Sgr018626 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr018626 vs. NCBI nr
Match: XP_022146819.1 (SEC14 cytosolic factor [Momordica charantia] >XP_022146820.1 SEC14 cytosolic factor [Momordica charantia])

HSP 1 Score: 422.2 bits (1084), Expect = 3.1e-114
Identity = 210/242 (86.78%), Postives = 228/242 (94.21%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           MENTQQI +AQMRRLVE+ GV TEKYG+PT+MRFLIARSMDPEKAAKMFVQWQKWR+T V
Sbjct: 1   MENTQQIVVAQMRRLVEEHGVPTEKYGEPTMMRFLIARSMDPEKAAKMFVQWQKWRDTTV 60

Query: 61  PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTI 120
           PNG IDESEI+DELEARKIYLQGLSKNGHSVMI+KGNKH+PAKDQVQFKK+VAYSLDK I
Sbjct: 61  PNGRIDESEIKDELEARKIYLQGLSKNGHSVMIVKGNKHYPAKDQVQFKKYVAYSLDKII 120

Query: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFF 180
           +SAF+GREIGNEKLI ILDLQQ+SYKNIDPRGLITGFQSLQAYYPERLA CY LHMPRFF
Sbjct: 121 ASAFQGREIGNEKLIAILDLQQLSYKNIDPRGLITGFQSLQAYYPERLAKCYFLHMPRFF 180

Query: 181 VSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLPQ 240
           VSVWRMVSRFLEKATLEKIVIVS E ER+S+I+EIG EVLPVEYGG+AKL+A+QD VLP 
Sbjct: 181 VSVWRMVSRFLEKATLEKIVIVSCEEERRSIIEEIGEEVLPVEYGGRAKLIALQDVVLPH 240

Query: 241 LD 242
           LD
Sbjct: 241 LD 242

BLAST of Sgr018626 vs. NCBI nr
Match: XP_022943023.1 (CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita moschata] >XP_022943024.1 CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita moschata] >XP_022943025.1 CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita moschata] >KAG6600852.1 hypothetical protein SDJN03_06085, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031486.1 hypothetical protein SDJN02_05526 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 415.6 bits (1067), Expect = 2.9e-112
Identity = 210/243 (86.42%), Postives = 227/243 (93.42%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           MEN+Q+I   QMRR+VEK GVSTEKYGD T+MRFLIARSMD EKAAKMFVQWQKWR+TMV
Sbjct: 1   MENSQRI--LQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMV 60

Query: 61  PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTI 120
           PNG IDESEI+DELEARKIYLQGLS+NGHSVMI+KG+KHFPAKDQVQFKK+V Y+LDKTI
Sbjct: 61  PNGRIDESEIEDELEARKIYLQGLSRNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTI 120

Query: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFF 180
           SSAF+GREIGNEKLIGILDLQQISYKNIDPRGLITGFQ LQAYYPERLA CYIL+MPRFF
Sbjct: 121 SSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF 180

Query: 181 VSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLPQ 240
           +SVWRMVSRFLEK TLEKIVIVSNE ERKSMI+EIG EVLP EYGGK KL+A+QDAVLP 
Sbjct: 181 ISVWRMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPH 240

Query: 241 LDG 243
           LDG
Sbjct: 241 LDG 241

BLAST of Sgr018626 vs. NCBI nr
Match: XP_022989029.1 (CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita maxima] >XP_022989034.1 CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita maxima] >XP_022989036.1 CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita maxima])

HSP 1 Score: 415.6 bits (1067), Expect = 2.9e-112
Identity = 210/243 (86.42%), Postives = 227/243 (93.42%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           MENTQ+I   QMRR+VEK GVSTEKYGD T+MRFLIARSMD EKAAKMFVQWQKWR+TMV
Sbjct: 1   MENTQRI--LQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMV 60

Query: 61  PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTI 120
           PNG IDESEI+DELEARKIYLQGLS+NGHSVMI+KG+KHFPAKDQVQFKK+V Y+LDKTI
Sbjct: 61  PNGRIDESEIEDELEARKIYLQGLSQNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTI 120

Query: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFF 180
           SSAF+GREIGNEKLIGILDLQQISYKNIDPRGLITGFQ LQAYYPERLA CYIL+MPRFF
Sbjct: 121 SSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF 180

