Sgr018664 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr018664
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	RING-type E3 ubiquitin transferase
Location	tig00153207: 251769 .. 252915 (+)
RNA-Seq Expression	Sgr018664
Synteny	Sgr018664

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTCGATGGCGGCGGCGTCTTCCTACTGGTGCTACCGATGCAGTCGCTTTGTTAGGGTTTCGAATCAGGAGTCTGTTGTCTGCCCCGATTGTAATAGTGGGTTCGTCGAACAGATCGAGCATCCGTCGAGGTTTGTGAACGTGGAACCACGCGGAGGAGGTTTCCAGCTGCGGCGATGTACATGATTGGCCACCGCTCGAATTCGGGGCAGAATTTGGGATCTGGGCTTCGGAGATCTCGGAGGAATGGAGGGGATCGCTCTCCTTTCAATCCTGTGATTGTTCTGCGTGGGCCGTCTGATGGGAGTGAAGGTGGAGAAAGTCGTCGGTTTGAGCTTTATTACGACGATGGCGGTGGTTCTGGCTTGAGACCTCTGCCACCGAGCATGTCCGAGTTTTTATTAGGGTCCGGGTTTGATAGGCTTCTGGAGCAACTTTCTCAGATTGAAATGAACGGTATTGGGCGGTTTGAGAACCCGCCGGCATCAAAGGCTGCGATTGAATCAATGCCCACTATTCAAATCTGTGAAAATCATTTGGAAACTGAGTCACACTGCGCTGTTTGCAAAGAGGCATTCGAACTGGGAACCGAAGCACGGGAGATGCCCTGTAAGCATATTTACCATTCTGATTGCATTCTACCTTGGCTTTCGATTCGAAATTCTTGTCCAGTGTGTCGTCATGATTGCCGTCAGATAATCAAAACTCTCCGGATGCTAATGAAAGTGGCGAAAATGGGGGACCAGTGACCTTAAATGGGGAGGAGAACGTGGGCTTAACCATTTGGAGGTTACCGGGCGGTGGGTTTGCGGTAGGGAGGTTTCATGGGGGCAGGAGAGGTGGGGAGCGAGAGCTCCCTGTTGTTTACACTGAGATGGATGGTGGTTTTACCAATGGTGGCGTGCCTAGGAGGGTATCATTTGCTTCAAGAGGCAGAGGGAGAGAGAGAGGCGGGTGGAGCCGTATTATTCGCAATTTGTTTGCTTGCTTCGGGGGATCAATGAGGCCTATGGTACCTACTTCAAGTTCCACTACTGATTCTGGTCCTTCAAGGAGTAGTAGACCACTCTCACATTTCAATACAGAGCCGAGGCGACGGCGCACATGGTCTATGGAGGTTAACAGTGGGACAATCAGCTGGTGA

mRNA sequence

ATGTCGTCGATGGCGGCGGCGTCTTCCTACTGGTGCTACCGATGCAGTCGCTTTGTTAGGTGGGTTCGTCGAACAGATCGAGCATCCGTCGAGGTTTGTGAACGTGGAACCACGCGGAGGAGGTTTCCAGCTGCGGCGATGTACATGATTGGCCACCGCTCGAATTCGGGGCAGAATTTGGGATCTGGGCTTCGGAGATCTCGGAGGAATGGAGGGGATCGCTCTCCTTTCAATCCTGTGATTGTTCTGCGTGGGCCGTCTGATGGGAGTGAAGGTGGAGAAAGTCGTCGGTTTGAGCTTTATTACGACGATGGCGGTGGTTCTGGCTTGAGACCTCTGCCACCGAGCATGTCCGAGTTTTTATTAGGGTCCGGGTTTGATAGGCTTCTGGAGCAACTTTCTCAGATTGAAATGAACGGTATTGGGCGGTTTGAGAACCCGCCGGCATCAAAGGCTGCGATTGAATCAATGCCCACTATTCAAATCTGTGAAAATCATTTGGAAACTGAGTCACACTGCGCTGTTTGCAAAGAGGCATTCGAACTGGGAACCGAAGCACGGGAGATGCCCTATAATCAAAACTCTCCGGATGCTAATGAAAGTGGCGAAAATGGGGGACCAGTGACCTTAAATGGGGAGGAGAACGTGGGCTTAACCATTTGGAGGTTACCGGGCGGTGGGTTTGCGGTAGGGAGGTTTCATGGGGGCAGGAGAGGTGGGGAGCGAGAGCTCCCTGTTGTTTACACTGAGATGGATGGTGGTTTTACCAATGGTGGCGTGCCTAGGAGGGTATCATTTGCTTCAAGAGGCAGAGGGAGAGAGAGAGGCGGGTGGAGCCGTATTATTCGCAATTTGTTTGCTTGCTTCGGGGGATCAATGAGGCCTATGGTACCTACTTCAAGTTCCACTACTGATTCTGGTCCTTCAAGGAGTAGTAGACCACTCTCACATTTCAATACAGAGCCGAGGCGACGGCGCACATGGTCTATGGAGGTTAACAGTGGGACAATCAGCTGGTGA

