Location	tig00153210: 807451 .. 808585 (+)
RNA-Seq Expression	Sgr018807
Synteny	Sgr018807

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGGTGAAGAATCGTGCACTGCTCCGGCAGCCGTGGAATATCAACGGCAGAATGCTCCGTCGCCGACGACTGCTACCGGCGGTTATGATGTGAATACTGCGGTTTATCATTCAGTTGTGGAGCTTGATGAAGCTGATCGTAAGATCTCCAGCAGGCCAGACCTTGACACGGCCACTTTCGTCTTATCGCAGTTTCCCAAGGCCGAATCTCGAGTTGCTCTCATTGACTCGGTTACGAGCTCGCGAGTTACTTATGGACAACTCAGTGCGTCGATTCGTTCCCTCGCTTGCGGACTTTACCACGCACTCGGAGTTCGAAAAGGCGATGTCGTGTTCGTTTTATCGGCGAATTCAGTTCTGTATCCGTCATCTGTTTGGCTGTGTTATCGGTTGGGCAGTAATTACAACGGCCAATCCAATCAATACTGCGTCGGAAATCGCCAAGCAAGTGCGTGACTCAGGTGCGAAACTGGCTGTATCGGCGCCGGAGGAGCTTCACAAATTAGTTCCTACAGGAGTTCCGACGATTCTGACCACTCGTCCCTCCTATGGCGATGATTTGTCGGTGGAAGAGTTGATCGAATGCTGTATCGACTCGTTTGAGTCATTGCCGAACACGGAACTAACTCAGTCAGATACGGCGGCGATCTTGTACTCGTCGGGAACGACCGGGACGAGTAAAGGAGTAATTCTGACGCATGCAAATCTCATCTCCGTTATGGAACTTCTAATCTGGTACGTGGACGCGAGCTGCTCTCAAAACGACGTGTTTTTGTGCTTCATTCCAATGTTTCACATCTACGGCCTCGTCTTCTTCGCGCTCGGACTCTTCTGCAGAGGAACCACCACGGTTTTGATGCAGAGATTCAATTTCCAAGCCATGATCGACGCAATAGAAACATTCGAAGTCAACAACGTACCGGCAGTACCTCCGGTGATCCTAGGGCTAGTGAAATCGGGCGATGGTTCGGACTTCTCATCGCTGAGACGCATCGGATCCGGAGCTGCACCCTTGGAAAAGACGTGGAAGAAGCTTTCGAAAGAAATTTCCATGGGTGGAGCTTCGGCCAGGATATGGCTTAACCGAGAGCACCGGAGCAGCGACTTGCTTTCTTACAGACAAAGACGCTAG

mRNA sequence

ATGTCCGGTGAAGAATCGTGCACTGCTCCGGCAGCCGTGGAATATCAACGGCAGAATGCTCCGTCGCCGACGACTGCTACCGGCGGTTATGATGTGAATACTGCGGTTTATCATTCAGTTGTGGAGCTTGATGAAGCTGATCGTAAGATCTCCAGCAGGCCAGACCTTGACACGGCCACTTTCGTCTTATCGCAGTTTCCCAAGGCCGAATCTCGAGTTGCTCTCATTGACTCGGTTACGAGCTCGCGAGTTACTTATGGACAACTCATAATTACAACGGCCAATCCAATCAATACTGCGTCGGAAATCGCCAAGCAAGTGCGTGACTCAGGTGCGAAACTGGCTGTATCGGCGCCGGAGGAGCTTCACAAATTAGTTCCTACAGGAGTTCCGACGATTCTGACCACTCGTCCCTCCTATGGCGATGATTTGTCGGTGGAAGAGTTGATCGAATGCTGTATCGACTCGTTTGAGTCATTGCCGAACACGGAACTAACTCAGTCAGATACGGCGGCGATCTTGTACTCGTCGGGAACGACCGGGACGAGTAAAGGAGTAATTCTGACGCATGCAAATCTCATCTCCGTTATGGAACTTCTAATCTGGTACGTGGACGCGAGCTGCTCTCAAAACGACGTGTTTTTGTGCTTCATTCCAATGTTTCACATCTACGGCCTCGTCTTCTTCGCGCTCGGACTCTTCTGCAGAGGAACCACCACGGTTTTGATGCAGAGATTCAATTTCCAAGCCATGATCGACGCAATAGAAACATTCGAAGTCAACAACGTACCGGCAGTACCTCCGGTGATCCTAGGGCTAGTGAAATCGGGCGATGGTTCGGACTTCTCATCGCTGAGACGCATCGGATCCGGAGCTGCACCCTTGGAAAAGACGTGGAAGAAGCTTTCGAAAGAAATTTCCATGGGTGGAGCTTCGGCCAGGATATGGCTTAACCGAGAGCACCGGAGCAGCGACTTGCTTTCTTACAGACAAAGACGCTAG

