Sgr018564 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr018564 vs. NCBI nr
Match: XP_023544740.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891.0 bits (2301), Expect = 6.6e-255
Identity = 487/735 (66.26%), Postives = 546/735 (74.29%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKMAA       PEQ YTA+IDS+NE+ SLH D++EASNAA+ILYVYKTAISGFAAK
Sbjct: 30  MDTTKMAA-----PTPEQWYTALIDSINEISSLH-DQQEASNAAQILYVYKTAISGFAAK 89

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           LSTKKLHSLSK PGFL+A P ELL                                    
Sbjct: 90  LSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGLQRQHGLWNSSNLASDIVIGLLDT 149

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG C+ G KFS SNCN+KLIGA A++K YEA VGRLN T
Sbjct: 150 GIWPEHISFQDKGLPPVPKKWKGTCQPGQKFSPSNCNRKLIGAMAYIKGYEAIVGRLNAT 209

Query: 181 GSFRSPQDSDGPG-------------------HAQGI-------------DCCWN----- 240
           G+FRSP+DSDG G                    A G+               CW      
Sbjct: 210 GTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCAN 269

Query: 241 ----YAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPH 300
                AIDRAVADGVDV  LSLSL G  S FY DDIAIA FGAV+ GVFVSCSAGN GP 
Sbjct: 270 ADILAAIDRAVADGVDV--LSLSLGGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPS 329

Query: 301 PSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKI-ELPLVYNQSAGRQ 360
            STV N+APWIMTVAASY DR FP +VKLGNG+VFEGSSLY+GN I +LPLVYN +AG Q
Sbjct: 330 SSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEGSSLYTGNNIGQLPLVYNNTAGGQ 389

Query: 361 EANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADA 420
           EAN+CT  SLVPSMVK KIVVCERGTNSRT KGEQVKLAGGAGMILINT+L+GEEL AD 
Sbjct: 390 EANVCTPGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADP 449

Query: 421 HILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEPN 480
           H+LPA +LGASAG+AI  YI+SS+  +PKA I FE TK+G+RAPRVAAFSSRGPS + P+
Sbjct: 450 HVLPAANLGASAGQAIIRYISSSK-HQPKALIGFEGTKFGNRAPRVAAFSSRGPSLIAPD 509

Query: 481 VIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAHK 540
           VIKPDVTAPGVNIL AWP I SPSEL+SDKR VLFN++ GTSMSCPHVS L ALLKSAHK
Sbjct: 510 VIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNVISGTSMSCPHVSGLAALLKSAHK 569

Query: 541 DWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYDI 600
           DWSPAAIKSALMTTAYT DN+MSPISDV SAS  PANPF FGSGHVDP+KA DPGL+YDI
Sbjct: 570 DWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPANPFAFGSGHVDPEKASDPGLIYDI 629

Query: 601 TPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIM-KNARYVTI 648
           TPQDYL+Y CSLNY  +QI L+SRGNFTCPS+R V  QPG+LNYPSFSV M K A+ V++
Sbjct: 630 TPQDYLDYFCSLNYNSTQIGLVSRGNFTCPSKRRVG-QPGNLNYPSFSVFMKKKAKNVSV 689

BLAST of Sgr018564 vs. NCBI nr
Match: XP_022990941.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022990948.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 889.4 bits (2297), Expect = 1.9e-254
Identity = 487/734 (66.35%), Postives = 546/734 (74.39%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKMAA      +PEQ YTA+IDS+N++ SL +D+EEAS+AA+ILYVYKTAISGFAAK
Sbjct: 30  MDTTKMAA-----PSPEQWYTALIDSINKISSL-DDQEEASSAAQILYVYKTAISGFAAK 89

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           LSTKKLHSLSK PGFL+A P ELL                                    
Sbjct: 90  LSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGLQREHGLWNSSNLASDIVIGLLDT 149

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG C+ G KFS SNCN+KLIGA A++K YEA VGRLN T
Sbjct: 150 GIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCNRKLIGATAYIKGYEAIVGRLNAT 209

Query: 181 GSFRSPQDSDGPG-------------------HAQGI-------------DCCWN----- 240
           G+FRSP+DSDG G                    A G+               CW      
Sbjct: 210 GTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCAN 269

Query: 241 ----YAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPH 300
                AIDRAVADGVDV  LSLSL G  S FY DDIAIA FGAV+ GVFVSCSAGN GP 
Sbjct: 270 ADILAAIDRAVADGVDV--LSLSLGGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPS 329

Query: 301 PSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKI-ELPLVYNQSAGRQ 360
            STV N+APWIMTVAASY DR FP +VKLGNG+VFEGSSLYSGN I +LPLVYN +AG +
Sbjct: 330 SSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEGSSLYSGNSIGQLPLVYNNTAGGE 389

Query: 361 EANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADA 420
           EAN+CTA SLVPS+VK KIVVCERGTNSRT KGEQVKLAGGAGMILINT+L+GEEL AD 
Sbjct: 390 EANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADP 449

Query: 421 HILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEPN 480
           H+LPA +LGASAG+AI  YI+SS+  +PKA I FE TK+G+RAPRVAAFSSRGPS + P+
Sbjct: 450 HVLPAANLGASAGQAIIRYISSSK-HQPKALIAFEGTKFGNRAPRVAAFSSRGPSLIAPD 509

Query: 481 VIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAHK 540
           VIKPDVTAPGVNIL AWP I SPSEL+SDKR VLFNI+ GTSMSCPHVS L ALLKSAHK
Sbjct: 510 VIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSAHK 569

Query: 541 DWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYDI 600
           DWSPAAIKSALMTTAYT DN+MSPISDV S S  PANPF FGSGHVDP+KA DPGL+YDI
Sbjct: 570 DWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPANPFAFGSGHVDPEKASDPGLIYDI 629

Query: 601 TPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIM-KNARYVTI 647
           TPQDYLNY CSLNY  +QI L+SRGNFTCPS+R V  QPG+LNYPSFSV M K A+ V++
Sbjct: 630 TPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVG-QPGNLNYPSFSVFMKKKAKNVSV 689

BLAST of Sgr018564 vs. NCBI nr
Match: XP_022941552.1 (subtilisin-like protease SBT1.1 [Cucurbita moschata] >XP_022941553.1 subtilisin-like protease SBT1.1 [Cucurbita moschata])

HSP 1 Score: 887.1 bits (2291), Expect = 9.5e-254
Identity = 487/735 (66.26%), Postives = 545/735 (74.15%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKMAA       PEQ YTA+IDS+NE+ SL ED+EEASNAA+ILYVYKTAISGFAAK
Sbjct: 30  MDTTKMAA-----PTPEQWYTALIDSINEISSL-EDQEEASNAAQILYVYKTAISGFAAK 89

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           LSTKKLHSLSK PGFL+A P ELL                                    
Sbjct: 90  LSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGLQREHGLWNSSNLASDIVIGLLDT 149

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG C+ G KFS SNCN+KLIGA A++K YEA VGRLN T
Sbjct: 150 GIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCNRKLIGAIAYIKGYEAIVGRLNAT 209

Query: 181 GSFRSPQDSDGPG-------------------HAQGI-------------DCCWN----- 240
           G+FRSP+DSDG G                    A G+               CW      
Sbjct: 210 GTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCAN 269

Query: 241 ----YAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPH 300
                AIDRAVADGVDV  LSLSL G  S FY DDIAIA FGAV+ GVFVSCSAGN GP 
Sbjct: 270 ADILAAIDRAVADGVDV--LSLSLGGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPS 329

Query: 301 PSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKI-ELPLVYNQSAGRQ 360
            STV N+APWIMTVAASY DR FP +VKLGNG+VFEGSSLYSGN I +LPLVYN +AG +
Sbjct: 330 SSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFEGSSLYSGNNIGQLPLVYNNTAGGE 389

Query: 361 EANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADA 420
           +AN+CTA SLVPSMVK KIVVCERGTNSRT KGEQVKLAGGAGMILINT+L+GEEL AD 
Sbjct: 390 DANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADP 449