Coding sequence (CDS)

ATGTCGTCGATGGCGGCGGCGTCTTCCTACTGGTGCTACCGATGCAGTCGCTTTGTTAGGTGGGTTCGTCGAACAGATCGAGCATCCGTCGAGGTTTGTGAACGTGGAACCACGCGGAGGAGGTTTCCAGCTGCGGCGATGTACATGATTGGCCACCGCTCGAATTCGGGGCAGAATTTGGGATCTGGGCTTCGGAGATCTCGGAGGAATGGAGGGGATCGCTCTCCTTTCAATCCTGTGATTGTTCTGCGTGGGCCGTCTGATGGGAGTGAAGGTGGAGAAAGTCGTCGGTTTGAGCTTTATTACGACGATGGCGGTGGTTCTGGCTTGAGACCTCTGCCACCGAGCATGTCCGAGTTTTTATTAGGGTCCGGGTTTGATAGGCTTCTGGAGCAACTTTCTCAGATTGAAATGAACGGTATTGGGCGGTTTGAGAACCCGCCGGCATCAAAGGCTGCGATTGAATCAATGCCCACTATTCAAATCTGTGAAAATCATTTGGAAACTGAGTCACACTGCGCTGTTTGCAAAGAGGCATTCGAACTGGGAACCGAAGCACGGGAGATGCCCTATAATCAAAACTCTCCGGATGCTAATGAAAGTGGCGAAAATGGGGGACCAGTGACCTTAAATGGGGAGGAGAACGTGGGCTTAACCATTTGGAGGTTACCGGGCGGTGGGTTTGCGGTAGGGAGGTTTCATGGGGGCAGGAGAGGTGGGGAGCGAGAGCTCCCTGTTGTTTACACTGAGATGGATGGTGGTTTTACCAATGGTGGCGTGCCTAGGAGGGTATCATTTGCTTCAAGAGGCAGAGGGAGAGAGAGAGGCGGGTGGAGCCGTATTATTCGCAATTTGTTTGCTTGCTTCGGGGGATCAATGAGGCCTATGGTACCTACTTCAAGTTCCACTACTGATTCTGGTCCTTCAAGGAGTAGTAGACCACTCTCACATTTCAATACAGAGCCGAGGCGACGGCGCACATGGTCTATGGAGGTTAACAGTGGGACAATCAGCTGGTGA

Protein sequence

MSSMAAASSYWCYRCSRFVRWVRRTDRASVEVCERGTTRRRFPAAAMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDGGGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICENHLETESHCAVCKEAFELGTEAREMPYNQNSPDANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFTNGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLSHFNTEPRRRRTWSMEVNSGTISW

Homology

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BLAST of Sgr018664 vs. NCBI nr
Match: XP_022138905.1 (probable E3 ubiquitin-protein ligase RHC2A [Momordica charantia])

HSP 1 Score: 594.7 bits (1532), Expect = 4.9e-166
Identity = 309/383 (80.68%), Postives = 316/383 (82.51%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWCYRCSRFVR               +V + +  S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCYRCSRFVRVSNQDSVVCPDCNSGFVEQIEHPSRLVNVEAAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIGHRSNSGQNLGSGLRRSRRNGGDRS FNPVIVLRGPSDGSEGGESRRFELYYDDG
Sbjct: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSQFNPVIVLRGPSDGSEGGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           HLETESHCAVCKEAFELG EAREMP                              NQNSP
Sbjct: 181 HLETESHCAVCKEAFELGNEAREMPCKHIYHSDCILPWLSIRNSCPVCRHELPSDNQNSP 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGENGGPVT NGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENGGPVTSNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGGVPRRVSFASRGRGRERGGWSRI+RNLFACFGGSMR    TSSS TDSGPSRSSRPL 
Sbjct: 301 NGGVPRRVSFASRGRGRERGGWSRILRNLFACFGGSMRSTASTSSSPTDSGPSRSSRPLP 360

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BLAST of Sgr018664 vs. NCBI nr
Match: XP_038890198.1 (probable E3 ubiquitin-protein ligase RHC2A [Benincasa hispida])

HSP 1 Score: 580.9 bits (1496), Expect = 7.3e-162
Identity = 299/383 (78.07%), Postives = 316/383 (82.51%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWCYRCSRFVR               ++ + ++ S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCYRCSRFVRVSNQDSVVCPDCNSGFIEQIEQPSRLVNVEAAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDG+E GESRRFELYYDDG
Sbjct: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGTEAGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTI+IC+N
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIEICDN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           HLETESHCAVCKEAFELG+EAREMP                              NQNS 
Sbjct: 181 HLETESHCAVCKEAFELGSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHELPSDNQNSV 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGENGG VT N +ENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENGGSVTSNWDENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGG+PRRVSFASRGRGRE+GGW RIIRN+FACFGGSMR M  TS+ST+DSGPSR+SRPL 
Sbjct: 301 NGGLPRRVSFASRGRGREQGGWGRIIRNMFACFGGSMRSMASTSNSTSDSGPSRNSRPLP 360

