Sgr018802 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr018802 vs. NCBI nr
Match: XP_038892369.1 (pullulanase 1, chloroplastic [Benincasa hispida] >XP_038892370.1 pullulanase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1641.3 bits (4249), Expect = 0.0e+00
Identity = 813/966 (84.16%), Postives = 850/966 (87.99%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MSLLFSSSLHLLPSSTP TS  Y   PRIAAFSSSPPAGG FS + TS S + DD  R G
Sbjct: 1   MSLLFSSSLHLLPSSTPATSICYQAHPRIAAFSSSPPAGGFFSVSATSLSHFLDDNFRSG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
            LY SSSSSMSLE+STST+QLEDSLLYSRAFWVS+Y+IAWNVEVGSGSCYLFASKMATLR
Sbjct: 61  SLYCSSSSSMSLEESTSTSQLEDSLLYSRAFWVSRYVIAWNVEVGSGSCYLFASKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           ++DGVVEG+DVK+KLEKDRG LPENVIKKFPHIQNY PFTVP ASDVE LLKCQLAVATF
Sbjct: 121 IEDGVVEGYDVKLKLEKDRGRLPENVIKKFPHIQNYCPFTVPLASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           NSYG+CNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAV+AQI+RDPVGG
Sbjct: 181 NSYGECNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
            PFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQ+EKCFT+DPY+RGISSDGR
Sbjct: 241 TPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQIEKCFTTDPYSRGISSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVD+GS+DL P+GWDKLADEK  V SFSDISIYELH+RD+SISDQSVHPDLRGG+MA
Sbjct: 301 RTLFVDIGSNDLIPKGWDKLADEKHPVDSFSDISIYELHVRDFSISDQSVHPDLRGGFMA 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLGEVY-------SLPQY 420
           FTLQDSAGI HLKKLSNAGISHVHLLPTFQF GVDDDKTKWK             S  Q 
Sbjct: 361 FTLQDSAGINHLKKLSNAGISHVHLLPTFQFGGVDDDKTKWKFFDTELLEDLPPDSAEQE 420

Query: 421 PLLLQC--------RYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+            YNPILWGVPKGSYASDPNGPCR+IEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADILNSDGYNWGYNPILWGVPKGSYASDPNGPCRVIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHGHGPFD NSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGHGPFDPNSVLDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMK TMLKAKDALQ LTKEKNGVDGSSIYIYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+VAE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKVAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET------- 720
            MLAVSKDHI+ AMAANLRDYVLTNF+G EV G EVLTHDGS VAYASCP+ET       
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYVLTNFEGQEVKGLEVLTHDGSPVAYASCPSETVNYVSAH 720

Query: 721 -------------------------------------GIPFFHCGDELLRSKSMDRDSYN 780
                                                GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTPRNIAVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNL 840
           SGDWFNRLDFTY TNNWGVGLPPKEKNE NWPLIKPRLADPSFKPSK+HILAAVEN TNL
Sbjct: 781 SGDWFNRLDFTYTTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKTHILAAVENCTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYI 900
           LQIRYSSPLFRLKT+NAIQ+RVRFHN+GASLIPG+IVMSIEDGHEGIPGLSQLD IYSYI
Sbjct: 841 LQIRYSSPLFRLKTSNAIQRRVRFHNSGASLIPGLIVMSIEDGHEGIPGLSQLDSIYSYI 900

Query: 901 VVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPSRTTSVFV 908
           VVVVNARPTE+SFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVP RTT+VFV
Sbjct: 901 VVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPPRTTAVFV 960

BLAST of Sgr018802 vs. NCBI nr
Match: XP_022945721.1 (pullulanase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1632.1 bits (4225), Expect = 0.0e+00
Identity = 808/966 (83.64%), Postives = 845/966 (87.47%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MS LFSSSLHLLP S P TS S+HLPPRIAA SSSP  GG+FS A TS S YP  + R G
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFSVAATSLSHYPHGHSRCG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
           PLY SSS+SMSLE+ST  +QLEDSLLY+RAFWVS+Y+IAWNVEVGSGSCYLF SKMATLR
Sbjct: 61  PLYCSSSASMSLEESTPASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           V DGVVEG+DVKIKLEKDRGPLPENVIKKFPHIQNY PFTVP  S VE LLKCQLAVATF
Sbjct: 121 VNDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIY++PVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
            PFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTL+VEKCF++DPY+RG+SSDGR
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVDLGSDDLKPEGWDKLADEKP +HSFSDISIYELH+RD+SISDQSVHPD+RGGYM 
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDMRGGYMG 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLG-------EVYSLPQY 420
           FTLQDS GITHLKKLSNAGISHVHLLPTFQF GVDDDK+KWKSL           S  Q 
Sbjct: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 PLLLQCR--------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+   +        YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHG GPFD +SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM  TMLKAK+ALQ LT+EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+ AE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET------- 720
           F LAVSKDHIQ AMAANL+DYVL NF+G EV GSEV T+DGSSVAYASCPTET       
Sbjct: 661 FTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720

Query: 721 -------------------------------------GIPFFHCGDELLRSKSMDRDSYN 780
                                                GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNL 840
           SGDWFNRLDFTYMTNNWGVGLPPKEKNE NWPLIKPRLADPSFKPSKSHILAAVEN TNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYI 900
           LQIRYSSPLFRLKTANAIQKRVRFHNTG SLIPGVIVMSIEDG EGIPGL+QLDPIYSYI
Sbjct: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900

Query: 901 VVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPSRTTSVFV 908
           VVVVNARP+E+SFPCPALRARTLQLHPIQLMSTDP+VKNS YEPSTGCFMVPSRTTSVFV
Sbjct: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960

BLAST of Sgr018802 vs. NCBI nr
Match: KAG6573853.1 (Pullulanase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1626.3 bits (4210), Expect = 0.0e+00
Identity = 806/966 (83.44%), Postives = 842/966 (87.16%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MS LFSSSLHLLP S P TS S+HLPPRIAA SSSP  GG+FS A TS S YPD + R G
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFSVAATSLSHYPDGHSRCG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
           PLY SSS+SMSLE+ST  +QLEDSLLY+RAFWVS+Y+IAWNVEV SGSCYLF SKMATLR
Sbjct: 61  PLYCSSSASMSLEESTPASQLEDSLLYARAFWVSRYVIAWNVEVRSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           V DGVVEG+DVKIKLEKD GPLPENVIKKFPHIQNY PFTVP  S VE LLKCQLAVATF
Sbjct: 121 VNDGVVEGYDVKIKLEKDSGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAVQAQIY++PVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEVVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
            PFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTL+VEKCF++DPY+RG+SSDGR
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVDLGSDDLKPEGWDKLADEKP +HSFSDISIYELH+RD+SISDQSVHPD+RGGYM 
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDMRGGYMG 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLG-------EVYSLPQY 420
           FTLQDS GITHLKKLSNAGISHVHLLPTFQF GVDDDK+KWKSL           S  Q 
Sbjct: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 PLLLQCR--------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+   +        YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHG GPFD  SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPFDPISVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM  TMLKAK+ALQ LT+EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+ AE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET------- 720
           F LAVSKDHIQ AMAANL+DYVL NF+G EV GSEV T+DGSSVAYASCPTET       
Sbjct: 661 FTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720