Coding sequence (CDS)

ATGTCCGGTGAAGAATCGTGCACTGCTCCGGCAGCCGTGGAATATCAACGGCAGAATGCTCCGTCGCCGACGACTGCTACCGGCGGTTATGATGTGAATACTGCGGTTTATCATTCAGTTGTGGAGCTTGATGAAGCTGATCGTAAGATCTCCAGCAGGCCAGACCTTGACACGGCCACTTTCGTCTTATCGCAGTTTCCCAAGGCCGAATCTCGAGTTGCTCTCATTGACTCGGTTACGAGCTCGCGAGTTACTTATGGACAACTCATAATTACAACGGCCAATCCAATCAATACTGCGTCGGAAATCGCCAAGCAAGTGCGTGACTCAGGTGCGAAACTGGCTGTATCGGCGCCGGAGGAGCTTCACAAATTAGTTCCTACAGGAGTTCCGACGATTCTGACCACTCGTCCCTCCTATGGCGATGATTTGTCGGTGGAAGAGTTGATCGAATGCTGTATCGACTCGTTTGAGTCATTGCCGAACACGGAACTAACTCAGTCAGATACGGCGGCGATCTTGTACTCGTCGGGAACGACCGGGACGAGTAAAGGAGTAATTCTGACGCATGCAAATCTCATCTCCGTTATGGAACTTCTAATCTGGTACGTGGACGCGAGCTGCTCTCAAAACGACGTGTTTTTGTGCTTCATTCCAATGTTTCACATCTACGGCCTCGTCTTCTTCGCGCTCGGACTCTTCTGCAGAGGAACCACCACGGTTTTGATGCAGAGATTCAATTTCCAAGCCATGATCGACGCAATAGAAACATTCGAAGTCAACAACGTACCGGCAGTACCTCCGGTGATCCTAGGGCTAGTGAAATCGGGCGATGGTTCGGACTTCTCATCGCTGAGACGCATCGGATCCGGAGCTGCACCCTTGGAAAAGACGTGGAAGAAGCTTTCGAAAGAAATTTCCATGGGTGGAGCTTCGGCCAGGATATGGCTTAACCGAGAGCACCGGAGCAGCGACTTGCTTTCTTACAGACAAAGACGCTAG

Protein sequence

MSGEESCTAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTATFVLSQFPKAESRVALIDSVTSSRVTYGQLIITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILTTRPSYGDDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLISVMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAIETFEVNNVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKTWKKLSKEISMGGASARIWLNREHRSSDLLSYRQRR

Homology

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BLAST of Sgr018807 vs. NCBI nr
Match: XP_022138968.1 (4-coumarate--CoA ligase-like 5 [Momordica charantia])

HSP 1 Score: 462.6 bits (1189), Expect = 2.8e-126
Identity = 256/343 (74.64%), Postives = 272/343 (79.30%), Query Frame = 0


Query: 1   MSGEESCTAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTAT 60
           MSGEES TAPAA EY RQNAP P  + GGYDVN AVYHS+V+ DEA RKISSRPDLDTAT
Sbjct: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNIAVYHSLVKFDEA-RKISSRPDLDTAT 60

Query: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------- 120
           FVLSQFPKAESRVALIDSVTSSRVTYGQL                               
Sbjct: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSI 120

Query: 121 --------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILT 180
                         +ITTANP+NTASEIAKQVRDSGAKLAVSAPEE+HKL PTG+PTILT
Sbjct: 121 LYPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILT 180

Query: 181 TRPSYGDD-LSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLI 240
           TRPS GDD LSVEELIE C DS ESLPNTE+TQSDTAAILYSSGTTGTSKGV+LTH+NLI
Sbjct: 181 TRPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLI 240

Query: 241 SVMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDA 298
           SV+ELL  YVDAS S+NDVFLCFIPMFHIYGLVFF LGLFCRG T +LMQRFNFQAMIDA
Sbjct: 241 SVIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDA 300

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BLAST of Sgr018807 vs. NCBI nr
Match: XP_023523152.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 449.9 bits (1156), Expect = 1.9e-122
Identity = 244/350 (69.71%), Postives = 273/350 (78.00%), Query Frame = 0


Query: 1   MSGEESCTAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTAT 60
           MS EESCTAPAAV+++  N+P      GGYDVNTAVYHS+V+LDEA  KIS+ PDLDTAT
Sbjct: 1   MSREESCTAPAAVDFRPMNSP------GGYDVNTAVYHSLVQLDEAP-KISTSPDLDTAT 60

Query: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------- 120
           FVLSQFP AESRVALIDSVTSSRVTYG+L                               
Sbjct: 61  FVLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSV 120