Query: 421 HILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEPN 480
           H+LPA +LGASAG+AI  YI+SS+  +PKA I FE TK+G+RAPRVAAFSSRGPS + P+
Sbjct: 450 HVLPAANLGASAGQAIIRYISSSK-HQPKALIAFEGTKFGNRAPRVAAFSSRGPSLIAPD 509

Query: 481 VIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAHK 540
           VIKPDVTAPGVNIL AWP I SPSE++SDKR VLFN++ GTSMSCPHVS L ALLKSAHK
Sbjct: 510 VIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNVISGTSMSCPHVSGLAALLKSAHK 569

Query: 541 DWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYDI 600
           DWSPAAIKSALMTTAYT DN+MSPISDV SAS  PANPF FGSGHVDP+KA DPGL+YDI
Sbjct: 570 DWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPANPFAFGSGHVDPEKASDPGLIYDI 629

Query: 601 TPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIM-KNARYVTI 648
           TPQDYLNYLCSLNY  +QI L+SRGNFTCPS+R V  Q G LNYPSFSV M K A+ V++
Sbjct: 630 TPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVG-QAGKLNYPSFSVFMKKKAKNVSV 689

BLAST of Sgr018564 vs. NCBI nr
Match: XP_022989707.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022989715.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 884.4 bits (2284), Expect = 6.2e-253
Identity = 476/734 (64.85%), Postives = 548/734 (74.66%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKMA       NPEQ YT++I S+N+L S+++D+ EASNAAEILY+YKTAISGF+AK
Sbjct: 31  MDATKMAT-----TNPEQWYTSIIHSINQLSSINDDQNEASNAAEILYIYKTAISGFSAK 90

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           LST+KLHSLSK+PGFL+A P++LL                                    
Sbjct: 91  LSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQFLGLQRGHGLWNASNLASDIIIGVIDT 150

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG C+ GPKFSRSNCNKKL+GARA++K YE  +GRLN T
Sbjct: 151 GIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSRSNCNKKLVGARAYIKGYETILGRLNTT 210

Query: 181 GSFRSPQDSDGPG-----------------HAQGIDC---------------CWN----- 240
           G+FRS +DSDG G                 + QG+                 CW      
Sbjct: 211 GTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMGAATGMRFTSRIAAYKVCWPEGCAS 270

Query: 241 ----YAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPH 300
                AIDRAV DGVDV  LSLSL G    FY D+IAIAAFGAV+ GVFVSCSAGN GP 
Sbjct: 271 TDILAAIDRAVVDGVDV--LSLSLGGGDGFFYQDEIAIAAFGAVRNGVFVSCSAGNSGPF 330

Query: 301 PSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKI-ELPLVYNQSAGRQ 360
            STV NVAPWIMTVAASY DR FP SVKLGNG++FEGSSL+SGN I ELPLVYN++AG +
Sbjct: 331 MSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIFEGSSLHSGNSIGELPLVYNKTAGGE 390

Query: 361 EANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADA 420
           EA++CTA SLVPSMVK KIVVCERGTNSR EKGEQVKLAGG GMILINT+L+GEEL  D+
Sbjct: 391 EASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVKLAGGVGMILINTQLEGEELFGDS 450

Query: 421 HILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEPN 480
           H+LPA +LGASAGKAI +YI SS+   PKASI+FE T+YGSRAPR+AAFSSRGPS  EP 
Sbjct: 451 HVLPAINLGASAGKAIINYIASSK-HPPKASILFEGTRYGSRAPRMAAFSSRGPSFFEPY 510

Query: 481 VIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAHK 540
           VIKPD+TAPGVNIL AWPP++SPSELKSDKR VLFNI+ GTSMSCPHVS + ALLKSAHK
Sbjct: 511 VIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNIISGTSMSCPHVSGIAALLKSAHK 570

Query: 541 DWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYDI 600
           +WSPAAIKSALMTTAY  DNK S ISDV   S  PA+P+ FGSGHVDP+KA DPGLVYDI
Sbjct: 571 NWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGPADPYAFGSGHVDPEKAVDPGLVYDI 630

Query: 601 TPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIMKNARYVTIT 648
            PQDYLNYLCSLNYT  Q+ L+SRGNF+CPS+R V LQPGDLNYPSFSV MKN +   + 
Sbjct: 631 VPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTV-LQPGDLNYPSFSVSMKN-KAKNVR 690

BLAST of Sgr018564 vs. NCBI nr
Match: XP_022990928.1 (subtilisin-like protease SBT1.1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 879.4 bits (2271), Expect = 2.0e-251
Identity = 482/734 (65.67%), Postives = 546/734 (74.39%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKM A      +PEQ Y+AMI SVN++ SLH+DEEEASNAAEILYVYKTAISGFAAK
Sbjct: 31  MDTTKMVA----TTSPEQWYSAMIHSVNKISSLHQDEEEASNAAEILYVYKTAISGFAAK 90

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           L+TKKLHSLSK+PGFL+A P+ELL                                    
Sbjct: 91  LTTKKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLHREHGLWNSSNSASDIIIGLIDS 150

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG CE GPKFS SNCNKKL+GARA++  YEA VGRLN T
Sbjct: 151 GIWPEHISFQDKGLPPVPKKWKGACEAGPKFSPSNCNKKLVGARAYIDGYEAIVGRLNVT 210

Query: 181 GSFRSPQDSDGPG-----------------HAQG---------------IDCCWNY---- 240
           G+FRSP+DS+G G                 ++QG                  CW      
Sbjct: 211 GTFRSPRDSNGHGTHTASTAAGNIVNKASLYSQGKGVATGISFTSRIAAYKACWPMGCAN 270

Query: 241 -----AIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGP- 300
                A+DRAVADGVD+  LSLSL G  + FY D+IAIAAFGAVQ GVFVSCSAGN+G  
Sbjct: 271 ADILAAMDRAVADGVDI--LSLSLGGGVTAFYKDNIAIAAFGAVQNGVFVSCSAGNFGSL 330

Query: 301 HPSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSG-NKIELPLVYNQSAGR 360
             S V N APWIMTVAASY DR FPT+VKLGNG+VFEGSSLYSG N  ELPLVYN + G 
Sbjct: 331 FRSAVSNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYSGQNTAELPLVYNNTVGG 390

Query: 361 QEANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVAD 420
           QEANLCT  SLVP+MVK KIVVC RG N R  KGEQVKLAGGAGMILINT+LDGEEL A+
Sbjct: 391 QEANLCTPGSLVPAMVKGKIVVCGRGKNPRVAKGEQVKLAGGAGMILINTQLDGEELFAE 450

Query: 421 AHILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEP 480
            H+LPATSLGASA KA+  YI SS+ ++P ASI F+ TKYGSRAP+VAAFSSRGPS + P
Sbjct: 451 PHVLPATSLGASATKAMLDYIASSK-NQPTASIGFQGTKYGSRAPKVAAFSSRGPSLIGP 510

Query: 481 NVIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAH 540
           +VIKPDVTAPGVNIL AWPPI+SPSEL+ DKR VLFNI+ GTSMSCPHVS L AL+KSAH
Sbjct: 511 DVIKPDVTAPGVNILAAWPPIVSPSELEFDKRRVLFNIISGTSMSCPHVSGLAALIKSAH 570

Query: 541 KDWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYD 600
            +WSPAAIKSALMTTAYTTDNKMSPISDVS  +  PA+PF  GSGHVDP+KA DPGLVYD
Sbjct: 571 NNWSPAAIKSALMTTAYTTDNKMSPISDVSHPNGEPADPFAIGSGHVDPEKASDPGLVYD 630

Query: 601 ITPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIMKNARYVTI 647
           I P DYLNYLCSLNY+ +QI LLS+GNF+CPS+R V ++PGDLNYPSFSVIMK A+ V++
Sbjct: 631 IAPGDYLNYLCSLNYSSTQIGLLSKGNFSCPSKREV-VEPGDLNYPSFSVIMK-AKNVSV 690

BLAST of Sgr018564 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 7.8e-166
Identity = 334/714 (46.78%), Postives = 431/714 (60.36%), Query Frame = 0