Query: 721 -------------------------------------GIPFFHCGDELLRSKSMDRDSYN 780
                                                GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNL 840
           SGDWFNRLDFTYMTNNWGVGLPPKEKNE NWPLIKPRLADPSFKPSKSHILAAVEN TNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYI 900
           LQIRYSSPLFRLKTANAIQKRVRFHNTG SLIPGVIVMSIEDG EGIPGL+QLDPIYSYI
Sbjct: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900

Query: 901 VVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPSRTTSVFV 908
           VVVVNARP+E+SFPCPALRARTLQLHPIQLMSTDP+VKNS YEPSTGCFMVPSRTTSVFV
Sbjct: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960

BLAST of Sgr018802 vs. NCBI nr
Match: XP_022966985.1 (LOW QUALITY PROTEIN: pullulanase 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1624.4 bits (4205), Expect = 0.0e+00
Identity = 798/923 (86.46%), Postives = 839/923 (90.90%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MS LFSSSLHLLP S P TS  ++LPPRIAA  SSPPAGG+FS A TS S YPD + R G
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFRHNLPPRIAAVPSSPPAGGIFSVAATSLSHYPDGHSRCG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
           PLY SSS+SMS+E+STS +QLEDSLLY+RAFWVS+Y+IAWNVEVGSGSCYLF SKMATLR
Sbjct: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           VKDGVVEG+DVKIKLEKDRGPLPENVIKKFPHIQNY PFTVP  S VE LLKCQLAVATF
Sbjct: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIY++PVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
            PFEVIQLEELDGVWRTKGPKSW+GCYYEYEVTVYHPSTL+VEKCF++DPY+RG+SSDG 
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWDGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGLSSDGG 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVDLGSDDLKPEGWDKLADEKP +HSFSDISIYELH+RD+SISDQSVHPDLRGGYM 
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLG-------EVYSLPQY 420
           F+LQDS GITHLKKLSNAGISHVHLLPTFQF GVDDDK+KWKSL           S  Q 
Sbjct: 361 FSLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 PLLLQCR--------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+   +        YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHG GP D +SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPLDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM  TMLKAK+ALQ LT EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTLEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+ AE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAY-ASCPTETGIPFFH 720
           F LA+SKDHIQ AMAANLRDYVLTNF+G EV GSE+ T+DGSSVAY  +     GIPFFH
Sbjct: 661 FTLAMSKDHIQVAMAANLRDYVLTNFEGKEVKGSELRTYDGSSVAYXITLFLFKGIPFFH 720

Query: 721 CGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSF 780
           CGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNE NWPLIKPRLADPSF
Sbjct: 721 CGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSF 780

Query: 781 KPSKSHILAAVENLTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDG 840
           KPSKSHILAAVEN TNLLQIRYSSPLFRLKTANAIQKRVRFHNTG SLIPGVIVMSIEDG
Sbjct: 781 KPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDG 840

Query: 841 HEGIPGLSQLDPIYSYIVVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYE 900
            EGIPGL+QLDPIYSYIVVVVNARP+E+SFPCP LRARTLQLHPIQLMSTDP+VKNS YE
Sbjct: 841 QEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAYE 900

Query: 901 PSTGCFMVPSRTTSVFVEPRMHE 908
           PSTGCFMVPSRTTSVFVE R HE
Sbjct: 901 PSTGCFMVPSRTTSVFVELRXHE 923

BLAST of Sgr018802 vs. NCBI nr
Match: XP_004142505.1 (pullulanase 1, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 1596.3 bits (4132), Expect = 0.0e+00
Identity = 796/966 (82.40%), Postives = 835/966 (86.44%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MSLLFSSSLH  P S   TS  Y L    AAFSSSPPA G      TS SP      R G
Sbjct: 1   MSLLFSSSLHFFPPSAFPTSLCYQLHHPFAAFSSSPPAAG------TSLSPGFRHNFRTG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
           PLY S SSSMSLE+STST+QLEDSLLYSRAFWVSK++IAWNVEV SGSCYLFASKMATLR
Sbjct: 61  PLYCSPSSSMSLEESTSTSQLEDSLLYSRAFWVSKHVIAWNVEVESGSCYLFASKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           V+DGVVEG+DVKI+LEKDR  LPENVIKKFPHIQNY PFTVPPASDVE LLKCQLAVATF
Sbjct: 121 VEDGVVEGYDVKIRLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           NSYG+C NITCLQLPGVLDDLFSYEGPLGA+YSKEAVSLYLWAPTAQAV+AQI+RDPVGG
Sbjct: 181 NSYGECKNITCLQLPGVLDDLFSYEGPLGAIYSKEAVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
           MPFEVI LEE+DG+WRTKGPKSW+GCYYEYEVTVYHPSTLQVEKCFT+DPY+RG+SSDGR
Sbjct: 241 MPFEVIPLEEVDGIWRTKGPKSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVDL SDDL P+GWDKLADEKP V SFSDISIYELH+RD+SISDQSVHPDLRGGYMA
Sbjct: 301 RTLFVDLFSDDLIPKGWDKLADEKPPVDSFSDISIYELHVRDFSISDQSVHPDLRGGYMA 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLGE--VYSLP-----QY 420
           FTLQDSAGI HLKKLSNAG+SHVHLLPTFQF GVDDDKTKWK +    + +LP     Q 
Sbjct: 361 FTLQDSAGINHLKKLSNAGLSHVHLLPTFQFGGVDDDKTKWKFVDTELLENLPPDSAEQQ 420

Query: 421 PLLLQCR--------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+   +        YNPI+WGVPKGSYASD NGPCRL+EFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHGHGPFD NSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMK TMLKAKDAL+ LTKEKNGVDGSSIYIYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGT EVAE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTHEVAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET------- 720
            MLAVSKDHI+ AMAANLRDY+LTNF+G EV G EVLTHD S VAYASCPTET       
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVSAH 720

Query: 721 -------------------------------------GIPFFHCGDELLRSKSMDRDSYN 780
                                                GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNL 840
           SGDWFNRLDFTYMTNNWGVGLP KEKN+ NWPLIKPRLADPSFKPSKSHILAAVEN TNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYI 900
           LQIRYSSPLFRLKT+NAIQKRVRFHN+G SLIPG+IVMSIEDGH GIPGLSQLD  YSYI
Sbjct: 841 LQIRYSSPLFRLKTSNAIQKRVRFHNSGTSLIPGLIVMSIEDGHNGIPGLSQLDSTYSYI 900