Query: 121 --------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILT 180
                         +ITTANP+NTASEI KQV DS AKLA+SAPEELHKL+PTG+PTILT
Sbjct: 121 LYPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILT 180

Query: 181 TRPSYGDDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLIS 240
           TRPSYGD LSVEELIE CI+SFE +P T++TQSDTAAILYSSGTTGTSKGV+LTH+NLIS
Sbjct: 181 TRPSYGDVLSVEELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAI 300
           V+ELL WYVDAS SQ+DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 ETFEVNNVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKTWKKLSKE 306
           ET++VNN+PAVPPVILGLVKS  GSD SSLRRIGSGAAPL K  ++  +E
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSSGSDLSSLRRIGSGAAPLGKDVEEAFRE 343

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BLAST of Sgr018807 vs. NCBI nr
Match: KAG7031989.1 (4-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 446.0 bits (1146), Expect = 2.8e-121
Identity = 243/350 (69.43%), Postives = 271/350 (77.43%), Query Frame = 0


Query: 1   MSGEESCTAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTAT 60
           MS EESCTAPAAVE++  N+P      GGYDVNTAVYHS+V+LDEA  KIS+ PDLDTAT
Sbjct: 1   MSREESCTAPAAVEFRPMNSP------GGYDVNTAVYHSLVQLDEAS-KISTSPDLDTAT 60

Query: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------- 120
           FVLSQFP AESRVALIDSVTSSRVTYG+L                               
Sbjct: 61  FVLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSV 120

Query: 121 --------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILT 180
                         +ITTANP+NTASEI KQV DS AKLA+SAPEELHKL PTG+PTILT
Sbjct: 121 LYPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILT 180

Query: 181 TRPSYGDDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLIS 240
           TRPSYGD LSVEELIE CI++FE +P T++ QSDTAAILYSSGTTGTSKGV+LTH+NLIS
Sbjct: 181 TRPSYGDVLSVEELIESCIETFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAI 300
           V+ELL WYVDAS SQ+DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 ETFEVNNVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKTWKKLSKE 306
           ET++VNN+PAVPPVILGLVKS  GSD SSLRRIGSGAAPL K  ++  +E
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFRE 343

[Close]

BLAST of Sgr018807 vs. NCBI nr
Match: XP_022957594.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 444.5 bits (1142), Expect = 8.0e-121
Identity = 244/350 (69.71%), Postives = 270/350 (77.14%), Query Frame = 0


Query: 1   MSGEESCTAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTAT 60
           MS EESCTAPAAVE++  N+P      GGYDVNTAVYHS+V+ DEA  KIS+ PDLDTAT
Sbjct: 1   MSREESCTAPAAVEFRPINSP------GGYDVNTAVYHSLVQFDEAP-KISTSPDLDTAT 60

Query: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------- 120
           FVLSQFP AESRVALIDSVTSSRVTYG+L                               
Sbjct: 61  FVLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSV 120

Query: 121 --------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILT 180
                         +ITTANP+NTASEI KQV DS AKLA+SAPEELHKL PTGVPTILT
Sbjct: 121 LYPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILT 180

Query: 181 TRPSYGDDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLIS 240
           TRPSYGD LSVEELIE CI+SFE +P T++ QSDTAAILYSSGTTGTSKGV+LTH+NLIS
Sbjct: 181 TRPSYGDVLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAI 300
           V+ELL WYVDAS SQ+DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 ETFEVNNVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKTWKKLSKE 306
           ET++VNN+PAVPPVILGLVKS  GSD SSLRRIGSGAAPL K  ++  +E
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFRE 343

[Close]

BLAST of Sgr018807 vs. NCBI nr
Match: XP_022994494.1 (4-coumarate--CoA ligase-like 5 [Cucurbita maxima])

HSP 1 Score: 442.6 bits (1137), Expect = 3.0e-120
Identity = 242/350 (69.14%), Postives = 269/350 (76.86%), Query Frame = 0


Query: 1   MSGEESCTAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTAT 60
           MS EESCTAPAAVE++  N+P      GGYDVNTAVYHS V+LDEA  KIS+ PDLDTAT
Sbjct: 1   MSREESCTAPAAVEFRPMNSP------GGYDVNTAVYHSFVQLDEAP-KISTSPDLDTAT 60

Query: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------- 120
           FVLSQFP AESRVALIDSVTSSRVTYG+L                               
Sbjct: 61  FVLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSV 120

Query: 121 --------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILT 180
                         +ITTANP+NTASEI KQV DS AKLA+SAPEELHKL PTG+PTILT
Sbjct: 121 LYPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILT 180

Query: 181 TRPSYGDDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLIS 240
           T+PSYGD LSVEELIE CI+SFE +P T++ QSD AAILYSSGTTGTSKGV+LTH+NLIS
Sbjct: 181 TQPSYGDVLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAI 300
           V+ELL WYVDAS SQ+DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 ETFEVNNVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKTWKKLSKE 306
           ET++VNN+PAVPPVILGLVKS  GSD SSLRRIGSGAAPL K  ++  +E
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFRE 343