Query: 23  MIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAKLSTKKLHSLSKMPGFLSANPEE 82
           ++ S+   L      ++  +  EI Y+Y+ A+SGF+A L+  +L ++    GF+SA P+E
Sbjct: 56  IVTSLFNSLQTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDE 115

Query: 83  LLQ---------------------------------DEGLPPE------------PARWK 142
           LL                                  D G+ PE            P+RW+
Sbjct: 116 LLSLHTTYSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWR 175

Query: 143 GVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNETGSFRSPQDSDGP---------- 202
           G C+EG  FS S CNKK+IGA AF K YE+ VG++NET  FRS +D+ G           
Sbjct: 176 GSCDEGTNFSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAG 235

Query: 203 ---------GHAQGI-------------DCCWNY---------AIDRAVADGVDVLSLSL 262
                    G A+G+               CW           AIDRA+ DGVDV  +SL
Sbjct: 236 DIVPKANYFGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDV--ISL 295

Query: 263 SLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPHPSTVDNVAPWIMTVAASYIDRI 322
           SL G + PFY D IAIA FGA+QK +FVSCSAGN GP  STV N APW+MTVAASY DR 
Sbjct: 296 SLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRT 355

Query: 323 FPTSVKLGNGEVFEGSSLYSGNKIE-LPLVYNQSAGRQE-ANLCTASSLVPSMVKRKIVV 382
           FP  V++GN +   GSSLY G  ++ LPL +N++AG +  A  C   SL   +V+ KIV+
Sbjct: 356 FPAIVRIGNRKSLVGSSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVI 415

Query: 383 CERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADAHILPATSLGASAGKAIKSYIT 442
           C RG + RT KGE+VK +GGA M+L++TE +GEEL+AD H+LPA SLG S GK + +Y+ 
Sbjct: 416 CLRGASGRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLA 475

Query: 443 SSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEPNVIKPDVTAPGVNILVAWPPII 502
            + ++   AS+ F  T YG+ AP VAAFSSRGPS   P + KPD+ APG+NIL  W P  
Sbjct: 476 GAANA--TASVRFRGTAYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFS 535

Query: 503 SPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAHKDWSPAAIKSALMTTAYTTDNK 562
           SPS L+SD R V FNI+ GTSM+CPH+S + AL+KS H DWSPA IKSA+MTTA  TDN+
Sbjct: 536 SPSLLRSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNR 595

Query: 563 MSPISDVSSA-SFVPANPFTFGSGHVDPKKAFDPGLVYDITPQDYLNYLCSLNYTPSQIA 622
             PI D  +A +   A  F FG+G+VDP +A DPGLVYD +  DYLNYLCSLNYT  +I 
Sbjct: 596 NRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERIL 655

Query: 623 LLSRGNFTCPSRRIVFLQPGDLNYPSFSV-IMKNARYVTITFKRTVTNVGSPSSVYTVQI 647
           L S  N+TC S  +V L PGDLNYPSF+V ++  A   T+ +KRTVTNVGSP+  Y V +
Sbjct: 656 LFSGTNYTCASNAVV-LSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHV 715

BLAST of Sgr018564 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 4.0e-146
Identity = 301/617 (48.78%), Postives = 378/617 (61.26%), Query Frame = 0

Query: 86  DEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNETGSFRSPQDSD 145
           D GL P P +WKG C     F  S CN+KL+GAR F   YEA  G++NET  FRSP+DSD
Sbjct: 149 DRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSD 208

Query: 146 GP-------------------GHAQGI-------------DCCWN---------YAIDRA 205
           G                    G+A G+               CWN          A D A
Sbjct: 209 GHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTA 268

Query: 206 VADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPHPSTVDNVAPW 265
           VADGVDV  +SLS+ G   P+Y D IAI AFGA+ +G+FVS SAGN GP   TV NVAPW
Sbjct: 269 VADGVDV--ISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPW 328

Query: 266 IMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKIE----LPLVYNQS---AGRQEANL 325
           + TV A  IDR FP +VKLGNG++  G S+Y G  ++     PLVY  S        ++L
Sbjct: 329 MTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSL 388

Query: 326 CTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADAHILP 385
           C   SL P++VK KIV+C+RG NSR  KGE V+  GG GMI+ N   DGE LVAD H+LP
Sbjct: 389 CLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLP 448

Query: 386 ATSLGASAGKAIKSYITSSRDSK----PKASIVFEQTKYGSR-APRVAAFSSRGPSSVEP 445
           ATS+GAS G  I+ YI+ S  S+    P A+IVF+ T+ G R AP VA+FS+RGP+   P
Sbjct: 449 ATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETP 508

Query: 446 NVIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAH 505
            ++KPDV APG+NIL AWP  I PS + SD R   FNI+ GTSM+CPHVS L ALLK+AH
Sbjct: 509 EILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAH 568

Query: 506 KDWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYD 565
            DWSPAAI+SAL+TTAYT DN   P+ D S+ +   ++   +GSGHV P KA DPGLVYD
Sbjct: 569 PDWSPAAIRSALITTAYTVDNSGEPMMDESTGN--TSSVMDYGSGHVHPTKAMDPGLVYD 628

Query: 566 ITPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIMK--NARYV 625
           IT  DY+N+LC+ NYT + I  ++R    C   R      G+LNYPSFSV+ +      +
Sbjct: 629 ITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAG-HVGNLNYPSFSVVFQQYGESKM 688

Query: 626 TITFKRTVTNVGSPSSVYTVQINNPKGVAITVKPKKLSFVRLGEKLSYQVSFVALEKKEA 646
           +  F RTVTNVG   SVY ++I  P+G  +TV+P+KLSF R+G+KLS+ V     E K +
Sbjct: 689 STHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLS 748

BLAST of Sgr018564 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.1e-143
Identity = 304/700 (43.43%), Postives = 403/700 (57.57%), Query Frame = 0

Query: 41  SNAAEILYVYKTAISGFAAKLSTKKLHSLSKMPGFLSANPEELLQ--------------- 100
           S++AE+LY Y+ AI GF+ +L+ ++  SL   PG +S  PE   +               
Sbjct: 60  SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 119

Query: 101 -------------------------------DEGLPPEPARWKGVCEEGPKFSRSNCNKK 160
                                          DEG  P P+ WKG CE G  F+ S CN+K
Sbjct: 120 TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRK 179

Query: 161 LIGARAFLKAYEAGVGRLNETGSFRSPQDSDGP-------------------GHAQG--- 220
           LIGAR F + YE+ +G ++E+   RSP+D DG                    G+A G   
Sbjct: 180 LIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 239

Query: 221 ----------IDCCW---------NYAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIA 280
                        CW           AID+A+AD V+V  LS+SL G  S +Y D +AI 
Sbjct: 240 GMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNV--LSMSLGGGMSDYYRDGVAIG 299

Query: 281 AFGAVQKGVFVSCSAGNYGPHPSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSS 340
           AF A+++G+ VSCSAGN GP  S++ NVAPWI TV A  +DR FP    LGNG+ F G S
Sbjct: 300 AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 359

Query: 341 LYSGNKIE---LPLVY-NQSAGRQEANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQV 400
           L+ G  +    LP +Y   ++     NLC   +L+P  VK KIV+C+RG N+R +KG+ V
Sbjct: 360 LFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVV 419

Query: 401 KLAGGAGMILINTELDGEELVADAHILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQ 460
           K AGG GMIL NT  +GEELVADAH+LPAT++G  AG  I+ Y+T+  D  P ASI    
Sbjct: 420 KAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTT--DPNPTASISILG 479

Query: 461 TKYGSR-APRVAAFSSRGPSSVEPNVIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLF 520
           T  G + +P VAAFSSRGP+S+ PN++KPD+ APGVNIL AW     P+ L SD R V F
Sbjct: 480 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 539

Query: 521 NIVLGTSMSCPHVSSLVALLKSAHKDWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVP 580
           NI+ GTSMSCPHVS L ALLKS H +WSPAAI+SALMTTAY T     P+ D+++    P
Sbjct: 540 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK--P 599