Query: 901 VVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPSRTTSVFV 908
           VVVVNARPTE+SFPCPALRA+TLQLHPIQLMSTDP+VKNSTYEPSTGCFMVP RTTSVFV
Sbjct: 901 VVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTYEPSTGCFMVPPRTTSVFV 960

BLAST of Sgr018802 vs. ExPASy Swiss-Prot
Match: Q8GTR4 (Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2)

HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 568/899 (63.18%), Postives = 687/899 (76.42%), Query Frame = 0

Query: 65  SSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLRVKDG 124
           S+SS MSLE S+  +Q  D L+YSRA+WV++ +IAWNV+VG GSCY +ASK A L   + 
Sbjct: 65  SASSPMSLEVSSPNSQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSED 124

Query: 125 VVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATFNSYG 184
            ++G+D++IKLE + G LP +VI+KFPHI+NY  F VP   D+  L+K QLAV  F++ G
Sbjct: 125 GIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEG 184

Query: 185 QCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGGMPFE 244
           +    T LQLPGVLD+LFSY+GPLGA ++ E VSL+LWAPTAQAV   IY++P+   P E
Sbjct: 185 RLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPME 244

Query: 245 VIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGRRTLF 304
           +  L+E +GVW T+G  SW GCYY Y+V+VYHPST+++E C+ +DPYARG+S+DGR+T  
Sbjct: 245 ICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTFL 304

Query: 305 VDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMAFTLQ 364
           V+L SDDLKPEGWD LAD+KP + SFSDISIYELH+RD+S +D++V P+ RGGY+AFT +
Sbjct: 305 VNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSK 364

Query: 365 DSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLGE--VYSLPQYPLLLQCR-- 424
           DSAG+ HL+KL +AG++H+HLLPTFQF  VDD+K  WKS+    +  L       Q R  
Sbjct: 365 DSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARIT 424

Query: 425 -----------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHL 484
                      YNP+LWGVPKGSYASDP GPCR+IEFRKMVQALN  GL VVLDVVYNHL
Sbjct: 425 EIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHL 484

Query: 485 HGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDY 544
           H  GP D  SVLDKIVPGYYLRRN+DGFIENSTCVNNTASEH+MV+R+I DD+L+WVV+Y
Sbjct: 485 HASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNY 544

Query: 545 KVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNGRGVN 604
           KVDGFRFDLMGH+MK T++ AK A+ SL KE +GVDGS IY+YGEGW+FGEVA+NGRG+N
Sbjct: 545 KVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGIN 604

Query: 605 ASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFMLA 664
           ASQFNL GTGIGSFNDR+RDA LGGSPFGHPLQQGF+TGLLL+PN HDHG++   E ML+
Sbjct: 605 ASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLS 664

Query: 665 VSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET----------- 724
            +K+HIQ  MAANL+DY+LTN +G EV GSEVL HD + VAYAS PTET           
Sbjct: 665 TAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNET 724

Query: 725 ---------------------------------GIPFFHCGDELLRSKSMDRDSYNSGDW 784
                                            GIPFFH GDE+LRSKS+DRDSYNSGDW
Sbjct: 725 LFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDW 784

Query: 785 FNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNLLQIR 844
           FNRLDF+Y +NNWGVGLPPK KNE NWPLIKPRL DPSFKP  SHI+A + N  +LL+IR
Sbjct: 785 FNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIR 844

Query: 845 YSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYIVVVV 904
           YSSPLFRL TA AIQ+RVRFHNTG S IPG IVMSIEDGH GIP +SQ+DPIYS IVV+ 
Sbjct: 845 YSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIF 904

BLAST of Sgr018802 vs. ExPASy Swiss-Prot
Match: P07206 (Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2)

HSP 1 Score: 455.3 bits (1170), Expect = 1.6e-126
Identity = 302/922 (32.75%), Postives = 453/922 (49.13%), Query Frame = 0

Query: 88   SRAFWVSKYIIAWNVEVGSGSCYLFASKMATLRVKDGVVEGHDVKIKLEKDRGPLPENVI 147
            + A WV K  + W          L+ S  + +   DG  +  D  +KL      + + V 
Sbjct: 184  AEAHWVDKNTLLWPGGQDKPIVRLYYSHSSKV-AADGEGKFTDRYLKLTPT--TVSQQVS 243

Query: 148  KKFPHIQNYSPFTVPPASDVETLLKCQLAVATFNSYGQCNNITCLQLPGVLDDLFSYEG- 207
             +FPH+ +Y+ F +P  ++V+ LL+ +         G   + T +Q  GVLDD ++    
Sbjct: 244  MRFPHLSSYAAFKLPDNANVDELLQGETVAIAAAEDGILISATQVQTAGVLDDAYAEAAE 303

Query: 208  --PLGAVYSKEAVSLYLWAPTAQAVQAQIY---RDPVGGMPFEVIQLEELDGVWRTKGPK 267
                GA  +   V+  +WAPTAQ V   +Y   +  +G  P   +  +   G W  +G  
Sbjct: 304  ALSYGAQLADGGVTFRVWAPTAQQVDVVVYSADKKVIGSHP---MTRDSASGAWSWQGGS 363

Query: 268  SWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGRRTLFVDLGSDDLKPEGWDKLA 327
              +G +Y Y +TVYHP + +VE+   +DPYA  +S++   +  VDL    LKP+GWD L 
Sbjct: 364  DLKGAFYRYAMTVYHPQSRKVEQYEVTDPYAHSLSTNSEYSQVVDLNDSALKPDGWDNLT 423

Query: 328  --DEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMAFTLQDSAGITHLKKLSNAG 387
                +      + ++I+E HIRD S  DQ+V  +LRG Y+A T  DS  + HLK LS +G
Sbjct: 424  MPHAQKTKADLAKMTIHESHIRDLSAWDQTVPAELRGKYLALTAGDSNMVQHLKTLSASG 483

Query: 388  ISHVHLLPTFQFAGVDDDKTKWKSLGEVYSL----------------------------- 447
            ++HV LLP F  A V++   K   + + +S                              
Sbjct: 484  VTHVELLPVFDLATVNEFSDKVADIQQPFSRLCEVNSAVKSSEFAGYCDSGSTVEEVLNQ 543

Query: 448  ---------PQYPLL---------LQCRYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 507
                     PQ   L             Y+P  + VP+GSYA+DP G  R+ EFR M+QA
Sbjct: 544  LKQSDSQDNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDPEGTTRIKEFRTMIQA 603

Query: 508  LNQ-IGLRVVLDVVYNHLHGHGPFDLNSVLDKIVPGYYLRRN-TDGFIENSTCVNNTASE 567
            + Q +G+ V++DVVYNH +  GP D  SVLDKIVP YY R N T G +E++TC +++A E
Sbjct: 604  IKQDLGMNVIMDVVYNHTNAAGPTDRTSVLDKIVPWYYQRLNETTGSVESATCCSDSAPE 663