[Close]

BLAST of Sgr018807 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.4e-35
Identity = 90/218 (41.28%), Postives = 127/218 (58.26%), Query Frame = 0


Query: 90  IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTIL--------TTRPSYG 149
           I TT+NP+ T SE++KQV+DS  KL V+ PE   K+    +PTIL        +   S  
Sbjct: 105 IATTSNPLYTVSELSKQVKDSNPKLIVTVPELFEKVKGFNLPTILIGPNSEDSSPLKSRA 164

Query: 150 DDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLISVMELLI 209
             L+  +L+          P  +  QSDTAA+LYSSGTTG SKGV+L+H N I+   ++ 
Sbjct: 165 KVLTFHDLVTLS-GPVSDFPMVDFKQSDTAALLYSSGTTGMSKGVVLSHKNFIASSLMVT 224

Query: 210 WYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAIETFEVN 269
              D +   ++VFLCF+PMFH++GL         RG T + M RF+ + ++  +E ++V 
Sbjct: 225 MEQDQAGEMHNVFLCFLPMFHVFGLAIITYAQLQRGNTVISMARFDLEKILKDVEKYKVT 284

Query: 270 NVPAVPPVILGLVKSG--DGSDFSSLRRIGSGAAPLEK 298
           ++  VPPVIL L K+      D SSL+ IGSGAAPL K
Sbjct: 285 HLWVVPPVILALTKNSIVKKYDLSSLKHIGSGAAPLGK 321

[Close]

BLAST of Sgr018807 vs. ExPASy Swiss-Prot
Match: Q84P24 (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=2)

HSP 1 Score: 145.2 bits (365), Expect = 1.3e-33
Identity = 84/216 (38.89%), Postives = 127/216 (58.80%), Query Frame = 0


Query: 90  IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILTTRPSYGDDLSVE-- 149
           I+TT NP ++  EI KQV +    LA ++ E + KL   GV  I  +     D + +E  
Sbjct: 124 IVTTMNPSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENP 183

Query: 150 ELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLISVMELLIWYVDAS 209
           +      +SF  +P   + Q D AAI+YSSGTTG SKGV+LTH NLI+ MEL + +  + 
Sbjct: 184 KFYSIMKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQ 243

Query: 210 C---SQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAIETFEVNNVP 269
                 ++V+L  +P+ HIYGL  F +GL   G+T V+M+RF+   +++ IE F++ + P
Sbjct: 244 YEYPGSSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFP 303

Query: 270 AVPPVILGLVKSGD---GSDFSSLRRIGSGAAPLEK 298
            VPP+++ L K      G  F SL+++ SGAAPL +
Sbjct: 304 VVPPMLMALTKKAKGVCGEVFKSLKQVSSGAAPLSR 339

[Close]

BLAST of Sgr018807 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.6e-33
Identity = 89/218 (40.83%), Postives = 122/218 (55.96%), Query Frame = 0


Query: 90  IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTIL-----TTRPSYGDD- 149
           + TTANP+ T +E++KQ++DS  K+ +S  +   K+    +P +L     T     G + 
Sbjct: 105 VFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNS 164

Query: 150 --LSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLISVMELLI 209
             LS + ++E   +     P  E+ QSDTAA+LYSSGTTGTSKGV LTH N I+   ++ 
Sbjct: 165 KILSFDNVMELS-EPVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVT 224

Query: 210 WYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAIETFEVN 269
              D     + VFLCF+PMFH++GL         RG   V M RF  + ++  IE F V 
Sbjct: 225 MDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVT 284

Query: 270 NVPAVPPVILGLVKSG--DGSDFSSLRRIGSGAAPLEK 298
           ++  VPPV L L K       D SSL+ IGSGAAPL K
Sbjct: 285 HLWVVPPVFLALSKQSIVKKFDLSSLKYIGSGAAPLGK 321

[Close]

BLAST of Sgr018807 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.5e-32
Identity = 85/224 (37.95%), Postives = 129/224 (57.59%), Query Frame = 0


Query: 90  IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILTTRPSYGDDLSVEEL 149
           IITT NP+NT  EIAKQ+ DS   LA + P+ + K+  + +P ++        D  V+  
Sbjct: 113 IITTTNPLNTPREIAKQITDSKPVLAFTIPQLVSKIAGSNLPIVII-------DDEVKSS 172

Query: 150 IECCIDSFESL---------PNT---ELTQSDTAAILYSSGTTGTSKGVILTHANLISVM 209
           +E  ++   SL         PN     + Q DTA +LYSSGTTG SKGV+ +H NLI+++
Sbjct: 173 LEKTLNIVSSLGEMMRKEPSPNRIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMV 232