Query: 581 ANPFTFGSGHVDPKKAFDPGLVYDITPQDYLNYLCSLNYTPSQIALLSRGNFTC-PSRRI 640
           + PF  G+GHV P  A +PGL+YD+T +DYL +LC+LNYT  QI  +SR N+TC PS+  
Sbjct: 600 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS- 659

Query: 641 VFLQPGDLNYPSFSVIMKNARYVTITFKRTVTNVGSPSSVYTVQI-NNPKGVAITVKPKK 647
                 DLNYPSF+V +         + RTVT+VG  +  Y+V++ +   GV I+V+P  
Sbjct: 660 --YSVADLNYPSFAVNVDGVG--AYKYTRTVTSVGG-AGTYSVKVTSETTGVKISVEPAV 719

BLAST of Sgr018564 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 4.6e-142
Identity = 305/696 (43.82%), Postives = 396/696 (56.90%), Query Frame = 0

Query: 39  EASNAAEILYVYKTAISGFAAKLSTKKLHSL--------------------SKMPGFLSA 98
           + ++ + +LY Y T+  GF+A L + +  SL                    ++ P FL  
Sbjct: 53  QLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL 112

Query: 99  NPEELLQDEG--------------------------LPPEPARWKGVCEEGPKFSRSNCN 158
           N E  + D G                          +P  P++WKG CE G  F    CN
Sbjct: 113 NSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCN 172

Query: 159 KKLIGARAFLKAYE-AGVGRLNETGSFRSPQDSDG-----------------------PG 218
           KKLIGAR+F K ++ A  G  +      SP+D DG                        G
Sbjct: 173 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 232

Query: 219 HAQGI---------DCCWN---------YAIDRAVADGVDVLSLSLSLNGDTSPFYSDDI 278
            A+G+           CW+          A+DRA+ DGVDV  LSLSL G ++P+Y D I
Sbjct: 233 TARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDV--LSLSLGGGSAPYYRDTI 292

Query: 279 AIAAFGAVQKGVFVSCSAGNYGPHPSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFE 338
           AI AF A+++GVFVSCSAGN GP  ++V NVAPW+MTV A  +DR FP    LGNG+   
Sbjct: 293 AIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLT 352

Query: 339 GSSLYSG---NKIELPLVYNQSAGRQEANLCTASSLVPSMVKRKIVVCERGTNSRTEKGE 398
           G SLYSG       L LVYN+      +NLC   SL  S+V+ KIVVC+RG N+R EKG 
Sbjct: 353 GVSLYSGVGMGTKPLELVYNK-GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGA 412

Query: 399 QVKLAGGAGMILINTELDGEELVADAHILPATSLGASAGKAIKSYITSSRDSKPKASIVF 458
            V+ AGG GMI+ NT   GEELVAD+H+LPA ++G   G  ++ Y+ S  DSKP A +VF
Sbjct: 413 VVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKS--DSKPTALLVF 472

Query: 459 EQTKYGSR-APRVAAFSSRGPSSVEPNVIKPDVTAPGVNILVAWPPIISPSELKSDKRSV 518
           + T    + +P VAAFSSRGP++V P ++KPDV  PGVNIL  W   I P+ L  D R  
Sbjct: 473 KGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRT 532

Query: 519 LFNIVLGTSMSCPHVSSLVALLKSAHKDWSPAAIKSALMTTAYTTDNKMSPISDVSSASF 578
            FNI+ GTSMSCPH+S L  LLK+AH +WSP+AIKSALMTTAY  DN  +P+ D +  S 
Sbjct: 533 QFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSL 592

Query: 579 VPANPFTFGSGHVDPKKAFDPGLVYDITPQDYLNYLCSLNYTPSQI-ALLSRGNFTCPSR 638
             +NP+  GSGHVDP+KA  PGLVYDI+ ++Y+ +LCSL+YT   I A++ R +  C  +
Sbjct: 593 --SNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK 652

Query: 639 RIVFLQPGDLNYPSFSVIMKNARYVTITFKRTVTNVGSPSSVYTVQINNPKGVAITVKPK 642
              F  PG LNYPSFSV+    R V  T  R VTNVG+ SSVY V +N    V I+VKP 
Sbjct: 653 ---FSDPGQLNYPSFSVLFGGKRVVRYT--REVTNVGAASSVYKVTVNGAPSVGISVKPS 712

BLAST of Sgr018564 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 2.3e-141
Identity = 303/741 (40.89%), Postives = 417/741 (56.28%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MDK+ M        N  Q Y++ I+SV +    H+ +EE  N   ILY Y+TA  G AA+
Sbjct: 41  MDKSAMPL---PYTNHLQWYSSKINSVTQ----HKSQEEEGNNNRILYTYQTAFHGLAAQ 100

Query: 61  LSTKKLHSLSKMPGFLSANPE--------------------------------------- 120
           L+ ++   L +  G ++  PE                                       
Sbjct: 101 LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 160

Query: 121 --------ELLQDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLN 180
                   E   D G+ P PA W+G CE G +F + NCN+K++GAR F + YEA  G+++
Sbjct: 161 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 220

Query: 181 ETGSFRSPQDSDGP-----------------------GHAQGI---------DCCW---- 240
           E   ++SP+D DG                        G A+G+           CW    
Sbjct: 221 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 280

Query: 241 -----NYAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYG 300
                  A+D+AVADGV V  LS+SL G  S +  D ++IA FGA++ GVFVSCSAGN G
Sbjct: 281 FSSDILSAVDQAVADGVQV--LSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGG 340

Query: 301 PHPSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKI-----ELPLVY- 360
           P P ++ NV+PWI TV AS +DR FP +VK+G    F+G SLY G  +     + PLVY 
Sbjct: 341 PDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYL 400

Query: 361 -NQSAGRQEANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELD 420
              ++     + C   +L    V  KIV+C+RG   R +KG+ VK AGG GM+L NT  +
Sbjct: 401 GRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATN 460

Query: 421 GEELVADAHILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSR-APRVAAFSS 480
           GEELVAD+H+LPA ++G   GK IK Y  +S+  K  AS+    T+ G + +P VAAFSS
Sbjct: 461 GEELVADSHMLPAVAVGEKEGKLIKQYAMTSK--KATASLEILGTRIGIKPSPVVAAFSS 520

Query: 481 RGPSSVEPNVIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSL 540
           RGP+ +   ++KPD+ APGVNIL AW   ++PS L SD R V FNI+ GTSMSCPHVS +
Sbjct: 521 RGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGV 580

Query: 541 VALLKSAHKDWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKA 600
            AL+KS H DWSPAAIKSALMTTAY  DN   P++D S A+  P++P+  G+GH+DP +A
Sbjct: 581 AALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAA--PSSPYDHGAGHIDPLRA 640

Query: 601 FDPGLVYDITPQDYLNYLCSLNYTPSQIALLSR-GNFTCPSRRIVFLQPGDLNYPSFSVI 644
            DPGLVYDI PQ+Y  +LC+ + +PSQ+ + ++  N TC  +  +   PG+LNYP+ S +
Sbjct: 641 TDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC--KHTLAKNPGNLNYPAISAL 700

BLAST of Sgr018564 vs. ExPASy TrEMBL
Match: A0A6J1JPC3 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111487676 PE=3 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 9.3e-255
Identity = 487/734 (66.35%), Postives = 546/734 (74.39%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKMAA      +PEQ YTA+IDS+N++ SL +D+EEAS+AA+ILYVYKTAISGFAAK
Sbjct: 30  MDTTKMAA-----PSPEQWYTALIDSINKISSL-DDQEEASSAAQILYVYKTAISGFAAK 89

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           LSTKKLHSLSK PGFL+A P ELL                                    
Sbjct: 90  LSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGLQREHGLWNSSNLASDIVIGLLDT 149

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG C+ G KFS SNCN+KLIGA A++K YEA VGRLN T
Sbjct: 150 GIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCNRKLIGATAYIKGYEAIVGRLNAT 209