Query: 568  HFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIY 627
            H M  ++I D +  W  DYK+DGFRFDLMG+  K  +L A + +++L  +        IY
Sbjct: 664  HRMFAKLIADSLAVWTTDYKIDGFRFDLMGYHPKAQILSAWERIKALNPD--------IY 723

Query: 628  IYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPF--GHPLQ--QGFV 687
             +GEGWD  +   + R   ASQ NL GTGIG+F+DR+RD++ GG PF  G  L+  QG  
Sbjct: 724  FFGEGWDSNQ---SDRFEIASQINLKGTGIGTFSDRLRDSVRGGGPFDSGDALRQNQGIG 783

Query: 688  TGLLLEPNDHDHGTDEVAEFMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDG 747
            +G  + PN+    +D+    +     D  +  MA NL D+V+ +  G    GSE+  ++G
Sbjct: 784  SGAGVLPNELASLSDDQVRHL----ADLTRLGMAGNLADFVMIDKDGAAKKGSEI-DYNG 843

Query: 748  SSVAYASCPTET--------------------------------------------GIPF 807
            +   YA+ PTE                                             GI F
Sbjct: 844  APGGYAADPTEVVNYVSKHDNQTLWDMISYKASQEADLATRVRMQAVSLATVMLGQGIAF 903

Query: 808  FHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADP 867
               G ELLRSKS  RDSY+SGDWFNR+D++   NN+ VG+P    +  N+ +I  R+ + 
Sbjct: 904  DQQGSELLRSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRISDDGSNYEVI-TRVKEM 963

Query: 868  SFKPSKSHILAAVENLTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIE 905
               P ++ +         L ++R SSPLF L   +A+ KRV F NTG+    G++VM+++
Sbjct: 964  VATPGEAELKQMTAFYQELTELRKSSPLFTLGDGSAVMKRVDFRNTGSDQQAGLLVMTVD 1023

BLAST of Sgr018802 vs. ExPASy Swiss-Prot
Match: P07811 (Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 6.6e-120
Identity = 285/845 (33.73%), Postives = 411/845 (48.64%), Query Frame = 0

Query: 90   AFWVSKYIIAWNVEVGSGSCYLFASKMATLRVKDGVVEGHDVKIKLEKDRGPLPENVIKK 149
            A WV K  + W          L+ S  + +   D   E  D  +KL      + + V  +
Sbjct: 197  AHWVDKTTLLWPGGENKPIVRLYYSHSSKV-AADSNGEFSDKYVKLTPT--TVNQQVSMR 256

Query: 150  FPHIQNYSPFTVPPASDVETLLKCQLAVATFNSYGQCNNITCLQLPGV-----------L 209
            FPH+ +Y  F +P   +V+ LL+            + + I  L  PG             
Sbjct: 257  FPHLASYPAFKLPDDVNVDELLQ-----GDDGGIAESDGILSLSHPGADRRRAGRYLCRR 316

Query: 210  DDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGGMPFEVIQLEELDGVWRTK 269
             +  SY    GA  +   V+  +WAPTAQ V+  IY      +    +  +   G W  +
Sbjct: 317  AEALSY----GAQLTDSGVTFRVWAPTAQQVELVIYSADKKVIASHPMTRDSASGAWSWQ 376

Query: 270  GPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGRRTLFVDLGSDDLKPEGWD 329
            G    +G +Y Y +TVYHP + +VE+   +DPYA  +S++   +  VDL    LKPEGWD
Sbjct: 377  GGSDLKGAFYRYAMTVYHPQSRKVEQYEVTDPYAHSLSTNSEYSQVVDLNDSALKPEGWD 436

Query: 330  KLA--DEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMAFTLQDSAGITHLKKLS 389
             L     +      + ++I+E HIRD S  DQ+V  +LRG Y+A T Q+S  + HLK+LS
Sbjct: 437  GLTMPHAQKTKADLAKMTIHESHIRDLSAWDQTVPAELRGKYLALTAQESNMVQHLKQLS 496

Query: 390  NAGISHVHLLPTFQFAGVDDDKTKWKSLGEVYSL-------------------------- 449
             +G++H+ LLP F  A V++   K   + + +S                           
Sbjct: 497  ASGVTHIELLPVFDLATVNEFSDKVADIQQPFSRLCEVNSAVKSSEFAGYCDSGSTVEEV 556

Query: 450  ------------PQYPLL---------LQCRYNPILWGVPKGSYASDPNGPCRLIEFRKM 509
                        PQ   L             Y+P  + VP+GSYA+DP G  R+ EFR M
Sbjct: 557  LTQLKQNDSKDNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDPEGTARIKEFRTM 616

Query: 510  VQALNQ-IGLRVVLDVVYNHLHGHGPFDLNSVLDKIVPGYYLRRN-TDGFIENSTCVNNT 569
            +QA+ Q +G+ V++DVVYNH +  GP D  SVLDKIVP YY R N T G +E++TC +++
Sbjct: 617  IQAIKQDLGMNVIMDVVYNHTNAAGPTDRTSVLDKIVPWYYQRLNETTGSVESATCCSDS 676

Query: 570  ASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGS 629
            A EH M  ++I D +  W  DYK+DGFRFDLMG+  K  +L A + +++L  +       
Sbjct: 677  APEHRMFAKLIADSLAVWTTDYKIDGFRFDLMGYHPKAQILSAWERIKALNPD------- 736

Query: 630  SIYIYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPF--GHPLQ--Q 689
             IY +GEGWD  +   + R   ASQ NL GTGIG+F+DR+RDA+ GG PF  G  L+  Q
Sbjct: 737  -IYFFGEGWDSNQ---SDRFEIASQINLKGTGIGTFSDRLRDAVRGGGPFDSGDALRQNQ 796

Query: 690  GFVTGLLLEPNDHDHGTDEVAEFMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLT 749
            G  +G  + PN+    +D+ A  +     D  +  MA NL D+VL +  G    GSE+  
Sbjct: 797  GVGSGAGVLPNELTTLSDDQARHL----ADLTRLGMAGNLADFVLIDKDGAVKRGSEI-D 856

Query: 750  HDGSSVAYASCPTET--------------------------------------------G 809
            ++G+   YA+ PTE                                             G
Sbjct: 857  YNGAPGGYAADPTEVVNYVSKHDNQTLWDMISYKAAQEADLDTRVRMQAVSLATVMLGQG 916

Query: 810  IPFFHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRL 825
            I F   G ELLRSKS  RDSY+SGDWFNR+D++   NN+ VG+P    +  N+ +I  R+
Sbjct: 917  IAFDQQGSELLRSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRSSDDGSNYDII-ARV 976

BLAST of Sgr018802 vs. ExPASy Swiss-Prot
Match: O33840 (Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2)

HSP 1 Score: 242.7 bits (618), Expect = 1.7e-62
Identity = 189/686 (27.55%), Postives = 315/686 (45.92%), Query Frame = 0