Query: 210 ELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAIET 269
           + ++        ++  F+C +PMFHIYGL  FA+GL   G+T V++ +F    M+ AIE 
Sbjct: 233 QTILSRFGTEDGEH-TFICTVPMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEK 292

Query: 270 FEVNNVPAVPPVILGLVKSGD----GSDFSSLRRIGSGAAPLEK 298
           +    +P VPP+++ L+K+ +      D SSL+ + SG APL K
Sbjct: 293 YRATYLPLVPPILMALLKNANHIRAKYDLSSLQSVLSGGAPLSK 328

[Close]

BLAST of Sgr018807 vs. ExPASy Swiss-Prot
Match: Q336M7 (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 138.3 bits (347), Expect = 1.6e-31
Identity = 78/217 (35.94%), Postives = 126/217 (58.06%), Query Frame = 0


Query: 90  IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILT--TRPSYGDDLSVE 149
           + TT NP +  +EIA ++RD+   L +++     KL P   P +L   T     DD   +
Sbjct: 116 VATTMNPSSAPAEIAARLRDTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFD 175

Query: 150 ELIECCIDSFESLP---NTELTQSDTAAILYSSGTTGTSKGVILTHANLISVMELLIWYV 209
                 +++    P      + Q D AA+LYSSGT+G SKGV++TH NLI+++EL + + 
Sbjct: 176 FFFHALLETDPETPVEMGVGVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFE 235

Query: 210 DASC---SQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAIETFEVN 269
            +     ++++V+L  +PMFH+YGL  FA+GL   G T V+M+RFN    + AI  ++V 
Sbjct: 236 ASQYTRPARDNVYLAALPMFHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVT 295

Query: 270 NVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKT 299
           ++P VPP++  L+++    +  SL ++ SGAAPL  T
Sbjct: 296 HLPLVPPIMSALLRANPPLELDSLLQVSSGAAPLNHT 332

[Close]

BLAST of Sgr018807 vs. ExPASy TrEMBL
Match: A0A6J1CEK1 (4-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.4e-126
Identity = 256/343 (74.64%), Postives = 272/343 (79.30%), Query Frame = 0


Query: 1   MSGEESCTAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTAT 60
           MSGEES TAPAA EY RQNAP P  + GGYDVN AVYHS+V+ DEA RKISSRPDLDTAT
Sbjct: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNIAVYHSLVKFDEA-RKISSRPDLDTAT 60

Query: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------- 120
           FVLSQFPKAESRVALIDSVTSSRVTYGQL                               
Sbjct: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSI 120

Query: 121 --------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILT 180
                         +ITTANP+NTASEIAKQVRDSGAKLAVSAPEE+HKL PTG+PTILT
Sbjct: 121 LYPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILT 180

Query: 181 TRPSYGDD-LSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLI 240
           TRPS GDD LSVEELIE C DS ESLPNTE+TQSDTAAILYSSGTTGTSKGV+LTH+NLI
Sbjct: 181 TRPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLI 240

Query: 241 SVMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDA 298
           SV+ELL  YVDAS S+NDVFLCFIPMFHIYGLVFF LGLFCRG T +LMQRFNFQAMIDA
Sbjct: 241 SVIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDA 300

[Close]

BLAST of Sgr018807 vs. ExPASy TrEMBL
Match: A0A6J1H2E6 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.9e-121
Identity = 244/350 (69.71%), Postives = 270/350 (77.14%), Query Frame = 0


Query: 1   MSGEESCTAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTAT 60
           MS EESCTAPAAVE++  N+P      GGYDVNTAVYHS+V+ DEA  KIS+ PDLDTAT
Sbjct: 1   MSREESCTAPAAVEFRPINSP------GGYDVNTAVYHSLVQFDEAP-KISTSPDLDTAT 60

Query: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------- 120
           FVLSQFP AESRVALIDSVTSSRVTYG+L                               
Sbjct: 61  FVLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSV 120

Query: 121 --------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILT 180
                         +ITTANP+NTASEI KQV DS AKLA+SAPEELHKL PTGVPTILT
Sbjct: 121 LYPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILT 180

Query: 181 TRPSYGDDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLIS 240
           TRPSYGD LSVEELIE CI+SFE +P T++ QSDTAAILYSSGTTGTSKGV+LTH+NLIS
Sbjct: 181 TRPSYGDVLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAI 300
           V+ELL WYVDAS SQ+DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 ETFEVNNVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKTWKKLSKE 306
           ET++VNN+PAVPPVILGLVKS  GSD SSLRRIGSGAAPL K  ++  +E
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFRE 343

[Close]