Query: 181 GSFRSPQDSDGPG-------------------HAQGI-------------DCCWN----- 240
           G+FRSP+DSDG G                    A G+               CW      
Sbjct: 210 GTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCAN 269

Query: 241 ----YAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPH 300
                AIDRAVADGVDV  LSLSL G  S FY DDIAIA FGAV+ GVFVSCSAGN GP 
Sbjct: 270 ADILAAIDRAVADGVDV--LSLSLGGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPS 329

Query: 301 PSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKI-ELPLVYNQSAGRQ 360
            STV N+APWIMTVAASY DR FP +VKLGNG+VFEGSSLYSGN I +LPLVYN +AG +
Sbjct: 330 SSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEGSSLYSGNSIGQLPLVYNNTAGGE 389

Query: 361 EANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADA 420
           EAN+CTA SLVPS+VK KIVVCERGTNSRT KGEQVKLAGGAGMILINT+L+GEEL AD 
Sbjct: 390 EANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADP 449

Query: 421 HILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEPN 480
           H+LPA +LGASAG+AI  YI+SS+  +PKA I FE TK+G+RAPRVAAFSSRGPS + P+
Sbjct: 450 HVLPAANLGASAGQAIIRYISSSK-HQPKALIAFEGTKFGNRAPRVAAFSSRGPSLIAPD 509

Query: 481 VIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAHK 540
           VIKPDVTAPGVNIL AWP I SPSEL+SDKR VLFNI+ GTSMSCPHVS L ALLKSAHK
Sbjct: 510 VIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSAHK 569

Query: 541 DWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYDI 600
           DWSPAAIKSALMTTAYT DN+MSPISDV S S  PANPF FGSGHVDP+KA DPGL+YDI
Sbjct: 570 DWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPANPFAFGSGHVDPEKASDPGLIYDI 629

Query: 601 TPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIM-KNARYVTI 647
           TPQDYLNY CSLNY  +QI L+SRGNFTCPS+R V  QPG+LNYPSFSV M K A+ V++
Sbjct: 630 TPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVG-QPGNLNYPSFSVFMKKKAKNVSV 689

BLAST of Sgr018564 vs. ExPASy TrEMBL
Match: A0A6J1FMR4 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111446877 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 4.6e-254
Identity = 487/735 (66.26%), Postives = 545/735 (74.15%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKMAA       PEQ YTA+IDS+NE+ SL ED+EEASNAA+ILYVYKTAISGFAAK
Sbjct: 30  MDTTKMAA-----PTPEQWYTALIDSINEISSL-EDQEEASNAAQILYVYKTAISGFAAK 89

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           LSTKKLHSLSK PGFL+A P ELL                                    
Sbjct: 90  LSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGLQREHGLWNSSNLASDIVIGLLDT 149

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG C+ G KFS SNCN+KLIGA A++K YEA VGRLN T
Sbjct: 150 GIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCNRKLIGAIAYIKGYEAIVGRLNAT 209

Query: 181 GSFRSPQDSDGPG-------------------HAQGI-------------DCCWN----- 240
           G+FRSP+DSDG G                    A G+               CW      
Sbjct: 210 GTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCAN 269

Query: 241 ----YAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPH 300
                AIDRAVADGVDV  LSLSL G  S FY DDIAIA FGAV+ GVFVSCSAGN GP 
Sbjct: 270 ADILAAIDRAVADGVDV--LSLSLGGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPS 329

Query: 301 PSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKI-ELPLVYNQSAGRQ 360
            STV N+APWIMTVAASY DR FP +VKLGNG+VFEGSSLYSGN I +LPLVYN +AG +
Sbjct: 330 SSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFEGSSLYSGNNIGQLPLVYNNTAGGE 389

Query: 361 EANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADA 420
           +AN+CTA SLVPSMVK KIVVCERGTNSRT KGEQVKLAGGAGMILINT+L+GEEL AD 
Sbjct: 390 DANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADP 449

Query: 421 HILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEPN 480
           H+LPA +LGASAG+AI  YI+SS+  +PKA I FE TK+G+RAPRVAAFSSRGPS + P+
Sbjct: 450 HVLPAANLGASAGQAIIRYISSSK-HQPKALIAFEGTKFGNRAPRVAAFSSRGPSLIAPD 509

Query: 481 VIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAHK 540
           VIKPDVTAPGVNIL AWP I SPSE++SDKR VLFN++ GTSMSCPHVS L ALLKSAHK
Sbjct: 510 VIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNVISGTSMSCPHVSGLAALLKSAHK 569

Query: 541 DWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYDI 600
           DWSPAAIKSALMTTAYT DN+MSPISDV SAS  PANPF FGSGHVDP+KA DPGL+YDI
Sbjct: 570 DWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPANPFAFGSGHVDPEKASDPGLIYDI 629

Query: 601 TPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIM-KNARYVTI 648
           TPQDYLNYLCSLNY  +QI L+SRGNFTCPS+R V  Q G LNYPSFSV M K A+ V++
Sbjct: 630 TPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVG-QAGKLNYPSFSVFMKKKAKNVSV 689

BLAST of Sgr018564 vs. ExPASy TrEMBL
Match: A0A6J1JN56 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111486706 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 3.0e-253
Identity = 476/734 (64.85%), Postives = 548/734 (74.66%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKMA       NPEQ YT++I S+N+L S+++D+ EASNAAEILY+YKTAISGF+AK
Sbjct: 31  MDATKMAT-----TNPEQWYTSIIHSINQLSSINDDQNEASNAAEILYIYKTAISGFSAK 90

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           LST+KLHSLSK+PGFL+A P++LL                                    
Sbjct: 91  LSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQFLGLQRGHGLWNASNLASDIIIGVIDT 150

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG C+ GPKFSRSNCNKKL+GARA++K YE  +GRLN T
Sbjct: 151 GIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSRSNCNKKLVGARAYIKGYETILGRLNTT 210

Query: 181 GSFRSPQDSDGPG-----------------HAQGIDC---------------CWN----- 240
           G+FRS +DSDG G                 + QG+                 CW      
Sbjct: 211 GTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMGAATGMRFTSRIAAYKVCWPEGCAS 270

Query: 241 ----YAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPH 300
                AIDRAV DGVDV  LSLSL G    FY D+IAIAAFGAV+ GVFVSCSAGN GP 
Sbjct: 271 TDILAAIDRAVVDGVDV--LSLSLGGGDGFFYQDEIAIAAFGAVRNGVFVSCSAGNSGPF 330

Query: 301 PSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKI-ELPLVYNQSAGRQ 360
            STV NVAPWIMTVAASY DR FP SVKLGNG++FEGSSL+SGN I ELPLVYN++AG +
Sbjct: 331 MSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIFEGSSLHSGNSIGELPLVYNKTAGGE 390

Query: 361 EANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADA 420
           EA++CTA SLVPSMVK KIVVCERGTNSR EKGEQVKLAGG GMILINT+L+GEEL  D+
Sbjct: 391 EASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVKLAGGVGMILINTQLEGEELFGDS 450

Query: 421 HILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEPN 480
           H+LPA +LGASAGKAI +YI SS+   PKASI+FE T+YGSRAPR+AAFSSRGPS  EP 
Sbjct: 451 HVLPAINLGASAGKAIINYIASSK-HPPKASILFEGTRYGSRAPRMAAFSSRGPSFFEPY 510

Query: 481 VIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAHK 540
           VIKPD+TAPGVNIL AWPP++SPSELKSDKR VLFNI+ GTSMSCPHVS + ALLKSAHK
Sbjct: 511 VIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNIISGTSMSCPHVSGIAALLKSAHK 570

Query: 541 DWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYDI 600
           +WSPAAIKSALMTTAY  DNK S ISDV   S  PA+P+ FGSGHVDP+KA DPGLVYDI
Sbjct: 571 NWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGPADPYAFGSGHVDPEKAVDPGLVYDI 630

Query: 601 TPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIMKNARYVTIT 648
            PQDYLNYLCSLNYT  Q+ L+SRGNF+CPS+R V LQPGDLNYPSFSV MKN +   + 
Sbjct: 631 VPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTV-LQPGDLNYPSFSVSMKN-KAKNVR 690