Query: 181 VSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLPQ 240
           +SVW+MVSRFLEK TLEKIVIVSNE ERKSMI+EIG EVLP EYGGK KL+A+QDAVLP 
Sbjct: 181 ISVWKMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPH 240

Query: 241 LDG 243
           LDG
Sbjct: 241 LDG 241

BLAST of Sgr018626 vs. NCBI nr
Match: XP_023513848.1 (SEC14 cytosolic factor isoform X1 [Cucurbita pepo subsp. pepo] >XP_023513858.1 SEC14 cytosolic factor isoform X1 [Cucurbita pepo subsp. pepo] >XP_023513865.1 SEC14 cytosolic factor isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 415.2 bits (1066), Expect = 3.8e-112
Identity = 210/243 (86.42%), Postives = 227/243 (93.42%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           MEN+Q+I   QMRR+VEK GVSTEKYGD T+MRFLIARSMD EKAAKMFVQWQKWR+TMV
Sbjct: 1   MENSQRI--LQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMV 60

Query: 61  PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTI 120
           PNG IDESEI+DELEARKIYLQGLS+NGHSVMI+KG+KHFPAKDQVQFKK+V Y+LDKTI
Sbjct: 61  PNGRIDESEIEDELEARKIYLQGLSQNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTI 120

Query: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFF 180
           SSAF+GREIGNEKLIGILDLQQISYKNIDPRGLITGFQ LQAYYPERLA CYIL+MPRFF
Sbjct: 121 SSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF 180

Query: 181 VSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLPQ 240
           +SVWRMVSRFLEK TLEKIVIVSNE ERKSMI+EIG EVLP EYGGK KL+A+QDAVLP 
Sbjct: 181 ISVWRMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPH 240

Query: 241 LDG 243
           LDG
Sbjct: 241 LDG 241

BLAST of Sgr018626 vs. NCBI nr
Match: XP_038893279.1 (CRAL-TRIO domain-containing protein YKL091C-like isoform X1 [Benincasa hispida])

HSP 1 Score: 414.8 bits (1065), Expect = 4.9e-112
Identity = 209/243 (86.01%), Postives = 225/243 (92.59%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           MENTQ+I   QMRRLVEK GVS EKYGDPT+MRFL+ARSMD EKAAKMFVQWQKWR+TMV
Sbjct: 1   MENTQRI--EQMRRLVEKNGVSAEKYGDPTMMRFLVARSMDVEKAAKMFVQWQKWRDTMV 60

Query: 61  PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTI 120
           PNG I ESE++DEL  RK+YLQGLSKNGH+V+ +KGNKHFPAKDQVQFKK+V YSLDKTI
Sbjct: 61  PNGRIVESEVEDELGTRKMYLQGLSKNGHAVLFLKGNKHFPAKDQVQFKKYVVYSLDKTI 120

Query: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFF 180
           SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQ LQAYYPERLA CYIL+MPRFF
Sbjct: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF 180

Query: 181 VSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIGE-VLPVEYGGKAKLVAIQDAVLPQ 240
           VSVWRMVSRFLEKATLEK+VIVSNE ER+SMI+EIGE VLPVEYGGKAKL+AIQD VLP 
Sbjct: 181 VSVWRMVSRFLEKATLEKVVIVSNEEERRSMIEEIGEDVLPVEYGGKAKLIAIQDVVLPH 240

Query: 241 LDG 243
           LDG
Sbjct: 241 LDG 241

BLAST of Sgr018626 vs. ExPASy Swiss-Prot
Match: P24280 (SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC14 PE=1 SV=3)

HSP 1 Score: 86.7 bits (213), Expect = 4.0e-16
Identity = 69/247 (27.94%), Postives = 123/247 (49.80%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWR---- 60
           +++ Q+ ALA++R+L+E  G   E+  D TL+RFL AR  D + A +MF   +KWR    
Sbjct: 29  LDSAQEKALAELRKLLEDAGF-IERLDDSTLLRFLRARKFDVQLAKEMFENCEKWRKDYG 88

Query: 61  -ETMVPNGHIDESEI----------QDELEARKIYLQGL-SKNGHSVMIIKGNKHFPAKD 120
            +T++ + H DE  +          + + + R +Y + L + N H +  +   +      
Sbjct: 89  TDTILQDFHYDEKPLIAKFYPQYYHKTDKDGRPVYFEELGAVNLHEMNKVTSEERMLKNL 148