Query: 197 VLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGGMPFEVIQLE-ELDGVW 256
           +LDD + Y+G LGAVYS E     +W+P ++ V+  ++++     P++V+ +E + +GVW
Sbjct: 215 ILDDYY-YDGELGAVYSPEKTIFRVWSPVSKWVKVLLFKNGEDTEPYQVVNMEYKGNGVW 274

Query: 257 RTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGRRTLFVDLGSDDLKPE 316
                   +G +Y Y++  Y           T DPY++ + ++ +++  V+L   +  PE
Sbjct: 275 EAVVEGDLDGVFYLYQLENYGKIRT------TVDPYSKAVYANSKKSAVVNLARTN--PE 334

Query: 317 GWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMAFTLQDSAG----ITH 376
           GW+   D  P +  + D  IYE+HI D +  + S   + +G Y+  T +++ G     T 
Sbjct: 335 GWEN--DRGPKIEGYEDAIIYEIHIADITGLENSGVKN-KGLYLGLTEENTKGPGGVTTG 394

Query: 377 LKKLSNAGISHVHLLPTFQF---AGVDDDKTKWKSLGEVYSLPQYPLLLQCRYNPILWGV 436
           L  L   G++HVH+LP F F     +D D  K+ + G               Y+P L+ V
Sbjct: 395 LSHLVELGVTHVHILPFFDFYTGDELDKDFEKYYNWG---------------YDPYLFMV 454

Query: 437 PKGSYASDPNGP-CRLIEFRKMVQALNQIGLRVVLDVVYNHLHGHGPFDLNSVLDKIVPG 496
           P+G Y++DP  P  R+ E ++MV+AL++ G+ V++D+V+ H +G G     S  D+ VP 
Sbjct: 455 PEGRYSTDPKNPHTRIREVKEMVKALHKHGIGVIMDMVFPHTYGIGEL---SAFDQTVPY 514

Query: 497 YYLR-RNTDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKRT 556
           Y+ R   T  ++  S C N  ASE  M+ + IVD + +WV +Y +DGFRFD MG + K+T
Sbjct: 515 YFYRIDKTGAYLNESGCGNVIASERPMMRKFIVDTVTYWVKEYHIDGFRFDQMGLIDKKT 574

Query: 557 MLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDR 616
           ML+ + AL  +          +I +YGE W        G  +   + ++ GT + +FND 
Sbjct: 575 MLEVERALHKI--------DPTIILYGEPW-----GGWGAPIRFGKSDVAGTHVAAFNDE 634

Query: 617 VRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGT--------DEVAEFMLAVSKDHIQAA 676
            RDAI  GS F +P  +GFV G   +      G           +  F L   +    AA
Sbjct: 635 FRDAI-RGSVF-NPSVKGFVMGGYGKETKIKRGVVGSINYDGKLIKSFALDPEETINYAA 694

Query: 677 MAANL----RDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTETGIPFFHCGDELLRSKS 736
              N     ++Y+       +   +E    +   +A A   T  G+PF H G +  R+K+
Sbjct: 695 CHDNHTLWDKNYLAAK-ADKKKEWTEEELKNAQKLAGAILLTSQGVPFLHGGQDFCRTKN 754

Query: 737 MDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAA 796
            + +SYN+    N  D+                                    K   +  
Sbjct: 755 FNDNSYNAPISINGFDY----------------------------------ERKLQFIDV 805

Query: 797 VENLTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPG---VIVMSIEDGHEGIPGL 856
                 L+++R   P FRLK A  I+K + F       +PG   ++   ++D   G P  
Sbjct: 815 FNYHKGLIKLRKEHPAFRLKNAEEIKKHLEF-------LPGGRRIVAFMLKDHAGGDP-- 805

Query: 857 SQLDPIYSYIVVVVNARPTEVSFPCP 858
                 +  IVV+ N    + ++  P
Sbjct: 875 ------WKDIVVIYNGNLEKTTYKLP 805

BLAST of Sgr018802 vs. ExPASy Swiss-Prot
Match: C0SPA0 (Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.0e-59
Identity = 192/597 (32.16%), Postives = 279/597 (46.73%), Query Frame = 0

Query: 190 TCLQLPGVL-----DDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGGMPFE 249
           T LQ+  V+     DD F Y+G LGAVY+ +     +WAP A +   ++      G  F+
Sbjct: 83  TDLQIGAVIRTAAFDDEFYYDGELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQ 142

Query: 250 VIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGRRTLF 309
           + +LE+  GV+         G  YEY   + + S    E   T D YA+ ++ +G + + 
Sbjct: 143 MTRLEK--GVYAVTVTGDLHG--YEYLFCICNNS----EWMETVDQYAKAVTVNGEKGVV 202

Query: 310 VDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMAFTLQ 369
                  L+P+     A  KP  H   D  IYE H+RD+SI + S   + +G Y+A T  
Sbjct: 203 -------LRPDQMKWTAPLKPFSHPV-DAVIYETHLRDFSIHENSGMIN-KGKYLALTET 262

Query: 370 D-------SAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLGEVYSLPQYPLLL 429
           D       S+G+ ++K+L   G++HV LLP   FAGVD++K           L  Y    
Sbjct: 263 DTQTANGSSSGLAYVKEL---GVTHVELLPVNDFAGVDEEK----------PLDAY---- 322

Query: 430 QCRYNPILWGVPKGSYASDPNGP-CRLIEFRKMVQALNQIGLRVVLDVVYNHLHGHGPFD 489
              YNP+ +  P+GSYAS+P+ P  R  E ++M+  L+Q GLRV+LDVV+NH++      
Sbjct: 323 NWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRE--- 382

Query: 490 LNSVLDKIVPGYYLRRNTDGFIENSTCV-NNTASEHFMVERMIVDDILHWVVDYKVDGFR 549
            NS  +K VPGY+ R +  G   N T V N+ ASE  M  + I D +++W+ +Y VDGFR
Sbjct: 383 -NSPFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFR 442

Query: 550 FDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNGRG---VNASQ 609
           FDL+G L   T+L  K   +  TK K G     I ++GEGWD      + +     NA +
Sbjct: 443 FDLLGILDIDTVLYMK---EKATKAKPG-----ILLFGEGWDLATPLPHEQKAALANAPR 502

Query: 610 FNLFGTGIGSFNDRVRDAILGGS----PFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFML 669
                 GIG FND  RDA+ G +      G  L  G     ++       G   +A  + 
Sbjct: 503 M----PGIGFFNDMFRDAVKGNTFHLKATGFALGNGESAQAVMHGIAGSSGWKALAPIVP 562

Query: 670 AVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTETGIPFFHCGDE 729
             S+         N   +   +F   + N S         +A A      G+PF H G E
Sbjct: 563 EPSQSINYVESHDNHTFWDKMSFALPQENDSR--KRSRQRLAAAIILLAQGVPFIHSGQE 620

Query: 730 LLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFK 766
             R+K    +SY S D  N+LD       W      KE       LI  R A P+F+
Sbjct: 623 FFRTKQGVENSYQSSDSINQLD-------WDRRETFKEDVHYIRRLISLRKAHPAFR 620