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BLAST of Sgr018664 vs. NCBI nr
Match: XP_008461689.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0050339.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK03553.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 579.7 bits (1493), Expect = 1.6e-161
Identity = 301/383 (78.59%), Postives = 314/383 (81.98%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWCYRCS FVR               +V + ++ S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCYRCSHFVRVSNQDSVVCPDCNSGFVEQIEQPSRLVNVEAAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIG+RSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSE GESRRFELYYDDG
Sbjct: 61  AMYMIGNRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEAGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           HL TESHCAVCKEAFELG EAREMP                              NQNS 
Sbjct: 181 HLATESHCAVCKEAFELGNEAREMPCKHIYHCDCILPWLSLRNSCPVCRHELPSDNQNSL 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGENGG VT NGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENGGSVTSNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGG+PRRVSFASRGRGRERGGW R+IRN+FACFGGSMR +  TS+ST+DSGPSRSSRPL 
Sbjct: 301 NGGLPRRVSFASRGRGRERGGWGRMIRNMFACFGGSMRSIASTSNSTSDSGPSRSSRPLP 360

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BLAST of Sgr018664 vs. NCBI nr
Match: XP_022987501.1 (probable E3 ubiquitin-protein ligase RHC2A [Cucurbita maxima])

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-160
Identity = 299/383 (78.07%), Postives = 312/383 (81.46%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWCYRCSRFVR               ++ + +  S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCYRCSRFVRVFNQDSVVCPDCNSGFIEQIEHPSRLVDVESAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIGHRSNSGQNLGSG+RR RRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG
Sbjct: 61  AMYMIGHRSNSGQNLGSGIRRPRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFE+PPASKAAIESMPTI+ICEN
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFESPPASKAAIESMPTIEICEN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           HLETESHCAVCKEAFELGTEAREMP                              NQNSP
Sbjct: 181 HLETESHCAVCKEAFELGTEAREMPCKHIYHYDCILPWLSIRNSCPVCRHELPSDNQNSP 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGEN G V  NGEENVGLT+WRLPGGGFAVGRF+GGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENEGSVASNGEENVGLTVWRLPGGGFAVGRFNGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGGVPRR+SFASRGRGRERGGWSRIIRN+FACF GSMR M  TS+ST DSG SRSSRPL 
Sbjct: 301 NGGVPRRISFASRGRGRERGGWSRIIRNMFACFRGSMRSMSSTSNSTIDSGSSRSSRPLP 360

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BLAST of Sgr018664 vs. NCBI nr
Match: KAG6600987.1 (putative E3 ubiquitin-protein ligase RHC2A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 574.7 bits (1480), Expect = 5.2e-160
Identity = 299/383 (78.07%), Postives = 311/383 (81.20%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWCYRCSRFVR               ++ + +  S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCYRCSRFVRVFNQDSVVCPDCNSGFIEQIEHPSRLVDVESAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIGHRSNSGQNLGSG+RR RRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG
Sbjct: 61  AMYMIGHRSNSGQNLGSGIRRPRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFE+PPASKAAIESMPTI+ICEN
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFESPPASKAAIESMPTIEICEN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           HLETESHCAVCKEAFELGTEAREMP                              NQNSP
Sbjct: 181 HLETESHCAVCKEAFELGTEAREMPCKHIYHYDCILPWLSIRNSCPVCRHELPSDNQNSP 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGEN G V  NGEENVGLTIWRLPGGGFAVGRF+GGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENEGSVASNGEENVGLTIWRLPGGGFAVGRFNGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGGVPRR+SFASRGRGRERGGWSRIIRN+FACF GSMR M  TS+ST DS  SRSSRPL 
Sbjct: 301 NGGVPRRISFASRGRGRERGGWSRIIRNMFACFRGSMRSMSSTSNSTIDSSSSRSSRPLP 360

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BLAST of Sgr018664 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.6e-58
Identity = 173/405 (42.72%), Postives = 219/405 (54.07%), Query Frame = 0


Query: 6   AASSYWCYRCSRFVRWVRRTDRASVEVCERG---------------------TTRRRFPA 65
           A+ SYWCY CSRFV WV  +D  S   C+ G                     TT+ R P 
Sbjct: 2   ASGSYWCYSCSRFV-WV--SDSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPT 61

Query: 66  AAMYMIGHRSNSGQNLGSG-------LRRSRRNGGDRSPFNPVIVLRG----PSDG--SE 125
                    S    ++ +        + R+R N   RSP NPVIVLRG    PS    SE
Sbjct: 62  RFPPPSSSSSTPSASMHADNSPTPTIVTRTRSN---RSP-NPVIVLRGSAAAPSSDVVSE 121

Query: 126 GGESRRFELYYDDGGGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMN---GIGRFENPP 185
           G +   F++YYDDG  SGLRPLPPSM+EFLLGSGFDRLL+Q+SQIE+N    +   E+PP
Sbjct: 122 GLDRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHPP 181