Query: 121 QVQFKKFVAYSLDKTISSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSL--QA 180
             +++  V Y L     +A  G  +  E    I+DL+ IS  +           S   Q 
Sbjct: 149 VWEYESVVQYRLPACSRAA--GHLV--ETSCTIMDLKGISISSAYSVMSYVREASYISQN 208

Query: 181 YYPERLATCYILHMPRFFVSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIGEVLPVE 230
           YYPER+   YI++ P  F + +R+   FL+  T+ KI I+ +  +++ +     E LPV+
Sbjct: 209 YYPERMGKFYIINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQKELLKQIPAENLPVK 268

BLAST of Sgr018626 vs. ExPASy Swiss-Prot
Match: Q10137 (Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sec14 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 7.6e-15
Identity = 67/250 (26.80%), Postives = 124/250 (49.60%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWR---- 60
           + +TQQ  L  MR  ++KLG  TE+  D TL+RFL AR  + +++ +MF++ +KWR    
Sbjct: 24  LNSTQQATLDSMRLELQKLGY-TERLDDATLLRFLRARKFNLQQSLEMFIKCEKWRKEFG 83

Query: 61  -ETMVPNGHIDESEI----------QDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQ 120
            + ++ N H DE E           + +++ R +Y++ L         I   K +     
Sbjct: 84  VDDLIKNFHYDEKEAVSKYYPQFYHKTDIDGRPVYVEQLGN-------IDLKKLYQITTP 143

Query: 121 VQFKKFVAYSLDKTISSAFK--GREIGN--EKLIGILDLQQISYKNI-DPRGLITGFQSL 180
            +  + + Y  +      F    R+ G   E    I+DL+ +   +I      I    S+
Sbjct: 144 ERMMQNLVYEYEMLALKRFPACSRKAGGLIETSCTIMDLKGVGITSIHSVYSYIRQASSI 203

Query: 181 -QAYYPERLATCYILHMPRFFVSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEI-GEV 229
            Q YYPER+   Y+++ P  F S + ++  FL++AT++KI I+ +   + +++++I  + 
Sbjct: 204 SQDYYPERMGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHILGSNY-KSALLEQIPADN 263

BLAST of Sgr018626 vs. ExPASy Swiss-Prot
Match: P33324 (CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKL091C PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.7e-14
Identity = 72/245 (29.39%), Postives = 115/245 (46.94%), Query Frame = 0

Query: 5   QQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMVPNGH 64
           Q+ AL Q R ++ +     E+  D TL+RFL AR  D   + +MFV+ ++WRE    N  
Sbjct: 29  QEEALLQFRSILLEKNYK-ERLDDSTLLRFLRARKFDINASVEMFVETERWREEYGANTI 88

Query: 65  ID------ESEIQDELEARKIYLQ---GLSKNGHSVMI-------IKGNKHFPAKDQVQF 124
           I+      E+E ++ ++  K+Y Q    + K+G  +         +K       + Q+  
Sbjct: 89  IEDYENNKEAEDKERIKLAKMYPQYYHHVDKDGRPLYFEELGGINLKKMYKITTEKQMLR 148

Query: 125 KKFVAYSLDKTISSAFKGREIGN--EKLIGILDLQQISYKN-IDPRGLITGFQSL-QAYY 184
                Y L  T       R  G   E    +LDL+ IS  N       I     + Q YY
Sbjct: 149 NLVKEYELFATYRVPACSRRAGYLIETSCTVLDLKGISLSNAYHVLSYIKDVADISQNYY 208

Query: 185 PERLATCYILHMPRFFVSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIGEVLPVEYG 230
           PER+   YI+H P  F ++++MV  FL+  T+ KI I+ +  +++ +     E LPV+YG
Sbjct: 209 PERMGKFYIIHSPFGFSTMFKMVKPFLDPVTVSKIFILGSSYKKELLKQIPIENLPVKYG 268

BLAST of Sgr018626 vs. ExPASy Swiss-Prot
Match: P24859 (SEC14 cytosolic factor OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 7.1e-13
Identity = 65/247 (26.32%), Postives = 119/247 (48.18%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWR---- 60
           +++ Q+  L + R L+E LG   E+  D TL+RFL AR  D E +  M+   +KWR    
Sbjct: 27  LDSEQEAKLKEFRELLESLGYK-ERLDDSTLLRFLRARKFDLEASKIMYENCEKWRKEFG 86