BLAST of Sgr018802 vs. ExPASy TrEMBL
Match: A0A6J1G1P3 (pullulanase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449862 PE=4 SV=1)

HSP 1 Score: 1632.1 bits (4225), Expect = 0.0e+00
Identity = 808/966 (83.64%), Postives = 845/966 (87.47%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MS LFSSSLHLLP S P TS S+HLPPRIAA SSSP  GG+FS A TS S YP  + R G
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFSVAATSLSHYPHGHSRCG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
           PLY SSS+SMSLE+ST  +QLEDSLLY+RAFWVS+Y+IAWNVEVGSGSCYLF SKMATLR
Sbjct: 61  PLYCSSSASMSLEESTPASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           V DGVVEG+DVKIKLEKDRGPLPENVIKKFPHIQNY PFTVP  S VE LLKCQLAVATF
Sbjct: 121 VNDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIY++PVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
            PFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTL+VEKCF++DPY+RG+SSDGR
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVDLGSDDLKPEGWDKLADEKP +HSFSDISIYELH+RD+SISDQSVHPD+RGGYM 
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDMRGGYMG 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLG-------EVYSLPQY 420
           FTLQDS GITHLKKLSNAGISHVHLLPTFQF GVDDDK+KWKSL           S  Q 
Sbjct: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 PLLLQCR--------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+   +        YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHG GPFD +SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM  TMLKAK+ALQ LT+EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+ AE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET------- 720
           F LAVSKDHIQ AMAANL+DYVL NF+G EV GSEV T+DGSSVAYASCPTET       
Sbjct: 661 FTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720

Query: 721 -------------------------------------GIPFFHCGDELLRSKSMDRDSYN 780
                                                GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNL 840
           SGDWFNRLDFTYMTNNWGVGLPPKEKNE NWPLIKPRLADPSFKPSKSHILAAVEN TNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYI 900
           LQIRYSSPLFRLKTANAIQKRVRFHNTG SLIPGVIVMSIEDG EGIPGL+QLDPIYSYI
Sbjct: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900

Query: 901 VVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPSRTTSVFV 908
           VVVVNARP+E+SFPCPALRARTLQLHPIQLMSTDP+VKNS YEPSTGCFMVPSRTTSVFV
Sbjct: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960

BLAST of Sgr018802 vs. ExPASy TrEMBL
Match: A0A6J1HT34 (LOW QUALITY PROTEIN: pullulanase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466529 PE=4 SV=1)

HSP 1 Score: 1624.4 bits (4205), Expect = 0.0e+00
Identity = 798/923 (86.46%), Postives = 839/923 (90.90%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MS LFSSSLHLLP S P TS  ++LPPRIAA  SSPPAGG+FS A TS S YPD + R G
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFRHNLPPRIAAVPSSPPAGGIFSVAATSLSHYPDGHSRCG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
           PLY SSS+SMS+E+STS +QLEDSLLY+RAFWVS+Y+IAWNVEVGSGSCYLF SKMATLR
Sbjct: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           VKDGVVEG+DVKIKLEKDRGPLPENVIKKFPHIQNY PFTVP  S VE LLKCQLAVATF
Sbjct: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIY++PVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
            PFEVIQLEELDGVWRTKGPKSW+GCYYEYEVTVYHPSTL+VEKCF++DPY+RG+SSDG 
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWDGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGLSSDGG 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVDLGSDDLKPEGWDKLADEKP +HSFSDISIYELH+RD+SISDQSVHPDLRGGYM 
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLG-------EVYSLPQY 420
           F+LQDS GITHLKKLSNAGISHVHLLPTFQF GVDDDK+KWKSL           S  Q 
Sbjct: 361 FSLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 PLLLQCR--------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+   +        YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHG GP D +SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPLDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM  TMLKAK+ALQ LT EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTLEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+ AE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAY-ASCPTETGIPFFH 720
           F LA+SKDHIQ AMAANLRDYVLTNF+G EV GSE+ T+DGSSVAY  +     GIPFFH
Sbjct: 661 FTLAMSKDHIQVAMAANLRDYVLTNFEGKEVKGSELRTYDGSSVAYXITLFLFKGIPFFH 720

Query: 721 CGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSF 780
           CGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNE NWPLIKPRLADPSF
Sbjct: 721 CGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSF 780

Query: 781 KPSKSHILAAVENLTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDG 840
           KPSKSHILAAVEN TNLLQIRYSSPLFRLKTANAIQKRVRFHNTG SLIPGVIVMSIEDG
Sbjct: 781 KPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDG 840

Query: 841 HEGIPGLSQLDPIYSYIVVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYE 900
            EGIPGL+QLDPIYSYIVVVVNARP+E+SFPCP LRARTLQLHPIQLMSTDP+VKNS YE
Sbjct: 841 QEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAYE 900

Query: 901 PSTGCFMVPSRTTSVFVEPRMHE 908
           PSTGCFMVPSRTTSVFVE R HE
Sbjct: 901 PSTGCFMVPSRTTSVFVELRXHE 923

BLAST of Sgr018802 vs. ExPASy TrEMBL
Match: A0A0A0KTI2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622510 PE=4 SV=1)

HSP 1 Score: 1596.3 bits (4132), Expect = 0.0e+00
Identity = 796/966 (82.40%), Postives = 835/966 (86.44%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MSLLFSSSLH  P S   TS  Y L    AAFSSSPPA G      TS SP      R G
Sbjct: 1   MSLLFSSSLHFFPPSAFPTSLCYQLHHPFAAFSSSPPAAG------TSLSPGFRHNFRTG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
           PLY S SSSMSLE+STST+QLEDSLLYSRAFWVSK++IAWNVEV SGSCYLFASKMATLR
Sbjct: 61  PLYCSPSSSMSLEESTSTSQLEDSLLYSRAFWVSKHVIAWNVEVESGSCYLFASKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           V+DGVVEG+DVKI+LEKDR  LPENVIKKFPHIQNY PFTVPPASDVE LLKCQLAVATF
Sbjct: 121 VEDGVVEGYDVKIRLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           NSYG+C NITCLQLPGVLDDLFSYEGPLGA+YSKEAVSLYLWAPTAQAV+AQI+RDPVGG
Sbjct: 181 NSYGECKNITCLQLPGVLDDLFSYEGPLGAIYSKEAVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
           MPFEVI LEE+DG+WRTKGPKSW+GCYYEYEVTVYHPSTLQVEKCFT+DPY+RG+SSDGR
Sbjct: 241 MPFEVIPLEEVDGIWRTKGPKSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVDL SDDL P+GWDKLADEKP V SFSDISIYELH+RD+SISDQSVHPDLRGGYMA
Sbjct: 301 RTLFVDLFSDDLIPKGWDKLADEKPPVDSFSDISIYELHVRDFSISDQSVHPDLRGGYMA 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLGE--VYSLP-----QY 420
           FTLQDSAGI HLKKLSNAG+SHVHLLPTFQF GVDDDKTKWK +    + +LP     Q 
Sbjct: 361 FTLQDSAGINHLKKLSNAGLSHVHLLPTFQFGGVDDDKTKWKFVDTELLENLPPDSAEQQ 420