Query: 186 ASKAAIESMPTIQICENHL--ETESHCAVCKEAFELGTEAREMPYNQ-NSPD-------- 245
           ASK+AIE++P I+I   HL  +++SHCAVCKE F L + AREMP N    PD        
Sbjct: 182 ASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLAI 241

Query: 246 ----------------ANESGENGGPVTLNGEEN----VGLTIWRLPGGGFAVGRFHGGR 305
                            + +G     VT   EE      GLTIWRLPGGGFAVGR  GG 
Sbjct: 242 RNSCPVCRHELPAEDLTDGTGAALTAVTATAEEEEDSAAGLTIWRLPGGGFAVGRIPGGW 301

Query: 306 RGGERELPVVYTEMDGG-FTNGGVPRRVSFASR------GRGRER-GGWSRIIRNLFACF 329
           RGG+R +PVVYTE+DGG   +  +PRRV++ SR      G  RER GG++  I  LF CF
Sbjct: 302 RGGDRMMPVVYTEVDGGRLGDERLPRRVAWGSRRGGRDGGGSRERGGGFAGRIMRLFGCF 361

[Close]

BLAST of Sgr018664 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.9e-54
Identity = 158/365 (43.29%), Postives = 199/365 (54.52%), Query Frame = 0


Query: 8   SSYWCYRCSRFVR-WVRR--TDRASVEV--CERGTTRR--RFPAAAMYMIGHRSNSGQNL 67
           +SYWCY C+RFV  W  +  T   SV    C+ G   +     +AA  +    S   +++
Sbjct: 16  ASYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATELTIPASTEVRSI 75

Query: 68  GSGLRR--SRRNGGDRSPFNPVIVLRGPSDGSEGGES-------RRFELYYDDGGGSGLR 127
            +  R    RR  G R  FNPVIVL+G +   E GE        R FE YYDDG GSGLR
Sbjct: 76  NNNRRSVIRRRRSGRRPSFNPVIVLQGGAGEREEGEEGDAARDRRAFEFYYDDGSGSGLR 135

Query: 128 PLPPSMSEFLLGSGFDRLLEQLSQIEMN--GIGRFENPPASKAAIESMPTIQICENHLET 187
           PLP S+SE L+GSGF+RLLEQLSQIE +  GIGR  NPPASK+AIES+P ++I + H+ +
Sbjct: 136 PLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGS 195

Query: 188 ESHCAVCKEAFELGTEAREMP----YNQNSPDANESGENGGPV------------TLNGE 247
           E++CAVC E FE  TEAREMP    ++ +      S  N  PV            + N E
Sbjct: 196 EANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNE 255

Query: 248 EN--VGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFTNGGV--PRRVSFA-- 307
           E+  VG+TIWRLPGGGFAVGRF+   R GER LPVV TEMDGG        PRR+S+   
Sbjct: 256 EDNAVGMTIWRLPGGGFAVGRFNAAMRDGERVLPVVLTEMDGGGIGNSQDGPRRISWVRS 315

Query: 308 -------SRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLSHFNT 326
                  S G G   G   R +R +       MR + P  SS++ S    +S      N 
Sbjct: 316 HGTLESDSNGTGSGSGSGGR-LRRMVRGMVSLMRRVRPNRSSSSSSAAISTSSDNLDLNI 375

[Close]

BLAST of Sgr018664 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 9.9e-53
Identity = 151/345 (43.77%), Postives = 185/345 (53.62%), Query Frame = 0


Query: 8   SSYWCYRCSRFVR-WVRRTDRASV--EVCERGTTRR-----RFPAAAMYMIGHRSNSGQN 67
           +SYWCY C+RF+  W  +   A V    C  G             AA+        S + 
Sbjct: 29  TSYWCYSCTRFISVWEDQDANAGVLCPYCNGGFIEEIEDSSNSTVAAIPASTPEVRSVEE 88

Query: 68  LGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEG------------GESRRFELYYDDGGG 127
               + R RR    R+ FNPVIVL G   G  G             E R +E YYDDG G
Sbjct: 89  THRSIIR-RRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGATRERRAYEFYYDDGSG 148

Query: 128 SGLRPLPPSMSEFLLGSGFDRLLEQLSQIEM--NGIGRFENPPASKAAIESMPTIQICEN 187
           SGLRPLP S+SE L+GSGF+RLLEQLSQIE   NGIGR  NPPASK+AIES+P ++I + 
Sbjct: 149 SGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDC 208

Query: 188 HLETESHCAVCKEAFELGTEAREMPYN--------------QNSPDANESGENGGPVTLN 247
           H + E++CAVC E FE G E REMP                +NS           P+  +
Sbjct: 209 HTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSDPIQRS 268

Query: 248 GEEN--VGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGG--FTNGGVPRRVSFA 303
            EE   VG+TIWRLPGGGFAVGRF+ G R GER LPVV TEMDGG   +N G PRR+S+ 
Sbjct: 269 NEEEHAVGMTIWRLPGGGFAVGRFNAGVREGERILPVVLTEMDGGGLGSNEG-PRRISWV 328