BLAST of Sgr018564 vs. ExPASy TrEMBL
Match: A0A6J1JRF2 (subtilisin-like protease SBT1.1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487664 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 9.6e-252
Identity = 482/734 (65.67%), Postives = 546/734 (74.39%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKM A      +PEQ Y+AMI SVN++ SLH+DEEEASNAAEILYVYKTAISGFAAK
Sbjct: 43  MDTTKMVA----TTSPEQWYSAMIHSVNKISSLHQDEEEASNAAEILYVYKTAISGFAAK 102

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           L+TKKLHSLSK+PGFL+A P+ELL                                    
Sbjct: 103 LTTKKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLHREHGLWNSSNSASDIIIGLIDS 162

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG CE GPKFS SNCNKKL+GARA++  YEA VGRLN T
Sbjct: 163 GIWPEHISFQDKGLPPVPKKWKGACEAGPKFSPSNCNKKLVGARAYIDGYEAIVGRLNVT 222

Query: 181 GSFRSPQDSDGPG-----------------HAQG---------------IDCCWNY---- 240
           G+FRSP+DS+G G                 ++QG                  CW      
Sbjct: 223 GTFRSPRDSNGHGTHTASTAAGNIVNKASLYSQGKGVATGISFTSRIAAYKACWPMGCAN 282

Query: 241 -----AIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGP- 300
                A+DRAVADGVD+  LSLSL G  + FY D+IAIAAFGAVQ GVFVSCSAGN+G  
Sbjct: 283 ADILAAMDRAVADGVDI--LSLSLGGGVTAFYKDNIAIAAFGAVQNGVFVSCSAGNFGSL 342

Query: 301 HPSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSG-NKIELPLVYNQSAGR 360
             S V N APWIMTVAASY DR FPT+VKLGNG+VFEGSSLYSG N  ELPLVYN + G 
Sbjct: 343 FRSAVSNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYSGQNTAELPLVYNNTVGG 402

Query: 361 QEANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVAD 420
           QEANLCT  SLVP+MVK KIVVC RG N R  KGEQVKLAGGAGMILINT+LDGEEL A+
Sbjct: 403 QEANLCTPGSLVPAMVKGKIVVCGRGKNPRVAKGEQVKLAGGAGMILINTQLDGEELFAE 462

Query: 421 AHILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEP 480
            H+LPATSLGASA KA+  YI SS+ ++P ASI F+ TKYGSRAP+VAAFSSRGPS + P
Sbjct: 463 PHVLPATSLGASATKAMLDYIASSK-NQPTASIGFQGTKYGSRAPKVAAFSSRGPSLIGP 522

Query: 481 NVIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAH 540
           +VIKPDVTAPGVNIL AWPPI+SPSEL+ DKR VLFNI+ GTSMSCPHVS L AL+KSAH
Sbjct: 523 DVIKPDVTAPGVNILAAWPPIVSPSELEFDKRRVLFNIISGTSMSCPHVSGLAALIKSAH 582

Query: 541 KDWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYD 600
            +WSPAAIKSALMTTAYTTDNKMSPISDVS  +  PA+PF  GSGHVDP+KA DPGLVYD
Sbjct: 583 NNWSPAAIKSALMTTAYTTDNKMSPISDVSHPNGEPADPFAIGSGHVDPEKASDPGLVYD 642

Query: 601 ITPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIMKNARYVTI 647
           I P DYLNYLCSLNY+ +QI LLS+GNF+CPS+R V ++PGDLNYPSFSVIMK A+ V++
Sbjct: 643 IAPGDYLNYLCSLNYSSTQIGLLSKGNFSCPSKREV-VEPGDLNYPSFSVIMK-AKNVSV 702

BLAST of Sgr018564 vs. ExPASy TrEMBL
Match: A0A6J1JUQ4 (subtilisin-like protease SBT1.1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487664 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 9.6e-252
Identity = 482/734 (65.67%), Postives = 546/734 (74.39%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MD TKM A      +PEQ Y+AMI SVN++ SLH+DEEEASNAAEILYVYKTAISGFAAK
Sbjct: 31  MDTTKMVA----TTSPEQWYSAMIHSVNKISSLHQDEEEASNAAEILYVYKTAISGFAAK 90

Query: 61  LSTKKLHSLSKMPGFLSANPEELL------------------------------------ 120
           L+TKKLHSLSK+PGFL+A P+ELL                                    
Sbjct: 91  LTTKKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLHREHGLWNSSNSASDIIIGLIDS 150

Query: 121 ---------QDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNET 180
                    QD+GLPP P +WKG CE GPKFS SNCNKKL+GARA++  YEA VGRLN T
Sbjct: 151 GIWPEHISFQDKGLPPVPKKWKGACEAGPKFSPSNCNKKLVGARAYIDGYEAIVGRLNVT 210

Query: 181 GSFRSPQDSDGPG-----------------HAQG---------------IDCCWNY---- 240
           G+FRSP+DS+G G                 ++QG                  CW      
Sbjct: 211 GTFRSPRDSNGHGTHTASTAAGNIVNKASLYSQGKGVATGISFTSRIAAYKACWPMGCAN 270

Query: 241 -----AIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGP- 300
                A+DRAVADGVD+  LSLSL G  + FY D+IAIAAFGAVQ GVFVSCSAGN+G  
Sbjct: 271 ADILAAMDRAVADGVDI--LSLSLGGGVTAFYKDNIAIAAFGAVQNGVFVSCSAGNFGSL 330

Query: 301 HPSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSG-NKIELPLVYNQSAGR 360
             S V N APWIMTVAASY DR FPT+VKLGNG+VFEGSSLYSG N  ELPLVYN + G 
Sbjct: 331 FRSAVSNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYSGQNTAELPLVYNNTVGG 390

Query: 361 QEANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVAD 420
           QEANLCT  SLVP+MVK KIVVC RG N R  KGEQVKLAGGAGMILINT+LDGEEL A+
Sbjct: 391 QEANLCTPGSLVPAMVKGKIVVCGRGKNPRVAKGEQVKLAGGAGMILINTQLDGEELFAE 450

Query: 421 AHILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEP 480
            H+LPATSLGASA KA+  YI SS+ ++P ASI F+ TKYGSRAP+VAAFSSRGPS + P
Sbjct: 451 PHVLPATSLGASATKAMLDYIASSK-NQPTASIGFQGTKYGSRAPKVAAFSSRGPSLIGP 510

Query: 481 NVIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAH 540
           +VIKPDVTAPGVNIL AWPPI+SPSEL+ DKR VLFNI+ GTSMSCPHVS L AL+KSAH
Sbjct: 511 DVIKPDVTAPGVNILAAWPPIVSPSELEFDKRRVLFNIISGTSMSCPHVSGLAALIKSAH 570

Query: 541 KDWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYD 600
            +WSPAAIKSALMTTAYTTDNKMSPISDVS  +  PA+PF  GSGHVDP+KA DPGLVYD
Sbjct: 571 NNWSPAAIKSALMTTAYTTDNKMSPISDVSHPNGEPADPFAIGSGHVDPEKASDPGLVYD 630

Query: 601 ITPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIMKNARYVTI 647
           I P DYLNYLCSLNY+ +QI LLS+GNF+CPS+R V ++PGDLNYPSFSVIMK A+ V++
Sbjct: 631 IAPGDYLNYLCSLNYSSTQIGLLSKGNFSCPSKREV-VEPGDLNYPSFSVIMK-AKNVSV 690

BLAST of Sgr018564 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 585.5 bits (1508), Expect = 5.5e-167
Identity = 334/714 (46.78%), Postives = 431/714 (60.36%), Query Frame = 0

Query: 23  MIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAKLSTKKLHSLSKMPGFLSANPEE 82
           ++ S+   L      ++  +  EI Y+Y+ A+SGF+A L+  +L ++    GF+SA P+E
Sbjct: 56  IVTSLFNSLQTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDE 115