BLAST of Sgr018807 vs. ExPASy TrEMBL
Match: A0A6J1K303 (4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.5e-120
Identity = 242/350 (69.14%), Postives = 269/350 (76.86%), Query Frame = 0


Query: 1   MSGEESCTAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTAT 60
           MS EESCTAPAAVE++  N+P      GGYDVNTAVYHS V+LDEA  KIS+ PDLDTAT
Sbjct: 1   MSREESCTAPAAVEFRPMNSP------GGYDVNTAVYHSFVQLDEAP-KISTSPDLDTAT 60

Query: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------- 120
           FVLSQFP AESRVALIDSVTSSRVTYG+L                               
Sbjct: 61  FVLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSV 120

Query: 121 --------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILT 180
                         +ITTANP+NTASEI KQV DS AKLA+SAPEELHKL PTG+PTILT
Sbjct: 121 LYPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILT 180

Query: 181 TRPSYGDDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLIS 240
           T+PSYGD LSVEELIE CI+SFE +P T++ QSD AAILYSSGTTGTSKGV+LTH+NLIS
Sbjct: 181 TQPSYGDVLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAI 300
           V+ELL WYVDAS SQ+DVFLCF+PMFHIYGLVFF LGLFCRGTT VLMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 ETFEVNNVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKTWKKLSKE 306
           ET++VNN+PAVPPVILGLVKS  GSD SSLRRIGSGAAPL K  ++  +E
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFRE 343

[Close]

BLAST of Sgr018807 vs. ExPASy TrEMBL
Match: A0A5A7SZP5 (4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002300 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.6e-114
Identity = 236/351 (67.24%), Postives = 265/351 (75.50%), Query Frame = 0


Query: 1   MSGEESC-TAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTA 60
           MS EESC T PAA+E+Q +N+P      GGY+VNTAVYHS++  DEA   IS+R DLDTA
Sbjct: 1   MSDEESCSTGPAAMEFQPRNSP------GGYNVNTAVYHSLIHFDEAP-VISTRSDLDTA 60

Query: 61  TFVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------ 120
           T+VLSQFP AESRVALIDSVTS RVTYGQL                              
Sbjct: 61  TYVLSQFPTAESRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANC 120

Query: 121 ---------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTIL 180
                          +ITTANPINTASEI KQVRDSGAKLAVSAPEEL KL PTG+PTIL
Sbjct: 121 VLYPVICLAVLSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTIL 180

Query: 181 TTRPSYGDDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLI 240
           TTR S+ D LSVEELIE C +S E LP TE+TQSDTAAILYSSGTTGTSKGV+LTH+NLI
Sbjct: 181 TTRSSFSDALSVEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLI 240

Query: 241 SVMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDA 300
           SV+ELL W VD++ SQ+DVFLCFIPMFHIYGLVFF LGLFCRG TTVLMQRFNFQ+MIDA
Sbjct: 241 SVIELLTWCVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDA 300

Query: 301 IETFEVNNVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKTWKKLSKE 306
           IE +++NN+PAVPPVILGLVKSG GSDFSSLRR+GSGAAPL K  ++  +E
Sbjct: 301 IEKYKINNIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFRE 344

[Close]

BLAST of Sgr018807 vs. ExPASy TrEMBL
Match: A0A1S4DW24 (4-coumarate--CoA ligase-like 5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489425 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.6e-114
Identity = 236/351 (67.24%), Postives = 265/351 (75.50%), Query Frame = 0


Query: 1   MSGEESC-TAPAAVEYQRQNAPSPTTATGGYDVNTAVYHSVVELDEADRKISSRPDLDTA 60
           MS EESC T PAA+E+Q +N+P      GGY+VNTAVYHS++  DEA   IS+R DLDTA
Sbjct: 1   MSDEESCSTGPAAMEFQPRNSP------GGYNVNTAVYHSLIHFDEAP-VISTRSDLDTA 60

Query: 61  TFVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------------ 120
           T+VLSQFP AESRVALIDSVTS RVTYGQL                              
Sbjct: 61  TYVLSQFPTAESRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANC 120

Query: 121 ---------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTIL 180
                          +ITTANPINTASEI KQVRDSGAKLAVSAPEEL KL PTG+PTIL
Sbjct: 121 VLYPVICLAVLSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTIL 180

Query: 181 TTRPSYGDDLSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLI 240
           TTR S+ D LSVEELIE C +S E LP TE+TQSDTAAILYSSGTTGTSKGV+LTH+NLI
Sbjct: 181 TTRSSFSDALSVEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLI 240

Query: 241 SVMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDA 300
           SV+ELL W VD++ SQ+DVFLCFIPMFHIYGLVFF LGLFCRG TTVLMQRFNFQ+MIDA
Sbjct: 241 SVIELLTWCVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDA 300