[Close]

BLAST of Sgr018664 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.4e-11
Identity = 44/150 (29.33%), Postives = 84/150 (56.00%), Query Frame = 0


Query: 42  FPAAAMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELY 101
           FP+++       ++   N  S    +R   GD   F+P   ++   +      ++   + 
Sbjct: 96  FPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLNDLRSSGAQIEFVI 155

Query: 102 YDDGGGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQ 161
            ++    G R LP ++ ++ +G G ++L++QL++ + N   R+  PPASK+AIE++P + 
Sbjct: 156 QNNPSDQGFR-LPANIGDYFIGPGLEQLIQQLAENDPN---RYGTPPASKSAIEALPLVN 215

Query: 162 ICENHLETE-SHCAVCKEAFELGTEAREMP 191
           I +++L +E + CAVC + FE GTEA++MP
Sbjct: 216 ITKSNLNSEFNQCAVCMDDFEEGTEAKQMP 241

[Close]

BLAST of Sgr018664 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 5.5e-11
Identity = 53/166 (31.93%), Postives = 88/166 (53.01%), Query Frame = 0


Query: 32  VCERGTTRRRFPAAAMYM---IGHRSNSGQNLGSGLRRSRRNGGDRSP-FNPVIVLRGPS 91
           V ER  T     A +++M   +  RS+  +  G    R+  +G +  P   P+++  G  
Sbjct: 54  VVERDQTFDLMDAFSVFMRNRLAERSHDREIRG----RTISSGPENFPGLAPLLIFGGQV 113

Query: 92  DGSEGGESRRFELYYDDGGGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENP 151
                G++    L+  +GG  G+     +  ++  G G + L EQLS     G  R   P
Sbjct: 114 PYRLTGDNAVEALF--NGGSPGIGITRGNTGDYFFGPGLEELFEQLSA----GTTRRGPP 173

Query: 152 PASKAAIESMPTIQICENHL-ETESHCAVCKEAFELGTEAREMPYN 193
           PA ++AI+++PTI+I + HL  ++S+C VCK+ FELG+EA++MP N
Sbjct: 174 PAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 209

[Close]

BLAST of Sgr018664 vs. ExPASy TrEMBL
Match: A0A6J1CAT7 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111009972 PE=4 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.4e-166
Identity = 309/383 (80.68%), Postives = 316/383 (82.51%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWCYRCSRFVR               +V + +  S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCYRCSRFVRVSNQDSVVCPDCNSGFVEQIEHPSRLVNVEAAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIGHRSNSGQNLGSGLRRSRRNGGDRS FNPVIVLRGPSDGSEGGESRRFELYYDDG
Sbjct: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSQFNPVIVLRGPSDGSEGGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           HLETESHCAVCKEAFELG EAREMP                              NQNSP
Sbjct: 181 HLETESHCAVCKEAFELGNEAREMPCKHIYHSDCILPWLSIRNSCPVCRHELPSDNQNSP 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGENGGPVT NGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENGGPVTSNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGGVPRRVSFASRGRGRERGGWSRI+RNLFACFGGSMR    TSSS TDSGPSRSSRPL 
Sbjct: 301 NGGVPRRVSFASRGRGRERGGWSRILRNLFACFGGSMRSTASTSSSPTDSGPSRSSRPLP 360

[Close]

BLAST of Sgr018664 vs. ExPASy TrEMBL
Match: A0A5A7UA14 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00480 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.9e-162
Identity = 301/383 (78.59%), Postives = 314/383 (81.98%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWCYRCS FVR               +V + ++ S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCYRCSHFVRVSNQDSVVCPDCNSGFVEQIEQPSRLVNVEAAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIG+RSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSE GESRRFELYYDDG
Sbjct: 61  AMYMIGNRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEAGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           HL TESHCAVCKEAFELG EAREMP                              NQNS 
Sbjct: 181 HLATESHCAVCKEAFELGNEAREMPCKHIYHCDCILPWLSLRNSCPVCRHELPSDNQNSL 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGENGG VT NGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENGGSVTSNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGG+PRRVSFASRGRGRERGGW R+IRN+FACFGGSMR +  TS+ST+DSGPSRSSRPL 
Sbjct: 301 NGGLPRRVSFASRGRGRERGGWGRMIRNMFACFGGSMRSIASTSNSTSDSGPSRSSRPLP 360

[Close]

BLAST of Sgr018664 vs. ExPASy TrEMBL
Match: A0A1S3CGL6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500230 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.9e-162
Identity = 301/383 (78.59%), Postives = 314/383 (81.98%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWCYRCS FVR               +V + ++ S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCYRCSHFVRVSNQDSVVCPDCNSGFVEQIEQPSRLVNVEAAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIG+RSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSE GESRRFELYYDDG
Sbjct: 61  AMYMIGNRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEAGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           HL TESHCAVCKEAFELG EAREMP                              NQNS 
Sbjct: 181 HLATESHCAVCKEAFELGNEAREMPCKHIYHCDCILPWLSLRNSCPVCRHELPSDNQNSL 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGENGG VT NGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENGGSVTSNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGG+PRRVSFASRGRGRERGGW R+IRN+FACFGGSMR +  TS+ST+DSGPSRSSRPL 
Sbjct: 301 NGGLPRRVSFASRGRGRERGGWGRMIRNMFACFGGSMRSIASTSNSTSDSGPSRSSRPLP 360