Query: 83  LLQ---------------------------------DEGLPPE------------PARWK 142
           LL                                  D G+ PE            P+RW+
Sbjct: 116 LLSLHTTYSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWR 175

Query: 143 GVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNETGSFRSPQDSDGP---------- 202
           G C+EG  FS S CNKK+IGA AF K YE+ VG++NET  FRS +D+ G           
Sbjct: 176 GSCDEGTNFSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAG 235

Query: 203 ---------GHAQGI-------------DCCWNY---------AIDRAVADGVDVLSLSL 262
                    G A+G+               CW           AIDRA+ DGVDV  +SL
Sbjct: 236 DIVPKANYFGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDV--ISL 295

Query: 263 SLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPHPSTVDNVAPWIMTVAASYIDRI 322
           SL G + PFY D IAIA FGA+QK +FVSCSAGN GP  STV N APW+MTVAASY DR 
Sbjct: 296 SLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRT 355

Query: 323 FPTSVKLGNGEVFEGSSLYSGNKIE-LPLVYNQSAGRQE-ANLCTASSLVPSMVKRKIVV 382
           FP  V++GN +   GSSLY G  ++ LPL +N++AG +  A  C   SL   +V+ KIV+
Sbjct: 356 FPAIVRIGNRKSLVGSSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVI 415

Query: 383 CERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADAHILPATSLGASAGKAIKSYIT 442
           C RG + RT KGE+VK +GGA M+L++TE +GEEL+AD H+LPA SLG S GK + +Y+ 
Sbjct: 416 CLRGASGRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLA 475

Query: 443 SSRDSKPKASIVFEQTKYGSRAPRVAAFSSRGPSSVEPNVIKPDVTAPGVNILVAWPPII 502
            + ++   AS+ F  T YG+ AP VAAFSSRGPS   P + KPD+ APG+NIL  W P  
Sbjct: 476 GAANA--TASVRFRGTAYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFS 535

Query: 503 SPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAHKDWSPAAIKSALMTTAYTTDNK 562
           SPS L+SD R V FNI+ GTSM+CPH+S + AL+KS H DWSPA IKSA+MTTA  TDN+
Sbjct: 536 SPSLLRSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNR 595

Query: 563 MSPISDVSSA-SFVPANPFTFGSGHVDPKKAFDPGLVYDITPQDYLNYLCSLNYTPSQIA 622
             PI D  +A +   A  F FG+G+VDP +A DPGLVYD +  DYLNYLCSLNYT  +I 
Sbjct: 596 NRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERIL 655

Query: 623 LLSRGNFTCPSRRIVFLQPGDLNYPSFSV-IMKNARYVTITFKRTVTNVGSPSSVYTVQI 647
           L S  N+TC S  +V L PGDLNYPSF+V ++  A   T+ +KRTVTNVGSP+  Y V +
Sbjct: 656 LFSGTNYTCASNAVV-LSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHV 715

BLAST of Sgr018564 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 520.0 bits (1338), Expect = 2.8e-147
Identity = 301/617 (48.78%), Postives = 378/617 (61.26%), Query Frame = 0

Query: 86  DEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLNETGSFRSPQDSD 145
           D GL P P +WKG C     F  S CN+KL+GAR F   YEA  G++NET  FRSP+DSD
Sbjct: 149 DRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSD 208

Query: 146 GP-------------------GHAQGI-------------DCCWN---------YAIDRA 205
           G                    G+A G+               CWN          A D A
Sbjct: 209 GHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTA 268

Query: 206 VADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYGPHPSTVDNVAPW 265
           VADGVDV  +SLS+ G   P+Y D IAI AFGA+ +G+FVS SAGN GP   TV NVAPW
Sbjct: 269 VADGVDV--ISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPW 328

Query: 266 IMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKIE----LPLVYNQS---AGRQEANL 325
           + TV A  IDR FP +VKLGNG++  G S+Y G  ++     PLVY  S        ++L
Sbjct: 329 MTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSL 388

Query: 326 CTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELDGEELVADAHILP 385
           C   SL P++VK KIV+C+RG NSR  KGE V+  GG GMI+ N   DGE LVAD H+LP
Sbjct: 389 CLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLP 448

Query: 386 ATSLGASAGKAIKSYITSSRDSK----PKASIVFEQTKYGSR-APRVAAFSSRGPSSVEP 445
           ATS+GAS G  I+ YI+ S  S+    P A+IVF+ T+ G R AP VA+FS+RGP+   P
Sbjct: 449 ATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETP 508

Query: 446 NVIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSLVALLKSAH 505
            ++KPDV APG+NIL AWP  I PS + SD R   FNI+ GTSM+CPHVS L ALLK+AH
Sbjct: 509 EILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAH 568

Query: 506 KDWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKAFDPGLVYD 565
            DWSPAAI+SAL+TTAYT DN   P+ D S+ +   ++   +GSGHV P KA DPGLVYD
Sbjct: 569 PDWSPAAIRSALITTAYTVDNSGEPMMDESTGN--TSSVMDYGSGHVHPTKAMDPGLVYD 628

Query: 566 ITPQDYLNYLCSLNYTPSQIALLSRGNFTCPSRRIVFLQPGDLNYPSFSVIMK--NARYV 625
           IT  DY+N+LC+ NYT + I  ++R    C   R      G+LNYPSFSV+ +      +
Sbjct: 629 ITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAG-HVGNLNYPSFSVVFQQYGESKM 688

Query: 626 TITFKRTVTNVGSPSSVYTVQINNPKGVAITVKPKKLSFVRLGEKLSYQVSFVALEKKEA 646
           +  F RTVTNVG   SVY ++I  P+G  +TV+P+KLSF R+G+KLS+ V     E K +
Sbjct: 689 STHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLS 748

BLAST of Sgr018564 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 511.9 bits (1317), Expect = 7.8e-145
Identity = 304/700 (43.43%), Postives = 403/700 (57.57%), Query Frame = 0

Query: 41  SNAAEILYVYKTAISGFAAKLSTKKLHSLSKMPGFLSANPEELLQ--------------- 100
           S++AE+LY Y+ AI GF+ +L+ ++  SL   PG +S  PE   +               
Sbjct: 60  SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 119

Query: 101 -------------------------------DEGLPPEPARWKGVCEEGPKFSRSNCNKK 160
                                          DEG  P P+ WKG CE G  F+ S CN+K
Sbjct: 120 TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRK 179

Query: 161 LIGARAFLKAYEAGVGRLNETGSFRSPQDSDGP-------------------GHAQG--- 220
           LIGAR F + YE+ +G ++E+   RSP+D DG                    G+A G   
Sbjct: 180 LIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 239

Query: 221 ----------IDCCW---------NYAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIA 280
                        CW           AID+A+AD V+V  LS+SL G  S +Y D +AI 
Sbjct: 240 GMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNV--LSMSLGGGMSDYYRDGVAIG 299

Query: 281 AFGAVQKGVFVSCSAGNYGPHPSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSS 340
           AF A+++G+ VSCSAGN GP  S++ NVAPWI TV A  +DR FP    LGNG+ F G S
Sbjct: 300 AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 359

Query: 341 LYSGNKIE---LPLVY-NQSAGRQEANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQV 400
           L+ G  +    LP +Y   ++     NLC   +L+P  VK KIV+C+RG N+R +KG+ V
Sbjct: 360 LFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVV 419

Query: 401 KLAGGAGMILINTELDGEELVADAHILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQ 460
           K AGG GMIL NT  +GEELVADAH+LPAT++G  AG  I+ Y+T+  D  P ASI    
Sbjct: 420 KAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTT--DPNPTASISILG 479

Query: 461 TKYGSR-APRVAAFSSRGPSSVEPNVIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLF 520
           T  G + +P VAAFSSRGP+S+ PN++KPD+ APGVNIL AW     P+ L SD R V F
Sbjct: 480 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 539

Query: 521 NIVLGTSMSCPHVSSLVALLKSAHKDWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVP 580
           NI+ GTSMSCPHVS L ALLKS H +WSPAAI+SALMTTAY T     P+ D+++    P
Sbjct: 540 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK--P 599