Query: 301 IETFEVNNVPAVPPVILGLVKSGDGSDFSSLRRIGSGAAPLEKTWKKLSKE 306
           IE +++NN+PAVPPVILGLVKSG GSDFSSLRR+GSGAAPL K  ++  +E
Sbjct: 301 IEKYKINNIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFRE 344

[Close]

BLAST of Sgr018807 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 145.2 bits (365), Expect = 9.4e-35
Identity = 84/216 (38.89%), Postives = 127/216 (58.80%), Query Frame = 0


Query: 90  IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILTTRPSYGDDLSVE-- 149
           I+TT NP ++  EI KQV +    LA ++ E + KL   GV  I  +     D + +E  
Sbjct: 124 IVTTMNPSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENP 183

Query: 150 ELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLISVMELLIWYVDAS 209
           +      +SF  +P   + Q D AAI+YSSGTTG SKGV+LTH NLI+ MEL + +  + 
Sbjct: 184 KFYSIMKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQ 243

Query: 210 C---SQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAIETFEVNNVP 269
                 ++V+L  +P+ HIYGL  F +GL   G+T V+M+RF+   +++ IE F++ + P
Sbjct: 244 YEYPGSSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFP 303

Query: 270 AVPPVILGLVKSGD---GSDFSSLRRIGSGAAPLEK 298
            VPP+++ L K      G  F SL+++ SGAAPL +
Sbjct: 304 VVPPMLMALTKKAKGVCGEVFKSLKQVSSGAAPLSR 339

[Close]

BLAST of Sgr018807 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 142.5 bits (358), Expect = 6.1e-34
Identity = 89/218 (40.83%), Postives = 122/218 (55.96%), Query Frame = 0


Query: 90  IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTIL-----TTRPSYGDD- 149
           + TTANP+ T +E++KQ++DS  K+ +S  +   K+    +P +L     T     G + 
Sbjct: 105 VFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNS 164

Query: 150 --LSVEELIECCIDSFESLPNTELTQSDTAAILYSSGTTGTSKGVILTHANLISVMELLI 209
             LS + ++E   +     P  E+ QSDTAA+LYSSGTTGTSKGV LTH N I+   ++ 
Sbjct: 165 KILSFDNVMELS-EPVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVT 224

Query: 210 WYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAIETFEVN 269
              D     + VFLCF+PMFH++GL         RG   V M RF  + ++  IE F V 
Sbjct: 225 MDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVT 284

Query: 270 NVPAVPPVILGLVKSG--DGSDFSSLRRIGSGAAPLEK 298
           ++  VPPV L L K       D SSL+ IGSGAAPL K
Sbjct: 285 HLWVVPPVFLALSKQSIVKKFDLSSLKYIGSGAAPLGK 321

[Close]

BLAST of Sgr018807 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 134.0 bits (336), Expect = 2.2e-31
Identity = 93/295 (31.53%), Postives = 142/295 (48.14%), Query Frame = 0


Query: 54  PDLDTATFVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------ 113
           P LD  TF+ SQ  +   R+A ID+ T   +T+ +L                        
Sbjct: 30  PSLDVTTFISSQAHR--GRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVVLL 89

Query: 114 --------------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTG 173
                               IITT NP+NT++EIAKQ++DS   LA +  + L K+    
Sbjct: 90  LSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAA 149

Query: 174 --VPTILTTRPSYGDDLSVEELIECC-IDSFESLPNTELTQSDTAAILYSSGTTGTSKGV 233
             +P +L           V  L+E    +   +     + Q DTA +LYSSGTTG SKGV
Sbjct: 150 KKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGV 209

Query: 234 ILTHANLISVMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRF 293
           I +H NLI++++ ++    +   +   F+C +PMFHIYGL  FA GL   G+T +++ +F
Sbjct: 210 ISSHRNLIAMVQTIVNRFGSDDGEQR-FICTVPMFHIYGLAAFATGLLAYGSTIIVLSKF 269

Query: 294 NFQAMIDAIETFEVNNVPAVPPVILGLVKSGD----GSDFSSLRRIGSGAAPLEK 298
               M+ AI  ++  ++P VPP+++ +V   D      D SS+  +  G APL K
Sbjct: 270 EMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSK 321

[Close]

BLAST of Sgr018807 vs. TAIR 10
Match: AT1G20510.2 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 134.0 bits (336), Expect = 2.2e-31
Identity = 93/295 (31.53%), Postives = 142/295 (48.14%), Query Frame = 0


Query: 54  PDLDTATFVLSQFPKAESRVALIDSVTSSRVTYGQL------------------------ 113
           P LD  TF+ SQ  +   R+A ID+ T   +T+ +L                        
Sbjct: 30  PSLDVTTFISSQAHR--GRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVVLL 89

Query: 114 --------------------IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTG 173
                               IITT NP+NT++EIAKQ++DS   LA +  + L K+    
Sbjct: 90  LSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAA 149