[Close]

BLAST of Sgr018664 vs. ExPASy TrEMBL
Match: A0A6J1JEE7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485043 PE=4 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.7e-161
Identity = 299/383 (78.07%), Postives = 312/383 (81.46%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWCYRCSRFVR               ++ + +  S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCYRCSRFVRVFNQDSVVCPDCNSGFIEQIEHPSRLVDVESAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIGHRSNSGQNLGSG+RR RRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG
Sbjct: 61  AMYMIGHRSNSGQNLGSGIRRPRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFE+PPASKAAIESMPTI+ICEN
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFESPPASKAAIESMPTIEICEN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           HLETESHCAVCKEAFELGTEAREMP                              NQNSP
Sbjct: 181 HLETESHCAVCKEAFELGTEAREMPCKHIYHYDCILPWLSIRNSCPVCRHELPSDNQNSP 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGEN G V  NGEENVGLT+WRLPGGGFAVGRF+GGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENEGSVASNGEENVGLTVWRLPGGGFAVGRFNGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGGVPRR+SFASRGRGRERGGWSRIIRN+FACF GSMR M  TS+ST DSG SRSSRPL 
Sbjct: 301 NGGVPRRISFASRGRGRERGGWSRIIRNMFACFRGSMRSMSSTSNSTIDSGSSRSSRPLP 360

[Close]

BLAST of Sgr018664 vs. ExPASy TrEMBL
Match: A0A0A0KLW4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G182680 PE=4 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.3e-159
Identity = 298/383 (77.81%), Postives = 313/383 (81.72%), Query Frame = 0


Query: 1   MSSMAAASSYWCYRCSRFVR---------------WVRRTDRASVEVCERGTTRRRFPAA 60
           MSSMAAASSYWC+RCS FVR               +V + +  S  V      RRRFPAA
Sbjct: 1   MSSMAAASSYWCHRCSHFVRVSNQDSVVCPDCNSGFVEQIEHPSRLVNVEAAPRRRFPAA 60

Query: 61  AMYMIGHRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEGGESRRFELYYDDG 120
           AMYMIG+RSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSE GESRRFELYYDDG
Sbjct: 61  AMYMIGNRSNSGQNLGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEAGESRRFELYYDDG 120

Query: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180
           GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN
Sbjct: 121 GGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENPPASKAAIESMPTIQICEN 180

Query: 181 HLETESHCAVCKEAFELGTEAREMPY-----------------------------NQNSP 240
           +L TESHCAVCKEAFELGTEAREMP                              NQNS 
Sbjct: 181 YLATESHCAVCKEAFELGTEAREMPCKHIYHCDCILPWLSIRNSCPVCRHELPSDNQNSL 240

Query: 241 DANESGENGGPVTLNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFT 300
           DA ESGENGG VT NGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTE+DGGFT
Sbjct: 241 DAAESGENGGSVTSNGEENVGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEVDGGFT 300

Query: 301 NGGVPRRVSFASRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLS 340
           NGG+PRRVSFASRGRGRERG W ++IRN+FACFGGSMR +  TS+ST+DSGPSRSSRPL 
Sbjct: 301 NGGLPRRVSFASRGRGRERGRWGQMIRNMFACFGGSMRSIASTSNSTSDSGPSRSSRPLP 360

[Close]

BLAST of Sgr018664 vs. TAIR 10
Match: AT2G39720.1 (RING-H2 finger C2A )

HSP 1 Score: 228.0 bits (580), Expect = 1.1e-59
Identity = 173/405 (42.72%), Postives = 219/405 (54.07%), Query Frame = 0


Query: 6   AASSYWCYRCSRFVRWVRRTDRASVEVCERG---------------------TTRRRFPA 65
           A+ SYWCY CSRFV WV  +D  S   C+ G                     TT+ R P 
Sbjct: 2   ASGSYWCYSCSRFV-WV--SDSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPT 61

Query: 66  AAMYMIGHRSNSGQNLGSG-------LRRSRRNGGDRSPFNPVIVLRG----PSDG--SE 125
                    S    ++ +        + R+R N   RSP NPVIVLRG    PS    SE
Sbjct: 62  RFPPPSSSSSTPSASMHADNSPTPTIVTRTRSN---RSP-NPVIVLRGSAAAPSSDVVSE 121

Query: 126 GGESRRFELYYDDGGGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMN---GIGRFENPP 185
           G +   F++YYDDG  SGLRPLPPSM+EFLLGSGFDRLL+Q+SQIE+N    +   E+PP
Sbjct: 122 GLDRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHPP 181