Query: 61  -ETMVPNGHIDESEI----------QDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQ 120
            +T+  + H +E  +          + + + R +Y++ L     + M     +    K+ 
Sbjct: 87  VDTIFEDFHYEEKPLVAKYYPQYYHKTDNDGRPVYIEELGSVNLTQMYKITTQERMLKNL 146

Query: 121 V-QFKKFVAYSLDKTISSAFKGREIGNEKLIGILDLQQISYKN-IDPRGLITGFQSL-QA 180
           V +++ FV Y L      A  G  +  E    ILDL+ IS  +       +    ++ Q 
Sbjct: 147 VWEYEAFVRYRLPACSRKA--GYLV--ETSCTILDLKGISISSAAQVLSYVREASNIGQN 206

Query: 181 YYPERLATCYILHMPRFFVSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIGEVLPVE 230
           YYPER+   Y+++ P  F + +R+   FL+  T+ KI I+ +  ++  +     E LP +
Sbjct: 207 YYPERMGKFYLINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQKDLLKQIPAENLPKK 266

BLAST of Sgr018626 vs. ExPASy Swiss-Prot
Match: Q75DK1 (SEC14 cytosolic factor OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 4.6e-12
Identity = 62/248 (25.00%), Postives = 118/248 (47.58%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWR---- 60
           + +  + AL ++R+++++ G  T++  D TL+RFL AR  D   A  MF   +KWR    
Sbjct: 27  LTSEHEAALEELRKVLKQAGF-TKRLDDSTLLRFLRARKFDVAAARAMFENCEKWRKENG 86

Query: 61  -ETMVPNGHIDESEI----------QDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQ 120
            +T+  + H +E  +          + + + R +Y++ L     + M     +    K+ 
Sbjct: 87  VDTIFEDFHYEEKPLVAKFYPQYYHKTDKDGRPVYIEELGAVNLTEMYKITTQERMLKNL 146

Query: 121 V-QFKKFVAYSLDKTISSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGF-----QS 180
           + +++ F  Y L  +   A    +   E    ILDL+ IS   I     +  +       
Sbjct: 147 IWEYESFSRYRLPASSRQA----DCLVETSCTILDLKGIS---ISAAAQVLSYVREASNI 206

Query: 181 LQAYYPERLATCYILHMPRFFVSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIGEVL 228
            Q YYPER+   Y+++ P  F + +R+   FL+  T+ KI I+ +  +++ +     E L
Sbjct: 207 GQNYYPERMGKFYMINAPFGFSAAFRLFKPFLDPVTVSKIFILGSSYQKELLKQIPAENL 266

BLAST of Sgr018626 vs. ExPASy TrEMBL
Match: A0A6J1CYF1 (SEC14 cytosolic factor OS=Momordica charantia OX=3673 GN=LOC111015932 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.5e-114
Identity = 210/242 (86.78%), Postives = 228/242 (94.21%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           MENTQQI +AQMRRLVE+ GV TEKYG+PT+MRFLIARSMDPEKAAKMFVQWQKWR+T V
Sbjct: 1   MENTQQIVVAQMRRLVEEHGVPTEKYGEPTMMRFLIARSMDPEKAAKMFVQWQKWRDTTV 60

Query: 61  PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTI 120
           PNG IDESEI+DELEARKIYLQGLSKNGHSVMI+KGNKH+PAKDQVQFKK+VAYSLDK I
Sbjct: 61  PNGRIDESEIKDELEARKIYLQGLSKNGHSVMIVKGNKHYPAKDQVQFKKYVAYSLDKII 120

Query: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFF 180
           +SAF+GREIGNEKLI ILDLQQ+SYKNIDPRGLITGFQSLQAYYPERLA CY LHMPRFF
Sbjct: 121 ASAFQGREIGNEKLIAILDLQQLSYKNIDPRGLITGFQSLQAYYPERLAKCYFLHMPRFF 180

Query: 181 VSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLPQ 240
           VSVWRMVSRFLEKATLEKIVIVS E ER+S+I+EIG EVLPVEYGG+AKL+A+QD VLP 
Sbjct: 181 VSVWRMVSRFLEKATLEKIVIVSCEEERRSIIEEIGEEVLPVEYGGRAKLIALQDVVLPH 240