Query: 421 PLLLQCR--------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+   +        YNPI+WGVPKGSYASD NGPCRL+EFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHGHGPFD NSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMK TMLKAKDAL+ LTKEKNGVDGSSIYIYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGT EVAE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTHEVAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET------- 720
            MLAVSKDHI+ AMAANLRDY+LTNF+G EV G EVLTHD S VAYASCPTET       
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVSAH 720

Query: 721 -------------------------------------GIPFFHCGDELLRSKSMDRDSYN 780
                                                GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNL 840
           SGDWFNRLDFTYMTNNWGVGLP KEKN+ NWPLIKPRLADPSFKPSKSHILAAVEN TNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYI 900
           LQIRYSSPLFRLKT+NAIQKRVRFHN+G SLIPG+IVMSIEDGH GIPGLSQLD  YSYI
Sbjct: 841 LQIRYSSPLFRLKTSNAIQKRVRFHNSGTSLIPGLIVMSIEDGHNGIPGLSQLDSTYSYI 900

Query: 901 VVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPSRTTSVFV 908
           VVVVNARPTE+SFPCPALRA+TLQLHPIQLMSTDP+VKNSTYEPSTGCFMVP RTTSVFV
Sbjct: 901 VVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTYEPSTGCFMVPPRTTSVFV 960

BLAST of Sgr018802 vs. ExPASy TrEMBL
Match: A0A5A7SZ60 (Pullulanase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007110 PE=4 SV=1)

HSP 1 Score: 1592.8 bits (4123), Expect = 0.0e+00
Identity = 794/966 (82.19%), Postives = 832/966 (86.13%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MSLLFSSSLH  PS    TS  Y L PR AA SSSPPA GLFSAA TS S       R G
Sbjct: 1   MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSAAGTSLSHGSHHNFRTG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
           PLY S SSSMSLE+STST+QLEDSLLYSRAFWVS+++IAWNVEV SGSCYLFASKMATLR
Sbjct: 61  PLYCSPSSSMSLEESTSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           VKDGVVEG+DVKI LEKDR  LPENVIKKFPHIQNY PFTVPPASDVE LLKCQLAVATF
Sbjct: 121 VKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           +SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAV+AQI+RDPVGG
Sbjct: 181 DSYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
           MPFEVI LEE+DGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFT+DPY+RG+SSDGR
Sbjct: 241 MPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVDL SDDL P+GWDKLAD+KP V SF+DISIYELH+RD+SISDQSVHPDLRGGYMA
Sbjct: 301 RTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISDQSVHPDLRGGYMA 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLGE--VYSLP-----QY 420
           FTLQDSAGI HLK LSNAG+SHVHLLPTF F GVDDDKTKWK +    + +LP     Q 
Sbjct: 361 FTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTELLENLPPDSAEQQ 420

Query: 421 PLLLQCR--------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+   +        YNPILWGVPKGSYASDPNGPCRL+EFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLH HGP D NSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMK TMLKAKDALQ LTKEKNGVDGSSIYIYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDH HGT EVAE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHHHGTHEVAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET------- 720
            MLAVSKDHI+ AMAANLRDY+LTNF+G EV G EVLTHD S VAYASCPTET       
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVSAH 720

Query: 721 -------------------------------------GIPFFHCGDELLRSKSMDRDSYN 780
                                                GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNL 840
           SGDWFNRLDFTYMTNNWGVGLP KEKN+ NWPLIKPRLADPSFKPSKSHILAAVEN TNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYI 900
           LQIRYSS LFRLKT++AIQKRVRFHN+G SLIPG+IVMSIEDGHEGIPGLSQLD  YSYI
Sbjct: 841 LQIRYSSRLFRLKTSHAIQKRVRFHNSGTSLIPGLIVMSIEDGHEGIPGLSQLDSTYSYI 900

Query: 901 VVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPSRTTSVFV 908
           VVV NARPTE+SFPCPALRA+TLQLHPIQLMSTDPIVKNSTYEPSTGCF+VP RTTSVFV
Sbjct: 901 VVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTYEPSTGCFVVPPRTTSVFV 960

BLAST of Sgr018802 vs. ExPASy TrEMBL
Match: A0A1S3BGM5 (pullulanase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489432 PE=4 SV=1)

HSP 1 Score: 1592.8 bits (4123), Expect = 0.0e+00
Identity = 794/966 (82.19%), Postives = 832/966 (86.13%), Query Frame = 0

Query: 1   MSLLFSSSLHLLPSSTPVTSNSYHLPPRIAAFSSSPPAGGLFSAARTSQSPYPDDYIRGG 60
           MSLLFSSSLH  PS    TS  Y L PR AA SSSPPA GLFSAA TS S       R G
Sbjct: 1   MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSAAGTSLSHGSHHNFRTG 60

Query: 61  PLYYSSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLR 120
           PLY S SSSMSLE+STST+QLEDSLLYSRAFWVS+++IAWNVEV SGSCYLFASKMATLR
Sbjct: 61  PLYCSPSSSMSLEESTSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMATLR 120

Query: 121 VKDGVVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATF 180
           VKDGVVEG+DVKI LEKDR  LPENVIKKFPHIQNY PFTVPPASDVE LLKCQLAVATF
Sbjct: 121 VKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGG 240
           +SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAV+AQI+RDPVGG
Sbjct: 181 DSYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 MPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGR 300
           MPFEVI LEE+DGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFT+DPY+RG+SSDGR
Sbjct: 241 MPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMA 360
           RTLFVDL SDDL P+GWDKLAD+KP V SF+DISIYELH+RD+SISDQSVHPDLRGGYMA
Sbjct: 301 RTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISDQSVHPDLRGGYMA 360

Query: 361 FTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLGE--VYSLP-----QY 420
           FTLQDSAGI HLK LSNAG+SHVHLLPTF F GVDDDKTKWK +    + +LP     Q 
Sbjct: 361 FTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTELLENLPPDSAEQQ 420

Query: 421 PLLLQCR--------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
            L+   +        YNPILWGVPKGSYASDPNGPCRL+EFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLH HGP D NSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMK TMLKAKDALQ LTKEKNGVDGSSIYIYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAE 660
           RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDH HGT EVAE
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHHHGTHEVAE 660

Query: 661 FMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET------- 720
            MLAVSKDHI+ AMAANLRDY+LTNF+G EV G EVLTHD S VAYASCPTET       
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVSAH 720

Query: 721 -------------------------------------GIPFFHCGDELLRSKSMDRDSYN 780
                                                GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNL 840
           SGDWFNRLDFTYMTNNWGVGLP KEKN+ NWPLIKPRLADPSFKPSKSHILAAVEN TNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYI 900
           LQIRYSS LFRLKT++AIQKRVRFHN+G SLIPG+IVMSIEDGHEGIPGLSQLD  YSYI
Sbjct: 841 LQIRYSSRLFRLKTSHAIQKRVRFHNSGTSLIPGLIVMSIEDGHEGIPGLSQLDSTYSYI 900