Query: 186 ASKAAIESMPTIQICENHL--ETESHCAVCKEAFELGTEAREMPYNQ-NSPD-------- 245
           ASK+AIE++P I+I   HL  +++SHCAVCKE F L + AREMP N    PD        
Sbjct: 182 ASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLAI 241

Query: 246 ----------------ANESGENGGPVTLNGEEN----VGLTIWRLPGGGFAVGRFHGGR 305
                            + +G     VT   EE      GLTIWRLPGGGFAVGR  GG 
Sbjct: 242 RNSCPVCRHELPAEDLTDGTGAALTAVTATAEEEEDSAAGLTIWRLPGGGFAVGRIPGGW 301

Query: 306 RGGERELPVVYTEMDGG-FTNGGVPRRVSFASR------GRGRER-GGWSRIIRNLFACF 329
           RGG+R +PVVYTE+DGG   +  +PRRV++ SR      G  RER GG++  I  LF CF
Sbjct: 302 RGGDRMMPVVYTEVDGGRLGDERLPRRVAWGSRRGGRDGGGSRERGGGFAGRIMRLFGCF 361

[Close]

BLAST of Sgr018664 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 212.6 bits (540), Expect = 4.9e-55
Identity = 158/365 (43.29%), Postives = 199/365 (54.52%), Query Frame = 0


Query: 8   SSYWCYRCSRFVR-WVRR--TDRASVEV--CERGTTRR--RFPAAAMYMIGHRSNSGQNL 67
           +SYWCY C+RFV  W  +  T   SV    C+ G   +     +AA  +    S   +++
Sbjct: 16  ASYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATELTIPASTEVRSI 75

Query: 68  GSGLRR--SRRNGGDRSPFNPVIVLRGPSDGSEGGES-------RRFELYYDDGGGSGLR 127
            +  R    RR  G R  FNPVIVL+G +   E GE        R FE YYDDG GSGLR
Sbjct: 76  NNNRRSVIRRRRSGRRPSFNPVIVLQGGAGEREEGEEGDAARDRRAFEFYYDDGSGSGLR 135

Query: 128 PLPPSMSEFLLGSGFDRLLEQLSQIEMN--GIGRFENPPASKAAIESMPTIQICENHLET 187
           PLP S+SE L+GSGF+RLLEQLSQIE +  GIGR  NPPASK+AIES+P ++I + H+ +
Sbjct: 136 PLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGS 195

Query: 188 ESHCAVCKEAFELGTEAREMP----YNQNSPDANESGENGGPV------------TLNGE 247
           E++CAVC E FE  TEAREMP    ++ +      S  N  PV            + N E
Sbjct: 196 EANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNE 255

Query: 248 EN--VGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGGFTNGGV--PRRVSFA-- 307
           E+  VG+TIWRLPGGGFAVGRF+   R GER LPVV TEMDGG        PRR+S+   
Sbjct: 256 EDNAVGMTIWRLPGGGFAVGRFNAAMRDGERVLPVVLTEMDGGGIGNSQDGPRRISWVRS 315

Query: 308 -------SRGRGRERGGWSRIIRNLFACFGGSMRPMVPTSSSTTDSGPSRSSRPLSHFNT 326
                  S G G   G   R +R +       MR + P  SS++ S    +S      N 
Sbjct: 316 HGTLESDSNGTGSGSGSGGR-LRRMVRGMVSLMRRVRPNRSSSSSSAAISTSSDNLDLNI 375

[Close]

BLAST of Sgr018664 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 208.8 bits (530), Expect = 7.0e-54
Identity = 151/345 (43.77%), Postives = 185/345 (53.62%), Query Frame = 0


Query: 8   SSYWCYRCSRFVR-WVRRTDRASV--EVCERGTTRR-----RFPAAAMYMIGHRSNSGQN 67
           +SYWCY C+RF+  W  +   A V    C  G             AA+        S + 
Sbjct: 29  TSYWCYSCTRFISVWEDQDANAGVLCPYCNGGFIEEIEDSSNSTVAAIPASTPEVRSVEE 88

Query: 68  LGSGLRRSRRNGGDRSPFNPVIVLRGPSDGSEG------------GESRRFELYYDDGGG 127
               + R RR    R+ FNPVIVL G   G  G             E R +E YYDDG G
Sbjct: 89  THRSIIR-RRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGATRERRAYEFYYDDGSG 148

Query: 128 SGLRPLPPSMSEFLLGSGFDRLLEQLSQIEM--NGIGRFENPPASKAAIESMPTIQICEN 187
           SGLRPLP S+SE L+GSGF+RLLEQLSQIE   NGIGR  NPPASK+AIES+P ++I + 
Sbjct: 149 SGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDC 208

Query: 188 HLETESHCAVCKEAFELGTEAREMPYN--------------QNSPDANESGENGGPVTLN 247
           H + E++CAVC E FE G E REMP                +NS           P+  +
Sbjct: 209 HTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSDPIQRS 268