Query: 241 LD 242
           LD
Sbjct: 241 LD 242

BLAST of Sgr018626 vs. ExPASy TrEMBL
Match: A0A6J1JN82 (CRAL-TRIO domain-containing protein YKL091C isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486211 PE=4 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.4e-112
Identity = 210/243 (86.42%), Postives = 227/243 (93.42%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           MENTQ+I   QMRR+VEK GVSTEKYGD T+MRFLIARSMD EKAAKMFVQWQKWR+TMV
Sbjct: 1   MENTQRI--LQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMV 60

Query: 61  PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTI 120
           PNG IDESEI+DELEARKIYLQGLS+NGHSVMI+KG+KHFPAKDQVQFKK+V Y+LDKTI
Sbjct: 61  PNGRIDESEIEDELEARKIYLQGLSQNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTI 120

Query: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFF 180
           SSAF+GREIGNEKLIGILDLQQISYKNIDPRGLITGFQ LQAYYPERLA CYIL+MPRFF
Sbjct: 121 SSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF 180

Query: 181 VSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLPQ 240
           +SVW+MVSRFLEK TLEKIVIVSNE ERKSMI+EIG EVLP EYGGK KL+A+QDAVLP 
Sbjct: 181 ISVWKMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPH 240

Query: 241 LDG 243
           LDG
Sbjct: 241 LDG 241

BLAST of Sgr018626 vs. ExPASy TrEMBL
Match: A0A6J1FXA2 (CRAL-TRIO domain-containing protein YKL091C isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447885 PE=4 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.4e-112
Identity = 210/243 (86.42%), Postives = 227/243 (93.42%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           MEN+Q+I   QMRR+VEK GVSTEKYGD T+MRFLIARSMD EKAAKMFVQWQKWR+TMV
Sbjct: 1   MENSQRI--LQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMV 60

Query: 61  PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTI 120
           PNG IDESEI+DELEARKIYLQGLS+NGHSVMI+KG+KHFPAKDQVQFKK+V Y+LDKTI
Sbjct: 61  PNGRIDESEIEDELEARKIYLQGLSRNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTI 120

Query: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFF 180
           SSAF+GREIGNEKLIGILDLQQISYKNIDPRGLITGFQ LQAYYPERLA CYIL+MPRFF
Sbjct: 121 SSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF 180

Query: 181 VSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLPQ 240
           +SVWRMVSRFLEK TLEKIVIVSNE ERKSMI+EIG EVLP EYGGK KL+A+QDAVLP 
Sbjct: 181 ISVWRMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPH 240

Query: 241 LDG 243
           LDG
Sbjct: 241 LDG 241

BLAST of Sgr018626 vs. ExPASy TrEMBL
Match: A0A0A0KIQ9 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G021330 PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.6e-108
Identity = 199/241 (82.57%), Postives = 224/241 (92.95%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           M+NTQ+I   Q+RRLVEK G+STEKYGDP +MRFLIARSMD +KAAKMFVQWQKWR+TMV
Sbjct: 1   MDNTQRI--EQLRRLVEKNGISTEKYGDPMMMRFLIARSMDVDKAAKMFVQWQKWRDTMV 60

Query: 61  PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTI 120
           PNG IDESEI+DEL  RK++LQGLSKNGH+V+ +KG+KHFPAKDQVQFKK+V YSLDKTI
Sbjct: 61  PNGQIDESEIEDELGTRKMFLQGLSKNGHAVLFLKGSKHFPAKDQVQFKKYVVYSLDKTI 120

Query: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFF 180
           SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQ LQAYYPERLA CYIL+MPRFF
Sbjct: 121 SSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQLLQAYYPERLAKCYILNMPRFF 180

Query: 181 VSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIGE-VLPVEYGGKAKLVAIQDAVLPQ 240
           VSVWRMVSRFLEKATLEK+VIV+NE ER+ +++E+GE VLPVE+GGKAKL+AIQD VLPQ
Sbjct: 181 VSVWRMVSRFLEKATLEKVVIVTNEEERRGVVEEVGEDVLPVEFGGKAKLIAIQDVVLPQ 239

BLAST of Sgr018626 vs. ExPASy TrEMBL
Match: A0A6J1FQK5 (SEC14 cytosolic factor isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447885 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.1e-101
Identity = 188/213 (88.26%), Postives = 202/213 (94.84%), Query Frame = 0