Query: 174 --VPTILTTRPSYGDDLSVEELIECC-IDSFESLPNTELTQSDTAAILYSSGTTGTSKGV 233
             +P +L           V  L+E    +   +     + Q DTA +LYSSGTTG SKGV
Sbjct: 150 KKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGV 209

Query: 234 ILTHANLISVMELLIWYVDASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRF 293
           I +H NLI++++ ++    +   +   F+C +PMFHIYGL  FA GL   G+T +++ +F
Sbjct: 210 ISSHRNLIAMVQTIVNRFGSDDGEQR-FICTVPMFHIYGLAAFATGLLAYGSTIIVLSKF 269

Query: 294 NFQAMIDAIETFEVNNVPAVPPVILGLVKSGD----GSDFSSLRRIGSGAAPLEK 298
               M+ AI  ++  ++P VPP+++ +V   D      D SS+  +  G APL K
Sbjct: 270 EMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSK 321

[Close]

BLAST of Sgr018807 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 130.6 bits (327), Expect = 2.4e-30
Identity = 76/217 (35.02%), Postives = 120/217 (55.30%), Query Frame = 0


Query: 90  IITTANPINTASEIAKQVRDSGAKLAVSAPEELHKLVPTGVPTILTTRPSYGDDLSVEEL 149
           + TTAN +NT+ EI+KQ+ DS   L V    +L   +P  +  +LT    Y +  S   +
Sbjct: 115 VFTTANTLNTSGEISKQIADSNPTL-VFTTRQLAPKLPVAISVVLTDDEVYQELTSAIRV 174

Query: 150 IECCIDSFESLPNTE-----LTQSDTAAILYSSGTTGTSKGVILTHANLISVMELLIWYV 209
           +    +  +  P+ +     + Q DTA +LYSSGTTG SKGVI +H NL + +     ++
Sbjct: 175 VGILSEMVKKEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVAR---FI 234

Query: 210 DASCSQNDVFLCFIPMFHIYGLVFFALGLFCRGTTTVLMQRFNFQAMIDAIETFEVNNVP 269
             +  ++D+F+C +PMFH YGL+ FA+G    G+T V+++RF    M+DA+E      + 
Sbjct: 235 SDNLKRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALA 294

Query: 270 AVPPVILGLVKSGD----GSDFSSLRRIGSGAAPLEK 298
             PPV++ ++   D      D SSL+ +  G APL K
Sbjct: 295 LAPPVLVAMINDADLIKAKYDLSSLKTVRCGGAPLSK 327

The following BLAST results are available for this feature:

BLAST of Sgr018807 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138968.1	2.8e-126	74.64	4-coumarate--CoA ligase-like 5 [Momordica charantia]	[more]
XP_023523152.1	1.9e-122	69.71	4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7031989.1	2.8e-121	69.43	4-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022957594.1	8.0e-121	69.71	4-coumarate--CoA ligase 1-like [Cucurbita moschata]	[more]
XP_022994494.1	3.0e-120	69.14	4-coumarate--CoA ligase-like 5 [Cucurbita maxima]	[more]

BLAST of Sgr018807 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
M4IRL6	2.4e-35	41.28	Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1	[more]
Q84P24	1.3e-33	38.89	4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=...	[more]
Q9M0X9	8.6e-33	40.83	4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=...	[more]
M4IQR7	2.5e-32	37.95	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]
Q336M7	1.6e-31	35.94	4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2...	[more]

BLAST of Sgr018807 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CEK1	1.4e-126	74.64	4-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE...	[more]
A0A6J1H2E6	3.9e-121	69.71	4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=...	[more]
A0A6J1K303	1.5e-120	69.14	4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 ...	[more]
A0A5A7SZP5	1.6e-114	67.24	4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S4DW24	1.6e-114	67.24	4-coumarate--CoA ligase-like 5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10348942...	[more]

BLAST of Sgr018807 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19010.1	9.4e-35	38.89	AMP-dependent synthetase and ligase family protein	[more]
AT4G05160.1	6.1e-34	40.83	AMP-dependent synthetase and ligase family protein	[more]
AT1G20510.1	2.2e-31	31.53	OPC-8:0 CoA ligase1	[more]
AT1G20510.2	2.2e-31	31.53	OPC-8:0 CoA ligase1	[more]
AT1G20500.1	2.4e-30	35.02	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 89..300 e-value: 3.8E-35 score: 121.3
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 44..315 e-value: 1.6E-51 score: 177.4
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 22..89
None	No IPR available	PANTHER	PTHR24096:SF376	AMP-DEPENDENT SYNTHETASE AND LIGASE FAMILY PROTEIN-RELATED	coord: 90..298 coord: 22..89
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 90..298
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 90..298
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 173..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr018807.1	Sgr018807.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016405	CoA-ligase activity