Query: 901 VVVVNARPTEVSFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPSRTTSVFV 908
           VVV NARPTE+SFPCPALRA+TLQLHPIQLMSTDPIVKNSTYEPSTGCF+VP RTTSVFV
Sbjct: 901 VVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTYEPSTGCFVVPPRTTSVFV 960

BLAST of Sgr018802 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 568/899 (63.18%), Postives = 687/899 (76.42%), Query Frame = 0

Query: 65  SSSSSMSLEDSTSTTQLEDSLLYSRAFWVSKYIIAWNVEVGSGSCYLFASKMATLRVKDG 124
           S+SS MSLE S+  +Q  D L+YSRA+WV++ +IAWNV+VG GSCY +ASK A L   + 
Sbjct: 65  SASSPMSLEVSSPNSQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSED 124

Query: 125 VVEGHDVKIKLEKDRGPLPENVIKKFPHIQNYSPFTVPPASDVETLLKCQLAVATFNSYG 184
            ++G+D++IKLE + G LP +VI+KFPHI+NY  F VP   D+  L+K QLAV  F++ G
Sbjct: 125 GIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEG 184

Query: 185 QCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGGMPFE 244
           +    T LQLPGVLD+LFSY+GPLGA ++ E VSL+LWAPTAQAV   IY++P+   P E
Sbjct: 185 RLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPME 244

Query: 245 VIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTSDPYARGISSDGRRTLF 304
           +  L+E +GVW T+G  SW GCYY Y+V+VYHPST+++E C+ +DPYARG+S+DGR+T  
Sbjct: 245 ICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTFL 304

Query: 305 VDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDYSISDQSVHPDLRGGYMAFTLQ 364
           V+L SDDLKPEGWD LAD+KP + SFSDISIYELH+RD+S +D++V P+ RGGY+AFT +
Sbjct: 305 VNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSK 364

Query: 365 DSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWKSLGE--VYSLPQYPLLLQCR-- 424
           DSAG+ HL+KL +AG++H+HLLPTFQF  VDD+K  WKS+    +  L       Q R  
Sbjct: 365 DSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARIT 424

Query: 425 -----------YNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHL 484
                      YNP+LWGVPKGSYASDP GPCR+IEFRKMVQALN  GL VVLDVVYNHL
Sbjct: 425 EIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHL 484

Query: 485 HGHGPFDLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDY 544
           H  GP D  SVLDKIVPGYYLRRN+DGFIENSTCVNNTASEH+MV+R+I DD+L+WVV+Y
Sbjct: 485 HASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNY 544

Query: 545 KVDGFRFDLMGHLMKRTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVAKNGRGVN 604
           KVDGFRFDLMGH+MK T++ AK A+ SL KE +GVDGS IY+YGEGW+FGEVA+NGRG+N
Sbjct: 545 KVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGIN 604

Query: 605 ASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFMLA 664
           ASQFNL GTGIGSFNDR+RDA LGGSPFGHPLQQGF+TGLLL+PN HDHG++   E ML+
Sbjct: 605 ASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLS 664

Query: 665 VSKDHIQAAMAANLRDYVLTNFKGNEVNGSEVLTHDGSSVAYASCPTET----------- 724
            +K+HIQ  MAANL+DY+LTN +G EV GSEVL HD + VAYAS PTET           
Sbjct: 665 TAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNET 724

Query: 725 ---------------------------------GIPFFHCGDELLRSKSMDRDSYNSGDW 784
                                            GIPFFH GDE+LRSKS+DRDSYNSGDW
Sbjct: 725 LFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDW 784

Query: 785 FNRLDFTYMTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPSKSHILAAVENLTNLLQIR 844
           FNRLDF+Y +NNWGVGLPPK KNE NWPLIKPRL DPSFKP  SHI+A + N  +LL+IR
Sbjct: 785 FNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIR 844

Query: 845 YSSPLFRLKTANAIQKRVRFHNTGASLIPGVIVMSIEDGHEGIPGLSQLDPIYSYIVVVV 904
           YSSPLFRL TA AIQ+RVRFHNTG S IPG IVMSIEDGH GIP +SQ+DPIYS IVV+ 
Sbjct: 845 YSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIF 904

BLAST of Sgr018802 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 123.2 bits (308), Expect = 1.0e-27
Identity = 118/412 (28.64%), Postives = 183/412 (44.42%), Query Frame = 0

Query: 289 DPYA---RGISS--DGRRTLFVDLGSDDLKPEGWDKLADEKPLVHSFSDISIYELHIRDY 348
           DPYA   +G SS  D  +      G+ D +   +D   D K       D+ IYE+++R +
Sbjct: 187 DPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAF 246

Query: 349 SISDQS-VHPDLRGGYMAFTLQDSAGITHLKKLSNAGISHVHLLPTFQFAGVDDDKTKWK 408
           +  + S + P + G Y+ F       I  +  L + GI+ V LLP F+F  ++  +    
Sbjct: 247 TADESSGMDPAIGGSYLGF-------IEKIPHLQDLGINAVELLPVFEFDELELQRR--- 306

Query: 409 SLGEVYSLPQYPLLLQCRYNPILWGVPKGSYASDPNGPCRLI-EFRKMVQALNQIGLRVV 468
                 S P+  ++    Y+ + +  P   YAS    P +   EF++MV+AL+  G+ V+
Sbjct: 307 ------SNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVI 366

Query: 469 LDVVYNHLH---GHGPF--DLNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVER 528
           LDVVYNH +      P+      + +K+   YY+    +  +  S C N     H +V  
Sbjct: 367 LDVVYNHTNEADDKYPYTTSFRGIDNKV---YYMLDPNNQLLNFSGCGNTLNCNHPVVME 426

Query: 529 MIVDDILHWVVDYKVDGFRFDLMGHLMKRT---MLKAKDALQSLTKEKNGVDGSSIYIYG 588
           +I+D + HWV +Y VDGFRFDL   L + T    L A   ++++ K+      S   I  
Sbjct: 427 LILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDS---VLSRCKIIA 486

Query: 589 EGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGL---- 648
           E WD G +   G+  N   ++ +    G + D VR  I G S     ++  F T +    
Sbjct: 487 EPWDCGGLYLVGKFPN---WDRWAEWNGMYRDDVRRFIKGDS----GMKGSFATRVSGSS 546

Query: 649 -LLEPNDHD--HGTDEVAEFMLAVSKDHIQAAMAANLRDYVLTNFKGNEVNG 679
            L + N     HG + V             A     LRD V  NFK NE NG
Sbjct: 547 DLYQVNQRKPYHGVNFVI------------AHDGFTLRDLVSYNFKHNEANG 557