Query: 31  LMRFLIARSMDPEKAAKMFVQWQKWRETMVPNGHIDESEIQDELEARKIYLQGLSKNGHS 90
           +MRFLIARSMD EKAAKMFVQWQKWR+TMVPNG IDESEI+DELEARKIYLQGLS+NGHS
Sbjct: 1   MMRFLIARSMDVEKAAKMFVQWQKWRDTMVPNGRIDESEIEDELEARKIYLQGLSRNGHS 60

Query: 91  VMIIKGNKHFPAKDQVQFKKFVAYSLDKTISSAFKGREIGNEKLIGILDLQQISYKNIDP 150
           VMI+KG+KHFPAKDQVQFKK+V Y+LDKTISSAF+GREIGNEKLIGILDLQQISYKNIDP
Sbjct: 61  VMIVKGSKHFPAKDQVQFKKYVVYALDKTISSAFQGREIGNEKLIGILDLQQISYKNIDP 120

Query: 151 RGLITGFQSLQAYYPERLATCYILHMPRFFVSVWRMVSRFLEKATLEKIVIVSNEVERKS 210
           RGLITGFQ LQAYYPERLA CYIL+MPRFF+SVWRMVSRFLEK TLEKIVIVSNE ERKS
Sbjct: 121 RGLITGFQFLQAYYPERLAKCYILNMPRFFISVWRMVSRFLEKVTLEKIVIVSNEEERKS 180

Query: 211 MIDEIG-EVLPVEYGGKAKLVAIQDAVLPQLDG 243
           MI+EIG EVLP EYGGK KL+A+QDAVLP LDG
Sbjct: 181 MIEEIGEEVLPAEYGGKGKLIALQDAVLPHLDG 213

BLAST of Sgr018626 vs. TAIR 10
Match: AT1G14820.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 311.6 bits (797), Expect = 5.5e-85
Identity = 154/241 (63.90%), Postives = 191/241 (79.25%), Query Frame = 0

Query: 1   MENTQQIALAQMRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV 60
           ME +Q++AL Q+R+ VEKL  STE Y  PTLMRFL+ARSMDP KAAKMFV WQKWR +MV
Sbjct: 1   MEESQELALTQLRKSVEKLSSSTEGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMV 60

Query: 61  -PNGHIDESEIQDELEARKIYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKT 120
            P G I ESE+QDELE RK+ LQG +K+GH ++++  +KHF +KD   FKKFV Y+LDKT
Sbjct: 61  PPTGFIPESEVQDELEFRKVCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKT 120

Query: 121 ISSAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRF 180
           I+S   G+E+G EKL+ ++DL  I+YKN+D RGLITGFQ LQ+YYPERLA CYILHMP F
Sbjct: 121 IASGNNGKEVGGEKLVAVIDLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGF 180

Query: 181 FVSVWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLP 240
           FV+VW+ V RFLEKAT EKIVIV++  E++   +EIG + LP EYGG+AKL AIQD +LP
Sbjct: 181 FVTVWKFVCRFLEKATQEKIVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 240

BLAST of Sgr018626 vs. TAIR 10
Match: AT1G14820.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 288.9 bits (738), Expect = 3.8e-78
Identity = 144/222 (64.86%), Postives = 177/222 (79.73%), Query Frame = 0

Query: 21  VSTEK-YGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV-PNGHIDESEIQDELEARK 80
           VS+EK Y  PTLMRFL+ARSMDP KAAKMFV WQKWR +MV P G I ESE+QDELE RK
Sbjct: 7   VSSEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRK 66

Query: 81  IYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTISSAFKGREIGNEKLIGIL 140
           + LQG +K+GH ++++  +KHF +KD   FKKFV Y+LDKTI+S   G+E+G EKL+ ++
Sbjct: 67  VCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVI 126

Query: 141 DLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFFVSVWRMVSRFLEKATLEK 200
           DL  I+YKN+D RGLITGFQ LQ+YYPERLA CYILHMP FFV+VW+ V RFLEKAT EK
Sbjct: 127 DLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEK 186

Query: 201 IVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLPQ 240
           IVIV++  E++   +EIG + LP EYGG+AKL AIQD +LPQ
Sbjct: 187 IVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLPQ 228

BLAST of Sgr018626 vs. TAIR 10
Match: AT1G14820.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 288.9 bits (738), Expect = 3.8e-78
Identity = 144/222 (64.86%), Postives = 177/222 (79.73%), Query Frame = 0

Query: 21  VSTEK-YGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMV-PNGHIDESEIQDELEARK 80
           VS+EK Y  PTLMRFL+ARSMDP KAAKMFV WQKWR +MV P G I ESE+QDELE RK
Sbjct: 7   VSSEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRK 66

Query: 81  IYLQGLSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTISSAFKGREIGNEKLIGIL 140
           + LQG +K+GH ++++  +KHF +KD   FKKFV Y+LDKTI+S   G+E+G EKL+ ++
Sbjct: 67  VCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVI 126

Query: 141 DLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFFVSVWRMVSRFLEKATLEK 200
           DL  I+YKN+D RGLITGFQ LQ+YYPERLA CYILHMP FFV+VW+ V RFLEKAT EK
Sbjct: 127 DLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEK 186

Query: 201 IVIVSNEVERKSMIDEIG-EVLPVEYGGKAKLVAIQDAVLPQ 240
           IVIV++  E++   +EIG + LP EYGG+AKL AIQD +LPQ
Sbjct: 187 IVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLPQ 228

BLAST of Sgr018626 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.2e-39
Identity = 87/225 (38.67%), Postives = 135/225 (60.00%), Query Frame = 0

Query: 12  MRRLVEKLGVSTEKYGDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMVPNGHIDESEIQ 71
           MR L ++    T++  D  + RFL AR +D EKA+ MF+ +  W+ +M+P GHI E+EI 
Sbjct: 34  MRALCDRQDPETKEVDDLMIRRFLRARDLDIEKASTMFLNYLTWKRSMLPKGHIPEAEIA 93

Query: 72  DELEARKIYLQGLSKNGHSVMIIKGNKHFPAK-DQVQFKKFVAYSLDKTISSAFKGREIG 131
           ++L   K+ +QG  K G  + +  GN+H P+K +  +FK+FV Y+L+K  +   +G+   
Sbjct: 94  NDLSHNKMCMQGHDKMGRPIAVAIGNRHNPSKGNPDEFKRFVVYTLEKICARMPRGQ--- 153

Query: 132 NEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFFVSVWRMVSRF 191
            EK + I DLQ   Y N D RG +    +LQ  YPERL   YI+H P  F++ W+++  F
Sbjct: 154 -EKFVAIGDLQGWGYSNCDIRGYLAALSTLQDCYPERLGKLYIVHAPYIFMTAWKVIYPF 213

Query: 192 LEKATLEKIVIVSNEVERKSMIDEIGE-VLPVEYGGKAKLVAIQD 235
           ++  T +KIV V N+    +++++I E  LP  YGGK  LV IQ+
Sbjct: 214 IDANTKKKIVFVENKKLTPTLLEDIDESQLPDIYGGKLPLVPIQE 254

BLAST of Sgr018626 vs. TAIR 10
Match: AT4G08690.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 77.0 bits (188), Expect = 2.3e-14
Identity = 61/226 (26.99%), Postives = 104/226 (46.02%), Query Frame = 0

Query: 5   QQIALAQMRRLVEKLGVSTEKY-GDPTLMRFLIARSMDPEKAAKMFVQWQKWRETMVPNG 64
           +Q  + ++R+L+  L      +  D  ++R+L AR+   +KA KM  +  KWR    P  
Sbjct: 19  EQAKIEEVRKLLGPLPEKLSSFCSDDAVLRYLRARNWHVKKATKMLKETLKWRVQYKPE- 78

Query: 65  HIDESEIQDELEARKIYLQG-LSKNGHSVMIIKGNKHFPAKDQVQFKKFVAYSLDKTISS 124
            I   E+  E E  KIY    + K G  V+I++     P+ +  +  K     L   + +
Sbjct: 79  EICWEEVAGEAETGKIYRSSCVDKLGRPVLIMR-----PSVENSKSVKGQIRYLVYCMEN 138

Query: 125 AFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQSLQAYYPERLATCYILHMPRFFVS 184
           A +    G E+++ ++D    S  N+  R        LQ +YPERLA   + + P+FF  
Sbjct: 139 AVQNLPPGEEQMVWMIDFHGYSLANVSLRTTKETAHVLQEHYPERLAFAVLYNPPKFFEP 198

Query: 185 VWRMVSRFLEKATLEKIVIVSNEVERKSMIDEIG---EVLPVEYGG 226
            W++   FLE  T  K+  V ++     +I E     E + + +GG
Sbjct: 199 FWKVARPFLEPKTRNKVKFVYSDDPNTKVIMEENFDMEKMELAFGG 238