Query: 581 ANPFTFGSGHVDPKKAFDPGLVYDITPQDYLNYLCSLNYTPSQIALLSRGNFTC-PSRRI 640
           + PF  G+GHV P  A +PGL+YD+T +DYL +LC+LNYT  QI  +SR N+TC PS+  
Sbjct: 600 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS- 659

Query: 641 VFLQPGDLNYPSFSVIMKNARYVTITFKRTVTNVGSPSSVYTVQI-NNPKGVAITVKPKK 647
                 DLNYPSF+V +         + RTVT+VG  +  Y+V++ +   GV I+V+P  
Sbjct: 660 --YSVADLNYPSFAVNVDGVG--AYKYTRTVTSVGG-AGTYSVKVTSETTGVKISVEPAV 719

BLAST of Sgr018564 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 506.5 bits (1303), Expect = 3.3e-143
Identity = 305/696 (43.82%), Postives = 396/696 (56.90%), Query Frame = 0

Query: 39  EASNAAEILYVYKTAISGFAAKLSTKKLHSL--------------------SKMPGFLSA 98
           + ++ + +LY Y T+  GF+A L + +  SL                    ++ P FL  
Sbjct: 53  QLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL 112

Query: 99  NPEELLQDEG--------------------------LPPEPARWKGVCEEGPKFSRSNCN 158
           N E  + D G                          +P  P++WKG CE G  F    CN
Sbjct: 113 NSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCN 172

Query: 159 KKLIGARAFLKAYE-AGVGRLNETGSFRSPQDSDG-----------------------PG 218
           KKLIGAR+F K ++ A  G  +      SP+D DG                        G
Sbjct: 173 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 232

Query: 219 HAQGI---------DCCWN---------YAIDRAVADGVDVLSLSLSLNGDTSPFYSDDI 278
            A+G+           CW+          A+DRA+ DGVDV  LSLSL G ++P+Y D I
Sbjct: 233 TARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDV--LSLSLGGGSAPYYRDTI 292

Query: 279 AIAAFGAVQKGVFVSCSAGNYGPHPSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFE 338
           AI AF A+++GVFVSCSAGN GP  ++V NVAPW+MTV A  +DR FP    LGNG+   
Sbjct: 293 AIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLT 352

Query: 339 GSSLYSG---NKIELPLVYNQSAGRQEANLCTASSLVPSMVKRKIVVCERGTNSRTEKGE 398
           G SLYSG       L LVYN+      +NLC   SL  S+V+ KIVVC+RG N+R EKG 
Sbjct: 353 GVSLYSGVGMGTKPLELVYNK-GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGA 412

Query: 399 QVKLAGGAGMILINTELDGEELVADAHILPATSLGASAGKAIKSYITSSRDSKPKASIVF 458
            V+ AGG GMI+ NT   GEELVAD+H+LPA ++G   G  ++ Y+ S  DSKP A +VF
Sbjct: 413 VVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKS--DSKPTALLVF 472

Query: 459 EQTKYGSR-APRVAAFSSRGPSSVEPNVIKPDVTAPGVNILVAWPPIISPSELKSDKRSV 518
           + T    + +P VAAFSSRGP++V P ++KPDV  PGVNIL  W   I P+ L  D R  
Sbjct: 473 KGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRT 532

Query: 519 LFNIVLGTSMSCPHVSSLVALLKSAHKDWSPAAIKSALMTTAYTTDNKMSPISDVSSASF 578
            FNI+ GTSMSCPH+S L  LLK+AH +WSP+AIKSALMTTAY  DN  +P+ D +  S 
Sbjct: 533 QFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSL 592

Query: 579 VPANPFTFGSGHVDPKKAFDPGLVYDITPQDYLNYLCSLNYTPSQI-ALLSRGNFTCPSR 638
             +NP+  GSGHVDP+KA  PGLVYDI+ ++Y+ +LCSL+YT   I A++ R +  C  +
Sbjct: 593 --SNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK 652

Query: 639 RIVFLQPGDLNYPSFSVIMKNARYVTITFKRTVTNVGSPSSVYTVQINNPKGVAITVKPK 642
              F  PG LNYPSFSV+    R V  T  R VTNVG+ SSVY V +N    V I+VKP 
Sbjct: 653 ---FSDPGQLNYPSFSVLFGGKRVVRYT--REVTNVGAASSVYKVTVNGAPSVGISVKPS 712

BLAST of Sgr018564 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 504.2 bits (1297), Expect = 1.6e-142
Identity = 303/741 (40.89%), Postives = 417/741 (56.28%), Query Frame = 0

Query: 1   MDKTKMAAIGHGEANPEQGYTAMIDSVNELLSLHEDEEEASNAAEILYVYKTAISGFAAK 60
           MDK+ M        N  Q Y++ I+SV +    H+ +EE  N   ILY Y+TA  G AA+
Sbjct: 41  MDKSAMPL---PYTNHLQWYSSKINSVTQ----HKSQEEEGNNNRILYTYQTAFHGLAAQ 100

Query: 61  LSTKKLHSLSKMPGFLSANPE--------------------------------------- 120
           L+ ++   L +  G ++  PE                                       
Sbjct: 101 LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 160

Query: 121 --------ELLQDEGLPPEPARWKGVCEEGPKFSRSNCNKKLIGARAFLKAYEAGVGRLN 180
                   E   D G+ P PA W+G CE G +F + NCN+K++GAR F + YEA  G+++
Sbjct: 161 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 220

Query: 181 ETGSFRSPQDSDGP-----------------------GHAQGI---------DCCW---- 240
           E   ++SP+D DG                        G A+G+           CW    
Sbjct: 221 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 280

Query: 241 -----NYAIDRAVADGVDVLSLSLSLNGDTSPFYSDDIAIAAFGAVQKGVFVSCSAGNYG 300
                  A+D+AVADGV V  LS+SL G  S +  D ++IA FGA++ GVFVSCSAGN G
Sbjct: 281 FSSDILSAVDQAVADGVQV--LSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGG 340

Query: 301 PHPSTVDNVAPWIMTVAASYIDRIFPTSVKLGNGEVFEGSSLYSGNKI-----ELPLVY- 360
           P P ++ NV+PWI TV AS +DR FP +VK+G    F+G SLY G  +     + PLVY 
Sbjct: 341 PDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYL 400

Query: 361 -NQSAGRQEANLCTASSLVPSMVKRKIVVCERGTNSRTEKGEQVKLAGGAGMILINTELD 420
              ++     + C   +L    V  KIV+C+RG   R +KG+ VK AGG GM+L NT  +
Sbjct: 401 GRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATN 460

Query: 421 GEELVADAHILPATSLGASAGKAIKSYITSSRDSKPKASIVFEQTKYGSR-APRVAAFSS 480
           GEELVAD+H+LPA ++G   GK IK Y  +S+  K  AS+    T+ G + +P VAAFSS
Sbjct: 461 GEELVADSHMLPAVAVGEKEGKLIKQYAMTSK--KATASLEILGTRIGIKPSPVVAAFSS 520

Query: 481 RGPSSVEPNVIKPDVTAPGVNILVAWPPIISPSELKSDKRSVLFNIVLGTSMSCPHVSSL 540
           RGP+ +   ++KPD+ APGVNIL AW   ++PS L SD R V FNI+ GTSMSCPHVS +
Sbjct: 521 RGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGV 580

Query: 541 VALLKSAHKDWSPAAIKSALMTTAYTTDNKMSPISDVSSASFVPANPFTFGSGHVDPKKA 600
            AL+KS H DWSPAAIKSALMTTAY  DN   P++D S A+  P++P+  G+GH+DP +A
Sbjct: 581 AALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAA--PSSPYDHGAGHIDPLRA 640

Query: 601 FDPGLVYDITPQDYLNYLCSLNYTPSQIALLSR-GNFTCPSRRIVFLQPGDLNYPSFSVI 644
            DPGLVYDI PQ+Y  +LC+ + +PSQ+ + ++  N TC  +  +   PG+LNYP+ S +
Sbjct: 641 TDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC--KHTLAKNPGNLNYPAISAL 700