Query: 248 GEEN--VGLTIWRLPGGGFAVGRFHGGRRGGERELPVVYTEMDGG--FTNGGVPRRVSFA 303
            EE   VG+TIWRLPGGGFAVGRF+ G R GER LPVV TEMDGG   +N G PRR+S+ 
Sbjct: 269 NEEEHAVGMTIWRLPGGGFAVGRFNAGVREGERILPVVLTEMDGGGLGSNEG-PRRISWV 328

[Close]

BLAST of Sgr018664 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 70.1 bits (170), Expect = 3.9e-12
Identity = 53/166 (31.93%), Postives = 88/166 (53.01%), Query Frame = 0


Query: 32  VCERGTTRRRFPAAAMYM---IGHRSNSGQNLGSGLRRSRRNGGDRSP-FNPVIVLRGPS 91
           V ER  T     A +++M   +  RS+  +  G    R+  +G +  P   P+++  G  
Sbjct: 54  VVERDQTFDLMDAFSVFMRNRLAERSHDREIRG----RTISSGPENFPGLAPLLIFGGQV 113

Query: 92  DGSEGGESRRFELYYDDGGGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENP 151
                G++    L+  +GG  G+     +  ++  G G + L EQLS     G  R   P
Sbjct: 114 PYRLTGDNAVEALF--NGGSPGIGITRGNTGDYFFGPGLEELFEQLSA----GTTRRGPP 173

Query: 152 PASKAAIESMPTIQICENHL-ETESHCAVCKEAFELGTEAREMPYN 193
           PA ++AI+++PTI+I + HL  ++S+C VCK+ FELG+EA++MP N
Sbjct: 174 PAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 209

[Close]

BLAST of Sgr018664 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 70.1 bits (170), Expect = 3.9e-12
Identity = 53/166 (31.93%), Postives = 88/166 (53.01%), Query Frame = 0


Query: 32  VCERGTTRRRFPAAAMYM---IGHRSNSGQNLGSGLRRSRRNGGDRSP-FNPVIVLRGPS 91
           V ER  T     A +++M   +  RS+  +  G    R+  +G +  P   P+++  G  
Sbjct: 54  VVERDQTFDLMDAFSVFMRNRLAERSHDREIRG----RTISSGPENFPGLAPLLIFGGQV 113

Query: 92  DGSEGGESRRFELYYDDGGGSGLRPLPPSMSEFLLGSGFDRLLEQLSQIEMNGIGRFENP 151
                G++    L+  +GG  G+     +  ++  G G + L EQLS     G  R   P
Sbjct: 114 PYRLTGDNAVEALF--NGGSPGIGITRGNTGDYFFGPGLEELFEQLSA----GTTRRGPP 173

Query: 152 PASKAAIESMPTIQICENHL-ETESHCAVCKEAFELGTEAREMPYN 193
           PA ++AI+++PTI+I + HL  ++S+C VCK+ FELG+EA++MP N
Sbjct: 174 PAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 209

The following BLAST results are available for this feature:

BLAST of Sgr018664 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138905.1	4.9e-166	80.68	probable E3 ubiquitin-protein ligase RHC2A [Momordica charantia]	[more]
XP_038890198.1	7.3e-162	78.07	probable E3 ubiquitin-protein ligase RHC2A [Benincasa hispida]	[more]
XP_008461689.1	1.6e-161	78.59	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0050339.1 E...	[more]
XP_022987501.1	1.4e-160	78.07	probable E3 ubiquitin-protein ligase RHC2A [Cucurbita maxima]	[more]
KAG6600987.1	5.2e-160	78.07	putative E3 ubiquitin-protein ligase RHC2A, partial [Cucurbita argyrosperma subs...	[more]

BLAST of Sgr018664 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22283	1.6e-58	42.72	Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q940T5	6.9e-54	43.29	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q9SNB6	9.9e-53	43.77	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
P0CH30	1.4e-11	29.33	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22197	5.5e-11	31.93	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of Sgr018664 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CAT7	2.4e-166	80.68	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11100997...	[more]
A0A5A7UA14	7.9e-162	78.59	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3CGL6	7.9e-162	78.59	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500230 PE=4 ...	[more]
A0A6J1JEE7	6.7e-161	78.07	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485043 P...	[more]
A0A0A0KLW4	1.3e-159	77.81	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G182680 PE...	[more]

BLAST of Sgr018664 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39720.1	1.1e-59	42.72	RING-H2 finger C2A	[more]
AT5G59550.1	4.9e-55	43.29	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G46620.1	7.0e-54	43.77	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G40830.2	3.9e-12	31.93	RING-H2 finger C1A	[more]
AT2G40830.1	3.9e-12	31.93	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010543	Domain of unknown function DUF1117	PFAM	PF06547	DUF1117	coord: 216..311 e-value: 1.5E-34 score: 119.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 293..339
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 56..95
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 295..316
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 203..287 coord: 6..191
None	No IPR available	PANTHER	PTHR15710:SF112	E3 UBIQUITIN-PROTEIN LIGASE RING1-LIKE PROTEIN	coord: 203..287 coord: 6..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr018664.1	Sgr018664.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity